CN104419645A - Probiotic liquid specially used for banana tree plantation - Google Patents

Probiotic liquid specially used for banana tree plantation Download PDF

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Publication number
CN104419645A
CN104419645A CN201310378254.2A CN201310378254A CN104419645A CN 104419645 A CN104419645 A CN 104419645A CN 201310378254 A CN201310378254 A CN 201310378254A CN 104419645 A CN104419645 A CN 104419645A
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China
Prior art keywords
banana tree
banana
probiotic liquid
milk
peptone
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Pending
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CN201310378254.2A
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Chinese (zh)
Inventor
王海玲
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Qingdao Medical Prevention Disinfection Professional Technology Center
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Qingdao Medical Prevention Disinfection Professional Technology Center
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Priority to CN201310378254.2A priority Critical patent/CN104419645A/en
Publication of CN104419645A publication Critical patent/CN104419645A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Agronomy & Crop Science (AREA)
  • Biomedical Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a probiotic liquid specially used for banana tree plantation, and belongs to the technical field of crop plantation. The probiotic liquid is prepared by using agar powder, Enteromorpha prolifera polysaccharide extract, actinomyces, bifidobacteria, yeast, Bacillus subtilis, lactobacillus, peptone, brown sugar, sodium chloride and soybean milk through a fermentation culture technology. The probiotic liquid is suitable for banana tree plantation and cultivation environment reconstruction. Compared with existing products, the probiotic liquid has the advantages of soil environment improvement, air purification, decomposition of nutritional substances in soil, growth absorption promotion, effective reduction of the propagation of harmful strains, and disease prevention.

Description

Banana tree plants special probiotic bacterium liquid
technical fieldthe present invention relates to a kind of Banana tree and plant special probiotic bacterium liquid, form primarily of agar powder, sea grass polysaccharide extract, actinomycetes, bifidus bacillus, yeast, Bacillus subtilus, milk-acid bacteria, peptone, brown sugar, sodium-chlor, soya-bean milk fermentating culturing process, belong to proportion of crop planting technical field.Be applicable to Banana tree plantation and nurturing an environment transformation.Along with the continuous lifting of China's banana industry, Optimization Planting environmental management mode becomes one of the essential condition in contention banana market.The high-density planting of Banana tree, causes environmental degradation, and in order to improve planting environment, peasant, by splashing, sprays a large amount of disinfection agricultural chemicals to reduce the incidence of banana disease.Although this measure can play certain emergency processing effect, if but in life-time service medicament, easy generation resistance, and grow under the environment that air pesticide concentration is higher for a long time, the product produced also easily adheres to the residuals of agricultural chemicals, once eat human body, the health of human body directly can be jeopardized.Therefore, we need one improving Banana tree planting environment, have the nontoxic preparation residual without agriculture simultaneously.The present invention adopts the compounded Banana tree of multiple beneficial probiotic bacterium to plant special probiotic bacterium liquid, by changing the bacterium colony population ratio in planting soil, cultivate the probiotic bacterium dominant colony that can not affect and endanger banana and cultivate, effectively can improve and utilize the nutritive ingredient in soil, promote the growth of farm crop, also have the effect of purification banana woods air ambient.
background technologya kind of novel Banana tree is the object of the present invention is to provide to plant special probiotic bacterium liquid.Due to the fast development of banana industry, the mode of high-density planting Banana tree is comparatively common, can save cost, reaches the profit that results are larger.But, once mismanagement, just can run counter to desire.High-density cultivates Banana tree, cause the temperature in banana woods all can raise with relative moisture absorption, daylight also cannot be able to be irradiated on soil because of the intensive reason of banana leaf, under this dark environment, perfect condition will be created for rapid microbial growth, the quick growth and breeding of harmful microbe is the arch-criminal of the disease causing a series of bacillary and fungoid, as banana bunchy top disease, Banana Mosaic Disease, banana sooty mould, human skeleton disease, black spot, sigatoka, banana crown rot, the bacillary heartrot of banana etc.The microbial environment that the breeding of the pathogenic micro-organism of these diseases all grows with Banana tree is closely bound up.Chemical agent agricultural chemicals is adopted to process to Banana tree disease major part at present, easily damage the living environment of vegetation, cause more side effect, in order to better solve the side-effect problem that chemical agent agricultural chemicals brings, the invention provides a kind of Banana tree and plant special probiotic bacterium liquid, by cultivating dominant bacteria bacterium colony, fighting for other microbial growth nutrition and becoming into, the breeding effectively suppressing pathogenic bacterium bacterium colony can be played, the generation of disease preventing and treating.In addition, the present invention also has multiple advantage, by decomposing the septic matter in soil, nutritive ingredient needed for Banana tree growth can be provided, eliminate the corrupt smell in air, the thorough planting environment improving Banana tree, or the good recipe of the plantation of a kind of Multifunction Banana tree and nurturing an environment transformation.
summary of the inventionthe object of the present invention is to provide a kind of special probiotic bacterium liquid with multi-functional banana tree planting, the proportioning mode had most by selection, is carried out rational compatibility, assembles.
The formula that the present invention selects following (, g/l): 1, actinomycetes, bifidus bacillus 1,1, yeast, 1, Bacillus subtilus, milk-acid bacteria 1 are inoculated in agar powder 5-10; Sea grass polysaccharide extract 50-70; Peptone 20-30; Brown sugar 150-180; Sodium-chlor 15-20; Soya-bean milk 50-60; Sterilized water is to 1000ml.
Feature of the present invention is that the different special bacterial classification of screening 5 kinds carries out mixed culture, by complementary action principle, impels development breeding faster more orderly between bacterial classification.Not only can not work the mischief to Banana tree, strong advantage can be formed under certain proportion, take by biology the breeding that oxygen mode stops pathogenic bacteria by force; Or kill pathogenic micro-organism by generation meta-bolites and physiologically active substance, as produced the pH value in organic acid reduction soil, kill acid nonfast thalline; The bacteriocin that the acidophilin also having soft acid bacterium to produce, lactobacillin and Bacillus subtilus produce all has obvious effect.
Sea grass polysaccharide extract: there is great amount of hydroxy group and carboxyl isopolarity group in its structure, can be cross-linked with each other in reticulated structure with water molecules stroke hydrogen bond, show good moisture absorption and performance of keeping humidity, and ambient moisture is less on the impact of its rate of moisture absorption, is a kind of natural wetting Agent for Printing Inks; Not only can provide plant water keeping function, also can be used as the growing carrier of bacterium liquid, nurse tree body.
Agar powder, peptone, brown sugar, sodium-chlor, soya-bean milk as providing the growth of probiotic bacterium must nutritive substance and growing carrier,
The preparation method of product of the present invention is:
1) in proportion agar powder, peptone, sea grass polysaccharide extract, brown sugar, sodium-chlor, soya-bean milk, water 1000ml are dissolved mixture, packing;
2) high-temperature sterilization, cools for subsequent use;
3) be inoculated in above-mentioned substratum respectively by actinomycetes, bifidus bacillus, yeast, Bacillus subtilus, milk-acid bacteria, 37 DEG C of 24-48 hours fermentation are cultivated, the packing of nutrient solution keg.
The present invention compares with existing product and has the following advantages: (1) improves edatope, purifies air; (2) nutritive substance in decomposable asymmetric choice net soil, growth promotion absorbs; (3) breeding of harmful bacterial classification is effectively reduced, prophylactic generation.
embodimentbelow in conjunction with embodiment, the present invention is described in further detail:
To produce 100k, be configured according to following data and described step thereof:
1, fill a prescription (, g/l): 1, actinomycetes, bifidus bacillus 1,1, yeast, 1, Bacillus subtilus, milk-acid bacteria 1 are inoculated in agar powder 5-7; Sea grass polysaccharide extract 60-70; Peptone 24-30; Brown sugar 150-165; Sodium-chlor 18-20; Soya-bean milk 55-60; Sterilized water is to 1000ml.
2. preparation method:
1) in proportion agar powder, peptone, sea grass polysaccharide extract, brown sugar, sodium-chlor, soya-bean milk, water 1000ml are dissolved mixture, packing;
2) high-temperature sterilization, cools for subsequent use;
3) be inoculated in above-mentioned substratum respectively by actinomycetes, bifidus bacillus, yeast, Bacillus subtilus, milk-acid bacteria, 37 DEG C of 24-48 hours fermentation are cultivated, the packing of nutrient solution keg.

Claims (2)

1. the present invention relates to a kind of Banana tree and plant special probiotic bacterium liquid, it is characterized in that having following formula (, g/l): one, actinomycetes, bifidus bacillus 1,1, yeast, 1, Bacillus subtilus, milk-acid bacteria 1 are inoculated in agar powder 5-10, sea grass polysaccharide extract 50-70, peptone 20-30, brown sugar 150-180, sodium-chlor 15-20, soya-bean milk 50-60, sterilized water to 1000ml.
2. Banana tree according to claim 1 plants special probiotic bacterium liquid, it is characterized in that having following technique:
1) in proportion agar powder, peptone, sea grass polysaccharide extract, brown sugar, sodium-chlor, soya-bean milk, water 1000ml are dissolved mixture, packing;
2) high-temperature sterilization, cools for subsequent use;
3) be inoculated in above-mentioned substratum respectively by actinomycetes, bifidus bacillus, yeast, Bacillus subtilus, milk-acid bacteria, 37 DEG C of 24-48 hours fermentation are cultivated, the packing of nutrient solution keg.
CN201310378254.2A 2013-08-28 2013-08-28 Probiotic liquid specially used for banana tree plantation Pending CN104419645A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310378254.2A CN104419645A (en) 2013-08-28 2013-08-28 Probiotic liquid specially used for banana tree plantation

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310378254.2A CN104419645A (en) 2013-08-28 2013-08-28 Probiotic liquid specially used for banana tree plantation

Publications (1)

Publication Number Publication Date
CN104419645A true CN104419645A (en) 2015-03-18

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CN201310378254.2A Pending CN104419645A (en) 2013-08-28 2013-08-28 Probiotic liquid specially used for banana tree plantation

Country Status (1)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105075586A (en) * 2015-07-25 2015-11-25 安徽博发文化生态园有限公司 Planting method of muskmelon
CN114868572A (en) * 2022-04-29 2022-08-09 景宁金锄农业发展有限公司 Method for preventing and treating canna edulis ker diseases

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105075586A (en) * 2015-07-25 2015-11-25 安徽博发文化生态园有限公司 Planting method of muskmelon
CN114868572A (en) * 2022-04-29 2022-08-09 景宁金锄农业发展有限公司 Method for preventing and treating canna edulis ker diseases

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Application publication date: 20150318

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