CN104404035B - 一种棉花抗黄萎病est‑ssr分子标记及其制备方法 - Google Patents
一种棉花抗黄萎病est‑ssr分子标记及其制备方法 Download PDFInfo
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- CN104404035B CN104404035B CN201410230026.5A CN201410230026A CN104404035B CN 104404035 B CN104404035 B CN 104404035B CN 201410230026 A CN201410230026 A CN 201410230026A CN 104404035 B CN104404035 B CN 104404035B
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- cotton
- verticillium
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- ssr molecular
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 78
- 241000082085 Verticillium <Phyllachorales> Species 0.000 title claims abstract description 71
- 238000002360 preparation method Methods 0.000 title description 6
- 238000002372 labelling Methods 0.000 claims abstract description 44
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 9
- 239000003147 molecular marker Substances 0.000 claims description 5
- 239000000463 material Substances 0.000 abstract description 10
- 238000001514 detection method Methods 0.000 abstract description 2
- 241000219146 Gossypium Species 0.000 description 72
- 108090000623 proteins and genes Proteins 0.000 description 14
- 240000000047 Gossypium barbadense Species 0.000 description 12
- 235000009429 Gossypium barbadense Nutrition 0.000 description 11
- 108020004414 DNA Proteins 0.000 description 9
- 238000009395 breeding Methods 0.000 description 8
- 230000001488 breeding effect Effects 0.000 description 8
- 235000018322 upland cotton Nutrition 0.000 description 8
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 230000002068 genetic effect Effects 0.000 description 7
- 241000219194 Arabidopsis Species 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 239000003550 marker Substances 0.000 description 5
- 235000009438 Gossypium Nutrition 0.000 description 4
- 240000001814 Gossypium arboreum Species 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 108700028369 Alleles Proteins 0.000 description 3
- 235000004342 Gossypium thurberi Nutrition 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 244000070990 Gomphocarpus physocarpus Species 0.000 description 2
- 240000002024 Gossypium herbaceum Species 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000218052 Gossypium anomalum Species 0.000 description 1
- 241001149081 Gossypium raimondii Species 0.000 description 1
- 241001479477 Gossypium sturtianum Species 0.000 description 1
- 101000832077 Xenopus laevis Dapper 1-A Proteins 0.000 description 1
- 230000036579 abiotic stress Effects 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000003234 polygenic effect Effects 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 238000009941 weaving Methods 0.000 description 1
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN201410230026.5A CN104404035B (zh) | 2014-05-28 | 2014-05-28 | 一种棉花抗黄萎病est‑ssr分子标记及其制备方法 |
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CN201410230026.5A CN104404035B (zh) | 2014-05-28 | 2014-05-28 | 一种棉花抗黄萎病est‑ssr分子标记及其制备方法 |
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CN104404035A CN104404035A (zh) | 2015-03-11 |
CN104404035B true CN104404035B (zh) | 2017-09-26 |
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CN201410230026.5A Active CN104404035B (zh) | 2014-05-28 | 2014-05-28 | 一种棉花抗黄萎病est‑ssr分子标记及其制备方法 |
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Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105713976B (zh) * | 2016-03-29 | 2019-01-11 | 江苏省农业科学院 | 能提高陆地棉黄萎病抗性的海岛棉染色体片段及分子标记 |
CN112868522A (zh) * | 2021-01-19 | 2021-06-01 | 山西农业大学 | 一种远缘杂交创制棉花新种质的方法 |
CN114634993B (zh) * | 2022-04-27 | 2023-03-14 | 南通大学 | 一种基于转录组和蛋白组联合分析的棉花耐盐基因发现方法及其应用 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103160506A (zh) * | 2013-03-14 | 2013-06-19 | 南通大学 | 棉花耐盐est-ssr分子标记 |
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- 2014-05-28 CN CN201410230026.5A patent/CN104404035B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103160506A (zh) * | 2013-03-14 | 2013-06-19 | 南通大学 | 棉花耐盐est-ssr分子标记 |
Non-Patent Citations (2)
Title |
---|
Development of EST-SSR markers related to disease resistance and their application ingenetic diversity and evolution analysis in Gossypium;B.H. Wang et al;《Genetics and Molecular Research》;20150909;第14卷(第3期);10630-10644 * |
棉花黄萎病抗性的分子标记研究;孔祥瑞;《中国优秀硕士学位论文全文数据库 农业科技辑》;20111015;摘要,正文第12、15-18、31-34页 * |
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Application publication date: 20150311 Assignee: Nantong Pharmaceutical Co.,Ltd. Assignor: Center for technology transfer, Nantong University Contract record no.: X2021980016780 Denomination of invention: Cotton Verticillium wilt resistant EST-SSR molecular marker and preparation method thereof Granted publication date: 20170926 License type: Common License Record date: 20211229 |
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Application publication date: 20150311 Assignee: Nantong Yifan Biotechnology Co.,Ltd. Assignor: Center for technology transfer, Nantong University Contract record no.: X2022320000354 Denomination of invention: EST-SSR Molecular Marker of Cotton Resistance to Verticillium Wilt and Its Preparation Method Granted publication date: 20170926 License type: Common License Record date: 20221210 |
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Address after: 226001 No.9, Siyuan Road, Chongchuan District, Nantong City, Jiangsu Province Patentee after: Nantong University Technology Transfer Center Co.,Ltd. Address before: 226019 No.205, building 6, Nantong University, No.9, Siyuan Road, Nantong City, Jiangsu Province Patentee before: Center for technology transfer, Nantong University |
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Application publication date: 20150311 Assignee: NANTONG HUIRAN BIOLOGICAL SCIENCE & TECHNOLOGY CO.,LTD. Assignor: Nantong University Technology Transfer Center Co.,Ltd. Contract record no.: X2023980053913 Denomination of invention: A Cotton Resistance to Verticillium Wilt EST-SSR Molecular Marker and Its Preparation Method Granted publication date: 20170926 License type: Common License Record date: 20231225 |
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