CN104384010A - Method for purifying resting eggs of seawater branchionus plicatilis - Google Patents

Abstract

The invention provides a method for purifying resting eggs of seawater branchionus plicatilis. The method comprises the steps of feeding fresh water into a container, and then feeding a sodium hypochlorite disinfectant to enable available chlorine in the solution to reach 10-20PPm; putting the resting egg raw material of the seawater branchionus plicatilis into the container, mixing, and enabling the resting eggs of the branchionus plicatilis to be fully hydrated; stirring light impurities floating on the upper layer water surface, and then standing still for 30-60 minutes; transferring lower-layer heavy impurities, containing the resting eggs of the branchionus plicatilis and silt, in a mesh bag into a container, wherein the container is filled with high-concentration brine, and the volume of the brine is 10-15 times that of the transferred material; stirring the resting eggs of the branchionus plicatilis floating on the upper layer water surface to enable the silt heavy impurity mixed in the resting eggs of the branchionus plicatilis to be fully separated, and standing still; removing the heavy impurities at the lower layer under the resting eggs of the branchionus plicatilis at the upper layer, and using the mesh bag to receive the resting eggs of the branchionus plicatilis at the upper layer. After the method is adopted, the purification rate of the resting eggs of the seawater branchionus plicatilis reaches up to more than 98%.

Description

A kind of purification process of seawater brachionus plicatilis resting egg

Technical field

The present invention relates to technical field of aquaculture.In particular to a kind of purification process of seawater brachionus plicatilis resting egg.

Background technology

Along with developing rapidly of culture fishery, water rotifer has become the indispensable opening fresh food of aquatic fry growing.Current domestic aquatic products producer will utilize wild wheel animalcule and using water rotifer resting egg as provenance, after hatching, more manually cultivate, and is supplied to aquatic seedling and makes open-mouthed bait.In China, particularly northern area, owing to affecting by temperature, only just has wild wheel animalcule in summer, autumn, and the wheel animalcule quality of gathering and supply instability, affect seedling percent.And using water rotifer resting egg as provenance, after hatching, carry out artificial mode of cultivating again, Most users uses the Resting Eggs of Rotifers In The Water after gathering directly to hatch, not purified, sterilization, wheel animalcule provenance after such hatching contains a large amount of impurity and bacterium, not only have impact on the cultivation of wheel animalcule but also have impact on the survival rate of aquatic seedling.

Summary of the invention

The invention provides a kind of purification process of seawater brachionus plicatilis resting egg, its technology provides healthy wheel animalcule provenance by removing impurity in raw material resting egg and bacterium for aquatic seedling.

In order to achieve the above object, technical scheme of the present invention is also sterilized and remove heavy seeds two steps in seawater brachionus plicatilis resting egg raw material to complete by the light impurity removed in seawater brachionus plicatilis resting egg raw material.

Concrete technical scheme of the present invention is as follows:

A purification process for seawater brachionus plicatilis resting egg, be according to the hydration of seawater brachionus plicatilis resting egg after proportion principle process, step is as follows:

1) remove the light impurity in seawater brachionus plicatilis resting egg raw material and sterilize;

2) heavy seeds in seawater brachionus plicatilis resting egg raw material is removed.

The method that light impurity in concrete removal seawater brachionus plicatilis resting egg raw material is also sterilized is:

1): in transparent container, add fresh water, its volume is 10 ~ 15 times of seawater brachionus plicatilis resting egg material quantity, then adds sodium hypochlorite, makes the effective chlorine of solution reach 10 ~ 20PPm;

2) seawater brachionus plicatilis resting egg raw material is put into step 1) container, stir, make the abundant hydration of Resting Eggs of Rotifers In The Water; After layering, stir the light impurity swimming in topmost surface, the Resting Eggs of Rotifers In The Water be mingled in light impurity is separated, then static 30 ~ 60 minutes;

3) lower floor is the heavy seeds containing Resting Eggs of Rotifers In The Water and silt, receives lower floor's material with 250 ~ 300 mesh sieve tulle bags.

In concrete removal seawater brachionus plicatilis resting egg raw material, the method for heavy seeds is:

1) after the Resting Eggs of Rotifers In The Water in mesh bag and silt heavy seeds being drained the water, proceed to fill height bittern (salinity 20% ~ saturated bittern) container in, bittern volume is proceed to material 10 ~ 15 times of volume, stirs, Resting Eggs of Rotifers In The Water is fully dewatered;

2) after layering, then the Resting Eggs of Rotifers In The Water swimming in topmost surface is stirred, to make the heavy seeds of the silt be mingled in Resting Eggs of Rotifers In The Water fully be separated, then static 30 ~ 60 minutes;

3) upper strata is Resting Eggs of Rotifers In The Water, the heavy seeds of lower floor of draining, and receives the Resting Eggs of Rotifers In The Water on upper strata with 250 ~ 300 mesh sieve tulle bags.

Effect of the present invention is: by the two-step pretreatment to seawater brachionus plicatilis resting egg raw material, eliminate light impurity and the heavy seeds such as mud, sand such as grass bits, ghost in raw material, the purifying rate of seawater brachionus plicatilis resting egg is made to reach more than 98%, simultaneously, eliminate resting egg surface with harmful bacteria, can be the wheel animalcule provenance that aquatic seedling provides healthy.

Detailed description of the invention

Below in conjunction with specific embodiment, the present invention is described in further detail:

The process of embodiment 1 light impurity and sterilization

In transparent conical vessel, add fresh water, its volume is 10 ~ 15 times of seawater brachionus plicatilis resting egg material quantity, then adds sodium hypochlorite, make the effective chlorine density of solution be respectively 5,10,15,20,25PPm.The same a collection of seawater brachionus plicatilis resting egg raw material of gathering is put into respectively the conical vessel of different effective chlorine density, stir, make the abundant hydration of Resting Eggs of Rotifers In The Water.After layering, more slowly stir the light impurity swimming in topmost surface, to make the Resting Eggs of Rotifers In The Water be mingled in light impurity fully be separated, improve the productive rate of Resting Eggs of Rotifers In The Water.Then static 30 ~ 60 minutes, lower floor was the heavy seeds such as Resting Eggs of Rotifers In The Water and silt, and upper strata is that Resting Eggs of Rotifers In The Water ghost and grass bits wait light impurity.Receive the heavy seeds such as the Resting Eggs of Rotifers In The Water of lower floors and silt with 250 ~ 300 mesh sieve tulle bags, Resting Eggs of Rotifers In The Water ghost and the light impurity such as grass bits grade on upper strata are drained.Resting egg after different effective chlorine density of learning from else's experience sterilization carries out Bacteria Culture, then carries out qualitative analysis.Resting egg after the process of 5PPm effective chlorine contains more bacterium colony, and after the process of 10PPm effective chlorine, resting egg only finds individual colonies, through 15,20, resting egg after the process of 25PPm effective chlorine all do not find bacterium colony.Get the resting egg after the sterilization of different effective chlorine density, after going diapause under the same conditions, detect its main index-incubation rate, result is as follows:

Resting egg incubation rate after different effective chlorine density sterilization

Batch	5PPm	10PPm	15PPm	20PPm	25PPm
						1	91.2％	92.1％	91.8％	90.5％	87.6％
2	90.7％	91.5％	90.3％	91.1％	85.8％
						3	91.5％	91.3％	92.3％	91.0％	87.1％

On average

91.1％

91.6％

91.5％

90.9％

86.8％

Find out from upper table: after the sterilization of 25PPm effective chlorine density, the incubation rate of resting egg reduces, so the concentration adding sodium hypochlorite in fresh water controls between 10 ~ 20PPm.

The process of embodiment 2 heavy seeds

After the heavy seeds such as the Resting Eggs of Rotifers In The Water received in mesh bag and silt are drained the water, proceed to respectively fill salinity 15%, 20%, 25% and saturated bittern conical vessel in, bittern volume is 10 ~ 15 times that proceed to material volume, stirs, Resting Eggs of Rotifers In The Water is fully dewatered.Stop stirring, after layering, more slowly stir the Resting Eggs of Rotifers In The Water swimming in topmost surface, be fully separated to make the being mingled in heavy seeds such as the silt in Resting Eggs of Rotifers In The Water, improve the purifying rate of Resting Eggs of Rotifers In The Water.Then static 30 ~ 60 minutes, its point of layer status is observed.In the container filling salinity 15% bittern, after static 60 minutes, still have partial sleep ovum to be suspended in bittern, show that resting egg does not dewater completely in the bittern of 15% salinity, it is larger than escheat.And in the conical vessel filling salinity 20%, 25% and saturated bittern, static 30 ~ 60 minutes, in bittern, there is no the resting egg suspended, layering is thorough.Lower floor is the heavy seeds such as silt, and upper strata is Resting Eggs of Rotifers In The Water.To drain the heavy seeds of lower floor, receive the Resting Eggs of Rotifers In The Water on upper stratas with 250 ~ 300 mesh sieve tulle bags.

The purifying rate of this Resting Eggs of Rotifers In The Water reaches more than 98%.

Claims (3)

1. the purification process of a seawater brachionus plicatilis resting egg; It is characterized in that step is as follows:

1) remove the light impurity in seawater brachionus plicatilis resting egg raw material and sterilize;

2) heavy seeds in seawater brachionus plicatilis resting egg raw material is removed.

2. the method for claim 1, is characterized in that the method that the light impurity in removal seawater brachionus plicatilis resting egg raw material is also sterilized is:

1): in transparent container, add fresh water, its volume is 10 ~ 15 times of seawater brachionus plicatilis resting egg material quantity, then adds sodium hypochlorite, makes the effective chlorine of solution reach 10 ~ 20PPm;

2) seawater brachionus plicatilis resting egg raw material is put into step 1) container, stir, make the abundant hydration of Resting Eggs of Rotifers In The Water; After layering, stir the light impurity swimming in topmost surface, the Resting Eggs of Rotifers In The Water be mingled in light impurity is separated, then static 30 ~ 60 minutes;

3) lower floor is the heavy seeds containing Resting Eggs of Rotifers In The Water and silt, receives lower floor's material with 250 ~ 300 mesh sieve tulle bags.

3. the method for claim 1, is characterized in that the method removing heavy seeds in seawater brachionus plicatilis resting egg raw material is:

1) after the Resting Eggs of Rotifers In The Water in mesh bag and silt heavy seeds being drained the water, proceed to fill height bittern (salinity 20% ~ saturated bittern) container in, bittern volume is proceed to material 10 ~ 15 times of volume, stirs, Resting Eggs of Rotifers In The Water is fully dewatered;

2) after layering, then the Resting Eggs of Rotifers In The Water swimming in topmost surface is stirred, to make the heavy seeds of the silt be mingled in Resting Eggs of Rotifers In The Water fully be separated, then static 30 ~ 60 minutes;

3) upper strata is Resting Eggs of Rotifers In The Water, the heavy seeds of lower floor of draining, and receives the Resting Eggs of Rotifers In The Water on upper strata with 250 ~ 300 mesh sieve tulle bags.