CN104381601A - EM (Efficient Microorganisms) raw liquor for preventing and treating diseases of animals and plants and improving immunity, and preparation method thereof - Google Patents
EM (Efficient Microorganisms) raw liquor for preventing and treating diseases of animals and plants and improving immunity, and preparation method thereof Download PDFInfo
- Publication number
- CN104381601A CN104381601A CN201410579650.6A CN201410579650A CN104381601A CN 104381601 A CN104381601 A CN 104381601A CN 201410579650 A CN201410579650 A CN 201410579650A CN 104381601 A CN104381601 A CN 104381601A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- anaerobic fermentation
- degree
- temperature
- agitator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Fodder In General (AREA)
Abstract
The invention relates to an EM (Efficient Microorganisms) raw liquor for preventing and treating diseases of animals and plants and improving immunity, and a preparation method for the liquor. The preparation method of the raw liquor comprises the following steps: step (1), compounding raw materials of a culture medium in a compounding ratio; step (2), performing aerobic fermentation; step (3), performing anaerobic fermentation; step (4), performing stirring aerobic fermentation; step (5) performing stirring anaerobic fermentation. The prepared Chinese herbal medicine EM raw liquor can improve the feed utilization rate, when the raw liquor is used for feeding the animals, the immunity of the animals can be obviously improved, the attack rate of enteritis of cubs is reduced, the yield is increased, the meat quality is improved, the storage life is prolonged, and fetor from a breeding field is effectively restrained; when the raw liquor is used for cultivating crops, the structure of soil can be improved, organic compounds which are difficult to decompose in the soil are decomposed, diseases and insect pests are prevented, fruits are promoted to become ripe, and the storage life of the crops is prolonged; when the raw liquor is used in the environment, the organic compounds in water of polluted rivers and lakes can be decomposed, the microbial population in the water is improved, and water quality is purified.
Description
Technical field
The present invention relates to Chinese herbal medicine and microbial preparation field, particularly relate to and a kind ofly animals and plants are prevented and treated and improves efficient EM stoste and the method thereof of immunity.
Background technology
Along with the progress of science and technology and the raising of people's living standard, pursue the free of contamination healthy food of fully natural green and become a kind of fashion, but due to agricultural chemicals, chemical fertilizer, a large amount of uses of antibiotic and growth hormone, make the deterioration of the ecological environment, the quality .EM having a strong impact on agricultural production is " effective microbe " english abbreviation of (Efective Microorganisms), it can make photosynthetic bacteria, lactic acid bacteria, saccharomycete, bacillus, acetic acid bacteria, Bifidobacterium, in the large quasi-microorganism of actinomyces seven 10 belong to 80 kinds of beneficial microbe coexistence and co-prosperities, the beginning of the nineties at the end of the eighties, EM bacterium is Japanese, Thailand, Brazil, the U.S., Indonesia, the states such as Sri Lanka are widely used in agricultural, cultivation, plantation, the fields such as environmental protection, achieve obvious economic benefit and ecological benefits.
The EM technical products of existing market, its production technology is simple, extensive, under production efficiency, viable count is low, and retention cycle is short, and it is in livestock-raising, aquaculture, plantation and water correction aspect have little effect, and exaggerate, especially prevent and cure diseases animals and plants, improve animals and plants immunity, animals and plants increasing both production and income aspect does not almost have effect.
Prior art can not meet the needs of people, and for making up prior art deficiency, the present invention aims to provide and a kind ofly prevents and treats animals and plants and improve efficient EM stoste and the method thereof of immunity; It makes part Chinese herbal medicine be decomposed into the secondary metabolite of more easily absorption and better effects if by probio by adding the usefulness that a kind of Chinese herb feed additive improves EM stoste in the culture medium of fermentation EM, thus the usefulness of EM stoste is increased dramatically.
For achieving the above object, the present invention by the following technical solutions: a kind ofly prevent and treat animals and plants and improve efficient EM stoste and the method thereof of immunity, its preparation method comprises following step, step one: culture medium raw material and proportioning preparation; Step 2: aerobic fermentation; Step 3: anaerobic fermentation; Step 4: be stirred aerobe fermentation; Step 5: stir anaerobic fermentation;
Wherein in step one: culture medium comprises the raw material of following weight proportion: salt 1.5-2 part, molasses 10-15 part, glucose 20-30 part, yeast extract 10-20 part, Chinese herb feed additive 2-4 part, water 1500-1600 part; Described Chinese herb feed additive comprises pine needle, fructus hordei germinatus, purslane, poplar flower, the extract of the mixtures such as STEVIA REBAUDIANA, Radix Codonopsis, the Radix Astragali;
In step 2: culture medium and water are loaded in fermentation tank in proportion, temperature is controlled at about 37 degree after high-temperature sterilization, add EM bacterium bacterial classification 15-20 part, mix and blend, control throughput at 1.0-1.5vvm, speed of agitator is 200-300rpm, and temperature is 32-39 degree, continues aerobic fermentation 10-12 hour;
In step 3: close ventilation, carry out anaerobic fermentation, speed of agitator is 100-150rpm, and temperature controls at 32-39 degree, continue anaerobic fermentation 12-14 hour;
In step 4: control throughput at 1.0-1.5vvm, speed of agitator is 200-300rpm, and temperature is 32-39 degree, continues aerobic fermentation 20-24 hour;
In step 5: close ventilation, carry out anaerobic fermentation, speed of agitator is 100-150rpm, and temperature controls at 32-39 degree, continue anaerobic fermentation 30-36 hour and get product.
Compared with prior art, the invention has the beneficial effects as follows: the Chinese herbal medicine EM bacterium stoste prepared by the present invention can improve efficiency of feed utilization, obviously animal immunizing power can be improved for animal feeding, reduce the incidence of disease of cub enteritis, increase yield, improve meat, extend the pot-life, and effectively suppress the stench of plant; Can improve soil texture for arviculture, decompose the difficult organic matter decomposed in soil, pre-preventing disease and pest, promotes fruit maturation, extends the crops pot-life; The organic matter in contaminated rivers and lakes water can be decomposed for environment aspect, improve microbial population in water, purify water.
Summary of the invention
Detailed description of the invention
Be clearly and completely described to the technical scheme in the embodiment of the present invention below, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to the scope of protection of the invention.
In the embodiment of the present invention, a kind ofly prevent and treat animals and plants and improve efficient EM stoste and the method thereof of immunity, its preparation method comprises following step, step one: culture medium raw material and proportioning preparation; Step 2: aerobic fermentation; Step 3: anaerobic fermentation; Step 4: be stirred aerobe fermentation; Step 5: stir anaerobic fermentation;
Wherein in step one: culture medium comprises the raw material of following weight proportion: salt 1.5-2 part, molasses 10-15 part, glucose 20-30 part, yeast extract 10-20 part, Chinese herb feed additive 2-4 part, water 1500-1600 part; Described Chinese herb feed additive comprises pine needle, fructus hordei germinatus, purslane, poplar flower, the extract of the mixtures such as STEVIA REBAUDIANA, Radix Codonopsis, the Radix Astragali;
In step 2: culture medium and water are loaded in fermentation tank in proportion, temperature is controlled at about 37 degree after high-temperature sterilization, add EM bacterium bacterial classification 15-20 part, mix and blend, control throughput at 1.0-1.5vvm, speed of agitator is 200-300rpm, and temperature is 32-39 degree, continues aerobic fermentation 10-12 hour;
In step 3: close ventilation, carry out anaerobic fermentation, speed of agitator is 100-150rpm, and temperature controls at 32-39 degree, continue anaerobic fermentation 12-14 hour;
In step 4: control throughput at 1.0-1.5vvm, speed of agitator is 200-300rpm, and temperature is 32-39 degree, continues aerobic fermentation 20-24 hour;
In step 5: close ventilation, carry out anaerobic fermentation, speed of agitator is 100-150rpm, and temperature controls at 32-39 degree, continue anaerobic fermentation 30-36 hour and get product.
The culture medium of embodiment one: EM strain fermentation comprises following raw material: salt 1.6kg, molasses 15kg, glucose 25kg, yeast extract 10kg, Chinese herb feed additive 2kg, water 1500kg.
Culture medium and water are loaded in proportion in fermentation tank, temperature controlled at about 37 degree after high-temperature sterilization, add EM bacterium bacterial classification 20kg, mix and blend, control throughput at 1.5vvm, speed of agitator is 250rpm, and temperature is 37 degree, continues aerobic fermentation 12 hours,
Close ventilation, carry out anaerobic fermentation, speed of agitator is 100rpm, and temperature controls at 36 degree, continues anaerobic fermentation 12 hours
Control throughput at 1.0vvm, speed of agitator is 250rpm, and temperature is 37 degree, continues aerobic fermentation 20 hours.
Close ventilation, carry out anaerobic fermentation, speed of agitator is 100rpm, and temperature controls at 36 degree, continues anaerobic fermentation and gets product for 36 hours.
The culture medium of embodiment 2:EM strain fermentation comprises following raw material: salt 1.6kg, molasses 10kg, glucose 30kg, yeast extract 11kg, Chinese herb feed additive 3kg, water 1500kg.
Culture medium and water are loaded in proportion in fermentation tank, temperature controlled at about 37 degree after high-temperature sterilization, add EM bacterium bacterial classification 20kg, mix and blend, control throughput at 1.5vvm, speed of agitator is 300rpm, and temperature is 37 degree, continues aerobic fermentation 10 hours;
Close ventilation, carry out anaerobic fermentation, speed of agitator is 100rpm, and temperature controls at 36 degree, continues anaerobic fermentation 12 hours;
Control throughput at 1.0vvm, speed of agitator is 200rpm, and temperature is 37 degree, continues aerobic fermentation 24 hours;
Close ventilation, carry out anaerobic fermentation, speed of agitator is 100rpm, and temperature controls at 36 degree, continues anaerobic fermentation and gets product for 32 hours.
The culture medium of embodiment three: EM strain fermentation comprises following raw material: salt 1.5kg, molasses 12kg, glucose 28kg, yeast extract 15kg, Chinese herb feed additive 2kg, water 1500kg.
Culture medium and water are loaded in proportion in fermentation tank, temperature controlled at about 37 degree after high-temperature sterilization, add EM bacterium bacterial classification 20kg, mix and blend, control throughput at 1.5vvm, speed of agitator is 250rpm, and temperature is 37 degree, continues aerobic fermentation 12 hours;
Close ventilation, carry out anaerobic fermentation, speed of agitator is 150rpm, and temperature controls at 36 degree, continues anaerobic fermentation 12 hours;
Control throughput at 1.0vvm, speed of agitator is 200rpm, and temperature is 37 degree, continues aerobic fermentation 22 hours;
Close ventilation, carry out anaerobic fermentation, speed of agitator is 100rpm, and temperature controls at 36 degree, continues anaerobic fermentation and gets product for 35 hours.
EXPERIMENTAL EXAMPLE 1,
The use of Chinese herbal medicine fermentation EM stoste on breeding layer chicken:
Get ABC tri-groups of laying hens and A1B1C1 tri-groups contrast respectively, A and A1 chicken age is 42 week age (each 12500), B and B1 is 80 week age (each 12500), C and C1 group 102 week age (each 15000),
The chicken feed of ABC tri-groups is mixed feeding after 1 liter of EM stoste every day, A1B1C1 tri-groups then feeding common chicken feed, detected after 8 weeks and find, the relative A1B1C1 of ABC tri-groups tri-groups of laying rate all rise 1-2%, dirty egg rate of broken eggs declines 12%, egg size increases by 15%, and chicken manure reduces 20%, and laying period continues to increase.
EXPERIMENTAL EXAMPLE 2,
The effect of Chinese herbal medicine fermentation EM stoste in young chicken intestinal diorder prevention:
Get G1 respectively, G2, G3, each 24 the young chickens of G4 tetra-groups, G1 group echo is negative control group, G2 group echo is positive controls, adds the Chinese herbal medicine fermentation EM feeding of 0.1% in G3 group feed every day, adds the Chinese herbal medicine fermentation EM feeding of 0.5% in G4 group group feed every day, feeding is after 4 weeks again, G2, G3, G4 group is inoculated respectively to the C.Perfringens bacterium easily causing enteritis, before continuing afterwards, feeding patterns is raised, after two weeks, case fatality rate is as follows respectively, and G1 group is 0%, G2 group 41.7%, G3 group is 33.3%, G4 group is 16.7%.As can be seen here along with the increase of Chinese herbal medicine EM addition in feed obviously can improve the enteritis case fatality rate of young chicken.
Experiment conclusion proves that Chinese herbal medicine fermentation EM bacterium stoste is used for breeding layer chicken and can improves egg production, improve efficiency of feed utilization, reduce young chicken case fatality rate, improve the resistance of chicken, and can reducing or not use antibiotic, is a kind of agricultural biologic product of ecological ideas.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or essential characteristic, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
The above; be only preferred embodiment of the present invention; not in order to limit the present invention, every above embodiment is done according to technical spirit of the present invention any trickle amendment, equivalently replace and improve, within the protection domain that all should be included in technical solution of the present invention.
Claims (1)
1. prevent and treat animals and plants and improve efficient EM stoste and the method thereof of immunity, its preparation method comprises following step, step one: culture medium raw material and proportioning preparation; Step 2: aerobic fermentation; Step 3: anaerobic fermentation; Step 4: be stirred aerobe fermentation; Step 5: stir anaerobic fermentation; It is characterized in that:
S1. culture medium comprises the raw material of following weight proportion: salt 1.5-2 part, molasses 10-15 part, glucose 20-30 part, yeast extract 10-20 part, Chinese herb feed additive 2-4 part, water 1500-1600 part; Described Chinese herb feed additive comprises pine needle, fructus hordei germinatus, purslane, poplar flower, the extract of the mixtures such as STEVIA REBAUDIANA, Radix Codonopsis, the Radix Astragali;
S2. culture medium and water are loaded in fermentation tank in proportion, temperature is controlled at about 37 degree after high-temperature sterilization, add EM bacterium bacterial classification 15-20 part, mix and blend, control throughput at 1.0-1.5vvm, speed of agitator is 200-300rpm, temperature is 32-39 degree, continues aerobic fermentation 10-12 hour;
S3. close ventilation, carry out anaerobic fermentation, speed of agitator is 100-150rpm, and temperature controls at 32-39 degree, continues anaerobic fermentation 12-14 hour;
S4. control throughput at 1.0-1.5vvm, speed of agitator is 200-300rpm, and temperature is 32-39 degree, continues aerobic fermentation 20-24 hour;
S5. close ventilation, carry out anaerobic fermentation, speed of agitator is 100-150rpm, and temperature controls at 32-39 degree, continues anaerobic fermentation 30-36 hour and gets product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410579650.6A CN104381601A (en) | 2014-10-16 | 2014-10-16 | EM (Efficient Microorganisms) raw liquor for preventing and treating diseases of animals and plants and improving immunity, and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410579650.6A CN104381601A (en) | 2014-10-16 | 2014-10-16 | EM (Efficient Microorganisms) raw liquor for preventing and treating diseases of animals and plants and improving immunity, and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104381601A true CN104381601A (en) | 2015-03-04 |
Family
ID=52599651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410579650.6A Pending CN104381601A (en) | 2014-10-16 | 2014-10-16 | EM (Efficient Microorganisms) raw liquor for preventing and treating diseases of animals and plants and improving immunity, and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104381601A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905050A (en) * | 2015-05-28 | 2015-09-16 | 青岛农业大学 | High-value utilization method of plant hot-pressing cake meal for preventing and treating aquatic animal diseases |
| CN109512934A (en) * | 2017-09-20 | 2019-03-26 | 扬中牧乐药业有限公司 | A kind of oral solution and preparation method thereof for treating swine enteritis |
| CN109588558A (en) * | 2019-01-30 | 2019-04-09 | 永胜腾飞家禽养殖有限公司 | A kind of livestock birds health cultivation feed addictive, preparation method and applications |
| CN109601717A (en) * | 2019-01-30 | 2019-04-12 | 永胜腾飞家禽养殖有限公司 | A kind of method of livestock birds health cultivation |
| CN114403286A (en) * | 2022-01-12 | 2022-04-29 | 淮安市银杏林生态农业发展有限公司 | Livestock EM nutrient solution containing ginkgo leaf extract and preparation method and application thereof |
-
2014
- 2014-10-16 CN CN201410579650.6A patent/CN104381601A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905050A (en) * | 2015-05-28 | 2015-09-16 | 青岛农业大学 | High-value utilization method of plant hot-pressing cake meal for preventing and treating aquatic animal diseases |
| CN109512934A (en) * | 2017-09-20 | 2019-03-26 | 扬中牧乐药业有限公司 | A kind of oral solution and preparation method thereof for treating swine enteritis |
| CN109588558A (en) * | 2019-01-30 | 2019-04-09 | 永胜腾飞家禽养殖有限公司 | A kind of livestock birds health cultivation feed addictive, preparation method and applications |
| CN109601717A (en) * | 2019-01-30 | 2019-04-12 | 永胜腾飞家禽养殖有限公司 | A kind of method of livestock birds health cultivation |
| CN114403286A (en) * | 2022-01-12 | 2022-04-29 | 淮安市银杏林生态农业发展有限公司 | Livestock EM nutrient solution containing ginkgo leaf extract and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5690401B2 (en) | Organic fermentation composition and method for producing the same | |
| CN107129953B (en) | Microbial additive for treating cattle manure and preparation method and application thereof | |
| CN104261911A (en) | Method for preparing organic fertilizer by using plant residues as raw material | |
| CN104177139A (en) | Chicken manure organic fertilizer for microbial fermentation | |
| CN104381601A (en) | EM (Efficient Microorganisms) raw liquor for preventing and treating diseases of animals and plants and improving immunity, and preparation method thereof | |
| CN104261919A (en) | Process for producing efficient bioorganic fertilizers by resource utilization of dairy cow manure | |
| KR102222811B1 (en) | Additive composition for fertilizer and animal feed, and manufacturing mehtod thereof | |
| CN104250119B (en) | A kind of method that fermentation waste water and agricultural wastes produce biological organic fertilizer | |
| CN104472865A (en) | Method for preparing silage | |
| CN105967947A (en) | Ferment biological bacterial fertilizer using processing by-products of preserved fruits of apples as main raw material | |
| CN103274831A (en) | Method for preparing traditional Chinese medicine microbial fertilizer of spinach | |
| CN105060944A (en) | Method for preparing egg white fertilizer by fish soluble | |
| CN107473861A (en) | A kind of special complex fertilizer for alleviating citrus yellow and preparation method thereof | |
| CN103739410B (en) | A kind of making method of organic watermelons continuous cropping high yield compound bacterial fertilizer | |
| CN107118060A (en) | Biological organic fertilizer and preparation method thereof | |
| CN105272556A (en) | Aquaculture biofertilizer and preparation method thereof | |
| CN105565918A (en) | Plant nutrition package green organic fertilizer | |
| CN103965915A (en) | Crop nutrition agent and preparation method thereof | |
| CN113511943A (en) | Formula and preparation method for preparing efficient biological organic fertilizer from organic materials | |
| CN101288359A (en) | Method for breaking hard seed dormancy of alfalfa seeds | |
| CN102633560B (en) | Multiple-effect biological insecticide-fertilizer and preparation method thereof | |
| CN108218589A (en) | A kind of lotus rhizome long-acting environment friendly organic fertilizer and preparation method thereof | |
| CN104496575A (en) | Organic fertilizer for resisting continuous cropping obstacles of chuzhou chrysanthemum and preparation method of organic fertilizer | |
| CN104431315A (en) | Organic enzyme biological feed | |
| CN104072298A (en) | High-safety high-efficiency environment-friendly biological fish fertilizer and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20150304 |