CN104381343A - Water-soluble compound antibacterial agent and preparation method thereof - Google Patents
Water-soluble compound antibacterial agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a water-soluble compound antibacterial agent and its preparation method. Antibacterial active ingredients of the water-soluble compound antibacterial agent are composed of a garlic extract product, a cordate houttuynia extract product, a tarragon extract product, a chrysanthemum extract product, a folium eriobotryae extract product, a lophatherum gracile extract product and a folium cortex eucommiae extract product. Garlic, cordate houttuynia, tarragon, chrysanthemum, folium eriobotryae, lophatherum gracile and folium cortex eucommiae which have different degrees of inhibiting effects on bacteria, mould and yeast are used as raw materials to be mixed so as to prepare the antibacterial agent. Thus, antimicrobial spectrum of the antibacterial agent is broadened effectively, and the defect that current plant antibacterial agents are prepared by using a single plant as a raw material and have poor broad-spectrum antibacterial effect is overcome. The antibacterial agent can inhibit a wide range of bacteria and has better effects. The invention also discloses a preparation method of the water-soluble compound antibacterial agent.
Description
Technical field
Technical field: the present invention relates to a kind of bacteriostatic agent, especially relates to a kind of water-soluble compound type bacteriostat, the invention still further relates to the preparation method of this water-soluble compound type bacteriostat.
Background technology
In extended day articles for use storage life, ensure that in the process of commodity quality, commodity bacteriostatic agent has very important effect.The main component of traditional commodity bacteriostatic agent is synthetic, and this synthesis bacteriostatic agent exists the problem of toxicity, is limited use gradually in many countries, and therefore commodity additive has been formed toward nontoxic, safety, the international trend of natural future development.
Allicin contained by garlic has stronger antibacterial and anti-inflammation functions, all has suppression or killing action to multiple coccus, bacillus, fungi, virus etc.Allicin significantly suppresses or killing action as staphylococcus, meningitis, Diplococcus pneumopniae, streptococcus and diphtheria, dysentery, Escherichia coli, typhoid fever, paratyphoid, pertussis, tubercle bacillus and comma bacillus etc. have various pathogens.The antibacterial principle of allicin inhibits the Growth and reproduction of bacterium because the oxygen atom in allicin molecule and the bacterial growth sulfydryl of breeding in necessary cysteine molecule combines.People's nineteen eighty-twos such as Saleen are studied and show, garlic P.E to staphylococcus aureus salmonella, hay bacillus, the bacteriums such as Escherichia coli and aspergillus oryzae, the inhibitory action of the moulds such as aspergillus niger is the strongest, and to brewer's yeast, the yeast such as Candida also have certain inhibitory action.
The main antibiotic effective ingredient decanoyl acetaldehyde of cordate houttuynia is to various bacteria, acid-fast bacilli and fungi etc. all have obvious antibacterial action, but this kind of compound property instability. its sodium hydrogensulfite addition product then stable in properties and retain its antibacterial activity. decanoy acetaldehyde all has obvious bacteriostasis to multiple Grain-positive and negative bacteria in vitro, with staphylococcus aureus and penicillin resistant strain thereof, Diplococcus pneumopniae, alpha streptococcus, Bacillus influenzae is comparatively responsive, micrococcus catarrhalis, typhoid bacillus takes second place, and Escherichia coli, Pseudomonas aeruginosa and shigella dysenteriae are very inresponsive. for the tubercle bacillus affection of mouse, decanoy acetaldehyde can obviously extend its life span. in addition, to Candida albicans, Cryptococcus neoformans, sporothrix, aspergillus, painted mould, red mentagrophytes, trichophyton concentricum, Microsporum gypseum, Microsporum ferrugineum, shark tinea bacterium etc. also has obvious inhibitory action.
Tarragon is containing volatile oil, for multicomponent mixture, through being separated having of qualification: terpinenol-4(Terpinenol-4), β-caryophyllene (β-Caryophyllene), artemisol (Artemisia alcohol), linalool (Linalool), camphor (Camphorae), borneol (borneol Borneol), cineole (Cineol, Eucalyptol) and phellandrene (Phellandrene), Cadinene (Cadinene), thujanol (Thujyl alcohol) etc.
Antibacterial action
Tarragon all has antibacterial action to 10 kinds of Gram-positive aerophils such as bacillus anthracis, Alpha-hemolytic streptococcus, B-Streptococcus hemolyticus, diphtheria bacillus, bacillus pseudodiphthericus, lung diplococcoid, staphylococcus aureus, Staphylococcus citreus, white Portugal Portugal coccus, hay bacilluses in vitro.Blumea oil has bacteriostasis to Diplococcus pneumopniae, staphylococcus aureus, staphylococcus albus, alpha streptococcus, Escherichia coli, typhoid bacillus, paratyphosum Bacterium, shigella flexneri etc.With mugwort leaf, moxa stick or moxa sootiness, can be used for indoor sterilization, with rhizoma atractylodis or with calamus and realgar or mix cigarette weight with rhizoma atractylodis, realgar, the root of Dahurain angelica etc., staphylococcus aureus, beta hemolytic streptococcus, Escherichia coli, proteus, diphtheria bacillus, typhoid fever and paratyphosum Bacterium, Pseudomonas aeruginosa, hay bacillus, Bacillus alcaligenes and tubercle bacillus are all killed or inhibitory action.
Antifungic action
Tarragon decocting liquid is to the bacteriostasis of psoriasis fungi the faintest (compared with the decocting liquid such as the coptis, the root of large-flowered skullcap), the trichophyton when 15% concentration, start in suppressing, during 30% concentration except acrothesium floccosum, sufficient sole of the foot trichophyta and Candida albicans are still grown, other is all stasi such as oidium schoenleinii, oidium schoenleinii Mongolia mutation, dog mountain brood cell's tinea bacterium, Trichophyton concentricum, Trichophyton rubrum, rust trichophyta, trichophyton.Cao Honglie etc. also prove the water logging agent (1:4) of tarragon, in vitro to trichophyton, the dermatophyte such as oidium schoenleinii, Microsporon audouini, ulotrichy microspore tinea bacterium, epidermophyton rubrum, star nocardia all has inhibitory action in various degree.Meanwhile, Sun Xun measures again tarragon fumigation all has obvious antibacterial action in various degree to oidium schoenleinii, the mutation of oidium schoenleinii Mongolia, Trichophyton concentricum, trichophyton, Trichophyton rubrum, acrothesium floccosum, rust microspore tinea bacterium, sufficient sole of the foot trichophyta, trichophyton interdigitale, dog microspore tinea bacterium, trichophyton gypseum, Shen kirschner born of the same parents in pathogenic dermatophytes such as silk bacterium, Fei Shi yeasts.
Chrysanthemum has obvious bacteria resistance function, chrysanthemum water decoction or water logging agent, and in vitro test is to various pathogens, and influenza virus PR8 and Leptospira all have certain inhibitory action, and MIC is about 1:10-1:80.In Mice Body, bacteriostatic test shows, fresh herb (acrial part) adds water and distills the volatile oil of gained, and to staphylococcus aureus, Escherichia coli, the bacteriostasis such as shigella flexneri are comparatively strong, very weak to Pseudomonas aeruginosa effect, invalid to Diplococcus pneumopniae.
Loguat leaf has obvious bacteria resistance function, and amygdalin 1. can isolate hydrogen cyanide, has cough-relieving, analgesic activity.2. amygdalin hydrolysis produces benzaldehyde the effect suppressing yeast in digestive tract.3. the grease matter of loguat leaf has slight phlegm-dispelling functions.4. zoopery prove loguat leaf decocting liquid have antibacterial, relieving asthma, phlegm-dispelling functions.5. bacteriostatic test: loquat-leaf extract all has obvious inhibitory action to staphylococcus albus, staphylococcus aureus, Diplococcus pneumopniae, shigella flexneri.
Lophatherum gracile has bacteria resistance function, and lophatherum gracile is still containing various active compositions such as terpene, taraxasterol, flavonoids, organic acid, carbohydrates.Lophatherum gracile all has refrigeration function to the heating that a variety of causes causes; There is stronger diuresis, the excretion of urine chloride can be increased simultaneously; Inhibitory action is had to staphylococcus aureus, Streptococcus hemolyticus.
The bark of eucommia has bacteria resistance function, and bark of eucommia chlorogenic acid has stronger antibacterial and anti-inflammation functions, and aucubin and polymer thereof have obvious bacteriostasis, and peach jaurel aglycon has inhibitory action to Gram-negative, positive bacteria.Aucubin has antibacterial, diuresis, and can promote wound healing; Aucubin also can produce obvious antivirus action after preculture together with glucoside.
In sum, garlic, cordate houttuynia, tarragon, chrysanthemum, loguat leaf, lophatherum gracile and folium cortex eucommiae are all good natural bacteriostatic agent, but to bacterium, mould, the inhibitory action of yeast respectively has power, but existing plant bacteriostatic agent is raw material mainly with single plant, although better to a few class commodity harmful bacteria inhibition, but broad-spectrum antibacterial effect is more weak, in addition in extraction process, existing plant bacteriostatic agent many employings ethanol extraction process, and the solvability of ethanol extract in water is lower, because which limit its range of application in commodity industry.
Summary of the invention
An object of the present invention is to provide a kind of water-soluble compound type bacteriostat with bacteriostatic activity.
Two of object of the present invention is to provide the preparation method of above-mentioned aqueous solution compound type bacteriostat.
An object of the present invention is achieved through the following technical solutions: a kind of water-soluble compound type bacteriostat, it is characterized in that: its bacteriostatic active ingredients is by garlic P.E, houttuynia extract, Folium Artemisiae Argyi extract, chrysanthemum extract, loquat-leaf extract, Herba Lophatheri extract and eucommia leaf extract composition.
Garlic P.E in described water-soluble compound type bacteriostat, houttuynia extract, Folium Artemisiae Argyi extract, chrysanthemum extract, loquat-leaf extract, the mass percent of Herba Lophatheri extract and eucommia leaf extract is respectively: garlic P.E 30-40%, houttuynia extract 10-20%, Folium Artemisiae Argyi extract 5-10%, chrysanthemum extract 10-20%, loquat-leaf extract 5-10%, Herba Lophatheri extract 7-10% and eucommia leaf extract 5-10%.
Described garlic P.E, houttuynia extract, Folium Artemisiae Argyi extract, chrysanthemum extract, loquat-leaf extract, Herba Lophatheri extract and eucommia leaf extract are respectively garlic water extract, cordate houttuynia water extract, tarragon water extract, chrysanthemum water extract, loguat leaf water extract, lophatherum gracile water extract and folium cortex eucommiae water extract.
Two of the object of the invention is achieved through the following technical solutions, and a kind of preparation method of above-mentioned water-soluble compound type bacteriostat, is characterized in that: get garlic indescribably with moisture, cordate houttuynia, tarragon, chrysanthemum, loguat leaf, lophatherum gracile and folium cortex eucommiae, obtain garlic water extract, cordate houttuynia water extract, tarragon water extract, chrysanthemum water extract, loguat leaf water extract, lophatherum gracile water extract and folium cortex eucommiae water extract, mix, namely obtain water-soluble compound type bacteriostat.
Described garlic water extract, cordate houttuynia water extract, tarragon water extract, chrysanthemum water extract, loguat leaf water extract, the preparation process of lophatherum gracile water extract and folium cortex eucommiae water extract is:
(1) by garlic, cordate houttuynia, tarragon, chrysanthemum, loguat leaf, lophatherum gracile and folium cortex eucommiae are cut into uniform thin slice after cleaning, and in 40 ~ 50 DEG C of oven dry, obtain garlic dry plate, cordate houttuynia dry plate, tarragon dry plate, chrysanthemum dry plate, loguat leaf dry plate, lophatherum gracile dry plate and folium cortex eucommiae dry plate;
(2) by garlic dry plate, cordate houttuynia dry plate, tarragon dry plate, chrysanthemum dry plate, loguat leaf dry plate, lophatherum gracile dry plate and folium cortex eucommiae dry plate are pulverized, by obtaining garlic dry powder after 40 ~ 60 mesh sieves, cordate houttuynia dry powder, tarragon dry powder, chrysanthemum dry powder, loguat leaf dry powder, lophatherum gracile dry powder and folium cortex eucommiae dry powder;
(3) garlic dry powder is taken, cordate houttuynia dry powder, tarragon dry powder, chrysanthemum dry powder, loguat leaf dry powder, adds distilled water after lophatherum gracile dry powder and folium cortex eucommiae dry powder, wherein garlic dry powder, cordate houttuynia dry powder, tarragon dry powder, chrysanthemum dry powder, loguat leaf dry powder, the weight ratio of lophatherum gracile dry powder and folium cortex eucommiae dry powder and distilled water is 1:20 ~ 30, in 50 ~ 70 DEG C of water-bath refluxing extraction 1 ~ 3 time, each extraction 3 ~ 5 hours, merges extract;
(4) extract carries out vacuum filtration, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/6 ~ 1/10;
(5) concentrated extracting solution is carried out vacuum drying, obtain garlic water extract, cordate houttuynia water extract, tarragon water extract, chrysanthemum water extract, loguat leaf water extract, lophatherum gracile water extract and folium cortex eucommiae water extract.
Compared with prior art, the present invention has the following advantages:
1, with garlic, cordate houttuynia, tarragon, chrysanthemum, loguat leaf, lophatherum gracile and folium cortex eucommiae etc. respectively have strong and weak plant to be raw material to the inhibitory action of bacterium, mould, yeast, effective antimicrobial spectrum expanding bacteriostatic agent, overcoming existing plant bacteriostatic agent mainly with single plant is raw material, the defect that broad-spectrum antibacterial effect is more weak, scope of restraining fungi is wider, better effects if.
2, the present invention adopts water extraction to extract garlic, cordate houttuynia, tarragon, chrysanthemum, loguat leaf, lophatherum gracile and folium cortex eucommiae, the bacteriostatic agent made water-soluble very good, overcomes existing plant bacteriostatic agent and adopts the defect that extract solvability in water of alcohol extraction process gained is lower, has expanded its range of application in commodity industry, namely as a kind of safety, efficiently natural antiseptic agent, all kinds of commodity industry can be widely used in.
3, from garlic dry plate, cordate houttuynia dry plate, tarragon dry plate, chrysanthemum dry plate, loguat leaf dry plate, extracts water extract in lophatherum gracile dry plate and folium cortex eucommiae and prepares bacteriostatic agent, can garlic, cordate houttuynia, tarragon, chrysanthemum, loguat leaf, the harvest season of lophatherum gracile and folium cortex eucommiae purchases in a large number with lower expense, makes dry plate for subsequent use, can not only produce throughout the year, can also product cost be reduced.
Embodiment
Below in conjunction with embodiment and comparative example, the present invention is set forth, and protection scope of the present invention is not only confined to following examples.The those of ordinary skill of described technical field, according to content disclosed by the invention, all can realize object of the present invention.
Embodiment one
The following step is prepared water-soluble compound type bacteriostat:
The preparation of 1 garlic P.E
1.1 using commercially available fresh garlic as raw material, is cut into uniform thin slice, in 45 DEG C of oven dry, obtains garlic dry plate after cleaning peeling with slicer;
The garlic dry plate obtained in step 1.1 is pulverized, by obtaining garlic dry powder after 60 mesh sieves by 1.2;
1.3 take the garlic dry powder obtained in a certain amount of step 1.2, with garlic dry powder: the weight ratio of water=1:25 adds distilled water, in 60 DEG C of water-bath refluxing extraction 3 times, each 3 hours, are merged by the extract of said extracted 3 times;
The extract obtained in step 1.3 is carried out vacuum filtration by 1.4, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/8;
The concentrated extracting solution obtained in step 1.4 is carried out spraying dry by 1.5, obtains garlic water extract.
The preparation of 2 houttuynia extracts
2.1 using commercially available fresh cordate houttuynia as raw material, is cut into uniform thin slice, in 45 DEG C of oven dry, obtains cordate houttuynia dry plate after cleaning peeling with slicer;
The cordate houttuynia dry plate obtained in step 2.1 is pulverized, by obtaining cordate houttuynia dry powder after 60 mesh sieves by 2.2;
2.3 take the cordate houttuynia dry powder obtained in a certain amount of step 2.2, with cordate houttuynia dry powder: the weight ratio of water=1:25 adds distilled water, in 60 DEG C of water-bath refluxing extraction 3 times, each 3 hours, are merged by the extract of said extracted 3 times;
The extract obtained in step 2.3 is carried out vacuum filtration by 2.4, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/8;
The concentrated extracting solution obtained in step 2.4 is carried out spraying dry by 2.5, obtains cordate houttuynia water extract.
The preparation of 3 Folium Artemisiae Argyi extracts
3.1 using commercially available fresh tarragon as raw material, is cut into uniform thin slice, in 45 DEG C of oven dry, obtains tarragon dry plate after cleaning peeling with slicer;
The tarragon dry plate obtained in step 3.1 is pulverized, by obtaining tarragon dry powder after 60 mesh sieves by 3.2;
3.3 take the tarragon dry powder obtained in a certain amount of step 3.2, with tarragon dry powder: the weight ratio of water=1:25 adds distilled water, in 60 DEG C of water-bath refluxing extraction 3 times, each 3 hours, are merged by the extract of said extracted 3 times;
The extract obtained in step 3.3 is carried out vacuum filtration by 3.4, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/8;
The concentrated extracting solution obtained in step 3.4 is carried out spraying dry by 3.5, obtains tarragon water extract.
The preparation of 4 chrysanthemum extracts
4.1 using commercially available fresh chrysanthemum as raw material, is cut into uniform thin slice, in 45 DEG C of oven dry, obtains chrysanthemum dry plate after cleaning peeling with slicer;
The chrysanthemum dry plate obtained in step 4.1 is pulverized, by obtaining chrysanthemum dry powder after 60 mesh sieves by 4.2;
4.3 take the chrysanthemum dry powder obtained in a certain amount of step 4.2, with chrysanthemum dry powder: the weight ratio of water=1:25 adds distilled water, in 60 DEG C of water-bath refluxing extraction 3 times, each 3 hours, are merged by the extract of said extracted 3 times;
The extract obtained in step 4.3 is carried out vacuum filtration by 4.4, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/8;
The concentrated extracting solution obtained in step 4.4 is carried out spraying dry by 4.5, obtains chrysanthemum water extract.
The preparation of 5 loquat-leaf extracts
5.1 using commercially available fresh loguat leaf as raw material, is cut into uniform thin slice, in 45 DEG C of oven dry, obtains loguat leaf dry plate after cleaning peeling with slicer;
The loguat leaf dry plate obtained in step 5.1 is pulverized, by obtaining loguat leaf dry powder after 60 mesh sieves by 5.2;
5.3 take the loguat leaf dry powder obtained in a certain amount of step 5.2, with loguat leaf dry powder: the weight ratio of water=1:25 adds distilled water, in 60 DEG C of water-bath refluxing extraction 3 times, each 3 hours, are merged by the extract of said extracted 3 times;
The extract obtained in step 5.3 is carried out vacuum filtration by 5.4, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/8;
The concentrated extracting solution obtained in step 5.4 is carried out spraying dry by 5.5, obtains loguat leaf water extract.
The preparation of 6 Herba Lophatheri extracts
6.1 using commercially available fresh lophatherum gracile as raw material, is cut into uniform thin slice, in 45 DEG C of oven dry, obtains lophatherum gracile dry plate after cleaning peeling with slicer;
The lophatherum gracile dry plate obtained in step 6.1 is pulverized, by obtaining lophatherum gracile dry powder after 60 mesh sieves by 6.2;
6.3 take the lophatherum gracile dry powder obtained in a certain amount of step 6.2, with lophatherum gracile dry powder: the weight ratio of water=1:25 adds distilled water, in 60 DEG C of water-bath refluxing extraction 3 times, each 3 hours, are merged by the extract of said extracted 3 times;
The extract obtained in step 6.3 is carried out vacuum filtration by 6.4, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/8;
The concentrated extracting solution obtained in step 6.4 is carried out spraying dry by 6.5, obtains lophatherum gracile water extract.
The preparation of 7 eucommia leaf extracts
7.1 using commercially available fresh folium cortex eucommiae as raw material, is cut into uniform thin slice, in 45 DEG C of oven dry, obtains folium cortex eucommiae dry plate after cleaning peeling with slicer;
The folium cortex eucommiae obtained in step 7.1 is pulverized, by obtaining folium cortex eucommiae dry powder after 60 mesh sieves by 7.2;
7.3 take the folium cortex eucommiae dry powder obtained in a certain amount of step 7.2, with folium cortex eucommiae dry powder: the weight ratio of water=1:25 adds distilled water, in 60 DEG C of water-bath refluxing extraction 3 times, each 3 hours, are merged by the extract of said extracted 3 times;
The extract obtained in step 7.3 is carried out vacuum filtration by 7.4, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrated extracting solution when filtrate volume narrows down to original 1/8;
The concentrated extracting solution obtained in step 7.4 is carried out spraying dry by 7.5, obtains folium cortex eucommiae water extract.
4. the preparation of bacteriostatic agent
Above-mentioned garlic P.E 34% is taken, houttuynia extract 17%, Folium Artemisiae Argyi extract 7%, chrysanthemum extract 17%, loquat-leaf extract 8%, Herba Lophatheri extract 9% and eucommia leaf extract 8% by the percentage by weight of each component.Carry out composite, after mixing, obtain water-soluble compound type bacteriostat sample.
Embodiment two
The preparation of bacteriostatic agent
Above-mentioned garlic P.E 35% is taken, houttuynia extract 15%, Folium Artemisiae Argyi extract 8%, chrysanthemum extract 16%, loquat-leaf extract 8%, Herba Lophatheri extract 9% and eucommia leaf extract 9% by the percentage by weight of each component.Carry out composite, after mixing, obtain water-soluble compound type bacteriostat sample.
Embodiment three
The preparation of bacteriostatic agent
Above-mentioned garlic P.E 36% is taken, houttuynia extract 16%, Folium Artemisiae Argyi extract 8%, chrysanthemum extract 16%, loquat-leaf extract 8%, Herba Lophatheri extract 9% and eucommia leaf extract 7% by the percentage by weight of each component.Carry out composite, after mixing, obtain water-soluble compound type bacteriostat sample.
Experimental example:
Get the water-soluble compound type bacteriostat that above-described embodiment one to three obtains, chrysanthemum water extract, cordate houttuynia water extract, the sample of garlic water extract carries out antibacterial contrast experiment, and the bacteriostatic activity evaluation drawn is in table 1
Bacterial classification
(1), bacterium: staphylococcus aureus, salmonella, hay bacillus, Escherichia coli
(2), fungi: aspergillus niger, aspergillus oryzae, Penicillium citrinum, brewer's yeast, rhodotorula glutinis, Candida
Medium
(1) inoculum: LB
As solid LB media will be configured, add 15.0g agarose/L
(2) fungus culture medium: potato culture (PDA)
Equipment and galenical
Culture dish (90mm), filter paper, triangle rake, oese, test tube
Galenical: garlic P.E, houttuynia extract, chrysanthemum extract, Folium Artemisiae Argyi extract, loquat-leaf extract, Herba Lophatheri extract, eucommia leaf extract, compound botanical extract
Experimental procedure
Minimal inhibitory concentration measures (MIC)-test tube method
Test tube method test is done to each tested bacterial classification.
Medium is sub-packed in test tube (18mm × 180mm) with every pipe final volume 5m1, at 120 DEG C, sterilizing 20min under 0.1MPa condition, when sterilising medium is cooled to about 50 DEG C, sterile working adds sample and fully mixes, doubling dilution becomes (volume ratio) series concentration, sterile water compares, respectively bevel.Get well-grown tested bacterium streak inoculation on inclined-plane.Bacterium is put in 30 DEG C of incubators, and observe bacteria growing state in 24h, fungi is put in 27 DEG C of incubators, and observed result after 72h, arranges repeating groups.Range estimation with following standard recording: bacterium is completely without growth " "; Bacteria growing is slow, the bacterium colony very little "+" of formation: bacteria growing forms bacterium colony comparatively large " ++ " than very fast; Bacteria growing rapidly but bacterium colony than normally little " +++ "; Bacteria growing is rapid and normal phase is same " +++ ten ".Taking the complete not minimum sample concentration of growth tube is minimum inhibitory concentration (MIC).Table 1: chrysanthemum extract, houttuynia extract, the minimum inhibitory concentration of garlic P.E and water-soluble compound type bacteriostat
From table 1, water extract is to the inhibitory action had for examination bacterium in various degree.Wherein, chrysanthemum water extract is to staphylococcus aureus, salmonella, hay bacillus, the inhibitory action of the bacteriums such as Escherichia coli is the strongest, secondly be garlic water extract, be cordate houttuynia water extract again, cordate houttuynia water extract is to aspergillus oryzae, and the inhibitory action of the mould such as aspergillus niger and Penicillium citrinum is the strongest, next is garlic P.E, be chrysanthemum water extract again, cordate houttuynia water extract is to brewer's yeast, and the inhibitory action of the yeast such as rhodotorula glutinis and Candida is the strongest, next is chrysanthemum water extract, is garlic water extract again.
As can also be seen from Table 1, with the bacteriostatic agent of the composite preparation of above-mentioned water extract, to staphylococcus aureus, the inhibitory action of aspergillus niger and aspergillus oryzae and the inhibitory action of other microorganism are all equal to or are better than the strongest single plant extracts of inhibitory action, illustrate that the fungistatic effect through the composite plant bacteriostatic agent obtained is better, antimicrobial spectrum is larger, has wide spectrum, efficient bacteriostasis.
Claims (10)
1. a water-soluble compound type bacteriostat, is characterized in that: it is mainly by garlic P.E, houttuynia extract, Folium Artemisiae Argyi extract, chrysanthemum extract, loquat-leaf extract, Herba Lophatheri extract and eucommia leaf extract composition.
2. water-soluble compound type bacteriostat according to claim 1, it is characterized in that: described garlic P.E, houttuynia extract, Folium Artemisiae Argyi extract, chrysanthemum extract, loquat-leaf extract, the mass percent of Herba Lophatheri extract and eucommia leaf extract is respectively: garlic P.E 30-40%, houttuynia extract 10-20%, Folium Artemisiae Argyi extract 5-10%, chrysanthemum extract 10-20%, loquat-leaf extract 5-10%, Herba Lophatheri extract 7-10% and eucommia leaf extract 5-10%.
3. water-soluble compound type bacteriostat according to claim 1 and 2, its characteristic is: described garlic P.E, houttuynia extract, Folium Artemisiae Argyi extract, chrysanthemum extract, loquat-leaf extract, Herba Lophatheri extract and eucommia leaf extract are respectively garlic water extract, cordate houttuynia water extract, tarragon water extract, chrysanthemum water extract, loguat leaf water extract, lophatherum gracile water extract and folium cortex eucommiae water extract.
4. prepare a method for the water-soluble compound type bacteriostat described in claim 1 or 2, it is characterized in that: get garlic indescribably with moisture, cordate houttuynia, tarragon, chrysanthemum, loguat leaf, lophatherum gracile and folium cortex eucommiae, obtain garlic water extract, cordate houttuynia water extract, tarragon water extract, chrysanthemum water extract, loguat leaf water extract, lophatherum gracile water extract and folium cortex eucommiae water extract, mix, namely obtain water-soluble compound type bacteriostat.
5. the preparation method of water-soluble compound type bacteriostat according to claim 4: it is characterized in that: the preparation process of described garlic P.E:
A, is cut into uniform thin slice after being cleaned by garlic, dries, obtain garlic dry plate in 40-50 degree;
Garlic dry plate is pulverized, by obtaining garlic dry powder after 40-60 mesh sieve by B;
C adds distilled water after taking garlic dry powder, and wherein the weight ratio of garlic dry powder and distilled water is 1:20-1:30, in 50-70 degree water-bath refluxing extraction 1-3 time, extracts 3-5 hour at every turn, is merged by extract.
D extract carries out vacuum filtration, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrate extract when narrowing down to original 1/6-1/10 to filtrate volume;
Concentrated extracting solution is carried out vacuum drying by E, obtains garlic water extract.
6. the preparation method of water-soluble compound type bacteriostat according to claim 4: it is characterized in that: the preparation process of described houttuynia extract:
A, is cut into uniform thin slice after being cleaned by cordate houttuynia, dries, obtain cordate houttuynia dry plate in 40-50 degree;
Cordate houttuynia dry plate is pulverized, by obtaining cordate houttuynia dry powder after 40-60 mesh sieve by B;
C adds distilled water after taking cordate houttuynia dry powder, and wherein the weight ratio of cordate houttuynia dry powder and distilled water is 1:20-1:30, in 50-70 degree water-bath refluxing extraction 1-3 time, extracts 3-5 hour at every turn, is merged by extract;
D extract carries out vacuum filtration, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrate extract when narrowing down to original 1/6-1/10 to filtrate volume;
Concentrated extracting solution is carried out vacuum drying by E, obtains cordate houttuynia water extract.
7. the preparation method of water-soluble compound type bacteriostat according to claim 4: it is characterized in that: the preparation process of described Folium Artemisiae Argyi extract:
A, is cut into uniform thin slice after being cleaned by tarragon, dries, obtain tarragon dry plate in 40-50 degree;
Tarragon dry plate is pulverized, by obtaining tarragon dry powder after 40-60 mesh sieve by B;
C adds distilled water after taking tarragon dry powder, and wherein the weight ratio of tarragon dry powder and distilled water is 1:20-1:30, in 50-70 degree water-bath refluxing extraction 1-3 time, extracts 3-5 hour at every turn, is merged by extract;
D extract carries out vacuum filtration, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrate extract when narrowing down to original 1/6-1/10 to filtrate volume;
Concentrated extracting solution is carried out vacuum drying by E, obtains tarragon water extract.
8. the preparation method of water-soluble compound type bacteriostat according to claim 4: it is characterized in that: the preparation process of described chrysanthemum extract:
A, is cut into uniform thin slice after being cleaned by chrysanthemum, dries, obtain chrysanthemum dry plate in 40-50 degree;
Chrysanthemum dry plate is pulverized, by obtaining chrysanthemum dry powder after 40-60 mesh sieve by B;
C adds distilled water after taking chrysanthemum dry powder, and wherein the weight ratio of chrysanthemum dry powder and distilled water is 1:20-1:30, in 50-70 degree water-bath refluxing extraction 1-3 time, extracts 3-5 hour at every turn, is merged by extract;
D extract carries out vacuum filtration, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrate extract when narrowing down to original 1/6-1/10 to filtrate volume;
Concentrated extracting solution is carried out vacuum drying by E, obtains chrysanthemum water extract.
9. the preparation method of water-soluble compound type bacteriostat according to claim 4: it is characterized in that: the preparation process of described loquat-leaf extract:
A is cut into uniform thin slice after being cleaned by loguat leaf, dries, obtain loguat leaf dry plate in 40-50 degree;
Loguat leaf dry plate is pulverized, by obtaining loguat leaf dry powder after 40-60 mesh sieve by B;
C adds distilled water after taking loguat leaf dry powder, and wherein the weight ratio of loguat leaf dry powder and distilled water is 1:20-1:30, in 50-70 degree water-bath refluxing extraction 1-3 time, extracts 3-5 hour at every turn, is merged by extract;
D extract carries out vacuum filtration, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrate extract when narrowing down to original 1/6-1/10 to filtrate volume;
Concentrated extracting solution is carried out vacuum drying by E, obtains loguat leaf water extract.
10. the preparation method of water-soluble compound type bacteriostat according to claim 4: it is characterized in that: described lophatherum gracile or
The preparation process of folium cortex eucommiae leaf extract:
A is cut into uniform thin slice after lophatherum gracile or folium cortex eucommiae being cleaned, and dries, obtain lophatherum gracile or folium cortex eucommiae in 40-50 degree;
Lophatherum gracile or folium cortex eucommiae are pulverized, by obtaining lophatherum gracile or folium cortex eucommiae dry powder after 40-60 mesh sieve by B;
C adds distilled water after taking lophatherum gracile or folium cortex eucommiae dry powder, and wherein the weight ratio of lophatherum gracile or folium cortex eucommiae dry powder and distilled water is 1:20-1:30, in 50-70 degree water-bath refluxing extraction 1-3 time, extracts 3-5 hour at every turn, is merged by extract;
D extract carries out vacuum filtration, and filtrate is concentrated on Rotary Evaporators, stops concentrated, obtain concentrate extract when narrowing down to original 1/6-1/10 to filtrate volume;
Concentrated extracting solution is carried out vacuum drying by E, obtains lophatherum gracile or folium cortex eucommiae water extract.
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| CN108419828A (en) * | 2018-03-21 | 2018-08-21 | 江西农业大学 | A kind of citrus comprising loquat-leaf extract adopts rear plant antistaling agent |
| CN109258788A (en) * | 2018-08-09 | 2019-01-25 | 兴义市天瑞核桃种植农民专业合作社 | A kind of preservation method of green rind walnut |
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| CN110959773A (en) * | 2019-12-02 | 2020-04-07 | 广西壮族自治区水产科学研究院 | Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed |
| CN110959773B (en) * | 2019-12-02 | 2023-03-24 | 广西壮族自治区水产科学研究院 | Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed |
| CN113598202A (en) * | 2021-08-11 | 2021-11-05 | 广东开放大学（广东理工职业学院） | Antibacterial composition and application thereof in preparation of biodegradable plastic |
| CN113598202B (en) * | 2021-08-11 | 2022-06-24 | 广东开放大学（广东理工职业学院） | Antibacterial composition and application thereof in preparation of biodegradable plastic |
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