CN110959773B - Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed - Google Patents
Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed Download PDFInfo
- Publication number
- CN110959773B CN110959773B CN201911213405.2A CN201911213405A CN110959773B CN 110959773 B CN110959773 B CN 110959773B CN 201911213405 A CN201911213405 A CN 201911213405A CN 110959773 B CN110959773 B CN 110959773B
- Authority
- CN
- China
- Prior art keywords
- feed
- parts
- portions
- tilapia
- preventing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
- A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Mycology (AREA)
- Marine Sciences & Fisheries (AREA)
- Biomedical Technology (AREA)
- Inorganic Chemistry (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention belongs to the technical field of feeds, and particularly relates to a feed additive and a feed for preventing streptococcicosis of tilapia, and a preparation method and application thereof. The invention discloses a feed additive for preventing streptococcicosis of tilapia mossambica, which comprises zymosan, mint leaves, agastache rugosus, mulberry leaves, houttuynia cordata, lophatherum gracile, chinese violet, liquorice and poria cocos. The feed additive provided by the invention does not contain antibiotic components, and the results of the examples show that the feed prepared by using the feed additive provided by the invention can improve the prevention capability of the tilapia on streptococcal diseases, can accelerate the growth of the tilapia, improve the immunity and disease resistance of the tilapia, and can replace the feed containing antibiotic drugs for preventing the tilapia streptococcal diseases, so that the use of the antibiotic drugs in the feed for preventing the tilapia streptococcal diseases is reduced.
Description
Technical Field
The invention belongs to the technical field of fish feed, and particularly relates to feed additive and feed for preventing streptococcicosis of tilapia, and a preparation method and application thereof.
Background
Tilapia mossambica is one of the dominant freshwater aquaculture varieties in China, and the aquaculture area, the yield and the processed products are the top in the world. However, with the continuous expansion of aquaculture area and the continuous increase of aquaculture density in recent years, the aquaculture environment is worsened, the tilapia suffers from streptococcicosis in large area, the mortality rate is increased, and the economic loss is large. Tilapia mossambica streptococcicosis is a fulminant bacterial disease caused by streptococcus agalactiae and streptococcus lactiae, the death rate is up to 30-90%, no absolute effective treatment scheme exists at present, and antibiotics are mainly used for treatment; with the large amount and frequent use of antibiotics, the problems of environmental pollution, drug residue, food safety and the like are caused, thereby affecting the export of tilapia products in China. In addition, streptococcus damages tissues such as brain, liver and kidney of tilapia, functional disorder occurs, and the tilapia is difficult to cure, and the tilapia continues to die continuously once the medicine is stopped.
Currently, the severity of streptococcal disease in tilapia is prevention. Therefore, the reduction of the use of antibiotic drugs in the feed for preventing tilapia streptococcus becomes the research direction of the people.
Disclosure of Invention
In view of the above, the invention provides a feed additive for preventing tilapia streptococcicosis. The feed additive provided by the invention does not contain antibiotic components, not only can improve the prevention capability of the tilapia on streptococcicosis, but also can accelerate the growth of the tilapia, improve the immunity and disease resistance of the tilapia, and can replace a feed containing antibiotic drugs for preventing the tilapia streptococcicosis, so that the use of the antibiotic drugs in the feed for preventing the tilapia streptococcicosis is reduced.
In order to achieve the above object, the present invention provides the following technical solutions;
the invention provides a feed additive for preventing streptococcicosis of tilapia, which comprises the following components: zymosan, mint leaves, agastache rugosus, mulberry leaves, houttuynia cordata, lophatherum gracile, chinese violet, liquorice and poria cocos.
Preferably, the feed additive consists of the following components in parts by mass: 5 to 20 portions of zymosan, 10 to 20 portions of mint leaves, 10 portions of wrinkled gianthyssop herb, 10 to 20 portions of mulberry leaves, 10 to 20 portions of heartleaf houttuynia herb, 5 to 10 portions of common lophatherum herb, 10 to 20 portions of Chinese violet, 5 portions of liquorice and 5 portions of tuckahoe.
The invention provides a feed for preventing streptococcicosis of tilapia, which is prepared from the following raw materials in parts by mass: 84-116 parts of basal feed and 2-6 parts of feed additive in the technical scheme.
Preferably, the basic feed comprises the following components in parts by mass: 30 to 35 portions of protein, 4 to 8 portions of fat, 0.6 to 1.0 portion of calcium, 0.8 to 1.2 portions of phosphorus, 1.5 to 2.0 portions of lysine and 0.3 to 0.6 portion of methionine.
Preferably, the basic feed is prepared from the following raw materials in parts by mass: 3 to 6 portions of fish meal, 4 to 8 portions of chicken meal, 35 to 40 portions of bean pulp, 15 to 20 portions of peanut bran, 10 to 17 portions of corn bran, 10 to 15 portions of cassava starch, 3 to 5 portions of soybean oil, 0.5 portion of salt, 0.5 portion of choline chloride, and Ca (H) 2 PO 4 ) 2 1-2 parts and premix 2233#2 parts of omnivorous fish.
The invention also provides a preparation method of the feed in the technical scheme, which comprises the following steps:
(1) Pulverizing fish powder, chicken powder, soybean meal, peanut bran, corn bran, cassava starch, salt, and premix 2233# for omnivorous fish, adding choline chloride and Ca (H) 2 PO 4 ) 2 Mixing with feed additive, and stirring to obtain pre-mixed feed;
(2) And spraying soybean oil on the pre-mixed feed, and cooling to obtain the feed for preventing the streptococcal disease of the tilapia.
Preferably, the step (1) of mixing fish meal, chicken meal, soybean meal, peanut bran, corn bran, cassava starch, salt and the omnivorous fish premix 2233# and then sieving;
preferably, before the soybean oil spraying in the step (2), the pre-mixed feed is sequentially subjected to crushing, impurity removal, secondary mixing, puffing material preparation and drying treatment.
Preferably, the pulverization is ultramicro pulverization, and the aperture sizes of the sieves are all 80 meshes.
Preferably, the mass ratio of the sprayed soybean oil in the step (2) to the pre-mixed feed is 25-40: 75 to 60 percent.
The invention also provides a use method of the feed, which comprises the following steps: feeding for 3-7 days every 15 days in 4-6 months and 10-12 months, wherein the daily feeding amount is 2-3% of the weight of the fish; feeding for 3-5 days every 7-10 days for 7-9 months, wherein the daily feeding amount is 2-3% of the weight of the fish or feeding every day.
The invention provides a feed additive for preventing streptococcicosis of tilapia, which comprises the following components: zymosan, mint leaves, agastache rugosus, mulberry leaves, houttuynia cordata, lophatherum gracile, chinese violet, liquorice and poria cocos. The feed additive for preventing streptococcicosis of tilapia is not added with any antibiotic medicine, and a proper amount of feed additive is added on the basis of balanced nutrition of each basic ingredient, so that the immunity of tilapia is improved, and the occurrence of streptococcicosis is prevented. The embodiment proves that the feed prepared from the feed additive for preventing the streptococcal disease of tilapia can improve the prevention capability of the tilapia on the streptococcal disease, can accelerate the growth of the tilapia, improve the immunity and disease resistance of the tilapia, and can replace a feed containing antibiotic drugs for preventing the streptococcal disease of the tilapia, so that the use of the antibiotic drugs in the feed for preventing the streptococcal disease of the tilapia is reduced.
Detailed Description
The invention provides a feed additive for preventing streptococcicosis of tilapia, which comprises the following components: zymosan, mint leaves, agastache rugosus, mulberry leaves, houttuynia cordata, lophatherum gracile, chinese violet, liquorice and poria cocos; the composition preferably comprises the following components in parts by mass: 5 to 20 portions of zymosan, 10 to 20 portions of mint leaves, 10 portions of wrinkled gianthyssop herb, 10 to 20 portions of mulberry leaves, 10 to 20 portions of heartleaf houttuynia herb, 5 to 10 portions of common lophatherum herb, 10 to 20 portions of Chinese violet, 5 portions of liquorice and 5 portions of tuckahoe.
The feed additive of the invention preferably comprises 5-20 parts of zymosan by mass, and more preferably comprises 10-15 parts of zymosan by mass.
The feed additive of the invention preferably comprises 10-20 parts of mint leaves, and further preferably comprises 14-16 parts of mint leaves by mass based on the parts by mass of the zymosan.
The feed additive of the invention preferably comprises 10 parts of agastache rugosus based on the mass parts of zymosan.
The feed additive provided by the invention preferably comprises 10-20 parts of mulberry leaves, and more preferably 14-16 parts of zymosan in parts by mass.
The feed additive of the invention preferably comprises 10 to 20 parts of houttuynia cordata, and more preferably comprises 14 to 16 parts of zymosan by mass.
The feed additive provided by the invention preferably comprises 5-10 parts of lophatherum gracile by mass, and more preferably comprises 7-8 parts of zymosan by mass.
The feed additive provided by the invention preferably comprises 10-20 parts of Chinese violet, and more preferably comprises 15 parts of zymosan by mass. The feed additive preferably comprises 5 parts of liquorice by mass based on the mass parts of the zymosan; 5 parts of poria cocos.
In the present invention, the particle size of the mint leaves, the agastache rugosus, the mulberry leaves, the houttuynia cordata, the lophatherum gracile, the Chinese violet, the licorice and the poria cocos is preferably 0.180mm. The feed additive has reasonable compatibility of components, and has the effects of clearing away heat and toxic materials, removing liver heat and invigorating spleen, reducing fat and activating blood, inhibiting bacteria and diminishing inflammation, improving immunity and improving anti-stress capability; the folium Menthae has stomach invigorating, antiinflammatory and repercussive effects; herba Agastaches has effects of killing bacteria, relieving summer heat and invigorating stomach; the mulberry leaves have the effects of diminishing inflammation, removing dryness, cooling blood and resisting stress; the herba Houttuyniae has antibacterial, antiviral and immunity enhancing effects; lophatherum gracile has the effects of reducing fever, relieving restlessness, diminishing inflammation and detoxifying; the herba Violae has effects of clearing away heat and toxic materials, cooling blood, and relieving swelling; the liquorice strengthens the spleen and stomach and blends the medicines; poria has effects of clearing heat and invigorating spleen.
The preparation method of the feed additive for preventing the streptococcicosis of the tilapia does not have special requirements, and the components are mixed by adopting a composition mixing method well known by the technical personnel in the field.
The invention provides a feed for preventing streptococcicosis of tilapia, which is prepared from the following raw materials in parts by mass: 84-116 parts of basal feed and 2-6 parts of feed additive.
The feed for preventing the streptococcicosis of the tilapia comprises 84 to 116 parts of basic feed, preferably 90 to 110 parts, and more preferably 100 parts.
From the composition, the basic feed disclosed by the invention preferably comprises the following components in parts by mass: 30 to 35 portions of protein, 4 to 8 portions of fat, 0.6 to 1.0 portion of calcium, 0.8 to 1.2 portions of phosphorus, 1.5 to 2.0 portions of lysine and 0.3 to 0.6 portion of methionine.
The basic feed provided by the invention comprises 30-35 parts of protein, preferably 32-33 parts.
The basic feed provided by the invention comprises 0.6-1.0 part of calcium, preferably 0.8-0.9 part of calcium by mass part of the protein.
The basic feed provided by the invention comprises 0.8-1.2 parts of phosphorus, preferably 0.9-1.1 parts of phosphorus by mass.
The basic feed provided by the invention comprises 1.5-2.0 parts of lysine by mass of the protein, preferably 1.7-1.9 parts.
The basic feed provided by the invention comprises 0.3-0.6 part of methionine, preferably 0.4-0.5 part of methionine based on the mass part of the protein.
The basic feed provided by the invention comprises 4-8 parts of fat, preferably 5-7 parts of fat based on the mass parts of the protein.
The basic feed provided by the invention is prepared from the raw materials of, by mass, 3-6 parts of fish meal, and further 4-5 parts of fish meal.
On the basis of the mass parts of the fish meal, the preparation raw materials of the basic feed preferably comprise 4-8 parts of chicken meal, and further preferably comprise 5-7 parts of chicken meal; 35-40 parts of soybean meal, and further preferably 37-39 parts of soybean meal; 15-20 parts of peanut bran, and further preferably 17-19 parts of peanut bran; 10-17 parts of corn bran, and further preferably 14-16 parts of corn bran; 10-15 parts of cassava starch, and further preferably 12-13 parts of cassava starch; 3-5 parts of soybean oil, and further preferably 4 parts; ca (H) 2 PO 4 ) 2 1 to 2 parts, and more preferably 2 parts. The parts per mass of the components of the feed additive defined hereinbefore differs from the parts per mass of the components of the basal feed defined hereinbefore in the determination of the relative amounts of the components of the feed additive.
Based on the mass parts of the fish meal in the basal feed, the preparation raw materials of the feed for preventing the streptococcal disease of the tilapia preferably comprise 2 to 6 parts of feed additives (namely the mass ratio of the basal feed to the feed additives is 84 to 116:2 to 6), and further preferably comprise 3 to 5 parts of feed additives. According to the invention, the feed additive is added into the basic feed, so that the feed for preventing streptococcicosis of tilapia has the effects of clearing away heat and toxic materials, clearing liver and tonifying spleen, reducing fat and activating blood, inhibiting bacteria and diminishing inflammation, and can improve the immunity and anti-stress capability of tilapia.
The invention also provides a preparation method of the feed in the technical scheme, which comprises the following steps:
(1) Mixing fish powder, chicken powder, soybean meal, peanut bran, corn bran, cassava starch, salt, omnivorous fish premix 2233#, choline chloride, and Ca (H) 2 PO 4 ) 2 Mixing with feed additive to obtain pre-mixed feed;
(2) And spraying soybean oil on the pre-mixed feed to obtain the feed for preventing the streptococcal disease of the tilapia.
The invention pulverizes fish meal, chicken powder, bean pulp, peanut bran, corn bran, cassava starch, salt, omnivorous fish premix 2233#, and mixes with choline chloride and Ca (H) 2 PO 4 ) 2 Mixing with the above feed additive to obtain pre-mixed feed. Preferably, the pulverization is ultramicro pulverization; the method comprises the following steps of mixing fish meal, chicken meal, bean pulp, peanut bran, corn bran, cassava starch, salt and a pre-mixed omnivorous fish 2233#, and preferably further sieving, wherein the size of a sieve pore is 80 meshes; the premix 2233# of the omnivorous fish is purchased from Guangzhou Coffy-spread biotechnology limited company, and the invention is used for fish meal, chicken meal, bean pulp, peanut bran, corn bran, cassava starch, salt, choline chloride and Ca (H) 2 PO 4 ) 2 The source of (A) is not particularly limited, and conventional commercially available products in the art may be used.
After the pre-mixed feed is obtained, the invention sprays soybean oil on the pre-mixed feed to obtain the feed for preventing the streptococcal disease of the tilapia. In the invention, the mass ratio of the sprayed soybean oil to the pre-mixed feed is more preferably 3-4.5: 100, more preferably 3.5 to 4:100, most preferably 3.5:100. before the soybean oil spraying, the pre-mixed feed is preferably subjected to crushing, impurity removal, secondary mixing, puffing material preparation and drying treatment in sequence. Preferably, the pulverization is ultrafine pulverization. The invention has no special requirements for the specific implementation modes of oil injection mode, crushing, impurity removal, puffing material preparation and drying treatment, and can adopt the methods well known by the technical personnel in the field.
The invention also provides a using method of the feed in the technical scheme, which comprises the following steps: feeding for 3-7 days every 15 days in 4-6 months and 10-12 months; feeding for 3-5 days every 7-10 days in 7-9 months, more preferably feeding every day, wherein the daily feeding amount is preferably 2-5%, more preferably 3-4% of the weight of the fish. The feeding mode is not particularly limited, and the feeding mode can be realized by utilizing the conventional feeding mode in the field.
The following examples are provided to illustrate the feed additive and feed for preventing streptococcal disease of tilapia mossambica, and the preparation method and application thereof in detail, but they should not be construed as limiting the scope of the present invention.
Example 1
(1) Preparing a feed additive: weighing the following raw materials in parts by weight: 20 parts of zymosan, 8 parts of herba polygoni avicularis, 10 parts of wrinkled gianthyssop, 20 parts of mulberry leaf, 10 parts of heartleaf houttuynia herb, 10 parts of common lophatherum herb, 10 parts of Chinese violet herb, 5 parts of liquorice and 5 parts of tuckahoe, wherein the herba polygoni avicularis, the herba pogostemi, the mulberry leaf, the heartleaf houttuynia herb, the common lophatherum herb, the Chinese violet herb, the liquorice and the tuckahoe are subjected to superfine grinding, sieved by a 80-mesh sieve and then uniformly mixed with the zymosan to obtain the feed additive.
(2) Weighing the following raw materials in parts by weight: 4 parts of fish meal, 6 parts of chicken meal, 36.5 parts of bean pulp, 16.5 parts of peanut bran, 12.5 parts of corn bran, 12 parts of cassava starch, 3.5 parts of soybean oil, 0.5 part of salt, 0.5 part of choline chloride and Ca (H) 2 PO 4 ) 2 2 parts of omnivorous fish premix 2233#2 parts of feed additive 3 parts. Micronizing fish powder, chicken powder, bean cake, peanut bran, corn bran, tapioca starch, and salt, sieving with 80 mesh sieve, adding choline chloride and Ca (H) 2 PO 4 ) 2 And the omnivorous fish premix 2233# and the feed additive are mixed and stirred evenly to obtain the pre-mixed feed.
(3) Carrying out superfine grinding, impurity removal, secondary mixing, puffing and granulating, drying, spraying soybean oil, cooling and packaging on the prepared feed to obtain a finished product.
Example 2
(1) Preparing a feed additive: weighing the following raw materials in parts by weight: 8 parts of zymosan, 10 parts of herba polygoni avicularis, 10 parts of agastache rugosus, 18 parts of mulberry leaves, 17 parts of houttuynia cordata, 10 parts of lophatherum gracile, 17 parts of Chinese violet, 5 parts of liquorice and 5 parts of poria cocos, crushing, sieving by a 80-mesh sieve, and uniformly mixing with the zymosan to obtain the feed additive.
(2) Weighing the following raw materials in parts by weight: 4 parts of fish meal, 6 parts of chicken meal, 36.5 parts of bean pulp, 16.5 parts of peanut bran, 10.5 parts of corn bran, 12 parts of cassava starch, 3.5 parts of soybean oil, 0.5 part of salt, 0.5 part of choline chloride and Ca (H) 2 PO 4 ) 2 2 parts of omnivorous fish premix 2233#2 parts of feed additive 5 parts. Micronizing fish powder, chicken powder, bean cake, peanut bran, corn bran, tapioca starch, and salt, sieving with 80 mesh sieve, adding choline chloride and Ca (H) 2 PO 4 ) 2 And the omnivorous fish premix 2233# and the feed additive are mixed and stirred evenly to obtain the pre-mixed feed.
(3) Carrying out superfine grinding, impurity removal, secondary mixing, puffing and granulating, drying, spraying soybean oil, cooling and packaging on the prepared feed to obtain a finished product.
Example 3
(1) Preparing a feed additive: weighing the following raw materials in parts by weight: 10 parts of zymosan, 10 parts of herba polygoni avicularis, 10 parts of agastache rugosus, 15 parts of mulberry leaf, 15 parts of houttuynia cordata, 10 parts of lophatherum gracile, 20 parts of herba violae, 5 parts of liquorice and 5 parts of poria cocos, wherein the herba polygoni avicularis, the agastache rugosus, the mulberry leaf, the houttuynia cordata, the herba lophatherum gracile, the herba violae, the liquorice and the poria cocos are subjected to superfine grinding, sieved by a 80-mesh sieve and then uniformly mixed with the zymosan to obtain the feed additive.
(2) Weighing the following raw materials in parts by weight: 6 parts of fish meal, 4 parts of chicken meal, 35 parts of bean pulp, 16.5 parts of peanut bran, 11 parts of corn bran, 14 parts of cassava starch, 3.5 parts of soybean oil, 0.5 part of salt, 0.5 part of choline chloride and Ca (H) 2 PO 4 ) 2 2 parts of omnivorous fish premix 2233#, 2 parts of feed additive and 5 parts of feed additive. Micronizing fish powder, chicken powder, bean cake, peanut bran, corn bran, cassava starch, and salt, and sieving with 8 mesh sieveSieving with 0 mesh sieve, adding choline chloride and Ca (H) 2 PO 4 ) 2 And the omnivorous fish premix 2233# and the feed additive are mixed and stirred evenly to obtain the pre-mixed feed.
(3) Carrying out superfine grinding, impurity removal, secondary mixing, puffing and granulating, drying, spraying soybean oil, cooling and packaging on the prepared feed to obtain a finished product.
Example 4
(1) Preparing a feed additive: weighing the following raw materials in parts by weight: 10 parts of zymosan, 8 parts of herba polygoni avicularis, 12 parts of agastache rugosus, 17 parts of folium mori, 15 parts of houttuynia cordata, 10 parts of lophatherum gracile, 18 parts of herba violae, 5 parts of liquorice and 5 parts of poria cocos, wherein the herba polygoni avicularis, the agastache rugosus, the folium mori, the houttuynia cordata, the herba lophatherum gracile, the herba violae, the liquorice and the poria cocos are subjected to superfine grinding, sieved by a 80-mesh sieve and then uniformly mixed with the zymosan to obtain the feed additive.
(2) Weighing the following raw materials in parts by weight: 5 parts of fish meal, 5 parts of chicken meal, 36.5 parts of bean pulp, 15.5 parts of peanut bran, 12 parts of corn bran, 14 parts of cassava starch, 3.5 parts of soybean oil, 0.5 part of salt, 0.5 part of choline chloride and Ca (H) 2 P O 4 ) 2 2 parts of omnivorous fish premix 2233#2 parts of feed additive 4 parts. Micronizing fish powder, chicken powder, bean cake, peanut bran, corn bran, tapioca starch, and salt, sieving with 80 mesh sieve, adding choline chloride and Ca (H) 2 PO 4 ) 2 And the omnivorous fish premix 2233# and the feed additive are mixed and stirred evenly to obtain the pre-mixed feed.
(3) Carrying out superfine grinding, impurity removal, secondary mixing, puffing and granulating, drying, spraying soybean oil, cooling and packaging on the pre-mixed feed to obtain a finished product.
Application example 1
A test method for feeding tilapia by using compound feed for preventing streptococcicosis of tilapia comprises the following steps:
the test fish is the tilapia from self-bred Aoni, and the specification is 200 +/-15 g; the test fish were 270 in total, and were divided into 9 groups of 30 fish.
Processing one: feeding special feed for the Tilapia mossambica in the 1 st to 3 rd groups;
and (5) processing: feeding special feed for the tilapia in 4 th to 6 th groups;
and (3) treatment III: feeding the feed obtained in the example 1 to the 7 th to 9 th groups;
the test is carried out by hanging a 2m multiplied by 1m net cage in a 6m multiplied by 8m multiplied by 1m cement pool at the temperature of 26-32 ℃; 2 inflation heads/box are arranged in the pool; feeding the test fish for 2 times/day, wherein the feeding amount is 3% -5% of the weight of the fish, the feeding time is respectively from 8 a.m. to 17 a.m.:00, the test is from the beginning of 7 months to the end of 8 months for 8 weeks, the fish is stopped for 1d before detection, the weight of the fish is measured, then a challenge test is carried out, a pectoral fin injection mode is adopted, a challenge bacteria solution is a streptococcus agalactiae strain separated and identified in a laboratory, the streptococcus agalactiae strain is cultured after activation, and the physiological saline is used for diluting the bacteria solution to the concentration of 1 × 10 8 CFU/ml, the injection amount is 0.2ml, the infection and death condition of the fish are observed after injection, the fish are fished out in time when the death condition is found, and pathogen separation inspection is carried out; feeding was continued for another 2 weeks.
The result shows that when the feed obtained in example 1 is fed, the growth speed of tilapia is high, the feed coefficient is low, the survival rate is high, and the feed is beneficial to preventing streptococcus agalactiae.
TABLE 1 growth of Tilapia mossambica after feeding different feeds
Application example 2
2 ponds, pond area are 5 mu, and the density of stocking is 1500 tails/mu, and each pond is established oxygen-increasing machine and feeder respectively 1, and the initial specification of experimental fish 100 + -15 g/tail, two ponds do different treatment respectively:
processing one: feeding special feed for the Haibao dew tilapia;
and (5) processing: the feed prepared in example 2 was fed.
In the culture process, the temperature is 26-32 ℃,1 aerator and 1 feeder are arranged for each treatment, the feeding is carried out for 2 times every day, the time is respectively 8 in the morning and 30 in the afternoon, and the feeding amount is 3% -5% of the fish weight; feeding for 3 months from the first 6 months to the end of 8 months; in the culture process, the eating and disease conditions of the fishes are observed every day, the dead fishes are found, and the fishes are timely fished and counted. Stopping feeding for 1d before detection, measuring the growth of the fish, counting the number of diseases and deaths,
the test results are shown in table 2: the tilapia fed with the feed of the embodiment 2 of the invention has higher growth speed and survival rate, improves the streptococcicosis resistance, reduces the feed coefficient and improves the overall economic benefit.
TABLE 2 growth of Tilapia mossambica after feeding different feeds
Application example 3
2 ponds, the pond area is 3 mu, and the density of stocking is 1300 tails/mu, and each pond is established oxygen-increasing machine and feeder respectively 1, and the initial specification of experimental fish is 200 + -25 g/tail, and two ponds do different treatment respectively:
processing one: feeding special feed for the tilapia with the Haibao sauce;
and (5) processing: the feed of example 3 was fed.
The experiment is from the middle of 6 months to the bottom of 8 months, and the culture experiment lasts for 10 weeks; in the culture process, feeding is carried out for 2 times every day, the feeding time is 8 in the morning and is 30 in the afternoon, the feeding amount is 3% -5% of the weight of the fish, the eating and disease conditions of the fish are observed every day, the dead fish is found, and the number is timely salvaged and counted.
And (4) stopping feeding for 1d before the test is finished, sampling the tilapia, and measuring the growth index of 30 tails/piece. In addition, 10 tails/piece of tilapia are extracted, and serum is extracted to detect the antioxidant index and the activity of lysozyme. The tail vein is used for blood drawing, and the operation is slow. Placing the blood in 1.5ml EP tube, standing in refrigerator at 4 deg.C for 4 hr, centrifuging (4000 r/min,10 min), collecting supernatant, and packaging in 1.5ml EP tube, and storing at-80 deg.C for use.
The activities of total antioxidant capacity (T-AOC), superoxide dismutase (SOD), catalase (CAT) and Lysozyme (LZM) in serum and the content of Malondialdehyde (MDA) are measured by adopting a kit of Nanjing institute of bioengineering (the specific test method is shown in the kit specification).
After 10 weeks of feeding, growth was detected and the number of diseases and deaths occurred was counted, the results are shown in Table 3. The result shows that the compound feed has higher growth speed and survival rate and improves the disease resistance when being fed.
TABLE 3 growth of Tilapia mossambica after feeding different feeds
Application example 4
3 ponds are provided, the area is 3 mu/mu, the stocking density of tilapia is 1300 tails/mu, the specification of tilapia is 210 +/-30 g, and each pond is respectively treated differently:
processing one: feeding special feed for the Haibao dew tilapia;
and (5) processing: feeding the feed special for the Baiyang tilapia;
and (3) treatment III: the feed of example 4 of the present application was fed.
Feeding twice a day, namely 8 am and 17 pm, wherein the feeding amount is 3-5% of the weight of the fish, and is adjusted according to specific conditions; the test time is 8 weeks, and the test is finished from the first 7 months to the end of 8 months; stopping feeding for 1d before blood sampling, collecting tilapia blood by adopting a tail vein blood sampling mode, standing for 4h in a refrigerator at 4 ℃, centrifuging and collecting supernatant, and storing in the refrigerator for later use. Measuring total antioxidant capacity (T-AOC), superoxide dismutase (SOD), lysozyme (LZM) and Malondialdehyde (MDA), and determining with Nanjing institute of bioengineering kit (the specific test method is described in the kit description).
The test results are shown in Table 4, and the total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and Malondialdehyde (MDA) content of Lysozyme (LZM) of tilapia serum fed with the feed of the example 4 are all higher than that of a commercial feed C and that of a commercial feed D; while Malondialdehyde (MDA) is low. It can be seen that feeding tilapia with the feed of example 4 helps to increase the growth rate and improve the antioxidant and disease resistance of tilapia.
TABLE 4 Pond Tilapia culture test results
According to the embodiment, the feed provided by the invention does not contain antibiotic components, so that the capacity of preventing streptococcal diseases of tilapia can be improved, the growth of the tilapia can be accelerated, the immunity and disease resistance of the tilapia can be improved, the feed containing antibiotic medicines and used for preventing streptococcal diseases of the tilapia can be replaced, and the use of the antibiotic medicines in the feed for preventing streptococcal diseases of the tilapia is reduced.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and all of the embodiments are included in the scope of the present invention.
Claims (7)
1. The feed additive for preventing the streptococcicosis of tilapia is characterized by comprising the following components in parts by mass: 5 to 20 portions of zymosan, 10 to 20 portions of mint leaves, 10 portions of wrinkled gianthyssop herb, 10 to 20 portions of mulberry leaves, 10 to 20 portions of heartleaf houttuynia herb, 5 to 10 portions of common lophatherum herb, 10 to 20 portions of Chinese violet, 5 portions of liquorice and 5 portions of tuckahoe.
2. The feed for preventing streptococcicosis of tilapia is characterized by being prepared from the following raw materials in parts by mass: 84 to 116 parts of basal feed and 2 to 6 parts of feed additive as claimed in claim 1.
3. The feed according to claim 2, wherein the basal feed comprises the following components in parts by mass: 30 to 35 portions of protein, 4 to 8 portions of fat, 0.6 to 1.0 portion of calcium, 0.8 to 1.2 portions of phosphorus, 1.5 to 2.0 portions of lysine and 0.3 to 0.6 portion of methionine.
4. The feed as claimed in claim 2 or 3, wherein the basal feed is prepared from the following raw materials in parts by mass: 3 to 6 portions of fish meal, 4 to 8 portions of chicken meal, 35 to 40 portions of bean pulp, 15 to 20 portions of peanut bran and 1 portion of corn bran0 to 17 portions of cassava starch, 10 to 15 portions of cassava starch, 3 to 5 portions of soybean oil, 0.5 portion of salt, 0.5 portion of choline chloride and Ca (H) 2 PO 4 ) 2 1-2 parts and premix 2233#2 parts of omnivorous fish.
5. A process for preparing the feed of any one of claims 2 to 4, comprising the steps of:
(1) Mixing fish powder, chicken powder, soybean meal, peanut bran, corn bran, cassava starch, salt, omnivorous fish premix 2233#, choline chloride, and Ca (H) 2 PO 4 ) 2 Mixing with feed additive to obtain pre-mixed feed;
(2) And spraying soybean oil on the pre-mixed feed to obtain the feed for preventing the streptococcal disease of the tilapia.
6. The method of claim 5, wherein in step (1), the fish meal, chicken meal, soybean meal, peanut bran, corn bran, tapioca starch, salt, omnivorous fish premix 2233#, choline chloride, ca (H) are mixed with the feed of step (1) 2 PO 4 ) 2 Mixing with feed additive, and sieving;
and (3) before spraying the soybean oil, sequentially crushing the pre-mixed feed, removing impurities, mixing for the second time, puffing the feed and drying.
7. The method of claim 6, wherein the pulverization is ultrafine pulverization; the aperture size of the sieve is 80 meshes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911213405.2A CN110959773B (en) | 2019-12-02 | 2019-12-02 | Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911213405.2A CN110959773B (en) | 2019-12-02 | 2019-12-02 | Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110959773A CN110959773A (en) | 2020-04-07 |
| CN110959773B true CN110959773B (en) | 2023-03-24 |
Family
ID=70032577
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911213405.2A Active CN110959773B (en) | 2019-12-02 | 2019-12-02 | Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110959773B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113826780A (en) * | 2021-10-15 | 2021-12-24 | 永辰水产科技(盐城)有限公司 | Chinese herbal medicine feed additive for promoting growth of micropterus salmoides and improving immunity |
| CN113826781A (en) * | 2021-10-15 | 2021-12-24 | 永辰水产科技(盐城)有限公司 | Feed additive for preventing and treating largemouth black bass ulcer syndrome |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110886A (en) * | 2013-01-21 | 2013-05-22 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting streptococcus |
| CN103250875A (en) * | 2013-06-08 | 2013-08-21 | 何兴江 | Special compound feed for tilapia |
| CN103330855A (en) * | 2013-07-02 | 2013-10-02 | 镇江天和生物技术有限公司 | Medicine preparation for preventing hemorrhagic disease of grass carp |
| CN103734538A (en) * | 2014-01-09 | 2014-04-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of feed capable of protecting liver of GIFT oreochromis niloticus and boosting immunity of GIFT oreochromis niloticus and feeding method of feed |
| CN104381343A (en) * | 2013-09-17 | 2015-03-04 | 闽南师范大学 | Water-soluble compound antibacterial agent and preparation method thereof |
| CN105341352A (en) * | 2015-12-15 | 2016-02-24 | 李国深 | Feed additive for preventing streptococcus agalactiae |
| CN106389683A (en) * | 2016-12-18 | 2017-02-15 | 长沙瑞多康生物科技有限公司 | Composition capable of preventing fish streptococcicosis |
| CN106615993A (en) * | 2016-09-13 | 2017-05-10 | 通威股份有限公司 | Compound feed used for tilapia mossambica breeding and preparation method thereof |
| CN109329655A (en) * | 2018-11-20 | 2019-02-15 | 张项春 | The mixed feed for preventing Tilapia mossambica streptococcosis |
| CN110432404A (en) * | 2019-09-03 | 2019-11-12 | 中国水产科学研究院淡水渔业研究中心 | Reduce the tilapia feed additive and its preparation method and application of proinflammatory response with enhancing immune response |
-
2019
- 2019-12-02 CN CN201911213405.2A patent/CN110959773B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103110886A (en) * | 2013-01-21 | 2013-05-22 | 宁波大学 | Traditional Chinese medicinal recipe for inhibiting streptococcus |
| CN103250875A (en) * | 2013-06-08 | 2013-08-21 | 何兴江 | Special compound feed for tilapia |
| CN103330855A (en) * | 2013-07-02 | 2013-10-02 | 镇江天和生物技术有限公司 | Medicine preparation for preventing hemorrhagic disease of grass carp |
| CN104381343A (en) * | 2013-09-17 | 2015-03-04 | 闽南师范大学 | Water-soluble compound antibacterial agent and preparation method thereof |
| CN103734538A (en) * | 2014-01-09 | 2014-04-23 | 中国水产科学研究院淡水渔业研究中心 | Preparation method of feed capable of protecting liver of GIFT oreochromis niloticus and boosting immunity of GIFT oreochromis niloticus and feeding method of feed |
| CN105341352A (en) * | 2015-12-15 | 2016-02-24 | 李国深 | Feed additive for preventing streptococcus agalactiae |
| CN106615993A (en) * | 2016-09-13 | 2017-05-10 | 通威股份有限公司 | Compound feed used for tilapia mossambica breeding and preparation method thereof |
| CN106389683A (en) * | 2016-12-18 | 2017-02-15 | 长沙瑞多康生物科技有限公司 | Composition capable of preventing fish streptococcicosis |
| CN109329655A (en) * | 2018-11-20 | 2019-02-15 | 张项春 | The mixed feed for preventing Tilapia mossambica streptococcosis |
| CN110432404A (en) * | 2019-09-03 | 2019-11-12 | 中国水产科学研究院淡水渔业研究中心 | Reduce the tilapia feed additive and its preparation method and application of proinflammatory response with enhancing immune response |
Non-Patent Citations (2)
| Title |
|---|
| 中药药理与应用;冯彬彬;《中药药理与应用》;中药中医药出版社;20151130;第123-124页 * |
| 罗非鱼链球菌的浓度测定方法的确定及体外抑菌研究;颜东亮等;《北方药学》;CNKI;20120430;第9卷(第4期);摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110959773A (en) | 2020-04-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104799124B (en) | A kind of colored clam mixed feed | |
| JP2018536394A (en) | Artificial feed for bat larvae and method for preparing the same | |
| CN102987121A (en) | Piglet feed capable of strengthening immunity and preparation method of piglet feed | |
| CN110959773B (en) | Feed additive and feed for preventing streptococcicosis of tilapia mossambica, and preparation method and application of feed additive and feed | |
| CN103636990B (en) | A kind of green pig's feed that improves efficiency of feed utilization | |
| CN102940140A (en) | Green health goose forage and preparation method thereof | |
| KR100908738B1 (en) | A method for extracting β-glucan of Astragalus and feed composition comprising the β-glucan | |
| CN102696901B (en) | Feed for meat-producing chick | |
| CN113632894A (en) | Ecological fish feed mainly prepared from fermented ramulus mori as well as preparation method and application of ecological fish feed | |
| CN102511690A (en) | Feed formula for resisting heat stress of broilers | |
| CN103535537A (en) | Method for preparing feed for culturing adult river crab | |
| CN108041345B (en) | Disease-resistant growth-promoting maggot protein feed additive for adult American eels and preparation method and application thereof | |
| KR101542882B1 (en) | A composition for prevention and treatment of fish viral hemorrhagic septicemia containing extracts of Ecklonia cava | |
| CN104273345B (en) | A kind of Macrobrachium rosenbergii composite immune reinforcing agent and its application | |
| CN102987066A (en) | Preparation method of modified peanut straw pig feed and prepared pig feed | |
| CN103750069B (en) | Feed additive for prevention and treatment of fish bacteria and virus diseases | |
| CN108522819A (en) | A kind of compound pig feed and preparation method thereof for improving immunity, promoting growth | |
| AU2020104013A4 (en) | Functional Feed Composition for Laying Ducks and Preparation Method Thereof | |
| EP3536162A1 (en) | Feed and method for manufacturing same | |
| CN107772061A (en) | A kind of immunity enhancement type compound feed for Litopenaeus vannamei and preparation method thereof | |
| CN106615922A (en) | Feed for reducing attack rate of inclusion body hepatitis of broilers | |
| KR101273276B1 (en) | Method for producing feed for livestock adding curcumin and feed for livestock produced by the same | |
| CN1795743A (en) | Proportioned feedstuff for chickens | |
| KR20120080793A (en) | Feed composition comprising chestnut and manufacturing method thereof | |
| JP2011241192A (en) | Disease controlling agent for crustacean, and feed containing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |