KR101273276B1 - Method for producing feed for livestock adding curcumin and feed for livestock produced by the same - Google Patents

Abstract

The present invention relates to a method for producing a livestock feed, and to a method for producing a livestock feed and a livestock feed prepared by the above method, characterized in that by adding curcumin (curcumin) to the livestock feed, More specifically, by feeding the animal feed curcumin added to the livestock, it improves the growth rate and immunity of the livestock, and improves the pH, water activity of the livestock meat after the slaughter and the heat loss during heating the livestock meat Genie relates to a method for producing livestock feed and to livestock feed produced by the above method.

Description

Method for producing livestock feed containing curcumin and feed for livestock produced by the above method {Method for producing feed for livestock adding curcumin and feed for livestock produced by the same}

The present invention relates to a method for producing a livestock feed, and to a method for producing a livestock feed and a livestock feed prepared by the above method, characterized in that by adding curcumin (curcumin) to the livestock feed, More specifically, by feeding the animal feed curcumin added to the livestock, it improves the growth rate and immunity of the livestock, and improves the pH, water activity of the livestock meat after the slaughter and the heat loss during heating the livestock meat Genie relates to a method for producing livestock feed and to livestock feed produced by the above method.

Curcumin (Turmetic, Curcumin Longa L) is a plant polyphenolic drug that is a perennial of ginger family, and its origin is Asian in India, China, and Japan, and is widely used for herbal medicine, spices, and food. Curcumin is native to hot and humid southern Asia, Africa, and Latin America, and has been cultivated in East India. It is known as turmeric, gallium and turmeric as a foreign plant transmitted to Buddhism in Korea.

Curcumin has been reported to inhibit platelet aggregation not only in vivo but also in vitro, and has anti-oxidant, anti-viral, anti-inflammatory, anti-cancer, anti-lipid and anti-inflammatory effects, anti-angiogenic and anti-angiogenic effects associated with cancer cell proliferation. Anti-inflammatory, antioxidant, anticancer, and antibiotics of curcumin have been reported as a β-lactam antibiotic that suppresses squalene synthase, which is involved in the biosynthesis of cholesterol, and lowers the level of cholesterol accumulated in the liver, which is effective in the liver and nervous system. Many studies are underway.

As consumers' income increases, demand for various livestock products, including beef and pork, is rapidly increasing in response to changes in eating habits. In order to keep up with these demands, to provide good quality livestock products, it is of utmost importance to provide good quality livestock products by preventing animals from becoming diseased or by improving the meat quality before slaughter. In addition, as the hog industry has become larger, it is common to use antibiotics or chemical treatments as feed additives to improve productivity and improve meat quality. However, long-term use of antibiotics or chemotherapy in pigs can lead to antibiotic retention or resistance issues in the product, which is why regulations on antibiotic use are tightening worldwide. Therefore, there is a need for a feed that improves meat quality and promotes growth without using antibiotics.

Korean Patent Publication No. 2010-0056064 discloses a functional feed containing mulberry leaves and a manufacturing method thereof, and Korean Patent Publication No. 2004-0090017 discloses a compounded feed composition for livestock and a method of preparing the same. It is different from the manufacturing method of the feed for livestock containing curcumin as an active ingredient.

The present invention is derived from the above requirements, in the present invention by feeding the animal feed curcumin (curcumin) added to the livestock, by improving the growth rate and immunity of the livestock to improve the productivity of the livestock (curcumin) It can be used as a substitute for antibiotics, and curcumin, which has the effect of improving the quality of meat and providing high quality livestock meat by improving the pH, water activity and heating loss of livestock meat after slaughter, The purpose is to provide livestock feed.

In order to solve the above problems, the present invention provides a method for producing a livestock feed, characterized in that the curcumin (curcumin) is added to the feed for the livestock.

The present invention also provides a feed for livestock produced by the above method.

In addition, the present invention contains a curcumin (curcumin) as an active ingredient, improves the growth rate and immunity of the livestock, and provides a feed composition for livestock feed for improving the pH, water activity and heating loss of the livestock meat heating after slaughter do.

According to the present invention, by adding curcumin (curcumin) to the feed for livestock feed to the livestock, improve the growth rate and immunity of the livestock to improve the productivity of the livestock and curcumin (curcumin) can be used as an antibiotic replacement material antibiotics It is possible to reduce livestock mortality without using it, and to improve meat quality and provide high quality livestock meat by improving the pH, water activity and heating loss of livestock meat after slaughter.

Figure 1 shows the blood collection time to investigate the IgG content of weaning pigs, cholera + pigs alone vaccine is administered at the 2nd and 4th week of the experiment, respectively, the first blood collection 3 days before the 2nd week vaccine injection, the first vaccine administration After two weeks, blood was drawn again, and two weeks after the second vaccine administration, the blood was drawn again.
Figure 2 shows the contents of Lactobacillus and E. coli microorganisms in the ileum after weaning piglet slaughter.
T1: Chairman of weaning pigs fed antibiotic-free feed
T2: The ileum of weaning pigs fed a feed with 0.2% antibiotics
T3: Chairman of weaning pigs fed a diet supplemented with curcumin 0.02%
T4: Chairman of weaning pigs fed a diet supplemented with curcumin 0.04%
T5: Chairman of weaning pigs fed a diet supplemented with curcumin 0.06%
Figure 3 shows the IgG content three days before (white graph), two weeks after the administration (dashed graph) and four weeks (assay graph) of the vaccine (vaccine) to investigate the immune response of the weaning pigs fed curcumin will be.
Comparative Example 1: IgG content of weaned pig serum fed antibiotic-free feed
Comparative Example 2: IgG content of weaned pig serum fed diets added with 0.2% antibiotics
Example 1 IgG Content of Weaned Pig Serum Fed Feeds Added with Curcumin 0.02%
Example 2 IgG Content of Weaned Pig Serum Fed Feeds Added with Curcumin 0.04%
Example 3: IgG content of weaned pig serum fed feed added with curcumin 0.06%

In order to achieve the object of the present invention, the present invention provides a method for producing a livestock feed, characterized in that by adding curcumin (curcumin) to the feed for the livestock feed.

In the method of the present invention, the livestock may be a pig, but is not limited thereto.

In addition, in the method of the present invention, the curcumin (curcumin) may be extracted from turmeric, the extraction method is

(a) crushing and washing turmeric and extracting 1-3 times with 70-90% methyl alcohol for 50-70 minutes;

(b) cooling the extracted turmeric, and centrifuging and filtering to obtain only a solution;

(c) dissolving the solution of step (b) with 70-90% methyl alcohol and concentrating in a vacuum at 40-60 ° C .; And

(d) drying and sterilizing the concentrated solution of step (c) for 15-20 seconds, and may include preparing a powder,

Preferably

(a) crushing and washing turmeric and extracting twice with 80% methyl alcohol for 60 minutes;

(b) cooling the extracted turmeric, and centrifuging and filtering to obtain only a solution;

(c) dissolving the solution of step (b) with 80% methyl alcohol and concentrating in a vacuum at 50 ° C .; And

(d) drying and sterilizing the concentrated solution of step (c) for 15 to 20 seconds and then preparing a powder, but is not limited thereto.

The purity of curcumin extracted from the turmeric may be 90 to 100%, preferably 95%, but is not limited thereto.

In addition, in the method of the present invention, by feeding the animal feed curcumin (curcumin) added to the livestock, thereby improving the growth rate and immunity of the livestock, the pH, water activity of the livestock meat after slaughter and heating loss when heating the livestock meat This is characterized by being improved, but is not limited thereto.

Specifically, the method of the present invention, when the livestock is a pig,

(a) crushing and washing turmeric and extracting 1-3 times with 70-90% methyl alcohol for 50-70 minutes;

(b) cooling the extracted turmeric, and centrifuging and filtering to obtain only a solution;

(c) dissolving the solution of step (b) with 70-90% methyl alcohol and concentrating in a vacuum at 40-60 ° C .;

(d) drying and sterilizing the concentrated solution of step (c) for 15 to 20 seconds to prepare a powder to obtain curcumin; And

(e) adding the curcumin (curcumin) of step (d) to the pig feed 0.01 to 0.03% by weight,

More specifically

(a) crushing and washing turmeric and extracting twice with 80% methyl alcohol for 60 minutes;

(b) cooling the extracted turmeric, and centrifuging and filtering to obtain only a solution;

(c) dissolving the solution of step (b) with 80% methyl alcohol and concentrating in a vacuum at 50 ° C .;

(d) drying and sterilizing the concentrated solution of step (c) for 15 to 20 seconds to prepare a powder to obtain curcumin; And

(e) adding the curcumin (curcumin) of step (d) to the pig feed 0.02% by weight.

The present invention also provides a feed prepared by the above method.

The present invention also provides curcumin (curcumin) as an active ingredient, improves the growth rate and immunity of the livestock, and provides a feed composition for livestock feed for improving the pH, water activity and heating loss of the livestock meat heating after slaughter do. The composition is preferably a pig feed composition.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are illustrative of the present invention, and the present invention is not limited to the following examples.

One. From turmeric Curcumin  How to extract

The harvested turmeric was cut into small pieces of 0.5 ~ 1 cm thickness, washed three times in clean water, and extracted twice with 80% methyl alcohol for 1 hour. The extracted solution was cooled and then centrifuged at 4 ° C. and filtered to separate only the solution. The solution was again dissolved in 80% methyl alcohol, separated, and concentrated in vacuo at 50 ° C. The concentrated material was analyzed by Curcumin content using HPLC, and after analysis, it was dried and sterilized for 15-20 seconds at high pressure and high temperature, and then passed through an 80 mm mesh screen to make powder, and finally the Curcumin content was extracted to 95%. .

2. In reason  About Curcumin  Pay effect

(1) Experimental method

In order to investigate the effect of curcumin antibiotic replacement, 100 pigs were weaned. Treatment groups were T1 (no antibiotics, basal diet), T2 (0.2% antibiotics), T3 (0.02% turmeric extract), T4 (0.04% turmeric extract), T5 (0.06% turmeric extract) Divided by 5 replicates and 4 randomly placed per replicate. The nutrient level of the test feed was in accordance with the recommended amount according to the NRC specification standard (1998), and the composition of the test feed is shown in Table 1. The test period was performed for 6 weeks after weaning. The experimental feed Phase 1 was fed for 2 weeks after the start of the experiment. The experimental feed Phase 2 was fed from 2 weeks to 6 weeks after the start of the experiment. The specification management was carried out in accordance with the practice of the National Institute of Animal Science and Pigs.

Chemical Composition of Weaned Pig Experiment Ingredients (%) Phase 1 Phase 2 Bakery BP 5 5 Expanded Corn 44.06 59.505 Soybean Meal 20 24 Pepsoygen 4.35 - Fishmeal 5 2.5 Soy oil 406 1.55 Limestone 0.3 0.5 TCP (18%) 0.1 0.5 Salt (Flower salt) 0.3 0.3 Sweetener 0.05 0.05 Whey 15 5 DL-Met 0.19 0.175 L-Lysine-HCL 0.5 0.4 Throneine 0.13 0.14 Choline Chrolide 50% 0.1 0.08 Vitamin mixture 0.12 0.1 Mineral mixture 0.2 0.2 Total 100 100

In this study, MS-typing was performed before the start of the test in order to reduce the genetic difference between the donated pigs, and blood collection was performed before and after the administration of the cholera + don alone vaccine to investigate the immune response to curcumin. Was carried out.

3. for hogs Curcumin  Pay effect

(1) Experimental method

To investigate the effect of curcumin on the rearing pigs, 30 pigs were raised. Treatments were divided into T1 (antibiotic-free, basal diet), T2 (vitamin E added), T3 (curcumin 200 ppm added), repeated 10 times, and randomly placed two heads per replicate. The nutrient level of the experimental feed was in accordance with the recommended amount according to the NRC specification standard (1998), and the composition of the specific test feed is shown in Table 2. The test period was performed for 12 weeks. The experimental feeding phase was fed for 6 weeks after the start of the experiment. Specification management was carried out in accordance with the practice of the National Institute of Animal Science and Pigs.

Chemical Composition of Experimental Feeders Ingredient Growing phase Finishing phase Corn 67.32 74.93 Soybean meal 25.00 15.73 Wheat bran 2.50 6.07 Soy oil 1.50 1.66 L-lysine 0.08 0.17 Salt 0.30 0.20 Calcium phosphate 2.00 0.20 Limestone 0.70 0.81 Vitamin-mineral mix 0.60 0.23 Total 100 100

Experimental items were examined for daily weight gain, feed intake, feed demand rate, and carcass characteristics and quality of meat after slaughter.

Example  One: Curcumin  According to the added feed Weaning  growth

The weight, daily weight gain, daily feed intake and feed requirement of the weaning pigs according to the curcumin added amount of the feed are shown in Table 3 below. As a result, curcumin added 0.02% higher daily gain than the non-antibiotic treatment.

Growth of Weaning Pigs by Feeding Curcumin Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 SE weight
(kg) Starting weight 8.55 8.63 8.94 9.09 8.91 0.015 Weight 32.65 33.34 33.41 32.83 31.73 0.014 One day
Weight gain
(g / day) 0-21 days 439 458 456 435 417 0.024 21-42 days 709 719 709 696 670 0.031 0-42 days 574 588 582 565 543 0.026 Feed per day
Intake
(kg / day) 0-21 days 0.774 0.651 0.659 0.631 0.607 0.024 21-42 days 1.424 1.252 1.277 1.257 1.199 0.031 0-42 days 1.100 0.951 0.968 0.944 0.903 0.026 feed
Demand rate
0-21 days 1.64 1.48 1.45 1.58 1.45 0.045 21-42 days 1.87 1.76 1.79 1.87 1.80 0.022 0-42 days 1.77 1.65 1.66 1.76 1.66 0.024

Comparative Example 1: Weaned pigs fed antibiotic-free feed

Comparative Example 2: Weaned pigs fed the feed with 0.2% antibiotics

Example 1: Weaned pigs fed dietary supplement with curcumin 0.02%

Example 2: Weaned pigs fed dietary supplement with curcumin 0.04%

Example 3: Weaned pigs fed dietary supplement with curcumin 0.06%

Example  2: Curcumin  According to the added feed Weaning pigs  Microbial content of the ileum

Type yiyujadon the experiment for 6 weeks and were collected by the President of the contents slaughter dussik 3 per treatment group, results meeting Lactobacillus bacteria (Lactobacillus) and E. coli irradiating microorganisms (E. Coli) did not show a significant difference in the treatments (2 ).

Example  3: Curcumin  According to the added feed Weaning  Immune response

In order to investigate the immune response, the change in T cells before, 2 and 4 weeks after vaccine was measured, and T3 treatment (weaned pig serum fed the diet supplemented with curcumin 0.02%) was given antibiotic-free feed. The content of IgG was found to be highest compared to the spheres (FIG. 3).

Example  4: Curcumin  According to the added feed Of rearing pigs  growth

The daily weight gain, daily feed intake and feed demand of feeders fed curcumin-added feed are shown in Table 4 below. As a result, non-antibiotic feed and vitamin E The daily diet gain was higher in Curcumin 200 ppm supplemented diet than the supplemented diet.

Growth of Fostered Pigs with Curcumin-Added Feed Comparative Example 1 (Antibiotics) Example 1 (vitamin E) Example 2 (curcumin 200 ppm) Daily weight gain (g) 911 (100) 962 (106) 975 (107) Feed Intake (kg) 2.58 2.59 2.70 Feed rate 2.83 2.69 2.78

Example  5: Curcumin  According to the added feed Of rearing pigs  Conductor properties

The pH, carcass weight, backfat thickness, flesh color, and marbling of the fed carcasses fed curcumin-added feed were measured 40 minutes after slaughter and are shown in Table 5. As a result, when the conductor was oxidized, the pH dropped, but the pH of the curcumin 200 ppm addition sphere was higher than that of the comparative example, and the oxidation of the conductor proceeded slowly by the addition of curcumin. And marbling did not show a big difference between the comparative example and the Example.

Carcass Characteristics of Fostered Pigs with Curcumin Supplementation Comparative Example 1 (Antibiotics) Example 1 (vitamin E) Example 2 (curcumin 200 ppm) pH 6.04 6.18 6.21 Conductor weight (kg) 82.11 84.40 85.40 Back fat thickness (mm) 22.67 23.70 24.30 Color 4.44 4.20 4.20 Marbling 2.22 2.10 2.20

Example  6: Curcumin  Physicochemical Characteristics of Pork According to Added Feed Supplementation

The color, heating loss, shear force and water activity of pork fed curcumin-added feed are shown in Table 6. As a result, the heat loss of pork fed the curcumin-containing feed decreased and the water activity was higher than that of the comparative example.

Physicochemical Characteristics of Pork According to the Dietary Supplement with Curcumin Physicochemical properties Comparative Example 1 (Antibiotics) Example 1 (vitamin E) Example 2 (curcumin 200 ppm) pH 5.67 5.68 5.67
CIE
L 50.85 52.05 51.08 a 5.57 5.46 5.82 b 3.61 3.74 3.60
Hunter
L 43.77 44.95 44.00 a 4.53 4.37 4.74 b 2.78 2.92 2.78 Heating loss 33.01 31.94 31.64 Shear force 3.15 3.13 3.14 Water activity 56.39 57.88 57.01

Example  7: Curcumin  General Components of Pork According to Added Feed Supplementation

The moisture, fat, protein and ash contents of pork fed curcumin-added feed are shown in Table 7. As a result, the dietary supplement with curcumin did not show a significant effect on the general composition of pork.

Common Ingredients of Pork Comparative Example 1 (Antibiotics) Example 1 (vitamin E) Example 2 (curcumin 200 ppm) Moisture content 75.39 75.08 75.21 Fat content 2.81 2.75 2.75 Protein content 23.46 23.76 23.68 Ash content 1.04 1.03 1.02

Claims (8)

delete delete delete delete delete (a) crushing and washing turmeric and extracting 1-3 times with 70-90% methyl alcohol for 50-70 minutes;
(b) cooling the extracted turmeric, and centrifuging and filtering to obtain only a solution;
(c) dissolving the solution of step (b) with 70-90% methyl alcohol and concentrating in a vacuum at 40-60 ° C .;
(d) drying and sterilizing the concentrated solution of step (c) for 15 to 20 seconds to prepare a powder to obtain curcumin; And
(e) improving the growth rate and immunity of pigs, characterized in that the curcumin (curcumin) of step (d) is added to the pig feed 0.01 ~ 0.03% by weight, pork, pH, water activity and pigs after slaughter Method of producing a pig feed that loses heat when meat is heated. Pig feed prepared by the method of claim 6. delete

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