KR101032033B1 - Antimicrobial composition comprising medium chain fatty acids for addition to feedstuff, process thereof and feedstuff comprising the same - Google Patents

Abstract

The present invention relates to an antimicrobial composition for adding livestock feed containing a medium chain fatty acid, a method for preparing the same, and a livestock feed including the antimicrobial composition. More specifically, the present invention relates to an antimicrobial activity equivalent to that of antibiotics based on natural materials such as yucca extract. To suppress various bacterial and metabolic diseases of livestock, improve the nutrients and energy supply and usability to increase livestock productivity and productivity, including antimicrobial composition for adding livestock feed, its manufacturing method and the antimicrobial composition It is about livestock feed.

The antimicrobial composition for livestock feed containing the medium chain fatty acid according to the present invention significantly increases the growth and feed intake of weaning piglets when used in feed, and increases the productivity of weaning piglets than the zinc oxide addition group as well as antibiotics for promoting growth. It was very effective in improving the quality of the animal, and excellent productivity could be obtained by replacing antibiotics and zinc oxide in the weaning pig section.

Medium chain fatty acid, yucca extract, livestock feed, antimicrobial composition, zero coli extract

Description

ANTIMICROBIAL COMPOSITION COMPRISING MEDIUM CHAIN FATTY ACIDS FOR ADDITION TO FEEDSTUFF, PROCESS THEREOF AND FEEDSTUFF COMPRISING THE SAME}

The present invention relates to an antimicrobial composition for adding livestock feed containing a medium chain fatty acid, a method for preparing the same, and a livestock feed including the antimicrobial composition. More specifically, the present invention relates to an antimicrobial activity equivalent to that of antibiotics based on natural materials such as yucca extract. To suppress various bacterial and metabolic diseases of livestock, improve the nutrients and energy supply and usability to increase livestock productivity and productivity, including antimicrobial composition for adding livestock feed, its manufacturing method and the antimicrobial composition It is about livestock feed.

In the livestock industry, despite the increasing demand for meat as the population grows and industry develops, the production costs continue to rise due to inflation, such as feed, labor, and farming facilities, and plateau oil prices. The environment is on the rise.

In addition, domestic livestock industry is expected to weaken the competitiveness of domestic livestock products due to chronic diseases and future free trade (FTA). For example, from 2009, the use of 7 antibiotics in feeds will be further regulated. Vulnerability and reduced productivity are a concern throughout the domestic livestock industry.

Therefore, there is an urgent need to find antibiotic substitutes for livestock feed to ensure productivity that can respond to regulations on the use of antibiotics for promoting growth in feeds, and to satisfy fundamental and essential factors such as the certainty of efficacy of chemotherapy antimicrobials and the safety of products. .

Yucca is a type of cactus that grows in desert areas from the southern United States to Central America. Native Americans have long used yucca for healing and health purposes. They also cooked yucca in a variety of ways, including flowers, berries, seeds, stems, and roots. For medical purposes, Yucca has been used effectively to treat rheumatism and arthritis, and the stems of the yucca are cut and boiled with water to make a liquid concentrate, which is used by arthritis patients or applied to joints. This method has been proven in modern science and is still used in many countries around the world to treat arthritis.

Yucca has been used since the American Indian era for the treatment of rheumatoid and arthritis or as a spice in food. To be extracted sap of yucca make the tablets to make a syrup or dried into a powder, the US FDA in was allowed as a product to be able to use Majave yucca extract zero natural flavor of syrup or tablet form, Yucca schidigera and Yucca brevifolia FIG food Licensed as a flavoring agent. Recently, it is used to make bubbles of root beer, which is a soft drink, and is also used in soaps and shampoos.

Yucca has been applied to pharmaceuticals as a raw material of soap or steroid hormones using foaming up to the 1950s, and began to be applied to the livestock industry in the 1960s. In the 1970s, the scope of application has been extended to new fields such as improving the livestock environment and livestock growth.

The most important factor that indicates the physiological activity of yucca is Sarsaponin, which is contained in a large amount in yucca, and saponin is known to be contained in ginseng and red ginseng.

The saponin component of yucca shows an excellent effect as a deodorant. Although the mechanism of removing the odor is not clear yet, the mechanism of action is suggested because the saponin component of the yucca extract is directly bonded to the odor component. When the yucca extract was added to a solution containing odor-causing substances such as dimethyl sulfide, indole and skatol, the degree of odor was greatly improved. In this regard, the Selma City Sanitation Bureau of California, USA, used yucca extracts to examine the degree of organic decomposition and odor reduction of activated sludge, and reported that the amount of hydrogen sulfide released into the atmosphere was greatly reduced.

In the present invention, the main ingredient is the yucca extract, which is a natural substance, and blends heavy chain fatty acid and essential oil to produce an antimicrobial composition for animal feed additives. As a result of feeding the livestock such as beef cattle and feed to the livestock, the present invention was completed by investigating feed efficiency, antibacterial effect, weight gain effect, odor reduction effect, and the like.

It is an object of the present invention to provide an antimicrobial composition for livestock feed addition, which is composed mainly of yucca extract, which is a natural substance that can replace chemotherapy, and heavy chain fatty acids.

It is also an object of the present invention to provide a method for preparing an antimicrobial composition for livestock feed, comprising Yucca extract and medium-chain fatty acids as main components.

Another object of the present invention is to provide a livestock feed having excellent antimicrobial activity, including an antimicrobial composition for the addition of livestock feed containing mainly yucca extract and medium chain fatty acids.

Hereinafter, the present invention will be described in detail.

The present invention is 5 to 10% by weight of yucca extract, 5 to 10% by weight of medium chain fatty acid, 5 to 10% by weight of essential oil, 20 to 25% by weight of vegetable fat, chitin or chitosan fermentation 5 to 100% by weight of the total composition. 10% by weight, yeast culture 12-15%, calcium carbonate 12-15%, chaff powder 16-20% by weight and flour 15 to 20% by weight of the antimicrobial composition for animal feed .

Hereinafter, the present invention will be described in more detail.

Antimicrobial composition for livestock feed comprising a medium-chain fatty acid according to the present invention

1) preparing a yucca extract powder whose final moisture content is adjusted to a level of 8% or less;

2) 5 to 10% by weight of the yucca extract powder obtained in step 1), 5 to 10% by weight of at least one medium chain fatty acid selected from the group consisting of capric acid, caprylic acid and capric acid, based on 100% by weight of the total antimicrobial composition. And 5 to 10% by weight of essential oils mixed by grinding the nanopowder;

3) coating the nanopowder by adding 20 to 25 wt% of vegetable fat to 100 wt% of the total antimicrobial composition to the nanopowder obtained in step 2); And

4) 5 to 10% by weight of chitin or chitosan fermentation, 12 to 15% by weight of yeast culture, 12 to 15% by weight of calcium carbonate, based on 100% by weight of the total antimicrobial composition to the nanopowder coated with the vegetable fat obtained in step 3). , 16 to 20% by weight chaff and 15 to 20% by weight of flour is prepared by mixing.

As described above, the antimicrobial composition for livestock feed according to the present invention first pulverizes the yucca and extracts the yucca juice by press compression. The obtained yucca juice is removed by the evaporation process at a temperature of about 100 ℃, dried in a hot air method to prepare a yucca extract in the form of a powder in which the final moisture content is adjusted to 8% or less.

5 to 10% by weight of the yucca extract powder obtained as described above with respect to 100% by weight of the total antimicrobial composition is mixed with 5 to 10% by weight of medium chain fatty acid and 5 to 10% by weight of essential oil, and finely pulverized. In the present invention, the pulverization process preferably comprises after about two hours of reaction in the nanoreactor, each particle pulverizes the product to a particle size of about 100 nanometers on average. By such a nanonized powder process, each component can maximize the reaction surface area can double the antimicrobial activity.

Heavy chain fatty acid among the components included in the composition of the present invention improves the energy metabolism efficiency of livestock, improves the growth of fibrinolytic microorganisms, enhances the production rate of propionic acid in volatile fatty acids (Volatile Fatty Acids, VFA), energy supply Not only does it increase the body's protein accumulation.

Feeding of the heavy chain fatty acid contained in the composition according to the present invention can maintain a stable pH in the rumen of the livestock can significantly lower the risk for acid poisoning (pH <5.5), so the amount of feed is rich compared to the survey as in Korea It may be particularly useful in high cases.

In the present invention, the medium-chain fatty acid acts to lower the risk of metabolic diseases by improving the intestinal health of pigs and beef cattle based on the carcass rate enhancing effect and the strong antibacterial activity through the promotion of pigs and beef cattle. The antimicrobial composition for livestock feed according to the present invention containing a medium chain fatty acid dramatically reduces the production of methane gas in the rumen.

Antimicrobial composition for livestock feed according to the present invention is preferably one selected from the group consisting of capronic acid (Capronic acid (C6)), caprylic acid (Caprylic acid (C8)) and capric acid (Cric acid (C10)) The above medium chain fatty acid is added.

The essential oils used in the present invention include eucalyptus, lemon, grapefruit, lavender extract, and the like. Preferably, zero coli extract can be used.

A mixture of finely divided yucca extract, medium chain fatty acids and essential oils to a nanopowder size is coated with vegetable fat. In the present invention, the mixture comprising the nanonized yucca extract is coated by mixing with vegetable fat in a rotary mixer or coated by spraying vegetable fat in a spray mixer. Such coating treatment of the nanonized powder by vegetable fat can minimize the reaction on the livestock and maximize the reaction force in the digestive organs such as the small intestine.

Vegetable fats usable in the present invention include soybean oil, cotton oil, peanut oil, flax oil, sunflower seed oil, canola oil, citron seed oil, grape seed oil, or palm oil, and preferably palm oil.

In the present invention, by coating the pulverized nanopowder with vegetable fat, yucca extract, heavy chain fatty acid and essential oil, etc., having strong antibacterial activity and carcass rate enhancing effect, pass through the stomach of the animal and show its effect in the small intestine. It becomes possible.

In the antimicrobial activity test of the antimicrobial composition for animal feed added according to the present invention prepared by the above method, it was confirmed that it exhibits strong antimicrobial activity against pathogenic bacteria when compared to commercially available antibiotics added to animal feed. This potent antimicrobial activity of the composition according to the present invention suppresses various bacterial and metabolic diseases to improve the supply and availability of nutrients and energy supplied to livestock, thereby improving the productivity of the livestock industry.

In the present invention, one or more components selected from the group consisting of yeast culture, calcium carbonate, rice husk flour, wheat flour, starch, chitin or chitosan fermentation may be added as an excipient.

Antibiotic composition for livestock feed according to the present invention prepared as described above is based on 100% by weight of the total feed, for example 0.1 to 1.2% by weight in piglet feed, about 0.1% in feed of rearing, finishing pigs, breeding pigs %, 0.1 to 0.2% by weight for the calf feed, 0.1% by weight in the feed of beef cattle, lactating cows and 0.1% by weight in other livestock feed can be used.

Antimicrobial composition for livestock feed comprising a medium chain fatty acid according to the present invention is accompanied by antibacterial action and immune enhancing action to inhibit the growth of intestinal pathogenic bacteria of the livestock, promote the growth of the livestock, and the generation of odor from livestock manure There is an excellent effect of reducing.

The antimicrobial composition for livestock feed containing the medium chain fatty acid according to the present invention significantly increases the growth and feed intake of weaning piglets when used in feed, and increases the productivity of weaning piglets than the zinc oxide addition group as well as antibiotics for promoting growth. It was very effective in improving the quality of the animal, and excellent productivity could be obtained by replacing antibiotics and zinc oxide in the weaning pig section.

Hereinafter, the present invention will be described in detail with reference to Examples. However, the examples are intended to illustrate the present invention and the scope of the present invention is not limited by the following examples.

Example 1

1 kg of yucca extract, 500 g of capnic acid and 500 g of zero coli extract were put into a fine mill and ground to a fine nanopowder. Add 2 kg of palm oil to coat the nanoparticulated powder in a rotary mixer, and then add livestock feed by mixing 500 g of chitin, 1.2 kg of yeast culture, 1.2 kg of calcium carbonate, 1.6 kg of chaff, and 1.5 kg of flour. An antimicrobial composition was prepared.

Example 2

500 g of yucca extract, 250 g of caprylic acid, 250 g of capric acid, and 500 g of zero coli extract were put into a fine grinding machine and ground to a fine powder. 2 kg of palm oil was added and the nanoparticle powder was coated in a spray mixer, followed by mixing 500 g of chitin, 1.5 kg of yeast culture, 1.5 kg of calcium carbonate, 2.0 kg of chaff, and 2.0 kg of flour. An antimicrobial composition for addition was prepared.

Test Example 1 Antimicrobial Test

Medium inoculated with Escherichia coli K88ab (Green Cross), Salmonella typhimurium (PHLS) and Staphylococcus hyicus isolated outdoors. The drugs described in Tables 1 to 3 and the antimicrobial composition according to Example 1 were each diluted in sterile distilled water to prepare a 10% solution. The drug NC, 100 times, 200 times, 400 times, 800 times, 1600 times, 3200 times, 6400 times was prepared by diluting with each culture medium, and the volume of the culture medium was 5 ml. The diluted drug was inoculated with 50 ul each of the bacterial solution cultured the previous day. After 24 hours of incubation at 37 ℃ confirmed the maximum concentration of the proliferation was not confirmed, the results are shown in Tables 1 to 3 below.

Antimicrobial Effect Test on E. coli K88ab Medication 1 Medication 2 Medication 3 Pharmaceutical 4 Pharmaceutical 5 Example 1
(5,000 ppm) NC + + + + + + 100 times - - - + + - 200 times - - - + + - 400 times - + - + + - 800 times + + - + + + 1600x + + - + + + 3200 times + + + + + + 6400 times + + + + + + result 800 times
(200ppm) 200 times
(250 ppm) 1600x
(Approximately 53 ppm) no effect no effect 400 times
(12.5 ppm)

Pharmaceutical 1: thialine (80,000 ppm)

Pharmaceutical 2: Tyrosine Phosphate (50,000 ppm)

Pharmaceutical 3: apramycin sulfate aqueous acid (85,000 ppm)

Pharmaceutical 4: Enracin-10 (1,000 ppm)

Pharmaceutical 5: BMD 10% (10,000 ppm)

Antimicrobial Effect of Salmonella typhimurium (PHLS) Medication 1 Medication 2 Pharmaceutical 3 Pharmaceutical 4 Pharmaceutical 5 Example 1
(5,000 ppm) NC + + + + + + 100 times - + - + + - 200 times - + - + + - 400 times + + - + + + 800 times + + - + + + 1600x + + + + + + 3200 times + + + + + + 6400 times + + + + + + result 200 times
(400 ppm) no effect 800 times
(About 106ppm) no effect no effect 200 times
(25 ppm)

Agents 1 to 5: see Table 1

Antimicrobial Effect Test against Staphylococcus hyicus Medication 1 Medication 2 Pharmaceutical 3 Pharmaceutical 4 Pharmaceutical 5 Example 1
(5,000 ppm) NC + + + + + + 100 times + + - + - - 200 times + + - + - - 400 times + + - + + + 800 times + + - + + + 1600x + + + + + + 3200 times + + + + + + 6400 times + + + + + + result no effect no effect 800 times
(About 106ppm) no effect 200 times
(50 ppm) 200 times
(25 ppm)

Agents 1 to 5: see Table 1

Test Example 2 Zinc Oxide Replacement Effect

A total of 600 weaned piglets were disclosed and tested in 5 treatments and 24 repetitions. The test pigs whose reasons (average 28 days old) were completed were placed by pigs in consideration of weight and sex, and the size of pigs was between 16 and 18 heads. The general composition of the feed was the same in all treatments, except for the following treatments; Control (antibiotic and zinc oxide free), AGP (44 ppm thiamoline, 100 ppm Virginiamycin), Zn25 (2,500 ppm zinc oxide), Zn15 (1,500 ppm zinc oxide) and Example 1 0.1 % Added.

Each feed was fed for 28 days after weaning, and measured and recorded the weight and feed intake of all test pigs every week from the start of the test, the results are shown in Figures 1 and 2 below.

1 and 2 show that the test pigs fed the feed added with the composition of Example 1 and the test pigs fed with the control and other drugs showed no significant difference in weight and feed intake.

Test Example 3 Carcass rate improvement effect of beef cattle

Thirty (30) castrated Korean cattle were postponed, and 15 rats were placed in the control and treatment groups. The composition of Example 1 was fed to the blended feed by adding 0.1% by weight to 100% by weight of the whole feed from 100 days before shipment.

After shipment, the conductor ratio was measured, and the results are shown in FIG. 3.

As shown in Figure 3, the treatment group to which the composition of Example 1 was added significantly increased the conductor ratio compared to the control.

The present invention is not limited to the technical spirit of the specific examples, test examples, drawings or tables described above, and the general knowledge in the technical field to which the invention belongs without departing from the spirit of the invention claimed in the claims. Of course, any person having a variety of modifications can be made, and such changes are within the scope of the claims.

1 is a graph comparing the growth rate of piglets raised with feed added to the antimicrobial composition for livestock feed according to the present invention and feed added with commercial antibiotics.

Figure 2 is a graph comparing the feed intake of piglets raised with feed added to the antimicrobial composition for livestock feed according to the invention and commercially added antibiotics.

Figure 3 is a graph comparing the carcass rate of the beef cattle reared in the feed and the general feed for the antimicrobial composition for livestock feed addition according to the present invention.

Claims (4)

5-10 wt% of Yucca extract, 5-10 wt% of medium chain fatty acid, 5-10 wt% of essential oil, 20-25 wt% of vegetable fat, 5-10 wt% of chitin or chitosan fermentation based on 100 wt% of the total composition , 12 to 15% by weight of yeast culture, 12 to 15% by weight of calcium carbonate, 16 to 20% by weight of chaff powder and 15 to 20% by weight of flour, antimicrobial composition for livestock feed addition. The antimicrobial composition for animal feed according to claim 1, wherein the medium chain fatty acid is at least one component selected from the group consisting of capronic acid, caprylic acid and capric acid. The antimicrobial composition for animal feed according to claim 1 or 2, wherein the essential oil is a zero coli extract. 1) preparing a yucca extract powder whose final moisture content is adjusted to a level of 8% or less; 2) 5 to 10% by weight of the yucca extract powder obtained in step 1), 5 to 10% by weight of at least one medium chain fatty acid selected from the group consisting of capric acid, caprylic acid and capric acid, based on 100% by weight of the total antimicrobial composition. And 5 to 10% by weight of the essential oils by mixing the fine powder into the nanopowder; 3) coating the nanopowder by adding 20 to 25 wt% of vegetable fat to 100 wt% of the total antimicrobial composition to the nanopowder obtained in step 2); And 4) 5 to 10% by weight of chitin or chitosan fermentation, 12 to 15% by weight of yeast culture, 12 to 15% by weight of calcium carbonate, based on 100% by weight of the total antimicrobial composition to the nanopowder coated with the vegetable fat obtained in step 3). , 16 to 20% by weight of chaff powder and 15 to 20% by weight of flour comprising the steps of producing an antimicrobial composition for animal feed.

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