CN104380105B - 生物样品中的分析物的检测和/或定量方法 - Google Patents

生物样品中的分析物的检测和/或定量方法 Download PDF

Info

Publication number
CN104380105B
CN104380105B CN201280065543.0A CN201280065543A CN104380105B CN 104380105 B CN104380105 B CN 104380105B CN 201280065543 A CN201280065543 A CN 201280065543A CN 104380105 B CN104380105 B CN 104380105B
Authority
CN
China
Prior art keywords
aptamer
analyte
sers
sam
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201280065543.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN104380105A (zh
Inventor
J·M·布莱克本
M·埃文斯
S·克里斯南
C·L·布罗索
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Cape Town
Original Assignee
University of Cape Town
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Cape Town filed Critical University of Cape Town
Publication of CN104380105A publication Critical patent/CN104380105A/zh
Application granted granted Critical
Publication of CN104380105B publication Critical patent/CN104380105B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56905—Protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/5695—Mycobacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2610/00—Assays involving self-assembled monolayers [SAMs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Inorganic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201280065543.0A 2011-11-02 2012-11-02 生物样品中的分析物的检测和/或定量方法 Expired - Fee Related CN104380105B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
ZA201108016 2011-11-02
ZA2011/08016 2011-11-02
PCT/IB2012/056108 WO2013065016A1 (en) 2011-11-02 2012-11-02 A method of detecting and/or quantifying an analyte in a biological sample

Publications (2)

Publication Number Publication Date
CN104380105A CN104380105A (zh) 2015-02-25
CN104380105B true CN104380105B (zh) 2017-04-12

Family

ID=47222168

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280065543.0A Expired - Fee Related CN104380105B (zh) 2011-11-02 2012-11-02 生物样品中的分析物的检测和/或定量方法

Country Status (8)

Country Link
US (1) US9518986B2 (https=)
EP (1) EP2773958B1 (https=)
CN (1) CN104380105B (https=)
BR (1) BR112014010696A2 (https=)
DK (1) DK2773958T3 (https=)
IN (1) IN2014KN01168A (https=)
WO (1) WO2013065016A1 (https=)
ZA (1) ZA201403386B (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI693403B (zh) 2019-01-17 2020-05-11 逢甲大學 免疫檢測方法

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10422792B2 (en) * 2014-03-19 2019-09-24 The Regents Of The University Of Colorado Binding detection using liquid crystal
CA2976963A1 (en) * 2015-02-19 2016-08-25 Ionica Sciences Reagents and methods for detecting infectious diseases
CN105510411B (zh) * 2015-09-14 2018-05-08 陕西师范大学 基于细胞与微电极的相互作用实现单一癌细胞的检测方法
DE102015220414A1 (de) * 2015-10-20 2017-04-20 Robert Bosch Gmbh Propofolsensor zur Messung einer Narkosetiefe
CN105277527A (zh) * 2015-10-29 2016-01-27 江南大学 一种基于拉曼信标分子编码银@金核壳纳米粒子的真菌毒素双重检测方法
KR101816521B1 (ko) * 2016-01-28 2018-01-09 광주과학기술원 삽입제를 이용한 국소표면플라즈몬공명 기반 고감도 압타머 센서
US10103357B2 (en) 2016-02-17 2018-10-16 The Curators Of The University Of Missouri Fabrication of multilayer nanograting structures
CN109791546A (zh) * 2016-10-28 2019-05-21 惠普发展公司有限责任合伙企业 目标类别特征模型
KR101923196B1 (ko) * 2017-09-25 2018-11-28 주식회사 엠디엡투스 Cfp10에 특이적으로 결합하는 dna 압타머 및 이의 용도
WO2019126182A1 (en) * 2017-12-18 2019-06-27 The University Of Memphis Research Foundation Compositions and methods for the detection and molecular profiling of membrane bound vesicles
TWI686599B (zh) * 2018-05-24 2020-03-01 國立清華大學 塵蟎抗原檢測系統及方法
MY204234A (en) 2018-08-27 2024-08-16 Regeneron Pharma Use of raman spectroscopy in downstream purification
WO2020100159A1 (en) * 2018-11-13 2020-05-22 Translational Health Science And Technology Institute A novel aptamer and an electrochemical biosensor for the rapid detection and diagnosis of tuberculous meningitis
WO2020132074A1 (en) 2018-12-18 2020-06-25 The University Of Memphis Research Foundation Compositions and methods for the detection and molecular profiling of membrane bound vesicles
EP3953686A4 (en) * 2019-04-09 2022-12-21 Agency for Science, Technology and Research Label-free detection of mycobacteria using surface enhanced raman spectroscopy
CN110907643A (zh) * 2019-12-02 2020-03-24 中国科学院重庆绿色智能技术研究院 一种大肠杆菌检测芯片的制备方法及检测芯片
CN115836131A (zh) * 2020-05-29 2023-03-21 索莫纳斯科技有限公司 一种丙泊酚传感器
TWI759776B (zh) * 2020-06-22 2022-04-01 淡江大學 檢測基板、拉曼光譜檢測系統及拉曼光譜檢測方法
TW202225689A (zh) * 2020-09-24 2022-07-01 美商創科精密醫學公司 用於偵測病原體之系統、裝置及方法
CN112240881A (zh) * 2020-09-30 2021-01-19 合肥国研汉因检测科技有限公司 使用拉曼光谱法快速检测痕量芬太尼的方法
CN112986212B (zh) * 2021-03-10 2023-01-17 中国人民解放军海军军医大学 一种g-四链体核酸适配体与小分子相互作用的研究方法
CN114577754A (zh) * 2021-12-24 2022-06-03 天津大学 基于适体变构效应的微型光纤表面等离子共振传感器
CN115711873B (zh) * 2022-11-09 2024-05-28 清华大学 一种利用sers文件卡片进行定量分析的方法
CN117054389A (zh) * 2023-07-13 2023-11-14 淮安市第五人民医院 一种用于快速鉴定胃部幽门螺旋杆菌感染的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1938430A (zh) * 2003-12-30 2007-03-28 英特尔公司 使用拉曼活性探针构建物来分析生物学样品的方法和装置
US20100105053A1 (en) * 2008-10-10 2010-04-29 Hansang Cho Aptamer based sensors and related methods and systems

Family Cites Families (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5376556A (en) 1989-10-27 1994-12-27 Abbott Laboratories Surface-enhanced Raman spectroscopy immunoassay
EP0786469B1 (en) 1990-06-11 2006-03-01 Gilead Sciences, Inc. Methods of use of nucleic acid ligands
EP0598003B1 (en) 1991-07-22 1999-02-10 Kloehn Instruments, Ltd. Substrate for surface-enhanced analytical procedures, and substrates prepared for specific procedures
JP2965502B2 (ja) 1996-02-20 1999-10-18 株式会社京都第一科学 表面増強ラマン散乱測定による分析方法
GB9810865D0 (en) 1998-05-20 1998-07-22 Zeneca Ltd Nucleic acid sequence identification
ATE419528T1 (de) 1999-04-28 2009-01-15 Eidgenoess Tech Hochschule Polyionische beschichtungen für analytische und sensor-vorrichtungen
PT1226422E (pt) 1999-10-06 2008-03-04 Oxonica Inc Nanopartículas compósitas activas em espectroscopia intensificada por superfície
DE10050632A1 (de) 2000-10-12 2002-04-18 Stiftung Caesar Verfahren zum Nachweis biologischer Moleküle
US7238477B2 (en) 2001-09-24 2007-07-03 Intel Corporation Methods to increase nucleotide signals by Raman scattering
US7807348B2 (en) 2002-03-20 2010-10-05 Wisconsin Alumni Research Foundation Optical imaging of nanostructured substrates
US7361313B2 (en) 2003-02-18 2008-04-22 Intel Corporation Methods for uniform metal impregnation into a nanoporous material
US7192703B2 (en) 2003-02-14 2007-03-20 Intel Corporation, Inc. Biomolecule analysis by rolling circle amplification and SERS detection
US7351591B2 (en) 2004-03-30 2008-04-01 Intel Corporation Surface modification of metals for biomolecule detection using surface enhanced Raman scattering (SERS)
DK1749122T3 (da) 2004-04-23 2019-11-11 Becton Dickinson Co Overfladeforbedrede spektroskopi-aktive kompositnanopartikler
CN101014729A (zh) 2004-06-07 2007-08-08 格莱风分析有限责任公司 制造用于sers的基底表面的系统和方法以及使用上述系统和方法的设备
CA2586197C (en) 2004-11-04 2012-08-14 Mesophotonics Limited Metal nano-void photonic crystal for enhanced raman spectroscopy
US7485471B1 (en) 2004-12-17 2009-02-03 Intel Corporation Detection of enhanced multiplex signals by surface enhanced Raman spectroscopy
US7355704B2 (en) 2005-06-13 2008-04-08 Solaris Nanosciences, Inc. Chemical and biological sensing using metallic particles in amplifying and absorbing media
US20070134815A1 (en) 2005-07-11 2007-06-14 Danielle Chamberlin Sensitivity enhancement of POCT devices using gold and silver nanoparticles on substrates containing nanostructures or nanoparticles that interact with labeling particles
US7651863B2 (en) 2005-07-14 2010-01-26 3M Innovative Properties Company Surface-enhanced spectroscopic method, flexible structured substrate, and method of making the same
JP2009523406A (ja) 2005-11-15 2009-06-25 オクソニカ・インコーポレーテッド 生体剤（ｂｉｏａｇｅｎｔｓ）の検出のためのＳＥＲＳに基づく方法
GB0524580D0 (en) * 2005-12-01 2006-01-11 Sphere Medical Ltd Sensor
GB0606088D0 (en) 2006-03-27 2006-05-03 E2V Biosensors Ltd Improved serrs substrate
ES2635272T3 (es) 2007-03-20 2017-10-03 Becton, Dickinson And Company Ensayo que usa partículas activas en espectroscopia Raman potenciada en superficie (SERS)
US8017408B2 (en) 2007-04-27 2011-09-13 The Regents Of The University Of California Device and methods of detection of airborne agents
KR100892629B1 (ko) 2007-06-29 2009-04-08 한국과학기술원 표면 증강 라만 분광용 광 센서
JP2009031023A (ja) 2007-07-25 2009-02-12 Keio Gijuku 表面増強ラマン分光分析用基板の作成方法、マイクロｔａｓの製造方法、及び、マイクロｔａｓ
US20100055803A1 (en) 2008-08-29 2010-03-04 Kwangyeol Lee Method and apparatus for detecting molecules
US8947657B2 (en) * 2008-09-08 2015-02-03 Lawrence Livermore National Security, Llc Methods for isolation and viability assessment of biological organisms
CA2783788C (en) * 2009-12-11 2015-02-10 Korea Research Institute Of Chemical Technology Dimeric core-shell nanostructure labeled with raman active molecule localized at interparticle junction, use thereof, and method for preparing the same
TWI487910B (zh) * 2010-09-07 2015-06-11 國立成功大學 倒多角錐陣列型免標定病毒檢測基板、系統及方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1938430A (zh) * 2003-12-30 2007-03-28 英特尔公司 使用拉曼活性探针构建物来分析生物学样品的方法和装置
US20100105053A1 (en) * 2008-10-10 2010-04-29 Hansang Cho Aptamer based sensors and related methods and systems

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
A disposable microfluidic biochip with on-chip molecularly imprinted biosensors for optical detection of anesthetic propofol;Chien-Chong Hong;《Biosensors and Bioelectronics》;20100210;第25卷;第2058-2064页 *
Antibody-functionalized SERS tags with improved sensitivity;Nekane Guarrotxena;《Chem.Commun.》;20110707;第47卷;图1，第8784页右栏第2段 *
Aptamer-Based SERRS Sensor for Thrombin Detection;Hansang Cho;《nano letters》;20080411;第8卷(第12期);第4386-4390页 *
Characterisation and identification of bacteria using SERS;Roger M.Jarvis;《Chemical Society Reviews》;20080531;第37卷;第931-936页 *
Direct Optical Detection of Aptamer Conformational Changes Induced by Target Molecules;Oara Neumann;《Anal.Chem.》;20091215;第81卷;第10002-10006页 *
Fiber optic SPR biosensing of DNA hybridization and DNA–protein interactions;Jeroen Pollet;《Biosensors and Bioelectronics》;20090903;第25卷;第864-869页 *
Recent progress in SERS biosensing;Haynes;《Physical Chemistry Chemical Physics》;20110628;第13卷(第24期);第11453-11776页 *
Surface immobilization of DNA aptamers for biosensing and protein interaction analysis;XiaojuanZhang;《Biosensors and Bioelectronics》;20101216;第26卷;摘要，图1，第3143页右栏第2-3段 *
基于核酸适配体和纳米粒子的光学探针;王国庆;《化学进展》;20100331;第22卷;第489-599页 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI693403B (zh) 2019-01-17 2020-05-11 逢甲大學 免疫檢測方法

Also Published As

Publication number Publication date
US20140302492A1 (en) 2014-10-09
EP2773958B1 (en) 2017-11-29
IN2014KN01168A (https=) 2015-10-16
ZA201403386B (en) 2016-02-24
BR112014010696A2 (pt) 2017-04-25
EP2773958A1 (en) 2014-09-10
US9518986B2 (en) 2016-12-13
WO2013065016A1 (en) 2013-05-10
CN104380105A (zh) 2015-02-25
DK2773958T3 (en) 2018-03-12

Similar Documents

Publication Publication Date Title
CN104380105B (zh) 生物样品中的分析物的检测和/或定量方法
Raveendran et al. Rational design of DNA nanostructures for single molecule biosensing
Dey et al. Single-molecule optical biosensing: recent advances and future challenges
Jain The role of nanobiotechnology in drug discovery
Hassen et al. An impedimetric DNA sensor based on functionalized magnetic nanoparticles for HIV and HBV detection
JP2022058772A (ja) 試料分析のためのデバイスおよび方法
Xu et al. Label-free electrochemical detection for aptamer-based array electrodes
Liu et al. Aptamer-based electrochemical biosensor for interferon gamma detection
Marotta et al. Limitations of surface enhanced Raman scattering in sensing DNA hybridization demonstrated by label-free DNA oligos as molecular rulers of distance-dependent enhancement
Niedzwiecki et al. Sampling a biomarker of the human immunodeficiency virus across a synthetic nanopore
Zhang et al. SERS nanotags and their applications in biosensing and bioimaging
US20240076715A1 (en) Force-controlled nanoswitch assays for single-molecule detection in complex biological fluids
Zhu et al. Frequency shift surface-enhanced Raman spectroscopy sensing: An ultrasensitive multiplex assay for biomarkers in human health
JP2009540326A (ja) 結合及び非結合ラベルの測定による検体検出の増加した特異性
Liao et al. Hybridization chain reaction triggered poly adenine to absorb silver nanoparticles for label-free electrochemical detection of Alzheimer's disease biomarkers amyloid β-peptide oligomers
WO2012044387A2 (en) Methods and compositions for detection of analytes
Huo et al. ATP-responsive strand displacement coupling with DNA origami/AuNPs strategy for the determination of microcystin-LR using surface-enhanced Raman spectroscopy
Deng et al. A CRISPR/Cas12a-assisted on-fibre immunosensor for ultrasensitive small protein detection in complex biological samples
Wang et al. Evaluation of medicine effects on the interaction of myoglobin and its aptamer or antibody using atomic force microscopy
Wang et al. Microswimmer-based electrochemical platform with active capturer/signal amplifier/funnel-type device for whole blood detection
Sang et al. Real-time and label-free detection of VKORC1 genes based on a magnetoelastic biosensor for warfarin therapy
Michelle Grandin et al. Bioinspired, nanoscale approaches in contemporary bioanalytics
Wang et al. Dual-Functionalized Glass Micropipette Sensor for Simultaneous High Sensitivity Detection of Cancer Biomarkers
Hinman et al. Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing
Singla et al. Early detection of SARS-CoV-2 with functionalized gold and molecularly imprinted polymeric nanoparticles: a mini review

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20170412

Termination date: 20211102

CF01 Termination of patent right due to non-payment of annual fee