CN104379760B - 用于流式细胞术的样品制备 - Google Patents
用于流式细胞术的样品制备 Download PDFInfo
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- CN104379760B CN104379760B CN201380029083.0A CN201380029083A CN104379760B CN 104379760 B CN104379760 B CN 104379760B CN 201380029083 A CN201380029083 A CN 201380029083A CN 104379760 B CN104379760 B CN 104379760B
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- YIWGJFPJRAEKMK-UHFFFAOYSA-N 1-(2H-benzotriazol-5-yl)-3-methyl-8-[2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carbonyl]-1,3,8-triazaspiro[4.5]decane-2,4-dione Chemical compound CN1C(=O)N(c2ccc3n[nH]nc3c2)C2(CCN(CC2)C(=O)c2cnc(NCc3cccc(OC(F)(F)F)c3)nc2)C1=O YIWGJFPJRAEKMK-UHFFFAOYSA-N 0.000 description 3
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- CVOFKRWYWCSDMA-UHFFFAOYSA-N 2-chloro-n-(2,6-diethylphenyl)-n-(methoxymethyl)acetamide;2,6-dinitro-n,n-dipropyl-4-(trifluoromethyl)aniline Chemical compound CCC1=CC=CC(CC)=C1N(COC)C(=O)CCl.CCCN(CCC)C1=C([N+]([O-])=O)C=C(C(F)(F)F)C=C1[N+]([O-])=O CVOFKRWYWCSDMA-UHFFFAOYSA-N 0.000 description 1
- XVMSFILGAMDHEY-UHFFFAOYSA-N 6-(4-aminophenyl)sulfonylpyridin-3-amine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)C1=CC=C(N)C=N1 XVMSFILGAMDHEY-UHFFFAOYSA-N 0.000 description 1
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical compound C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 1
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- 101100412856 Mus musculus Rhod gene Proteins 0.000 description 1
- VCUFZILGIRCDQQ-KRWDZBQOSA-N N-[[(5S)-2-oxo-3-(2-oxo-3H-1,3-benzoxazol-6-yl)-1,3-oxazolidin-5-yl]methyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C1O[C@H](CN1C1=CC2=C(NC(O2)=O)C=C1)CNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F VCUFZILGIRCDQQ-KRWDZBQOSA-N 0.000 description 1
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- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical group OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/06—Quantitative determination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Plural Heterocyclic Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261620823P | 2012-04-05 | 2012-04-05 | |
| US61/620,823 | 2012-04-05 | ||
| PCT/US2013/035291 WO2013152203A1 (en) | 2012-04-05 | 2013-04-04 | Sample preparation for flow cytometry |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104379760A CN104379760A (zh) | 2015-02-25 |
| CN104379760B true CN104379760B (zh) | 2018-03-16 |
Family
ID=49301050
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201380029083.0A Active CN104379760B (zh) | 2012-04-05 | 2013-04-04 | 用于流式细胞术的样品制备 |
Country Status (7)
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11001881B2 (en) | 2006-08-24 | 2021-05-11 | California Institute Of Technology | Methods for detecting analytes |
| US8048626B2 (en) | 2006-07-28 | 2011-11-01 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11525156B2 (en) | 2006-07-28 | 2022-12-13 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US11560588B2 (en) | 2006-08-24 | 2023-01-24 | California Institute Of Technology | Multiplex Q-PCR arrays |
| US9933341B2 (en) | 2012-04-05 | 2018-04-03 | Becton, Dickinson And Company | Sample preparation for flow cytometry |
| CA2905651C (en) * | 2013-03-13 | 2019-01-15 | Becton, Dickinson And Company | A method for improving analysis of microorganisms in complex matrices |
| CN104677809B (zh) * | 2013-11-29 | 2018-01-02 | 中国人民解放军第二军医大学 | 隐球菌活力检测试剂盒及检测方法 |
| US9708647B2 (en) | 2015-03-23 | 2017-07-18 | Insilixa, Inc. | Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays |
| US9499861B1 (en) | 2015-09-10 | 2016-11-22 | Insilixa, Inc. | Methods and systems for multiplex quantitative nucleic acid amplification |
| WO2017155858A1 (en) | 2016-03-07 | 2017-09-14 | Insilixa, Inc. | Nucleic acid sequence identification using solid-phase cyclic single base extension |
| CN107643238A (zh) * | 2017-09-19 | 2018-01-30 | 中山大学附属第医院 | 一种定量检测血液循环微粒浓度的方法 |
| US12196652B2 (en) * | 2018-08-01 | 2025-01-14 | Sartorius Bioanalytical Instruments, Inc. | Methods, kits and stain compositions for flow cytometry evaluation of unassociated virus-size particles using multiple fluorogenic dyes |
| CN119510370A (zh) | 2019-03-14 | 2025-02-25 | 因斯利克萨公司 | 基于时间门控的荧光检测的方法和系统 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6323337B1 (en) * | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
| US7205100B2 (en) * | 2002-10-10 | 2007-04-17 | Advanced Analytical Technologies, Inc. | Methods for reducing background fluorescence |
| WO2011160887A1 (en) * | 2010-06-21 | 2011-12-29 | Siemens Healthcare Diagnostics Inc. | Soluble quencher to reduce background in qpcr assays |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5436134A (en) * | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| JP3215428B2 (ja) * | 1995-01-16 | 2001-10-09 | ソイニ，エルッキ | 生物特異性マルチパラメーターアッセイ法 |
| US20030013849A1 (en) * | 1999-10-29 | 2003-01-16 | Ward William W. | Renilla reniformis green fluorescent protein |
| RU2172512C1 (ru) * | 2000-02-01 | 2001-08-20 | Закрытое акционерное общество Научно-производственное объединение "ФоМос" | Цветной спектрозональный галогенсеребряный фотографический материал |
| WO2002042267A2 (en) * | 2000-11-17 | 2002-05-30 | Biomoda, Inc. | Compositions and methods for detecting pre-cancerous conditions in cell and tissue samples using 5, 10, 15, 20-tetrakis (carboxyphenyl) porphine |
| US8481302B2 (en) * | 2008-11-03 | 2013-07-09 | General Electric Company | Total bacteria monitoring system |
| GB201005939D0 (en) * | 2010-04-09 | 2010-05-26 | Biostatus Ltd | Method of analysing a cell or other biological material containing nucleic acid |
-
2013
- 2013-04-04 CA CA2869472A patent/CA2869472C/en active Active
- 2013-04-04 CN CN201380029083.0A patent/CN104379760B/zh active Active
- 2013-04-04 IN IN8957DEN2014 patent/IN2014DN08957A/en unknown
- 2013-04-04 WO PCT/US2013/035291 patent/WO2013152203A1/en active Application Filing
- 2013-04-04 EP EP13773119.6A patent/EP2834367A4/en not_active Withdrawn
- 2013-04-04 JP JP2015504727A patent/JP6401146B2/ja active Active
- 2013-04-04 AU AU2013243378A patent/AU2013243378B2/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6323337B1 (en) * | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
| US7205100B2 (en) * | 2002-10-10 | 2007-04-17 | Advanced Analytical Technologies, Inc. | Methods for reducing background fluorescence |
| WO2011160887A1 (en) * | 2010-06-21 | 2011-12-29 | Siemens Healthcare Diagnostics Inc. | Soluble quencher to reduce background in qpcr assays |
Non-Patent Citations (2)
| Title |
|---|
| 3.3′二乙基硫醛三碳菁化碘拉曼光谱研究;蔡红星等;《光谱学与光谱分析》;20101231;第30卷(第12期);3244-3248 * |
| 流式细胞计在免疫荧光测量中去除死细胞影响的方法;陶家平等;《生物化学与生物物理进展》;19901231;第17卷(第1期);298-301 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013152203A1 (en) | 2013-10-10 |
| EP2834367A4 (en) | 2016-03-23 |
| CA2869472A1 (en) | 2013-10-10 |
| AU2013243378B2 (en) | 2019-04-11 |
| EP2834367A1 (en) | 2015-02-11 |
| AU2013243378A1 (en) | 2014-10-30 |
| JP6401146B2 (ja) | 2018-10-03 |
| IN2014DN08957A (enrdf_load_stackoverflow) | 2015-05-22 |
| CN104379760A (zh) | 2015-02-25 |
| JP2015514403A (ja) | 2015-05-21 |
| CA2869472C (en) | 2017-07-11 |
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