AU2013243378B2 - Sample preparation for flow cytometry - Google Patents

Sample preparation for flow cytometry Download PDF

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Publication number
AU2013243378B2
AU2013243378B2 AU2013243378A AU2013243378A AU2013243378B2 AU 2013243378 B2 AU2013243378 B2 AU 2013243378B2 AU 2013243378 A AU2013243378 A AU 2013243378A AU 2013243378 A AU2013243378 A AU 2013243378A AU 2013243378 B2 AU2013243378 B2 AU 2013243378B2
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AU
Australia
Prior art keywords
sample
nucleic acid
moiety
background signal
acid stain
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Active
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AU2013243378A
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English (en)
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AU2013243378A1 (en
Inventor
Kenneth Anthony Kopher
Xiao Li
John D. Mantlo
William Alfred Pope
Song Shi
Axel A. Yup
Yongqiang Zhang
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Becton Dickinson and Co
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Becton Dickinson and Co
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Publication of AU2013243378A1 publication Critical patent/AU2013243378A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Plural Heterocyclic Compounds (AREA)
AU2013243378A 2012-04-05 2013-04-04 Sample preparation for flow cytometry Active AU2013243378B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261620823P 2012-04-05 2012-04-05
US61/620,823 2012-04-05
PCT/US2013/035291 WO2013152203A1 (en) 2012-04-05 2013-04-04 Sample preparation for flow cytometry

Publications (2)

Publication Number Publication Date
AU2013243378A1 AU2013243378A1 (en) 2014-10-30
AU2013243378B2 true AU2013243378B2 (en) 2019-04-11

Family

ID=49301050

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013243378A Active AU2013243378B2 (en) 2012-04-05 2013-04-04 Sample preparation for flow cytometry

Country Status (7)

Country Link
EP (1) EP2834367A4 (enrdf_load_stackoverflow)
JP (1) JP6401146B2 (enrdf_load_stackoverflow)
CN (1) CN104379760B (enrdf_load_stackoverflow)
AU (1) AU2013243378B2 (enrdf_load_stackoverflow)
CA (1) CA2869472C (enrdf_load_stackoverflow)
IN (1) IN2014DN08957A (enrdf_load_stackoverflow)
WO (1) WO2013152203A1 (enrdf_load_stackoverflow)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9933341B2 (en) 2012-04-05 2018-04-03 Becton, Dickinson And Company Sample preparation for flow cytometry
CA2905651C (en) * 2013-03-13 2019-01-15 Becton, Dickinson And Company A method for improving analysis of microorganisms in complex matrices
CN104677809B (zh) * 2013-11-29 2018-01-02 中国人民解放军第二军医大学 隐球菌活力检测试剂盒及检测方法
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
CN107643238A (zh) * 2017-09-19 2018-01-30 中山大学附属第医院 一种定量检测血液循环微粒浓度的方法
US12196652B2 (en) * 2018-08-01 2025-01-14 Sartorius Bioanalytical Instruments, Inc. Methods, kits and stain compositions for flow cytometry evaluation of unassociated virus-size particles using multiple fluorogenic dyes
CN119510370A (zh) 2019-03-14 2025-02-25 因斯利克萨公司 基于时间门控的荧光检测的方法和系统

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method
EP1193547A1 (en) * 2000-02-01 2002-04-03 Zakrytoe Aktsionernoe Obschestvo Ordena Trudovogo Krasnogo Znameni Nauchno-Proizvodstvennoe Obiedinenie "Fomos" (ZAO NPO... Colored spectrozonal silver-halide photographic material

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US20030013849A1 (en) * 1999-10-29 2003-01-16 Ward William W. Renilla reniformis green fluorescent protein
US6323337B1 (en) * 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
WO2002042267A2 (en) * 2000-11-17 2002-05-30 Biomoda, Inc. Compositions and methods for detecting pre-cancerous conditions in cell and tissue samples using 5, 10, 15, 20-tetrakis (carboxyphenyl) porphine
US7205100B2 (en) * 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
WO2011160887A1 (en) * 2010-06-21 2011-12-29 Siemens Healthcare Diagnostics Inc. Soluble quencher to reduce background in qpcr assays

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method
EP1193547A1 (en) * 2000-02-01 2002-04-03 Zakrytoe Aktsionernoe Obschestvo Ordena Trudovogo Krasnogo Znameni Nauchno-Proizvodstvennoe Obiedinenie "Fomos" (ZAO NPO... Colored spectrozonal silver-halide photographic material

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Hofer, L. J. E. et al, "The fluorescence of cyanine and related dyes in the monomeric state", Journal of the American Chemical Society, 1950, Vol. 72, pp 203-209 *
JUNG KYU CHOI ET AL, "3,3'-Diethylthiatricarbocyanine Iodide: A Highly Sensitive Chiroptical Reporter of DNA Helicity and Sequence", INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, (2011-11-16), vol. 12, no. 12, pages 8052 - 8062 *

Also Published As

Publication number Publication date
WO2013152203A1 (en) 2013-10-10
EP2834367A4 (en) 2016-03-23
CN104379760B (zh) 2018-03-16
CA2869472A1 (en) 2013-10-10
EP2834367A1 (en) 2015-02-11
AU2013243378A1 (en) 2014-10-30
JP6401146B2 (ja) 2018-10-03
IN2014DN08957A (enrdf_load_stackoverflow) 2015-05-22
CN104379760A (zh) 2015-02-25
JP2015514403A (ja) 2015-05-21
CA2869472C (en) 2017-07-11

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