IN2014DN08957A - - Google Patents

Info

Publication number
IN2014DN08957A
IN2014DN08957A IN8957DEN2014A IN2014DN08957A IN 2014DN08957 A IN2014DN08957 A IN 2014DN08957A IN 8957DEN2014 A IN8957DEN2014 A IN 8957DEN2014A IN 2014DN08957 A IN2014DN08957 A IN 2014DN08957A
Authority
IN
India
Prior art keywords
sample
analyzing
nucleic acid
background signal
acid stain
Prior art date
Application number
Inventor
Xiao Li
Yongqiang Zhang
Kenneth Anthony Kopher
John D Mantlo
William Alfred Pope
Song Shi
Axel A Yup
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Publication of IN2014DN08957A publication Critical patent/IN2014DN08957A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Plural Heterocyclic Compounds (AREA)

Abstract

Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain.
IN8957DEN2014 2012-04-05 2013-04-04 IN2014DN08957A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261620823P 2012-04-05 2012-04-05
PCT/US2013/035291 WO2013152203A1 (en) 2012-04-05 2013-04-04 Sample preparation for flow cytometry

Publications (1)

Publication Number Publication Date
IN2014DN08957A true IN2014DN08957A (en) 2015-05-22

Family

ID=49301050

Family Applications (1)

Application Number Title Priority Date Filing Date
IN8957DEN2014 IN2014DN08957A (en) 2012-04-05 2013-04-04

Country Status (7)

Country Link
EP (1) EP2834367A4 (en)
JP (1) JP6401146B2 (en)
CN (1) CN104379760B (en)
AU (1) AU2013243378B2 (en)
CA (1) CA2869472C (en)
IN (1) IN2014DN08957A (en)
WO (1) WO2013152203A1 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11001881B2 (en) 2006-08-24 2021-05-11 California Institute Of Technology Methods for detecting analytes
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US11525156B2 (en) 2006-07-28 2022-12-13 California Institute Of Technology Multiplex Q-PCR arrays
US11560588B2 (en) 2006-08-24 2023-01-24 California Institute Of Technology Multiplex Q-PCR arrays
US9645057B2 (en) 2012-04-05 2017-05-09 Becton, Dickiinson and Company Method for improving analysis of microorganisms in complex matrices
EP2971050B1 (en) * 2013-03-13 2018-12-19 Becton, Dickinson and Company A method for improving analysis of microorganisms in complex matrices
CN104677809B (en) * 2013-11-29 2018-01-02 中国人民解放军第二军医大学 Cryptococcus viability detection kit and detection method
US9708647B2 (en) 2015-03-23 2017-07-18 Insilixa, Inc. Multiplexed analysis of nucleic acid hybridization thermodynamics using integrated arrays
US9499861B1 (en) 2015-09-10 2016-11-22 Insilixa, Inc. Methods and systems for multiplex quantitative nucleic acid amplification
WO2017155858A1 (en) * 2016-03-07 2017-09-14 Insilixa, Inc. Nucleic acid sequence identification using solid-phase cyclic single base extension
CN107643238A (en) * 2017-09-19 2018-01-30 中山大学附属第医院 Method for quantitatively detecting concentration of blood circulation particles
US12196652B2 (en) * 2018-08-01 2025-01-14 Sartorius Bioanalytical Instruments, Inc. Methods, kits and stain compositions for flow cytometry evaluation of unassociated virus-size particles using multiple fluorogenic dyes
JP7687957B2 (en) 2019-03-14 2025-06-03 インシリクサ， インコーポレイテッド Methods and systems for time-gated fluorescence-based detection

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
WO1996022531A1 (en) * 1995-01-16 1996-07-25 Erkki Soini A biospecific multiparameter assay method
US20030013849A1 (en) * 1999-10-29 2003-01-16 Ward William W. Renilla reniformis green fluorescent protein
RU2172512C1 (en) * 2000-02-01 2001-08-20 Закрытое акционерное общество Научно-производственное объединение "ФоМос" Colored spectrum-zone silver halide photographic material
US6323337B1 (en) * 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
AU3926902A (en) * 2000-11-17 2002-06-03 Biomoda Inc Compositions and methods for detecting pre-cancerous conditions in cell and tissue samples using 5, 10, 15, 20-tetrakis (carboxyphenyl) porphine
US7205100B2 (en) * 2002-10-10 2007-04-17 Advanced Analytical Technologies, Inc. Methods for reducing background fluorescence
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
EP2582838B1 (en) * 2010-06-21 2014-07-16 Siemens Healthcare Diagnostics Inc. Soluble quencher to reduce background in qpcr assays

Also Published As

Publication number Publication date
JP6401146B2 (en) 2018-10-03
CA2869472C (en) 2017-07-11
AU2013243378B2 (en) 2019-04-11
CN104379760B (en) 2018-03-16
WO2013152203A1 (en) 2013-10-10
EP2834367A1 (en) 2015-02-11
JP2015514403A (en) 2015-05-21
CN104379760A (en) 2015-02-25
AU2013243378A1 (en) 2014-10-30
CA2869472A1 (en) 2013-10-10
EP2834367A4 (en) 2016-03-23

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