CN104365218B - A kind of method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout - Google Patents
A kind of method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout Download PDFInfo
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- CN104365218B CN104365218B CN201410710696.7A CN201410710696A CN104365218B CN 104365218 B CN104365218 B CN 104365218B CN 201410710696 A CN201410710696 A CN 201410710696A CN 104365218 B CN104365218 B CN 104365218B
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Abstract
The invention discloses a kind of method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout.The method is: before kafir lily planting seed, described seed is carried out immersion treatment by the ethylmethane sulfonate solution adopting concentration to be 0.15%～0.2% (v/v), after immersion, the ethylmethane sulfonate on seed is rinsed well, then sow.The method also includes: after the kafir lily planting seed after described process, carries out a point cultivation when kafir lily seedling length is to 1.2～1.8 centimetres high, carries out field planting after point cultivation when kafir lily grows to 4～5 blades.In the present invention, after the kafir lily planting seed that EMS processes, will not increasing visible variation, emergence rate is similar to untreated material, but can be effectively improved the rate in strong sprout of kafir lily.Although the seed after processing is slower at sowing primary growth, but growth will ramp up after 12 months, and show that growth potential is strong, short strong, the feature in strong sprout that well developed root system, blade plumpness are bright of plant.
Description
Technical field
The invention belongs to kafir lily technical field of plant cultivation, be specifically related to a kind of method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout.
Background technology
Ethylmethane sulfonate, English name is Ethylmethylsulfone (being abbreviated as EMS, EMS used below represents ethylmethane sulfonate), and molecular formula is CH3SO2OC2H5, it is a kind of point mutation chemical mutagen, is usually used in the initiative of new germ plasm and mutant library.Discovered in recent years, EMS, except having mutagenesis, also has plant growth regulation.The research of Zhang Bingxue, Wen Xiaopeng shows, soak seed Hylocereus undatus seed with the EMS short time of low concentration, germination speed and regularity (EMS impact on Seed Germination of Pitaya, mountain farming biology journal, 2013 (3): 194～197) can be improved.Bibliographical information is had to show: to use the concentration EMS less than 0.1% to process Crambe abyssinica seed, germinating energy and germination percentage (Wang Youping, Xu Xiaoxia, Jia Yongjiong of seed can be improved, ethylmethane sulfonate and the 60Co physiological effect to Crambe abyssinica germination seed. Plant Physiology Communications, 1998,34 (2): 101-103).By the EMS Rice seeds treated of 0.5%, can promote seminal root, bud growth (Chen Can, Xu Qingguo, Peng Bo etc., the impact on rice seed germination and growth of the different chemical mutagenic agent. seed, 2008,27 (3) 9～13).EMS with 0.1% processes tobacco seed, seed root length there is facilitation, EMS to the mutation of plant and physiological effect and reagent concentration, process time and temperature relevant (Wang Junwei, Jiang Caihong, Song Zhimei etc., ethylmethane sulfonate Treatment Effects [J] .2011 to tobacco seed germination percentage, 32 (3): 17 27).
Kafir lily (Cliviaminiata) is the perennial evergreen herbaceous plant of a kind of Amaryllidaceae gentleman Cymbidium, originates in southern South africa；The appreciation effect that it has " four seasons are seen leaf, see fruit three seasons, admire the beauty of flowers in a season ", is world-renowned indoor high-grade potted flowers, and all there is cultivation China various places.Kafir lily is mainly through seed and division propagation nursery.Seed seedling-raising breeding coefficient is high, and speed is fast, and seedling age is consistent, can meet the needs of market and large-scale production better, be the main path of kafir lily nursery.The kafir lily of China is viewed and admired with Ye Yiwei master, with external with floriculture for main different, by seminal propagation nurturing staff, can not only lay the foundation for its later stage robust growth, and can improve sight and the commodity of its vegetative growth phase.
The kafir lily rate in strong sprout of current China is generally relatively low.Owing to climate environmental factors limits, China kafir lily mainly originates in the northeast such as Jilin, Liaoning one band for a long time, but the kafir lily Seedling that the north is cultivated is poor at south adaptability, and local in high temperature, high humidity, the south that has a narrow range of temperature to cultivate kafir lily increasingly difficult for strong sprout, in nursery, ubiquity growth is not strengthened, root system is less-developed, resistance is poor, the problems such as easy excessive growth in summer, therefore find the short-cut method in a kind of applicable southern area quickly breeding strong sprout and kafir lily industry development are significant.
Summary of the invention
It is an object of the present invention to provide a kind of method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout, it is intended to solve kafir lily in seedling raising process strong sprout difficulty technical problem.
The present invention solves that the technical scheme that above-mentioned technical problem adopts is as follows:
A kind of method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout, the method is: before kafir lily planting seed, described seed is carried out immersion treatment by the ethylmethane sulfonate solution adopting concentration to be 0.15%～0.2% (v/v), after immersion, the ethylmethane sulfonate on seed is rinsed well, then sow.
Preferably, described ethylmethane sulfonate solution adopts phosphate buffer to prepare.The concentration of described phosphate buffer is 0.1mol/L, pH value is 6.8.
Preferably, seed is carried out the condition of immersion treatment and is by described ethylmethane sulfonate solution: temperature during ethylmethane sulfonate solution soaking is 26～30 DEG C, and soak time is 4～6 hours.
Preferably, when seed is soaked by described ethylmethane sulfonate solution, ethylmethane sulfonate solution is positioned on shaking table and makes seed follow solution to shake, to reach to soak uniform purpose.Specifically, described shaking speed is set to 200 revs/min.
Preferably, described method also includes: after the kafir lily planting seed after described process, carries out a point cultivation in kafir lily seedling length to period 1.2～1.8 centimetres high, carries out field planting after point cultivation when the kafir lily number of blade reaches 4～5 leaf.
Compared with prior art, beneficial effects of the present invention is as follows:
1, adopt the EMS of low concentration to process kafir lily seed under given conditions, there is the effect of regulation and control kafir lily growth, and visible variation will not be increased, emergence rate is similar to untreated material, but can be effectively improved the rate in strong sprout of kafir lily.Although EMS process after planting seed preliminary phase untreated seedling early growth is slower, but long grow and will ramp up to 4～5 blades, and show that growth potential is strong, plant short is strengthened, well developed root system, blade plumpness are bright feature in strong sprout.Being respectively greater than seedling meansigma methods of the same age as Index of The Healthy Seedlings with the number of blade and number of taking root, the kafir lily seeds cultivation that EMS processes is after 18 months, and strong sprout, rate was up to 90%～95%, and the rate in strong sprout of the undressed matched group of seed is only 27%～30%.
2, eliminate " restraining the growth of seedlings " link, reduce and transplant number of times, reduce cultivation management difficulty, especially moisture, temperature controlled difficulty, be particularly suitable for carrying out the kafir lily strong seedling cultivation of scale in south China area.Its application can not only improve kafir lily seedling quality, increases benefit, and rural area plant structural adjustment and the kafir lily industry development of south China area are significant.
Detailed description of the invention
Below by way of specific embodiment, technical scheme is described in detail.
By processing kafir lily seed with ethylmethane sulfonate prior to seeding in the embodiment of the present invention, and have adjusted method for culturing seedlings to realize effect in strong sprout according to the seedling early growth feature after processing.Specifically comprise the following steps that
The first step: seed selection and seed soaking.Strip seed from through the kafir lily fruit of after-ripening, wash, dry, remove flat grain, select full grains, grow normal, have the seed of eye to be placed in 28 ± 2 DEG C of warm water to soak seed 20 hours, taking-up drains.
Second step: seed treatment.It is 28 ± 2 DEG C that above-mentioned seed is put into temperature, and concentration is in the EMS solution of 0.15～0.2% (v/v), shakes 4～6 hours, then take out on the shaking table that setting speed is 200 revs/min, with running water 4 hours, blots surface moisture standby.The phosphate buffer preparation of described EMS solution 0.1mol/L, pH=6.8.
3rd step: seeding and seedling raising.Seed after processing is sowed according to a conventional method, managed, and substrate can be done with Folium Pini, sawdust, river sand etc. in seedbed, wherein does substrate growing way with Folium Pini best.Within after planting about 20～25 days, going out radicle, leafing in 60～80 days, after 100～120 days, seedling can grow tall to about 1.5 centimetres, and radicle extends to 3～4 centimetres.
4th step: point cultivation seedling.Previous step will grow to seed seedling about 1.5 centimetres high from removal in sowing container, and divide and plant to the earthen basin of bore 10 centimetres, the strain of every basin 3.Dividing and cover thin layer Folium Pini after planting, diseases prevention is antibacterial, increases later stage nutrient simultaneously.Dividing and plant the river sand preparation that substrate adopts the leaf mould of 80% to add 20%, the management after point cultivation is identical with the untreated seedling of onesize seed.
EMS process after seed grow up to the seedling having 4～5 blades and about needed for 1 year from being seeded into, untreated 1～2 blade of plant the youthful and the elderly of the same age of ratio, but shorter strong, glossy dark green, neat, disease resistance is also higher.Due to poor growth in 1 year after the EMS planting seed processed, after point planting, do not need restraining the growth of seedlings, long need not field planting to 4～5 blades.
5th step: field planting forms strong sprout.When the EMS seed seedling processed has grown to 4～5 blades, it is colonizated in the earthen basin of bore 12 centimetres, the strain of every basin 1.Field planting substrate is identical with conventional 1 year raw Seedling with cultivation management measure.After field planting, seedling growth will ramp up, and show feature in strong sprout.
Below by concrete comparative example and embodiment illustrate EMS process after planting seed after there is effect in significant strong sprout.
Comparative example 1
Strip seed from through the Clivia miniata Reg fruit of after-ripening, wash, dry, remove flat grain, select full grains, grow normal, have the seed of eye to be placed in 28 ± 2 DEG C of warm water to soak seed 24 hours, taking-up drains.
Do substrate with Folium Pini, sow management according to a conventional method, within about 60 days, height of seedling 1 centimetre can be grown, radicle length is about the seedling of 4～6 centimetres, seeding ratio about 89.2%.
The seedling of 1 cm divide cultivation to the earthen basin of bore 10 centimetres, the strain of every basin 3.Divide after planting 4 months, carry out field planting when Seedling length is to 2～3 leaf, change a basin later every half a year.Divide cultivation and field planting and change the river sand preparation that basin substrate adopts the leaf mould of 80% to add 20%, and carrying out rich water, illumination and temperature treatment routinely.
By above-mentioned cultivation management, on average can grow 5.7 blades after sowing 1 year, take root 6.1；On average can grow 9.4 blades after 1 year half, take root 12.1.Being respectively greater than meansigma methods (blade 9.4, radical 12.1) as Index of The Healthy Seedlings with the number of blade and number of taking root, kafir lily seed rate in strong sprout after 18 months conventional cultivations is about 27%.
Comparative example 2
The sowing of kafir lily seed and cultivation management and comparative example 1 are essentially identical, only add " restraining the growth of seedlings " link to earthen basin point planting.Namely, after a point basin plant survives, before the 2nd leaf grows, by the moisture Control of basin soil at about 15% 20 day, recover normal cultivation management more later.Same using meet simultaneously the number of blade more than 9.4, number of taking root as relative Index of The Healthy Seedlings, take kafir lily that restraining the growth of seedlings the processes rate in strong sprout after cultivation in 18 months to be about 30% more than 12.1.
Embodiment 1
Strip seed from through the Clivia miniata Reg fruit of after-ripening, wash, dry, remove flat grain, select full grains, grow normal, have the seed of eye to be placed in 28 ± 2 DEG C of warm water to soak seed 20 hours, taking-up drains.
Under above-mentioned same temperature conditions, above-mentioned seed is put in the EMS solution of 0.2% (v/v), the shaking table that setting speed is 200 revs/min shakes 4 hours, then takes out, with running water 4 hours, blot surface moisture standby.The phosphate buffer preparation of described EMS solution 0.1mol/L, pH=6.8.
Do substrate with Folium Pini, the seed after processing is sowed according to a conventional method, managed, height of seedling 1.5 centimetres within 110～120 days, can be grown, radicle length is about the seedling of 3～4 centimetres, seeding ratio about 87.0%.
In the earthen basin divide cultivation receptacle to be bore 10 centimetres high about 1.5 centimetres of seedlings, the strain of every basin 3.Divide after planting, substrate covers thin layer Folium Pini.Divide after planting 8 months, when Seedling length is to 4～5 blades, carry out field planting.Divide the river sand preparation that cultivation and field planting substrate adopt the leaf mould of 80% to add 20%, and carry out rich water, illumination and temperature treatment routinely.
By above-mentioned cultivation management, on average can grow 4.4 blades after sowing 1 year, take root 6.3；On average can grow 11.3 blades after 1 year half, take root 18.2.Using meet simultaneously the number of blade more than 9.4, take root number more than 12.1 as relative Index of The Healthy Seedlings, the kafir lily seed that EMS processes rate in strong sprout after 18 months cultivate is about 95%, shows that growth potential is strong, the feature in strong sprout of short strong, well developed root system, the blade plumpness light of plant.
Embodiment 2
Sowing and the cultivation management of kafir lily seed are substantially the same manner as Example 1, only change concentration for the treatment of and the time of EMS.In the present embodiment, the concentration for the treatment of of EMS is 0.15% (v/v), and the process time is 6 hours.The seeding ratio of the present embodiment about 88.6%, on average can grow 4.8 blades after sowing 1 year, take root 6.0；On average can grow 10.9 blades after 1 year half, take root 16.4.Using meet simultaneously the number of blade more than 9.4, take root number more than 12.1 as Index of The Healthy Seedlings, the kafir lily seed of this process rate in strong sprout after 18 months cultivate is about 90%, shows that growth potential is strong equally, the feature in strong sprout of short strong, well developed root system, the blade plumpness light of plant.
The key data result of above-described embodiment and comparative example is in Table 1.
Table 1:EMS processes the Contrast on effect of seed seedling-raising and conventional seed planting nursery
Note: the CK1 in table 1 represents " comparative example 1 ", CK2 represents " comparative example 2 ".
From the data result of table 1 it can be seen that root, Seedling are all by fast-growth after the seed Seedling field planting of EMS process, after half a year in the amount of taking root achievable pair ratio the 135～150% of seedling of the same age, in number of blade achievable pair ratio the 115～120% of seedling of the same age, show that growth potential is strong, the feature in strong sprout of short strong, well developed root system, the blade plumpness light of plant.Being respectively greater than in comparative example seedling meansigma methods of the same age as Index of The Healthy Seedlings with the number of blade and number of taking root, the seeds cultivation that EMS processes is after 18 months, and strong sprout, rate was up to 90～95%, and the rate only 27～30% in strong sprout of comparative example.
Above are only the part preferred embodiment of the present invention, the present invention is not limited in the content of embodiment.To those skilled in the art, can there be various change and change, any change made and change in the concept of technical solution of the present invention, all within scope.
Claims (5)
1. the method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout, it is characterized in that, the method is: before kafir lily planting seed, described seed is carried out immersion treatment by the ethylmethane sulfonate solution adopting concentration to be 0.15%～0.2% (v/v), after immersion, the ethylmethane sulfonate on seed is rinsed well, then sow；After kafir lily planting seed after described process, carry out a point cultivation in kafir lily seedling length to period 1.2～1.8 centimetres high, after point cultivation, when the kafir lily number of blade reaches 4～5 leaf, carry out field planting.
2. the method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout as claimed in claim 1, it is characterised in that: described ethylmethane sulfonate solution adopts phosphate buffer to prepare.
3. the method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout as claimed in claim 2, it is characterised in that: the concentration of described phosphate buffer is 0.1mol/L, pH value is 6.8.
4. the method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout as claimed in claim 1, it is characterized in that, seed is carried out the condition of immersion treatment by described ethylmethane sulfonate solution: temperature during ethylmethane sulfonate solution soaking is 26～30 DEG C, and soak time is 4～6 hours.
5. the method utilizing ethylmethane sulfonate Fructus Quisqualis orchid strong sprout as claimed in claim 1, it is characterized in that: when seed is soaked by described ethylmethane sulfonate solution, ethylmethane sulfonate solution is positioned on shaking table and makes seed follow solution to shake, to reach to soak uniform purpose.
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| CN101239856A (en) * | 2008-03-10 | 2008-08-13 | 山东金正大生态工程股份有限公司 | Multi-nutrition release-controllable special-purpose fertilizer for clivia and preparation method thereof |
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| CN101239856A (en) * | 2008-03-10 | 2008-08-13 | 山东金正大生态工程股份有限公司 | Multi-nutrition release-controllable special-purpose fertilizer for clivia and preparation method thereof |
Non-Patent Citations (2)
| Title |
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| "EMS对火龙果种子萌发的影响";张冰雪等;《山地农业生物学报》;20130321;第32卷(第3期);第194-197页 * |
| "甲基磺酸乙酯对烤烟种子发芽率的处理效应";王军伟等;《中国烟草科学》;20110630;第32卷(第3期);摘要、第20页第二栏第1段、第18页第1.2节 * |
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