CN104336081A - Zinc thiazole-containing efficient sterilizing and production-increasing composition - Google Patents
Zinc thiazole-containing efficient sterilizing and production-increasing composition Download PDFInfo
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Abstract
The invention discloses a zinc thiazole-containing efficient sterilizing and production-increasing composition which is a sterilizing composition with an active component A and an active component B, wherein the active component A is selected from zinc thiazole and the active component B is selected from any one of the following compounds: brevibacillus laterosporus, bacillus subtilis, bacillus cereus, plant activator proteins, oligosaccharins, bacillus amyloliquefaciens, lentinan and bismerthiazol, and the weight ratio of the components A to B is 1: 100 to 100: 1. The composition disclosed by the invention has relatively high activity to various diseases on various crops and a remarkable synergic effect, expands the bacteriocidal spectrum and meanwhile, effective absorption of fertilizers by crops is increased. The composition is used for helping inferior parts of plants to grow well and adjusting the water balance in the crops, so that the composition has the characteristics of activating the metabolic system of the crops and promoting crop cells to increase and enhancing the root activity.
Description
Technical field
The invention belongs to technical field of pesticide, relate to the application of a kind of efficient sterilizing containing zinc thiazole volume increase composition on corps diseases.
Background technology
Zinc thiazole is a kind of new type bactericide, can be used for preventing and treating multiple diseases, such as mandarin tree canker, rice streak.
Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan or Yekuzuo belong to sterilization well known by persons skilled in the art or plant growth regulator, are widely used in field crop.
But in the real process of agricultural production, the problem that controlling disease the most easily produces is the generation of disease drug resistance.Different cultivars composition carries out composite, is to prevent and treat the very common method of resistance disease.Heterogeneity is carried out composite, according to practical application effect, judge certain composite be synergy, add and or antagonism.In most cases, the composite effect of sterilization is all additive effect, really has the seldom composite of synergistic effect, especially synergistic effect clearly, very high composite just less of synergy ratio.Study through inventor, find by zinc thiazole and Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan or Yekuzuo composite after can produce good synergistic effect, and about zinc thiazole and the composite relevant report of Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan or Yekuzuo not yet open.
For target plant, plant growth regulator is the non-nutritive chemical substance of external source, usually can conduct to site of action in plant corpus, just can promote or suppress some link of its life process with very low concentration, makes it the needs development to meeting the mankind.Every plant growth regulators has specific purposes, and application technology requires quite strict, only could produce specific effect to target plant under specific application conditions (comprising extraneous factor).Often change concentration and will obtain contrary result, such as, have facilitation at low concentrations, then become inhibitory action in higher concentrations.Active components A is different from the active component B mechanism of action, mutually composite have good synergistic effect within the specific limits, and about the binary built of zinc thiazole and bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, there is not yet relevant report at home and abroad at present.
Summary of the invention
The object of the invention is to propose a kind of to there is the volume increase of the efficient sterilizing containing the zinc thiazole composition that synergistic function, use cost are low, preventive effect is good.
The volume increase of the efficient sterilizing containing the zinc thiazole composition that the present invention proposes contains active components A and active component B, active components A and active component B weight ratio are 1:100 ~ 100:1, described active components A is selected from zinc thiazole, active component B is selected from the one in Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan or Yekuzuo, and active components A and the preferred weight ratio of active component B are 1:70 ~ 70:1; The weight ratio being more preferably zinc thiazole and Brevibacillus laterosporus is 1:25 ~ 30:1, the weight ratio of zinc thiazole and bacillus subtilis is 1:30 ~ 25:1, the weight ratio of zinc thiazole and waxy Bacillus is 1:30 ~ 25:1, the weight ratio of zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is 1:25 ~ 30:1, the weight ratio of zinc thiazole and amino-oligosaccharide is 1:20 ~ 35:1, the weight ratio of zinc thiazole and bacillus amyloliquefaciens is 1:30 ~ 25:1, the weight ratio of zinc thiazole and lentinan is 1:15 ~ 40:1, and the weight ratio of zinc thiazole and Yekuzuo is 1:30 ~ 30:1; The weight ratio most preferably being zinc thiazole and Brevibacillus laterosporus is 1:9 ~ 10:1, the weight ratio of zinc thiazole and bacillus subtilis is 1:11 ~ 6:1, the weight ratio of zinc thiazole and waxy Bacillus is 1:10 ~ 9:1, the weight ratio of zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is 1:6 ~ 11:1, the weight ratio of zinc thiazole and amino-oligosaccharide is 1:1 ~ 15:1, the weight ratio of zinc thiazole and bacillus amyloliquefaciens is 1:11 ~ 6:1, the weight ratio of zinc thiazole and lentinan is 1:1 ~ 25:1, and the weight ratio of zinc thiazole and Yekuzuo is 1:9 ~ 9:1.
The volume increase of the efficient sterilizing containing the zinc thiazole composition that the present invention proposes is for preventing and treating the purposes of disease on crops, and described crops comprise cereal crops, legume crop, fiber crop, sugar [yielding, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flower crop, medicinal crop, crop, green manure pasture crop; Described disease is canker, cecospora spot, phytophthora root rot, fusarium wilt, head blight, gray mold, powdery mildew, bacterial wilt, soft rot, virus disease, epidemic disease, anthracnose, scab, damping off, root rot, downy mildew or leaf blight.
The weight ratio of active components A, active component B is 1:100 ~ 100:1.In usual composition, the weight percentage of active component is 0.5% ~ 90% of gross weight, is preferably 5% ~ 80%.According to different preparation types, active component content scope is different.Usually, liquid preparation contains the active substance of by weight 1% ~ 70%, is preferably 5% ~ 50%; Solid pharmaceutical preparation contains the active substance of by weight 5% ~ 80%, is preferably 10% ~ 80%.
At least containing a kind of surfactant in bactericidal composition of the present invention, the dispersion of active component in water when being beneficial to use.Surface-active contents is 5% ~ 30% of total formulation weight amount, and surplus is solid or liquid diluent.
Bactericidal composition of the present invention can by user before use through diluting or directly using.Its preparation can be prepared by common processing method known in those skilled in the art, after mixing with liquid flux or solid carrier by active substance, then add surfactant as one or more in dispersant, stabilizing agent, wetting agent, binding agent, defoamer, disintegrant, antifreeze etc.
Bactericidal composition of the present invention, acceptable formulation in any sterilization can be processed on demand, wherein more preferably formulation is wetting powder, water dispersible granules, suspending agent, granule, can also make aqua, seed coat agent, microemulsion, microcapsule suspending agent, suspension emulsion, oil-suspending agent, microcapsule suspension-suspendinagent agent.
Composition comprises following constituent content when making wetting powder: active components A 0.1% ~ 80%, active component B0.01% ~ 80%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
Composition comprises following constituent content when making water dispersible granules: active components A 0.1% ~ 80%, active component B0.01% ~ 80%, dispersant 3% ~ 12%, wetting agent 1% ~ 8%, disintegrant 1% ~ 10%, binding agent 0 ~ 8%, filler surplus.
Composition comprises following constituent content when making suspending agent: active components A 0.1% ~ 50%, active component B0.01% ~ 50%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, defoamer 0.01% ~ 2%, thickener 0 ~ 2%, antifreeze 0 ~ 8%, deionized water add to 100%.
Composition comprises following constituent content when making granule: active components A 0.1% ~ 80%, active component B0.01% ~ 80%, binding agent 0 ~ 8%, colouring agent 0.1%-2%, dispersant 2% ~ 10%, wetting agent 2% ~ 10%, filler surplus.
Wetting powder the key technical indexes of the present invention:
Water dispersible granules the key technical indexes of the present invention:
Suspending agent the key technical indexes of the present invention:
Granule the key technical indexes of the present invention:
The invention has the advantages that:
(1) zinc thiazole and Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan or Yekuzuo composite after, there is obvious synergy and lasting effect; (2) all greater activity is had to the disease on cereal crops, legume crop, fiber crop, sugar [yielding, melon crop, fruits crop, dry fruit crop, hobby crop, root crop, oil crop, flower crop, medicinal crop, crop, green manure pasture crop; (3) decrease pesticide usage amount, reduce the residual quantity of agricultural chemicals on crop, alleviate environmental pollution; (4) to person poultry safety, Environmental compatibility is good; And preparation adhesion strength strengthens, resistance of rainwater washing against; (5) obviously have activated the metabolic system of crop, promote that crop cell increases, improving activity of root system is strengthened.
Embodiment
Below in conjunction with embodiment to further instruction of the present invention, the percentage in embodiment is weight ratio, but the present invention is not limited thereto.
Application Example one
Embodiment 1 ~ 24 wetting powder
Zinc thiazole, active component B, dispersant, wetting agent, filler are mixed in mixed cylinder, mixes again after airslide disintegrating mill is pulverized, wetting powder product of the present invention can be obtained, specifically in table 1.
The each component of table 1 embodiment 1 ~ 24 and weight portion
Embodiment 25 ~ 48 water dispersible granules
Zinc thiazole, active component B, dispersant, wetting agent, disintegrant, filler etc. are obtained together the particle diameter needed through air-flow crushing, then add other auxiliary agents such as binding agent (can add and can not add), obtain granulation materials.Item is quantitatively sent in fluidized bed granulating dryer through granulating and after drying, water dispersible granules product of the present invention can being obtained, specifically in table 2.
The each component of table 2 embodiment 25 ~ 48 and weight portion
Embodiment 49 ~ 72 suspending agent
By dispersant, wetting agent, defoamer, thickener (can add and can not add), antifreeze (can add and can not add), mix through high speed shear, add zinc thiazole, active component B, ball milling 2 ~ 3 hours in ball mill, make diameter of particle all below 5 μm, surplus deionized water is supplied, and can obtain suspending agent product of the present invention, specifically in table 3.
The each component of table 3 embodiment 49 ~ 72 and weight portion
Embodiment 73 ~ 88 granule
Zinc thiazole, active component B, binding agent, colouring agent, dispersant, wetting agent, filler are mixed in mixed cylinder, through extruder grain, drying, and through sieving obtained granule product of the present invention, specifically in table 4.
The each component of table 4 embodiment 73 ~ 88 and weight portion
Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan, Yekuzuo in table 1 ~ 4 are exchanged, can novel formulation be obtained.
This measuring adopts growth of seedling method.Determination step is as follows:
Selecting seed of the same size dials in the culture dish of the 9cm size being covered with two layers of filter paper respectively, and 10, every ware adds the liquid 1.5ml configured, and contrast adding distil water, repeats 4 times.Cover ware lid, the artificial climate being placed into 28 ± 1 DEG C is indoor, illumination 10 hours.In 6 days check heights of seedling and root long.
Result statistical analysis:
The embodiment of the present invention is the method adopting Toxicity Determination and field trial to combine.First by Toxicity Determination, the synergy ratio (SR) after clear and definite two kinds of medicaments are composite by a certain percentage, SR < 0.5 is antagonism, 0.5≤SR≤1.5 are summation action, SR > 1.5 is synergistic effect, on this basis, then carries out field trial.
Test method: determine that after the effective inhibition concentration scope of each medicament, medicament establishes 5 dosage process respectively by active constituent content through prerun, if clear water contrast.Carry out with reference to " farm-chemical indoor determination test rule bactericide ", adopt mycelial growth rate method to measure medicament to the virulence of crop germ.Measure colony diameter by right-angled intersection method after 72h, calculate each process net growth, mycelial growth inhibition rate.
Net growth (mm)=measurement colony diameter-5
Mycelial growth inhibition rate is converted into probit value (y), and liquor strength (μ g/mL) converts logarithm value to (x), tries to achieve virulence regression equation (y=a+bx), and calculate the EC of often kind of medicament thus with method of least squares
50value.Calculate two medicament different ratio synergies ratio (SR) according to Wadley method, SR < 0.5 is antagonism, and 0.5≤SR≤1.5 are summation action simultaneously, and SR > 1.5 is synergistic effect.Computing formula is as follows:
Wherein: a, b are respectively active components A and the ratio of active component B shared by combination;
A is zinc thiazole;
B is selected from the one in Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan, Yekuzuo.
Application Example two:
For examination disease: mandarin tree canker
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of Brevibacillus laterosporus and the two different ratio mixture.
Toxicity test result
Table 5 zinc thiazole and the composite toxicity test analysis of results table to mandarin tree canker of Brevibacillus laterosporus
As shown in Table 5, zinc thiazole and Brevibacillus laterosporus proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of mandarin tree canker, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and Brevibacillus laterosporus is at 1 ︰ 25 ~ 30 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.25; When the proportioning of zinc thiazole and Brevibacillus laterosporus is at 1 ︰ 9 ~ 10 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.40.Testing through applicant the optimum ratio finding zinc thiazole and Brevibacillus laterosporus is 30:1,25:1,20:1,15:1,12:1,10:1,8:1,6:1,5:1,4:1,3:4,2:1,3:2,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:10,1:15,1:20,1:25, especially when zinc thiazole and Brevibacillus laterosporus weight ratio are 3:2, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example three
For examination disease: powdery mildew of cucumber
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of bacillus subtilis and the two different ratio mixture.
Table 6 zinc thiazole and the composite toxicity test analysis of results table to powdery mildew of cucumber of bacillus subtilis
As shown in Table 6, zinc thiazole and bacillus subtilis proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of powdery mildew of cucumber, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and bacillus subtilis is at 1 ︰ 30 ~ 25 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.30; When the proportioning of zinc thiazole and bacillus subtilis is at 1 ︰ 11 ~ 6 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.50.Testing through applicant the optimum ratio finding zinc thiazole and bacillus subtilis is 25:1,20:1,15:1,12:1,10:1,8:1,6:1,5:1,4:1,3:4,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:10,1:15,1:20,1:25,1:30, especially when zinc thiazole and bacillus subtilis weight ratio are 1:2, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example four
For examination disease: eggplant bacterial wilt
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of waxy Bacillus and the two different ratio mixture.
Table 7 zinc thiazole and the composite toxicity test analysis of results table to eggplant bacterial wilt of waxy Bacillus
As shown in Table 7, zinc thiazole and waxy Bacillus proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of eggplant bacterial wilt, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and waxy Bacillus is at 1 ︰ 30 ~ 25 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.30; When the proportioning of zinc thiazole and waxy Bacillus is at 1 ︰ 10 ~ 9 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.50.Testing through applicant the optimum ratio finding zinc thiazole and waxy Bacillus is 25:1,20:1,15:1,12:1,10:1,8:1,6:1,5:1,4:1,3:4,2:1,1:1,2:3,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:10,1:15,1:20,1:25,1:30, especially when zinc thiazole and waxy Bacillus weight ratio are 2:3, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example five
For examination disease: rice streak
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of toVerticilliumdahliaActivitie Activitie S of Relative activator protein and the two different ratio mixture.
Table 8 zinc thiazole and the composite toxicity test analysis of results table to rice streak of toVerticilliumdahliaActivitie Activitie S of Relative activator protein
As shown in Table 8, zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of rice streak, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is at 1 ︰ 25 ~ 30 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.25; When the proportioning of zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is at 1 ︰ 6 ~ 11 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.35.Testing through applicant the optimum ratio finding zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is 30:1,25:1,20:1,15:1,12:1,10:1,8:1,6:1,5:1,4:1,3:4,2:1,1:1,2:3,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:10,1:15,1:20,1:25, especially when zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein weight ratio are 2:1, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example six
For examination disease: tomato virus disease
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of amino-oligosaccharide and the two different ratio mixture.
Table 9 zinc thiazole and the composite toxicity test analysis of results table to tomato virus disease of amino-oligosaccharide
As shown in Table 9, zinc thiazole and amino-oligosaccharide proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of tomato virus disease, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and amino-oligosaccharide is at 1 ︰ 20 ~ 35 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.20; When the proportioning of zinc thiazole and amino-oligosaccharide is at 1 ︰ 1 ~ 15 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.40.Testing through applicant the optimum ratio finding zinc thiazole and amino-oligosaccharide is 35:1,30:1,25:1,20:1,18:1,15:1,12:1,11:1,10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,1:1,1:2,1:5,1:10,1:15,1:20, especially when zinc thiazole and amino-oligosaccharide weight ratio are 7:1, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example seven
For examination disease: downy mildew of garpe
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of bacillus amyloliquefaciens and the two different ratio mixture.
Table 10 zinc thiazole and the composite toxicity test analysis of results table to downy mildew of garpe of bacillus amyloliquefaciens
As shown in Table 10, zinc thiazole and bacillus amyloliquefaciens proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of downy mildew of garpe, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and bacillus amyloliquefaciens is at 1 ︰ 30 ~ 25 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.25; When the proportioning of zinc thiazole and bacillus amyloliquefaciens is at 1 ︰ 11 ~ 6 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.40.Testing through applicant the optimum ratio finding zinc thiazole and bacillus amyloliquefaciens is 25:1,20:1,15:1,12:1,10:1,8:1,6:1,5:1,4:1,3:4,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:10,1:15,1:20,1:25,1:30, especially when zinc thiazole and bacillus amyloliquefaciens weight ratio are 1:2, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example eight
For examination disease: Rice Leaf rot
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of lentinan and the two different ratio mixture.
Table 11 zinc thiazole and the composite toxicity test analysis of results table to Rice Leaf rot of lentinan
As shown in Table 11, zinc thiazole and lentinan proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of Rice Leaf rot, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and lentinan is at 1 ︰ 15 ~ 40 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.20; When the proportioning of zinc thiazole and lentinan is at 1 ︰ 1 ~ 25 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.40.Testing through applicant the optimum ratio finding zinc thiazole and lentinan is 40:1,35:1,30:1,25:1,20:1,19:1,18:1,17:1,16:1,15:1,14:1,13:1,12:1,11:10,8:1,5:1,4:1,1:1,1:2,1:5,1:10,1:15, especially when zinc thiazole and lentinan weight ratio are 14:1, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example nine
For examination disease: Rice Leaf rot
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through effective inhibition concentration scope of preliminary experiment determination zinc thiazole and the former medicine of Yekuzuo and the two different ratio mixture.
Table 12 zinc thiazole and the composite toxicity test analysis of results table to Rice Leaf rot of Yekuzuo
As shown in Table 12, zinc thiazole and Yekuzuo proportioning are all greater than 1.5 when 1 ︰ 100 ~ 100 ︰ 1 to the synergy ratio SR of Rice Leaf rot, both explanations mixture in 1 ︰ 100 ~ 100 ︰ 1 scope all shows synergistic effect, when the proportioning of zinc thiazole and Yekuzuo is at 1 ︰ 30 ~ 30 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.25; When the proportioning of zinc thiazole and Yekuzuo is at 1 ︰ 9 ~ 9 ︰ 1, synergistic effect is more outstanding, and synergy ratio is all more than 2.40.Testing through applicant the optimum ratio finding zinc thiazole and Yekuzuo is 30:1,25:1,20:1,15:1,12:1,10:1,8:1,6:1,5:1,4:1,3:4,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:10,1:15,1:20,1:25,1:30, especially when zinc thiazole and Yekuzuo weight ratio are 1:1, synergy ratio is maximum, and synergistic effect is the most obvious.
Application Example ten
For studying thing: tomato
Test medicine provides by Shaanxi Meibang Pesticide Co., Ltd..
Experimental scheme: through the concentration range of both preliminary experiment determination zinc thiazole and active component B different ratio mixture coordinate plant growth.
The mixed liquid of table 13 zinc thiazole and active component B and different proportion thereof is to the growth regulation effect of tomato
As shown in Table 13, zinc thiazole and active component B used in combination after, can activated plant metabolic system and make plant growth accelerate vigor strengthen, promote that plant cell increases, improving activity of root system is strengthened, reach volume increase object, zinc thiazole and active component B, when 1 ︰ 100 ~ 100 ︰ 1, all have stronger regulating action.
Through test find: zinc thiazole and active component B composite after have obvious synergistic effect to the control of the canker in various crop, cecospora spot, phytophthora root rot, fusarium wilt, head blight, gray mold, powdery mildew, bacterial wilt, soft rot, virus disease, epidemic disease, anthracnose, scab, damping off, root rot, downy mildew or leaf blight, synergy ratio SR is all greater than 1.5.
Effect experiment part: test medicine is researched and developed by Shaanxi Meibang Pesticide Co., Ltd., provided.
Table 14 test medicine
Application Example 11 zinc thiazole and fractions of active ingredient B and composite water prevention Cercospora spot drug effect thereof and growth regulation Experiment on Function
This test arrangement is in Hanzhong City suburb, Shaanxi Province, and investigation rice streak disease index before medicine, in the dispenser of disease initial stage of origination, 5 days, 15 days, 30 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 15 zinc thiazole and fractions of active ingredient B composite water prevention Cercospora spot drug effect and growth regulation Experiment on Function
As can be seen from Table 15, zinc thiazole and fractions of active ingredient B composite after can effective water prevention Cercospora spot, through applicant test find zinc thiazole and fractions of active ingredient B composite after also can effectively prevent and treat paddy rice leaf blight, leaf blight, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example ten dithiazole zinc and fractions of active ingredient B and compositely prevent and treat wheat scab drug effect and growth regulation Experiment on Function
This test arrangement is in Weinan City Pucheng County, Shaanxi Province, and investigation wheat scab disease index before medicine, in the dispenser of disease initial stage of origination, 3 days, 10 days, 20 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 16 zinc thiazole and fractions of active ingredient B and compositely prevent and treat wheat scab drug effect and growth regulation Experiment on Function
As can be seen from Table 16, zinc thiazole and fractions of active ingredient B composite after effectively can prevent and treat wheat scab, through applicant test find zinc thiazole and fractions of active ingredient B composite after also effectively can prevent and treat root rotof flax, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 13 zinc thiazole and fractions of active ingredient B and composite control pepper virus disease drug effect thereof and growth regulation Experiment on Function
This test arrangement is at Xian District, Shanxi Province suburban area, and investigation pepper virus disease disease index before medicine, in the dispenser of disease initial stage of origination, 5 days, 15 days, 30 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 17 zinc thiazole and fractions of active ingredient B and composite control pepper virus disease drug effect thereof and growth regulation Experiment on Function
As can be seen from Table 17, zinc thiazole and fractions of active ingredient B and composite control pepper virus disease thereof, through applicant test find zinc thiazole and fractions of active ingredient B composite after also can effectively mixed with rice bran, epidemic disease, phytophthora root rot, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 14 zinc thiazole and fractions of active ingredient B and composite control watermelon blight drug effect thereof and growth regulation Experiment on Function
This test arrangement is in Weinan City Dali County, Shaanxi Province, and investigation watermelon blight disease index before medicine, in the dispenser of disease initial stage of origination, 3 days, 10 days, 20 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 18 zinc thiazole and fractions of active ingredient B composite control watermelon blight drug effect and growth regulation Experiment on Function
As can be seen from Table 18, zinc thiazole and fractions of active ingredient B composite after effectively can prevent and treat watermelon blight, through applicant test find zinc thiazole and fractions of active ingredient B composite after also can effectively prevent and treat watermelon powdery mildew, fusarium wilt, epidemic disease, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.Application Example 15 zinc thiazole and fractions of active ingredient B and composite control citrus bacterial canker disease drug effect thereof and growth regulation Experiment on Function
This test arrangement is in Hanzhong City suburb, Shaanxi Province, and investigation citrus bacterial canker disease disease index before medicine, in the dispenser of disease initial stage of origination, 10 days, 20 days, 30 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 19 zinc thiazole and fractions of active ingredient B composite control citrus bacterial canker disease drug effect and growth regulation Experiment on Function
As can be seen from Table 19, zinc thiazole and fractions of active ingredient B composite after effectively can prevent and treat citrus bacterial canker disease, through applicant test find zinc thiazole and fractions of active ingredient B composite after also effectively can prevent and treat citrus anthracnose, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 16 zinc thiazole and fractions of active ingredient B and compositely prevent and treat downy mildew of garpe drug effect and growth regulation Experiment on Function
This test arrangement is in Weinan City Dali County, Shaanxi Province, and investigation downy mildew of garpe disease index before medicine, in the dispenser of disease initial stage of origination, 7 days, 14 days, 25 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 20 zinc thiazole and fractions of active ingredient B is composite prevents and treats downy mildew of garpe drug effect and growth regulation Experiment on Function
As can be seen from Table 20, zinc thiazole and fractions of active ingredient B composite after effectively can prevent and treat downy mildew of garpe, through applicant test find zinc thiazole and fractions of active ingredient B composite after also effectively can prevent and treat grape powdery mildew, control efficiency is all higher than 96%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 17 zinc thiazole and fractions of active ingredient B and composite control apple tree powdery mildew drug effect thereof and growth regulation Experiment on Function
This test arrangement is at Shaanxi Province's Weinan City Baishui County, and investigation apple tree powdery mildew disease index before medicine, in the dispenser of disease initial stage of origination, 7 days, 14 days, 25 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 21 zinc thiazole and fractions of active ingredient B is composite prevents and treats apple tree powdery mildew drug effect and growth regulation Experiment on Function
As can be seen from Table 21, zinc thiazole and fractions of active ingredient B composite after can effectively preventing and treating apple-tree powdery mildew, through applicant test find zinc thiazole and fractions of active ingredient B composite after also effectively can prevent and treat apple scab, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
Application Example 18 zinc thiazole and fractions of active ingredient B and composite control pear scab drug effect thereof and growth regulation Experiment on Function
This test arrangement is in Weinan City suburb, Shaanxi Province, and investigation pear scab disease index before medicine, in the dispenser of disease initial stage of origination, 7 days, 14 days, 25 days investigation disease indexes after dispenser also calculate preventive effect, the growth rate of second time dispenser " Invest, Then Investigate " crop.Result of the test is as follows:
Table 22 zinc thiazole and fractions of active ingredient B and composite control pear scab drug effect thereof and growth regulation Experiment on Function
As can be seen from Table 22, zinc thiazole and fractions of active ingredient B composite after effectively can prevent and treat pear scab, through applicant test find zinc thiazole and fractions of active ingredient B composite after also can effectively prevent and treat pear tree anthracnose, control efficiency is all higher than 95%, be better than the preventive effect of single dose, the preventive effect phase is long, and can activated plant metabolic system and make plant growth accelerate vigor to strengthen, strengthen the growth of root system.In test scope of medication, target crop is had no adverse effects simultaneously.
After draw through tests different local throughout the country, zinc thiazole and Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan, Yekuzuo is composite rear to the canker in various crop, cecospora spot, phytophthora root rot, fusarium wilt, head blight, gray mold, powdery mildew, bacterial wilt, soft rot, virus disease, epidemic disease, anthracnose, scab, damping off, root rot, the preventive effect of the Common Diseases such as downy mildew or leaf blight is all more than 95%, be better than single dose preventive effect, synergistic effect is obvious.
Claims (6)
1. the volume increase of the efficient sterilizing containing a zinc thiazole composition, effective active composition is active components A and active component B, it is characterized in that: active components A and active component B weight ratio are 1:100 ~ 100:1, described active components A is selected from zinc thiazole, and active component B is selected from the one in Brevibacillus laterosporus, bacillus subtilis, waxy Bacillus, toVerticilliumdahliaActivitie Activitie S of Relative activator protein, amino-oligosaccharide, bacillus amyloliquefaciens, lentinan or Yekuzuo.
2. the volume increase of the efficient sterilizing containing zinc thiazole composition according to claim 1, is characterized in that: the weight ratio of active components A and active component B is 1:70 ~ 70:1.
3. the volume increase of the efficient sterilizing containing zinc thiazole composition according to claim 2, is characterized in that:
The weight ratio of zinc thiazole and Brevibacillus laterosporus is 1:25 ~ 30:1;
The weight ratio of zinc thiazole and bacillus subtilis is 1:30 ~ 25:1;
The weight ratio of zinc thiazole and waxy Bacillus is 1:30 ~ 25:1;
The weight ratio of zinc thiazole and toVerticilliumdahliaActivitie Activitie S of Relative activator protein is 1:25 ~ 30:1;
The weight ratio of zinc thiazole and amino-oligosaccharide is 1:20 ~ 35:1;
The weight ratio of zinc thiazole and bacillus amyloliquefaciens is 1:30 ~ 25:1;
The weight ratio of zinc thiazole and lentinan is 1:15 ~ 40:1;
The weight ratio of zinc thiazole and Yekuzuo is 1:30 ~ 30:1.
4. the volume increase of the efficient sterilizing containing zinc thiazole composition according to any one of claim 1 to 3, is characterized in that: composition makes wetting powder, water dispersible granules, suspending agent or granule.
5. the volume increase of the efficient sterilizing containing zinc thiazole composition according to claim 4 is for preventing and treating the purposes of crop pest and Promoting plant growth.
6. purposes according to claim 5, is characterized in that: described disease is canker, cecospora spot, phytophthora root rot, fusarium wilt, head blight, gray mold, powdery mildew, bacterial wilt, soft rot, virus disease, epidemic disease, anthracnose, scab, damping off, root rot, downy mildew or leaf blight.
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| CN104222107A (en) * | 2014-08-26 | 2014-12-24 | 广东中迅农科股份有限公司 | Pesticide composition containing zinc thiazole and bismerthiazol |
| CN105284899A (en) * | 2015-10-16 | 2016-02-03 | 联保作物科技有限公司 | Pesticide composition, application thereof and preparation thereof |
| CN106561720A (en) * | 2016-10-11 | 2017-04-19 | 江苏辉丰农化股份有限公司 | Sterilization composition containing benzoisothiazolinone and plant activator protein |
| CN107518006A (en) * | 2017-08-25 | 2017-12-29 | 河南中麦生物科技有限公司 | A kind of bacterium solution extremely brought back to life for vegetables and fruit leaf and its application |
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| CN111748496A (en) * | 2020-07-03 | 2020-10-09 | 天津坤禾生物科技集团股份有限公司 | Application of Brevibacillus laterosporus MES818 in tomato cultivation |
| CN113508786A (en) * | 2021-08-04 | 2021-10-19 | 刘梦然 | Application of bacillus subtilis in bee-keeping industry |
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| CN111748496A (en) * | 2020-07-03 | 2020-10-09 | 天津坤禾生物科技集团股份有限公司 | Application of Brevibacillus laterosporus MES818 in tomato cultivation |
| CN111748496B (en) * | 2020-07-03 | 2022-05-13 | 天津坤禾生物科技集团股份有限公司 | Application of Brevibacillus laterosporus MES818 in tomato cultivation |
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