CN104324058A - Preparation method of ganoderma lucidum spores oil - Google Patents
Preparation method of ganoderma lucidum spores oil Download PDFInfo
- Publication number
- CN104324058A CN104324058A CN201310623484.0A CN201310623484A CN104324058A CN 104324058 A CN104324058 A CN 104324058A CN 201310623484 A CN201310623484 A CN 201310623484A CN 104324058 A CN104324058 A CN 104324058A
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- Prior art keywords
- preparation
- ganoderma spore
- oil
- enzyme liquid
- ganoderma lucidum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of ganoderma lucidum spores oil, and belongs to the technical field of extraction of effective components from medicinal plants. The preparation method of the ganoderma lucidum spores oil comprises the steps of breaking cell walls of ganoderma lucidum spores by using an enzymolysis method; then carrying out wet granulation by using water as a solvent; extracting with supercritical CO2; centrifuging and refining to obtain a light yellow oily material being the ganoderma lucidum spores oil. The preparation method overcomes the problems that active substances in the spores are difficult to extract due to double-layer cell walls of the ganoderma lucidum spores. With the preparation method provided by the invention, the yield of the ganoderma lucidum spores oil is higher than 30%.
Description
Technical field
The present invention relates to active ingredient of autonomic drug extractive technique field, especially a kind of preparation method of Ganoderma spore oil.
Background technology
Ganoderma spore is the dry spore of Polyporaceae (Ployporaceae) Ganoderma Ganoderma Lucium (Ley S.ex Fr.) Karest, for collecting when tame Ganoderma bullet puts spore, drying and obtaining.Ganoderma spore has whole genetic activities materials of Ganoderma, is the elite of Ganoderma.Ganoderma spore oil extracts from Ganoderma spore, and its main component is triterpenes Ganodenic acid, unsaturated fatty acid, organic germanium and a small amount of ganoderan.
Ganoderma spore has double-layer cell wall, its main component is chitin, lignin and cellulose, Si, Ca, Fe, Mg, Al etc., make sporoderm very solid hard, can acidproof, alkaline-resisting, withstand voltage, heatproof, also highly stable to digestive enzyme, in spore, active substance is wrapped and not easily extracts and be prepared into Ganoderma spore oil.
Summary of the invention
The object of this invention is to provide one can be quick, the preparation method of efficient Ganoderma spore oil, solve because Ganoderma spore has the existence of double-layer cell wall and make the problem that active substance in spore is not easily extracted, the yield of its Ganoderma spore oil reaches more than 30%.
The technical scheme of technical solution problem of the present invention is:
A preparation method for Ganoderma spore oil, comprises the following steps:
Being raw material with Ganoderma spore, through enzymatic shell-broken, take water as solvent wet granulation, supercritical CO
2after extraction, centrifuge refining, obtain pale yellow oil i.e. this Ganoderma spore oil;
Wherein, described enzymatic shell-broken process is specially: first Ganoderma spore is immersed in 75% ethanol the 15min ~ 20min that carries out disinfection, and then puts into enzyme liquid enzymolysis 18h ~ 24h; Described enzyme digestion reaction carries out at dark place; The temperature of described enzyme digestion reaction is 28 DEG C ~ 31 DEG C; Described enzyme liquid cellulase by account for enzyme liquid gross mass 2% ~ 5%, sucrose account for enzyme liquid gross mass 12% ~ 16%, inorganic salt accounts for 0.5% ~ 0.8% of enzyme liquid gross mass, remaining is made up of buffer.
Owing to adopting technique scheme, the beneficial effect that the present invention obtains is:
The cell wall of Ganoderma spore in the present invention, does not need to adopt Mechanical Crushing, saves and buy the expense of main equipment and the human and material resources spent by Mechanical Crushing, but adopt enzymatic isolation method to destroy its cell wall, cost is low, effective, and the yield of its Ganoderma spore oil reaches more than 30%.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1
The preparation method of Ganoderma spore oil comprises the following steps:
1Kg Ganoderma spore is immersed in 75% ethanol the 15min that carries out disinfection, put into by the cellulase accounting for enzyme liquid gross mass 2% again, account for the sucrose of enzyme liquid gross mass 12%, account for the inorganic salt of enzyme liquid gross mass 0.5%, to account in the enzyme liquid of the buffer composition of enzyme liquid gross mass 85.5% and to be placed in dark place, be enzymolysis 18h under the condition of 28 DEG C in temperature, then filter and be solvent wet granulation with water, supercritical CO
2after extraction, centrifuge refining, obtain 0.31Kg pale yellow oil i.e. this Ganoderma spore oil.
In the present embodiment, the yield of Ganoderma spore oil is 31%.
Embodiment 2
The preparation method of Ganoderma spore oil comprises the following steps:
1Kg Ganoderma spore is immersed in 75% ethanol the 20min that carries out disinfection, put into by the cellulase accounting for enzyme liquid gross mass 3% again, account for the sucrose of enzyme liquid gross mass 14%, account for the inorganic salt of enzyme liquid gross mass 0.6%, to account in the enzyme liquid of the buffer composition of enzyme liquid gross mass 82.4% and to be placed in dark place, be enzymolysis 124h under the condition of 31 DEG C in temperature, then filter and be solvent wet granulation with water, supercritical CO
2after extraction, centrifuge refining, obtain 0.35Kg pale yellow oil i.e. this Ganoderma spore oil.
In the present embodiment, the yield of Ganoderma spore oil is 35%.
Embodiment 3
The preparation method of Ganoderma spore oil comprises the following steps:
1Kg Ganoderma spore is immersed in 75% ethanol the 18min that carries out disinfection, put into by the cellulase accounting for enzyme liquid gross mass 5% again, account for the sucrose of enzyme liquid gross mass 16%, account for the inorganic salt of enzyme liquid gross mass 0.8%, to account in the enzyme liquid of the buffer composition of enzyme liquid gross mass 78.2% and to be placed in dark place, be enzymolysis 21h under the condition of 30 DEG C in temperature, then filter and be solvent wet granulation with water, supercritical CO
2after extraction, centrifuge refining, obtain 0.404Kg pale yellow oil i.e. this Ganoderma spore oil.
In the present embodiment, the yield of Ganoderma spore oil is 40.4%.
Claims (1)
1. a preparation method for Ganoderma spore oil, comprising with Ganoderma spore is raw material, through enzymatic shell-broken, take water as solvent wet granulation, supercritical CO
2after extraction, centrifuge refining, obtain pale yellow oil i.e. this Ganoderma spore oil; It is characterized in that described enzymatic shell-broken process is specially: first Ganoderma spore is immersed in 75% ethanol the 15min ~ 20min that carries out disinfection, and then put into enzyme liquid enzymolysis 18h ~ 24h; Described enzyme digestion reaction carries out at dark place; The temperature of described enzyme digestion reaction is 28 DEG C ~ 31 DEG C; Described enzyme liquid cellulase by account for enzyme liquid gross mass 2% ~ 5%, sucrose account for enzyme liquid gross mass 12% ~ 16%, inorganic salt accounts for 0.5% ~ 0.8% of enzyme liquid gross mass, remaining is made up of buffer.
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CN201310623484.0A CN104324058A (en) | 2013-11-30 | 2013-11-30 | Preparation method of ganoderma lucidum spores oil |
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CN201310623484.0A CN104324058A (en) | 2013-11-30 | 2013-11-30 | Preparation method of ganoderma lucidum spores oil |
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CN201310623484.0A Pending CN104324058A (en) | 2013-11-30 | 2013-11-30 | Preparation method of ganoderma lucidum spores oil |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106867657A (en) * | 2017-03-17 | 2017-06-20 | 广州康琪莱生物科技有限公司 | A kind of efficient, multi-stage supercritical CO2The method of Ganoderma Lucidum Spores Oil Extracted |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1165032A (en) * | 1996-05-10 | 1997-11-19 | 中国科学院大连化学物理研究所 | Method for breaking cell wall of Ganoderma lucidum spore |
CN1262957A (en) * | 1999-11-19 | 2000-08-16 | 中国科学院南京地理与湖泊研究所科技开发公司 | Supercritical processing method of ganoderma spore |
US20020131978A1 (en) * | 2001-03-19 | 2002-09-19 | Xin Liu | Method for extracting oleaginous substances from ganoderma lucidum spores |
CN1883590A (en) * | 2006-05-24 | 2006-12-27 | 广东粤微食用菌技术有限公司 | Method for preparing ganoderma spore oil |
CN101381716A (en) * | 2008-10-23 | 2009-03-11 | 肇东市日成酶制剂有限公司 | Composite stabilizer for preservation of liquid cellulase |
-
2013
- 2013-11-30 CN CN201310623484.0A patent/CN104324058A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1165032A (en) * | 1996-05-10 | 1997-11-19 | 中国科学院大连化学物理研究所 | Method for breaking cell wall of Ganoderma lucidum spore |
CN1262957A (en) * | 1999-11-19 | 2000-08-16 | 中国科学院南京地理与湖泊研究所科技开发公司 | Supercritical processing method of ganoderma spore |
US20020131978A1 (en) * | 2001-03-19 | 2002-09-19 | Xin Liu | Method for extracting oleaginous substances from ganoderma lucidum spores |
CN1883590A (en) * | 2006-05-24 | 2006-12-27 | 广东粤微食用菌技术有限公司 | Method for preparing ganoderma spore oil |
CN101381716A (en) * | 2008-10-23 | 2009-03-11 | 肇东市日成酶制剂有限公司 | Composite stabilizer for preservation of liquid cellulase |
Non-Patent Citations (3)
Title |
---|
夏志兰等: "灵芝孢子粉生物酶破壁技术的研究", 《食用菌学报》 * |
张守勤等: "灵芝孢子食用方法与破壁技术研究进展", 《农业机械学报》 * |
谢永芳等: "一种新复合酶处理的灵芝孢子粉破壁研究", 《江苏农业科学》 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106867657A (en) * | 2017-03-17 | 2017-06-20 | 广州康琪莱生物科技有限公司 | A kind of efficient, multi-stage supercritical CO2The method of Ganoderma Lucidum Spores Oil Extracted |
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