CN104042520A - Water extraction and alcohol precipitation method of sarcandra glabra liquid extract - Google Patents

Abstract

The invention discloses a water extraction and alcohol precipitation method of a sarcandra glabra liquid extract and relates to the technical field of effective constituents of vegetable drugs. The water extraction and alcohol precipitation method comprises the following steps: A, putting the sarcandra glabra liquid extract obtained by virtue of water extraction into an alcohol precipitation tank, adding 90%-95% edible alcohol by 3-4 times volume and stirring evenly, standing for more than 24 hours and extracting the supernatant; B, putting the supernatant obtained in the step A into a vacuum concentrator for concentrating until the relative density is 1.25-1.26, thereby obtaining the sarcandra glabra liquid extract higher in effective component content. Compared with the prior art, the water extraction and alcohol precipitation method is capable of obtaining the sarcandra glabra liquid extract higher in effective component content by virtue of re-extraction by use of the edible alcohol.

Description

Sarcandra glabra fluid extract aqueous extraction-alcohol precipitation technology method

Technical field

The present invention relates to active ingredient of autonomic drug technical field, especially a kind of sarcandra glabra fluid extract aqueous extraction-alcohol precipitation technology method.

Background technology

Sarcandra glabra fluid extract has antibacterial, and anastalsis also has defying age, the loss of anti-ultraviolet, anti-keratin protein, and the multi-efficiencies such as skin protection, are a kind of desirable cosmetics of super quality additives.Its extracting method normally adopts hot water lixiviate, obtain extracting solution after reconcentration become sarcandra glabra fluid extract, this sarcandra glabra fluid extract made from water extraction is impure more, easily causes the effective ingredient isofraxidin of sarcandra glabra fluid extract to detect defective.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of sarcandra glabra fluid extract aqueous extraction-alcohol precipitation technology method, to solve the impure more problem of the sarcandra glabra fluid extract made from water extraction.

In order to address the above problem, the technical solution used in the present invention is: this sarcandra glabra fluid extract aqueous extraction-alcohol precipitation technology method, comprises the following steps:

A, the sarcandra glabra fluid extract made from water extraction is put into the heavy tank of wine, add 90%～95% edible ethanol of 3 times～4 times of volumes and stir, standing more than 24 hours, extract supernatant;

B, steps A gained supernatant is put into vacuum concentrator, and to be concentrated into relative density be 1.25～1.26 can obtain the sarcandra glabra fluid extract that active constituent content is higher.

Owing to having adopted technique scheme, the present invention compared with prior art has following beneficial effect:

The present invention puts into the heavy tank of wine by the sarcandra glabra fluid extract made from water extraction, add 90%～95% edible ethanol of 3 times～4 times of volumes and stir, standing more than 24 hours, extracting supernatant concentration to relative density is 1.25～1.26, by again extracting with edible ethanol, can obtain the sarcandra glabra fluid extract that active constituent content is higher, the effective ingredient isofraxidin of sarcandra glabra fluid extract brings up to 0.025%～0.03% from original 0.015%～0.019%, and it is qualified that the effective ingredient isofraxidin of sarcandra glabra fluid extract detects.

The specific embodiment

Below in conjunction with specific embodiment, the invention will be further described:

Embodiment 1

A sarcandra glabra fluid extract aqueous extraction-alcohol precipitation technology method, concrete steps are as follows:

A, the sarcandra glabra fluid extract made from water extraction is put into the heavy tank of wine, add 90% edible ethanol of 3 times of volumes and stir, standing more than 24 hours, extract supernatant;

B, steps A gained supernatant is put into vacuum concentrator, and to be concentrated into relative density be 1.25 can obtain the sarcandra glabra fluid extract that active constituent content is higher, simultaneously recovered alcohol.

Embodiment 2

A sarcandra glabra fluid extract aqueous extraction-alcohol precipitation technology method, concrete steps are as follows:

A, the sarcandra glabra fluid extract made from water extraction is put into the heavy tank of wine, add 95% edible ethanol of 4 times of volumes and stir, standing more than 24 hours, extract supernatant;

B, steps A gained supernatant is put into vacuum concentrator, and to be concentrated into relative density be 1.26 can obtain the sarcandra glabra fluid extract that active constituent content is higher, simultaneously recovered alcohol.

Claims (1)

1. a sarcandra glabra fluid extract aqueous extraction-alcohol precipitation technology method, is characterized in that comprising the following steps:

A, the sarcandra glabra fluid extract made from water extraction is put into the heavy tank of wine, add 90%～95% edible ethanol of 3 times～4 times of volumes and stir, standing more than 24 hours, extract supernatant;

B, steps A gained supernatant is put into vacuum concentrator, and to be concentrated into relative density be 1.25～1.26 can obtain the sarcandra glabra fluid extract that active constituent content is higher.