CN109091508A - A kind of ginkgo biloba p.e - Google Patents
A kind of ginkgo biloba p.e Download PDFInfo
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- CN109091508A CN109091508A CN201811260975.2A CN201811260975A CN109091508A CN 109091508 A CN109091508 A CN 109091508A CN 201811260975 A CN201811260975 A CN 201811260975A CN 109091508 A CN109091508 A CN 109091508A
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- extract
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- ethyl acetate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/16—Ginkgophyta, e.g. Ginkgoaceae (Ginkgo family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
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- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a kind of ginkgo biloba p.es, wherein, it is prepared by the following method: (1) crushing dry ginkgo leaf, alcohol extracting is carried out in the ratio that solid-liquid ratio is 1:5-1:20 with the ethanol solution of 20%-70%, twice, combined extract is simultaneously concentrated into density as 1.1-1.2 to refluxing extraction；Filtering, filtrate are concentrated to give crude extract；(2) it after the suitable water dispersion of the crude extract that step (1) obtains, after adjusting pH to 4-5 with acid, is extracted with ethyl acetate, obtains extract after ethyl acetate layer concentration；(3) extract that step (2) obtains is through macroporous resin adsorption, the 3-5 column volume of ethanol elution for being 95% with volume fraction, and eluent is concentrated, dry finished product.Compared with prior art, ginko leaves flavone content of the present invention is high, and extracting method is simple to operation, by the above method, can greatly save time cost, and extraction effect is more preferable, has extraordinary economic value and industrial value.
Description
Technical field
The present invention relates to a kind of ginkgo biloba p.es.
Background technique
The usual ginko leaves flavone content of ginkgo biloba extract is lower and complex for operation step in the prior art and extraction effect not
It is ideal.
Summary of the invention
In view of this, the technical problems to be solved by the present invention are: providing a kind of ginkgo leaf that ginko leaves flavone content is high
Extract.
In order to achieve the above object, the present invention adopts the following technical scheme that realize:
A kind of ginkgo biloba p.e, wherein it is prepared by the following method:
(1) dry ginkgo leaf is crushed, is carried out with the ethanol solution of 20%-70% in the ratio that solid-liquid ratio is 1:5-1:20
Alcohol extracting, twice, combined extract is simultaneously concentrated into density as 1 .1-1 .2 to refluxing extraction；Filtering, filtrate are concentrated to give crude extract；
(2) it after the suitable water dispersion of the crude extract that step (1) obtains, after adjusting pH to 4-5 with acid, is extracted with ethyl acetate, acetic acid second
Extract is obtained after ester layer concentration；(3) extract that step (2) obtains is through macroporous resin adsorption, the second for being 95% with volume fraction
Alcohol elutes 3-5 column volume, and eluent is concentrated, dry finished product.
Preferably, macroporous resin adsorption method is by macroreticular resin on concentrate, column volume 1L/kg in step (3)
Raw medicinal herbs is first eluted with water 6 times of volumes, then with 60% ethanol elution, 8 times of volumes and collects alcohol eluen, and eluent is concentrated into nothing
Alcohol is extracted with ethyl acetate in the ratio of 1:0 .2-1:2, and same method extraction three times, respectively obtains water phase and ester
Phase.
Preferably, model AB-8 or the D101 type of the macroreticular resin.
As shown from the above technical solution, the beneficial effects of the present invention are:
Compared with prior art, ginko leaves flavone content of the present invention is high, and extracting method is simple to operation, can by the above method
To greatly save time cost, and extraction effect is more preferable, has extraordinary economic value and industrial value.
Specific embodiment
In order to enable those skilled in the art to be further understood that feature and technology contents of the invention, please referring to following has
Close detailed description of the invention.
Embodiment 1:
A kind of ginkgo biloba p.e, wherein it is prepared by the following method:
(1) dry ginkgo leaf is crushed, carries out alcohol extracting, refluxing extraction in the ratio that solid-liquid ratio is 1:6 with 20% ethanol solution
Twice, combined extract and be concentrated into density be 1 .1；Filtering, filtrate are concentrated to give crude extract；(2) what step (1) obtained slightly mentions
It after the suitable water dispersion of object, after adjusting pH to 4-5 with acid, is extracted with ethyl acetate, obtains extract after ethyl acetate layer concentration；
(3) extract that step (2) obtains is through macroporous resin adsorption, 3 column volumes of ethanol elution for being 95% with volume fraction, elution
Liquid concentration, dry finished product.
Embodiment 2:
(1) dry ginkgo leaf is crushed, carries out alcohol extracting in the ratio that solid-liquid ratio is 1:7 with 30% ethanol solution, reflux mentions
It takes twice, combined extract is simultaneously concentrated into density as 1 .15；Filtering, filtrate are concentrated to give crude extract；(2) step (1) obtains
It after the suitable water dispersion of crude extract, after adjusting pH to 5 with acid, is extracted with ethyl acetate, obtains extract after ethyl acetate layer concentration；
(3) extract that step (2) obtains is through macroporous resin adsorption, 5 column volumes of ethanol elution for being 95% with volume fraction, elution
Liquid concentration, dry finished product.
Embodiment 3:
(1) dry ginkgo leaf is crushed, carries out alcohol extracting in the ratio that solid-liquid ratio is 1:17 with 50% ethanol solution, reflux mentions
It takes twice, combined extract is simultaneously concentrated into density as 1 .18；Filtering, filtrate are concentrated to give crude extract；(2) step (1) obtains
It after the suitable water dispersion of crude extract, after adjusting pH to 5 with acid, is extracted with ethyl acetate, obtains extract after ethyl acetate layer concentration；
(3) extract that step (2) obtains is through macroporous resin adsorption, 5 column volumes of ethanol elution for being 95% with volume fraction, elution
Liquid concentration, dry finished product.
Embodiment 4:
(1) dry ginkgo leaf is crushed, carries out alcohol extracting in the ratio that solid-liquid ratio is 1:10 with 40% ethanol solution, flowed back
It extracts twice, combined extract is simultaneously concentrated into density as 1 .2；Filtering, filtrate are concentrated to give crude extract；(2) step (1) obtains
It after the suitable water dispersion of crude extract, after adjusting pH to 4.5 with acid, is extracted with ethyl acetate, must be extracted after ethyl acetate layer concentration
Object；(3) extract that step (2) obtains is through macroporous resin adsorption, 3.8 cylinders of ethanol elution for being 95% with volume fraction
Product, by macroreticular resin on concentrate, column volume is 1L/kg raw medicinal herbs, is first eluted with water 6 times of volumes, then with 60% ethanol elution 8
Times volume simultaneously collects alcohol eluen, and eluent is concentrated into no alcohol, is extracted with ethyl acetate in the ratio of 1:2, same side
Method extracts three times, respectively obtains water phase and ester phase, eluent concentration, dry finished product.
Model AB-8 or the D101 type of the macroreticular resin.
Embodiment 5:
(1) dry ginkgo leaf is crushed, carries out alcohol extracting in the ratio that solid-liquid ratio is 1:15 with 60% ethanol solution, reflux mentions
It takes twice, combined extract is simultaneously concentrated into density as 1 .2；Filtering, filtrate are concentrated to give crude extract；(2) step (1) obtains thick
It after the suitable water dispersion of extract, after adjusting pH to 4.5 with acid, is extracted with ethyl acetate, obtains extract after ethyl acetate layer concentration；
(3) for the extract that step (2) obtains through macroporous resin adsorption, 3.8 column volumes of ethanol elution for being 95% with volume fraction will
Macroreticular resin on concentrate, column volume are 1L/kg raw medicinal herbs, are first eluted with water 6 times of volumes, then with 60% ethanol elution, 8 times of bodies
Alcohol eluen is accumulated and collects, eluent is concentrated into no alcohol, is extracted with ethyl acetate in the ratio of 1:2, same method extraction
It takes three times, respectively obtains water phase and ester phase, eluent concentration, dry finished product.
Model AB-8 or the D101 type of the macroreticular resin.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.In addition, it should be understood that although this specification is described in terms of embodiments, but it is not each
Embodiment only contains an independent technical solution, and this description of the specification is merely for the sake of clarity, this field
Technical staff should consider the specification as a whole, and the technical solutions in the various embodiments may also be suitably combined, form this
The other embodiments that field technical staff is understood that.
Claims (3)
1. a kind of ginkgo biloba p.e, which is characterized in that it is prepared by the following method:
(1) dry ginkgo leaf is crushed, is carried out with the ethanol solution of 20%-70% in the ratio that solid-liquid ratio is 1:5-1:20
Alcohol extracting, twice, combined extract is simultaneously concentrated into density as 1 .1-1 .2 to refluxing extraction；Filtering, filtrate are concentrated to give crude extract；
(2) it after the suitable water dispersion of the crude extract that step (1) obtains, after adjusting pH to 4-5 with acid, is extracted with ethyl acetate, acetic acid second
Extract is obtained after ester layer concentration；(3) extract that step (2) obtains is through macroporous resin adsorption, the second for being 95% with volume fraction
Alcohol elutes 3-5 column volume, and eluent is concentrated, dry finished product.
2. ginkgo biloba p.e according to claim 1, which is characterized in that macroporous resin adsorption method is in step (3)
By macroreticular resin on concentrate, column volume is 1L/kg raw medicinal herbs, 6 times of volumes is first eluted with water, then with 8 times of 60% ethanol elution
Volume simultaneously collects alcohol eluen, and eluent is concentrated into no alcohol, is extracted with ethyl acetate in the ratio of 1:0 .2-1:2, equally
Method extraction three times, respectively obtain water phase and ester phase.
3. ginkgo biloba p.e according to claim 2, which is characterized in that the model AB-8 of the macroreticular resin or
D101 type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811260975.2A CN109091508A (en) | 2018-10-26 | 2018-10-26 | A kind of ginkgo biloba p.e |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811260975.2A CN109091508A (en) | 2018-10-26 | 2018-10-26 | A kind of ginkgo biloba p.e |
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| Publication Number | Publication Date |
|---|---|
| CN109091508A true CN109091508A (en) | 2018-12-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201811260975.2A Pending CN109091508A (en) | 2018-10-26 | 2018-10-26 | A kind of ginkgo biloba p.e |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110917224A (en) * | 2019-06-17 | 2020-03-27 | 中国林业科学研究院林产化学工业研究所 | Method for extracting antioxidant from ginkgo leaf waste residues by adopting aqueous alkali |
-
2018
- 2018-10-26 CN CN201811260975.2A patent/CN109091508A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110917224A (en) * | 2019-06-17 | 2020-03-27 | 中国林业科学研究院林产化学工业研究所 | Method for extracting antioxidant from ginkgo leaf waste residues by adopting aqueous alkali |
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Application publication date: 20181228 |
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