CN103285053A - Preparation method of mangrove acanthus ilicifolius extract - Google Patents
Preparation method of mangrove acanthus ilicifolius extract Download PDFInfo
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- CN103285053A CN103285053A CN2013102676067A CN201310267606A CN103285053A CN 103285053 A CN103285053 A CN 103285053A CN 2013102676067 A CN2013102676067 A CN 2013102676067A CN 201310267606 A CN201310267606 A CN 201310267606A CN 103285053 A CN103285053 A CN 103285053A
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Abstract
The invention discloses a preparation method of mangrove acanthus ilicifolius extract. The preparation method comprises the following steps of: material pretreatment, refluxing extraction, ultrasonic reextraction, decolorization and concentration and the like, i.e., firstly adopting an alcohol-refluxing method for extraction, obtaining crude extract liquid I and residue of the acanthus ilicifolius, then treating the residue by an ultrasonic extraction method, obtaining crude extract liquid II of the acanthus ilicifolius, then carrying out decolorization and concentration on the crude extract liquid I and the crude extract liquid II of the acanthus ilicifolius and obtaining the acanthus ilicifolius extract. The preparation method disclosed by the invention has the characteristics of being simple, being high in extraction efficiency, low in extraction cost and easy in industry production.
Description
Technical field
The invention belongs to technical field of plant extraction, be specifically related to the mangrove acanthus preparation method of extract.
Background technology
Lao Shu le (Acanthus illcifolius L) is called wooden Lao Shu le, the Lao Shu le of Shui, Gen Tiao flower, is acanthaceous plant, is a kind of mangrove plant with medical value.It mainly is distributed in the torrid zone, Southeast Asia and subtropical zone countries such as India, Thailand, mainly is distributed in southern coastal areas such as Hainan, Guangdong, Fujian, Guangxi in China, by the sea with autumn plant association such as eggplant, Hai Lan.Lao Shu le is upright shrub, and is high 1～2 meter, the leaf keratin, and oval shape lanceolar, root and Herb all can be used as medicine, and it is lightly seasoned, cold in nature, has the effect of heat clearing and blood cooling, dispersing swelling and dissipating binds, relieving cough and asthma.Among the people in country in Southeast Asia such as India, Thailand and China, be used for the treatment of acute, chronic hepatitis, asthma, rheumatism, paralysis, snakebite, analgesia and antiinflammatory action are arranged.Modern pharmacological research find Lao Shu le have protect the liver, antioxidation, antitumor kill effects such as leishmania.For promoting the exploitation of Lao this medicine resource of Shu le, at present more and more wider to the research range of Lao Shu le, and Lao Shu le preparation method of extract is complicated mostly at present, the extraction cost height is unsuitable for commercial production.
Summary of the invention
At the defective that exists in the background technology, technical problem to be solved by this invention provides that a kind of extracting method is simple, extraction ratio is high, extraction cost is low, is easy to industrial mangrove acanthus preparation method of extract.
For addressing the above problem, the present invention is achieved through the following technical solutions:
The mangrove acanthus preparation method of extract may further comprise the steps:
1) pretreatment of raw material: get old Mus le plant herb, or at least a in root, stem and the leaf of old Mus le plant, clean, drain, put into baking oven then, dry under 30～55 ℃ temperature, crushing screening again, Mus le powder until old, standby;
2) reflux, extract: it is that 70～80% alcoholic solution carries out reflux, extract, 1～3 time that the Lao Shu le powder that step 1) is made adopts concentration, and each 0.5～3h filters, and merges each filtrate and gets crude extract I and residue I;
3) the residue I of gained and concentration are 70～80% alcoholic solution mixing ultrasonic extraction again: with step 2), under 60～100 ℃ temperature, supersound extraction 1～2 time, each time of extracting is 0.5～2h, filters, melt cinder is separated, merge each filtrate, get crude extract II and residue II, wherein, the residue II is abandoned, and the ratio of the quality of residue 1 and the volume of alcoholic solution is 1kg:8～12L;
4) decolouring: Lao Shu le crude extract I and crude extract II are fully mixed, get mixed liquor, add active carbon then, reflux decolour 15～30min gets Lao Shu le destaining solution, and the ratio of the quality of wherein said active carbon and the volume of mixed liquor is 1kg:350～500L;
5) concentrate: the distilling under reduced pressure of old Mus le destaining solution to doing, is namely got Mus le extract until old.
In the such scheme, preferably, the particle diameter of the Lao Shu le powder after pulverizing in the step 1) is less than 0.25mm.
Further, the ratio of the volume of the quality of Lao Shu le powder and alcoholic solution is 1kg:5～10L preferably, step 2).
In the such scheme, the temperature of distilling under reduced pressure is 60～65 ℃ in the step 5), and pressure is 7 * 10
4Pa.
Beneficial effect of the present invention is:
The present invention adopts ethanol refluxing process to extract earlier, get Lao Shu le crude extract I and residue, residue with gained passes through ultrasonic extraction then, get Lao Shu le crude extract II, twice Lao Shu le crude extract decoloured, concentrates, namely obtain Lao Shu le extract of the present invention, have that method is simple, extraction ratio is high, extraction cost is low, be easy to industrial characteristics.
The specific embodiment
Below in conjunction with specific embodiment the present invention is further explained explanation, but is not limited to these embodiment.
Embodiment 1
The mangrove acanthus preparation method of extract may further comprise the steps:
1) pretreatment of raw material: get Lao Shu le plant herb and clean, drain, put into baking oven then, dry under 30 ℃ temperature, crushing screening obtains particle diameter less than Lao Shu le powder of 0.25mm again, and is standby;
2) reflux, extract: it is that 70% alcoholic solution carries out reflux, extract, 2 times that the Lao Shu le powder that step 1) is made adopts concentration, each 1.5h, filter, merge each filtrate and get crude extract I and residue I, wherein the ratio of the volume of the quality of Lao Shu le powder and alcoholic solution is 1kg:10L;
3) the residue I of gained and concentration are 70% alcoholic solution mixing ultrasonic extraction again: with step 2), under 70 ℃ temperature, supersound extraction 1 time, the time of extracting is 2h, filter, melt cinder is separated, get crude extract II and residue II, wherein the residue II is abandoned, the ratio of the quality of residue 1 and the volume of alcoholic solution is 1kg:12L;
4) decolouring: Lao Shu le crude extract I and crude extract II are fully mixed, get mixed liquor, add active carbon then, reflux decolour 20min gets Lao Shu le destaining solution, and the ratio of the quality of wherein said active carbon and the volume of mixed liquor is 1kg:400L;
5) concentrate: be 60 ℃ with Lao Shu le destaining solution in temperature, pressure is 7 * 10
4Under the condition of Pa, distilling under reduced pressure namely gets Mus le extract until old to doing.
Embodiment 2
The mangrove acanthus preparation method of extract may further comprise the steps:
1) pretreatment of raw material: root and the leaf of getting Lao Shu le plant are cleaned, and drain, and put into baking oven then, and dry under 50 ℃ temperature, crushing screening obtains particle diameter less than Lao Shu le powder of 0.25mm again, and is standby;
2) reflux, extract: it is that 72% alcoholic solution carries out reflux, extract, 1 time that the Lao Shu le powder that step 1) is made adopts concentration, extraction time is 2h, filter, get crude extract I and residue I, wherein the ratio of the volume of the quality of Lao Shu le powder and alcoholic solution is 1kg:8L;
3) the residue I of gained and concentration are 72% alcoholic solution mixing ultrasonic extraction again: with step 2), under 80 ℃ temperature, supersound extraction 2 times, each time of extracting is 1.5h, filters, and melt cinder is separated, merge each filtrate, get crude extract II and residue II, wherein the residue II is abandoned, the ratio of the quality of residue 1 and the volume of alcoholic solution is 1kg:8～10L;
4) decolouring: Lao Shu le Lao Shu le crude extract I and crude extract II are fully mixed, get mixed liquor, add active carbon then, reflux decolour 25min gets Lao Shu le destaining solution, and the ratio of the quality of wherein said active carbon and the volume of mixed liquor is 1kg:480L;
5) concentrate: be that 62 ℃, pressure are 7 * 10 with Lao Shu le destaining solution in temperature
4Under the condition of Pa, distilling under reduced pressure namely gets Mus le extract until old to doing.
Embodiment 3
The mangrove acanthus preparation method of extract may further comprise the steps:
1) pretreatment of raw material: get the leaf of Lao Shu le plant, clean, drain, put into baking oven then, dry under 45 ℃ temperature, crushing screening obtains particle diameter less than Lao Shu le powder of 0.25mm again, and is standby;
2) reflux, extract: it is that 75% alcoholic solution carries out reflux, extract, 2 times that the Lao Shu le powder that step 1) is made adopts concentration, each 2h, filter, merge each filtrate and get crude extract I and residue I, wherein the ratio of the volume of the quality of Lao Shu le powder and alcoholic solution is 1kg:8L;
3) the residue I of gained and concentration are 75% alcoholic solution mixing ultrasonic extraction again: with step 2), under 85 ℃ temperature, supersound extraction 1 time, the time of extracting is 1.5h, filter, melt cinder is separated, get crude extract II and residue II, wherein the residue II is abandoned, the ratio of the quality of residue 1 and the volume of alcoholic solution is 1kg:10L;
4) decolouring: Lao Shu le crude extract I and crude extract II are fully mixed, get mixed liquor, add active carbon then, reflux decolour 28min gets Lao Shu le destaining solution, and the ratio of the quality of wherein said active carbon and the volume of mixed liquor is 1kg:500L;
5) concentrate: be 60～65 ℃ with Lao Shu le destaining solution in temperature, pressure is 7 * 10
4Under the condition of Pa, distilling under reduced pressure namely gets Mus le extract until old to doing.
Claims (4)
1. mangrove acanthus preparation method of extract is characterized in that may further comprise the steps:
1) pretreatment of raw material: get old Mus le plant herb, or at least a in root, stem and the leaf of old Mus le plant, clean, drain, put into baking oven then, dry under 30～55 ℃ temperature, crushing screening again, Mus le powder until old, standby;
2) reflux, extract: it is that 70～80% alcoholic solution carries out reflux, extract, 1～3 time that the Lao Shu le powder that step 1) is made adopts concentration, and each 0.5～3h filters, and merges each filtrate and gets crude extract I and residue I;
3) the residue I of gained and concentration are 70～80% alcoholic solution mixing ultrasonic extraction again: with step 2), under 60～100 ℃ temperature, supersound extraction 1～2 time, each time of extracting is 0.5～2h, filters, melt cinder is separated, merge each filtrate, get crude extract II and residue II, wherein, the residue II is abandoned, and the ratio of the quality of residue 1 and the volume of alcoholic solution is 1kg:8～12L;
4) decolouring: Lao Shu le crude extract I and crude extract II are fully mixed, get mixed liquor, add active carbon then, reflux decolour 15～30min gets Lao Shu le destaining solution, and the ratio of the quality of wherein said active carbon and the volume of mixed liquor is 1kg:350～500L;
5) concentrate: the distilling under reduced pressure of old Mus le destaining solution to doing, is namely got Mus le extract until old.
2. mangrove acanthus preparation method of extract according to claim 1 is characterized in that: the particle diameter of the Lao Shu le powder after pulverizing in the step 1) is less than 0.25mm.
3. mangrove acanthus preparation method of extract according to claim 1 is characterized in that: step 2) in the ratio of volume of the quality of Lao Shu le powder and alcoholic solution be 1kg:5～10L.
4. mangrove acanthus preparation method of extract according to claim 1, it is characterized in that: the temperature of distilling under reduced pressure is 60～65 ℃ in the step 5), pressure is 7 * 10
4Pa.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265793A (en) * | 2015-06-08 | 2017-01-04 | 深圳海王药业有限公司 | The application in preparation treatment senile dementia of the ocean mangrove mouse extract |
| CN107106622A (en) * | 2014-10-16 | 2017-08-29 | 同和药品株式会社 | The composition of extract or position containing justicia platymiscium |
| CN109105595A (en) * | 2018-10-18 | 2019-01-01 | 广东红树林生态科技有限公司 | A kind of preparation method of Lao Shu le root tea |
| WO2021114596A1 (en) * | 2019-12-12 | 2021-06-17 | 中国海洋大学 | Phenylethanoid glycoside extract from acanthus ilicifolius, and preparation method thereof and application as drug for resisting liver damage |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101306027A (en) * | 2008-07-09 | 2008-11-19 | 厦门大学 | Extracting method of medical mangrove acanthus total flavonoids |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101306027A (en) * | 2008-07-09 | 2008-11-19 | 厦门大学 | Extracting method of medical mangrove acanthus total flavonoids |
Non-Patent Citations (1)
| Title |
|---|
| 姚彤炜: "《药物分析》", 31 May 2011 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107106622A (en) * | 2014-10-16 | 2017-08-29 | 同和药品株式会社 | The composition of extract or position containing justicia platymiscium |
| CN106265793A (en) * | 2015-06-08 | 2017-01-04 | 深圳海王药业有限公司 | The application in preparation treatment senile dementia of the ocean mangrove mouse extract |
| CN106265793B (en) * | 2015-06-08 | 2019-10-29 | 深圳海王医药科技研究院有限公司 | Application of the ocean mangrove acanthus ilicifolius extract in preparation treatment senile dementia |
| CN109105595A (en) * | 2018-10-18 | 2019-01-01 | 广东红树林生态科技有限公司 | A kind of preparation method of Lao Shu le root tea |
| WO2021114596A1 (en) * | 2019-12-12 | 2021-06-17 | 中国海洋大学 | Phenylethanoid glycoside extract from acanthus ilicifolius, and preparation method thereof and application as drug for resisting liver damage |
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Application publication date: 20130911 |