CN101817809A - Method for producing flavonoids by using pectin-extracted orange peel - Google Patents

Method for producing flavonoids by using pectin-extracted orange peel Download PDF

Info

Publication number
CN101817809A
CN101817809A CN200910256055A CN200910256055A CN101817809A CN 101817809 A CN101817809 A CN 101817809A CN 200910256055 A CN200910256055 A CN 200910256055A CN 200910256055 A CN200910256055 A CN 200910256055A CN 101817809 A CN101817809 A CN 101817809A
Authority
CN
China
Prior art keywords
flavones
peel
pectin
alcohol
citrus reticulata
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN200910256055A
Other languages
Chinese (zh)
Inventor
张安明
胡晓燕
李霖善
房德
刘冰
杜启印
杨秀军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yantai DSM Andre Pectin Co Ltd
Original Assignee
Yantai Andre Pectin Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Yantai Andre Pectin Co Ltd filed Critical Yantai Andre Pectin Co Ltd
Priority to CN200910256055A priority Critical patent/CN101817809A/en
Publication of CN101817809A publication Critical patent/CN101817809A/en
Pending legal-status Critical Current

Links

Landscapes

  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The invention discloses a method for extracting, separating and purifying flavonoids from orange peel. In the method, the method for extracting an organic solvent is adopted and extraction is performed under the most optimal process conditions of extraction temperature of 90 DEG C, extraction time of 5 hours, 3 extraction times, 95 percent of alcohol concentration and a ratio of liquid to material of 1:30. The separation and purification adopt macroporous absorbent resin distributed elution to finally obtain orange flavonoids having purity of 90 percent. The method has the advantages of obtaining relatively higher yield and purity, utilizing industrial wastes, reducing environmental pollution and fulfilling the aim of resource comprehensive utilization.

Description

A kind of method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones of extracting of utilizing
Technical field:
The present invention relates to the extractive technique field of flavones, is a kind of method that flavones is produced in the peel of Citrus reticulata Blanco separation and purification behind the pectin of extracting of utilizing specifically.
Technical background:
Peel of Citrus reticulata Blanco has another name called dried orange peel, the TAO Hong-Jing meaning: " orange peel is good with old person of a specified duration " claims dried orange peel so practise.Traditional Chinese medicine thinks that dried orange peel has dispelling cold and eliminating phlegm, and cough-relieving is promoted the production of body fluid, and regulates the flow of vital energy to be good for the stomach, the logical effect of the temperature of loosing.Modern medicine study shows that the orange peel extract has anti-oxidant, and is anti-ageing, antitumor, and antianaphylaxis is antiviral, and anti-inflammatory prevents the cardiovascular disorder isoreactivity.What wherein mainly act in fact is flavonoid compound.Tangeritin is the natural green compound that extracts from peel of Citrus reticulata Blanco, and it is potential much to have immeasurable pharmacology to be worth to we mankind.According to research reports, the various physiological functions of tangeritin are as follows: (1) oxidation-resistance comprises that removing singly revolves oxygen, superoxide, hydroxyl radical free radical and other free radical etc.; (2) anticancer and antitumor action comprises that resistance is different, antiproliferative and anticancer invasion etc.; (3) prevention recycle system illness effect comprises strengthening capillary vessel toughness, suppresses platelet aggregation and regulates effects such as cardiovascular; (4) anti-inflammatory and anti-allergic effects; (5) anti-microbial effect comprises fungi and bacterium or the like.
It all is directly will press the dry peel of Citrus reticulata Blanco of orange juice as raw material that existing peel of Citrus reticulata Blanco extracts flavones, and contain a large amount of pectin in the peel of Citrus reticulata Blanco, as the pectin that is present in plant cell wall karyocyte internal layer, it is a kind of material that provides support for the inside plants cell, under existing extraction conditions, pectin can suppress the destruction of extracting solution to the inside plants cell, causes flavones extraction yield and purity all lower.
Summary of the invention:
The present invention has overcome the deficiency of above-mentioned prior art, and a kind of method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones of extracting of utilizing is provided, yield and the lower problem of purity when mainly solving existing method and extracting flavones.
In order to achieve the above object, the present invention is achieved in that a kind of the utilization extract the method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones, and its special character is that it comprises following processing step:
A raw material: the peel of Citrus reticulata Blanco that has extracted pectin in the industrial production;
B extracts: 95% the alcohol that adds 30 times of weight of peel of Citrus reticulata Blanco extracts, and 90 ℃ of temperature are extracted 5h, extract 3 times;
C filters: solid-liquid separation, remove waste residue, and keep extracting solution;
D purifying: extracting solution is adsorbed (AB-8) with macroporous resin;
The e dissolving: the alcohol with 80% will be adsorbed on the flavones dissolving on the resin column;
F concentrates: the spirituous solution of flavones is concentrated;
G alcohol reclaims: the alcohol in reclaiming extracting solution and concentrating;
H drying: the concentrated solution of flavones is carried out vacuum-drying, make the finished product.
Compared with the prior art a kind of method of utilizing the peel of Citrus reticulata Blanco that extracts behind the pectin to produce flavones of the present invention has outstanding substantive distinguishing features and marked improvement: adopt the extraction process of the flavones in the peel of Citrus reticulata Blanco behind the intact pectin of alcoholic extraction and the method for separation and purification, higher yield and purity have not only been obtained, make yield bring up to 3% by present 2%, purity brings up to 90% by present 70%, also industrial waste has been carried out utilization of waste material simultaneously, reduce environmental pollution, reached the purpose of comprehensive utilization of resources.
Embodiment:
In order to understand better and to implement, a kind of method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones of extracting of utilizing of the present invention is described below in conjunction with embodiment.
Embodiment 1, the peel of Citrus reticulata Blanco of pectin will have been extracted in the industrial production, 95% the alcohol that adds 30 times of weight of peel of Citrus reticulata Blanco extracts, 90 ℃ of temperature, extract 5h, extract 3 times, filter then, solid-liquid separation, remove waste residue, keep extracting solution, extracting solution is adsorbed with macroporous resin, the macroporous resin model is AB-8, to be adsorbed on flavones dissolving on the resin column with 80% alcohol again, the spirituous solution with flavones concentrates then, reclaim extracting solution and concentrate in alcohol, concentrated solution with flavones carries out vacuum-drying at last, makes the finished product.
After testing, the flavones yield is 3%, and purity is 90%.

Claims (1)

1. one kind is utilized and extracts the method that the peel of Citrus reticulata Blanco behind the pectin is produced flavones, it is characterized in that it comprises following processing step:
A raw material: the peel of Citrus reticulata Blanco that has extracted pectin in the industrial production;
B extracts: 95% the alcohol that adds 30 times of weight of peel of Citrus reticulata Blanco extracts, and 90 ℃ of temperature are extracted 5h, extract 3 times;
C filters: solid-liquid separation, remove waste residue, and keep extracting solution;
The d purifying: extracting solution is adsorbed with macroporous resin, and the macroporous resin model is AB-8;
The e dissolving: the alcohol with 80% will be adsorbed on the flavones dissolving on the resin column;
F concentrates: the spirituous solution of flavones is concentrated;
G alcohol reclaims: the alcohol in reclaiming extracting solution and concentrating;
H drying: the concentrated solution of flavones is carried out vacuum-drying, make the finished product.
CN200910256055A 2009-12-18 2009-12-18 Method for producing flavonoids by using pectin-extracted orange peel Pending CN101817809A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN200910256055A CN101817809A (en) 2009-12-18 2009-12-18 Method for producing flavonoids by using pectin-extracted orange peel

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN200910256055A CN101817809A (en) 2009-12-18 2009-12-18 Method for producing flavonoids by using pectin-extracted orange peel

Publications (1)

Publication Number Publication Date
CN101817809A true CN101817809A (en) 2010-09-01

Family

ID=42653105

Family Applications (1)

Application Number Title Priority Date Filing Date
CN200910256055A Pending CN101817809A (en) 2009-12-18 2009-12-18 Method for producing flavonoids by using pectin-extracted orange peel

Country Status (1)

Country Link
CN (1) CN101817809A (en)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102344429A (en) * 2011-07-22 2012-02-08 浙江省农业科学院 Method for extracting and purifying tangerine polymethoxyflavone
CN103263514A (en) * 2013-05-23 2013-08-28 浙江省柑桔研究所 Method for extracting flavones, low-molecule pectin and cellulose from orange peels in combined way
CN103304363A (en) * 2013-06-18 2013-09-18 浙江省柑桔研究所 Method for combined extraction of D-limonene, flavonoid and pectin from orange peel
CN103583936A (en) * 2013-10-23 2014-02-19 浙江蓝美农业有限公司 Blueberry jam added with citrus peel and preparation method thereof
CN104926767A (en) * 2014-03-18 2015-09-23 五邑大学 Method of purification preparation of citrus flavones by adopting macroporous adsorption resin
CN105663174A (en) * 2016-01-05 2016-06-15 安徽宇宁生物科技有限公司 Production technology for recovery of bioflavonoid from pectin extracted ethanol waste liquid
CN108129584A (en) * 2017-12-27 2018-06-08 中国农业科学院农产品加工研究所 It is a kind of to realize pectin and flavones while the method for extraction in citrus peel residue
CN111227247A (en) * 2020-03-10 2020-06-05 中国农业科学院农产品加工研究所 Citrus product with functions of improving intestinal flora structure and relieving atherosclerosis as well as preparation method and application of citrus product

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102344429A (en) * 2011-07-22 2012-02-08 浙江省农业科学院 Method for extracting and purifying tangerine polymethoxyflavone
CN102344429B (en) * 2011-07-22 2013-11-20 浙江省农业科学院 Method for extracting and purifying tangerine polymethoxyflavone
CN103263514A (en) * 2013-05-23 2013-08-28 浙江省柑桔研究所 Method for extracting flavones, low-molecule pectin and cellulose from orange peels in combined way
CN103304363A (en) * 2013-06-18 2013-09-18 浙江省柑桔研究所 Method for combined extraction of D-limonene, flavonoid and pectin from orange peel
CN103583936A (en) * 2013-10-23 2014-02-19 浙江蓝美农业有限公司 Blueberry jam added with citrus peel and preparation method thereof
CN103583936B (en) * 2013-10-23 2014-12-03 浙江蓝美农业有限公司 Blueberry jam added with citrus peel and preparation method thereof
CN104926767A (en) * 2014-03-18 2015-09-23 五邑大学 Method of purification preparation of citrus flavones by adopting macroporous adsorption resin
CN105663174A (en) * 2016-01-05 2016-06-15 安徽宇宁生物科技有限公司 Production technology for recovery of bioflavonoid from pectin extracted ethanol waste liquid
CN108129584A (en) * 2017-12-27 2018-06-08 中国农业科学院农产品加工研究所 It is a kind of to realize pectin and flavones while the method for extraction in citrus peel residue
CN111227247A (en) * 2020-03-10 2020-06-05 中国农业科学院农产品加工研究所 Citrus product with functions of improving intestinal flora structure and relieving atherosclerosis as well as preparation method and application of citrus product

Similar Documents

Publication Publication Date Title
CN101817809A (en) Method for producing flavonoids by using pectin-extracted orange peel
CN102993329B (en) Method for comprehensively extracting saponin, polysaccharides and polyphenol from camellia oleifera abel defatted cakes
CN102924240B (en) Method for extracting total magnolol according to alcoholic-alkaline method
CN101829164B (en) Biological preparation method of Hypericum perforatum L extractive
CN102603482B (en) Separation technology for multiple components in ginkgo leaf
CN101336949B (en) Method for extracting polysaccharide and flavone from Gynura divaricata
CN104523836A (en) Method for comprehensively extracting protein, polysaccharide and polyphenol from avocado kernels
CN101049328A (en) Technique for producing extractive of olive growing leaves
CN104840501A (en) Preparation method for total flavonoids of chrysanthemum
CN103387620A (en) Polysaccharide, total flavonoid and total alksloid prepared from lotus plumule and preparation method thereof
CN102993154A (en) Method for extracting purple sweet potato anthocyanin
CN103012544B (en) A kind of method extracting saponin and polysaccharide from tea seed grouts
CN101037467A (en) Method for separating purifying ursolic acid from loquat leaf
CN102477104A (en) Method for separating and purifying polysaccharide from Hovenia acerba
CN101503357A (en) Method for extracting chlorogenic acid in honeysuckle
CN102204950B (en) Method for extracting flavonoid from liquorice waste residue
CN106214838B (en) Method for recovering total flavone extract from plant polysaccharide water extract by using microchannel extraction device
CN107857825A (en) A kind of extracting method of radix tetrastigme polysaccharide
CN104558227A (en) Method for extracting ganoderan
CN103285053A (en) Preparation method of mangrove acanthus ilicifolius extract
CN103833805A (en) Process for refining glycyrrhizinic acid in liquorice
CN102643261A (en) Method for extracting cyanidin from blueberry wine residues
CN106317005B (en) Method for extracting rose flavone from rose dreg water
CN104586917A (en) Method for extracting total flavonoid from dragon fruit peels
CN101450972A (en) Ethanol concentration gradient technique for lily polysaccharide purification

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
ASS Succession or assignment of patent right

Owner name: YANTAI ANDRE PECTIC CO., LTD.

Free format text: FORMER OWNER: ANDELI PECTIN CO., LTD., YANTAI

Effective date: 20110603

C41 Transfer of patent application or patent right or utility model
TA01 Transfer of patent application right

Effective date of registration: 20110603

Address after: 264100 No. 889 Xincheng street, Muping District, Shandong, Yantai

Applicant after: Yantai Andre Pectin Co., Ltd.

Address before: 264100 No. 889 Xincheng street, Muping District, Shandong, Yantai

Applicant before: Andeli Pectin Co., Ltd., Yantai

C02 Deemed withdrawal of patent application after publication (patent law 2001)
WD01 Invention patent application deemed withdrawn after publication

Open date: 20100901