CN1884558B

CN1884558B - Method for intensively extracting red-rooted salvia polyphenol acids using composite enzyme hydrolyzing red-rooted salvia

Info

Publication number: CN1884558B
Application number: CN2006100139626A
Authority: CN
Inventors: 齐崴; 何志敏
Original assignee: Tianjin University
Current assignee: Tianjin University
Priority date: 2006-05-30
Filing date: 2006-05-30
Publication date: 2010-09-08
Anticipated expiration: 2026-05-30
Also published as: CN1884558A

Abstract

This invention provides the process of enzymatic enhanced extraction of depsides from salvia miltiorrhiza substance. This process includes comminution, soaking, the preparation of enzyme blends, the blending of material and liquid, extraction, filtering, condensing and dring. This process makes full use of effective wall breaking and improve dissolution principle of enzyme blends, and produces water-soluble extraction powder of salvia miltiorrhiza that with many depsides functional substance. This invention integrates the enzymatic hydrolysis and extraction process, and has many benefits suchas effective, mild, saving and environment friendly. The extraction rate of depsides from salvia miltiorrhiza is increased significently, reaches 9.5% that increased 3.5% compared with traditional water extraction. The temperature reduced from 100DEG C to 50DEG C, and time for extraction reduced for 1 hour. At the same time it avoids the Polyose Material Gelatinization and oxidation of Depsides such as danshensu. This process is innocuity and harmless. The products can be used as functional substance or medicine.

Description

The method of intensively extracting red-rooted salvia polyphenol acids using composite enzyme hydrolyzing red-rooted salvia

Technical field

The present invention relates to a kind of zymolysis technique that utilizes and strengthen the method for extracting salvia root polyphenol acids material, belong to the technology of obtaining natural product by biological enzyme.

Background technology

The red sage root has the effect of stasis-dispelling and pain-killing, promoting blood circulation to restore menstrual flow, is to treat coronary heart disease and anginal specific medicament clinically, and national annual total flow reaches hundred million tons.Kind surplus the salvia root polyphenol acids material of definite structure has ten, as: Salvianic acidA, coffic acid, rancinamycin IV, salvianolic acid (salvianolic acid) A, B, C, D, E, F, G, H, I, J, danshensuan B, third, rosmarinic acid (rosmarinic acid), alkannic acid (lithospermic acid) and different salvianolic acid C (isosalvianolic acid) etc., wherein Salvianic acidA is D-(+)-β-3,4-dihydroxy-benzene lactic acid is one of main water soluble component of the red sage root, also is that red sage root performance resists myocardial ischemia, the main functional component of pharmacological actions such as microcirculation improvement." FUFANG DANSHEN DIWAN of recording in the Chinese pharmacopoeia (version in 2005) promptly is to carry the potion name side that Salvianic acidA is a principal constituent with water.

In the various extraction processes of salvia root polyphenol acids material, comparatively traditional decocting method, the condensing reflux method, soxhlet extraction, all energy consumption is excessive, and supercritical carbon dioxide cyclic countercurrent extraction, the ultrasonic wave temperature control is extracted, the closed microwave auxiliary extraction, dynamic Continuous Countercurrent Extraction, methods such as capillary electrophoresis processing are ubiquity equipment requirements height again, solvent and energy consumption are big, many-sided problems such as processing power is low, and no matter adopt which kind of method, all target compound can not be extracted effectively fully, this is for more than one hundred million tons salvia miltiorrhiza raw material, be undoubtedly a kind of great waste, therefore pressing for design one cover, can to make effect good, cost is low, the salvia root polyphenol acids material that purity is high, and can increase substantially the novel process of extraction yield.

Summary of the invention

The object of the present invention is to provide a kind of method of intensively extracting red-rooted salvia polyphenol acids using composite enzyme hydrolyzing red-rooted salvia.This procedure is simple, and poly phenolic acid of Radix Salviae Miltiorrhizae purity height, the effect that makes is good, cost is low, can directly use as medicine.

For achieving the above object, the present invention adopts following technical proposals to be realized: earlier the red sage root being pulverized is 20～35 purpose particles, and be 1: 10～1: 20 by the ratio of red sage root quality g and water volume mL, it is that 0.1～0.2mol/L, pH are in acetic acid-sodium-acetate buffer of 4.6～5.6 that the red sage root is dipped in concentration; Be 0.01～0.05g/ml composite enzyme solution with the water-soluble concentration that is made into of two or more enzyme powder of cellulase, hemicellulase, zytase, polygalacturonase, amylase, lipase, proteolytic enzyme again; It is 1: 100～1: 300 by enzyme powder and red sage root mass ratio then, the adding of prozyme liquid is soaked with in the acetic acid-sodium acetate buffer solution of the red sage root, and fully stir, by heating in water bath maintenance system temperature is 30～50 ℃, by buffered soln maintenance system pH value 5～6, extraction time 60～100min; The gained extracting solution is the micro-filtrate membrane filtration slagging-off of 1～10 μ m with 1000～5000r/min centrifugation, 10～30min or via hole diameter; The gained filter residue carried out the secondary extracting of 20～40min again in 1: 5～1: 15 by solid-to-liquid ratio; Merge extracted twice liquid,, revolve inspissation with rotating speed 20～50r/min and contract in 40～50 ℃ of intervals of temperature; Concentrated solution is spray-dried again, its operational condition is: 150～200 ℃ of inlet temperatures, 70～85 ℃ of temperature outs, input speed 1.0～1.5L/h, working pressure 0.1～1MPa, or through lyophilize, its operational condition is: vacuum tightness＜133bar, temperature＜-40 ℃, promptly get salvia-soluble polyphenol acid extraction thing, comprising: Salvianic acidA, coffic acid, rancinamycin IV, salvianolic acid, rosmarinic acid, alkannic acid and the multiple depside effect of different salvianolic acid material.

The present invention integrates enzymolysis and extraction process, made full use of the enzymolysis broken wall, promote the principle of stripping, compare existing technology---multiple advantages such as traditional water decoction etc. has efficiently, gentleness, energy-saving and environmental protection: strengthened the extraction process process, make the extraction yield of salvia-soluble polyphenol acid higher by 3.55%, reduced production cost than traditional water formulation; The normal temperature and pressure operation, gentleness is easily controlled; Temperature is reduced to 50 ℃ from 100 ℃, has reduced energy consumption significantly, save energy; And do not have poisonous and harmful reagent contamination environment, belong to friendly process; In addition, extraction time shortens 1 hour than traditional technology, has alleviated equipment damage, has avoided the gelatinization of polysaccharose substance simultaneously, and having reduced also that polyphenol acids such as Salvianic acidA are oxidized in leaching process may.

Embodiment

For example the present invention is done below and describe in further detail.

Embodiment 1

Red sage root appropriateness is pulverized, and obtaining granularity is 30 purpose DANSHEN KELI; Take by weighing 100g, and by solid-to-liquid ratio 1 (g): 15 (ml), preparation 1500ml, 0.15mol/L, pH are acetic acid-sodium acetate buffer solution of 5.0 extraction agent as salvia root polyphenol acids material, and DANSHEN KELI is dipped in this extraction agent; It is 1: 200 by enzyme powder and DANSHEN KELI mass ratio again, preparation 10ml concentration be the prozyme liquid of 0.05g/ml as broken wall agent, wherein cellulase: hemicellulase: zytase: polygalacturonase: amylase: lipase: proteolytic enzyme=60: 25: 5: 5: 2: 2: 1 (mass ratio); Enzyme liquid and the buffered soln that is soaked with DANSHEN KELI are poured in the 3L extractor, 50 ℃ of constant temperature water baths, and continue to stir, the enzymolysis backflow integrated efficient that carries out 80min altogether extracts; Gained extracting solution via hole diameter is microfiltration membrane or the filter paper separating and filtering of 10 μ m, obtains the 130ml filtrate (extracting solution 1) and the 25g dregs of a decoction; By solid-to-liquid ratio 1 (g): 9 (ml), in the dregs of a decoction, add 225ml distilled water again, continue, separate obtaining 70ml filtrate (extracting solution 2) at 50 ℃ of following refluxing extraction 40min; Two portions extracting solution is merged, and at 45 ℃, rotating speed is to be concentrated into about 20ml under the condition of 30r/min in rotatory evaporator; Handle through lyophilize, condition is again: vacuum tightness 133bar, temperature-40 ℃; Finally make salvia-soluble polyphenol acid dry powder 9.53 g, extraction rate reached 9.53% is than the traditional water decoction acquisition of Duoing 3.55 gram target products.

Embodiment 2

Take by weighing and be crushed to 20 purpose DANSHEN KELI 600g, by solid-to-liquid ratio 1 (g): 20 (ml), preparation 12L, 0.1mol/L, pH are acetic acid-sodium acetate buffer solution of 5.4 extraction agent as salvia root polyphenol acids material, and DANSHEN KELI is dipped in this extraction agent; Be 1: 300 by enzyme and DANSHEN KELI mass ratio again, preparation 100ml concentration be the prozyme liquid of 0.02g/ml as broken wall agent, wherein cellulase: hemicellulase: zytase: polygalacturonase: amylase=50: 30: 15: 3: 2 (mass ratio); Enzyme liquid and the buffered soln that is soaked with DANSHEN KELI are poured in the 20L extractor, 40 ℃ of chuck constant temperature water baths, and continue to stir, carry out the extraction of 100min altogether; The gained extracting solution obtains the 1.2L supernatant liquor with 2000r/min centrifugation 20min; Directly carry out spraying drying, condition is: 180 ℃ of inlet temperatures, 80 ℃ of temperature outs, input speed 1.0L/h, working pressure 0.5MPa; Finally make salvia-soluble polyphenol acid dry powder 51.42g, extraction rate reached 8.57% is than the about 18 gram target products of the traditional water decoction acquisition of Duoing.

Embodiment 3

Used prozyme liquid broken wall agent prescription is: cellulase: hemicellulase: polygalacturonase=60: 35: 5 (mass ratio), all the other are with embodiment 2.

Claims

1. the method for an intensively extracting red-rooted salvia polyphenol acids using composite enzyme hydrolyzing red-rooted salvia is characterized in that comprising following process: the red sage root is pulverized, and obtaining granularity is 30 purpose DANSHEN KELI; Take by weighing 100g, and by solid-to-liquid ratio 1g: 15ml, preparation 1500ml, 0.15mol/L, pH are acetic acid-sodium acetate buffer solution of 5.0 extraction agent as salvia root polyphenol acids material, and DANSHEN KELI is dipped in this extraction agent; It is 1: 200 by enzyme powder and DANSHEN KELI mass ratio again, preparation 10ml concentration be the prozyme liquid of 0.05g/ml as broken wall agent, wherein cellulase: hemicellulase: zytase: polygalacturonase: amylase: lipase: proteolytic enzyme=60: 25: 5: 5: 2: 2: 1 (mass ratio); Enzyme liquid and the buffered soln that is soaked with DANSHEN KELI are poured in the 3L extractor, 50 ℃ of constant temperature water baths, and continue to stir, the enzymolysis backflow integrated efficient that carries out 80min altogether extracts; Gained extracting solution via hole diameter is microfiltration membrane or the filter paper separating and filtering of 10 μ m, obtains the 130ml filtrate 1 and the 25g dregs of a decoction; By solid-to-liquid ratio 1g: 9ml, in the dregs of a decoction, add 225ml distilled water again, continue, separate obtaining 70ml filtrate 2 at 50 ℃ of following refluxing extraction 40min; Two portions filtrate is merged, and at 45 ℃, rotating speed is to be concentrated into about 20ml under the condition of 30r/min in rotatory evaporator; Handle through lyophilize, condition is again: vacuum tightness 133bar, temperature-40 ℃; Finally make salvia-soluble polyphenol acid dry powder 9.53g.