CN101849977A - Method for extracting polyphenol from Mongolian oak leaves - Google Patents
Method for extracting polyphenol from Mongolian oak leaves Download PDFInfo
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- CN101849977A CN101849977A CN 201010184272 CN201010184272A CN101849977A CN 101849977 A CN101849977 A CN 101849977A CN 201010184272 CN201010184272 CN 201010184272 CN 201010184272 A CN201010184272 A CN 201010184272A CN 101849977 A CN101849977 A CN 101849977A
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- polyphenol
- mongolian oak
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Abstract
The invention relates to a method for extracting polyphenol from Mongolian oak leaves, which is characterized by comprising the following steps: carrying out ultrasonic extraction on the Mongolian oak leaves dried in the shade by using alcohol-contained aqueous solution and the filtering to obtain an extraction solution; carrying out decompression concentration on the extraction solution until the weight percent of residual alcohol in a concentrated solution is lower than 30 percent; adding an amount of water to the concentrated solution to obtain a water suspension; then extracting the water suspension by using an ester solvent; and carrying out decompression concentration on an ester solvent layer until dryness to obtain the crude product of the Mongolian oak polyphenol. The operation process has the main characteristics of simple operation, fluent process, recoverable used reagents, no pollution, less energy consumption, low production cost and higher yield and purity and is suitable for scale production.
Description
Affiliated technical field:
The present invention relates to the natural product chemistry field, particularly a kind of method of from Mongolian oak leaves, extracting polyphenol.
The main feature of this operating procedure is simple to operate, the technology smoothness, and the recyclable utilization of agents useful for same, pollution-free, less energy consumption, production cost is low, and yield and purity are higher, adapt to large-scale production.
Background technology:
Studies show that, human numerous disease such as tumor, cardiovascular and cerebrovascular vessel, aging, hypertension etc. are all closely related with free radical, free radical is the product of body intracellular metabolic, under the normal physiological situation, its generation and removing are in dynamic equilibrium, but under some pathologic condition that air, water pollution, food additive, pesticide, heavy metal, radiation etc. produce, can cause a large amount of accumulation of free radical, cause the oxidative damage of life macromolecule, thereby cause multiple disease.The Polyphenols antioxidant will demonstrate huge curative effect at aspects such as removing free radical, enhancing activities of antioxidant enzymes, reduction lipid peroxide contents and protection DNA.Therefore, Polyphenols polyphenoils confrontation human body all has practical significance and effect at aspects such as slow down aging, Antiradiation injury, beauty and skin care and diseases prevention and treatment.Owing to contain abundant polyphenol substance in the Mongolian oak leaves, have good oxidation resistance, the present invention considers above situation exactly, utilizes ultrasonic extraction to extract polyphenol substance from Mongolian oak leaves.
Summary of the invention:
The object of the present invention is to provide a kind of method of from Mongolian oak leaves, extracting polyphenol, it is characterized in that the Mongolian oak leaves that will dry in the shade carries out supersound extraction with containing alcoholic acid aqueous solution, filter extracting solution, extracting solution concentrating under reduced pressure mass percent of residual alcohol in the concentrated solution is lower than 30%, add an amount of water in the concentrated solution, be aqueous suspension, then aqueous suspension is extracted with ester solvent, the ester solvent layer is evaporated to dried, is the thick product of Quercus mongolica polyphenol.
The used ethanol volume fraction that contains alcoholic acid aqueous solution is 10%～50%, and used solid-liquid ratio is 1: 5～1: 30, and the supersound extraction time is 20～40min, extracts 15～40 ℃ of temperature.
Amount of water is 2～10 times of concentrated solution volume.
Used ester solvent is meant a kind of in ethyl acetate, methyl acetate, propyl acetate, the butyl acetate or their mixed solvent, and consumption is 1～4 times of aqueous suspension volume.
The temperature of extracting concentrating under reduced pressure in the Mongolian oak leaves polyphenol process is 50～70 ℃.
Advantage of the present invention is:
1. whole process flow is simple, and is easy to operate, and production cost is low, less energy consumption.
2. employing supersound extraction has improved the extraction efficiency of polyphenol greatly.
3. all recyclable utilization of agents useful for same, free from environmental pollution, adapt to scale, suitability for industrialized production.
Specific embodiments:
Below, the present invention will be further detailed with embodiment, but it is not limited to embodiment or similar example.
Embodiment:
The Mongolian oak leaves 200g that dries in the shade, the aqueous solution with solid-liquid ratio adding in 1: 10 ethanol volume fraction 28% places ultrasonic device, and 25 ℃ of supersound extraction temperature are extracted 30min.After extracting end, solid-liquid separation is evaporated to 1500 milliliters of filtrates 200 milliliters of residues then under 60 ℃ of conditions, add 200 milliliters of extractions of ethyl acetate, and Quercus mongolica leaf polyphenol yield is 0.42%, purity 19.01%.
Claims (8)
1. method of from Mongolian oak leaves, extracting polyphenol, it is characterized in that the Mongolian oak leaves that will dry in the shade carries out supersound extraction with containing alcoholic acid aqueous solution, filter extracting solution, extracting solution concentrating under reduced pressure mass percent of residual alcohol in the concentrated solution is lower than 30%, add an amount of water in the concentrated solution, be aqueous suspension, then aqueous suspension is extracted with ester solvent, the ester solvent layer is evaporated to dried, is the thick product of Quercus mongolica polyphenol.
2. according to the described method of extracting polyphenol from Mongolian oak leaves of claim 1, it is characterized in that: the used ethanol volume fraction that contains alcoholic acid aqueous solution is 10%～50% in the process of extraction Mongolian oak leaves polyphenol.
3. according to the described method of extracting polyphenol from Mongolian oak leaves of claim 1, it is characterized in that: used solid-liquid ratio is 1: 5～1: 30 in the process of extraction Mongolian oak leaves polyphenol.
4. according to the described method of extracting polyphenol from Mongolian oak leaves of claim 1, it is characterized in that: the supersound extraction time of extracting the Mongolian oak leaves polyphenol is 20～40min.
5. according to the described method of extracting polyphenol from Mongolian oak leaves of claim 1, it is characterized in that: the supersound extraction temperature of extracting the Mongolian oak leaves polyphenol is 15～40 ℃.
6. according to the described method of extracting polyphenol from Mongolian oak leaves of claim 1, it is characterized in that: amount of water is 2～10 times of concentrated solution volume.
7. according to the described method of from Mongolian oak leaves, extracting polyphenol of claim 1, it is characterized in that: used ester solvent is meant a kind of in ethyl acetate, methyl acetate, propyl acetate, the butyl acetate or their mixed solvent, and consumption is 1～4 times of aqueous suspension volume.
8. according to the described method of extracting polyphenol from Mongolian oak leaves of claim 1, it is characterized in that: the temperature of extracting concentrating under reduced pressure in the Mongolian oak leaves polyphenol process is 50～70 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201010184272 CN101849977A (en) | 2010-05-27 | 2010-05-27 | Method for extracting polyphenol from Mongolian oak leaves |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201010184272 CN101849977A (en) | 2010-05-27 | 2010-05-27 | Method for extracting polyphenol from Mongolian oak leaves |
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| CN101849977A true CN101849977A (en) | 2010-10-06 |
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| CN 201010184272 Pending CN101849977A (en) | 2010-05-27 | 2010-05-27 | Method for extracting polyphenol from Mongolian oak leaves |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104931446A (en) * | 2015-05-30 | 2015-09-23 | 北华大学 | Component extraction and detection method for material for brewing oak barrel |
| CN110731977A (en) * | 2019-11-28 | 2020-01-31 | 吉林省蚕业科学研究院 | Preparation method and application of oak bark extracts |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284022A (en) * | 2007-06-01 | 2008-10-15 | 山西省农业科学院农产品综合利用研究所 | Complexation extraction method of plant polyphenols substances |
-
2010
- 2010-05-27 CN CN 201010184272 patent/CN101849977A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101284022A (en) * | 2007-06-01 | 2008-10-15 | 山西省农业科学院农产品综合利用研究所 | Complexation extraction method of plant polyphenols substances |
Non-Patent Citations (2)
| Title |
|---|
| 《东北林业大学学报》 20100131 刘洋,等 蒙古栎叶片多酚的超声提取、优化及抗氧化能力 70-73 1-8 第38卷, 第1期 2 * |
| 《化工技术与开发》 20091031 张素霞 不同方法提取茶多酚的比较研究 28-30 1-8 第38卷, 第10期 2 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104931446A (en) * | 2015-05-30 | 2015-09-23 | 北华大学 | Component extraction and detection method for material for brewing oak barrel |
| CN110731977A (en) * | 2019-11-28 | 2020-01-31 | 吉林省蚕业科学研究院 | Preparation method and application of oak bark extracts |
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Open date: 20101006 |