CN101033175A - Method of extracting veratric alcohol from peanut plant after harvesting peanut fruit - Google Patents
Method of extracting veratric alcohol from peanut plant after harvesting peanut fruit Download PDFInfo
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- CN101033175A CN101033175A CNA2007100720657A CN200710072065A CN101033175A CN 101033175 A CN101033175 A CN 101033175A CN A2007100720657 A CNA2007100720657 A CN A2007100720657A CN 200710072065 A CN200710072065 A CN 200710072065A CN 101033175 A CN101033175 A CN 101033175A
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- resveratrol
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
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Abstract
This invention relates to a method for picking up resveratrol from the plants after harvesting peanut fruits, which cuts peanut plants into sections and mixes them with a solution containing alcohol in proportion and picks up them in a homogenizer then to be filtered to get a pick-up solution then to be decompressed and concentrated to that mass percentage of the residual alcohol in it is lower than 10%, then adds water into the concentrated solution, which becomes water mixed suspended solution then to be extracted with an ester solvent to be decompressed and concentrated till dried to get un-degreased abstraction material then to be extracted with a low polarity solvent and the residual solid substance is the resveratrol rough product.
Description
Affiliated technical field:
The present invention relates to the natural product chemistry field, particularly a kind of method of from the peanut rhizome, extracting trans-resveratrol.Relate to the homogenate of peanut rhizome and extract, extracting solution concentrating under reduced pressure, ester solvent extraction, processes such as low polar solvent degreasing.Be a kind of low cost, pollution-free, energy consumption is low, the product yield is high trans-resveratrol production technique.
The principal feature of this operating procedure is that raw material resources is abundant and be easy to get, and the technology smoothness is simple to operate, the recyclable utilization of agents useful for same, and less energy consumption, production cost is low, and yield is higher.
Background technology:
Trans-resveratrol is found in grape at first as a kind of important Polyphenols biochemical substances.Pharmacological research confirms, that trans-resveratrol has is antibiotic, anti-inflammatory, antianaphylaxis, anti thrombotic action; Has the inhibition platelet aggregation simultaneously, protection ischemic organ; Significantly anti-oxidant, anti-radical action (free radical is the cause that causes diseases such as aging, cancer, sacroiliitis); Preventive and therapeutic effect to coronary heart disease, hyperlipidemia; Anticancer, antimutagenic effect, thereby the generation of inhibition tumour.Past people all is to extract trans-resveratrol from giant knotweed, and giant knotweed is a kind of Chinese medicinal materials, and raw material resources is limited, and on the high side, is people's questions of common concern so develop the raw material of new extraction trans-resveratrol.After U.S. doctor Sanders announces " containing trans-resveratrol in the edible peanut ", caused in the world common concern to the peanut nourishing function.In recent years, many scholars measure the content of each position trans-resveratrol of peanut, and the approaches and methods that improves Resveratrol content has been carried out many research and development, and this is for the plant resources that enlarges trans-resveratrol, and meeting the need of market is significant.So the present invention considers above situation exactly, the technology of exploitation homogenate supersound extraction trans-resveratrol from the peanut rhizome.
Publication number is that the Chinese patent of CN1621401 has been introduced a kind of method of extracting high-purity resveratrol from giant knotweed, to add the certain amount of organic solvent refluxing extraction through the giant knotweed dry rhizome meal of pre-treatment, after reclaiming solvent, adopt the solvent of opposed polarity to carry out reflux operation.Owing to adopt refluxing extraction, so require extraction time longer, target component is heated and easily degrades or isomerization.
Publication number is the extraction process that the Chinese patent of CN1251361 has been introduced a kind of medicinal raw material trans-resveratrol, and it adopts, and the adding prozyme carries out enzyme digestion reaction acquisition in 48～72 hours enzymolysis raw material under constant temperature in Powdered giant knotweed raw material; Again with solvent extraction, the concentrated work in-process that obtain to contain trans-resveratrol, again through making with extra care promptly.Prozyme process operation parameter complexity, poor reproducibility, the industrialization difficulty is big.
Publication number is that the Chinese patent of CN1513822 has been introduced the technology of extracting trans-resveratrol from giant knotweed, it at first adopts the adding special microorganism to carry out bio-transformation raising giant knotweed Resveratrol content, utilize 40% ethanol and 4-methyl-2-amylalcohol mixture as extraction solvent, carry out microwave extraction.Though this method has improved product yield and content, it has used the mixture extraction, and solvent is reused proportioning and often is difficult to control, and 4-methyl-2-amylalcohol price height, causes whole technology cost higher.
Publication number is that the Chinese patent of CN1351985 has been introduced a kind of production technique of trans-resveratrol and the utilization in food, drink and grape wind thereof, this invention utilization is contained the Pericarpium Vitis viniferae of trans-resveratrol as raw material, through technologies such as sorting and impurity elimination, rinsing, fragmentation, 95% extraction using alcohols, produce the Pericarpium Vitis viniferae trans-resveratrol.Though this method yield is higher, starting material are also inadequate, and technology is complicated.
Publication number is the production method that the Chinese patent of CN1709846 has been introduced resveratrol extracted from peanut root, comprise raw materials pretreatment, with solvent extraction, high pressure chromatography wash-out, step such as refining, preprocessing process uses sodium hydroxide in a large number, and the waste water generation is big, difficult treatment; Alcohol immersion extraction time is long, and efficient is low, high pressure chromatography wash-out equipment requirements height, and operation easier is big.
Summary of the invention:
The object of the present invention is to provide a kind of method of from the peanut plant behind the results peanut fruit, extracting trans-resveratrol.The technical solution used in the present invention comprises: will gather in the crops after the peanut plant segment behind the peanut fruit to mix by certain solid-liquid ratio with aqueous solution of alcohol and put into refiner homogenate and extract, filter extracting solution, extracting solution concentrating under reduced pressure mass percent of residual alcohol in the concentrated solution is lower than 10%, add an amount of water in the concentrated solution, be aqueous suspension, then aqueous suspension is extracted with ester solvent, the ester solvent layer is evaporated to dried, get not degreasing extract, use not degreasing extract of low polar solvent extracting again, the thick product of the trans-resveratrol that remaining solid substance is.
The position of peanut plant comprises root, stem, leaf, shell.
Extract the used aqueous solution that contains alcohol in the process of trans-resveratrol and be the volume fraction that contains ethanol, methyl alcohol at 60%～95% the aqueous solution; The solid-liquid ratio that extracts trans-resveratrol is 1: 5～1: 30.
2000～10000 rev/mins of refiner revolutions, homogenate extraction time are 1～3 time, and the time that each homogenate is extracted is 3～6min.
Amount of water is 2～10 times of concentrated solution volume.
Extract used ester solvent in the process of trans-resveratrol and be meant a kind of in ethyl acetate, methyl acetate, propyl acetate, the butylacetate or their mixed solvent, consumption is 0.5～4 times of aqueous suspension volume.
The temperature of concentrating under reduced pressure is 30～70 ℃ in the process of extraction trans-resveratrol.
Low polar solvent is a kind of in sherwood oil, normal hexane, industrial naptha, the hexanaphthene or their mixed solvent.
Low polar solvent extracting number of times is 1～5 time, and the solid-to-liquid ratio of degreasing extract and low polar solvent is not 1: 1～1: 15.
Advantage of the present invention is:
1. whole process flow is simple, and is easy to operate, and production cost is low, less energy consumption.
2. adopt homogenate to extract, the extraction efficiency height, products obtained therefrom purity is higher.
3. all recyclable utilization of agents useful for same, free from environmental pollution, be easy to industrialization.
Specific embodiments:
Below, the present invention will be further detailed with embodiment, but it is not limited to any or the similar example of these embodiment.
Embodiment 1
Get 50g peanut rhizome, extract 3 times, each 4min with 90% ethanolic soln 600ml homogenate, filter, extracting solution is evaporated to the about 50ml of concentrated solution volume for 40 ℃, adds 50ml distilled water mixing in concentrated solution, uses the 100ml ethyl acetate extraction more respectively 3 times, the combined ethyl acetate phase is evaporated to dried for 45 ℃, use 100ml normal hexane extracting 3 times more respectively, filter, the filter cake drying under reduced pressure gets the trans-resveratrol crude product, the product yield is 92.6%, and purity is 2.66%.
Embodiment 2
Get 500g peanut rhizome, extract 3 times, each 6min with 90% methanol solution 5000ml homogenate, filter, 40 ℃ of concentrating under reduced pressure of extracting solution are until the about 400ml of concentrated solution volume, in concentrated solution, add 500ml distilled water mixing, again with 500ml methyl acetate extraction 2 times, merge methyl acetate mutually 45 ℃ be evaporated to driedly, use 400ml sherwood oil extracting 4 times more respectively, filter, the filter cake drying under reduced pressure gets the trans-resveratrol crude product, and the product yield is 93.8%, and purity is 3.02%.
Claims (10)
1. method of from the peanut plant behind the results peanut fruit, extracting trans-resveratrol, put into refiner homogenate extraction with mixing by certain solid-liquid ratio with aqueous solution of alcohol after the peanut plant segment behind the results peanut fruit, filter extracting solution, extracting solution concentrating under reduced pressure mass percent of residual alcohol in the concentrated solution is lower than 10%, add an amount of water in the concentrated solution, be aqueous suspension, then aqueous suspension is extracted with ester solvent, the ester solvent layer is evaporated to dried, get not degreasing extract, use not degreasing extract of low polar solvent extracting again, the trans-resveratrol phase product that remaining solid substance is.
2. according to the described method of extracting trans-resveratrol from the peanut plant behind the results peanut fruit of claim 1, it is characterized in that: the position of peanut plant comprises root, stem, leaf, shell.
3. according to the described method of from the peanut rhizome, extracting trans-resveratrol of claim 1, it is characterized in that: extract the used aqueous solution that contains alcohol in the process of trans-resveratrol and be the volume fraction that contains ethanol, methyl alcohol at 60%～95% the aqueous solution.
4. according to the described method of extracting trans-resveratrol from the peanut plant behind the results peanut fruit of claim 1, it is characterized in that: the solid-liquid ratio that extracts trans-resveratrol is 1: 5～1: 30.
5. according to the described method of extracting trans-resveratrol from the peanut plant behind the results peanut fruit of claim 1, it is characterized in that: 2000～10000 rev/mins of refiner revolutions, homogenate extraction time are 1～3 time, and the time that each homogenate is extracted is 3～6min.
6. according to the described method of extracting trans-resveratrol from the peanut plant behind the results peanut fruit of claim 1, it is characterized in that: amount of water is 2～10 times of concentrated solution volume.
7. according to the described method of from the peanut plant behind the results peanut fruit, extracting trans-resveratrol of claim 1, it is characterized in that: extract used ester solvent in the process of trans-resveratrol and be meant a kind of in ethyl acetate, methyl acetate, propyl acetate, the butylacetate or their mixed solvent, consumption is 0.5～4 times of aqueous suspension volume.
8. according to the described method of extracting trans-resveratrol from the peanut plant behind the results peanut fruit of claim 1, it is characterized in that: the temperature of concentrating under reduced pressure is 30～70 ℃ in the process of extraction trans-resveratrol.
9. according to the described method of extracting trans-resveratrol from the peanut rhizome of claim 1, it is characterized in that: low polar solvent is a kind of in sherwood oil, normal hexane, industrial naptha, the hexanaphthene or their mixed solvent.
10. according to the described method of extracting trans-resveratrol from the peanut plant behind the results peanut fruit of claim 1, it is characterized in that: low polar solvent extracting number of times is 1～5 time, and the solid-to-liquid ratio of degreasing extract and low polar solvent is not 1: 1～1: 15.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100720657A CN101033175A (en) | 2007-04-20 | 2007-04-20 | Method of extracting veratric alcohol from peanut plant after harvesting peanut fruit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100720657A CN101033175A (en) | 2007-04-20 | 2007-04-20 | Method of extracting veratric alcohol from peanut plant after harvesting peanut fruit |
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| CN101033175A true CN101033175A (en) | 2007-09-12 |
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| CNA2007100720657A Pending CN101033175A (en) | 2007-04-20 | 2007-04-20 | Method of extracting veratric alcohol from peanut plant after harvesting peanut fruit |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102994323A (en) * | 2011-09-09 | 2013-03-27 | 冯武文 | Method for improving content of resveratrol in wine base |
| CN103172498A (en) * | 2011-12-26 | 2013-06-26 | 山东省花生研究所 | Peanut resveratrol extraction method |
| CN104003849A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Extraction method of resveratrol from peanut buds |
| CN109078189A (en) * | 2018-10-23 | 2018-12-25 | 黄泳华 | Composition containing kinases inhibitor and resveratrol |
| CN110198726A (en) * | 2016-11-28 | 2019-09-03 | 韩国科学技术研究院 | It include peanut overground part extract and the composition of fraction and application thereof for treat dermatitis |
| CN113786426A (en) * | 2021-09-18 | 2021-12-14 | 山东省花生研究所 | Method for enriching flavonoid, terpenoid and/or stilbene compounds in peanut plants |
-
2007
- 2007-04-20 CN CNA2007100720657A patent/CN101033175A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102994323A (en) * | 2011-09-09 | 2013-03-27 | 冯武文 | Method for improving content of resveratrol in wine base |
| CN103172498A (en) * | 2011-12-26 | 2013-06-26 | 山东省花生研究所 | Peanut resveratrol extraction method |
| CN104003849A (en) * | 2013-09-17 | 2014-08-27 | 黄桂淑 | Extraction method of resveratrol from peanut buds |
| CN104003849B (en) * | 2013-09-17 | 2016-05-18 | 黄桂淑 | The extracting method of resveratrol in peanut sprout |
| CN110198726A (en) * | 2016-11-28 | 2019-09-03 | 韩国科学技术研究院 | It include peanut overground part extract and the composition of fraction and application thereof for treat dermatitis |
| CN109078189A (en) * | 2018-10-23 | 2018-12-25 | 黄泳华 | Composition containing kinases inhibitor and resveratrol |
| CN113786426A (en) * | 2021-09-18 | 2021-12-14 | 山东省花生研究所 | Method for enriching flavonoid, terpenoid and/or stilbene compounds in peanut plants |
| CN113786426B (en) * | 2021-09-18 | 2022-07-08 | 山东省花生研究所 | Method for enriching flavonoid, terpenoid and/or stilbene compounds in peanut plants |
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Open date: 20070912 |