CN104312939A - Selenite cystine enrichment broth and application thereof - Google Patents
Selenite cystine enrichment broth and application thereof Download PDFInfo
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- CN104312939A CN104312939A CN201410491384.1A CN201410491384A CN104312939A CN 104312939 A CN104312939 A CN 104312939A CN 201410491384 A CN201410491384 A CN 201410491384A CN 104312939 A CN104312939 A CN 104312939A
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- sodium
- enrichment broth
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- selenite
- hydrogen phosphate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention belongs to the microbial field, and especially relates to a selenite cystine enrichment broth. The culture medium consists of the components: 21 g/L of a gram negative enrichment broth, 4.0 g/L of lactose, 5.0 g/L of sodium hydrogen selenite, 7.55 g/L of disodium hydrogen phosphate, 4.5 g/L of L-cystine, 4.5 g/L of sodium malonate, and 2.9 g/L of sodium dihydrogen phosphate, wherein the pH value is 7.0-7.2. The gram negative enrichment broth contains 20.0 g of tryptone, 2.0 g of glucose, 1.5 g of dipotassium hydrogen phosphate, 3 g of mannitol, 5.8 g of sodium citrate, 0.33 g of deoxysodium cholate, 5.0 g of sodium chloride, and 2.0 g of dipotassium hydrogen phosphate in each liter of the gram negative enrichment broth. The selenite cystine enrichment broth has the advantages of simple culture medium formula, convenient operation, low cost, and favorable popularization and application; bacteria grow rapidly, and the mutation rate is low; and observation and analysis are convenient.
Description
Technical field
The present invention is microorganism field, particularly a kind of selenite cystine broth and application thereof.
Background technology
The pathogenic agent of salmonellosis.Belong to enterobacteriaceae, Gram-negative enteric bacillus.Nearly 1,000 kinds (or bacterial strains) of having found.By its antigenic component, can Salmonellas be divided into first, second, third, fourth, the basic bacterium group such as penta.Wherein relevant with the human body diseases paratyphoid A bacillus mainly containing first group, the paratyphoid B bacillus of second group and bacillus typhi murium, the paratyphoid C bacillus of third group and hog cholera bacillus, the Corynebacterium diphtheriae of fourth group and bacillus enteritidis etc.Except Corynebacterium diphtheriae, paratyphoid A bacillus and paratyphoid B bacillus cause Human diseases, great majority only can cause the disease of the animals such as domestic animal, muroid and bird by order, but sometimes also can pollute the food of the mankind and cause food poisoning.Salmonellas not easily breeds in water, but can survive 2-3 week, can survive 3-4 month in refrigerator, can survive 1-2 month in the ight soil of physical environment.The suitableeest the bread worm of Salmonellas is 37 DEG C, can amount reproduction more than 20 DEG C, and therefore, low-temperature storage food is important preventive measures.
Shigella is a class gram negative bacillus, is the most common pathogenic bacteria of human bacterial's property dysentery, common name dysentery bacterium.Size is 0.5 ~ 0.7 × 2 ~ 3 μm, without gemma, without pod membrane, and atrichia.Majority has pili.Gram negative bacillus.For facultative anaerobe, can grow on ordinary culture medium, form median size, translucent smooth colony.Intestinal bacilli selective medium forms colourless bacterium colony.Biochemical reaction decomposition glucose, produces acid not aerogenesis.VP negative, not decomposing urea, do not form hydrogen sulfide, and citrate can not be utilized as carbon source.Bacillus ceylonensis A energy delayed reaction lactose (37 DEG C 3 ~ 4 days).
Substratum (Medium) is for microorganism, plant and animal tissue growth and the nutriment of artificial preparation that maintains, generally all contains carbohydrate, nitrogenous substances, inorganic salt (comprising trace element) and VITAMIN and water etc.Some substratum also contain antibiotic and pigment, for single microorganism culturing and qualification.Substratum is due to the raw material difference of preparation, and service requirements is different, and storage and custody aspect is also slightly different.General substratum, being heated, after the moisture absorption, being easily contaminated by bacterial or decomposing rotten, therefore general substratum must protection against the tide, lucifuge, the preservation of shady and cool place.Some are needed to the substratum (as tissue culture medium (TCM)) of stringent sterilization, the storage of long period, must be placed in the refrigerator of 3 ~ 6 DEG C.Because liquid nutrient medium is not easily taken care of for a long time, all change system into powder now.
Substratum is natural medium according to chemical classification, combines substratum and partly combine substratum; Be liquid nutrient medium, film solid media and dehydrated medium according to physical classification; Be selective medium, differential medium according to microorganism classification.
Summary of the invention
The present invention overcomes deficiency of the prior art, provides a kind of selenite cystine broth and application thereof.
The present invention uses biological chemistry, the principle of microbiology and inorganic, organic, analytical chemistry and fluorescence technique, row filter is combined into all class nutrition compositions such as different carbon sources, nitrogenous source, inorganic salts, VITAMIN, growth stimulants, the materialization factor such as pH value, ionic strength, oxidation-reduction potential, surface tension, atmosphere surrounding of substratum is compared, investigated substratum of the present invention.
The technical solution adopted in the present invention is:
A kind of selenite cystine broth, the component of described substratum comprises 21g/LGN enrichment liquid, 4.0g/L lactose, 5.0g/L sodium hydroselenite, 7.55g/L Sodium phosphate dibasic, 4.5g/L CYSTINE, 4.5g/L sodium malonate, 2.9g/L SODIUM PHOSPHATE, MONOBASIC, and pH value is 7.0-7.2.
On the basis of above scheme, described GN enrichment liquid is containing Tryptones 20.0g, glucose 2.0g, dipotassium hydrogen phosphate 1.5g, N.F,USP MANNITOL 3g, Sodium Citrate 5.8g, sodium deoxycholate 0.33g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 2.0g in often liter of GN enrichment liquid.
The invention also discloses the application of described selenite cystine broth, for breeding the Salmonellas in sample.
Peptone provides Carbon and nitrogen sources to meet the demand of bacterial growth; Lactose is fermentable carbohydrate; Sodium hydroselenite suppresses most of Gram-negative enteric bacterium of gram-positive microorganism and nonsalmonella; Phosphoric acid salt is buffer reagent; CYSTINE is reductive agent.
Take this product 28g, heating for dissolving, in 1000ml distilled water, is waited to be chilled to 60 DEG C, impouring sterilized petri dishes.Without the need to autoclaving.
Beneficial effect of the present invention:
1, substratum prescription is simple, and preparation manipulation is convenient, and cost is low, is beneficial to and applies;
2, bacteria growing is rapid, and aberration rate is low;
3, be convenient to observe, analyze.
Embodiment
Embodiment 1:
A kind of selenite cystine broth, the component of described substratum comprises 21g/LGN enrichment liquid, 4.0g/L lactose, 5.0g/L sodium hydroselenite, 7.55g/L Sodium phosphate dibasic, 4.5g/L CYSTINE, 4.5g/L sodium malonate, 2.9g/L SODIUM PHOSPHATE, MONOBASIC, and pH value is 7.0-7.2.
Described GN enrichment liquid is containing Tryptones 20.0g, glucose 2.0g, dipotassium hydrogen phosphate 1.5g, N.F,USP MANNITOL 3g, Sodium Citrate 5.8g, sodium deoxycholate 0.33g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 2.0g in often liter of GN enrichment liquid.
Peptone provides Carbon and nitrogen sources to meet the demand of bacterial growth; Lactose is fermentable carbohydrate; Sodium hydroselenite suppresses most of Gram-negative enteric bacterium of gram-positive microorganism and nonsalmonella; Phosphoric acid salt is buffer reagent; CYSTINE is reductive agent.
Take this product 28g, heating for dissolving, in 1000ml distilled water, is waited to be chilled to 60 DEG C, impouring sterilized petri dishes.Without the need to autoclaving.
18-24 hour is cultivated at 36 ± 1 DEG C
| Quality-control strains | Growing state |
| Singly increase listeria spp | - |
| Streptococcus aureus | - |
| Intestinal bacteria | -/+ |
| Salmonellas | (10-8)+++ |
Claims (3)
1. a selenite cystine broth, it is characterized in that the component of described substratum comprises 21g/LGN enrichment liquid, 4.0g/L lactose, 5.0g/L sodium hydroselenite, 7.55g/L Sodium phosphate dibasic, 4.5g/L CYSTINE, 4.5g/L sodium malonate, 2.9g/L SODIUM PHOSPHATE, MONOBASIC, pH value is 7.0-7.2.
2. selenite cystine broth according to claim 1, is characterized in that described GN enrichment liquid is for containing Tryptones 20.0g, glucose 2.0g, dipotassium hydrogen phosphate 1.5g, N.F,USP MANNITOL 3g, Sodium Citrate 5.8g, sodium deoxycholate 0.33g, sodium-chlor 5.0g, dipotassium hydrogen phosphate 2.0g in often liter of GN enrichment liquid.
3. an application for selenite cystine broth according to claim 1, is characterized in that the Salmonellas for breeding in sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410491384.1A CN104312939A (en) | 2014-09-23 | 2014-09-23 | Selenite cystine enrichment broth and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410491384.1A CN104312939A (en) | 2014-09-23 | 2014-09-23 | Selenite cystine enrichment broth and application thereof |
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| Publication Number | Publication Date |
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| CN104312939A true CN104312939A (en) | 2015-01-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201410491384.1A Pending CN104312939A (en) | 2014-09-23 | 2014-09-23 | Selenite cystine enrichment broth and application thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703571A (en) * | 2011-06-29 | 2012-10-03 | 安徽出入境检验检疫局检验检疫技术中心 | Method for detecting salmonellas in dehydrated garlic products |
| CN104651449A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Selenite-cystine enrichment broth and use thereof |
-
2014
- 2014-09-23 CN CN201410491384.1A patent/CN104312939A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102703571A (en) * | 2011-06-29 | 2012-10-03 | 安徽出入境检验检疫局检验检疫技术中心 | Method for detecting salmonellas in dehydrated garlic products |
| CN104651449A (en) * | 2013-11-25 | 2015-05-27 | 刘汉斌 | Selenite-cystine enrichment broth and use thereof |
Non-Patent Citations (1)
| Title |
|---|
| 莫嘉延,等: "几种亚硒酸盐胱氨酸增菌液选择性增菌效果的比较", 《中国卫生检验杂志》 * |
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Application publication date: 20150128 |
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