CN104286421A - Medicament additive for preventing caecum hepatitis of chickens - Google Patents

Abstract

The invention provides a medicament additive for preventing caecum hepatitis of chickens. The medicament additive comprises the following raw material medicines of heartleaf houttuynia herbs, honeysuckle, gordon euryale seeds, Chinese yams, fineleaf schizonepeta herbs, small centipeda herbs, artemisiae scopariae, white peony roots, philippine violet herbs, Chinese thorowax, Chinese pulsatilla roots, gaultheria, stellaria uliginosa, goose grass, aurantii fructus immaturus, folium sennae, rhizoma corydalis, cape jasmine fruits, fine silene roots and rhizoma gastrodiae. The medicament additive is mixed into a feed, so that the medicament additive has a remarkable effect of preventing the caecum hepatitis of the chickens.

Description

For preventing the medicament additive of chicken blackhead

Technical field

The present invention relates to field of feed, particularly relating to a kind of for preventing the medicament additive of chicken blackhead.

Background technology

Chicken blackhead is a kind of protozoosis caused by the Histomanas Meleagridis of Histomonas, also known as histomoniasis or blackhead disease.Suffering from chicken principal pathogenetic feature is that caecum inflammation and liver surface have ulcer stove.This disease is often sent out in chick, and become chicken also can infect, general summer is multiple, and open chicken house chicken more easily infects this disease, and as treated not in time, its M & M will obviously rise.Recover chicken poor growth, egg production declines.

Suffer from chicken lassitude, chilly/subtract food, feather pine is random/matt.Have loose bowels, first passage of watery stools, arrange successively subsequently bloody stool/yellowish-white colour band bloody stool/yellowish-brown just/sulphur look just/black paste ight soil, its excrement stench.Suffer from cock skin skin cyanosis, it is blunt to show, and slow-witted on one side vertical, two wings are sagging, and what have is curved in wing, in stilt walk sample gait during walking.Phase suffers from chicken stomach inanition after being ill, and day by day become thin, health is rolled up, and what have can touch allantoid caecum at belly.Have spasm before short dead of the course of disease or performance of struggling, temperature decline before long dead of the course of disease, does not rise sleepingly.This sufferer chicken with desulphurization sulphur look ight soil and stilt walk sample gait for feature.

Usually after infection about 17 days, the death rate peaks, and then within the 28th day, starts to decline, and the chicken incidence of disease is 89%, and the death rate is 70%.

Chinese medicine comes from the Nature animal, plant, mineral matter and other products, is the material useful to humans and animals that mankind's long-term practice screens.Chinese medicine comes from the Nature animal, plant, mineral matter and other products, is the material useful to humans and animals that mankind's long-term practice screens.

Chinese veterinarian thinks chicken asthenic susceptibility of spleen, honey stomach, and digestive function is poor in addition, easily suffer from the disease such as enteritis, diarrhoea, and liver often has She, and heat-transmission in being easy to after ill to pass, often causes liver to move, easily suffer from chicken blackhead.Other internal organs are ill also often causes mixed infection, harm liver, intestines, spleen, each internal organs of stomach.According to differentiation of disease according to pathological changes of the viscera and their analysis, duck experiences virus attack liver, causes deficiency of heart-QI, and can not preside over blood circulation and to circulate each warp, enriching yin whole body, the stasis of blood amasss heat-dissipating, and blood-head is then withered, and of a specified duration then causes death.Therefore, treatment on clearing heat and promoting diuresis, removing toxic substances, soothing the liver spasmolysis, replenish qi to invigorate the spleen, promoting blood circulation and removing blood stasis for principle.The present invention is exactly in this theoretical foundation, proposition a kind of for preventing and treating the medicament of chicken blackhead and comprising the feed of this medicament, and it is Be very effective in control chicken blackhead.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind ofly to be admixed in feed for preventing the medicament additive of chicken blackhead, Be very effective in prevention chicken blackhead.

For solving the problems of the technologies described above, the invention provides a kind of for preventing the medicament additive of chicken blackhead, its bulk drug comprises cordate houttuynia, honeysuckle, Gorgon fruit, Chinese yam, schizonepeta, Centipeda minima, oriental wormwood, the root of herbaceous peony, Chinese violet, radix bupleuri, the Chinese bulbul, Gaultheria, bog starwort herb, eleusine indica, the dried immature fruit of citron orange, folium sennae, corydalis tuber, cape jasmine, Silence tenuis and rhizoma Gastrodiae.

Wherein, in described medicament additive, the weight of each bulk drug is respectively cordate houttuynia 22g ~ 32g, honeysuckle 43g ~ 53g, Gorgon fruit 17g ~ 27g, Chinese yam 26g ~ 36g, schizonepeta 13g ~ 23g, Centipeda minima 16g ~ 26g, oriental wormwood 21g ~ 31g, root of herbaceous peony 25g ~ 35g, Chinese violet 20g ~ 30g, radix bupleuri 19g ~ 29g, Chinese bulbul 26g ~ 36g, Gaultheria 17g ~ 27g, bog starwort herb 22g ~ 32g, eleusine indica 20g ~ 30g, dried immature fruit of citron orange 23g ~ 33g, folium sennae 18g ~ 28g, corydalis tuber 20g ~ 30g, cape jasmine 33g ~ 43g, Silence tenuis 15g ~ 25g and rhizoma Gastrodiae 20g ~ 30g.

Wherein, in described medicament additive, the weight of each bulk drug is respectively cordate houttuynia 25g ~ 29g, honeysuckle 46g ~ 50g, Gorgon fruit 20g ~ 24g, Chinese yam 29g ~ 33g, schizonepeta 16g ~ 20g, Centipeda minima 19g ~ 23g, oriental wormwood 24g ~ 28g, root of herbaceous peony 28g ~ 32g, Chinese violet 23g ~ 27g, radix bupleuri 22g ~ 26g, Chinese bulbul 29g ~ 33g, Gaultheria 20g ~ 24g, bog starwort herb 25g ~ 29g, eleusine indica 23g ~ 27g, dried immature fruit of citron orange 26g ~ 30g, folium sennae 21g ~ 25g, corydalis tuber 23g ~ 27g, cape jasmine 36g ~ 40g, Silence tenuis 18g ~ 22g and rhizoma Gastrodiae 23g ~ 27g.

Wherein, in described medicament additive, the weight of each bulk drug is respectively cordate houttuynia 27g, honeysuckle 48g, Gorgon fruit 22g, Chinese yam 31g, schizonepeta 18g, Centipeda minima 21g, oriental wormwood 26g, root of herbaceous peony 30g, Chinese violet 25g, radix bupleuri 24g, Chinese bulbul 31g, Gaultheria 22g, bog starwort herb 27g, eleusine indica 25g, dried immature fruit of citron orange 28g, folium sennae 23g, corydalis tuber 25g, cape jasmine 38g, Silence tenuis 20g and rhizoma Gastrodiae 25g.

Present invention also offers the preparation method of described medicament additive, be specially:

The first step, mixes by weight by each raw material components of medicament, and every 100g mixture, adds the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverizes, drying, obtains powder, adds maize cob meal subsequently with the ratio of mass ratio 1: 1, stir, obtain mixed-powder, be medicament additive.

Beneficial effect of the present invention:

Medicament additive provided by the invention is Be very effective in prevention chicken blackhead.

Detailed description of the invention

The invention provides a kind of medicament for preventing and treating chicken blackhead, its bulk drug comprises cordate houttuynia, honeysuckle, Gorgon fruit, Chinese yam, schizonepeta, Centipeda minima, oriental wormwood, the root of herbaceous peony, Chinese violet, radix bupleuri, the Chinese bulbul, Gaultheria, bog starwort herb, eleusine indica, the dried immature fruit of citron orange, folium sennae, corydalis tuber, cape jasmine, Silence tenuis and rhizoma Gastrodiae.

Further preferably, described medicament is only made up of above-mentioned raw materials medicine.

In described medicament, the weight of each bulk drug is respectively cordate houttuynia 22g ~ 32g, honeysuckle 43g ~ 53g, Gorgon fruit 17g ~ 27g, Chinese yam 26g ~ 36g, schizonepeta 13g ~ 23g, Centipeda minima 16g ~ 26g, oriental wormwood 21g ~ 31g, root of herbaceous peony 25g ~ 35g, Chinese violet 20g ~ 30g, radix bupleuri 19g ~ 29g, Chinese bulbul 26g ~ 36g, Gaultheria 17g ~ 27g, bog starwort herb 22g ~ 32g, eleusine indica 20g ~ 30g, dried immature fruit of citron orange 23g ~ 33g, folium sennae 18g ~ 28g, corydalis tuber 20g ~ 30g, cape jasmine 33g ~ 43g, Silence tenuis 15g ~ 25g and rhizoma Gastrodiae 20g ~ 30g.

Further preferably, in described medicament, the weight of each bulk drug is respectively cordate houttuynia 25g ~ 29g, honeysuckle 46g ~ 50g, Gorgon fruit 20g ~ 24g, Chinese yam 29g ~ 33g, schizonepeta 16g ~ 20g, Centipeda minima 19g ~ 23g, oriental wormwood 24g ~ 28g, root of herbaceous peony 28g ~ 32g, Chinese violet 23g ~ 27g, radix bupleuri 22g ~ 26g, Chinese bulbul 29g ~ 33g, Gaultheria 20g ~ 24g, bog starwort herb 25g ~ 29g, eleusine indica 23g ~ 27g, dried immature fruit of citron orange 26g ~ 30g, folium sennae 21g ~ 25g, corydalis tuber 23g ~ 27g, cape jasmine 36g ~ 40g, Silence tenuis 18g ~ 22g and rhizoma Gastrodiae 23g ~ 27g.

Most preferably, in described medicament, the weight of each bulk drug is respectively cordate houttuynia 27g, honeysuckle 48g, Gorgon fruit 22g, Chinese yam 31g, schizonepeta 18g, Centipeda minima 21g, oriental wormwood 26g, root of herbaceous peony 30g, Chinese violet 25g, radix bupleuri 24g, Chinese bulbul 31g, Gaultheria 22g, bog starwort herb 27g, eleusine indica 25g, dried immature fruit of citron orange 28g, folium sennae 23g, corydalis tuber 25g, cape jasmine 38g, Silence tenuis 20g and rhizoma Gastrodiae 25g.

Described medicament can be prepared into decoction liquor, is used for the treatment of chicken blackhead, also can as feed addictive, for preventing chicken blackhead.

Present invention also offers the preparation method of above-mentioned medicament, when the formulation of described medicament is decoction liquor, each raw material components in described medicament is mixed by weight, every 100g mixture, adds the water of 500g ~ 600g, boils 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boils 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, being concentrated into crude drug concentration is 1g/ml.

Present invention also offers the preparation method of above-mentioned medicament, when the formulation of described medicament is feed addictive, be specially:

The first step, mixes by weight by each raw material components of medicament, and every 100g mixture, adds the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverizes, drying, obtains powder, adds maize cob meal subsequently with the ratio of mass ratio 1: 1, stir, obtain mixed-powder, be medicament feed addictive.

Present invention also offers the feed for preventing and treating chicken blackhead, its raw material comprises medicament feed addictive, corn, wheat bran, dregs of beans, fish meal, multiple elements design vitamin, multiple elements design trace element, multiple elements design amino acid and salt for preventing chicken blackhead.

Further preferably, described feed is only made up of above-mentioned raw materials.

Wherein, in described feed, the weight of each raw material is respectively described for preventing medicament feed addictive 0.8kg ~ 1.2kg, the corn 60kg ~ 70kg of chicken blackhead, wheat bran 8kg ~ 10kg, dregs of beans 20kg ~ 30kg, fish meal 5kg ~ 10kg, multiple elements design vitamin 0.5kg ~ 1kg, the micro-0.6kg ~ 0.8kg of multiple elements design, multiple elements design amino acid 0.5kg ~ 1kg, salt 3kg ~ 5kg.

Further preferably, in described feed, the weight of each raw material is respectively medicament feed addictive 1kg, corn 70kg for preventing chicken blackhead, wheat bran 10kg, dregs of beans 25kg, fish meal 8kg, multiple elements design vitamin 0.8kg, multiple elements design trace element 0.7kg, multiple elements design amino acid 0.8kg, salt 4kg.

Present invention also offers the preparation method of above-mentioned feed, be specially:

The first step, prepares above-mentioned for preventing the medicament feed addictive of chicken blackhead;

Second step, is used for prevent the medicament feed addictive of chicken blackhead together with putting into fodder mixing machine described in being obtained by other raw material components in feedstuff with the first step, and continuous uniform stirred after 1 ~ 2 hour, put into pulverizer and pulverized, and obtains feed.

Described multiple elements design vitamin selection model is the vitamine supplement of prosperous diligent multidimensional-103, Weifang Heng Mao animal health-care product Co., Ltd provides, also can adopt complex vitamin premix conventional on other markets, vitamin mixing conventional to riboflavin, vitamin C, vitamin E, vitamin A, Cobastab etc. also can be selected to be added on together.

Described multiple elements design amino acid selects model to be the amino acid additive of Henkel, Shandong Henkel bioengineering Co., Ltd provides, also can adopt composite amino acid additive conventional on other markets, amino acid mixing conventional to serine, glutamic acid, glycine, isoleucine, leucine, alanine, proline etc. also can be selected to be added on together.

Described multiple elements design trace element selects model to be the micro-composite additive of trace elements of the excellent profit of 8818B, Beijing You Libao biotechnology Co., Ltd provides, also can adopt composite additive of trace elements conventional on other markets, trace element mixing conventional to ferrous sulfate, Copper lysinate, zinc methionine, manganese sulfate, sodium selenite, Potassiumiodate, cobalt chloride etc. also can be selected to be added on together.

Described each bulk drug pharmacology is as follows:

Cordate houttuynia: latin name houttuyniae herba, taste is pungent, cold nature, enters lung, urinary bladder channel, has clearing heat and detoxicating, removing pus and relieving carbuncle, effect of inducing diuresis for treating strangurtia, and for lung carbuncle pyemesis, phlegm heat is coughed and breathed heavily, toxic-heat and blood stasis, damp-heat gonorrhoea, hot carbuncle carbuncle, and snakeworm wants first-class card.

Honeysuckle: latin name lonicerae japonicae flos, taste is sweet, cold in nature, enters the heart, lung, stomach, large intestine channel, has clearing heat and detoxicating effect, doublely can remove heat from the blood and relieve diarrhea.Be mainly used in affection of exogenous wind-heat, the hot poison of warm heat disease is comparatively contained, or when warm disease is still not descending at the part of the body cavity above the diaphragm housing the heart and lungs from the beginning of heat evil; The malicious diarrhea of heat, the lower blood of stool; Heat temperature is demonstrate,proved; The sore carbuncle that hot poison gathers from the beginning of, lift the heat person of swelling and ache etc., honeysuckle has good antipyretic effect, energy anti-inflammatory, what control inflammation oozes out and inflammatory hyperplasia, leukocytic phagocytic function can be promoted, there is stronger bacteriostasis to multiple pyococcus and pathogenic entero becteria, comprise staphylococcus, hemolytic streptococcus, diplococcus meningitidis, shigella dysenteriae, typhoid bacillus, ewcastle disease, Pseudomonas aeruginosa, tubercle bacillus etc.

Gorgon fruit: taste is sweet, puckery, property is put down, and returns spleen, kidney channel.There is supplementing the kidney to control the nocturnal, tonifying spleen antidiarrheal, the function that clearing damp is only with, cure mainly emission, splenasthenic diarrhea, insufficiency of the spleen gonorrhoea, be with inferior card.

Chinese yam: latin name dioscoreae rhizoma, taste is sweet, property is put down, enter lung, spleen, kidney channel, have strengthening the spleen and tonifying the lung, beneficial stomach kidney tonifying, benefit of reinforcing the kidney essence, hearing-improing and eyesight improving, effect of helping the five internal organs, strengthening the bones and muscles, long will to calm the nerves, promote longevity, cure mainly weakness of the spleen and the stomach, lassitude, poor appetite, chronic diarrhea and dysentery, Lung Qi deficiency are dry, asthma due to excessive phlegm cough, kidney qi loss, soreness and weakness of waist and knees, lower limb impotence are weak, frequent micturition of quenching one's thirst, seminal emission premature ejaculation, band descend the red card such as swollen, fat of gonorrhoea, skin.

Schizonepeta: taste is pungent, slightly warm in nature, enters lung, Liver Channel, has and expel pathogenic wind from the body surface, and declares malicious promoting eruption, and regulating blood condition stops effect of convulsion, cures mainly flu fever and chills, headache, eye itching, pharyngalgia, cough, measles, rubella, carbuncle sore, brigadier, spit blood, clot blood, has blood in stool, uterine bleeding, the card such as affection by cold after delivery, bruise.

Centipeda minima: taste is pungent, warm in nature, enter lung, Liver Channel, there is cold-dispelling, clearing the nasal passage, cough-relieving apophlegmatic, the effect of eliminating stasis to subdue swelling, for exterior syndrome due to wind-cold, nasal obstruction runny nose and nasosinusitis, coughing with a lot of sputum, pertussis, the cards such as sore, carbuncle and painful swelling.

Oriental wormwood: latin name Artemisiae Scopariae Herba, bitter, pungent, cold nature, enter spleen, stomach, liver, gallbladder channel, there is clearing away damp-heat, the effect of removing jaundice subcutaneous ulcer, for jaundice, no matter damp and hot yang jaundice or cold-dampness yin jaundice all can be applied, and also can be applicable to the cards such as scanty drak urine caused by heat temperature or damp-warm syndrome.

The root of herbaceous peony: latin name Paeoniae Radix Alba, bitter, acid, cool in nature; enter liver, the spleen channel, there is nourishing blood and liver, pain relieving in slow; astringe yin receives effect of sweat, cures mainly chest abdomen pain over the hypochondriac region, rushes down dysentery stomachache; spontaneous sweating; fever due to yin deficiency, irregular menstruation, uterine bleeding; be with inferior card, the root of herbaceous peony has good liver-protective effect.

Chinese violet: bitter, pungent, cold in nature, enter the heart, Liver Channel, there is the effect of clearing heat and detoxicating, cool blood detumescence, be mainly used in the treatment for the treatment of malignant boil carbuncle sore furuncle, also have good therapeutic effect to acute conjunctivitis, sty etc., Chinese violet has inhibitory action in various degree to Gold production staphylococcus, pneumobacillus, Escherichia coli, alpha streptococcus, beta streptococcus, Bacillus influenzae, corynebacterium diphtheriae, Pseudomonas aeruginosa, white grapes sugar coccus, Candida albicans.

Radix bupleuri: latin name bupleuri radix, for the dry root of umbelliferae bupleurum or radix bupleuri scorzoneraefolii, bitter, cold nature, return Liver Channel, gallbladder channel, there is the effect of evacuating the easypro liver of sun of bringing down a fever, rise, for cold, fever, fevers and chills alternate, malaria, stagnation of QI due to depression of the liver, sternal rib pain, prolapse of the anus, the cards such as uterus comes off, irregular menstruation.

The Chinese bulbul: bitter, cold in nature, enter stomach, large intestine channel, there is effect that is clearing heat and detoxicating, that remove heat from the blood and relieve diarrhea, the treatment of the symptoms such as being mainly used in stomach has damp and hot stagnant heating to suffer from abdominal pain, diarrhea purulence blood, tenesmus, better to blood effect under the hot dysentery of large intestine blood-head, the Chinese bulbul all has certain inhibitory action to shigella dysenteriae, typhoid bacillus, hay bacillus, Escherichia coli, Gold production staphylococcus.

Gaultheria: be root or the cauline leaf of the white strain tree of Ericaceae Gaultheria plant, taste is pungent, puckery, warm in nature, and gas fragrance, has the effect of dispelling rheumatism, cure mainly rheumatic arthritis, the cards such as traumatic injury.

Bog starwort herb: be the herb of Caryophyllaceae Stellaria plant tongue of sparrow grass, taste is pungent, property is put down, and returns lung, the spleen channel, has and dispel rheumatism, activating blood circulation and reducing swelling, and effect of removing toxic substances hemostasis, cures mainly flu, rushes down dysentery, arthralgia due to wind-dampness, traumatic injury, venomous snake bite, spits blood, the cards such as traumatism and bleeding.

Eleusine indica: be root or the herb of grass family yard grass platymiscium eleusine indica, taste is sweet, light, cool in nature, has clearing heat and detoxicating, dampness removing, effect of cool the blood dissipate blood stasis, cure mainly and hinder heat heating, child convulsion, encephalitis, epidemic meningitis, jaundice, stranguria; difficult urination, dysentery, has blood in stool, nettle rash, the cards such as traumatic injury.

The dried immature fruit of citron orange: bitter, pungent, cold in nature, enters spleen, stomach, liver, the heart channel of Hang-Shaoyin, has relieving stagnant Qi to disappear long-pending, dissolving phlegm except the effect of ruffian, be mainly used in stagnant in stop, the YangmingFushi syndrome of abdominal fullness and distention, constipation; The taste stagnation of the circulation of vital energy, phlegm wet place food stagnate, the treatment of the symptoms such as the phlegm and retained fluid obstruction of qi in the chest of mechanism of qi impatency.

Folium sennae: taste is sweet, bitter, cold in nature, enters large intestine channel, has purgating heat and bowels, the merit of the stagnant sharp water of row, be mainly used in thermojunction and stagnate the constipation of stomach, the abdominal distension abdomen of accumulation of food in the stomach and intes tine due to indigestion is full, the symptoms such as do not feel like eating.

Corydalis tuber: have another name called corydalis tuber, rhizoma corydalis, corydalis tuber, taste is pungent, bitter, warm in nature, enters liver, the spleen channel, there is promoting blood circulation to remove blood stasis, the effect of promoting qi circulation and relieving pain, for the various pains card that qi depression to blood stasis causes, particularly trusted subordinate's pain commonly use, as depressed liver and heat transmission, the gastral cavilty abdominal distension of liver-stomach disharmony, has analgesic effect.

Cape jasmine: latin name gardeniae fructus, bitter, cold in nature, enter the heart, lung, three burnt, have the effect of relieving restlessness purging intense heat, soothing liver-qi stagnation, clearing heat and promoting diuresis, cooling blood and hemostasis, be mainly used in the vexed uneasiness of the strongly fragrant chest gastral cavity of warm heat disease heat, bother not peaceful; Liver depression is not relaxed or Liver Channel has having a dizzy spell of heat, pain in chest and hypochondrium, hot eyes dry throat; Jaundice with damp-heat pathogen; The cards such as the abdominal distention of qi depression to blood stasis.

Silence tenuis: bitter, pungent, property is put down, and has clearing heat and promoting diuresis, promoting blood circulation for regulating menstruation, effect of hemostasis, cures mainly heat and drenches, difficult urination, dysentery, irregular menstruation, through closing, uterine bleeding, the cards such as traumatism and bleeding.

Rhizoma Gastrodiae: taste is sweet, property is flat, enters Liver Channel, has calming the liver to stop the wind, spasmolysis, the effect of removing obstruction in channels to relieve pain, is mainly used in the dizzy headache of liver-fire flaming, the card such as strong wind tic, epilepsy that liver-yang hyperactivity, liver wind agitation are caused.

Below adopt embodiment to describe embodiments of the present invention in detail, to the present invention, how application technology means solve technical problem whereby, and the implementation procedure reaching technique effect can fully understand and implement according to this.

Embodiment 1 decoction liquor 1

By cordate houttuynia 27g, honeysuckle 48g, Gorgon fruit 22g, Chinese yam 31g, schizonepeta 18g, Centipeda minima 21g, oriental wormwood 26g, root of herbaceous peony 30g, Chinese violet 25g, radix bupleuri 24g, Chinese bulbul 31g, Gaultheria 22g, bog starwort herb 27g, eleusine indica 25g, dried immature fruit of citron orange 28g, folium sennae 23g, corydalis tuber 25g, cape jasmine 38g, Silence tenuis 20g and rhizoma Gastrodiae 25g mixes, every 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, be concentrated into the decoction liquor 1 that crude drug concentration is 1g/ml.

Embodiment 2 decoction liquor 2

By cordate houttuynia 25g, honeysuckle 46g, Gorgon fruit 20g, Chinese yam 29g, schizonepeta 16g, Centipeda minima 19g, oriental wormwood 24g, root of herbaceous peony 28g, Chinese violet 23g, radix bupleuri 22g, Chinese bulbul 29g, Gaultheria 20g, bog starwort herb 25g, eleusine indica 23g, dried immature fruit of citron orange 26g, folium sennae 21g, corydalis tuber 23g, cape jasmine 36g, Silence tenuis 18g and rhizoma Gastrodiae 23g mixes, every 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, be concentrated into the decoction liquor 2 that crude drug concentration is 1g/ml.

Embodiment 3 decoction liquor 3

By cordate houttuynia 29g, honeysuckle 50g, Gorgon fruit 24g, Chinese yam 33g, schizonepeta 20g, Centipeda minima 23g, oriental wormwood 28g, root of herbaceous peony 32g, Chinese violet 27g, radix bupleuri 26g, Chinese bulbul 33g, Gaultheria 24g, bog starwort herb 29g, eleusine indica 27g, dried immature fruit of citron orange 30g, folium sennae 25g, corydalis tuber 27g, cape jasmine 40g, Silence tenuis 22g and rhizoma Gastrodiae 27g mixes, every 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, be concentrated into the decoction liquor 3 that crude drug concentration is 1g/ml.

Embodiment 4 medicament feed addictive 1

By cordate houttuynia 27g, honeysuckle 48g, Gorgon fruit 22g, Chinese yam 31g, schizonepeta 18g, Centipeda minima 21g, oriental wormwood 26g, root of herbaceous peony 30g, Chinese violet 25g, radix bupleuri 24g, Chinese bulbul 31g, Gaultheria 22g, bog starwort herb 27g, eleusine indica 25g, dried immature fruit of citron orange 28g, folium sennae 23g, corydalis tuber 25g, cape jasmine 38g, Silence tenuis 20g and rhizoma Gastrodiae 25g mixes, every 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain powder, maize cob meal is added subsequently with the ratio of mass ratio 1: 1, stir, obtain mixed-powder, be medicament feed addictive 1.

Embodiment 5 medicament feed addictive 2

By cordate houttuynia 25g, honeysuckle 46g, Gorgon fruit 20g, Chinese yam 29g, schizonepeta 16g, Centipeda minima 19g, oriental wormwood 24g, root of herbaceous peony 28g, Chinese violet 23g, radix bupleuri 22g, Chinese bulbul 29g, Gaultheria 20g, bog starwort herb 25g, eleusine indica 23g, dried immature fruit of citron orange 26g, folium sennae 21g, corydalis tuber 23g, cape jasmine 36g, Silence tenuis 18g and rhizoma Gastrodiae 23g mixes, every 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain powder, maize cob meal is added subsequently with the ratio of mass ratio 1: 1, stir, obtain mixed-powder, be medicament feed addictive 2.

Embodiment 6 medicament feed addictive 3

By cordate houttuynia 29g, honeysuckle 50g, Gorgon fruit 24g, Chinese yam 33g, schizonepeta 20g, Centipeda minima 23g, oriental wormwood 28g, root of herbaceous peony 32g, Chinese violet 27g, radix bupleuri 26g, Chinese bulbul 33g, Gaultheria 24g, bog starwort herb 29g, eleusine indica 27g, dried immature fruit of citron orange 30g, folium sennae 25g, corydalis tuber 27g, cape jasmine 40g, Silence tenuis 22g and rhizoma Gastrodiae 27g mixes, every 100g mixture, add the water of 600g, boil 4 hours, filter, obtain the first filtrate, filter residue adds the water of 300g again, again boil 2 hours, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverize, dry, obtain powder, maize cob meal is added subsequently with the ratio of mass ratio 1: 1, stir, obtain mixed-powder, be medicament feed addictive 3.

Embodiment 7 toxicology test

Acute toxicity testing

Select Kunming mouse 20, female half and half, body weight (20+2) g, is provided by Qingdao City's Experimental Animal Center, and the decoction liquor 1 embodiment 1 prepared gavages mouse, through preliminary experiment, fails to detect the LD of oral administration ₅₀, therefore survey its maximal tolerance dose, with the administration volume of 2.0ml/10g body weight, gavage 2 times/d, interval 6h, to observation post administration 7d, record animal dead and toxicity symptom, at the end of experiment, by all animals process, dissect, visually observe each main organs lesion tissue situation, after gavaging 140g crude drug/kg for 2 times, animal activity, feed water inlet does not show any abnormalities, and observe 7d animal dead, gross necropsy has no the obvious exception of main organs tissue.

Long term toxicity test

Select 40 male SD rats, weight (180 ± 20) g, thered is provided by Qingdao City's Experimental Animal Center, be divided into 2 groups at random, blank group and medicament group, often organize 20, two groups of feeding conventional feeds, blank group gives distilled water, and decoction liquor prepared by embodiment 1 is gavaged rat by medicament group, and the amount of gavaging is 50g crude drug/kg, on every Mondays to gastric infusion 1 time in morning Saturday, continuous 180d, administration volume is 2mL/100g body weight, and every day observes rat general state, sign, food ration, drinking-water and ight soil situation before administration and during administration.During administration 90d after last administration 24h, the extraction 6 of each group, male and female half and half; During 180d after last administration 24h, the extraction 6 of each group, male and female half and half; After drug withdrawal, 30d respectively organizes extraction 6, male and female half and half, anaesthetize with urethane, blood is got from abdominal aortic, each group is all killed and gets animal, checks hematology and blood parameters, after getting blood, amass wealth by heavy taxation at once core, liver,kidney,spleen, lung, adrenal gland, thyroid gland, thymus gland, testis, ovary, uterus, brain, the internal organs such as prostate weigh, and make pathological examination according to weight calculator official weight coefficient.

On inspection, during successive administration, the activity of animal, body weight, food ration, stool and hair luster degree no abnormality seen, animal has no dead, naked eyes have no obvious pathology, each group of rat peripheral hemogram checking is all in normal range (NR), and the raw tea index of blood is all in normal range (NR), and medicament group Rats Organs and Tissues coefficient does not have significant difference compared with blank group.

Show through toxicology test, medicament provided by the invention is nontoxic.

The preparation of embodiment 8 feed 1

The 1kg medicament feed addictive 1 embodiment 4 prepared, corn 70kg, wheat bran 10kg, dregs of beans 25kg, the diligent multidimensional-103 of fish meal 8kg, 0.8kg multiple elements design vitamin, 0.7kg multiple elements design trace element 8818B excellent profit are micro-, multiple elements design amino acid Henkel 0.8kg, salt 4kg put into fodder mixing machine, after continuous uniform stirs 2 hours, put into pulverizer to pulverize, obtain feed 1.

The preparation of comparative example 1 feed 2

Micro-to corn 70kg, wheat bran 10kg, dregs of beans 25kg, the diligent multidimensional-103 of fish meal 8kg, 0.8kg multiple elements design vitamin, 0.7kg multiple elements design trace element 8818B excellent profit, multiple elements design amino acid Henkel 0.8kg, salt 4kg are put into fodder mixing machine, after continuous uniform stirs 2 hours, put into pulverizer to pulverize, obtain feed 2.

Prevention effect is tested

For examination animal: the healthy broiler chicken totally 500 of 30 ages in days, is divided into 6 groups, often organizes 100, be respectively treatment group, prevention group, blank group, positive controls 1, positive controls 2 and blind liver spirit control group.

Cause of disease material: take from certain poultry specialist, be diagnosed as the sick chicken of blackhead, get the pathological tissues of its caecum and liver, grind to form the emulsion that homogenate makes 1: 5, adds and dual anti-ly makes every milliliter containing 1000 μ L, be contained in seal in the vaccine bottle of the 100mL of sterilizing for subsequent use.

The preparation of cause of disease liquid: cause of disease tissue fluid emulsion is inoculated in 37 DEG C of cultivation 72h in special culture media, draw sediment bottom test tube and move to second time Multiplying culture in new culture medium, so repeatedly for several times, microscopy numeration at any time, treat that culture medium inner tissue trichomonad number reaches 2.5 ten thousand/mL nutrient solution, put in sterilization container to be put in 4 DEG C of refrigerators and save backup.

Test drug: decoction liquor 1 prepared by embodiment 1, feed 1 prepared by embodiment 8, feed 2 prepared by comparative example 1, blind liver spirit, lot number 20031204, specification 250g/ bag, Zhengzhou Zhengzhou animal pharmaceutical factory of Ya Wei industry parent company produces.Recommendation dosage is in 1% ratio spice mixed feeding.

Attack poison morbidity test

Speak in a low voice chicken every the crop of waiting to attack poison interior injection above-mentioned cause of disease liquid 0.5mL, and the 0.2mL/ of anus inoculation simultaneously only, waits for morbidity.

Prevention group starts feed 1 prepared by feeding embodiment 8 when on-test, carry out challenge test until feeding after 5 days, continues the feed 1 keeping feeding embodiment 8 to prepare simultaneously, continues 5 days; Positive controls 1 starts feed 2 prepared by feeding comparative example 1 when on-test, carry out challenge test until feeding after 5 days, continues the feed 2 keeping feeding comparative example 1 to prepare simultaneously, continues 5 days; The blank group feed 2 that when on-test prepared by feeding comparative example 1, does not carry out any challenge test, continues 10 days; The treatment group feed 2 that when on-test prepared by feeding comparative example 1, start challenge test, after 3 days there is illness in all test chickens simultaneously, be diagnosed as chicken blackhead, while continuing the feed 2 of feeding comparative example 1 preparation, gavage decoction liquor 1 prepared by embodiment 1, every day, 25ml, continued 5 days; Positive controls 2 feed 2 that when on-test prepared by feeding comparative example 1, start challenge test, after 3 days there is illness in all test chickens simultaneously, is diagnosed as chicken blackhead, continues feed 2 prepared by feeding comparative example 1, continues 5 days; The blind liver spirit control group feed 2 that when on-test prepared by feeding comparative example 1, start challenge test, after 3 days there is illness in all test chickens simultaneously, continues feed 2 prepared by feeding comparative example 1, simultaneously by 1% ratio by clever for blind liver spice mixed feeding.

Attack malicious success indicator: suffer from chicken lassitude, chilly/subtract food, feather pine is random/matt.Have loose bowels, first passage of watery stools, arrange successively subsequently bloody stool/yellowish-white colour band bloody stool/yellowish-brown just/sulphur look just/black paste ight soil, its excrement stench.Suffer from cock skin skin cyanosis, it is blunt to show, and slow-witted on one side vertical, two wings are sagging, and what have is curved in wing, in stilt walk sample gait during walking.Phase suffers from chicken stomach inanition after being ill, and day by day become thin, health is rolled up, and what have can touch allantoid caecum at belly.Have spasm before short dead of the course of disease or performance of struggling, temperature decline before long dead of the course of disease, does not rise sleepingly.This sufferer chicken with desulphurization sulphur look ight soil and stilt walk sample gait for feature.

Curative effect determinate standard:

Recovery from illness: test chicken spirit, diet wish, ight soil recover normal, cuts open inspection caecum inner blood and cheesy thing is discharged, and caecum and hepatic necrosis are repaired substantially, the inert tissue trichomonad of pathological tissues microscopy.Effective: test chicken spirit, diet wish take a turn for the better, and blood stool reduces, cut open cheesy thing and blood major part discharge in inspection caecum, the ulcer of caecum and liver and necrosis region have started to repair, and pathological tissues microscopy is movable without polypide.Invalid: test chicken spirit diet is for having no improvement, blood stool is serious, volume blood and cheesy thing is had in caecum, the necrosis region of cecum mucosa and submucosal popularity ulcer and liver surface has no reparation, or occurring dead; pathological tissues microscopy has a large amount of tissue trichomonads, rotates rapidly or clock pendulum-like movement in original place.

Data processing

Measurement data adopts represent, compare between group and adopt χ ²inspection, all data all adopt the process of SPSS12.0 statistical software.

Result

Preventive effect compares, in table 1.

The overall preventive effect of table 1 compares

Group	Total quantity	Morbidity number	Incidence of disease %
				Prevention group	100	13	13％
Positive controls	100	100	100％

As can be seen from Table 1, pharmaceutical preparation of the present invention is respond well to prevention chicken blackhead aspect.

Overall treatment effect, in table 2.

Table 2 respectively group chicken overall treatment effect compares

As can be seen from Table 2, the treatment of medicament provided by the invention to chicken blackhead is adopted to have good effect.

All above-mentioned this intellectual properties of primary enforcement, not setting restriction this new product of other forms of enforcement and/or new method.Those skilled in the art will utilize this important information, and foregoing is revised, to realize similar implementation status.But all modifications or transformation belong to the right of reservation based on new product of the present invention.

The above is only preferred embodiment of the present invention, and be not restriction the present invention being made to other form, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed or be modified as the Equivalent embodiments of equivalent variations.But everyly do not depart from technical solution of the present invention content, any simple modification, equivalent variations and the remodeling done above embodiment according to technical spirit of the present invention, still belong to the protection domain of technical solution of the present invention.

Claims (5)

1., for preventing a medicament additive for chicken blackhead, it is characterized in that: bulk drug comprises cordate houttuynia, honeysuckle, Gorgon fruit, Chinese yam, schizonepeta, Centipeda minima, oriental wormwood, the root of herbaceous peony, Chinese violet, radix bupleuri, the Chinese bulbul, Gaultheria, bog starwort herb, eleusine indica, the dried immature fruit of citron orange, folium sennae, corydalis tuber, cape jasmine, Silence tenuis and rhizoma Gastrodiae.

2. medicament additive as claimed in claim 1, it is characterized in that: the weight of each bulk drug is respectively cordate houttuynia 22g ~ 32g, honeysuckle 43g ~ 53g, Gorgon fruit 17g ~ 27g, Chinese yam 26g ~ 36g, schizonepeta 13g ~ 23g, Centipeda minima 16g ~ 26g, oriental wormwood 21g ~ 31g, root of herbaceous peony 25g ~ 35g, Chinese violet 20g ~ 30g, radix bupleuri 19g ~ 29g, Chinese bulbul 26g ~ 36g, Gaultheria 17g ~ 27g, bog starwort herb 22g ~ 32g, eleusine indica 20g ~ 30g, dried immature fruit of citron orange 23g ~ 33g, folium sennae 18g ~ 28g, corydalis tuber 20g ~ 30g, cape jasmine 33g ~ 43g, Silence tenuis 15g ~ 25g and rhizoma Gastrodiae 20g ~ 30g.

3. medicament additive as claimed in claim 1 or 2, it is characterized in that: the weight of each bulk drug is respectively cordate houttuynia 25g ~ 29g, honeysuckle 46g ~ 50g, Gorgon fruit 20g ~ 24g, Chinese yam 29g ~ 33g, schizonepeta 16g ~ 20g, Centipeda minima 19g ~ 23g, oriental wormwood 24g ~ 28g, root of herbaceous peony 28g ~ 32g, Chinese violet 23g ~ 27g, radix bupleuri 22g ~ 26g, Chinese bulbul 29g ~ 33g, Gaultheria 20g ~ 24g, bog starwort herb 25g ~ 29g, eleusine indica 23g ~ 27g, dried immature fruit of citron orange 26g ~ 30g, folium sennae 21g ~ 25g, corydalis tuber 23g ~ 27g, cape jasmine 36g ~ 40g, Silence tenuis 18g ~ 22g and rhizoma Gastrodiae 23g ~ 27g.

4. the medicament additive as described in claims 1 to 3, is characterized in that: the weight of each bulk drug is respectively cordate houttuynia 27g, honeysuckle 48g, Gorgon fruit 22g, Chinese yam 31g, schizonepeta 18g, Centipeda minima 21g, oriental wormwood 26g, root of herbaceous peony 30g, Chinese violet 25g, radix bupleuri 24g, Chinese bulbul 31g, Gaultheria 22g, bog starwort herb 27g, eleusine indica 25g, dried immature fruit of citron orange 28g, folium sennae 23g, corydalis tuber 25g, cape jasmine 38g, Silence tenuis 20g and rhizoma Gastrodiae 25g.

5. the preparation method of medicament additive described in Claims 1-4, is characterized in that:

The first step, mixes by weight by each raw material components of medicament, and every 100g mixture, adds the water of 500g ~ 600g, boil 3 ~ 4 hours, filter, obtain the first filtrate, filter residue adds the water of 200g ~ 300g again, again boil 1 ~ 2 hour, filter, obtain the second filtrate, by the first filtrate and the mixing of the second filtrate, concentrated removing aqueous solvent, pulverizes, drying, obtains powder, adds maize cob meal subsequently with the ratio of mass ratio 1: 1, stir, obtain mixed-powder, be medicament additive.