CN104277132A - Method for extraction of peanut polysaccharide from low temperature peanut meal - Google Patents
Method for extraction of peanut polysaccharide from low temperature peanut meal Download PDFInfo
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- CN104277132A CN104277132A CN201310282923.6A CN201310282923A CN104277132A CN 104277132 A CN104277132 A CN 104277132A CN 201310282923 A CN201310282923 A CN 201310282923A CN 104277132 A CN104277132 A CN 104277132A
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Abstract
The invention relates to a method for extraction of peanut polysaccharide from low temperature peanut meal. The low temperature peanut meal and ethanol solution are mixed, fully stirred, then centrifuged, and decanted to obtain supernatant; the supernatant is concentrated in vacuum, ethanol is recovered and supernatant, while the supernatant is concentrated, and the concentrated supernatant is dried by spraying to obtain crude peanut polysaccharide. The ethanol used in the method can be recycled, the cost of production is significantly decreased, environmental pollution caused in the leaching extraction process with commonly used acid and alkali solutions can be solved, the problems that enzyme preparation cost is too high and a large amount of water is consumed in the bio enzyme preparation method can be effectively avoided, by the spray drying technology, anhydrous ethanol or acetone washing process in the traditional process can be omitted, the cost of raw material is saved, and the production efficiency is improved.
Description
Technical field
The present invention relates to a kind of method extracting peanut polysaccharide from low temperature peanut meal, belong to biochemical field.
Background technology
Low temperature pressing peanut meal is the by product of low temperature pressing peanut oil, because low temperature destroys less to its effective constituent such as the polysaccharide be rich in and protein, has good functional performance and high value of exploiting and utilizing.Many natural radioactivity polysaccharide, are often referred to non-starch polysaccharide, have biological function widely, have been successfully applied to medicine, clinical and protective foods.Peanut meal contains more polysaccharide, and research shows that peanut polysaccharide has effect that is hypoglycemic and protection liver injury, therefore extracts and studies peanut polysaccharide and have realistic meaning, and contribute to improving cold press peanut meal comprehensive utilization ratio, increase its economic value added
At present, from peanut meal, extracting the method that peanut polysaccharide adopts mostly is: with acid or alkali lixiviate peanut polysaccharide, adopt ultrasonic wave or microwave method assisted extraction peanut polysaccharide; Biological enzyme formulation technology enzyme solution extracts peanut polysaccharide.
As Wang Chengming discloses " a kind of polysaccharide of extracting from peanut meal and extracting method thereof " at ZL200710052936.9, the method adopts solvent-extraction process, raw material peanut meal is added conventional acid, alkaline solution as extracting solution, after centrifugation precipitation, obtain the solution after being separated; In this solution, add the appropriate solvent that can make polysaccharide precipitation, polysaccharide is fully precipitated; Finally precipitated liquid is carried out centrifugal or filtration treatment, be precipitated thing, with dehydrated alcohol or acetone repetitive scrubbing several, lyophilize can obtain rough polysaccharide.
Domestic in ultrasonic extraction peanut polysaccharide also have many people to study, and wherein the people such as Xue Fang will disclose " research that extraction with ultrasonic-NaOH gets peanut polysaccharide " in volume the 8th phase in " Food science " 2008 end of the year 29; The people such as Yang Wei also disclosed " technical study of ultrasonic wave water extraction peanut meal polysaccharides " in " Chinese oil " the 32nd volume second phase in 2010, the hyperacoustic Mechanical Crushing of main employing and cavatition can effective broken vegetable cells, accelerate extractive substance from raw material to the rate of diffusion of solvent, its effective constituent in rapid extraction plant, by carrying out ultrasonic lixiviate process to the mixing solutions of peanut meal, centrifugal, get after supernatant liquor carries out alcohol precipitation, precipitate in centrifuging and taking, after precipitation dissolves constant volume with distilled water, the extraction yield recording peanut polysaccharide is 1.77%.
Duan Youchen discloses " a kind of method extracting peanut polysaccharide from hot pressed peanut meal " at ZL201110097588.3, by adopting cellulase, papoid and aspartic protease, the enzyme centrifugation of then going out of abundant enzymolysis is carried out to the hot pressed peanut meal of pre-treatment and obtain supernatant liquor, carry out concentrated after add trichloroacetic acid precipitation and again carry out centrifugation, the ethanolic soln adding 95% after getting supernatant liquor carries out alcohol precipitation, is precipitated then to carry out lyophilize and obtain peanut polysaccharide after recentrifuge.
Above method, uses strong acid or highly basic to there is contaminate environment, consume a large amount of water, and solvent cannot reclaim, and simultaneously because acid base concentration is high, is easy to the structure destroying natural radioactivity polysaccharide, and then reduces biological activity.The food safety part of product is also under suspicion: splendid sound wave produces that to there is facility investment excessive, and energy consumption is large, and commercial application exists certain defect; The zymin cost that biological enzyme formulation method exists reaction is high, and explained hereafter is discontinuous, the lower problem of efficiency, makes the production cost of product very high.Therefore need to design a kind of security for above problem high, production cost is low, environmentally friendly, the Novel peanut extraction method of polysaccharides that process continuity is strong.
Summary of the invention
In order to solve the problem in such scheme, the present invention is easy to precipitation by utilizing protein in low temperature peanut meal in certain density ethanolic soln, polysaccharide is dissolved in again the characteristic of ethanol simultaneously, then by the protein in centrifugal removing peanut meal, then carries out vacuum concentration and spraying dry.This technique improves the biological activity of polysaccharide to greatest extent, avoids the pollution of organic solvent.
The object of the method is to provide a kind of method extracting peanut polysaccharide from low temperature peanut meal, has effectively simple, the advantage that cost is low and easy to implement.Its concrete steps taked are as follows:
(1) alcohol extracting: get low temperature peanut meal raw material, the concentration that solid-liquid ratio according to 1: 8 ~ 1: 15 adds as extracting solution is 55% ~ 70% ethanolic soln, and then 30 ~ 50 DEG C is under the condition of 150 ~ 260r/min with stirring velocity, fully stirs 4 ~ 5hr;
(2) centrifugal decant: the alcohol mixed solution after fully stirring is carried out centrifugation, gets supernatant liquor after decant, obtain the solution containing peanut polysaccharide;
(3) vacuum concentration: supernatant liquor is carried out vacuum concentration, removes the ethanol in supernatant liquor and cool solidifying recovery;
(4) spraying dry: rough peanut polysaccharide can be obtained after spray-dried for the solution after concentration and evaporation.
The present invention compared with prior art tool has the following advantages:
1, utilize the solubility characteristics that the components such as albumen, starch, polysaccharide in low temperature peanut meal are different in ethanolic soln, extract peanut polysaccharide, reaction conditions is gentle, can reduce the infringement of solvent to peanut polysaccharide physiologically active to greatest extent;
2, the ethanol used in the method can carry out recycling use, obvious reduction production cost, solve the environmental pollution caused in common acid, alkaline solution leaching process, effectively prevent zymin high cost in biological enzyme formulation method and consume a large amount of water problem simultaneously;
3, the method adopts spray drying technology, saves the operation of the several washing of dehydrated alcohol or acetone in traditional technology (as vacuum freezedrying etc.), has saved raw materials cost and heightened production efficiency;
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, so that those skilled in the art more understand the present invention, but do not limit the present invention with this.
The experimental technique used in following embodiment if no special instructions, is ordinary method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Protein determination: GB/T5009,5-2003
Moisture determination: GB/T5009,3-2003
Fat test: GB/T5009,4-2003
Total sugar determination: phend-sulphuric acid
Embodiment 1,
Get the low temperature peanut meal (ash oontent 4.85% of 100 kilograms, protein content 55.88%, lipid content 1.50%, total sugar content 25.40%), according to the feed liquid of 1:10 also than adding the ethanolic soln that 1000L concentration is 60%, at 50 DEG C, when stirring velocity is 200r/m, abundant stirring 1.5h, then this mixing solutions is carried out centrifugation, obtain supernatant liquor and precipitation (protein content 69% in precipitation after measured respectively, ), impurity is removed after getting supernatant liquid filtering, then the liquid after filtering is passed through vacuum rotary evaporator (vacuum tightness 3800pa, vaporization temperature 50 DEG C) carry out reduction vaporization, ethanol is volatilized and reclaims, treat that the degree Beaume of target elutriant reaches 45 ° of B é, enter spray-drying tower (blast inlet 180 DEG C, air outlet 80 DEG C, rotating speed 18000r/min), obtain rough peanut polysaccharide powder 4.2kg (glucose content 1.64%: fructose content 2.39%: sucrose content 30.55%: Crude polysaccharides content 8.55%).
Embodiment 2,
Get the low temperature peanut meal of 200 kilograms, feed liquid according to 1: 15 is also than adding the ethanolic soln that 3000L concentration is 70%, at 45 DEG C, when stirring velocity is 150r/m, abundant stirring 2.0h, then this mixing solutions is carried out centrifugation, obtain supernatant liquor and precipitation (after measured protein content 70.5% in precipitation) respectively, impurity is removed after getting supernatant liquid filtering, then the liquid after filtering is passed through vacuum rotary evaporator (vacuum tightness 4000pa, vaporization temperature 55 DEG C) carry out reduction vaporization, ethanol is volatilized and reclaims, treat that the degree Beaume of target elutriant reaches 50 ° of B é, enter spray-drying tower (blast inlet 170 DEG C, air outlet 80 DEG C, rotating speed 19000r/m), obtain rough peanut polysaccharide powder 8.4kg (glucose content 1.55%: fructose content 2.19%: sucrose content 31.49%: Crude polysaccharides content 8.69%).
Claims (3)
1. the method extracting peanut polysaccharide from low temperature peanut meal is characterized in that: comprise the following steps:
(1) alcohol extracting: get low temperature peanut meal raw material, add ethanolic soln and fully stir;
(2) centrifugation: the alcohol mixed solution after fully stirring is carried out centrifugation, gets supernatant liquor after decant, obtain the solution containing peanut polysaccharide;
(3) vacuum concentration: supernatant liquor is carried out vacuum concentration, the ethanol in removing supernatant liquor
(4) spraying dry: rough peanut polysaccharide can be obtained after supernatant liquor after concentration and evaporation is carried out spraying dry.
2. method according to claim 1, it is characterized in that: step (1) is 55% ~ 70% ethanolic soln according to the concentration that adds as extracting solution of solid-liquid ratio of 1: 8 ~ 1: 15, then, under 30 ~ 50 DEG C and stirring velocity are the condition of 150 ~ 260r/min, fully 1 ~ 2h is stirred.
3. method according to claim 1, it is characterized in that: by peanut polysaccharide concentrated solution that step (3) obtains in step (4), according to degree Beaume 40 ~ 50 ° of B é, intake air temperature 160 ~ 200 DEG C, spraying dry under rotating speed 17000 ~ 20000r/min condition.
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| CN201310282923.6A CN104277132A (en) | 2013-07-08 | 2013-07-08 | Method for extraction of peanut polysaccharide from low temperature peanut meal |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106243236A (en) * | 2016-07-28 | 2016-12-21 | 石雷 | A kind of preparation method of blood clam extract product |
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| CN102180984A (en) * | 2011-03-04 | 2011-09-14 | 山东省农业科学院农产品研究所 | Method for extracting peanut polysaccharide from hot pressed peanut meal |
| CN102199224A (en) * | 2011-03-22 | 2011-09-28 | 华中农业大学 | Method for producing peanut polysaccharides and peanut concentrated protein by using peanut meal |
| CN103130908A (en) * | 2013-03-07 | 2013-06-05 | 中国农业科学院农产品加工研究所 | Method for extracting peanut polysaccharide from cold pressed peanut cake |
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| CN101104647A (en) * | 2007-08-13 | 2008-01-16 | 华中农业大学 | Oolysaccharide extracted from groundnut meal and extracting method thereof |
| CN101575369A (en) * | 2009-06-25 | 2009-11-11 | 合肥工业大学 | Technique for separating and preparing rapeseed protein cogenerating rapeseed polyoses from the low-temperature cold pressing rapeseed dregs film |
| CN102180984A (en) * | 2011-03-04 | 2011-09-14 | 山东省农业科学院农产品研究所 | Method for extracting peanut polysaccharide from hot pressed peanut meal |
| CN102199224A (en) * | 2011-03-22 | 2011-09-28 | 华中农业大学 | Method for producing peanut polysaccharides and peanut concentrated protein by using peanut meal |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106243236A (en) * | 2016-07-28 | 2016-12-21 | 石雷 | A kind of preparation method of blood clam extract product |
| CN106243236B (en) * | 2016-07-28 | 2018-12-07 | 石雷 | A kind of preparation method of blood clam extract product |
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