CN102002112A - Process for extracting ginkgo leaf polysaccharide with aid of ultrasonic - Google Patents
Process for extracting ginkgo leaf polysaccharide with aid of ultrasonic Download PDFInfo
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- CN102002112A CN102002112A CN2010105690113A CN201010569011A CN102002112A CN 102002112 A CN102002112 A CN 102002112A CN 2010105690113 A CN2010105690113 A CN 2010105690113A CN 201010569011 A CN201010569011 A CN 201010569011A CN 102002112 A CN102002112 A CN 102002112A
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- ultrasonic
- ginkgo leaf
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- polysaccharide
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Abstract
The invention provides a process for extracting ginkgo leaf polysaccharide with the aid of ultrasonic. The extracting process is technically characterized by comprising the following steps of: drying ginkgo leaves (ginkgo leaves after autumn), crushing and sieving (40 meshes), performing ultrasonic treatment (the distilled water is used as a solvent, and the temperature is controlled at 80 DEG C), centrifuging (removing residue), performing vacuum concentration (till 1/10 before concentration), performing alcohol precipitation, centrifuging (taking sediment), and performing vacuum freezing and drying to obtain a crude polysaccharide product. The process has the advantages that: the ultrasonic is acted on the liquid to generate cavatition during production to cause bubble formation, increment and blasting compression so as to disperse a solid sample, increase the contact area between the sample and the extracting solvent, improve the transfer rate of a target substance from solid phase to liquid phase, shorten the extracting time, reduce energy consumption and improve the extracting rate.
Description
Technical field
The invention belongs to a kind of novel extraction Ginkgo Leaf polysaccharide process, particularly a kind of employing ultrasonic technology extracts the Ginkgo Leaf polysaccharide process.
Background technology
The Ginkgo Leaf polysaccharide has and delays senility, and immunomodulatory and antitumor action have potential pharmaceutical use and medicine research and development etc.And active substance concentrates on and smokes letones in the Ginkgo Leaf, and the method for extraction mainly contains water extraction and alcohol precipitation method and this enzyme process in conjunction with the water extracting alcohol method, and these methods not only extraction yield are not high, and consuming time.Waste energy.
Summary of the invention
The objective of the invention is the problem that exists in the above-mentioned technology, a kind of ultrasonic extraction Ginkgo Leaf polysaccharide process is provided
The object of the present invention is achieved like this: it is characterized in that: extraction process is as follows, Ginkgo Leaf (Ginkgo Leaf after autumn) → drying → crushing screening (40 order) → supersound process (distilled water is solvent, and temperature is controlled at 80 ℃) → centrifugal (removing slag) → vacuum concentration (to concentrating preceding 1/10) → alcohol precipitation → centrifugal (getting precipitation) → vacuum lyophilization → polysaccharide crude.
The invention has the beneficial effects as follows, ultrasonic wave acts on liquid and produces cavatition aborning, cause bubble formation, increase, the explosion compression, thereby solid sample is disperseed, increase the contact area between sample and the extraction solvent, improved target compound and transferred to the transfer rate of liquid phase, shorten the extraction time from solid phase, reduced energy consumption, the extraction yield height.
Embodiment:
The present invention will be further described below in conjunction with embodiment:
Raw material: adopt ginkgo leaf-fall after autumn
Instrument: the RE-85Z Rotary Evaporators, the JH223 visible spectrophotometer, SKB-II circulation ability of swimming is used vacuum pump more, DHG-9143 II type electric heating constant temperature air dry oven, JFSD-100-II pulverizer, JY92-II D ultrasonic cell disruptor.
Technical process: Ginkgo Leaf → drying after autumn → crushing screening (40 order) → supersound process (distilled water is solvent, and temperature is controlled at 80 ℃) → centrifugal (removing slag) → vacuum concentration (to concentrating preceding 1/10) → alcohol precipitation → centrifugal (getting precipitation) → vacuum lyophilization → polysaccharide crude.
The GBLP content assaying method: get the 100mgGBLP crude product, be settled to 100ml with distilled water, and the therefrom accurate 1.0ml equivalent capacity of drawing, both be Crude polysaccharides sample liquid, adopt anthrone-sulfuric acid process to measure polysaccharide content then, both got 0.6ml sample liquid and placed.
In practice, again through optimization process, when distilled water is extraction agent, temperature is controlled at 80 ℃ to extraction process, and the liquid material is than 25ml/g, ultrasonic time 45min, and ultrasonic power 250W, under these processing condition, the Ginkgo Leaf polysaccharide extract rate is 5.8%.
In practice, along with increasing of temperature, polysaccharide extract rate also can increase, but impurity also can increase, and is controlled at below 80 ℃ and is advisable.
Claims (3)
1. ultrasonic-assisted extraction Ginkgo Leaf polysaccharide process, it is characterized in that: extraction process is as follows, Ginkgo Leaf, → drying → crushing screening 40 orders → supersound process, distilled water is solvent, temperature is controlled at 80 ℃, and → centrifugal removing slag → vacuum concentration is to concentrating preceding 1/10 → alcohol precipitation → centrifuging and taking precipitation → vacuum lyophilization → polysaccharide crude.
2. GBLP content assaying method according to claim 1: get the 100mgGBLP crude product, be settled to 100ml with distilled water, and the therefrom accurate 1.0ml equivalent capacity of drawing, both be Crude polysaccharides sample liquid, adopt anthrone-sulfuric acid process to measure polysaccharide content then, both got 0.6ml sample liquid and placed.
3. a kind of ultrasonic-assisted extraction Ginkgo Leaf polysaccharide process according to claim 1 is characterized in that: when distilled water is extraction agent, temperature is controlled at 80 ℃, and the liquid material compares 25ml/g, ultrasonic time 45min, ultrasonic power 250W, under these processing condition, the Ginkgo Leaf polysaccharide extract rate is 5.8%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2010105690113A CN102002112A (en) | 2010-12-02 | 2010-12-02 | Process for extracting ginkgo leaf polysaccharide with aid of ultrasonic |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105690113A CN102002112A (en) | 2010-12-02 | 2010-12-02 | Process for extracting ginkgo leaf polysaccharide with aid of ultrasonic |
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| CN102002112A true CN102002112A (en) | 2011-04-06 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351961A (en) * | 2011-11-15 | 2012-02-15 | 重庆市秀山红星中药材开发有限公司 | Method for extracting rhizoma polygonati polysaccharide from stems and leaves of rhizoma polygonati |
| CN102813683A (en) * | 2011-06-09 | 2012-12-12 | 北京华夏众芳生物科技有限公司 | Gingko leaf extract capable of delaying senescence and preparation method and application thereof |
| CN103848920A (en) * | 2012-12-03 | 2014-06-11 | 上海工程技术大学 | Method for extracting isatis root polysaccharide under ultrasonic assistance |
| CN105079050A (en) * | 2015-10-09 | 2015-11-25 | 江苏贝斯康药业有限公司 | Method for preparing superfine powder of extract rich in ginkgo biloba seed polysaccharide |
| CN105175568A (en) * | 2015-10-23 | 2015-12-23 | 珠海夫大食品科技有限公司 | Ginkgo seed polysaccharide extraction method and ginkgo seed polysaccharide product |
| CN108383921A (en) * | 2018-04-08 | 2018-08-10 | 江苏师范大学 | A kind of Ginkgo biloba polysaccharide extracting method, and the inoxidizability assay method with it |
| CN110183543A (en) * | 2019-06-05 | 2019-08-30 | 江苏师范大学 | A kind of extracting method of gingko Thick many candies |
-
2010
- 2010-12-02 CN CN2010105690113A patent/CN102002112A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102813683A (en) * | 2011-06-09 | 2012-12-12 | 北京华夏众芳生物科技有限公司 | Gingko leaf extract capable of delaying senescence and preparation method and application thereof |
| CN102813683B (en) * | 2011-06-09 | 2013-12-04 | 北京华夏众芳生物科技有限公司 | Gingko leaf extract capable of delaying senescence and preparation method and application thereof |
| CN102351961A (en) * | 2011-11-15 | 2012-02-15 | 重庆市秀山红星中药材开发有限公司 | Method for extracting rhizoma polygonati polysaccharide from stems and leaves of rhizoma polygonati |
| CN103848920A (en) * | 2012-12-03 | 2014-06-11 | 上海工程技术大学 | Method for extracting isatis root polysaccharide under ultrasonic assistance |
| CN103848920B (en) * | 2012-12-03 | 2016-07-06 | 上海工程技术大学 | A kind of ultrasonic assistant extracts the method for Banlangen Polysaccharide |
| CN105079050A (en) * | 2015-10-09 | 2015-11-25 | 江苏贝斯康药业有限公司 | Method for preparing superfine powder of extract rich in ginkgo biloba seed polysaccharide |
| CN105175568A (en) * | 2015-10-23 | 2015-12-23 | 珠海夫大食品科技有限公司 | Ginkgo seed polysaccharide extraction method and ginkgo seed polysaccharide product |
| CN105175568B (en) * | 2015-10-23 | 2017-07-14 | 珠海夫大食品科技有限公司 | A kind of method for extracting gingko polysaccharide and products thereof |
| CN108383921A (en) * | 2018-04-08 | 2018-08-10 | 江苏师范大学 | A kind of Ginkgo biloba polysaccharide extracting method, and the inoxidizability assay method with it |
| CN110183543A (en) * | 2019-06-05 | 2019-08-30 | 江苏师范大学 | A kind of extracting method of gingko Thick many candies |
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Application publication date: 20110406 |