CN104254776B - 提取和储存核酸的方法和组合物 - Google Patents

提取和储存核酸的方法和组合物 Download PDF

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Publication number
CN104254776B
CN104254776B CN201380022584.6A CN201380022584A CN104254776B CN 104254776 B CN104254776 B CN 104254776B CN 201380022584 A CN201380022584 A CN 201380022584A CN 104254776 B CN104254776 B CN 104254776B
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Prior art keywords
solid matrix
rna
nucleic acid
cellulose
compositions
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Chinese (zh)
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CN104254776A (zh
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李兵
D.R.穆尔
E.L.克瓦姆
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GE Healthcare UK Ltd
Global Life Sciences Solutions Operations UK Ltd
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General Electric Co
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201380022584.6A 2012-04-30 2013-04-29 提取和储存核酸的方法和组合物 Active CN104254776B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids
US13/460076 2012-04-30
PCT/US2013/038576 WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Publications (2)

Publication Number Publication Date
CN104254776A CN104254776A (zh) 2014-12-31
CN104254776B true CN104254776B (zh) 2016-09-28

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CN201380022584.6A Active CN104254776B (zh) 2012-04-30 2013-04-29 提取和储存核酸的方法和组合物

Country Status (8)

Country Link
US (1) US9044738B2 (https=)
EP (1) EP2844755B1 (https=)
JP (3) JP6248095B2 (https=)
CN (1) CN104254776B (https=)
CA (1) CA2870038C (https=)
ES (1) ES2609763T3 (https=)
IN (1) IN2014DN08223A (https=)
WO (1) WO2013165870A1 (https=)

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CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
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Also Published As

Publication number Publication date
IN2014DN08223A (https=) 2015-05-15
ES2609763T3 (es) 2017-04-24
CA2870038A1 (en) 2013-11-07
US20130289265A1 (en) 2013-10-31
EP2844755A1 (en) 2015-03-11
JP2018068303A (ja) 2018-05-10
WO2013165870A1 (en) 2013-11-07
CA2870038C (en) 2021-08-03
EP2844755B1 (en) 2016-11-16
JP6248095B2 (ja) 2017-12-13
US9044738B2 (en) 2015-06-02
JP2015518569A (ja) 2015-07-02
CN104254776A (zh) 2014-12-31
JP2020092709A (ja) 2020-06-18
EP2844755A4 (en) 2015-03-11
JP6847365B2 (ja) 2021-03-24

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Effective date of registration: 20210906

Address after: England

Patentee after: Global life science solutions UK Ltd.

Address before: Buckinghamshire

Patentee before: GE HEALTHCARE UK Ltd.

Effective date of registration: 20210906

Address after: Buckinghamshire

Patentee after: GE HEALTHCARE UK Ltd.

Address before: New York State, USA

Patentee before: General Electric Co.