WO2013165870A1 - Methods and compositions for extraction and storage of nucleic acids - Google Patents

Methods and compositions for extraction and storage of nucleic acids Download PDF

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Publication number
WO2013165870A1
WO2013165870A1 PCT/US2013/038576 US2013038576W WO2013165870A1 WO 2013165870 A1 WO2013165870 A1 WO 2013165870A1 US 2013038576 W US2013038576 W US 2013038576W WO 2013165870 A1 WO2013165870 A1 WO 2013165870A1
Authority
WO
WIPO (PCT)
Prior art keywords
solid matrix
nucleic acids
sample
rna
buffer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2013/038576
Other languages
English (en)
French (fr)
Inventor
Bing Li
David Roger Moore
Erik Leeming KVAM
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
General Electric Co
Original Assignee
General Electric Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by General Electric Co filed Critical General Electric Co
Priority to ES13784927.9T priority Critical patent/ES2609763T3/es
Priority to EP13784927.9A priority patent/EP2844755B1/en
Priority to CN201380022584.6A priority patent/CN104254776B/zh
Priority to JP2015510354A priority patent/JP6248095B2/ja
Priority to CA2870038A priority patent/CA2870038C/en
Priority to IN8223DEN2014 priority patent/IN2014DN08223A/en
Publication of WO2013165870A1 publication Critical patent/WO2013165870A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24—Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples

Definitions

  • the present disclosure generally relates to solid compositions for ambient extraction, stabilization, and preservation of nucleic acids from a biological sample in a dry format. Methods for extracting, collecting, preserving, and recovering nucleic acids from the solid compositions are also described.
  • a composition comprising a protein denaturant, a reducing agent, and a buffer is present in the dry solid matrix of this disclosure.
  • the composition may comprise one or more of each of the above-listed components.
  • the composition may optionally further comprise an ultraviolet (UV) inhibitor, a free-radical trap, an RNase inhibitor, a chelator, or any combination thereof.
  • UV ultraviolet
  • the skilled artisan will appreciate that numerous protein denaturants are known in the art and can be empirically selected for use in the compositions and methods described here.
  • X is an anion, which is a radical containing one or more formal negative charge(s).
  • Buffer as used herein includes, for example, 2-Amino-2- hydroxymethyl-propane-l,3-diol (Tris), 2-(N-morpholino)ethanesulfonic acid (MES), 3- (N-morpholino)propanesulfonic acid (MOPS), citrate buffers, 4-(2-hydroxyethyl)-l- piperazineethanesulfonic acid (HEPES), and phosphate buffers.
  • This list of potential buffers is for illustrative purposes only. The skilled artisan would recognize that the pH of the buffer selected for use in the compositions and methods disclosed herein is relevant. The pH of the buffer will typically be in the range of 3 to 8.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
PCT/US2013/038576 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids Ceased WO2013165870A1 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ES13784927.9T ES2609763T3 (es) 2012-04-30 2013-04-29 Métodos y composiciones para la extracción y el almacenamiento de ácidos nucleicos
EP13784927.9A EP2844755B1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids
CN201380022584.6A CN104254776B (zh) 2012-04-30 2013-04-29 提取和储存核酸的方法和组合物
JP2015510354A JP6248095B2 (ja) 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物
CA2870038A CA2870038C (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids
IN8223DEN2014 IN2014DN08223A (https=) 2012-04-30 2013-04-29

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/460,076 2012-04-30
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids

Publications (1)

Publication Number Publication Date
WO2013165870A1 true WO2013165870A1 (en) 2013-11-07

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Family Applications (1)

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PCT/US2013/038576 Ceased WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Country Status (8)

Country Link
US (1) US9044738B2 (https=)
EP (1) EP2844755B1 (https=)
JP (3) JP6248095B2 (https=)
CN (1) CN104254776B (https=)
CA (1) CA2870038C (https=)
ES (1) ES2609763T3 (https=)
IN (1) IN2014DN08223A (https=)
WO (1) WO2013165870A1 (https=)

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CN105961375A (zh) * 2016-07-13 2016-09-28 北京中科唯新生物医学研究所有限公司 一种唾液保存液及其制备方法和用途
CN107076764A (zh) * 2014-11-20 2017-08-18 通用电气医疗集团英国有限公司 检测稳定在固体载体材料上的痴呆和阿尔茨海默病相关生物标记
WO2017157650A1 (en) * 2016-03-18 2017-09-21 Hummingbird Diagnostics Gmbh Purification of rna fractions using a hydrophilic polymeric material
WO2021229066A1 (en) * 2020-05-14 2021-11-18 Bioecho Life Sciences Gmbh Isolation of nucleic acids at elevated temperatures
US11266337B2 (en) 2015-09-09 2022-03-08 Drawbridge Health, Inc. Systems, methods, and devices for sample collection, stabilization and preservation
EP4206323A4 (en) * 2020-08-28 2024-08-28 Kao Corporation Rna storage method

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TWI882519B (zh) 2017-01-10 2025-05-01 美商集聯健康有限公司 用於收集和儲存來自受試者之血液的裝置
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
US12264311B2 (en) * 2019-01-25 2025-04-01 Sekisui Chemical Co., Ltd. Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107076764A (zh) * 2014-11-20 2017-08-18 通用电气医疗集团英国有限公司 检测稳定在固体载体材料上的痴呆和阿尔茨海默病相关生物标记
EP3221704A4 (en) * 2014-11-20 2018-04-11 GE Healthcare UK Limited Detecting dementia and alzheimer's disease associated biomarkers stabilized on solid support materials
US11266337B2 (en) 2015-09-09 2022-03-08 Drawbridge Health, Inc. Systems, methods, and devices for sample collection, stabilization and preservation
WO2017157650A1 (en) * 2016-03-18 2017-09-21 Hummingbird Diagnostics Gmbh Purification of rna fractions using a hydrophilic polymeric material
AU2017233436B2 (en) * 2016-03-18 2022-11-24 Hummingbird Diagnostics Gmbh Purification of RNA fractions using a hydrophilic polymeric material
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CN105961375A (zh) * 2016-07-13 2016-09-28 北京中科唯新生物医学研究所有限公司 一种唾液保存液及其制备方法和用途
WO2021229066A1 (en) * 2020-05-14 2021-11-18 Bioecho Life Sciences Gmbh Isolation of nucleic acids at elevated temperatures
EP4206323A4 (en) * 2020-08-28 2024-08-28 Kao Corporation Rna storage method

Also Published As

Publication number Publication date
CN104254776B (zh) 2016-09-28
IN2014DN08223A (https=) 2015-05-15
ES2609763T3 (es) 2017-04-24
CA2870038A1 (en) 2013-11-07
US20130289265A1 (en) 2013-10-31
EP2844755A1 (en) 2015-03-11
JP2018068303A (ja) 2018-05-10
CA2870038C (en) 2021-08-03
EP2844755B1 (en) 2016-11-16
JP6248095B2 (ja) 2017-12-13
US9044738B2 (en) 2015-06-02
JP2015518569A (ja) 2015-07-02
CN104254776A (zh) 2014-12-31
JP2020092709A (ja) 2020-06-18
EP2844755A4 (en) 2015-03-11
JP6847365B2 (ja) 2021-03-24

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