JP6248095B2 - 核酸の抽出及び貯蔵のための方法及び組成物 - Google Patents

核酸の抽出及び貯蔵のための方法及び組成物 Download PDF

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JP6248095B2
JP6248095B2 JP2015510354A JP2015510354A JP6248095B2 JP 6248095 B2 JP6248095 B2 JP 6248095B2 JP 2015510354 A JP2015510354 A JP 2015510354A JP 2015510354 A JP2015510354 A JP 2015510354A JP 6248095 B2 JP6248095 B2 JP 6248095B2
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solid matrix
rna
nucleic acid
sample
acid
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JP2015518569A5 (https=
JP2015518569A (ja
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リ,ビン
ムーア,デイヴィッド・ロジャー
クヴァム,エリック・リーミング
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General Electric Co
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/24Naturally occurring macromolecular compounds, e.g. humic acids or their derivatives
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2015510354A 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物 Active JP6248095B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13/460,076 2012-04-30
US13/460,076 US9044738B2 (en) 2012-04-30 2012-04-30 Methods and compositions for extraction and storage of nucleic acids
PCT/US2013/038576 WO2013165870A1 (en) 2012-04-30 2013-04-29 Methods and compositions for extraction and storage of nucleic acids

Related Child Applications (1)

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JP2017225326A Division JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物

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JP2015518569A JP2015518569A (ja) 2015-07-02
JP2015518569A5 JP2015518569A5 (https=) 2016-06-09
JP6248095B2 true JP6248095B2 (ja) 2017-12-13

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JP2015510354A Active JP6248095B2 (ja) 2012-04-30 2013-04-29 核酸の抽出及び貯蔵のための方法及び組成物
JP2017225326A Active JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物
JP2020036905A Pending JP2020092709A (ja) 2012-04-30 2020-03-04 核酸の抽出及び貯蔵のための方法及び組成物

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JP2017225326A Active JP6847365B2 (ja) 2012-04-30 2017-11-24 核酸の抽出及び貯蔵のための方法及び組成物
JP2020036905A Pending JP2020092709A (ja) 2012-04-30 2020-03-04 核酸の抽出及び貯蔵のための方法及び組成物

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US (1) US9044738B2 (https=)
EP (1) EP2844755B1 (https=)
JP (3) JP6248095B2 (https=)
CN (1) CN104254776B (https=)
CA (1) CA2870038C (https=)
ES (1) ES2609763T3 (https=)
IN (1) IN2014DN08223A (https=)
WO (1) WO2013165870A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016527897A (ja) * 2013-08-16 2016-09-15 ゼネラル・エレクトリック・カンパニイ 核酸の抽出及び保存のための方法及び組成物

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9480966B2 (en) 2012-04-30 2016-11-01 General Electric Company Substrates and methods for collection, stabilization and elution of biomolecules
US10829291B2 (en) * 2012-06-20 2020-11-10 Thermo Fischer Scientific Baltics UAB Method to prevent silica-based column aging
ES2786373T3 (es) 2014-06-10 2020-10-09 Biomatrica Inc Estabilización de trombocitos a temperaturas ambiente
JP2018502281A (ja) * 2014-11-20 2018-01-25 ジーイー・ヘルスケア・ユーケイ・リミテッド 固体支持体材料に固定された認知症及びアルツハイマー病関連バイオマーカーの検出
GB201505516D0 (en) * 2015-03-31 2015-05-13 Ge Healthcare Uk Ltd Improvements in and relating to biological sample collectors and handling thereof
ES2818569T3 (es) 2015-09-09 2021-04-13 Drawbridge Health Inc Métodos para la recopilación, estabilización y conservación de muestras
US10000742B2 (en) * 2015-11-19 2018-06-19 General Electric Company Device and method of collection for RNA viruses
KR20250047404A (ko) 2015-12-08 2025-04-03 바이오매트리카 인코포레이티드 적혈구 침강 속도의 감소
AU2017233436B2 (en) * 2016-03-18 2022-11-24 Hummingbird Diagnostics Gmbh Purification of RNA fractions using a hydrophilic polymeric material
CN105961375A (zh) * 2016-07-13 2016-09-28 北京中科唯新生物医学研究所有限公司 一种唾液保存液及其制备方法和用途
US11166658B2 (en) * 2016-07-28 2021-11-09 Invitae Corporation Blood sampling system and method
EP3528950A4 (en) * 2016-10-24 2020-05-27 Biomatrica, INC. STABILIZATION OF NUCLEIC ACIDS ON PAPER
GB2590814B (en) 2017-01-10 2021-11-03 Drawbridge Health Inc Devices, systems, and methods for sample collection
CN109182332A (zh) * 2018-09-30 2019-01-11 浙江天杭生物科技股份有限公司 一种从临床样品中快速简便提取核酸的方法
SG11202104814XA (en) * 2019-01-25 2021-08-30 Sekisui Chemical Co Ltd Method for isolating nucleic acid, nucleic acid isolation kit, and inspection chip
JP2023526038A (ja) * 2020-05-14 2023-06-20 バイオエコー ライフ サイエンシズ ゲーエムベーハー 上昇させた温度での核酸の単離
KR102907458B1 (ko) 2020-08-28 2026-01-02 카오카부시키가이샤 Rna 의 보존 방법
KR20230171426A (ko) * 2021-03-19 2023-12-20 백톤 디킨슨 앤드 컴퍼니 병원체의 등온 증폭
GB202211645D0 (en) 2022-08-09 2022-09-21 Hemodx As Kit
SE548123C2 (en) * 2023-11-08 2026-03-31 Sequrna Ab Device for dry storage of biological samples, conserving RNA from degradation and use of such devices

Family Cites Families (59)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4540506A (en) 1983-04-15 1985-09-10 Genex Corporation Composition for cleaning drains clogged with deposits containing hair
CA1255197A (en) * 1984-09-24 1989-06-06 Joseph L. Orlik Leukocyte differentiation composition and method
US5173422A (en) 1986-08-22 1992-12-22 Miles Inc. Monoclonal antibodies specific for human glycoalbumin
US5756126A (en) 1991-05-29 1998-05-26 Flinders Technologies Pty. Ltd. Dry solid medium for storage and analysis of genetic material
US5807527A (en) 1991-05-29 1998-09-15 Flinders Technologies Pty. Ltd. Solid medium and method for DNA storage
US6627226B2 (en) 1988-10-05 2003-09-30 Whatman, Inc. Dry solid medium for storage and analysis of genetic material
DE96915910T1 (de) 1995-06-07 2004-04-22 Whatman Plc, Maidstone Trockenes festes medium zur lagerung und analyse von genetischem material
WO1997006256A2 (en) 1995-08-09 1997-02-20 Institut National De La Sante Et De La Recherche Medicale Isolated nucleic acid molecules useful as leukemia markers and in breast cancer prognosis
US5939259A (en) 1997-04-09 1999-08-17 Schleicher & Schuell, Inc. Methods and devices for collecting and storing clinical samples for genetic analysis
US6204375B1 (en) 1998-07-31 2001-03-20 Ambion, Inc. Methods and reagents for preserving RNA in cell and tissue samples
DE19836559A1 (de) 1998-08-12 2000-03-23 Antigen Gmbh Gefäß zur Entnahme von Blut
US20010039010A1 (en) 1998-09-03 2001-11-08 Leigh Alexander Burgoyne Sample collection medium incorporating material for sample visualization
EP1177281B1 (en) 1999-04-30 2009-01-21 Cyclops Genome Sciences Limited Isolation of nucleic acid
AU4353500A (en) 1999-04-30 2000-11-17 Whatman, Inc. Substrate including anionic detergent for purifying nucleic acid
DE60044977D1 (de) 1999-09-24 2010-10-28 Ambion Inc Cocktail von nukleaseinhibitoren
DE10006662A1 (de) 2000-02-15 2001-08-23 Antigen Produktions Gmbh Gefäß zur Nukleinsäureanalytik
US6602718B1 (en) 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
US7531362B2 (en) 2001-06-07 2009-05-12 Medmira Inc. Rapid diagnostic assay
US20070117173A1 (en) 2001-09-05 2007-05-24 Levison Peter R Stable storage of proteins
GB0129816D0 (en) * 2001-12-13 2002-01-30 The Technology Partnership Plc Testing device for chemical or biochemical analysis
US7482116B2 (en) 2002-06-07 2009-01-27 Dna Genotek Inc. Compositions and methods for obtaining nucleic acids from sputum
JP2005532072A (ja) 2002-07-10 2005-10-27 マサチューセッツ・インスティテュート・オブ・テクノロジー サンプルから核酸を単離するための装置および方法
DE10231659B4 (de) 2002-07-12 2006-01-19 Preanalytix Gmbh Zusammensetzung zum Binden von Nukleinsäure an eine Festphase
GB0217963D0 (en) 2002-08-02 2002-09-11 Cyclops Genome Sciences Ltd Purification of nucleic acids
US7267950B2 (en) 2003-05-01 2007-09-11 Veridex, Lcc Rapid extraction of RNA from cells and tissues
JP2007502218A (ja) * 2003-05-23 2007-02-08 ユィロス・パテント・アクチボラグ 親水性/疎水性表面
US7250270B2 (en) 2003-06-13 2007-07-31 Ambion, Inc. Methods and compositions for preparing tissue samples for RNA extraction
EP1641944B1 (en) 2003-07-09 2011-06-29 Genvault Corporation Room temperature elution of nucleic acids
JP3714940B2 (ja) 2003-11-07 2005-11-09 株式会社日立ハイテクノロジーズ Rna抽出方法、および生体材料の分析方法
US20060099567A1 (en) 2004-04-08 2006-05-11 Biomatrica, Inc. Integration of sample storage and sample management for life science
US20080176209A1 (en) 2004-04-08 2008-07-24 Biomatrica, Inc. Integration of sample storage and sample management for life science
DE602005026273D1 (de) 2004-04-09 2011-03-24 Vivebio Llc Vorrichtungen und verfahren für abnahme, lagerung und transport von biologischen proben
DE102004021780B4 (de) 2004-04-30 2008-10-02 Siemens Ag Verfahren und Anordnung zur DNA-Isolierung mit Trockenreagenzien
DE602005016402D1 (de) 2004-05-24 2009-10-15 Genvault Corp Stabile lagerung von protein und stabile lagerung von nukleinsäure in wiedergewinnbarer form
US7776530B2 (en) 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis
US20090208919A1 (en) 2005-01-21 2009-08-20 Argylla Technologies, Llp Particle matrix for storage of biomolecules
US8158357B2 (en) 2005-03-16 2012-04-17 Dna Genotek Inc. Compositions and method for storage of nucleic acid from bodily fluids
GB0505909D0 (en) 2005-03-23 2005-04-27 Univ Leeds Formulations
US20070015165A1 (en) 2005-07-13 2007-01-18 Sigma-Aldrich Co. Method for the isolation of RNA from biological sources
JP4699868B2 (ja) * 2005-11-04 2011-06-15 株式会社日立ハイテクノロジーズ 核酸精製方法及び核酸精製器具
EP1963526A4 (en) 2005-12-09 2009-11-18 Promega Corp PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US9458451B2 (en) * 2007-06-21 2016-10-04 Gen-Probe Incorporated Multi-channel optical measurement instrument
US20090053704A1 (en) 2007-08-24 2009-02-26 Natalia Novoradovskaya Stabilization of nucleic acids on solid supports
CN101878304B (zh) * 2007-11-30 2015-01-07 通用电气医疗集团生物科学公司 用于从单个样品中分离基因组dna、rna和蛋白质的方法
WO2009117167A1 (en) 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
US20100209957A1 (en) 2008-06-20 2010-08-19 Genvault Corporation Biosample storage devices and methods of use thereof
WO2010031007A2 (en) 2008-09-12 2010-03-18 Genvault Corporation Matrices and media for storage and stabilization of biomolecules
JP5766178B2 (ja) * 2009-03-24 2015-08-19 ザ・ユニバーシティ・オブ・シカゴThe University Of Chicago SlipChip装置および方法
EP2430195B1 (en) * 2009-05-11 2019-01-23 Biomatrica, INC. Compositions and methods for biological sample storage
CA2796729C (en) 2010-04-20 2020-06-23 Octapharma Ag Melezitose for stabilizing human blood plasma proteins
EP2388312A1 (en) 2010-05-17 2011-11-23 Curetis AG Universally applicable lysis buffer and processing methods for the lysis of bodily samples
EP2598660B1 (en) 2010-07-26 2017-03-15 Biomatrica, INC. Compositions for stabilizing dna, rna and proteins in blood and other biological samples during shipping and storage at ambient temperatures
WO2012075471A1 (en) 2010-12-04 2012-06-07 Hui-Yu Liu Rna stability enhancer
US20120152743A1 (en) 2010-12-17 2012-06-21 General Electric Company Method for electroeluting genetic material from dried samples
GB201103257D0 (en) 2011-02-25 2011-04-13 Ge Healthcare Uk Ltd Paper support and method of recovering biological material therefrom
GB2496122A (en) 2011-10-31 2013-05-08 Ge Healthcare Bio Sciences Ab Biological sample preservation on paper
CN103173432B (zh) * 2011-12-22 2020-08-04 通用电气公司 分离核酸的方法及装置
US9040675B2 (en) 2012-04-30 2015-05-26 General Electric Company Formulations for nucleic acid stabilization on solid substrates

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2016527897A (ja) * 2013-08-16 2016-09-15 ゼネラル・エレクトリック・カンパニイ 核酸の抽出及び保存のための方法及び組成物

Also Published As

Publication number Publication date
CN104254776A (zh) 2014-12-31
JP2020092709A (ja) 2020-06-18
JP2018068303A (ja) 2018-05-10
CA2870038C (en) 2021-08-03
CA2870038A1 (en) 2013-11-07
CN104254776B (zh) 2016-09-28
EP2844755A1 (en) 2015-03-11
JP6847365B2 (ja) 2021-03-24
EP2844755B1 (en) 2016-11-16
IN2014DN08223A (https=) 2015-05-15
JP2015518569A (ja) 2015-07-02
US9044738B2 (en) 2015-06-02
US20130289265A1 (en) 2013-10-31
EP2844755A4 (en) 2015-03-11
ES2609763T3 (es) 2017-04-24
WO2013165870A1 (en) 2013-11-07

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