CN104250645A - 一种rna片段及其制备方法和用途 - Google Patents
一种rna片段及其制备方法和用途 Download PDFInfo
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- CN104250645A CN104250645A CN201310263324.XA CN201310263324A CN104250645A CN 104250645 A CN104250645 A CN 104250645A CN 201310263324 A CN201310263324 A CN 201310263324A CN 104250645 A CN104250645 A CN 104250645A
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- rna fragment
- rna
- triphosphoric acid
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (10)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310263324.XA CN104250645B (zh) | 2013-06-27 | 2013-06-27 | 一种rna片段及其制备方法和用途 |
PCT/CN2014/080069 WO2014206219A1 (zh) | 2013-06-27 | 2014-06-17 | 一种rna片段及其制备方法和用途 |
HK15104327.9A HK1204001A1 (zh) | 2013-06-27 | 2015-05-06 | 種 片段及其製備方法和用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310263324.XA CN104250645B (zh) | 2013-06-27 | 2013-06-27 | 一种rna片段及其制备方法和用途 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104250645A true CN104250645A (zh) | 2014-12-31 |
CN104250645B CN104250645B (zh) | 2017-05-03 |
Family
ID=52141030
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310263324.XA Active CN104250645B (zh) | 2013-06-27 | 2013-06-27 | 一种rna片段及其制备方法和用途 |
Country Status (3)
Country | Link |
---|---|
CN (1) | CN104250645B (zh) |
HK (1) | HK1204001A1 (zh) |
WO (1) | WO2014206219A1 (zh) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1611606A (zh) * | 2003-10-30 | 2005-05-04 | 封江南 | 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法 |
CN102618664A (zh) * | 2012-05-03 | 2012-08-01 | 武汉大学 | 一种miRNA检测探针和可视化检测miRNA的方法 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002360256A (ja) * | 2001-06-04 | 2002-12-17 | Inst Of Physical & Chemical Res | Rnaプローブ作成方法、標的核酸の検出方法及びrnaプローブ作成用キット |
CN1162547C (zh) * | 2002-08-30 | 2004-08-18 | 山东大学 | 靶向肝癌的乙肝病毒反义rna表达载体及其构建方法与用途 |
CN101892226A (zh) * | 2010-03-11 | 2010-11-24 | 新乡医学院 | 一种抑制人脂肪细胞ACC基因表达的siRNA及其表达载体和应用 |
-
2013
- 2013-06-27 CN CN201310263324.XA patent/CN104250645B/zh active Active
-
2014
- 2014-06-17 WO PCT/CN2014/080069 patent/WO2014206219A1/zh active Application Filing
-
2015
- 2015-05-06 HK HK15104327.9A patent/HK1204001A1/zh unknown
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1611606A (zh) * | 2003-10-30 | 2005-05-04 | 封江南 | 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法 |
CN102618664A (zh) * | 2012-05-03 | 2012-08-01 | 武汉大学 | 一种miRNA检测探针和可视化检测miRNA的方法 |
Non-Patent Citations (6)
Title |
---|
MICHAEL MZ ET AL.: "MIMAT0000617", 《MIRBASE》 * |
MOURELATOS Z ET AL.: "MIMAT0000095", 《MIRBASE》 * |
PRISCILLA HIU-MEI TOO ET AL.: "Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs", 《NUCLEIC ACIDS RESEARCH》 * |
刘霞等: "子宫内膜腺癌组织中miRNA的差异表达", 《中华医学杂志》 * |
唐向辉等: "体外制备RNA探针——一种极有希望的从质粒DNA合成单链RNA新技术", 《医学分子生物学杂志》 * |
李淑婷等: "地高辛精标记酪氨酸羟化酶RNA探针的制备研究", 《中国组织化学与细胞化学杂志》 * |
Also Published As
Publication number | Publication date |
---|---|
HK1204001A1 (zh) | 2015-11-06 |
WO2014206219A1 (zh) | 2014-12-31 |
CN104250645B (zh) | 2017-05-03 |
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