CN104250645B - 一种rna片段及其制备方法和用途 - Google Patents
一种rna片段及其制备方法和用途 Download PDFInfo
- Publication number
- CN104250645B CN104250645B CN201310263324.XA CN201310263324A CN104250645B CN 104250645 B CN104250645 B CN 104250645B CN 201310263324 A CN201310263324 A CN 201310263324A CN 104250645 B CN104250645 B CN 104250645B
- Authority
- CN
- China
- Prior art keywords
- fragment
- rna
- modification
- triphosphoric acid
- transcription
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering N.A.
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2330/00—Production
- C12N2330/50—Biochemical production, i.e. in a transformed host cell
Abstract
Description
Claims (7)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310263324.XA CN104250645B (zh) | 2013-06-27 | 2013-06-27 | 一种rna片段及其制备方法和用途 |
PCT/CN2014/080069 WO2014206219A1 (zh) | 2013-06-27 | 2014-06-17 | 一种rna片段及其制备方法和用途 |
HK15104327.9A HK1204001A1 (zh) | 2013-06-27 | 2015-05-06 | 種 片段及其製備方法和用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201310263324.XA CN104250645B (zh) | 2013-06-27 | 2013-06-27 | 一种rna片段及其制备方法和用途 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104250645A CN104250645A (zh) | 2014-12-31 |
CN104250645B true CN104250645B (zh) | 2017-05-03 |
Family
ID=52141030
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201310263324.XA Active CN104250645B (zh) | 2013-06-27 | 2013-06-27 | 一种rna片段及其制备方法和用途 |
Country Status (3)
Country | Link |
---|---|
CN (1) | CN104250645B (zh) |
HK (1) | HK1204001A1 (zh) |
WO (1) | WO2014206219A1 (zh) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1611606A (zh) * | 2003-10-30 | 2005-05-04 | 封江南 | 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2002360256A (ja) * | 2001-06-04 | 2002-12-17 | Inst Of Physical & Chemical Res | Rnaプローブ作成方法、標的核酸の検出方法及びrnaプローブ作成用キット |
CN1162547C (zh) * | 2002-08-30 | 2004-08-18 | 山东大学 | 靶向肝癌的乙肝病毒反义rna表达载体及其构建方法与用途 |
CN101892226A (zh) * | 2010-03-11 | 2010-11-24 | 新乡医学院 | 一种抑制人脂肪细胞ACC基因表达的siRNA及其表达载体和应用 |
CN102618664B (zh) * | 2012-05-03 | 2014-01-01 | 武汉大学 | 一种miRNA检测探针和可视化检测miRNA的方法 |
-
2013
- 2013-06-27 CN CN201310263324.XA patent/CN104250645B/zh active Active
-
2014
- 2014-06-17 WO PCT/CN2014/080069 patent/WO2014206219A1/zh active Application Filing
-
2015
- 2015-05-06 HK HK15104327.9A patent/HK1204001A1/zh unknown
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1611606A (zh) * | 2003-10-30 | 2005-05-04 | 封江南 | 单一载体编码多个发夹结构小干扰性rna表达系统及其构建方法 |
Non-Patent Citations (2)
Title |
---|
Engineering Nt.BtsCI and Nb.BtsCI nicking enzymes and applications in generating long overhangs;Priscilla Hiu-Mei Too et al.;《Nucleic Acids Research》;20091202;第38卷(第4期);第1295页左栏第3段,图1A * |
体外制备RNA探针——一种极有希望的从质粒DNA合成单链RNA新技术;唐向辉等;《医学分子生物学杂志》;19861231(第3期);第145页左栏第3段 * |
Also Published As
Publication number | Publication date |
---|---|
CN104250645A (zh) | 2014-12-31 |
WO2014206219A1 (zh) | 2014-12-31 |
HK1204001A1 (zh) | 2015-11-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6632596B2 (ja) | 分子計数のための組成物およびキット | |
CN105934523A (zh) | 核酸的多重检测 | |
CN1898395B (zh) | 基于引物的核酸的多重扩增的方法和试剂盒 | |
CN107002292B (zh) | 一种核酸的双接头单链环状文库的构建方法和试剂 | |
CN103114131B (zh) | 一种引物中部序列干扰pcr技术 | |
CN105899680A (zh) | 核酸探针和检测基因组片段的方法 | |
CN107446919B (zh) | 一种恒温条件下合成核酸的方法及试剂盒 | |
JP6739339B2 (ja) | カバーされた配列変換dnaおよび検出方法 | |
CN106544409A (zh) | 用于核酸指数式扩增的切口和延长扩增反应 | |
CN109689888A (zh) | 无细胞核酸标准品及其用途 | |
US11591646B2 (en) | Small RNA detection method based on small RNA primed xenosensor module amplification | |
JP2010514452A (ja) | ヘテロ二重鎖による濃縮 | |
CN106536735A (zh) | 分析dna样品的探针集合和使用所述探针集合的方法 | |
US20060003337A1 (en) | Detection of small RNAS | |
CN107109698A (zh) | Rna stitch测序:用于直接映射细胞中rna:rna相互作用的测定 | |
US9752180B2 (en) | Methods and compositions for amplification and detection of MicroRNAs | |
CN114958997A (zh) | 用于检测伴侣基因的方法 | |
CN116406428A (zh) | 用于使用酶促核酸延伸进行原位单细胞分析的组合物和方法 | |
CN102933720B (zh) | 用于生成具有单链悬突的双链核酸的方法 | |
WO2017215517A1 (zh) | 测序文库构建中5'和3'接头连接副产物的去除方法 | |
CN106255763A (zh) | 核酸扩增 | |
CN109072296A (zh) | 利用核酸酶保护进行直接标靶测序的方法 | |
US10889853B2 (en) | RNA microarray for detecting interaction between protein and RNA containing a higher-order structure | |
AU2017312947A1 (en) | High throughput oil-emulsion synthesis of bowtie barcodes for paired mrna capture and sequencing from individual cells | |
EA013373B1 (ru) | Определение нуклеиновой кислоты |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
ASS | Succession or assignment of patent right |
Free format text: FORMER OWNER: WEN TING Effective date: 20150420 Owner name: KUNSHAN PENGJI KAIFENG BIOLOGICAL SCIENCE + TECHNO Free format text: FORMER OWNER: PENG CHANGGENG Effective date: 20150420 |
|
C41 | Transfer of patent application or patent right or utility model | ||
COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 417706 LOUDI, HUNAN PROVINCE TO: 215300 SUZHOU, JIANGSU PROVINCE |
|
TA01 | Transfer of patent application right |
Effective date of registration: 20150420 Address after: 215300 No. 209 Feng Feng Road, hi tech Zone, Jiangsu, Kunshan Applicant after: KUNSHAN PENGJI KAIFENG BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. Address before: 417706, Dahe County, Loudi Town, Shuangfeng County, Hunan, China Applicant before: Peng Changgeng Applicant before: Wen Ting |
|
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1204001 Country of ref document: HK |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: WD Ref document number: 1204001 Country of ref document: HK |
|
TR01 | Transfer of patent right |
Effective date of registration: 20221102 Address after: 202H, A2/F, Jinbolong Industrial Plant, No.1 Huayun Road, Yucui Community, Longhua Street, Longhua District, Shenzhen, Guangdong 518000 Patentee after: Shenzhen Yifu Technology Co.,Ltd. Address before: No. 209, Chenfeng Road, High tech Zone, Kunshan City, Jiangsu Province 215300 Patentee before: KUNSHAN PENGJI KAIFENG BIOLOGICAL SCIENCE & TECHNOLOGY Co.,Ltd. |
|
TR01 | Transfer of patent right |