CN104233365B - The electrochemical reactor and preparation method of oligosaccharides are prepared from marine organisms functional sugar - Google Patents
The electrochemical reactor and preparation method of oligosaccharides are prepared from marine organisms functional sugar Download PDFInfo
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- CN104233365B CN104233365B CN201410525615.6A CN201410525615A CN104233365B CN 104233365 B CN104233365 B CN 104233365B CN 201410525615 A CN201410525615 A CN 201410525615A CN 104233365 B CN104233365 B CN 104233365B
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Abstract
The invention provides electrochemical reactor and preparation method that oligosaccharides is prepared from marine organisms functional sugar, belong to electrolysis field.It solves the technical problems such as the existing technology that oligosaccharides is not obtained from marine organisms functional sugar by electrochemical reaction.This prepares the electrochemical reactor of oligosaccharides from marine organisms functional sugar, including the reactive tank with cavity, two pieces of dividing plates are fixed with reactive tank, cavity is separated into three chambers by two pieces of dividing plates, three chambers are reaction chamber and the flow cavity for being located at reaction chamber both sides respectively, the opening of connection reaction chamber and flow cavity is provided with two pieces of dividing plates, pole plate component is provided with reaction chamber.The electrochemical reactor has the advantages that to be easily achieved integrated control with optimization.The preparation method of oligosaccharides, including A, charging are prepared from marine organisms functional sugar；B, reaction；C, discharging；D, separation and collection.The preparation method has the advantages that oligosaccharides can be extracted from marine organisms functional sugar.
Description
Technical field
The invention belongs to be electrolysed field, it is related to a kind of electrochemical reactor, particularly one kind is from marine organisms functional sugar
Prepare the electrochemical reactor of oligosaccharides；A kind of preparation method the invention further relates to prepare oligosaccharides in functional sugar from marine organisms,
Belong to organic chemistry filed.
Background technology
Marine organisms are widely distributed in ocean, therefore resource is enriched very much, and ripe technology does not pass through electricity at present
Chemical reaction oligosaccharides is obtained from marine organisms functional sugar.
Simultaneously current electrochemical reactor controllability angle, it is impossible to meet and oligosaccharides is obtained from marine organisms functional sugar
It is required that, such as high-efficiency electrochemical reactor disclosed in Chinese patent literature【CN101654294A】, including cell body, it is parallel in cell body
Arrange some pole plates.Solution flowing in such electrochemical reactor between pole plate has some setbacks very much, causes between each pole plate
Response situation differ, there is reaction solution and react insufficient, uneven situation, cause uncontrollable reaction after the reaction
The concentration of product in liquid, controllable degree is relatively low.
The content of the invention
The purpose of the present invention is directed to existing technology and there is above mentioned problem, it is proposed that one kind is from marine organisms functional sugar
The electrochemical reactor of oligosaccharides is prepared, present invention solves the technical problem that being the controllable degree for improving electrochemical reaction.
The invention allows for the preparation method that oligosaccharides is prepared in a kind of functional sugar from marine organisms, it is another that the invention is solved
One technical problem is to prepare oligosaccharides from marine organisms functional sugar.
First technical problem that the present invention is solved can be realized by following technical proposal：
The electrochemical reactor of oligosaccharides, including the reactive tank with cavity are prepared from marine organisms functional sugar, it is described anti-
Answer and be fixed with groove two pieces of dividing plates, cavity is separated into three chambers by two pieces of dividing plates, three chambers are reaction chamber and are located at respectively
The flow cavity of reaction chamber both sides, is provided with the opening of connection reaction chamber and flow cavity, in the reaction chamber on two pieces of dividing plates
Be provided with pole plate component, pole plate component include two pieces be arranged in parallel in pole plate and positioned at two pieces while pole plate between some interior pole
Plate, is provided with the mounting hole for installing electrode on two pieces of side pole plates, the internal polar plate is parallel with side pole plate, the internal polar plate and
Side pole plate and divider upright, the internal polar plate and be provided between pole plate by internal polar plate and while pole plate be positioned at determining for reaction chamber
Bit architecture.
By in the solution input cavity for needing reaction, solution is evenly distributed in reaction chamber and flow cavity, is connect in reaction
Lead to the electrode being connected on two side pole plates, solution reacts generation oligosaccharides between side pole plate and internal polar plate, and now solution is continuous
Flowed in reaction chamber and flow cavity, while also being flowed between side pole plate and internal polar plate and internal polar plate so that the widow of generation
Sugar can be uniformly dispersed in the solution in cavity, and the solution between side pole plate and internal polar plate and internal polar plate is unlikely to oligosaccharides occur
The situation of uneven concentration, makes reaction have extremely strong selectivity, prevents the side reaction that may occur, and increases the controllable of reaction
Property；The reaction efficiency that solution between side pole plate and internal polar plate and internal polar plate is consistent, enables solution to react abundant, increases
Reaction efficiency.
In the above-mentioned electrochemical reactor for preparing oligosaccharides from marine organisms functional sugar, the location structure includes exhausted
The locating rod that edge material is made, the locating rod is parallel with dividing plate, and the two ends of the locating rod are supported with the cell wall of reactive tank respectively
Lean on, location hole, the pore size of the location hole and the diameter phase of locating rod are provided with the internal polar plate and side pole plate
Together, the locating rod is through the location hole on each internal polar plate and side pole plate, and the locating rod is provided with some positioning sleeves, described
Positioning sleeve is located between internal polar plate and side pole plate and adjacent internal polar plate respectively.Locating rod adopts made of plastic, plays insulation
Effect；Locating rod links together internal polar plate and side pole plate so that internal polar plate remains parallel with side pole plate, while preventing
Internal polar plate and side pole plate are inclined；Positioning sleeve can fix gap length between internal polar plate and side pole plate and adjacent internal polar plate, while
The positioning sleeve of different length can be changed to adjust between internal polar plate and side pole plate and adjacent internal polar plate according to actual production demand
Gap length；The cell wall of fix bar and reactive tank is against making internal polar plate and side pole plate be fixed in reaction chamber, it is to avoid in input or
There is displacement when pouring out solution, it is ensured that the uniformity of each electrochemical reaction, it is ensured that the controllability of reaction, and can realize continuous raw
Produce.
In the above-mentioned electrochemical reactor for preparing oligosaccharides from marine organisms functional sugar, the locating rod passes side pole
The two ends of plate are equipped with fixed cover, two pieces of side pole plates respectively with fixed cover against, the side of the fixed cover offers fixing hole,
Mounting hole corresponding with fixing hole is provided with locating rod, fixed cover is fixed on positioning by retaining pin through fixing hole and mounting hole
On bar.Positioned at locating rod two ends fixed cover by internal polar plate and while pole plate and positioned at internal polar plate and while pole plate and adjacent internal polar plate
Between positioning sleeve clamp so that closely connected between internal polar plate, side pole plate and positioning sleeve, between internal polar plate and internal polar plate with
Side pole plate is not in skew, carries out electrochemical reaction stabilization, so as to increase the controllability of reaction.
In the above-mentioned electrochemical reactor for preparing oligosaccharides from marine organisms functional sugar, the locating rod has three
Line successively is provided between three location holes, three location holes on root, internal polar plate and side pole plate and forms isosceles triangle, three fixed
Position bar is each passed through corresponding location hole on internal polar plate and side pole plate.The structure makes internal polar plate and side pole plate be in the interior pole with entirety
Between plate and internal polar plate and side pole plate are not in skew, carry out electrochemical reaction stabilization, so as to increase the controllable of reaction
Property.
In the above-mentioned electrochemical reactor for preparing oligosaccharides from marine organisms functional sugar, the mounting hole offers side
The middle part of the upper surface of pole plate, internal thread is provided with the hole wall of mounting hole.Electrode thread is connected on mounting hole, the position of mounting hole
Put the installation for facilitating electrode, the structure makes Potential Distributing on the pole plate of side uniform, make side pole plate and internal polar plate and internal polar plate it
Between solution reaction be consistent, increase reaction controllability.
In the above-mentioned electrochemical reactor for preparing oligosaccharides from marine organisms functional sugar, two pieces of internal polar plates and side
Gap is respectively provided with the opening being connected on two pieces of dividing plates between the gap of pole plate and adjacent internal polar plate.The structure can be anti-
It is seasonable to ensure to be flowed freely in cavity positioned at the solution in gap between internal polar plate and side pole plate and adjacent internal polar plate so that
The oligosaccharides of generation can be uniformly dispersed in the solution in cavity；Solution between side pole plate and internal polar plate and internal polar plate keeps
Consistent reaction efficiency, increases the controllability of reaction.
Second technical problem that the present invention is solved can be realized by following technical proposal：
The preparation method of oligosaccharides is prepared from marine organisms functional sugar, is comprised the following steps：
A, charging：The appropriate mixed solution containing marine organisms functional sugar is delivered to the anti-of electrochemical reactor from storage tank
Answer in groove, the amount of the shitosan sodium chloride mixed solution of input judges according to the liquid level gauge on electrochemical reactor；
B, reaction：Electrode on two side pole plates on electrochemical reactor is connected with constant-current supply, to electrochemical reaction
The mixed solution containing marine organisms functional sugar in device carries out electrochemical degradation, and the reaction time is T；
C, discharging：The reaction product solution containing oligosaccharides and monose for completing will be reacted and be delivered to storage from electrochemical reactor
Storage in flow container, and in real-time detection reaction product solution oligosaccharides concentration；
D, separation and collection：Reaction product solution is delivered to chromatographic column from fluid reservoir, chromatographic column has some, chromatography
It is connected in series in successively between post, reaction product solution passes sequentially through some chromatographic columns, and reaction product solution is in every chromatographic column
Absorption oligosaccharides, wash-out monose, wash-out oligosaccharides are inside carried out successively and collect the oligosaccharides being eluted out.
Mixed solution containing marine organisms functional sugar is shitosan sodium chloride mixed solution, is input into appropriate mixed solution energy
Electrochemical reactor efficiency is maximized, the mixed solution electrochemistry containing marine organisms functional sugar is dropped by electrochemical reactor
Solution produces the reaction product solution with oligosaccharides and monose, then oligosaccharides is separated and collected by way of chromatographic isolation,
So as to prepare oligosaccharides in the mixed solution containing marine organisms functional sugar；The chromatographic column of series winding is successively continuously to product
Solution carries out chromatographic isolation, can continuously collect oligosaccharides, efficiency high.
In the above-mentioned preparation method for preparing oligosaccharides from marine organisms functional sugar, in step C, the oligosaccharides of real-time detection
Concentration data transfer to PID controller, with default data be compared data by PID controller, and by comparative result
Transmit to PLC, by PLC coordination electrode and constant-current supply Connection Time, so as to control electrochemical reaction
The reaction time T of device.What the concentration energy direct reaction of oligosaccharides went out in electrochemical reactor in fluid reservoir reaction product solution is anti-
Answer situation, detect oligosaccharides concentration it is relatively low when PID controller be signaled to PLC, PLC control increases electricity
Pole and constant-current supply Connection Time, increase reaction time T and cause that the mixed solution containing marine organisms functional sugar fully reacts；Or
The size in gap between internal polar plate and side pole plate and adjacent internal polar plate in electrochemical reactor is adjusted, so as to accelerate or slow down
The speed of reaction causes that mixed solution fully reacts.
In the above-mentioned preparation method for preparing oligosaccharides from marine organisms functional sugar, by poly- by weak acid in step D
Acrylic type resins adsorb oligosaccharides；Monose is eluted by deionized water；By hydrochloric acid will adsorb oligosaccharides eluted out, salt
The concentration of acid is 0.07mol/l~0.13mol/l.Weak acid polyacrylic acid type resin is that 6 or so oligosaccharides has to the degree of polymerization
Stronger suction-operated, to the other materials in monose and reaction product solution then without suction-operated, therefore is gathered using weak acid
Acrylic type resins adsorb oligosaccharides efficiency high；Can be eluted by hydrochloric acid by the oligosaccharides of weak acid polyacrylic acid type resin adsorption
Come；Monose wash-out is first eluted into the purity that oligosaccharides improves collection oligosaccharides again.
In the above-mentioned preparation method for preparing oligosaccharides from marine organisms functional sugar, in step D after oligosaccharides has been collected
Chromatographic column is rinsed to neutrality by deionized water.The step enables that chromatographic column is circulated and is continuously separated oligosaccharides.
Compared with prior art, this electrochemical reactor that oligosaccharides is prepared from marine organisms functional sugar has and causes to produce
Oligosaccharides can be uniformly dispersed in the solution in cavity, be consistent the solution between side pole plate and internal polar plate and internal polar plate
Reaction efficiency, solution is reacted abundant, increased reaction efficiency；Regulation internal polar plate and side pole plate and adjacent internal polar plate it
Between the adjustable nodal potential of gap length, or by control electric current parameter, make reaction that there is extremely strong selectivity, preventing may generation
Side reaction, functional group, the construction unit of catabolite do not change substantially；This electrochemical reactor product is small simultaneously, operate letter
Single, yield is high, without or seldom produce the controllable degree and automatic control level of pollution whole process all higher, it is easy to accomplish collect
Into control and the advantage for optimizing.
This preparation method that oligosaccharides is prepared from marine organisms functional sugar has can extract widow from marine organisms functional sugar
The advantage of sugar.
Brief description of the drawings
Fig. 1 is the top cross sectional view of this electrochemical reactor.
Fig. 2 is the cross section structure diagram of A-A in Fig. 1.
Fig. 3 is the cross section structure diagram of B-B in Fig. 1.
Fig. 4 is the mplifying structure schematic diagram in C portions in Fig. 1.
In figure, 1, reactive tank；1a, reaction chamber；1b, flow cavity；2nd, dividing plate；2a, opening；3rd, side pole plate；3a, installation
Hole；4th, internal polar plate；5th, locating rod；51st, positioning sleeve；52nd, fixed cover；53rd, retaining pin；6th, liquid level gauge；7th, silk seat.
Specific embodiment
The following is specific embodiment of the invention and with reference to accompanying drawing, technical scheme is further described,
But the present invention is not limited to these embodiments.
Embodiment one
The electrochemical reactor of oligosaccharides is prepared from marine organisms functional sugar, as shown in Figure 1 to Figure 3, including with cavity
Reactive tank 1, is fixed with two pieces of dividing plates 2 in reactive tank 1, cavity is separated into three chambers by two pieces of dividing plates 2, and three chambers are anti-
Answer chamber 1a and respectively positioned at the flow cavity 1b of reaction chamber 1a both sides, connection reaction chamber 1a and flow cavity 1b is provided with two pieces of dividing plates 2
Opening 2a, be provided with pole plate component in reaction chamber 1a, pole plate component includes two pieces of side pole plates 3 be arrangeding in parallel and positioned at two pieces
Some internal polar plates 4 between side pole plate 3, internal polar plate 4 has seven pieces in the present embodiment one；Be provided with two pieces of side pole plates 3 for
The mounting hole 3a of electrode is installed, mounting hole 3a offers the middle part of the upper surface of side pole plate 3, is provided with the hole wall of mounting hole 3a interior
Screw thread, electrode is made of conventional Pb plates and graphite, and pollution will not be produced to reaction solution.Internal polar plate 4 is parallel with side pole plate 3,
Internal polar plate 4 and side pole plate 3 are vertical with dividing plate 2, between two pieces of internal polar plates 4 and the gap of side pole plate 3 and adjacent internal polar plate 4 between
Gap is respectively provided with the opening 2a being connected on two pieces of dividing plates 2.Internal polar plate 4 and be provided between pole plate 3 by internal polar plate 4 and while
Pole plate 3 is positioned at the location structure of reaction chamber 1a.
As shown in Figures 1 to 4, location structure includes adopting made of plastic playing a part of insulation locating rod 5, locating rod 5
Parallel with dividing plate 2, the two ends of locating rod 5 on internal polar plate 4 and side pole plate 3 respectively with the cell wall of reactive tank 1 against being provided with positioning
Hole, the pore size of location hole is identical with the diameter of locating rod 5.Preferably, locating rod 5 has three in the present embodiment one
Line successively is provided between three location holes, three location holes on root, internal polar plate 4 and side pole plate 3 and forms isosceles triangle, three
Locating rod 5 is each passed through corresponding location hole on internal polar plate 4 and side pole plate 3.Locating rod 5 is provided with eight positioning sleeves 51, positioning
Set 51 is located between internal polar plate 4 and side pole plate 3 and adjacent internal polar plate 4 respectively, and positioning sleeve 51 can fix internal polar plate 4 and side pole plate
Gap length between 3 and adjacent internal polar plate 4, while the positioning sleeve 51 of different length can be changed to adjust according to actual production demand
Section internal polar plate 4 and gap length between side pole plate 3 and adjacent internal polar plate 4.The two ends that locating rod 5 passes side pole plate 3 are equipped with
Fixed cover 52, two pieces of side pole plates 3 are respectively with fixed cover 52 against the side of fixed cover 52 offers fixing hole, is opened in locating rod 5
There is mounting hole 3a corresponding with fixing hole, fixed cover 52 is fixed on locating rod by retaining pin 53 through fixing hole and mounting hole 3a
On 5.
As depicted in figs. 1 and 2, liquid level gauge 6, inlet and the reactive tank 1 of liquid level gauge 6 are fixed on the lateral wall of reactive tank 1
Groove bottom flush, the upper end of liquid level gauge 6 is higher than reactive tank 1, and liquid level gauge 6 is made of transparent plastic, can be by observing liquid level
The height of liquid level judges the height of solution in reactive tank 1 in meter 6；Silk seat 7 is further fixed on the lateral wall of reactive tank 1, silk seat 7 is used
In the both positive and negative polarity for installing power supply.
This prepares the preparation method of oligosaccharides from marine organisms functional sugar, comprises the following steps：
A, charging：The appropriate mixed solution containing marine organisms functional sugar is delivered to the anti-of electrochemical reactor from storage tank
Answer in groove 1, the mixed solution containing marine organisms functional sugar is shitosan sodium chloride mixed solution, the shitosan sodium chloride of input is mixed
The amount for closing solution judges according to the liquid level gauge 6 on electrochemical reactor；
B, reaction：Electrode on two side pole plates 3 on electrochemical reactor is connected with constant-current supply, to electrochemical reaction
The mixed solution containing marine organisms functional sugar in device carries out electrochemical degradation, internal polar plate 4 and side in regulation electrochemical reactor
The size in gap between pole plate 3 and adjacent internal polar plate 4, so as to accelerate or slow down the speed of reaction come so that mixed solution fills
Divide reaction, the reaction time is T；
C, discharging：The reaction product solution containing oligosaccharides and monose for completing will be reacted and be delivered to storage from electrochemical reactor
Storage in flow container, and in real-time detection reaction product solution oligosaccharides concentration, the data transfer of the concentration of the oligosaccharides of real-time detection
To PID controller, with default data be compared data by PID controller, and comparative result is transmitted to PLC
On, by PLC coordination electrode and constant-current supply Connection Time, so that the reaction time T of electrochemical reactor is controlled, this
Reaction time T's is preferably two hours in embodiment one.
D, separation and collection：Reaction product solution is delivered to chromatographic column from fluid reservoir, chromatographic column has some, this reality
Chromatographic column has eight in applying example one, is connected in series in successively between eight chromatographic columns, and reaction product solution passes sequentially through eight layers
Analysis post, reaction product solution carries out absorption oligosaccharides, wash-out monose, wash-out oligosaccharides and collects wash-out successively in every chromatographic column
Oligosaccharides out；Chromatographic column is rinsed to neutrality by deionized water after oligosaccharides has been collected.
By adsorbing oligosaccharides by weak acid polyacrylic acid type resin in step D；Monose is eluted by deionized water；It is logical
Out, the concentration of hydrochloric acid is 0.1mol/l to the oligosaccharides eluted that persalt will be adsorbed.Weak acid polyacrylic acid type resin is to the degree of polymerization
Oligosaccharides for 6 or so has stronger suction-operated, and work is not adsorbed then to the other materials in monose and reaction product solution
With, therefore use weak acid polyacrylic acid type resin adsorption oligosaccharides efficiency high；By the widow of weak acid polyacrylic acid type resin adsorption
Sugar can be eluted out by hydrochloric acid；Monose wash-out is first eluted into the purity that oligosaccharides improves collection oligosaccharides again.
The appropriate mixed solution of input enters in the cavity of reactive tank 1, can maximize electrochemical reactor efficiency, mixing
Solution is evenly distributed in reaction chamber 1a and flow cavity 1b, the electrode being connected on two side pole plates 3 is connected in reaction, containing sea
The mixed solution of foreign biological function sugar reacts the reaction product solution for producing oligosaccharides and monose between side pole plate 3 and internal polar plate 4,
Solution constantly flows in reaction chamber 1a and flow cavity 1b, while also being flowed between side pole plate 3 and internal polar plate 4 and internal polar plate 4
It is dynamic so that the oligosaccharides of generation can be uniformly dispersed in the solution in cavity, between side pole plate 3 and internal polar plate 4 and internal polar plate 4
Solution is unlikely to the uneven situation of oligosaccharide concentration occur, protects the solution between side pole plate 3 and internal polar plate 4 and internal polar plate 4
Consistent reaction efficiency is held, solution is reacted abundant；Oligosaccharides is separated and collected by way of chromatographic isolation, so that
Oligosaccharides is prepared in the mixed solution containing marine organisms functional sugar；The chromatographic column of series winding is successively continuously to reaction product solution
Chromatographic isolation is carried out, oligosaccharides, efficiency high can be continuously collected.
Embodiment two
The present embodiment is essentially identical with the structure and principle of embodiment one, and different place is：Location structure includes
Location-plate, is provided with some slots on location-plate, slot spaced set, and the lower end of side pole plate and internal polar plate is connected to slot
Interior, location-plate is fixed on reactive tank in the groove bottom of reaction chamber.
Embodiment three
The present embodiment is essentially identical with the structure and principle of embodiment one, and different place is：Pass through in step D
The oligosaccharides eluted that the hydrochloric acid of 0.07mol/l will be adsorbed is out.
Example IV
The present embodiment is essentially identical with the structure and principle of embodiment one, and different place is：Pass through in step D
The oligosaccharides eluted that the hydrochloric acid of 0.13mol/l will be adsorbed is out.
Specific embodiment described herein is only to the spiritual explanation for example of the present invention.Technology neck belonging to of the invention
The technical staff in domain can be made various modifications or supplement to described specific embodiment or be replaced using similar mode
Generation, but without departing from spirit of the invention or surmount scope defined in appended claims.
Claims (9)
1. the electrochemical reactor of oligosaccharides, including the reactive tank (1) with cavity, its feature are prepared from marine organisms functional sugar
It is to be fixed with two pieces of dividing plates (2) in the reactive tank (1), cavity is separated into three chambers, three chambers by two pieces of dividing plates (2)
Room is reaction chamber (1a) and the flow cavity (1b) for being located at reaction chamber (1a) both sides respectively, and connection is provided with two pieces of dividing plates (2)
Pole plate component is provided with the opening (2a) of reaction chamber (1a) and flow cavity (1b), the reaction chamber (1a), pole plate component includes
Two pieces be arranged in parallel in pole plate (3) and positioned at two pieces while pole plate (3) between some Inner pole plates (4), on two pieces of side pole plates (3)
The mounting hole (3a) for installing electrode is provided with, the Inner pole plates (4) are parallel with side pole plate (3), the Inner pole plates (4) and side
Pole plate (3) is vertical with dividing plate (2), and the two ends of Inner pole plates (4) and the two ends of side pole plate (3) are respectively supported with two pieces of dividing plates (2)
Lean on, the Inner pole plates (4) and be provided between pole plate (3) by Inner pole plates (4) and while pole plate (3) be positioned at determining for reaction chamber (1a)
Bit architecture.
2. the electrochemical reactor of oligosaccharides is prepared in the functional sugar from marine organisms according to claim 1, it is characterised in that
The locating rod (5) that the location structure is made including insulating materials, the locating rod (5) is parallel with dividing plate (2), the locating rod
(5) two ends on the Inner pole plates (4) and side pole plate (3) respectively with the cell wall of reactive tank (1) against being provided with location hole, institute
The pore size for stating location hole is identical with the diameter of locating rod (5), the locating rod (5) through each Inner pole plate (4) and
Location hole on side pole plate (3), the locating rod (5) is provided with some positioning sleeves (51), and the positioning sleeve (51) is located at respectively
Between Inner pole plates (4) and side pole plate (3) and adjacent Inner pole plates (4).
3. the electrochemical reactor of oligosaccharides is prepared in the functional sugar from marine organisms according to claim 2, it is characterised in that
The two ends that the locating rod (5) passes side pole plate (3) are equipped with fixed cover (52), two pieces of side pole plates (3) respectively with fixed cover
(52) against the side of the fixed cover (52) offers fixing hole, and mounting hole corresponding with fixing hole is provided with locating rod (5)
(3a), fixed cover (52) is fixed in locating rod (5) by retaining pin (53) through fixing hole and mounting hole (3a).
4. the electrochemical reactor of oligosaccharides is prepared in the functional sugar from marine organisms according to claim 2, it is characterised in that
The locating rod (5) is provided between three location holes, three location holes successively with three on Inner pole plates (4) and side pole plate (3)
Line forms isosceles triangle, and three locating rods (5) are each passed through corresponding location hole on Inner pole plates (4) and side pole plate (3).
5. the electrochemical reaction of oligosaccharides is prepared in the functional sugar from marine organisms as claimed in any of claims 1 to 4
Device, it is characterised in that the mounting hole (3a) offers the middle part of the upper surface of side pole plate (3), on the hole wall of mounting hole (3a)
It is provided with internal thread.
6. the electrochemical reaction of oligosaccharides is prepared in the functional sugar from marine organisms as claimed in any of claims 1 to 4
Device, it is characterised in that two pieces of Inner pole plates (4) are with gap between the gap of side pole plate (3) and adjacent Inner pole plates (4) two
The opening (2a) being connected is respectively provided with block dividing plate (2).
7. oligosaccharides is prepared from marine organisms functional sugar using electrochemical reactor as claimed in any one of claims 1 to 6
Preparation method, it is characterised in that comprise the following steps：
A, charging：The appropriate mixed solution containing marine organisms functional sugar is delivered to the reactive tank of electrochemical reactor from storage tank
(1) in, the amount of the shitosan sodium chloride mixed solution of input judges according to the liquid level gauge (6) on electrochemical reactor；
B, reaction：Electrode on two side pole plates (3) on electrochemical reactor is connected with constant-current supply, to electrochemical reactor
The interior mixed solution containing marine organisms functional sugar carries out electrochemical degradation, and the reaction time is T；
C, discharging：The reaction product solution containing oligosaccharides and monose for completing will be reacted and be delivered to fluid reservoir from electrochemical reactor
The concentration of oligosaccharides in interior storage, and real-time detection reaction product solution；
D, separation and collection：Reaction product solution is delivered to chromatographic column from fluid reservoir, chromatographic column has some, chromatographic column it
Between be connected in series in successively, reaction product solution passes sequentially through some chromatographic columns, reaction product solution in every chromatographic column
Absorption oligosaccharides, wash-out monose, wash-out oligosaccharides are carried out successively and collect the oligosaccharides being eluted out；
By adsorbing oligosaccharides by weak acid polyacrylic acid type resin in step D；Monose is eluted by deionized water；By salt
Out, the concentration of hydrochloric acid is 0.07mol/l~0.13mol/l to the oligosaccharides eluted that acid will be adsorbed.
8. the preparation method of oligosaccharides is prepared in the functional sugar from marine organisms according to claim 7, it is characterised in that step
In C, to PID controller, PID controller carries out data with default data to the data transfer of the concentration of the oligosaccharides of real-time detection
Compare, and comparative result is transmitted to PLC, by PLC coordination electrode and constant-current supply Connection Time, from
And control the reaction time T of electrochemical reactor.
9. the preparation method of oligosaccharides is prepared in the functional sugar from marine organisms according to claim 7, it is characterised in that step
Chromatographic column is rinsed to neutrality by deionized water after oligosaccharides has been collected in D.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001247990A (en) * | 2000-03-03 | 2001-09-14 | Meiji Milk Prod Co Ltd | Method for manufacturing oligosaccharide using polymer-combined saccharide acceptor |
| CN1687139A (en) * | 2005-04-30 | 2005-10-26 | 浙江大学 | Method and equipment of integrated reaction and separation for preparing chitosan oligosaccharide |
| CN2858628Y (en) * | 2006-05-30 | 2007-01-17 | 冯庆中 | Pole-reversing operation electrochemical reactor |
| CN101654294A (en) * | 2009-09-23 | 2010-02-24 | 王万寿 | high-efficiency electrochemical reactor |
| CN102603038A (en) * | 2012-04-11 | 2012-07-25 | 苏州科技学院 | Electrochemical reactor and method for processing organic pollutant in water |
| CN203411372U (en) * | 2013-08-22 | 2014-01-29 | 吉林大学 | Turning flow type electrochemical reactor |
| CN204138782U (en) * | 2014-10-08 | 2015-02-04 | 林海波 | The electrochemical reactor of oligosaccharides is prepared from marine organisms functional sugar |
-
2014
- 2014-10-08 CN CN201410525615.6A patent/CN104233365B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001247990A (en) * | 2000-03-03 | 2001-09-14 | Meiji Milk Prod Co Ltd | Method for manufacturing oligosaccharide using polymer-combined saccharide acceptor |
| CN1687139A (en) * | 2005-04-30 | 2005-10-26 | 浙江大学 | Method and equipment of integrated reaction and separation for preparing chitosan oligosaccharide |
| CN2858628Y (en) * | 2006-05-30 | 2007-01-17 | 冯庆中 | Pole-reversing operation electrochemical reactor |
| CN101654294A (en) * | 2009-09-23 | 2010-02-24 | 王万寿 | high-efficiency electrochemical reactor |
| CN102603038A (en) * | 2012-04-11 | 2012-07-25 | 苏州科技学院 | Electrochemical reactor and method for processing organic pollutant in water |
| CN203411372U (en) * | 2013-08-22 | 2014-01-29 | 吉林大学 | Turning flow type electrochemical reactor |
| CN204138782U (en) * | 2014-10-08 | 2015-02-04 | 林海波 | The electrochemical reactor of oligosaccharides is prepared from marine organisms functional sugar |
Non-Patent Citations (1)
| Title |
|---|
| 电化学间接氧化降解壳聚糖;黄永春;《电化学间接氧化降解壳聚糖》;20101231;第21卷(第4期);第17-21页 * |
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