CN104224117B - Encoding a spectroscopic optical coherence tomography and confocal imaging method and system for collaborative - Google Patents

Encoding a spectroscopic optical coherence tomography and confocal imaging method and system for collaborative Download PDF

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CN104224117B
CN104224117B CN 201410459764 CN201410459764A CN104224117B CN 104224117 B CN104224117 B CN 104224117B CN 201410459764 CN201410459764 CN 201410459764 CN 201410459764 A CN201410459764 A CN 201410459764A CN 104224117 B CN104224117 B CN 104224117B
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CN104224117A (en )
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吴彤
施瑶瑶
刘友文
李艳
王吉明
赫崇君
顾晓蓉
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南京航空航天大学
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Abstract

本发明公开了一种光谱编码共焦与光学相干层析协同成像方法与系统,该系统基于光学相干层析(OCT)成像技术与光谱编码共焦显微(SECM)技术的结合,提出的将OCT系统的样品臂与SECM系统的样品臂共用同一光路,使得样品臂中从OCT系统与SECM系统出射的光束对样品同一位置同步进行扫描,并且从样品单次散射返回的光分别经各自的光路传输至探测臂。 The present invention discloses a coding spectral optical coherence tomography and confocal imaging methods and cooperative system based on optical coherence tomography (OCT) imaging and spectroscopy encoded confocal microscopy (the SECM) bonding technique, the OCT system proposed sample arm sample arm and SECM systems share the same optical path, such that the sample arm from the OCT system and SECM system beam exiting the sample in the same location simultaneously scanned, and the light scattered back from the sample a single respective via respective optical paths to probe arm. 提出的探测臂采用两个光纤准直镜以不同入射角照射光栅,使光栅衍射出两组无交叠出射的光谱信号,即为SECM和OCT的信号,本发明中的样品臂的光路更加简单,便于实施,光路也更加紧凑、稳定,系统的成像质量高,对生物组织结构成像更加精确。 Proposed probe arm using two optical fiber collimating lens irradiating the grating at different incident angles, so that the grating diffraction spectrum of the two non-overlapping signals emitted, i.e. SECM and OCT signal, the sample arm optical path in the present invention is much simpler , ease of implementation, the optical path is also more compact, stable, high quality imaging systems, and more accurate imaging of biological tissue structures.

Description

一种光谱编码共焦与光学相干层析协同成像方法与系统 Encoding a spectroscopic optical coherence tomography and confocal imaging method and system for collaborative

技术领域 FIELD

[0001] 本发明涉及光谱编码共焦显微技术与光学相干层析成像技术,尤其涉及一种光谱编码共焦与光学相干层析协同同步成像的技术。 [0001] The present invention relates to encoding the spectral confocal microscopy and optical coherence tomography, in particular, to a spectrum coding confocal optical coherence imaging synchronizing synergistic chromatography technique.

背景技术 Background technique

[0002] 光学相干层析成像(Optical Coherence Tomography,简称0CT)技术是一种新兴的生物医学光学成像技术,能实现对生物组织的结构与生理功能进行非接触、无损伤、高分辨率成像,在疾病的早期检测和在体活检领域有着广阔的应用前景。 [0002] Optical coherence tomography (Optical Coherence Tomography, referred 0CT) technology is an emerging biomedical optical imaging, can be realized on the structure and the physiological function of the biological tissue is a non-contact, non-invasive, high-resolution imaging, and it has broad application prospects in the field of body biopsy in early detection of the disease.

[0003] 光谱编码共焦显微(Spectrally Encoded Confocal Microscopy,简称SECM)技术是一种微侵入单模光纤的共焦成像方法,该技术采用宽带光源和衍射光栅实现同时探测样品多个横向位置点处的反射率。 [0003] spectrum encoded confocal microscopy (Spectrally Encoded Confocal Microscopy, referred to as the SECM) technique is a minimally invasive single mode optical fiber confocal imaging methods, the technique uses a broadband light source and the diffraction gratings at a plurality of samples simultaneously detecting the lateral position of the point Reflectivity. 通过检测样品臂的空间信息,光编码共焦显微系统可以免除机械扫描,提供分辨率趋于显微镜水平的活体生物组织图像,其紧密特性使其能够实现装入小直径导管或者内窥镜导管中。 By detecting the spatial information of the sample arm, the optical encoder can be dispensed with confocal microscopy mechanical scanning system, there is provided an image resolution living biological tissue tends microscope level, which make it possible to realize the tight property into a small diameter catheter or endoscopic catheter.

[0004] 相比而言,0CT技术的成像深度要大于SECM技术。 [0004] In contrast, the imaging depth is greater than the SECM 0CT art technology. 0CT技术能够提供高轴向分辨率的生物组织剖面层析图像,SECM技术能够提供高横向分辨率的生物组织浅表层横断面图像,两种技术在空间分辨率方面的优势互为补充,因此0CT技术与SECM技术的协同成像能够提供更高空间分辨率和更加全面的生物组织显微信息,在生物医学成像以及非生物材料检测等应用中都将具有重要的意义。 0CT technology capable of providing high axial resolution of the cross-sectional tomographic image of the biological tissue, the SECM techniques can provide cross-sectional images of biological tissues superficial high lateral resolution, the advantages of the two technologies in terms of spatial resolution are complementary, so 0CT SECM imaging technology and collaborative technology can provide higher spatial resolution and more comprehensive information microscopic biological tissue, it will have great significance in biomedical imaging and non-biological materials testing applications.

[0005] 目前所提出的0CT系统与SECM系统协同成像的方法中,大多是基于扫描振镜的摆动来实现两种系统间的开关切换,这种采用振镜作为机械式开关的系统结构较为复杂,并且没有做到真正意义上的同时扫描成像,如果被测样品是活体样品,就很有可能出现在切换过程中由于活体移动而造成的两个系统成像位置不对准的情况。 [0005] The method of the current system and the proposed 0CT synergistic imaged SECM systems, mostly achieved between the two switching systems based on scanning galvanometer of swing, such as a galvanometer system configuration using a mechanical switch is relatively complex , not done while scanning and forming a real sense, if the test sample is a biological sample, it is very when switching process due to movement of the living body imaging system caused by two positional misalignments may occur. 另外,在目前所提出的0CT系统与SECM系统协同成像的方法中,大多采用两个光源分别用于〇CT系统和SECM系统, 并且还采用了两个探测臂分别探测0CT系统的样品信号和SECM系统的样品信号,使得整合后的系统过于复杂,不够稳定,且搭建成本较高。 Further, in the method of the current system and the proposed 0CT synergistic imaged SECM system, two light sources are mostly used for 〇CT SECM systems and systems, and also using the two probe arms each detection system and the sample signal 0CT SECM the sample signal system, making the system after the integration is too complicated, is not stable enough, and build a high cost. 因此,如何在光路较为简单的情况下,设计出一种光谱编码共焦与光学相干层析协同成像方法与系统,实现真正意义上的同步扫描成像,同时减少光源及探测臂的数量,使协同成像系统结构更加紧凑稳定,就成为0CT系统与SECM系统协同成像系统研制的一大目标。 Thus, how the light path in the case of relatively simple design a coding spectral optical coherence tomography and confocal imaging methods and systems coordinated, synchronized scanning imaging a real sense, while reducing the number of light sources and the probe arm so that cooperative the imaging system more compact and stable, has become a major goal 0CT systems and collaborative systems SECM imaging system developed.

发明内容 SUMMARY

[0006]发明目的:为了克服现有技术中存在的不足,提出一种光谱编码共焦与光学相干层析协同成像方法与系统。 [0006] Object of the invention: In order to overcome the disadvantages present in the prior art, provides a spectrum coding optical coherence tomography and confocal imaging methods and systems synergistic. 该光谱编码共焦与光学相干层析协同成像方法与系统采用将光学相干层析成像(0CT)系统的样品臂与光谱编码共焦显微(SECM)系统的样品臂共用同一光路,使得样品臂中从0CT系统与SECM系统出射的光束对样品同一位置同步进行扫描,从样品单次散射返回的光分别经各自的光路传输至探测臂中的两个光纤准直镜,通过将这两个光纤准直镜以不同入射角照射光栅,使光栅衍射出两组无交叠出射的光谱信号,即为阳⑶和OCT的信号,经过聚焦镜头,分别聚焦于线阵电荷耦合器件(CCD)的左半部分像素和右半部分像素,即实现同时探测来自SECM和0CT系统的光谱信号。 The spectrum coding confocal optical coherence tomography synergistic image forming method and system using the sample arm the sample arm and the spectral code of the optical coherence tomography (0CT) system confocal microscopy (the SECM) systems share the same optical path, such that the sample arm from 0CT system SECM system sample beam emitted simultaneously scanned the same position, the light scattered back from the sample are transmitted through a single optical path to a respective probe arm of the two fiber collimator lens, the two straight-through fiber collimator mirror grating is illuminated at different incident angles, so that the grating diffraction spectrum of the signal emitted two non-overlapping, i.e. the male and ⑶ OCT signal, through the focusing lens, are focused to the left half of the linear charge-coupled device (CCD) of pixels and the right half of the pixel, i.e., to achieve simultaneous detection signal from the SECM and 0CT spectrum systems. 经传入计算机进行数据处理获取被测样品高轴向分辨率的0CT图像和高横向分辨率的SECM图像,实现0CT系统与SECM系统优势互补的协同成像。 Incoming data processing by a computer to obtain a test sample 0CT high axial resolution image and the high lateral resolution of SECM image, and achieve SECM system 0CT complementary synergistic imaging system.

[0007]为实现上述目的,本发明采用的技术方案为:一种光谱编码共焦与光学相干层析协同成像方法,将0CT系统的样品臂与SECM系统的样品臂共用同一光路,使得样品臂中从0CT系统与SECM系统出射的光束对样品同一位置同步进行扫描,从样品单次散射返回的光分别经各自的光路传输至探测臂中的两个光纤准直镜,通过将这两个光纤准直镜以不同入射角照射光栅,使光栅衍射出两组无交叠出射的光谱信号,该光谱信号即为SECM和0CT的信号,经过聚焦镜头,分别聚焦于线阵电荷耦合器件的左半部分像素和右半部分像素,即实现同时探测来自SECM和0CT系统的光谱信号。 [0007] To achieve the above object, the technical solution of the present invention is used are: encoding a spectroscopic optical coherence tomography and confocal imaging method synergy, the sample arm and the sample arm system 0CT SECM systems share the same light path, such that the sample arm 0CT system from SECM system sample beam emitted simultaneously scanned the same position, the light scattered back from the sample are transmitted through a single optical path to a respective probe arm of the two fiber collimator lens, an optical fiber by the two a collimator lens irradiating the grating at different incident angles, so that the grating diffraction spectrum of the two non-overlapping signals emitted, the signal spectrum is the SECM and 0CT signal, through the focusing lens, are focused on the left linear array of charge-coupled device and the right half pixel portion of the pixel, i.e., to achieve simultaneous detection signal from the SECM and 0CT spectrum systems.

[000S] —种基于权利要求1所述的光谱编码共焦与光学相干层析协同成像系统,包括光源(1)、参考臂(2)、样品臂(3)、探测臂(4)、传输光纤(5)、第一光隔离器(6)、第二光隔离器(7)、第一光纤耦合器(8)和第二光纤耦合器(9),所述第一光纤耦合器(8)的一端分别通过传输光纤(5)分别连接光源(1)和探测臂(4),而另一端通过传输光纤(5)分别连接样品臂(3)和第二光纤耦合器⑼的一端,且所述光源⑴与第一光纤耦合器⑻之间设置有第一光隔离器(6),第一光纤耦合器(8)和第二光纤耦合器(9)之间接有第二光隔离器(7);所述第二光纤耦合器(9)与第一光纤耦合器(8)连接的一端还与探测臂(4)连接,而所述第二光纤親合器⑼的另一端分别连接参考臂⑵和样品臂(3)。 [000S] - species based on the spectral encoder according to claim 1 optical coherence tomography and confocal imaging synergistic system comprising a light source (1), the reference arm (2), the sample arm (3), the probe arm (4), the transmission an optical fiber (5), a first optical isolator (6), a second optical isolator (7), a first fiber coupler (8) and a second fiber coupler (9), said first fiber coupler (8 ) are connected to one end of an optical fiber via a transmission (5) are the light source (1) and the probe arm (4), and the other end via a transmission optical fiber (5) are connected to the sample arm (3) and the end of the second fiber coupler ⑼, and the light source is provided with a first optical isolator (6), a first fiber coupler (8) and a second fiber coupler (9) has an indirect optical isolator between the second and the first optical coupler ⑴ ⑻ ( 7); one end of said second optical coupler (9) is connected to a first fiber coupler (8) is also connected to the probe arm (4), and the other end of said second fiber coupler ⑼ affinity are connected to the reference ⑵ arm and the sample arm (3).

[0009]所述样品臂⑶包括按光速流向依次设置的第一光纤准直镜(10)、第一光栅(U)、 第一凸透镜(12)、第二凸透镜(13)以及显微物镜(16),所述第一光纤耦合器⑻和第二光纤耦合器(9)连接的一端与第一光纤准直镜(1〇)相连,且所述第一光栅(11)的光斑中心、第一凸透镜(12)中心、第二凸透镜(13)中心以及显微物镜中心在同一条光轴上;以第一凸透镜(12)与第二凸透镜(13)光轴连线方向为Z轴,以第二光纤准直镜(14)和振镜(15)的光轴连线方向为Y轴,以第一凸透镜的中心为原点,根据右手定则,建立坐标系;所述第一光栅(11) 表面垂直于YZ平面,且所述第一光栅(11)与XZ的夹角为a;还包括按光束传播顺序依次设置的第二光纤准直镜(14)和振镜(15),所述第二光纤耦合器(9)和参考臂(2)连接的一端与第二光纤准直镜(14)连接,所述振镜(15)设置于第一光栅(11)与第一 [0009] The sample arm comprising a first optical fiber ⑶ collimator lens (10) by the flow of light are sequentially arranged, the first grating (the U-), first convex lens (12), a second lens (13) and microscope objective ( 16), one end of the first optical fiber and a second fiber coupler ⑻ coupler (9) connected to the first optical fiber collimator lens (1〇) is connected, and the first grating (11) of the spot center, the first a convex lens (12) center, a second lens (13) and the center of a microscope objective centered on the same optical axis; in (12) and a second convex lens (13) to connect the optical axis direction of the Z axis of the first lens to connecting the optical axis direction of the second optical fiber collimator lens (14) and a galvanometer (15) is Y-axis, a first lens center as the origin, according to the right hand rule coordinate system is established; the first grating (11 ) surface perpendicular to the YZ plane, and the first grating (11) for the XZ angle a; a second optical fiber further comprises a collimator lens (14) and a galvanometer (15) by propagating light beams are sequentially arranged sequentially, the One end of the second optical fiber collimator lens (14) of said second fiber optic coupler (9) and a reference arm (2) connected to the connector, the galvanometer (15) disposed on the first grating (11) and the first 凸透镜(12)之间,所述振镜(I5)表面垂直于YZ平面,且振镜(15)与第二光纤准直镜(14)的高度在同一水平面,另外所述第二光纤准直镜(14)和振镜(15)在第一光栅(11)上的光斑下方沿正X轴方向的偏移量>0;所述第一光纤准直镜(10)将来自第一光纤耦合器(8)的光束投射到第一光栅(11)表面上形成光斑,从第一光栅衍射出的光谱投射在第一凸透镜(I2)的上半部分;第二光纤准直镜(14)将来自第二光纤耦合器(9)的光束投射在振镜(15)上,该光束经过振镜(15)反射在第一凸透镜(12)的下半部分,通过调整第一光栅(11)放置角度a和振镜(lf5)的扫描范围使得从振镜(15)反射的光束的扫描角范围与第一光栅(11)出射光的角色散范围相等;投射在第一凸透镜(12)的上下两部分光束经过第一凸透镜(12)后,在第一凸透镜(12)的像方焦平面聚焦成同一条水平方向的亮线斑,这条亮线 Between the convex lens (12), said galvanometer (I5) to the YZ plane perpendicular to the surface, and a galvanometer (15) and the height of the second optical fiber collimator lens (14) in the same horizontal plane, the further second fiber collimators a mirror (14) and a galvanometer (15) offset in the positive X-axis direction below the spot (11) in the first grating> 0; the first optical fiber collimator lens (10) from the first fiber optic coupler a beam (8) is projected to the first grating (11) is formed on the surface of the light spot, a first diffraction grating from the spectral transmittance of the first lens (I2) in the upper part; a second optical fiber collimator lens (14) projecting a light beam from the second fiber coupler (9) in a galvanometer (15), through the galvanometer beam (15) reflected in the lower half of the first convex lens (12) is divided, by adjusting the placement of the first grating (11) angle and a galvanometer (LF5) from the sweep range such that the galvanometer (15) and the scan angle range of the first grating (11) the reflected beam is equal to the angular dispersion range of the emitted light; a first vertically projecting convex lens (12) after the first convex part-beams (12) passes, the first convex lens (12) is focused into the same focal plane as a horizontal bright line spots, this bright line 斑在中点处与第一凸透镜(12)的光轴垂直正交,并继续入射到第二凸透镜(13)上;从第二凸透镜(13)出射的光束得到会聚,并全部入射到显微物镜(16)中,经过显微物镜(16)聚焦的光束投射在样品上;从样品单次散射返回的光经原光路返回并分别经过第一光纤准直镜(10)、第二光纤准直镜(14)返回各自的光路,并进入探测臂。 A vertical axis perpendicular to the midpoint of the spot from the first convex lens (12) and continues on the second lens is incident (13); obtained from the second condensing lens (13) emitted beam incident on the microstructure and all an objective lens (16), through the microscope objective (16) projecting a light beam focused on the sample; and each return (10), a second optical fiber collimator collimator lens through the first primary light is scattered back from the optical path of a single sample collimator lens (14) return to their optical path, and enters the detection arm.

[0010] 所述探测臂(4)包括第三光纤准直镜(17)、第四光纤准直镜(18)、第二光栅(19)、 聚焦镜头(20)和线阵电荷耦合器件(21);所述第一光纤耦合器⑻和光源(1)连接的一端与第四光纤准直镜(18)连接,而所述第二光纤耦合器⑼和第一光纤耦合器⑻连接的一端与第三光纤准直镜(17)连接;所述第三光纤准直镜(17)和第四光纤准直镜(18)在第二光栅(19) 的同一侧,且所述第三光纤准直镜(17)和第四光纤准直镜(18)分别与第二光栅(19)成不同的夹角放置;而所述第二光栅(19)另一侧放置线阵电荷耦合器件(21),所述聚焦镜头(20) 放置在第二光栅(19)与线阵电荷耦合器件(21)之间;所述第三光纤准直镜(17)收集来自于第一光纤耦合器(8)的光束,而所述第四光纤准直镜(18)收集来自于第二光纤耦合器(9)的光束,且所述第三光纤准直镜(17)、第四光纤准直镜(18)分别以不同的入 [0010] The detection arm (4) comprises a third optical fiber collimator lens (17), a fourth optical fiber collimator lens (18), a second grating (19), focusing lens (20) and the line charge coupled device ( 21); one end of said first fiber optic coupler ⑻ and the light source (1) connected to the fourth optical fiber collimator lens (18), and said second optical coupler and a first optical coupler ⑼ ⑻ connected to one end connected to the third optical fiber collimator lens (17); said third optical fiber collimator lens (17) and a fourth optical fiber collimator lens (18) on the same side of the second grating (19), and said third optical fiber collimator lens (17) and a fourth optical fiber collimator lens (18) are placed into a different angle of the second grating (19); and said second grating (19) disposed on the other side a linear array charge coupled device ( 21), said focusing lens (20) disposed between the second grating (19) with a charge coupled device linear array (21); said third optical fiber collimator lens (17) collected from the first fiber coupler ( 8) beam, and the fourth fiber collimator lens (18) collects the light beam from the second fiber coupler (9), and said third optical fiber collimator lens (17), a fourth optical fiber collimating lens (18) respectively into different 射角度将两个光束投射到第二光栅(19)上,使第二光栅(19)衍射出两组无交叠出射的光谱信号,即为SECM和OCT的信号,经过聚焦镜头(20),分别聚焦于线阵CCD (21)光敏面的左半部分像素和右半部分像素。 The two beams exit angle is projected onto the second grating (19), the second grating (19) diffract the two non-overlapping spectral emission signal, namely a signal OCT and SECM, through the focusing lens (20), They are focused on (21) linear CCD photosensitive surface of the left half pixels and the right half pixels.

[0011] 优选的:所述第一凸透镜(12)与第二凸透镜(13)之间的距离为两透镜的焦距之和,第二凸透镜(13)与显微物镜(16)的距离等于第二凸透镜(13)的焦距。 [0011] Preferably: the distance between the first lens (12) and a second convex lens (13) is the focal length of the lens and the two, second convex lens (13) and microscope objective (16) a distance equal to the two convex lens (13) focal length. 所述两组光谱信号无交叠地聚焦在CCD光敏面上。 The two non-overlapping signal spectra focused on the photosensitive surface of the CCD.

[0012] 本发明提供的一种光谱编码共焦与光学相干层析协同成像方法与系统,相比现有技术,具有以下有益效果: [0012] The present invention provides a spectroscopic encoding a confocal optical coherence tomography imaging methods and systems synergistic, compared with the prior art, it has the following advantages:

[0013] 1、通过采用将光学相干层析成像系统的样品臂与光谱编码共焦显微系统的样品臂共用同一光路,使得样品臂中从0CT系统与SECM系统出射的光束对样品同一位置同步进行扫描,有效克服了将两系统结合时使用机械切换所带来的问题,真正做到了同步扫描样品。 [0013] 1, by using the sample arm and the spectral code of the optical coherence tomography system confocal sample arm microscopy systems sharing the same optical path, such that the sample arm to the sample in the same position synchronization scan from 0CT system SECM system beam exiting effectively overcome the problem of mechanical bonding two switched system brought, truly synchronous scanning samples. 另外,相比之前的方法和系统,本发明中的样品臂的光路更加简单,便于实施,光路也更加紧凑、稳定。 Further, compared to the previous methods and systems, the sample arm optical path in the present invention is more simple, easy to implement, the optical path is also more compact and stable.

[0014] 2、通过采用光谱编码共焦与光学相千层析协同成像方法与系统,使得该系统的轴向分辨率相对于单独的SECM系统有了很大提高,并且使该系统的横向分辨率相对于单独的OCT系统得到很大提高,极大地优化了系统的成像质量。 [0014] 2, by using the optical spectral phase encoding confocal imaging one thousand synergistic chromatographic methods and systems, such that the axial resolution of the system with respect to individual SECM system has been greatly improved, and the lateral resolution of the system rate with respect to OCT systems alone greatly improved greatly optimize the image quality of the system.

[0015] 3、通过采用将光学相千层析成像系统的样品臂与光谱编码共焦显微系统的样品臂共用同一光路,只需要采用一个光源;另外,通过采用将光学相干层析成像系统与光谱编码共焦显微系统在探测臂中实现同时探测的光路,使得〇CT系统与SECM系统实现同时探测成像,这种探测方法省去了同时使用两个探测臂来探测信号的麻烦,只需要用一个探测臂同时探测0CT系统和SECM系统的光谱信号,使得系统结构更加紧凑,光路更加稳定,并且节约了搭建成本。 [0015] 3, share the same optical path by using the sample arm and the spectral code of the optical phase one thousand tomography system confocal microscopy system, a sample arm, only need a light source; Further, by using the optical coherence tomography system and Spectra encoded confocal microscopy system to achieve simultaneous detection of light in the detection arm, such that the SECM and 〇CT system implemented simultaneously detecting and imaging system, this detection method eliminates the need to use both the probe arm to the trouble detection signal, only need a probe while the arm detecting spectroscopic signals 0CT SECM systems and systems, making the system more compact, more stable optical path, and save the cost of building.

[0016] 4、通过采用光谱编码共焦与光学相干层析协同成像方法与系统,使得SECM系统和0CT系统的成像结果相互配准、像素对齐,从而使该系统对生物组织结构成像更加精确。 [0016] 4, by using the spectral code of the confocal optical coherence tomography imaging methods and systems coordinated so that the imaging system and the results of SECM systems 0CT mutual registration, pixel alignment, so that the system is more accurate imaging of biological tissue structures.

附图说明 BRIEF DESCRIPTION

[0017] 图1是本发明的结构示意图; [0017] FIG. 1 is a structural diagram of the present invention;

[0018] 图2是样品臂的示意图; [0018] FIG. 2 is a schematic view of the sample arm;

[0019]图3是探测臂的示意图; [0019] FIG. 3 is a schematic view of the probe arm;

[0020]图中:1、光源,2、参考臂,3、样品臂,4、探测臂,5、传输光纤,6、第一光隔离器,7、第二光隔离器,8、第一光纤耦合器,g、第二光纤耦合器,10、第一光纤准直镜,n、第一光栅, 12、第一凸透镜,13、第二凸透镜,14、第二光纤准直镜,15、振镜,I6、显微物镜,17、第三光纤准直镜,18、第四光纤准直镜,19、第二光栅,20、聚焦镜头,21、线阵电荷耦合器件(CCD)。 [0020] FIG: 1, a light source 2, the reference arm 3, the sample arm 4, the probe arm 5, a transmission optical fiber 6, a first optical isolator 7, a second optical isolator 8, a first fiber coupler, g, a second optical coupler 10, a first optical fiber collimator lens, n, a first grating 12, a first lens 13, second lens 14, a second optical fiber collimating lens, 15, galvanometer, I6, microscope objective 17, the third optical fiber collimator 18, a collimator lens of the fourth optical fiber 19, a second grating 20, focusing lens 21, linear charge-coupled device (CCD).

具体实施方式 detailed description

[0021]下面结合附图和实施例对本发明作进一步的说明,本发明的目的和效果将变得更加明显。 [0021] The following embodiments in conjunction with the drawings and embodiments of the present invention will be further explained, the purpose and effect of the present invention will become more apparent.

[0022] —种光谱编码共焦与光学相干层析协同成像方法,如图1-3所示,将OCT系统的样品臂与SECM系统的样品臂共用同一光路,使得样品臂中从OCT系统与SECM系统出射的光束对样品同一位置同步进行扫描,从样品单次散射返回的光分别经各自的光路传输至探测臂中的两个光纤准直镜,通过将这两个光纤准直镜以不同入射角照射光栅,使光栅衍射出两组无交叠出射的光谱信号,该光谱信号即为SECM和OCT的信号,经过聚焦镜头,分别聚焦于线阵电荷耦合器件的左半部分像素和右半部分像素,即实现同时探测来自SECM和OCT系统的光谱信号。 [0022] - the kind of spectrum coding confocal optical coherence tomography imaging method synergistic, shown in Figure 1-3, the sample arm and the sample arm of the OCT system SECM systems share the same optical path, such that the sample arm from the OCT system SECM system sample beam emitted synchronously scanning the same position, the light scattered back from the sample, respectively, via respective single optical transmission path to the two optical fibers in the probe arm collimator lens, the two optical fibers through a collimator lens at different irradiating the grating angle of incidence, the diffraction grating so that two non-overlapping spectral signal is emitted, the signal spectrum is the SECM and OCT signal, through the focusing lens, are focused to the left half of the linear charge-coupled device pixels and right portion of the pixel, i.e., to achieve simultaneous detection signal from the SECM and spectral OCT system.

[0023] —种基于权利要求1所述的光谱编码共焦与光学相干层析协同成像系统,如图! [0023] - based on the spectral code of the kind claimed in claim 1, optical coherence tomography and confocal imaging system cooperative FIG! 所示,包括光源1、参考臂2、样品臂3、探测臂4、传输光纤5、第一光隔离器6、第二光隔离器7、第一光纤耦合器8和第二光纤耦合器9,所述第一光纤耦合器8的一端分别通过传输光纤5分别连接光源1和探测臂4,而另一端通过传输光纤5分别连接样品臂3和第二光纤耦合器9的一端,且所述光源1与第一光纤親合器8之间设置有第一光隔离器6,第一光纤親合器8和第二光纤耦合器9之间接有第二光隔离器7;所述第二光纤耦合器9与第一光纤耦合器8连接的一端还与探测臂4连接,而所述第二光纤耦合器9的另一端分别连接参考臂2和样品臂3。 Shown, including 1, 2 reference arm, the sample arm 3, probe arm 4, the transmission optical fiber 5, a first optical isolator 6, a second optical isolator 7, a first optical coupler 8 and the second light source fiber coupler 9 end of the first optical fiber coupler 8 are connected via a transmission fiber 1 and the light source 5 each probe arm 4, and the other end connected to one end of the sample arm 3 and the second fiber coupler 9 through the transmission fiber, respectively, 5 and the 8 disposed between the light source 1 and the first optical fiber has a first affinity optical isolator 6, a first optical fiber 8 and the second affinity optical coupler 9 of the second optical isolator indirectly 7; the second optical fiber 9 further coupler 4 is connected to the first end of the probe arm 8 is connected to the fiber coupler, and the other end of said second fiber coupler 9 are connected to the reference arm and the sample arm 3 2.

[0024] 光源1发出的宽带光经过传输光纤5和第一光隔离器6传输至第一光纤耦合器8,并被分为两束光分别传输至样品臂3中的第一光纤准直镜10和第二光隔离器7。 [0024] 1 light source emits broadband light transmitting fiber through a first optical isolator 5 and 6 is transmitted to the first optical coupler 8, and is divided into a first optical fiber collimator in two beams are respectively transmitted to the sample arm collimator lens 3 and a second optical isolator 10 7. 从第一光纤准直镜10入射的光束经样品臂3反射回来,再次经过第一光纤耦合器8和传输光纤5传输至探测臂4中的第四准直镜18并进行探测。 Reflected from the first optical fiber collimator 10 through the light beam incident sample arm 3 back in again through the first optical coupler 5 and optical fiber 8 is transmitted to the fourth probe arm 4 and the collimator lens 18 to be detected. 从第二光隔离器7出射的光束经过第二光纤耦合器9 并被分为两部分,一部分光束进入参考臂2并反射回来,另一部分光束进入样品臂3的第二光纤准直镜14并经过样品臂3的反射回来,反射回来的两束光经过第二光纤耦合器9耦合并发生干涉,干涉光经过传输光纤5入射探测臂4中的第三光纤准直镜17并进入探测臂中探测。 From the second optical isolator 7 emitted light passes through the second optical coupler 9 and is divided into two parts, into the portion of the beam 2 and the reference arm is reflected back, another portion of the sample arm light beam enters the second optical fiber collimator lens 3 and 14 9 after coupling the reflected sample arm back 3, two beams reflected through the second optical coupler and interfere with the interference light transmission through the optical fiber 5 is incident on the third optical fiber probe arm 4 and the collimator lens 17 enters the detection arm detection.

[0025] 参考臂2用于提供参考光信号。 [0025] Referring arm 2 for providing a reference optical signal. 典型的参考臂由光纤准直镜、凸透镜和固定在平移台上的反射镜组成。 A typical optical fiber reference arm collimating lens, a convex lens and a fixed composition at a translation stage mirror.

[0026] 光隔离器是一种能够吸收或者偏移后向传输的磁光设备。 After [0026] An optical isolator is capable of absorbing or shift the transmission to a magneto-optical device. 光纤隔离器一般用来保护光源免受反向反射的影响,防止强度噪声引起的光学损坏。 Fiber isolator generally used to protect against reverse reflection light, to prevent damage to the optical intensity noise. 典型的光隔离器参数可参考美国的Thorlabs公司生产的光隔离器。 A typical parameters refer to the optical isolator US Thorlabs' optical isolator.

[0027] 光纤耦合器是一种用于实现光信号分路或合路的元件。 [0027] The optical fiber coupler is a device for realizing an optical combiner or the signal splitter. 典型的光隔离器参数可参考美国的Thorlabs公司生产的光纤耦合器。 A typical parameters refer to the optical isolator US Thorlabs' optical fiber coupler.

[0028] 如图2所示,所述样品臂3包括按光速流向依次设置的第一光纤准直镜10、第一光栅11、第一凸透镜12、第二凸透镜13以及显微物镜ie,所述第一光纤耦合器8和第二光纤耦合器9连接的一端与第一光纤准直镜10相连,且所述第一光栅11的光斑中心、第一凸透镜12 中心、第二凸透镜13中心以及显微物镜中心在同一条光轴上;以第一凸透镜12与第二凸透镜13光轴连线方向为Z轴,以第二光纤准直镜14和振镜15的光轴连线方向为Y轴,即如图2所示,垂直纸面方向且垂直于第一凸透镜12与第二凸透镜13中心连线的方向为Y轴,以第一凸透镜的中心为原点,根据右手定则,建立坐标系;所述第一光栅11表面垂直于YZ平面,且所述第一光栅11与XZ的夹角为a;还包括按光束传播顺序依次设置的第二光纤准直镜14和振镜15,所述第二光纤耦合器9和参考臂2连接的一端与第二光纤准直镜14 [0028] As shown in FIG. 2, the sample arm comprising a first optical fiber 3 by a collimator lens 10 arranged sequentially flow speed of light, a first grating 11, a first convex lens 12, and a second microscope objective lens 13 IE, the said first optical coupler 8 and the second fiber coupler 9 has one end connected to the first optical fiber connected to collimating lens 10, and the first grating 11 of the spot center, the center of the first lens 12, second lens 13 and the center microscope objective centered on the same optical axis; a first convex lens 12 and the second optical axis 13 to connect the Z-axis direction, a second collimator lens 14 and the optical resonator mirror 15 is an optical axis direction connecting Y axis, i.e., FIG. 2, a direction perpendicular to the paper and perpendicular to the first direction of the second convex lens 12 and the lens 13 as a Y-axis line of centers, with the center of the first convex lens as an origin, according to the right-hand rule, to establish the coordinates line; the first grating surface 11 perpendicular to the YZ plane, and the angle of the first grating 11 and XZ is a; a second optical fiber further comprises a light beam by propagating the sequence are sequentially arranged collimator lens 14 and the galvanometer 15, the second end of the fiber coupler 9 and a reference arm optical fiber 2 connected to the second collimator lens 14 接,所述振镜15 设置于第一光栅11与第一凸透镜12之间,所述振镜15表面垂直于YZ平面,且振镜15与第二光纤准直镜14的高度在同一水平面,另外所述第二光纤准直镜14和振镜15在第一光栅11上的光斑下方沿正X轴方向的偏移量>0; Then, the galvanometer 15 is provided between the first grating 11 and the first convex lens 12, the galvanometer 15 to a surface perpendicular to the YZ plane, and the height of the galvanometer 15 and the second collimator lens 14 of the optical fiber at the same level, Further the second optical fiber collimator lens 14 and the galvanometer 15 under the spot on the first grating 11 in the positive X-axis direction shift amount of> 0;

[0029] 所述第一光纤准直镜10将来自第一光纤耦合器8的光束投射到第一光栅11表面上形成光斑,从第一光栅衍射出的光谱投射在第一凸透镜12的上半部分;第二光纤准直镜14 将来自第二光纤耦合器9的光束投射在振镜15上,该光束经过振镜15反射在第一凸透镜12 的下半部分,通过调整第一光栅11放置角度a和振镜15的扫描范围使得从振镜15反射的光束的扫描角范围与第一光栅11出射光的角色散范围相等。 [0029] The collimator lens 10 of the first optical fiber from the first fiber coupler is a light beam 8 onto the surface grating 11 is formed a first light spot, the upper half diffraction grating from the first spectral transmittance of the first convex lens 12 section; a second optical fiber collimator lens 14 from the second beam onto the fiber coupler 9 in the vibration mirror 15, the beam passes through the resonator mirror 15 in the lower portion of the first convex lens 12, by adjusting the placement of the first grating 11 and a galvanometer scanning angle range of 15 so that the scanning angle range of the first grating 15 from the light beam reflected by the oscillating mirror 11 is equal to the angular dispersion of light emitted range. 投射在第一凸透镜12的上下两部分光束经过第一凸透镜12后,在第一凸透镜12的像方焦平面聚焦成同一条水平方向的亮线斑,这条亮线斑在中点处与第一凸透镜12的光轴垂直正交,并继续入射到第二凸透镜13上; 从第二凸透镜13出射的光束得到会聚,并全部入射到显微物镜16中,经过显微物镜16聚焦的光束投射在样品上;从样品单次散射返回的光经原光路返回并分别经过第一光纤准直镜10、第二光纤准直镜14返回各自的光路,并进入探测臂。 Projected two parts of the beam of the first lens after the first lens 12 12, a first convex lens on the image side focal plane 12 is focused to the same spot in the horizontal direction of the bright line, this bright line at the midpoint of the first spot a convex lens perpendicular to the optical axis 12 and continues onto a second lens 13 is incident; obtained from condensing the second light beam 13 emitted convex lens, and all incident to the microscope objective 16, the beam passing through the microscope objective 16 focuses the projected on a sample; return light from the sample through the original optical path of the single scattering and are returned via the first optical fiber collimator lens 10, a second optical fiber collimator lens 14 return to their respective optical paths, and into the probe arm. 所述第一凸透镜12与第二凸透镜13 之间的距离为两透镜的焦距之和,第二凸透镜13与显微物镜16的距离等于第二凸透镜13的焦距。 The distance between the first 12 and the second lens 13 is a convex lens and the focal length of the two lenses, the second lens 13 and the distance 16 is equal to the focal length of the microscope objective lens 13 of a second. 所述两组光谱信号无交叠地聚焦在CCD光敏面上。 The two non-overlapping signal spectra focused on the photosensitive surface of the CCD.

[0030] 具体的:第一光纤准直镜10收集来自光谱编码共焦显微SECM系统的光束并将其投射到第一光栅11上,从第一光栅衍射出的光谱投射在第一凸透镜12的上半部分,第二光纤准直镜14收集来自光学相干层析成像0CT系统的光束并通过振镜15的反射投射在第一凸透镜12的下半部分,通过调整第一光栅11放置角度和振镜15的扫描范围使得从振镜15反射的光束的扫描角范围与第一光栅11出射光的角色散范围相等。 [0030] Specifically: a first and projected onto grating 11, the first optical fiber collimator lens 10 collects the light beam from the coded spectral confocal microscopy SECM system first diffraction grating from the spectral transmittance of the first convex lens 12 half of the second optical fiber from the collimator lens 14 collects light beams 0CT optical coherence tomography system and in the lower half of the first lens 12 is reflected by the galvano mirror 15 is projected by adjusting the first angle and the grating 11 is placed galvanometer 15 so that the scanning range of the scanning angle range of the first grating 15 from the light beam reflected by the oscillating mirror 11 is equal to the angular dispersion of light emitted range. 投射在第一凸透镜12的上下两部分光束经过第一凸透镜12后,在第一凸透镜12的像方焦平面聚焦成一条水平方向的亮线,这条亮线在中点处与第一凸透镜12的光轴垂直正交,自此两束光发生耦合,并继续入射到第二凸透镜13中。 A first projection lens 12 of the upper and lower portion of the beam after the first lens 12, the first convex lens on the image side focal plane 12 is focused into a bright line in the horizontal direction, this bright line at the midpoint of the first convex lens 12 orthogonal to the optical axis, since two beams of light coupling occurs, and continues to the second lens 13 is incident. 从第二凸透镜13出射的光束相比入射第一凸透镜12时得到收敛,并全部入射到显微物镜I6中,经过显微物镜ie聚焦的光束投射在样品上。 12:00 to give the incident first convex lens 13 compared to the light emitted from the second convergent lens, and incident on the microscope objective all I6, after ie microscope objective focusing beam onto the sample. 从样品单次散射返回的光经原光路返回并分别经过第一光纤准直镜10、第二光纤准直镜14返回各自的光路,并进入探测臂。 Return light from the sample through the original optical path of the single scattering and are returned via the first optical fiber collimator lens 10, a second optical fiber collimator lens 14 return to their respective optical paths, and into the probe arm.

[0031]光栅能够使光束发生色散,使不同波长的光依次打在样品上,从而实现光谱编码。 [0031] The dispersion grating light beam can occur, so that light of different wavelengths are sequentially hit on the sample in order to achieve spectral coding. 光栅可分为透射式衍射光栅和反射式衍射光栅,典型的光栅参数可参考美国的Wasatch Photonics公司生产的光栅。 Grating can be divided into transmissive diffraction grating and the reflection type diffraction grating, the grating parameters typically refer to US Wasatch Photonics Company grating.

[0032]光纤准直镜由尾纤与自聚焦透镜精确定位而成,它可以将光纤发出的发散光转变成准直平行的空间光。 [0032] by a fiber pigtail collimator lens GRIN precise positioning is made, it can be converted to divergent light emitted from the optical fiber into a collimated parallel light space.

[0033] 振镜I5能够根据电脑控制器发出的驱动信号,使其光学扫描头实现绕轴转动,从而使得从该光学扫描头反射的光束在一个平面内扫描。 [0033] I5 galvanometer drive signal can be issued by the controller computer so as to realize the optical scanning head is rotated about an axis such that the beam reflected from the optical scanning head in a scanning plane. 常用于OCT系统的样品臂中来实现光束对样品不同位置处的扫描。 Commonly used in the sample arm of an OCT system for scanning beam be achieved at different locations of the sample.

[0034]显微物镜16能够将样品臂的光束高度聚焦,使打到样品上的光点更小,从而使系统的横向分辨率得到提高。 [0034] The microscope objective 16 can be highly focused beam sample arm, so that hit the light spot on the sample is smaller, so that the lateral resolution of the system is improved.

[0035]如图3所示,所述探测臂4包括第三光纤准直镜17、第四光纤准直镜18、第二光栅19、聚焦镜头20和线阵电荷耦合器件21;所述第一光纤耦合器8和光源1连接的一端与第四光纤准直镜18连接,而所述第二光纤耦合器9和第一光纤耦合器8连接的一端与第三光纤准直镜17连接;所述第三光纤准直镜17和第四光纤准直镜18在第二光栅19的同一侧,且所述第三光纤准直镜17和第四光纤准直镜18分别与第二光栅19成不同的夹角放置;而所述第二光栅19另一侧放置线阵电荷耦合器件21,所述聚焦镜头20放置在第二光栅19与线阵电荷耦合器件21之间。 [0035] As shown in FIG. 3, the detection arm 4 comprises a third optical fiber collimator lens 17, a fourth fiber collimator mirror 18, a second grating 19, focusing lens 20 and the line charge coupled device 21; the first one end of an optical fiber coupler 1 and the light source 8 connected to the fourth optical fiber collimator 18 is connected, and one end of said second optical coupler 9 and the first optical coupler 8 is connected to the third optical fiber collimator 17 is connected; the third optical fiber collimator lens 17 and the fourth optical fiber collimator lens 18 on the same side of the second grating 19, and the third optical fiber collimator lens 17 and the fourth fiber collimator mirror 18 and the second grating 19, respectively, placing a different angle; and the second grating 19 disposed on the other side a linear array charge-coupled device 21, a focusing lens 20 disposed between grating 19 and the second linear array charge coupled device 21.

[0036] 所述第三光纤准直镜17收集来自于第一光纤耦合器8的光束,而所述第四光纤准直镜18收集来自于第二光纤耦合器9的光束,且所述第三光纤准直镜17、第四光纤准直镜18 分别以不同的入射角度将两个光束投射到第二光栅19上,使第二光栅19衍射出两组无交叠出射的光谱信号,即为SECM和0CT的信号,经过聚焦镜头20,分别聚焦于线阵CCD21光敏面的左半部分像素和右半部分像素。 [0036] The third optical fiber collimator 17 collected from the first fiber coupler of the beam 8, and the fourth fiber collimator mirror 18 to collect the light beam 9 from the second fiber coupler, said first and three fiber collimator 17, a collimator lens 18 of the fourth optical fiber at different angles of incidence, respectively, the two beams 19 projected onto second grating, the second diffraction grating 19 the two non-overlapping spectral signal emitted, i.e., SECM 0CT signal and, after the focus lens 20, are focused on the left half of the pixel of the linear array and the photosensitive surface CCD21 right half pixels.

[0037] 具体的:第三光纤准直镜17、第四光纤准直镜18分别收集OCT系统和SECM系统的光束,并以不同的入射角度将来自两个系统的光束投射到第二光栅19上,通过计算和调整这两个入射角的大小,使第二光栅19衍射出两组无交叠出射的光谱信号,即为SECM和0CT的信号,且其中一组光谱信号的最大波长对应的出射角与另一组光谱信号的最小波长对应的出射角相等,这样这两组光谱信号分别在左右两边的空间中传播,且两组光谱信号紧紧相邻, 随后投射到聚焦镜头20中。 [0037] Specifically: a third optical fiber collimator lens 17, a fourth optical fiber 18 are collected by a collimator lens system and the OCT beam SECM systems and different angles of incidence of the light beams from the two systems is projected onto second grating 19 , through calculation and adjustment of the angle of incidence of the two sizes, the second diffraction grating 19 the two non-overlapping spectral emission signals, namely the signal and 0CT SECM, and wherein a set of spectral signal maximum wavelength corresponding to It is equal to the minimum wavelength exit angle of the other set of spectral signal corresponding to an angle, so that the two sets of spectral signals are left and right sides of the propagation space, and two closely adjacent spectral signal, is then projected onto the focusing lens 20. 经过聚焦镜头20的聚焦,使得这两组光谱信号在线阵CCD21的光敏面上聚焦成一条亮线,这条亮线的左、右半边分别为SECM系统和0CT系统的光谱信号,SP 线阵CCD21感光面的左半部分和右半部分分别探测SECM系统和0CT系统的光谱信号。 After the focus of the focus lens 20, so that the two sets of line spectral signal array on the photosensitive surface of the CCD21 focused into a bright line, bright lines of this left, the right half of the signal spectrum SECM systems are systems and 0CT, SP linear CCD21 left and right halves, respectively, the photosensitive surface of the detection system and the spectral signal 0CT SECM systems.

[0038]线阵CCD21能够实时的将光强信号转化为电压信号,电压信号经数据传输线缆传输到计算机进行采集。 [0038] Linear CCD21 real time the intensity of the signal into a voltage signal, the voltage signal through the data transmission cable to the computer acquisition. 典型的线阵CCD参数可参考法国Atmel公司生产的线阵CCD(Aviiva SM2)。 A typical linear CCD French specifications can be produced by Atmel linear array CCD (Aviiva SM2). 与传统的摄像器件相比,线阵CCD21具有光谱响应宽、线性好、动态范围宽、噪声低、灵敏度高、实时传输和电荷自扫描等多方面的优点,目前己广泛应用于遥感成像、卫星监测、 机器视觉等领域。 Compared with the conventional imaging device having a linear array CCD21 wide spectral response, good linearity, wide dynamic range, low noise, high sensitivity, and real-time transmission of charge from many advantages scanning, sensing imaging has been widely used at present, the satellite monitoring, machine vision and other fields.

[0039] 本发明公开的一种光谱编码共焦与光学相干层析协同成像方法与系统,提出的将光学相干层析协同(0CT)成像系统的样品臂与光谱编码共焦(SECM)系统的样品臂共用同一光路,使得样品臂中从0CT系统与SECM系统出射的光束对样品同一位置同步进行扫描,并且从样品单次散射返回的光分别返回各自的光路返回探测臂,这种光学耦合的方法有效克服了将两系统结合时使用机械切换所带来的问题,真正做到了同步扫描样品的同一位置。 [0039] The present invention discloses a spectroscopic encoding the sample arm confocal spectral optical coherence tomography cooperative coding (0CT) confocal imaging system (the SECM) cooperative with optical coherence tomography imaging method and system of the proposed system sample arm share the same optical path, such that the sample arm from 0CT system SECM system beam emitted to the same position of the sample simultaneously scanned, and return light from the sample single scattering returned each respective optical path to return the probe arm, such an optical coupling the method overcomes the problems of the effective mechanical bonding two switched system brought truly synchronous scanning the same position of the sample. 这种光谱编码共焦与光学相干层析协同成像方法与系统,使得该系统的轴向分辨率相对于SECM系统有了很大提高,并且使其横向分辨率相对于0CT系统得到很大提高,优化了系统的成像质量。 Such coding spectral optical coherence tomography and confocal imaging methods and systems synergy, such that axial resolution of the system with respect to the SECM system has been greatly improved, with respect to the lateral resolution and allowed 0CT system has been greatly improved, optimize the image quality of the system. 另外,相比之前的方法和系统,本发明中的样品臂的光路更加简单,便于实施,并且只需要采用一个光源,节约了成本并简化了系统结构,光路也更加稳定。 Further, compared to the previous methods and systems, the sample arm optical path in the present invention is more simple, easy to implement, and requires only one light source is employed, cost savings and simplifies the system structure, the optical path is also more stable. 提出的探测臂采用基于两个光纤准直镜以不同入射角照射光栅,使光栅衍射出两组无交叠出射的光谱信号,即为SECM和OCT的信号,经过聚焦镜头,分别聚焦于线阵电荷耦合器件(CCD)的左半部分像素和右半部分像素,即实现同时探测来自SECM和OCT系统的光谱信号。 Proposed two probe arm based fiber collimator lens irradiating the grating at different incident angles, so that the grating diffraction spectrum of the two non-overlapping signals emitted, i.e. SECM and OCT signal, through the focusing lens, are focused on linear a charge coupled device (CCD) pixel and the left half right half pixels, i.e. achieve simultaneous detection signal from the SECM and spectral OCT system. 经传入计算机进行数据处理获取被测样品高轴向分辨率的OCT图像和高横向分辨率的SECM图像,实现OCT系统与SECM系统优势互补的协同成像。 Data processing by a computer to obtain a test sample the incoming high-resolution OCT image and an axial image SECM high lateral resolution, OCT system and achieve complementary synergistic SECM imaging system. 这种探测方法省去了同时使用两个光谱仪探测信号的复杂结构,只需要用一个探测臂探测SECM系统和OCT系统的光谱信号,使得系统结构更加紧凑,光路更为稳定,同时也节约了搭建成本,并使探测臂中的光栅、凸透镜和线阵CCD得到了充分的利用。 This eliminates the need for complex structure detection method using both detection signals of the spectrometer, a spectral signal only need arm detecting SECM detection system and the OCT system, making the system more compact, more stable optical path, but also saves building cost, and the grating in the detection arm, a convex lens and linear CCD has been fully utilized. 此外,两系统的成像结果相互配准、像素对齐,使系统的成像结果更加精确。 Further, results of the two imaging systems each registration, the pixel alignment of the imaging system is more accurate results. 这种光谱编码共焦与光学相干层析协同成像方法与系统的以上优势在生物医学成像以及材料检测等应用领域都有着重要的意义。 This spectrum coding confocal optical coherence with the above image forming method synergistic advantages chromatography system with applications in biomedical imaging and material testing are of great significance.

[0040] 以上所述仅是本发明的优选实施方式,应当指出:对于本技术领域的普通技术人员来说,在不脱离本发明原理的前提下,还可以做出若千改进和润饰,这些改进和润饰也应视为本发明的保护范围。 [0040] The above are only preferred embodiments of the present invention, it should be noted: to those of ordinary skill in the art, in the present invention without departing from the principles of the premise, may also be made if one thousand improvements and modifications of these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims (3)

  1. 1. 一种光谱编码共焦与光学相干层析协同成像系统,其特征在于:包括光源(1)、参考臂(2)、样品臂(3)、探测臂⑷、传输光纤(5)、第一光隔离器(6)、第二光隔离器(7)、第一光纤耦合器⑻和第二光纤耦合器(9),所述第一光纤耦合器⑻的一端分别通过传输光纤(5) 分别连接光源⑴和探测臂⑷,而另一端通过传输光纤⑸分别连接样品臂⑶和第二光纤耦合器(9)的一端,且所述光源⑴与第一光纤耦合器⑻之间设置有第一光隔离器(6),第一光纤耦合器(8)和第二光纤耦合器(9)之间接有第二光隔离器(7);所述第二光纤耦合器0?)与第一光纤耦合器⑻连接的一端还与探测臂⑷连接,而所述第二光纤耦合器C?)的另一端分别连接参考臂(2)和样品臂(3);所述样品臂(3)包括按光速流向依次设置的第一光纤准直镜(10)、第一光栅(11)、第一凸透镜(12)、第二凸透镜(13)以及显微物镜( A coding spectral optical coherence tomography and confocal imaging collaborative system comprising: a light source (1), the reference arm (2), the sample arm (3), probe arm ⑷, transmission fiber (5), an optical isolator (6), a second optical isolator (7), a first and a second fiber coupler ⑻ fiber coupler (9), one end of said first fiber coupler via the transmission optical fiber respectively ⑻ (5) respectively connecting the light source and the probe arm ⑴ ⑷, and the other end connected to one end of the sample arm ⑶ and second fiber coupler (9) by a transmission fiber ⑸ respectively, disposed between the light source and the first optical coupler and ⑴ ⑻ subsection an optical isolator (6), a first fiber coupler (8) and a second fiber coupler (9) has a second indirect optical isolator (7); the second fiber coupler 0) and the first? ? ⑻ connection end of the fiber coupler is also connected to the probe arm ⑷, and the second optical fiber coupler C) respectively connected to the other end of the reference arm (2) and the sample arm (3); the sample arm (3) comprises flow speed of light are sequentially arranged according to a first optical fiber collimator lens (10), a first grating (11), a first convex lens (12), a second lens (13) and microscope objective ( 16),所迷第一光纤耦合器(8)和第二光纤耦合器(9)连接的一端与第一光纤准直镜(10)相连,且所述第一光栅(11)的光斑中心、第一凸透镜(12)中心、第二凸透镜(13)中心以及显微物镜中心在同一条光轴上;以第一凸透镜(12)与第二凸透镜(13)光轴连线方向为Z轴,以第二光纤准直镜(14)和振镜(15)的光轴连线方向为Y轴,以第一凸透镜的中心为原点,根据右手定则, 建立坐标系;所述第一光栅(11)表面垂直于YZ平面,且所述第一光栅(11)与XZ的夹角为a; 还包括按光束传播顺序依次设置的第二光纤准直镜(14)和振镜(15),所述第二光纤耦合器(9) 和参考臂(2)连接的一端与第二光纤准直镜(14)连接,所述振镜(15)设置于第一光栅(11)与第一凸透镜(12)之间,所述振镜(15)表面垂直于YZ平面,且振镜(15)与第二光纤准直镜(14)的高度在同一水平面,另外所述第二光纤 16), the fans of the first fiber coupler (8) and a second fiber coupler (9) is connected to one end of a first optical fiber connected to the collimator lens (10), and said first grating (11) in the center of the spot, a first convex lens (12) center, a second lens (13) and the center of a microscope objective centered on the same optical axis; a first convex lens (12) and a second convex lens (13) connecting the optical axis direction of the Z-axis, a second optical fiber collimator lens (14) and a galvanometer (15) connecting the optical axis direction is the Y axis, a first lens center as the origin, according to the right hand rule coordinate system is established; the first grating ( 11) surface perpendicular to the YZ plane, and the first grating (11) for the XZ angle a; a second optical fiber further comprises a collimator lens according to the beam propagation sequentially arranged sequentially (14) and a galvanometer (15), One end of the second optical fiber collimator lens (14) of said second optical coupler (9) and a reference arm (2) connected to the connector, the galvanometer (15) disposed on the first grating (11) and the first convex lens (12), said galvanometer (15) surface perpendicular to the YZ plane, and the oscillating mirror (15) and the height of the second optical fiber collimator lens (14) is at the same level, while the second optical fiber 准直镜(14)和振镜(15)在第一光栅(11) 上的光斑下方沿正X轴方向的偏移量>0;所述第一光纤准直镜(10)将来自第一光纤耦合器(8)的光束投射到第一光栅(11)表面上形成光斑,从第一光栅衍射出的光谱投射在第一凸透镜(12)的上半部分;第二光纤准直镜(14)将来自第二光纤耦合器(9)的光束投射在振镜(15)上,该光束经过振镜(15)反射在第一凸透镜(12)的下半部分,通过调整第一光栅(11) 放置角度a和振镜(15)的扫描范围使得从振镜(15)反射的光束的扫描角范围与第一光栅(11) 出射光的角色散范围相等;投射在第一凸透镜(12)的上下两部分光束经过第一凸透镜(12) 后,在第一凸透镜(12)的像方焦平面聚焦成同一条水平方向的亮线斑,这条亮线斑在中点处与第一凸透镜(12)的光轴垂直正交,并继续入射到第二凸透镜(13)上;从第二凸透镜(13)出射的光束得到会聚 Collimator lens (14) and a galvanometer (15) offset in the positive X-axis direction below the spot (11) in the first grating> 0; the first optical fiber collimator lens (10) from the first beam fiber coupler (8) striking the first grating (11) forming a light spot on the surface of the diffraction grating from the first half of the spectrum is projected on a first convex lens (12); a second optical fiber collimator lens (14 ) projects a light beam from the second fiber coupler (9) in a galvanometer (15), through the galvanometer beam (15) reflected in the lower half of the first convex lens (12) is divided, by adjusting the first grating (11 ) and a placement angle of the galvanometer (15) from the sweep range such that the galvanometer (15) and the scan angle range of the first grating (11) the reflected beam is equal to the angular dispersion range of the emitted light; a first projection lens (12) the upper and lower halves after the beam passes through a first convex lens (12), a first convex lens (12) is focused into the same focal plane as a horizontal bright line spots, this bright line at the midpoint of the spot of the first lens (12) is perpendicular to the optical axis and incident on the second lens continues (13); obtained from the second condensing lens (13) beam exiting 并全部入射到显微物镜(16)中,经过显微物镜(16)聚焦的光束投射在样品上;从样品单次散射返回的光经原光路返回并分别经过第一光纤准直镜(10) 、第二光纤准直镜(14)返回各自的光路,并进入探测臂;所述探测臂(4)包括第三光纤准直镜(17)、第四光纤准直镜(18)、第二光栅(I9)、聚焦镜头(20)和线阵电荷耦合器件(21);所述第一光纤耦合器(S)和光源(1)连接的一端与第四光纤准直镜(1S)连接,而所述第二光纤耦合器(9)和第一光纤耦合器⑻连接的一端与第三光纤准直镜(17)连接;所述第三光纤准直镜(17)和第四光纤准直镜(18)在第二光栅(19)的同一侧,且所述第三光纤准直镜(I7)和第四光纤准直镜(1S)分别与第二光栅(I9)成不同的夹角放置;而所述第二光栅(19)另一侧放置线阵电荷耦合器件(21),所述聚焦镜头(2〇)放置在第二光栅(19)与线阵电荷耦合器件(21) And all incident to the microscope objective (16), through the microscope objective (16) projecting a light beam focused on the sample; optical path of the original light is scattered back from the sample and a single return optical fiber respectively through a first collimating lens (10 ), a second optical fiber collimator lens (14) return to their respective optical paths, and into the probe arm; the detection arm (4) comprises a third optical fiber collimator lens (17), a fourth optical fiber collimator lens (18), the first second raster (I9), focusing lens (20) and a charge coupled device linear array (21); said first coupler fiber (S) and the light source (1) connected to one end of the fourth optical fiber collimator lens (1S) connected and said second optical coupler (9) and an end of a first fiber coupler ⑻ connected to the third optical fiber collimator lens (17); said third optical fiber collimator lens (17) and a fourth fiber collimator collimator lens (18) on the same side of the second grating (19), and said third optical fiber collimator lens (I7) and the fourth optical fiber collimator lens (1S) respectively and a second grating (I9) into different folders placed at an angle; and said second grating (19) disposed on the other side a linear array charge coupled device (21), said focusing lens (2〇) placed in a second grating (19) with a charge coupled device linear array (21) 之间;所述第三光纤准直镜(17)收集来自于第一光纤耦合器(8)的光束,而所述第四光纤准直镜(18)收集来自于第二光纤耦合器(9)的光束,且所述第三光纤准直镜(1乃、第四光纤准直镜(1S)分别以不同的入射角度将两个光束投射到第二光栅(19)上,使第二光栅(19)衍射出两组无交叠出射的光谱信号,即为SECM和0CT的信号,经过聚焦镜头(20),分别聚焦于线阵电荷耦合器件(21)光敏面的左半部分像素和右半部分像素。 Between; the third optical fiber collimator lens (17) collects the light beam from the first fiber coupler (8), and said fourth optical fiber collimator lens (18) collected from the second fiber coupler (9 ) beam, and the third optical fiber collimator (1 is the fourth optical fiber collimator lens (1S) respectively to two different angles of incidence of the light beam projected onto the second grating (19), the second grating (19) the diffraction spectrum of the two non-overlapping signals emitted, and is the SECM 0CT signal, through the focusing lens (20), are focused on the right and the left half of the pixel surface of the photosensitive charge coupled device linear array (21) half of the pixels.
  2. 2.根据权利要求1所述的光谱编码共焦与光学相干层析协同成像系统,其特征在于:所述第一凸透镜(12)与第二凸透镜(13)之间的距离为两透镜的焦距之和,第二凸透镜(13)与显微物镜(16)的距离等于第二凸透镜(13)的焦距。 The spectral confocal optical encoder according to claim 1 OCT imaging system cooperative, wherein: a distance between the first lens (12) and a second convex lens (13) is a focal length of the two lenses and the distance, a second lens (13) and microscope objective (16) is equal to the second convex lens (13) focal length.
  3. 3.根据权利要求2所述的光谱编码共焦与光学相千层析协同成像系统,其特征在于:所述两组光谱信号无交叠地聚焦在线阵电荷耦合器件(21)光敏面上。 3. The spectral confocal optical encoder according to claim 2 with one thousand chromatography synergistic imaging system, characterized in that: said two non-overlapping spectral signal line focus charge coupled device array (21) of the photosensitive surface.
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