CN104223123A - Preparation method of probiotic-rich pleurotus eryngii dietary fiber - Google Patents
Preparation method of probiotic-rich pleurotus eryngii dietary fiber Download PDFInfo
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- CN104223123A CN104223123A CN201410486314.7A CN201410486314A CN104223123A CN 104223123 A CN104223123 A CN 104223123A CN 201410486314 A CN201410486314 A CN 201410486314A CN 104223123 A CN104223123 A CN 104223123A
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- 244000252132 Pleurotus eryngii Species 0.000 title claims abstract description 54
- 235000001681 Pleurotus eryngii Nutrition 0.000 title claims abstract description 54
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- 239000006041 probiotic Substances 0.000 title claims abstract description 9
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 9
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention discloses a preparation method of pleurotus eryngii dietary fiber, and belongs to the technical field of deep processing of agricultural products. The preparation method of the pleurotus eryngii dietary fiber comprises the following steps: soaking pleurotus eryngii residues separated out after polysaccharide extraction into edible alcohol, filtering to remove proteins, washing the pleurotus eryngii residues with water, adding inorganic alkali, heating for hydrolysis, washing the pleurotus eryngii residues to be neutral after alkaline hydrolysis, adding inorganic acid, heating for hydrolysis, washing the pleurotus eryngii residues with water and filtering after acid hydrolysis, drying, crushing, adding a well-cultured probiotic culture solution, and performing low-temperature spray drying to obtain the probiotic-rich pleurotus eryngii dietary fiber. In the preparation method, the pleurotus eryngii residues separated out after polysaccharide extraction are made full use for preparing the probiotic-rich pleurotus eryngii dietary fiber; the preparation method is simple and reasonable in process route; an acid method and an alkali method are combined, so that the environment is not polluted; the preparation method is convenient in operation and low in production cost.
Description
Technical field
The invention belongs to agricultural product intensive processing technical field, relate to a kind of preparation method being rich in the pleurotus eryngii diet fiber health-care food of probio.
Background technology
Dietary fiber (dietary fiber, DF) typically refer to and can digest and assimilate by human body small intestine, and the summation of edible vegetable ingredients, carbohydrate and the similar substance thereof that can partly or entirely ferment at human body large intestine, comprise polysaccharide, oligosaccharides, lignin and other plant associated matter that a part can not be digested.Water-soluble dietary fiber (soluble dietary fiber can be divided into according to DF dissolution characteristics, and water insoluble dietary fiber (insoluble dietary fiber SDF), IDF), DF mainly comprises cellulose, hemicellulose, lignin etc.Although DF can not be digested, but large quantity research shows, take in the DF of q.s for balancing nutrients in body, regulate human body physiological function, prevent and treat the various diseases such as coronary heart disease, diabetes important role, thus DF is listed in " the seventh-largest nutrient " after protein, fat, carbohydrate, vitamin, mineral matter and water.Pleurotus eryngii (Pleurotus eryngii) is also known as pleurotus eryngii, and its fructification color and luster is snow-white, and quality is tender and crisp.Pleurotus eryngii is rich in fiber, and dry weight content reaches 8.5%, is only second to the asparagus that fiber content is the highest, and is rich in various mineral salt, is the good raw material that exploitation has the dietary fiber of health care.The extracting method of dietary fiber and the composition of raw material and character closely related, have chemical method, enzyme process and membrane separation process etc.Chemical Decomposition dietary fiber mainly contains direct water extraction, acid-base method and flocculant method etc.Although chemical method is simple and easy to do, equipment investment is few, suitability for scale production.But be used alone acid system or alkaline process, easily produce the disagreeableness sewage of environment.Membrane separation process is by changing the molecule interception of film, highly purified dietary fiber can be extracted for separating oligomeric sugar with some micromolecular acid, enzyme, or prepare the dietary fiber of different molecular weight, and can suitability for industrialized production be realized, can predict it will be the yield and the most promising method of separating water-soluble dietary fiber that improve insoluble diedairy fiber, but the method is also immature at present, cannot be used widely.
Probio (Probiotics) is that a class can be colonizated in digestion and improves human body microecological balance, the microorganism general name useful to health.Probio can be divided into three major types: (1) lactobacillus class, comprises lactobacillus acidophilus, Lactobacillus casei, Lactobacillus Jensenii, Raman lactobacillus etc.; (2) Bifidobacterium class, comprises bifidobacterium longum, bifidobacterium breve, avette Bifidobacterium, bifidobacterium thermophilum etc.; (3) gram-positive cocci, comprises bacillus coagulans, streptococcus fecalis, galactococcus, intermediary streptococcus etc.Probio has prevention or improvement diarrhoea, alleviates lactase deficiency shape, strengthens body immunity, promotes enteron aisle digestive system health and reduce the effects such as serum cholesterol.In addition, long-term probiotic supplemented also contribute to prevent bone loss, prevention of osteoporosis disease.
Further, the healthy food also still not having a kind of probio to be combined with edible fungus dietary fiber at present.
Summary of the invention
An object of the present invention is the above-mentioned defect overcome existing for prior art, provides a kind of pleurotus eryngii dietary fiber being rich in probio.
The technical solution used in the present invention is as follows:
Be rich in a pleurotus eryngii dietary fiber for probio, it is characterized in that its preparation method comprises following steps:
(1) getting the pleurotus eryngii slag after extracting polysaccharide, is the edible alcohol of 75-95% by concentration, and 1-3 hour is stirred on immersion limit, limit, soaks and terminates rear 200-250 eye mesh screen filtration removal albumen;
(2) the mushroom slag after step (1) being removed albumen is transferred in hydrolytic decomposition pot, adds water and does not have, and then add the inorganic alkali solution of 3 moles often liter, regulate pH to 7.0-10.5, be warming up to 50-65 DEG C, basic hydrolysis 1-5 hour, basic hydrolysis terminates rear filtration, and is washed to neutrality;
(3) the mushroom slag after step (2) basic hydrolysis is dropped in hydrolytic decomposition pot, add water and do not have, and then add the acid solution of 2 moles often liter, regulate pH to 2.0-6.5, be warming up to 40-55 DEG C, acid hydrolysis 2-8 hour, then filter, and be washed to neutrality, obtained dietary fiber crude product;
(4) the dietary fiber crude product that step (3) obtains is carried out drying under 55 DEG C of-90 DEG C of temperature conditions, cross 300-350 eye mesh screen after crushed after drying, obtain water-insoluble pleurotus eryngii dietary fiber;
(5) step (4) obtain dietary fiber in add conventional method cultivate probiotic's culture liquid, make solid content more than 15%, probio content is at 105/more than mL;
(6) step (5) is obtained containing bacterium dietary fiber slurries, under 70-80 DEG C of condition, centrifugal spray drying, namely obtains the pleurotus eryngii dietary fiber being rich in probio, and this dietary fiber can be placed in 4-15 DEG C of shady and cool place and preserve.
In above-mentioned steps (2), said inorganic base can be NaOH or potassium hydroxide, or ammoniacal liquor.
In above-mentioned steps (3), said acid can be hydrochloric acid or sulfuric acid, or phosphoric acid, or acetic acid.
In above-mentioned steps (5), said probio is one or more in Bifidobacterium, bacillus coagulans and lactic acid bacteria.
Two of object of the present invention is to provide a kind of pleurotus eryngii dietary fiber being rich in probio and is preparing the purposes in treatment and health food.
Preferably, health food described above is for preventing and treating coronary heart disease, and diabetes, improve body immunity, promotes that enteron aisle digestive system is healthy, reduces serum cholesterol, prevent bone loss, the health food of prevention of osteoporosis disease aspect.
The present invention compared with prior art has following progressive or unexpected technique effect.
The inventive method makes full use of the pleurotus eryngii dietary fiber that probio is rich in the preparation of the pleurotus eryngii slag after extracting polysaccharide, its process route advantages of simple, acid system and alkaline process is taked to be combined, the waste water produced discharges after can carry out neutralization precipitation, do not pollute the environment, influence factor is few, easy to operate, and production cost is low.In addition, clinical trial shows, and the pleurotus eryngii dietary fiber of prepared by the inventive method be rich in probio, can be used for coronary heart disease, diabetes supplemental treatment, and coronary heart disease, diabetic complication recovers, and keep blood glucose balance, daily health caring etc. have good effect.
Detailed description of the invention
Form is described in further detail foregoing of the present invention again by the following examples, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to following embodiment, all technology realized based on foregoing of the present invention all belong to scope of the present invention.
Embodiment 1
(1) take 100kg and extract the pleurotus eryngii slag after polysaccharide, add 200L, concentration is the edible alcohol of 75%, immersion limit, limit stirs 1 hour, soaks to terminate rear selection 250 eye mesh screen and cross and filter albumen;
(2) by step (1) except the mushroom slag after deproteinized is transferred in hydrolytic decomposition pot, add water 200L, mushroom slag was not had, and then add the sodium hydroxide solution of 3 moles often liter, regulate pH to 8.5, be warming up to 60 DEG C, basic hydrolysis 3 hours, basic hydrolysis terminates rear filtration, and is washed to neutrality;
(3) will drop in hydrolytic decomposition pot through the mushroom slag of step (2) basic hydrolysis, the 300L that adds water did not have, and then add the hydrochloric acid solution of 2 moles often liter, regulate pH to 3.5, be warming up to 50 DEG C, acid hydrolysis 4 hours, acid hydrolysis terminates rear filtration, and be washed to neutrality, obtain dietary fiber crude product; And acidolysis waste water and alkaline hydrolysis waste water are carried out ratio mix, obtain neutral water, drain in sedimentation basin and precipitate;
(4) the dietary fiber crude product of above-mentioned acquisition is carried out drying, baking temperature 80 DEG C, cross 300 eye mesh screens after crushed after drying, obtain water-insoluble pleurotus eryngii dietary fiber, weigh to obtain 680g;
(5) mixed with the dietary fiber that step (4) obtains by the bacillus coagulans nutrient solution that conventional method is cultivated, join to obtain suspension slurry 3L, make solid content substantially 20%, probio content is 5 × 10
5individual/mL;
(6) what step (5) obtained contains bacterium slurries under 75 DEG C of conditions, and namely centrifugal spray drying obtains the pleurotus eryngii dietary fiber being rich in probio, is weighed as 720g, and can put 4 DEG C of shady and cool place preservations.
Embodiment 2
(1) take 150kg and extract the pleurotus eryngii slag after polysaccharide, add 300L, concentration is the edible alcohol of 95%, immersion limit, limit stirs 1 hour, soaks to terminate rear selection 250 eye mesh screen and cross and filter albumen;
(2) step (1) is transferred in hydrolytic decomposition pot except the mushroom slag after deproteinized, adds water 300L, do not have mushroom slag, and then add the potassium hydroxide solution of 3 moles often liter, regulate pH to 7.0, be warming up to 50 DEG C, basic hydrolysis 5 hours, basic hydrolysis terminates to filter, and is washed to neutrality;
(3) the mushroom slag of step (2) basic hydrolysis is dropped in hydrolytic decomposition pot, add water 450L not have, and then add the sulfuric acid solution of 2 moles often liter, regulate pH to 2.0, be warming up to 40 DEG C, acid hydrolysis 2 hours, acid hydrolysis terminates to filter, and be washed to neutrality, obtain pleurotus eryngii dietary fiber crude product; Acidolysis water wasting and alkaline hydrolysis waste water can be carried out ratio to mix simultaneously, obtain neutral water, drain in sedimentation basin and precipitate;
(4) the dietary fiber crude product that above-mentioned steps (3) obtains is carried out drying, baking temperature 55 DEG C, through pulverizing 300 eye mesh screens after drying, obtaining water-insoluble pleurotus eryngii dietary fiber, being weighed as 1130g;
(5) mixed with the dietary fiber of step (4) by the probiotic Bifidobacterium longum nutrient solution that conventional method is cultivated, join to obtain suspension slurry 6L, make solid content substantially 16%, probio content is 3 × 10
5individual/mL;
(6) by step (5) containing bacterium slurries under 70 DEG C of conditions, centrifugal spray drying, namely obtain and be rich in the pleurotus eryngii dietary fiber of probio, weigh about 1280g, and can be placed in 4 DEG C of shady and cool places and preserve.
Embodiment 3
(1) take the pleurotus eryngii slag after 100kg extraction polysaccharide, be the edible alcohol of 90% by 200L concentration, immersion limit, limit stirs 3 hours, soaks and terminates rear selection 250 eye mesh screen filtration removal albumen;
(2) step (1) is transferred in hydrolytic decomposition pot except the mushroom slag after deproteinized, adds water 200L, not mistake, and then add the ammoniacal liquor of 3 moles often liter, regulate pH to 10.0, be warming up to 65 DEG C, basic hydrolysis 1 hour, basic hydrolysis terminates to filter, and is washed to neutrality;
(3) the mushroom slag of step (2) basic hydrolysis is dropped in hydrolytic decomposition pot, add water 300L not have, and then add the phosphoric acid solution of 2 moles often liter, regulate pH to 6.0, be warming up to 55 DEG C, acid hydrolysis 8 hours, acid hydrolysis terminates rear filtration, and be washed to neutrality, obtain pleurotus eryngii dietary fiber crude product; Acid is carried out ratio mixing in conjunction with the waste water of alkaline hydrolysis simultaneously, obtain neutral water, drain in sedimentation basin and precipitate;
(4) the dietary fiber crude product that above-mentioned steps (3) obtains is carried out drying, baking temperature 90 DEG C, cross 300 eye mesh screens after crushed after drying, obtain water-insoluble pleurotus eryngii dietary fiber, weigh to obtain 740g;
(5) bifidobacterium longum conventional method cultivated, bacillus coagulans and lactic acid bacteria culture solution add in the dietary fiber of step (4) and mix, and join to obtain suspension slurry 3.2L, make solid content about 20%, probio content is 3 × 10
6individual/mL;
(6) by step (5) containing bacterium slurries under 80 DEG C of conditions, centrifugal spray drying obtains and is rich in the pleurotus eryngii dietary fiber of probio, and weigh to obtain 745g, puts 4 DEG C of shady and cool places and preserves.
Embodiment 4
(1) take the pleurotus eryngii slag after 200kg extraction polysaccharide, add the edible alcohol that 400L concentration is 80%, immersion limit, limit stirs 2 hours, soaks and terminates the filtration of rear selection 250 eye mesh screen, remove albumen;
(2) be transferred in hydrolytic decomposition pot by the mushroom slag after deproteinized, add water 400L and do not have, and then add the sodium hydroxide solution of 3 moles often liter, regulate pH to 9.5, be warming up to 60 DEG C, basic hydrolysis 2 hours, basic hydrolysis terminates rear filtration, and is washed to neutrality;
(3) the mushroom slag of step (2) basic hydrolysis is dropped in hydrolytic decomposition pot, add water 600L not have, then the hydrochloric acid solution of 2 moles often liter is added, regulate pH to 3.0, be warming up to 55 DEG C, acid hydrolysis 3 hours, acid hydrolysis terminates to receive filters, and be washed to neutrality, obtain pleurotus eryngii dietary fiber crude product; Acid is carried out ratio mixing in conjunction with the waste water of alkaline hydrolysis simultaneously, obtain neutral water, drain in sedimentation basin and precipitate;
(4) the dietary fiber crude product that step (3) obtains is carried out drying, baking temperature 85 DEG C, through pulverizing 300 eye mesh screens after drying, obtain water-insoluble pleurotus eryngii dietary fiber, weigh to obtain 1722g;
(5) mixed with the dietary fiber of step (4) by bifidobacterium longum/bacillus coagulans nutrient solution that conventional method is cultivated, join to obtain suspension slurry 13.5L, make solid content about 18%, probio content is 2 × 10
5individual/mL;
(6) by step (5) containing bacterium slurries under 75 DEG C of conditions, centrifugal spray drying, namely obtain and be rich in the pleurotus eryngii dietary fiber of probio, weigh to obtain 1984g, and juxtaposition 4 DEG C cool place place preserves.
5 diabetic's clinical testings
5.1. clinical data
Choose 126 routine diabetics altogether for treatment target, be divided into A, B, C tri-groups at random, often organize 42 people.Wherein developed complications shape person 36 people, often organizes Random assignment 12 people.
5.2. methods for the treatment of
A group: according to therapeutic dose injection insulin glargine, 1 times/day, as reference group;
B group: according to therapeutic dose injection insulin glargine, 1 times/day, add the pleurotus eryngii dietary fiber being rich in probio prepared by 6 grams of the inventive method in addition every day in meals.
C group: injection insulin glargine, 1 times/day, each one; Pleurotus eryngii dietary fiber prepared by 6 grams of existing chemical methods is added in addition every day in meals.
5.33. efficacy assessment standard
(1), at injectable drug before 1 day, after injectable drug the 60th day, fasting blood-glucose index is detected respectively to often organizing; All test datas mean ± standard deviation represents.Carry out statistical disposition with SPSS13.0 statistical software, all homogeneity test of variance is carried out to each group of data, use one-way analysis of variance between different group when variance is neat, compare between two and check with SNKq; Heterogeneity of variance K-WH checks, and compares between two and compares between two by Neminy method.Inspection level=0.05.
5.44. treatment results is as follows:
The comparison (mmol/L) of blood sugar before and after table 1 three groups of patient on medication
| Group | Before medication | After medication 60 days |
| A group | 11.6±0.6 | 7.8±0.4 |
| B group | 11.5±0.7 | 5.9±0.4*# |
| C group | 11.6±0.8 | 7.6±0.8 |
Note: compare with A group, * p<0.05; Compare with B group, #p<0.05.
The situation of medication A, B, C3 group complication patient after 60 days all takes a turn for the better, the blood sugar of the patient of 3 groups all obviously reduces, B group hypoglycemic effect is best, all there is significant difference compared with A group or B group, the patient of the concurrent symptom of becoming thin of B group diabetes in patients, body weight obviously increases, and complexion is better, and health feels best.The pleurotus eryngii dietary fiber of probio is rich in visible the present invention, and to the recovery of diabetic, daily health caring has good adjuvant treatment effect.
Claims (7)
1. be rich in a pleurotus eryngii dietary fiber for probio, it is characterized in that, its preparation method comprises following steps:
(1) getting the pleurotus eryngii slag after extracting polysaccharide, is the edible alcohol of 75-95% by concentration, soaking and stirring 1-3 hour, soaks and terminates the filtration of rear 200-250 eye mesh screen, remove albumen;
(2) the pleurotus eryngii slag after step (1) being removed albumen is transferred in hydrolytic decomposition pot, adds water and does not have, then add the inorganic alkali solution of 3M, regulate pH to 7.0-10.5, be warming up to 50-65 DEG C, basic hydrolysis 1-5 hour, basic hydrolysis terminates rear filtration, is washed to neutrality;
(3) the mushroom slag after step (2) basic hydrolysis is dropped in hydrolytic decomposition pot, add water and do not have, then add the acid solution of 2M, regulate pH to 2.0-6.5, be warming up to 40-55 DEG C, acid hydrolysis 2-8 hour, filter, be washed to neutrality, obtained dietary fiber crude product;
(4) the dietary fiber crude product that step (3) obtains is carried out drying under 55 DEG C of-90 DEG C of temperature conditions, cross 300-350 eye mesh screen after crushed after drying, obtain water-insoluble pleurotus eryngii dietary fiber;
(5) step (4) obtain dietary fiber in add conventional method cultivate probiotic's culture liquid, make solid content more than 15%, probio content is 10
5individual/more than mL;
(6) step (5) is obtained containing bacterium dietary fiber slurries, under 70-80 DEG C of condition, centrifugal spray drying, namely obtains the pleurotus eryngii dietary fiber being rich in probio.
2. be rich in the pleurotus eryngii dietary fiber of probio as claimed in claim 1, it is characterized in that: described in preparation method's step (1), the concentration of edible alcohol is 80-90%.
3. be rich in the pleurotus eryngii dietary fiber of probio as claimed in claim 1, it is characterized in that: the inorganic base described in preparation method's step (2) is NaOH, or potassium hydroxide, or ammoniacal liquor.
4. be rich in the pleurotus eryngii dietary fiber of probio as claimed in claim 1, it is characterized in that: the acid described in preparation method's step (3) is hydrochloric acid, or sulfuric acid, or phosphoric acid, or acetic acid.
5. be rich in the pleurotus eryngii dietary fiber of probio as claimed in claim 1, it is characterized in that: the probio described in preparation method's step (5) is one or more in Bifidobacterium, bacillus coagulans, lactic acid bacteria.
6. the pleurotus eryngii dietary fiber being rich in probio as described in as arbitrary in claim 1-5 is preparing the purposes in treatment and health food.
7. be rich in the pleurotus eryngii dietary fiber of probio as claimed in claim 6, it is characterized in that: described health food is for preventing and treating coronary heart disease, diabetes, improve body immunity, promote that enteron aisle digestive system is healthy, reduce serum cholesterol, prevent bone loss, the health food of prevention of osteoporosis disease aspect.
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