CN1483349A - Method for preparing soluble dietary fibre - Google Patents
Method for preparing soluble dietary fibre Download PDFInfo
- Publication number
- CN1483349A CN1483349A CNA021330123A CN02133012A CN1483349A CN 1483349 A CN1483349 A CN 1483349A CN A021330123 A CNA021330123 A CN A021330123A CN 02133012 A CN02133012 A CN 02133012A CN 1483349 A CN1483349 A CN 1483349A
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- Prior art keywords
- dietary fiber
- adds
- cereal kind
- soluble
- kind skin
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- 235000013325 dietary fiber Nutrition 0.000 title claims abstract description 29
- 238000000034 method Methods 0.000 title claims abstract description 10
- 229920002472 Starch Polymers 0.000 claims abstract description 7
- 229920002678 cellulose Polymers 0.000 claims abstract description 7
- 239000001913 cellulose Substances 0.000 claims abstract description 7
- 239000008107 starch Substances 0.000 claims abstract description 7
- 235000019698 starch Nutrition 0.000 claims abstract description 7
- 229920001221 xylan Polymers 0.000 claims abstract description 7
- 150000004823 xylans Chemical class 0.000 claims abstract description 7
- 108010059892 Cellulase Proteins 0.000 claims abstract description 4
- 108091005804 Peptidases Proteins 0.000 claims abstract description 4
- 229940106157 cellulase Drugs 0.000 claims abstract description 4
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 4
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000013339 cereals Nutrition 0.000 claims description 15
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 229940088598 enzyme Drugs 0.000 claims description 11
- 240000008042 Zea mays Species 0.000 claims description 8
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 8
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 8
- 235000009973 maize Nutrition 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 3
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims description 3
- 230000029087 digestion Effects 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 3
- 239000002994 raw material Substances 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 238000000108 ultra-filtration Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 235000013527 bean curd Nutrition 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 230000007062 hydrolysis Effects 0.000 claims description 2
- 238000006460 hydrolysis reaction Methods 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 5
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 4
- 108010028554 LDL Cholesterol Proteins 0.000 abstract description 2
- 238000003756 stirring Methods 0.000 abstract 2
- 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 abstract 1
- 102000035195 Peptidases Human genes 0.000 abstract 1
- 235000012000 cholesterol Nutrition 0.000 abstract 1
- 229940111205 diastase Drugs 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 108010022197 lipoprotein cholesterol Proteins 0.000 abstract 1
- 235000019833 protease Nutrition 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 238000010025 steaming Methods 0.000 abstract 1
- 239000000835 fiber Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 230000035790 physiological processes and functions Effects 0.000 description 4
- 239000000126 substance Substances 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 239000002778 food additive Substances 0.000 description 1
- 235000013373 food additive Nutrition 0.000 description 1
- 239000005417 food ingredient Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Chemical class 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The method for preparing soluble dietary fibre includes the following steps: pulverizing grain seed cover, adding water, heating, steaming and softening, then adding alpha-diastase, stirring and removing the contained starch, then adding proteinase, heat-insulating and stirring, removing the contained protein, adding xylanase and cellulase produced by mould, enzymolysis, hydrolyzing the cellulose and xylan being in the grain seed into soluble cellulose and xylan so as to obtain the invented soluble dietary fibre with good effect or reducing blood-lipid level, serium total cholesterol and low-density lipoprotein cholesterol level.
Description
Technical field
The invention belongs to the production method of food additives.
Background technology
Dietary fiber (Dietary Fiber is called for short DF) means the general name that is difficult to by the high molecular polysaccharide material of enzymic digestion in human body.Dietary fiber has the important physical function to human body, and it comprises:
1, has stronger absorbent function, the effect and the effect of weight reducing of prevention intestines problem arranged;
2, have the function of adsorb organic compound and the effect of angiocardiopathy preventing;
3, cation there are combination and function of exchange, can reduce blood fat;
4, can change the physical characteristic that microbiologic population forms in the intestinal tract.Though can not being decomposed by the human consumption enzyme, dietary fiber absorbs, it but is a kind of nutrient that maintains health, can not be replaced by other materials, some countries of west are used for food industry with dietary fiber as a kind of functional food ingredient successively, and some developed countries are recently with its main food as diseases such as prevention colorectal cancer, coronary heart disease, diabetes.The kind skin of cereal, as maize peel, soybean skin wheat bran etc. is to produce the dietary fiber primary raw material, the method of producing dietary fiber both at home and abroad mainly is the mechanical-physical super-fine processing, its production technology is simple relatively, and most of composition of product is an insoluble diedairy fiber, has only few part soluble dietary fiber, by research to the correlation between chemical composition, physico-chemical property and its physiological function of dietary fiber, the physiological function of finding insoluble diedairy fiber is relatively poor, and the solubility composition in the dietary fiber can be brought into play physiological function more.
Summary of the invention
The present invention aims to provide a method of producing soluble dietary fiber, and its process is as follows:
With maize peel, skin of beancurd, rice bran, cereal kind skins such as wheat bran are raw material, with cereal kind skin through crushing screening, the water that adds 2~5 times of volumes, hot digestion made it softening in 5~15 minutes, the ratio adding AMS that adds 10~80 enzymes unit alive then in every gram cereal kind skin, and be 5.5~6.5 at pH value, temperature is an insulated and stirred 1~5 hour under 40~80 ℃ the condition, remove contained starch, the ratio adding protease that adds 10~15 enzymes unit alive then in every gram cereal kind skin, at pH value is 6~8, temperature is an insulated and stirred 1~5 hour under 40~55 ℃ the condition, remove its contained protein, obtained removing the cereal kind skin of starch and albumen, at pH value is 4.6~5.0, temperature is under 45~55 ℃ the condition, the ratio that adds 10~50 enzymes unit alive in every gram cereal kind skin adds zytase and the cellulase that mould produced, stirred enzymolysis through 1~3 hour, with cellulose in the cereal kind skin and xylan hydrolysis is solvable fluidity cellulose and soluble xylan, obtain soluble dietary fiber liquid, concentrate through ultrafiltration and vacuum, obtain high concentration liquid shape dietary fiber, or make the dietary fiber powder through vacuum drying.
The present invention passes through biotechnology, chemical constitution and relative amount that the dietary fiber middle part is grouped into polymer have been changed, fiber molecule and length half fiber molecule have been shortened, increased the ratio of solubility composition in the dietary fiber, improved the physiological function of dietary fiber to human body, test shows, the water-soluble components that the dietary fiber of producing through this method produces is much better than natural dietary fiber in the effect aspect reduction blood lipid level, serum total cholesterol and the low-density lipoprotein cholesterol level.The enzyme treatment technique mild condition that this method provides is easy to control, and cost is lower.
The specific embodiment
With 1000 gram maize peel crushing screenings, the water that adds 2 times of volumes, made it softening in 15 minutes through hot digestion, the ratio adding AMS that adds 35 enzymes unit alive then in every gram maize peel, and be 6 at pH value, temperature is an insulated and stirred 2 hours under 70 ℃ the condition, remove contained starch, the ratio adding protease that adds 30 enzymes unit alive then in every gram maize peel, at pH value is 7.5, temperature is an insulated and stirred 2 hours under 45 ℃ the condition, remove its contained protein, obtain the maize peel that about 550 grams have removed starch and albumen, at pH value is 4.8, temperature was under 50 ℃ the condition, adds 20 enzymes zytase and the cellulase that the ratio of unit adds mould respectively and produced of living in every gram maize peel, stirred enzymolysis through 2 hours, obtaining molecular weight is 110,000 soluble cellulose and molecular weight are 28,000 soluble xylan, both soluble dietary fiber liquid, through ultrafiltration and 60 ℃ of vacuum drying, make soluble dietary fiber powder 105 grams.
Claims (1)
1, produce the method for soluble dietary fiber, with maize peel, skin of beancurd, rice bran, cereal kind skins such as wheat bran are raw material, it is characterized in that cereal kind skin through crushing screening, the water that adds 2~5 times of volumes, made it softening in 5~15 minutes through hot digestion, the ratio adding AMS that adds 10~80 enzymes unit alive then in every gram cereal kind skin, and be 5.5~6.5 at pH value, temperature is an insulated and stirred 1~5 hour under 40~80 ℃ the condition, remove contained starch, the ratio adding protease that adds 10~15 enzymes unit alive then in every gram cereal kind skin, at pH value is 6~8, temperature is an insulated and stirred 1~5 hour under 40~55 ℃ the condition, remove its contained protein, obtained removing the cereal kind skin of starch and albumen, at pH value is 4.6~5.0, temperature is under 45~55 ℃ the condition, the ratio that adds 10~50 enzymes unit alive in every gram cereal kind skin adds zytase and the cellulase that mould produced, stirred enzymolysis through 1~3 hour, with cellulose in the cereal kind skin and xylan hydrolysis is soluble cellulose and soluble xylan, obtain soluble dietary fiber liquid, concentrate through ultrafiltration and vacuum, obtain high concentration liquid shape dietary fiber, or make the dietary fiber powder through vacuum drying.
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CNA021330123A CN1483349A (en) | 2002-09-22 | 2002-09-22 | Method for preparing soluble dietary fibre |
Applications Claiming Priority (1)
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CNA021330123A CN1483349A (en) | 2002-09-22 | 2002-09-22 | Method for preparing soluble dietary fibre |
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Cited By (45)
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CN100353867C (en) * | 2006-03-21 | 2007-12-12 | 上海天之冠可再生能源有限公司 | Extraction of multifunctional diet fibre from wheat bran |
WO2008083513A1 (en) * | 2006-12-13 | 2008-07-17 | Beijing Gingko-Group Biological Technology Co., Ltd. | Black soybean hull extract and process for preparation and use thereof |
CN100429998C (en) * | 2005-07-20 | 2008-11-05 | 武汉工业学院 | Method for preparing wheat bran dietary fiber powder by multi-enzyme step-by-step method |
CN100434443C (en) * | 2006-05-26 | 2008-11-19 | 上海师范大学 | A kind of preparation method of rice bran hemicellulose B |
CN101889599A (en) * | 2010-07-20 | 2010-11-24 | 上海应用技术学院 | A kind of cake containing buckwheat bran dietary fiber and its preparation method |
CN100998381B (en) * | 2006-12-12 | 2010-12-01 | 南昌大学 | Production method of high-purity nano grain dietary fiber |
CN101940327A (en) * | 2010-09-03 | 2011-01-12 | 河南工业大学 | Preparation method of fat substitute using corn peel dietary fiber as matrix |
CN101946891A (en) * | 2010-09-03 | 2011-01-19 | 河南工业大学 | Method for preparing water-soluble dietary fiber of corn peels |
CN101946892A (en) * | 2010-09-06 | 2011-01-19 | 河南工业大学 | Method for preparing corn seed coat dietary fiber |
CN102018183A (en) * | 2010-12-10 | 2011-04-20 | 广西大学 | Method for preparing dietary fiber via mechanical activation and enzymolysis by taking bean dregs as raw material |
CN101416694B (en) * | 2008-11-17 | 2011-06-22 | 江南大学 | A kind of preparation method of wheat bran fat substitute |
CN101156679B (en) * | 2006-10-02 | 2011-07-20 | 何建国 | Rice bran nutrition stock solution and its preparation method |
CN101434980B (en) * | 2008-12-08 | 2011-08-10 | 广东省农业科学院农业生物技术研究所 | Preparation of rice bran short peptide |
CN102154400A (en) * | 2010-12-10 | 2011-08-17 | 广西大学 | Method for preparing dietary fiber from bean dregs serving as raw material by combining steam explosion and enzymolysis |
CN102172271A (en) * | 2011-04-01 | 2011-09-07 | 东北农业大学 | Method for preparing soluble dietary fibers from high-humidity extruded rice bran slag |
CN102178151A (en) * | 2011-04-22 | 2011-09-14 | 淮南市楚丰工贸有限公司 | Method for co-producing rice bran dietary fiber and rice bran protein by utilizing defatted rice bran |
CN102187984A (en) * | 2011-06-27 | 2011-09-21 | 成都大学 | Preparation method for soluble dietary fiber with apple pomace as raw material |
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CN103478400A (en) * | 2013-09-11 | 2014-01-01 | 无锡群硕谷唐生物科技有限公司 | High-purity rice bran protein and rice bran fibers and preparation method thereof |
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CN102308953B (en) * | 2011-09-20 | 2013-06-12 | 华南理工大学 | Preparation method of water-insoluble corn dietary fiber with high oil-holding capacity |
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