CN106889622A - A kind of preparation method of big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber - Google Patents
A kind of preparation method of big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber Download PDFInfo
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- CN106889622A CN106889622A CN201510952772.XA CN201510952772A CN106889622A CN 106889622 A CN106889622 A CN 106889622A CN 201510952772 A CN201510952772 A CN 201510952772A CN 106889622 A CN106889622 A CN 106889622A
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Abstract
The invention discloses a kind of preparation method of big wheat seeds coat dietary fiber, comprise the following steps：By big wheat seeds skin by being centrifugally separating to obtain supernatant a and filter residue after wet-milling, dry grinding, alkali leaching albumen, after filter residue is extracted using homogenate extraction method, supernatant b is centrifugally separating to obtain, it is concentrated under reduced pressure that concentrate is big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber crude product after supernatant a and supernatant b is merged；After concentrate is pre-processed through protease and AMS again, then through three hydrolysis of cellulase, hydrolyzate is concentrated, absolute ethyl alcohol is precipitated, is centrifuged, is dried to obtain water-soluble dietary fiber.In the product for preparing in the process of the present invention, water-soluble dietary fiber accounts for more than 95%, in may be directly applied to the food such as beverage and dairy products.
Description
Technical field
The present invention in food field of deep, and in particular to be a kind of preparation method of big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber.
Background technology
Dietary fiber refers to the summation of edible plants cell, polysaccharide, lignin and the related substances that can not be absorbed by human endogenous' enzymic digestion, as the dietary fiber of the seventh-largest nutrient, not only there is nutritive value higher, moreover it is possible to prevent many diseases, it is essential to body metabolism.It is water-soluble dietary fiber and insoluble diedairy fiber that dietary fiber is divided into two classes.Water-soluble dietary fiber can be combined to be removed internal cholic acid, substantially lowers serum cholesterol, reduces the function of postprandial blood glucose levels, and expands the range of application in food service industry, and market requirement increases year by year.Therefore, dietary fiber receives the expert from different field, including physician, nutritionist, meals man, Food Science man, biochemist, and the extensive attention of alimentary codex and the relevant science decision person of nutrition education, and it is classified as " the seventh-largest nutrient " after carbohydrate, protein, fat, water, minerals and vitamins.Western developed country just sets about carrying out research and development to dietary fiber early in the 70's of twentieth century.And China's tradition meals will not lack dietary fiber based on plant food, thus to dietary fiber research and development using inadequate.It can be said that research and development of the China to dietary fiber at present are still in the starting stage, the processing enterprise of scale is not formed.But, with the continuous improvement of people's living standard, eating habit there occurs great changes, and the situation that cellulose is lacked in meals is on the rise, and causes the rich man's diseases such as obesity, hypertension, coronary heart disease, diabetes, colon cancer more and more universal.Therefore, actively develop dietary fiber research and there is extremely far-reaching realistic meaning using to improving our people's general level of the health.
Barley is one of most important Three major grain crops in the world today, and cultivated area and total output are only second to paddy rice and wheat, but its yield per unit area occupies first, and barley has turned into grain, feed and raw material of industry dual-purpose crop.China is second-biggest-in-the-world barley producing country, and current 80% barley is directly listed or directly consumed through preliminary working with raw grain state, and barley deep processing industry is very undeveloped.
Processing of the China to barley focuses primarily upon the production of feed and starch.But byproduct after being processed for barley --- the research that big wheat seeds skin is recycled is also little, and at a fairly low level, many enterprises are only used as feed or discarded, cause the very big wasting of resources.Compared with other cereal crusts, not only dietary fiber content is high for big wheat seeds skin, and its dry grinding gained crust, compared with wheat bran, rice bran, phytic acid content is low, and the adsorptivity to mineral elements such as calcium zinc is small, and the nutrient absorption influence on human body is smaller.
The method for preparing water-soluble dietary fiber at present mainly has：Extraction method, enzyme process, chemical method, enzyme process combination chemical method, superelevation platen press etc..Reduced because general chemical reagent can make the physiologically active of big wheat seeds coat dietary fiber, do not advocate and chemical method is used to big wheat seeds skin, the method alternately hydrolyzed using bronsted lowry acids and bases bronsted lowry solution more than traditional dietary fiber preparation technology removes desizing and protein, but the local flavor and color and luster of hydrolysis prods are poor, product yield and quality are not easily controlled, and working properties are also had a certain impact.Super-high-pressure homogenization method is during homogenization, using a series of effects such as the high-speed impact during super-high-pressure homogenization, high speed shear, shock wave oscillation, hole blast, expanded, it is broken the glycosidic bond of the insoluble dietary fiber such as macromolecular such as cellulose, hemicellulose, lignin of larger molecular weight, part insoluble dietary fiber (IDF) is converted into the soluble dietary fiber of Non-digestible.
The content of the invention
The present invention is in view of the shortcomings of the prior art, there is provided a kind of preparation method of big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber, and in the sample in this way preparing, water-soluble dietary fiber accounts for more than 95%, in may be directly applied to beverage and dairy products.
To reach above-mentioned purpose, the present invention uses following steps：
(1) pretreatment of big wheat seeds skin：Big wheat seeds skin is carried out into wet-milling using ball mill, is dried after wet-milling, dry grinding crushing is carried out using micronizer after drying, obtain big wheat seeds skin Ultramicro-powder；
(2) big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber crude product is extracted using homogenate extraction method：The big wheat seeds skin Ultramicro-powder that step (1) is obtained is added to carries out alkali leaching albumen in aqueous slkali, be then centrifuged for isolated supernatant a and filter residue；Filter residue is taken, deionized water is added, is extracted using homogenate extraction method, extraction terminates rear centrifugation and obtains supernatant b, be concentrated under reduced pressure after supernatant a and supernatant b is merged, obtain concentrate；
(3) add the mixed enzyme solution of protease and AMS, water bath with thermostatic control oscillation treatment certain hour, suction filtration to retain filtrate 1 in concentrate, obtain filter residue 3, the distillation water washing of filter residue 3, suction filtration retains filtrate 2, obtains filter residue 4；
(4) filter residue 4 is divided into 2.5 parts, take a filter residue 4 and add cellulase solution, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration, retain filtrate 3, filter residue 5 is obtained, a filter residue 4 is added in filter residue 5, add the cushioning liquid of pH values identical with cellulase solution, water bath with thermostatic control oscillation treatment certain hour, suction filtration, retains filtrate 4, obtains filter residue 6；
(5) moiety filter residue 4 is added in filter residue 6, the above-mentioned cellulase solution of a half strength is added, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration retains filtrate 5；
(6) filtrate 1- filtrates 5 are merged together, after the enzyme 10min that gone out in boiling water bath, rotary evaporation in vacuo concentration, concentrate 1 is obtained, 4 times of absolute ethyl alcohols are added in concentrate 1, stood overnight, centrifugation, retains precipitation 1, obtains supernatant 1, supernatant 1 is concentrated through rotary evaporation in vacuo, concentrate 2 is obtained, 4 times of absolute ethyl alcohols are added in concentrate 2, stood overnight, centrifugation, retains precipitation 2；
(7) precipitation 1 and precipitation 2 are dissolved in boiling water after merging, centrifugation, obtain supernatant 2, supernatant 2 is concentrated through rotary evaporation in vacuo, obtains concentrate 3, and 4 times of absolute ethyl alcohols are added in concentrate 3, stand overnight, centrifugation, is precipitated 3, and precipitation 3 obtains big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber product through drying, crushing；
The ball mill of the step (1) is planetary gear ball mill, and the process of the wet-milling is：Big wheat seeds skin is placed in planetary gear ball mill, 300~500r/min of rotating speed, 5~15h of Ball-milling Time.
The micronizer of the step (1) is air-stream type ultrafine pulverizer, and the process that the dry grinding is crushed is：Big wheat seeds skin after by drying is placed in air-stream type ultrafine pulverizer and is crushed, and the operating pressure of the air-stream type ultrafine pulverizer is 0.5~0.8MPa, systematic air flow is 3m3/min, and rotating speed is 2000~3000r/min, and charging rate is 10kg/h.
OH- ion concentrations are 0.15mol/L-0.4mol/L in the aqueous slkali of the step (2), and the big wheat seeds skin Ultramicro-powder is 1 with the mass ratio of aqueous slkali:10-1:30, the alkali leaching albumen temperature is 35-55 DEG C, and the time is 0.5-2.0h.
In the homogenate extraction method of the step (2), the deionized water of addition is 10~30 with the mass ratio of filter residue：1, extraction voltage is 20~80V, and the time of extraction is 2~10min.
The protease of the step (3) is any one of papain, neutral proteinase and alkali protease or several.
The weight/mass percentage composition of protease is 0.2%~1.0% in the mixed enzyme solution of the step (3), the weight/mass percentage composition of AMS is 0.2%~1.0%, the pH values 6.0~8.0 of mixed enzyme solution, mixed enzyme solution process time is 1.0~3.0h, and treatment temperature is 40~60 DEG C.
The concentration of the cellulase solution of the step (4) is 0.05~2.0mg/ml, and pH values are 4.0~6.0, and treatment temperature is 35~60 DEG C, and process time is 0.5~8.0h.
Water bath with thermostatic control oscillation treatment certain hour in the step (2), (3) and (4) refers to that to process the temperature of big wheat seeds skin powder be 40~60 DEG C, and process time is 20~60min.
The step（7）The drying means of precipitation 3 be spray drying, freeze-drying or vacuum drying.
Suction filtration described in the above method is operated using suction filtration method well known in the art.
Specific embodiment
Embodiment 1
(1) pretreatment of big wheat seeds skin：Big wheat seeds skin is carried out into wet-milling using ball mill, is dried after wet-milling, dry grinding crushing is carried out using micronizer after drying, obtain big wheat seeds skin Ultramicro-powder；
(2) big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber crude product is extracted using homogenate extraction method：The big wheat seeds skin Ultramicro-powder that step (1) is obtained is added to carries out alkali leaching albumen in aqueous slkali, be then centrifuged for isolated supernatant a and filter residue；Filter residue is taken, deionized water is added, is extracted using homogenate extraction method, extraction terminates rear centrifugation and obtains supernatant b, be concentrated under reduced pressure after supernatant a and supernatant b is merged, obtain concentrate；
(3) mixed enzyme solution of protease neutral proteinase, alkali protease and AMS is added in concentrate, the weight/mass percentage composition of protease is 0.8% in mixed enzyme solution, and the weight/mass percentage composition of AMS is 0.8%, the pH values 7.5 of mixed enzyme solution, 55 DEG C of water bath with thermostatic control oscillation treatment 2.5h of mixed enzyme solution, suction filtration, retains filtrate 1, obtains filter residue 3, the distillation water washing of filter residue 3, suction filtration, retains filtrate 2, obtains filter residue 4；
(4) filter residue 4 is divided into 2.5 parts, and it is 1.5mg/ml to take a filter residue 4 and add concentration, and pH values are 5.5 cellulase solution, 55 DEG C of water bath with thermostatic control oscillation treatment 6.0h, suction filtration, retains filtrate 3, obtains filter residue 5, a filter residue 4 is added in filter residue 5, add the cushioning liquid of pH values identical with cellulase solution, 55 DEG C of water bath with thermostatic control oscillation treatment 6.0h, suction filtration, retain filtrate 4, obtain filter residue 6；
(5) moiety filter residue 4 is added in filter residue 6, the cellulase solution of a half strength is added, temperature is 55 DEG C of water bath with thermostatic control oscillation treatment 6.0h, and suction filtration retains filtrate 5；
(6) filtrate 1- filtrates 5 are merged together, after the enzyme 10min that gone out in boiling water bath, rotary evaporation in vacuo concentration, concentrate 1 is obtained, 4 times of volume absolute ethyl alcohols of concentrate 1 are added in concentrate 1, stood overnight, centrifugation, retains precipitation 1, obtains supernatant 1, supernatant 1 is concentrated through rotary evaporation in vacuo, concentrate 2 is obtained, 4 times of volume absolute ethyl alcohols of concentrate 2 are added in concentrate 2, stood overnight, centrifugation, retains precipitation 2；
(7) precipitation 1 and precipitation 2 are dissolved in boiling water after merging, centrifugation, obtain supernatant 2, supernatant 2 is concentrated through rotary evaporation in vacuo, obtains concentrate 3, and 4 times of volume absolute ethyl alcohols of concentrate 3 are added in concentrate 3, stand overnight, centrifugation, is precipitated 3, the big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber of the vacuum dried acquisition more than 95% of precipitation 3.
Embodiment 2
(1) pretreatment of big wheat seeds skin：Big wheat seeds skin is carried out into wet-milling using ball mill, is dried after wet-milling, dry grinding crushing is carried out using micronizer after drying, obtain big wheat seeds skin Ultramicro-powder；
(2) big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber crude product is extracted using homogenate extraction method：The big wheat seeds skin Ultramicro-powder that step (1) is obtained is added to carries out alkali leaching albumen in aqueous slkali, be then centrifuged for isolated supernatant a and filter residue；Filter residue is taken, deionized water is added, is extracted using homogenate extraction method, extraction terminates rear centrifugation and obtains supernatant b, be concentrated under reduced pressure after supernatant a and supernatant b is merged, obtain concentrate；
(3) add protease papain enzyme, neutral proteinase and alkali protease in concentrate, and AMS mixed enzyme solution, the weight/mass percentage composition of protease is 0.9% in mixed enzyme solution, the weight/mass percentage composition of AMS is 0.3%, the pH values 6.2 of mixed enzyme solution, 42 DEG C of water bath with thermostatic control oscillation treatment 1.8h of mixed enzyme solution, suction filtration, retain filtrate 1, obtain filter residue 3, the distillation water washing of filter residue 3, suction filtration, retain filtrate 2, obtain filter residue 4；
(4) filter residue 4 is divided into 2.5 parts, and it is 1.8mg/ml to take a filter residue 4 and add concentration, and pH values are 4.5 cellulase solution, 58 DEG C of water bath with thermostatic control oscillation treatment 1.0h, suction filtration, retains filtrate 3, obtains filter residue 5, a filter residue 4 is added in filter residue 5, add the cushioning liquid of pH values identical with cellulase solution, 37 DEG C of water bath with thermostatic control oscillation treatment 7.0h, suction filtration, retain filtrate 4, obtain filter residue 6；
(5) moiety filter residue 4 is added in filter residue 6, the cellulase solution of a half strength is added, temperature is 50 DEG C of water bath with thermostatic control oscillation treatment 3.0h, and suction filtration retains filtrate 5；
(6) filtrate 1- filtrates 5 are merged together, after the enzyme 10min that gone out in boiling water bath, rotary evaporation in vacuo concentration, concentrate 1 is obtained, 4 times of volume absolute ethyl alcohols of concentrate 1 are added in concentrate 1, stood overnight, centrifugation, retains precipitation 1, obtains supernatant 1, supernatant 1 is concentrated through rotary evaporation in vacuo, concentrate 2 is obtained, 4 times of volume absolute ethyl alcohols of concentrate 2 are added in concentrate 2, stood overnight, centrifugation, retains precipitation 2；
(7) precipitation 1 and precipitation 2 are dissolved in boiling water after merging, centrifugation, obtain supernatant 2, supernatant 2 is concentrated through rotary evaporation in vacuo, obtains concentrate 3, and 4 times of volume absolute ethyl alcohols of concentrate 3 are added in concentrate 3, stand overnight, centrifugation, is precipitated 3, precipitation 3 is freeze-dried obtain more than 95% big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber.
Embodiment 3
(1) pretreatment of big wheat seeds skin：Big wheat seeds skin is carried out into wet-milling using ball mill, is dried after wet-milling, dry grinding crushing is carried out using micronizer after drying, obtain big wheat seeds skin Ultramicro-powder；
(2) big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber crude product is extracted using homogenate extraction method：The big wheat seeds skin Ultramicro-powder that step (1) is obtained is added to carries out alkali leaching albumen in aqueous slkali, be then centrifuged for isolated supernatant a and filter residue；Filter residue is taken, deionized water is added, is extracted using homogenate extraction method, extraction terminates rear centrifugation and obtains supernatant b, be concentrated under reduced pressure after supernatant a and supernatant b is merged, obtain concentrate；
(3) mixed enzyme solution of papain and AMS is added in concentrate, the weight/mass percentage composition of protease is 0.2% in mixed enzyme solution, and the weight/mass percentage composition of AMS is 0.2%, the pH values 6.0 of mixed enzyme solution, 40 DEG C of water bath with thermostatic control oscillation treatment 1.0h of mixed enzyme solution, suction filtration, retains filtrate 1, obtains filter residue 3, the distillation water washing of filter residue 3, suction filtration, retains filtrate 2, obtains filter residue 4；
(4) filter residue 4 is divided into 2.5 parts, and it is 0.05mg/ml to take a filter residue 4 and add concentration, and pH values are 4.0 cellulase solution, 35 DEG C of water bath with thermostatic control oscillation treatment 0.5h, suction filtration, retains filtrate 3, obtains filter residue 5, a filter residue 4 is added in filter residue 5, add the cushioning liquid of pH values identical with cellulase solution, 35 DEG C of water bath with thermostatic control oscillation treatment 0.5h, suction filtration, retain filtrate 4, obtain filter residue 6；
(5) moiety filter residue 4 is added in filter residue 6, the cellulase solution of a half strength is added, temperature is 35 DEG C of water bath with thermostatic control oscillation treatment 0.5h, and suction filtration retains filtrate 5；
(6) filtrate 1- filtrates 5 are merged together, after the enzyme 10min that gone out in boiling water bath, rotary evaporation in vacuo concentration, concentrate 1 is obtained, 4 times of volume absolute ethyl alcohols of concentrate 1 are added in concentrate 1, stood overnight, centrifugation, retains precipitation 1, obtains supernatant 1, supernatant 1 is concentrated through rotary evaporation in vacuo, concentrate 2 is obtained, 4 times of volume absolute ethyl alcohols of concentrate 2 are added in concentrate 2, stood overnight, centrifugation, retains precipitation 2；
(7) precipitation 1 and precipitation 2 are dissolved in boiling water after merging, centrifugation, obtain supernatant 2, supernatant 2 is concentrated through rotary evaporation in vacuo, obtains concentrate 3, and 4 times of volume absolute ethyl alcohols of concentrate 3 are added in concentrate 3, stand overnight, centrifugation, is precipitated 3, the big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber of the spray-dried acquisition more than 95% of precipitation 3.
Claims (1)
1. a kind of preparation method of big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber, it is characterised in that comprise the following steps：
(1) pretreatment of big wheat seeds skin：Big wheat seeds skin is carried out into wet-milling using ball mill, is dried after wet-milling, dry grinding crushing is carried out using micronizer after drying, obtain big wheat seeds skin Ultramicro-powder；
(2) big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber crude product is extracted using homogenate extraction method：The big wheat seeds skin Ultramicro-powder that step (1) is obtained is added to carries out alkali leaching albumen in aqueous slkali, be then centrifuged for isolated supernatant a and filter residue；Filter residue is taken, deionized water is added, is extracted using homogenate extraction method, extraction terminates rear centrifugation and obtains supernatant b, be concentrated under reduced pressure after supernatant a and supernatant b is merged, obtain concentrate；
(3) add the mixed enzyme solution of protease and AMS, water bath with thermostatic control oscillation treatment certain hour, suction filtration to retain filtrate 1 in concentrate, obtain filter residue 3, the distillation water washing of filter residue 3, suction filtration retains filtrate 2, obtains filter residue 4；
(4) filter residue 4 is divided into 2.5 parts, take a filter residue 4 and add cellulase solution, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration, retain filtrate 3, filter residue 5 is obtained, a filter residue 4 is added in filter residue 5, add the cushioning liquid of pH values identical with cellulase solution, water bath with thermostatic control oscillation treatment certain hour, suction filtration, retains filtrate 4, obtains filter residue 6；
(5) moiety filter residue 4 is added in filter residue 6, the above-mentioned cellulase solution of a half strength is added, water bath with thermostatic control vibration enzymolysis certain hour, suction filtration retains filtrate 5；
(6) filtrate 1- filtrates 5 are merged together, after the enzyme 10min that gone out in boiling water bath, rotary evaporation in vacuo concentration, concentrate 1 is obtained, 4 times of absolute ethyl alcohols are added in concentrate 1, stood overnight, centrifugation, retains precipitation 1, obtains supernatant 1, supernatant 1 is concentrated through rotary evaporation in vacuo, concentrate 2 is obtained, 4 times of absolute ethyl alcohols are added in concentrate 2, stood overnight, centrifugation, retains precipitation 2；
(7) precipitation 1 and precipitation 2 are dissolved in boiling water after merging, centrifugation, obtain supernatant 2, supernatant 2 is concentrated through rotary evaporation in vacuo, obtains concentrate 3, and 4 times of absolute ethyl alcohols are added in concentrate 3, stand overnight, centrifugation, is precipitated 3, and precipitation 3 obtains big wheat seeds severe edema due to hypofunction of the spleen soluble dietary fiber product through drying, crushing.
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| CN108866122A (en) * | 2018-07-02 | 2018-11-23 | 青海华实科技投资管理有限公司 | A kind of method of water-soluble dietary fiber in extraction highland barley bran |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108866122A (en) * | 2018-07-02 | 2018-11-23 | 青海华实科技投资管理有限公司 | A kind of method of water-soluble dietary fiber in extraction highland barley bran |
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Application publication date: 20170627 |