CN102172271B - Method for preparing soluble dietary fibers from high-humidity extruded rice bran slag - Google Patents
Method for preparing soluble dietary fibers from high-humidity extruded rice bran slag Download PDFInfo
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- CN102172271B CN102172271B CN2011100814608A CN201110081460A CN102172271B CN 102172271 B CN102172271 B CN 102172271B CN 2011100814608 A CN2011100814608 A CN 2011100814608A CN 201110081460 A CN201110081460 A CN 201110081460A CN 102172271 B CN102172271 B CN 102172271B
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- rice bran
- soluble dietary
- bran slag
- slag
- high humidity
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Abstract
The invention provides a method for preparing soluble dietary fibers from high-humidity extruded rice bran slag. In the method, the soluble dietary fiber product of the rice bran can be obtained by carrying out high-humidity extrusion on the rice bran slag, hydrolyzing the high-humidity extruded rice bran slag by using cellulase and treating the rice bran slag by using neutral protease or absolute ethyl alcohol. At present, a plurality of methods, which mainly include an acid process, an alkaline process, an enzymatic process, a fermentation process, a mechanical process, a combining process and the like, are available for preparing the soluble dietary fibers from the rice bran; and the raw materials of the methods are all rice bran slag which are not extruded under high humidity, wherein compared with other processes, the enzymatic process has the advantage of slightly high yield of the soluble dietary fibers; however, the yield is low with respect to the proportion in terms of the total content of the soluble dietary fibers. Because partial insoluble dietary fibers in the rice bran slag can be converted to the soluble dietary fibers by using the method provided by the invention, the method has the advantages of capabilities of increasing content and yield of the soluble dietary fibers and promoting waste recycling, low cost, no addition of acid, alkaline and other chemical ingredients, safety, health and wide application range.
Description
Technical field:
The present invention relates to the processing of farm products field, be specifically related to a kind of method of from high humidity extruding rice bran slag, producing soluble dietary fiber, be applied to the preparation of soluble dietary fiber.
Background technology:
Dietary fiber is called " the 7th nutrient " by nutrition educational circles, it be a kind of can not be by the carbohydrate of human consumption, dietary fiber is by its water-soluble two types of insoluble diedairy fiber and soluble dietary fiber that are divided into.Soluble dietary fiber has the physiological function stronger than insoluble diedairy fiber in many aspects.Soluble Fiber can slow down digestion rate and rapid drainage cholesterol, helps to regulate function of immune system, promotes the discharge of toxic heavy metal in the body, and thus, the preparation soluble dietary fiber has great importance.
The method for preparing the rice bran soluble dietary fiber is a lot, mainly contains acid system, alkaline process, enzyme process, fermentation method, Mechanical Method and combined techniques etc., and its raw material is the rice bran slag without the high humidity extrusion process.Wherein enzyme process and additive method compare, and the soluble dietary fiber productive rate is slightly high, but not high with regard to its its productive rate of ratio that accounts for total dietary fiber content.
Summary of the invention:
The present invention is a kind of method of from high humidity extruding rice bran slag, producing soluble dietary fiber, earlier the rice bran slag is carried out the high humidity extrusion process, then with Enzymatic Extraction soluble dietary fiber wherein.Part insolubility dietary fiber is transformed to soluble dietary fiber, thereby increase the content of soluble dietary fiber in the rice bran slag, the cellulose-binding enzyme process carries out the extraction of soluble dietary fiber to the rice bran slag again; The content and the productive rate of soluble dietary fiber have been improved; Cost is low, has promoted twice laid, and does not add chemical compositions such as soda acid; Safety and Health has wide range of applications.
The objective of the invention is to realize through following technical scheme.
Main design of the present invention is; Because higher and fat content of dietary fiber content and protein,alcohol-soluble content are low in the rice bran slag; So it is handled through cellulase, neutral proteinase hydrolysis, with the absolute ethyl alcohol deposition, can obtain rice bran soluble dietary fiber product again.
Particularly; The preparation method of soluble dietary fiber provided by the invention comprises: will in the rice bran slag that high humidity pushed, add entry; Be hydrolyzed with cellulase; Products therefrom gone out to be hydrolyzed with neutral proteinase behind the enzyme, with the absolute ethyl alcohol deposition, then can obtain rice bran soluble dietary fiber product again.
Wherein, The residual quantity of fat is lower in the rice bran slag that high humidity pushed; Can not influence the extraction of whole soluble dietary fiber; Therefore need not remove fat wherein to its hydrolysis, and pure protein content wherein is also lower, thereby therefore can directly utilizes neutral proteinase to make its hydrolysis remove protein ingredient wherein with lipase.So this method mainly is with cellulase the rice bran slag that high humidity pushed to be hydrolyzed, thereby some insoluble diedairy fibers that will wherein contain transform to soluble dietary fiber, thereby obtain the higher method for preparing the rice bran soluble dietary fiber of productive rate.
The used cellulase of the present invention can be this area cellulase commonly used, and not special the qualification, for example endoglucanase, cellobiohydrolase and beta-glucosidase etc., and these enzymes can use separately, also can make up use.The hydrolysising condition of cellulase can be that 0.2～1.2% (based on the weight of hydrolysis substance dry), PH 3.5～6.5, temperature are that 35～60 ℃, reaction time are 0.5～3h for: solid-liquid ratio 1: 10～1: 22 (the rice bran slag after the high humidity extruding and the ratio of amount of water), enzyme concentration generally.
Through the rice bran soluble dietary fiber product that said method obtains, its productive rate is 24.14%, apparently higher than the rice bran slag (16.72%) without the high humidity extruding.Therefore range of application is very extensive, can be used for producing rice bran soluble dietary fiber food, for example is used to produce the product of soluble dietary fiber electuary class.
Useful point of the present invention is:
The high humidity pressing method is handled the rice bran slag, and part insolubility dietary fiber is transformed to soluble dietary fiber, thereby increases the content of soluble dietary fiber in the rice bran slag; The plain Enzymatic Extraction of reprocess fibre soluble dietary fiber wherein; Cellulase can make that macromolecular cellulose degradation becomes low molecular polysaccharide, oligosaccharides, monose in the rice bran slag; The insolubility dietary fiber is transformed to solvable dietary fiber, further improve the content of soluble dietary fiber in the rice bran slag.Thereby improve the recovery rate of soluble dietary fiber, improved twice laid efficient, reduced cost.
Step of the present invention is following:
Step 1: rice bran slag; Cross 60 mesh sieves; Adopt SYSLG30-IV twin-screw extrusion experiment machine (hundred promise scientific and technological development Co., Ltds are matched in Jinan) to carry out extrusion process, the high humidity extruding condition is: moisture 60%, 30 ℃ of I district temperature, 60 ℃ of II district temperature, 140 ℃ of III district temperature, 150 ℃ of IV district temperature; Screw speed 200rpm; After the extruding, pulverize, subsequent use; Take by weighing a certain amount of rice bran slag after the high humidity extrusion process in reactor, add entry, selecting material-water ratio (the rice bran slag after the high humidity extruding and the ratio of amount of water) is 1: 10～1: 22; Add quality again and be cellulase solution through high humidity extruding back rice bran slag 0.4%, the pH value be 4 and 40 ℃ of temperature under carry out enzyme digestion reaction, the boiling water bath 10min enzyme that goes out; Drop to room temperature, add the neutral protease enzymolysis 1.5h of quality again, centrifugal filtration for rice bran slag 0.2% after the high humidity extruding; Filtrating concentrates, with 4 times of absolute ethyl alcohol depositions, centrifugal filtration; Obtain soluble dietary fiber, measure the content of SDF, confirm material-water ratio.
Step 2: the addition of selecting enzyme be after the high humidity extrusion process rice bran slag 0.2%～1.2%, the same step 1 of process, the addition of the enzyme of confirming to react;
Step 3: the initial pH of choice reaction is 3.5～6, and the same step 1 of process is confirmed the initial pH that reacts;
Step 4: the choice reaction temperature is 35～60 ℃, and the same step 1 of process is confirmed the reaction optimum temperature;
Step 5: the choice reaction time is respectively 0.5～3h, and the same step 1 of process is confirmed the reaction time;
The present invention is to be raw material with the rice bran slag through high humidity extruding, and key is with Enzymatic Extraction confirming of soluble dietary fiber condition wherein, comprises addition, reaction pH, reaction temperature, the reaction time of solid-liquid ratio, enzyme.According to the optimal processing parameter preparation, its productive rate exceeds 7.42% without the rice bran slag of high humidity extruding.Proved that with high humidity extruding rice bran slag be raw material, wherein the method for soluble dietary fiber is very with practical value with Enzymatic Extraction.
The specific embodiment:
Below further describe the present invention through specific embodiment; But these embodiment only are illustrative; Protection scope of the present invention is not constituted any influence; It will be understood by those skilled in the art that any modification and the replacement made according to spirit of the present invention all fall in protection scope of the present invention.
The specific embodiment one: the step of this embodiment is following:
Step 1: rice bran slag; Cross 60 mesh sieves; Adopt SYSLG30-IV twin-screw extrusion experiment machine (hundred promise scientific and technological development Co., Ltds are matched in Jinan) to carry out extrusion process, the high humidity extruding condition is: moisture 60%, 30 ℃ of I district temperature, 60 ℃ of II district temperature, 140 ℃ of III district temperature, 150 ℃ of IV district temperature; Screw speed 200rpm; After the extruding, pulverize, subsequent use; Take by weighing a certain amount of rice bran slag after the high humidity extrusion process in reactor, add entry, selecting material-water ratio (the rice bran slag after the high humidity extruding and the ratio of amount of water) is 1: 10～1: 22; Add quality again and be cellulase solution through high humidity extruding back rice bran slag 0.4%, the pH value be 4 and 40 ℃ of temperature under carry out enzyme digestion reaction, the boiling water bath 10min enzyme that goes out; Drop to room temperature, add the neutral protease enzymolysis 1.5h of quality again, centrifugal filtration for rice bran slag 0.2% after the high humidity extruding; Filtrating concentrates, with 4 times of absolute ethyl alcohol depositions, centrifugal filtration; Obtain soluble dietary fiber, measure the content of SDF, confirm material-water ratio.
Step 2: the addition of selecting enzyme be after the high humidity extrusion process rice bran slag 0.2%～1.2%, the same step 1 of process, the addition of the enzyme of confirming to react;
Step 3: the initial pH of choice reaction is 3.5～6, and the same step 1 of process is confirmed the initial pH that reacts;
Step 4: the choice reaction temperature is 35～60 ℃, and the same step 1 of process is confirmed the reaction optimum temperature;
Step 5: the choice reaction time is respectively 0.5～3h, and the same step 1 of process is confirmed the reaction time;
The practical implementation method two: this implementation method is in the step 1 material-water ratio that reacts to be controlled at 1: 12～1: 20 with practical implementation method one difference, and other composition is identical with practical implementation method one with step;
Practical implementation method three: this implementation method is that with practical implementation method one difference the material-water ratio that reacts in the step 2 was controlled at 1: 12～1: 17, and other composition is identical with practical implementation method one with step;
Practical implementation method four: this implementation method is that with practical implementation method one difference the addition of the enzyme of choice reaction in the step 3 is 0.4%～1.2% of rice bran slag after the high humidity extrusion process, and other composition is identical with practical implementation method one with step;
Practical implementation method five: the addition of the enzyme that this implementation method and practical implementation method one difference are to react in the step 4 is 0.6%～1% of rice bran slag after the high humidity extrusion process, and other composition is identical with practical implementation method one with step;
Practical implementation method six: this implementation method is that with practical implementation method one difference the initial pH that reacts in the step 5 is 3.5～5.5, and other composition is identical with practical implementation method one with step;
Practical implementation method seven: this implementation method is that with practical implementation method one difference the initial pH that reacts in the step 5 is 4.0～5.0, and other composition is identical with practical implementation method one with step;
Practical implementation method eight: this implementation method is that with practical implementation method one difference the choice reaction temperature is 40～60 ℃ in the step 5, and other composition is identical with practical implementation method one with step;
Practical implementation method nine: this implementation method is that with practical implementation method one difference the choice reaction temperature is 45～55 ℃ in the step 5, and other composition is identical with practical implementation method one with step;
Practical implementation method ten: this implementation method is that with practical implementation method one difference the choice reaction time is respectively 1～2h in the step 5, and other composition is identical with practical implementation method one with step.
Claims (1)
1. a method of from high humidity extruding rice bran slag, producing soluble dietary fiber is carried out the method that soluble dietary fiber prepares under certain reaction condition, and its characterization step is following:
Step 1: rice bran slag; Cross 60 mesh sieves; The SYSLG30-IV twin-screw extrusion experiment machine that adopts Jinan to match hundred promise scientific and technological development Co., Ltds carries out extrusion process, and the high humidity extruding condition is: moisture 60%, 30 ℃ of I district temperature, 60 ℃ of II district temperature, 140 ℃ of III district temperature, 150 ℃ of IV district temperature; Screw speed 200rpm; After the extruding, pulverize, subsequent use; Take by weighing a certain amount of rice bran slag after the high humidity extrusion process in reactor, add entry, selecting material-water ratio is 1: 10～1: 22, and said material-water ratio be rice bran slag and the ratio of amount of water after high humidity pushes; Add quality again and be cellulase solution through high humidity extruding back rice bran slag 0.4%, the pH value be 4 and 40 ℃ of temperature under carry out enzyme digestion reaction, the boiling water bath 10min enzyme that goes out; Drop to room temperature, add the neutral protease enzymolysis 1.5h of quality again, centrifugal filtration for rice bran slag 0.2% after the high humidity extruding; Filtrating concentrates, with 4 times of absolute ethyl alcohol depositions, centrifugal filtration; Obtain soluble dietary fiber, measure the content of SDF, confirm material-water ratio.
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| CN102550934B (en) * | 2012-01-11 | 2014-09-24 | 东方集团粮油食品有限公司 | Full-cream stable rice bran powder |
| ES2665773T3 (en) * | 2012-10-22 | 2018-04-27 | Dsm Ip Assets B.V. | Smooth hydrolysis of rice bran proteins |
| CN105341952B (en) * | 2015-11-26 | 2017-12-12 | 中国农业大学 | A kind of method of soluble dietary fiber in extrusion assistance enzymolysis extraction rice bran |
| CN109170429A (en) * | 2018-09-06 | 2019-01-11 | 山东禹王生态食业有限公司 | A kind of preparation method of modified bean dregs dietary fiber beverage |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483349A (en) * | 2002-09-22 | 2004-03-24 | 于铁梁 | Method for preparing soluble dietary fibre |
| CN101161103A (en) * | 2007-11-23 | 2008-04-16 | 东北农业大学 | Method for preparing bean slags soluble food fibers by zymolysis method |
| CN101785831A (en) * | 2010-03-09 | 2010-07-28 | 中南林业科技大学 | Preparation method of rice bran dietary fiber |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1483349A (en) * | 2002-09-22 | 2004-03-24 | 于铁梁 | Method for preparing soluble dietary fibre |
| CN101161103A (en) * | 2007-11-23 | 2008-04-16 | 东北农业大学 | Method for preparing bean slags soluble food fibers by zymolysis method |
| CN101785831A (en) * | 2010-03-09 | 2010-07-28 | 中南林业科技大学 | Preparation method of rice bran dietary fiber |
Non-Patent Citations (4)
| Title |
|---|
| 孙兰萍等.挤压法制备米糠膳食纤维的研究.《食品工业科技》.2005,(第04期),99-100. |
| 挤压法制备米糠膳食纤维的研究;孙兰萍等;《食品工业科技》;20050425(第04期);99-100 * |
| 用挤压法提高米糠中可溶性膳食纤维含量的研究;许晖;《食品与机械》;19991031(第06期);19-20 * |
| 许晖.用挤压法提高米糠中可溶性膳食纤维含量的研究.《食品与机械》.1999,(第06期),19-20. |
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Inventor after: Yu Dianyu Inventor after: Li Yue Inventor after: Zhang Jianing Inventor after: Li Zhiping Inventor after: Shi Min Inventor after: Chen Xiaohui Inventor after: Jiang Lianzhou Inventor after: Zhang Hongwei Inventor after: Hu Lizhi Inventor after: Wang Yan Inventor after: Luo Shunian Inventor after: Zhu Xiuqing Inventor after: Wang Liqi Inventor after: Wang Xue Inventor before: Yu Dianyu Inventor before: Shi Min Inventor before: Chen Xiaohui Inventor before: Jiang Lianzhou Inventor before: Zhu Xiuqing Inventor before: Wang Liqi Inventor before: Wang Yan Inventor before: Wang Xue Inventor before: Li Yue Inventor before: Zhang Jianing Inventor before: Li Zhiping |
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Free format text: CORRECT: INVENTOR; FROM: YU DIANYU JIANG LIANZHOU ZHU XIUQING WANG LIQI WANG YAN WANG XUE LI YUE ZHANG JIANING LI ZHIPING SHI MIN CHEN XIAOHUI TO: YU DIANYU JIANG LIANZHOU ZHANG HONGWEI HU LIZHI WANG YAN LUO SHUNIAN ZHU XIUQING WANG LIQI WANG XUE LI YUE ZHANG JIANING LI ZHIPING SHI MIN CHEN XIAOHUI |
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