CN104206805A - Preparation for degrading vivotoxin and enhancing anti-stress capability of aquatic animals - Google Patents
Preparation for degrading vivotoxin and enhancing anti-stress capability of aquatic animals Download PDFInfo
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- CN104206805A CN104206805A CN201410348888.8A CN201410348888A CN104206805A CN 104206805 A CN104206805 A CN 104206805A CN 201410348888 A CN201410348888 A CN 201410348888A CN 104206805 A CN104206805 A CN 104206805A
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Abstract
The invention discloses an application of a preparation for degrading vivotoxins and enhancing an anti-stress capability of aquatic animals. The preparation includes 0.1-22% by weight of an aminopolysaccharide active ingredient, wherein a molecular weight of the aminopolysaccharide is 2000-4000000 and a degree of deacetylation of the aminopolysaccharide is more than 50%. A solvent is an acid solution with a concentration being 0.1-30%. In addition, the preparation includes 100-2500 mg/L of vitamin C, 1-100 mg/L of vitamin B1, 1-100 mg/L of vitamin B2 and 1-100 mg/L of vitamin B6. The components, through scientific compatibility, are mixed with various aquatic feeds and account for 0.5-1% of a weight percentage ratio of the feeds. The preparation can enhance the anti-stress capability, immunity and an anti-diseases capability, can degrade residual harmful substances, such as drugs, heavy metals and the like, and can promote growth of the animals. The preparation can be added for a long time during a whole breeding process, is ecological, green and free of pollution, can reduce cost under a modern high-density intensive culture mode, can increase an output value and is an excellent selection for aquatic breeding in future.
Description
Technical field
The present invention relates to a kind of aquatic animal body endotoxin of degrading, improve the preparation of resisting stress power.In particular, a kind of aquatic animal body endotoxin of degrading, the preparation improving resisting stress power and application thereof is related to.
Background technology
At present, developing rapidly of culture fishery makes aquaculture scale constantly expand, cultured output sustainable growth.But along with cultivation intensive degree improves constantly, the stressed condition in aquatic livestock living environment is increasing, disease is also more and more serious, and the disease prevention and cure of aquatic livestock and healthy aquaculture obtain increasing concern.Traditional disease control mainly uses the chemicalses such as antibiotic to suppress or kills germ, but aquatic livestock constantly increases antibiotic drug resistance, and its immunologic function declines, and the residual problem of medicine is day by day serious, affects the safety of food and the health of the mankind.People start the physique attempting improving aquatic livestock by immunologic method, and strengthen their resistances to pathogenic microorganism, therefore the development and application of aquatic livestock immunologic active material becomes focus.
The main component of the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is a kind of native amino polysaccharide extracted from the crustacean of deep-sea, being the edibility animal origin at present in the world uniquely containing free amine group basic group, is the rare positively charged alkaline polysaccharide of occurring in nature.It is biosynthetic natural polymer, and molecular weight is between 2000 ~ 4000000, and deacetylation, up to more than 50%, has excellent bio-compatibility, biodegradable, catabolite safety non-toxic, and has the characteristics such as anti-oxidant, antitoxin, antibacterial.The preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is mixed by glycosaminoglycan and the multivitamin such as vitamin C, vitamin B1, B2, B6, it is the polysaccharide immunologic active material of a kind of green, safety, aquatic animal body of degrading endotoxin, improve aquatic livestock nospecific immunity factor active, effectively evoke the Antiviral Mechanism of body, and easy to use.
Summary of the invention
The object of the invention is to, provide a kind of can at the widely used aquatic animal body endotoxin of can degrading of various aquiculture animal, the preparation improving resisting stress power.
The technical scheme that the present invention solves degraded aquatic animal body endotoxin, raising resisting stress power problem adopts is:
The preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power, following component is comprised: glycosaminoglycan active component 0.1 ~ 22% according to percentage by weight, glycosaminoglycan molecular weight is between 2000 ~ 4000000, deacetylation is up to 50%, solvent is the acid solution of 0.1 ~ 30% concentration, add vitamin C 100 ~ 2500mg/L in addition, Cobastab
11 ~ 100mg/L, Cobastab
21 ~ 100mg/L, Cobastab
61 ~ 100mg/L.
A preparation for aquatic animal body of degrading endotoxin, raising resisting stress power, is mixed in various aquatic feeds, accounts for 0.5 ~ 1% of feed relative percentage.
The formulation application of degraded aquatic animal body endotoxin of the present invention, raising resisting stress power, in various aquatic livestock, has following effect and advantage:
Containing glycosaminoglycan active component 0.1 ~ 22% in this product, glycosaminoglycan molecular weight is between 2000 ~ 4000000, and deacetylation is up to 50%.Glycosaminoglycan is a kind of macromolecular compound of straight chain type, hydrogen bond, covalent bond or coordinate bond is formed by the hydroxyl on its strand, amino and organic matter or metal ion, produce adsorption-flocculation effect, the heavy metals such as effective complexing animal body interior lead, mercury, copper, degraded antibiotic etc remains.Enter after in aquatic animal body, can degrade multiple antibiotic residues in aquatic animal body and toxin accumulation, recover the ecosystem state of aquatic livestock.In addition, glycosaminoglycan active component can improve aquatic livestock phenol oxidase (PO) vigor, bacteriolyze (U
l) immune indexes such as vigor, acid phosphatase (ACP) vigor, superoxide dismutase (SOD) vigor, improve immunity and the premunition of aquatic livestock.
Containing vitamin C 100 ~ 2500mg/L in this product, it is a kind of vitamine supplement generally used in aquaculture process, the functions such as it has promotion collagen synthesis, anti-oxidant, Vitamin C disease, are widely used in aquatic livestock resisting stress, improve the aspect such as immunity, growth promotion.
Containing Cobastab in this product
11 ~ 100mg/L, Cobastab
21 ~ 100mg/L, Cobastab
61 ~ 100mg/L, vitamin B complex vitamin is the essential amino acid needed for aquatic animal to grow, has and regulates metabolism, maintain the health of skin and muscle, promote immune system and nervous system, the function of Promote cell's growth and division, is widely used in the middle of aquaculture.
Because this prescription adopts glycosaminoglycan, vitamin C, Cobastab
1, Cobastab
2, Cobastab
6make Deng cooperation, effective degraded medicament residue, raising aquatic livestock immunity and premunition, improve cultured output, reduce feed coefficient and the incidence of disease, can add for a long time in whole breeding process, ecological, green, pollution-free, reduce costs under being applicable to modern high density culture pattern, improving the output value, is an excellent selection of aquaculture from now on.
Accompanying drawing explanation
This product can improve aquatic livestock phenol oxidase (PO) vigor, bacteriolyze (U
l) immune indexes such as vigor, acid phosphatase (ACP) vigor, superoxide dismutase (SOD) vigor, improve immunity and the premunition of aquatic livestock.
Accompanying drawing 1 is the impact of this product on prawn phenol oxidase (PO) vigor.
Accompanying drawing 2 is that this product is to prawn bacteriolyze (U
l) impact of vigor.
Accompanying drawing 3 is the impact of this product on prawn acid phosphatase (ACP) vigor.
Accompanying drawing 4 is the impact of this product on prawn superoxide dismutase (SOD) vigor.
Detailed description of the invention
Below in conjunction with concrete embodiment, the present invention is described in further detail:
Get glycosaminoglycan, vitamin C, Cobastab
1, Cobastab
2, Cobastab
6, acetic acid is for subsequent use.
Further illustrate the present invention below in conjunction with concrete embodiment, and the present invention is not limited.
Embodiment 1
Be 80000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 50g of 55%, acetic acid 40g join in 910g water, and abundant stirring and dissolving, until completely dissolved, then adds 1g vitamin C, 10mg Cobastab
1, Cobastab
210mg/L, Cobastab
610mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 200kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Embodiment 2
Be 200000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 100g of 65%, acetic acid 100g join in 800g water, and abundant stirring and dissolving, until completely dissolved, then adds 1.5g vitamin C, 15mg Cobastab
1, Cobastab
215mg/L, Cobastab
615mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 180kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Embodiment 3
Be 1000000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 160g of 60%, acetic acid 40g join in 800g water, and abundant stirring and dissolving, until completely dissolved, then adds 1g vitamin C, 8mg Cobastab
1, Cobastab
28mg/L, Cobastab
68mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 160kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Embodiment 4
Be 50000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 150g of 60%, acetic acid 50g join in 800g water, and abundant stirring and dissolving, until completely dissolved, then adds 1g vitamin C, 20mg Cobastab
1, Cobastab
220mg/L, Cobastab
620mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 150kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Embodiment 5
Be 600000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 100g of 60%, acetic acid 30g join in 870g water, and abundant stirring and dissolving, until completely dissolved, then adds 1.2g vitamin C, 30mg Cobastab
1, Cobastab
230mg/L, Cobastab
630mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 110kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Embodiment 6
Be 200000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 160g of 60%, acetic acid 40g join in 800g water, and abundant stirring and dissolving, until completely dissolved, then adds 1.1g vitamin C, 8mg Cobastab
1, Cobastab
28mg/L, Cobastab
68mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 120kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Embodiment 7
Be 2000000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 100g of 60%, acetic acid 80g join in 820g water, and abundant stirring and dissolving, until completely dissolved, then adds 1.2g vitamin C, 18mg Cobastab
1, Cobastab
218mg/L, Cobastab
618mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 130kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Embodiment 8
Be 1500000 by mean molecule quantity, deacetylation is that the glycosaminoglycan 150g of 60%, acetic acid 50g join in 800g water, and abundant stirring and dissolving, until completely dissolved, then adds 1.2g vitamin C, 10mg Cobastab
1, Cobastab
210mg/L, Cobastab
610mg/L, makes a kind of aquatic animal body endotoxin of degrading, improves the preparation of resisting stress power.
Get feed 120kg, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power is sprayed on feed, sprays evenly, after drying, can bait be obtained.
Using method:
By the bait made above, throw something and feed and comprise sea cucumber, prawn, river crab, soft-shelled turtle, eel six kinds of breed varieties, sooner or later respectively once, within every 10 days, throw something and feed continuously 5 days.
Laboratory report:
1, Penglai, shandong Province breading sea cucumber seedlings pond:
Sea cucumbers pond, arranges six seedling ponds, and one as a control group, original bait of throwing something and feeding, the different bait feedings in another 5 above-mentioned examples of selection.
Result: the stomach liquid and the bile fluid that extract each pond seedling, measures phenol oxidase (PO) vigor, the bacteriolyze (U in extract
l) immune indexes such as vigor, acid phosphatase (ACP) vigor, superoxide dismutase (SOD) vigor, throw something and feed and added the ginseng pond of this preparation, its enzyme activity is obviously better than control group, particularly use the ginseng pond of embodiment 1, enzyme activity is the highest, other ginseng pond is also significantly improved the effect of enzymatic activity, and employ the ginseng pond of adding this preparation, seedling state is all obviously better than control group, control group seedling has occurred that in breeding process corn is sent out blunt, dragged the symptom such as just, and growth conditions is poor.
2, Zhanjiang shrimp culture pond
The prawn culturing pool, arranges three ponds, and one as a control group, another two bait feedings using embodiment 1 and embodiment 8 respectively.
Result: the shrimp pond of this preparation of having thrown something and fed, prawn growth conditions is obviously better than control group, and shrimp seedling body colour green grass or young crops is bright, and spring and vigor are all better than control group.Extract the serum of each pond prawn, measure the immune indexes such as phenol oxidase (PO) vigor, bacteriolyze (UL) vigor, acid phosphatase (ACP) vigor, superoxide dismutase (SOD) vigor of prawn serum, throw something and feed and added the shrimp pond of this preparation, its enzyme activity is obviously better than control group, the pond of embodiment 1, the enzymatic activity of shrimp body activity ratio embodiment 8 is high.This preparation is rich in multivitamin, more energy is provided, reduce permeability of cell membrane, make animal be subject to stress time cell membrane change few, the anti-stress ability of prawn can be significantly improved, make prawn stress reaction when various condition change not obvious, ingest, grow all normally, significantly maintain the healthy growth of prawn.And control group shrimp is recuperated and grows the later stage and occurred swimming the pool, raise the nose above water to breathe to steal dead situation, the shrimp body in another two ponds does not occur stealing dead situation in a large number.
3, the Changshu, Jiangsu Province culture of Chinese mitten crab pool
The culture of Chinese mitten crab pool, arranges three pools, and one as a control group, another two bait feedings using embodiment 1 and embodiment 5 respectively.
Result: current culture of Chinese mitten crab pattern has had some to change more a few years ago, and aquaculture model is science more, seldom, topmost measure is regulating and controlling water quality to river crab disease, and vast culture of Chinese mitten crab family is it is of concern that the quality of river crab, quality price determination.This preparation be rich in river crab shelling, growth needed for various trace elements, nutrition is closer to river crab body, supplement the nutrition of balance, help crust synthesizes, supplement unknown duricrust and growth factor, can promote that river crab shells smoothly, quick duricrust, and after each shell, the growth rate improving river crab reaches more than 10%.After adding this preparation, river crab is in the cultivation later stage, and the speed of growth is obviously better than control group, and crab oil and crab cream account for the proportion of health higher than control group, and improve river crab quality significantly, economic benefit obviously increases.
4, flat turtle culture pond is faced in Zhejiang
Breeding soft-shell turtle temperature canopy, arranges three canopies, and one in contrast, another two bait feedings adding embodiment 1 and embodiment 6 respectively.
Result: current breeding soft-shell turtle is all much take warm canopy to cultivate, and cultivation density is larger, soft-shelled turtle disease is relatively more serious, and the soft-shelled turtle grown under the wild or imitative wild state on the comparatively outer pool of soft-shelled turtle quality is mutually far short of what is expected.Through adding the bait feeding of this preparation, soft-shelled turtle body colour is more close to wild body colour, and disease is little, and premunition and immunity obviously strengthen.
5, Shunde eel culture field
Shuande one Ge Man factory, because antibiotic and the toxin remains exceed standard and cannot export, economic loss is larger.Add the bait of this preparation through a period of time of throwing something and feeding, through inspection, antibiotic and the toxin remains are degraded gradually, and content reduces gradually, after reaching commercial specification, meet export standard.
Aquaculture new concept started by the preparation of degraded aquatic animal body endotoxin provided by the invention, raising resisting stress power, it is a kind of new method improving culture benefit, have and improve cultivation object-immunity power and premunition, effects such as medicament residue of degrading, long-term taking can improve the yield and quality of crustacean, be the optimal path of green cultivation, there are wide market prospects.
Although embodiment of the present invention are open as above, but it is not restricted to utilization listed in description and embodiment, it can be applied to various applicable the field of the invention completely, for those skilled in the art, can be easy to realize other amendment, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the embodiment described.
Claims (8)
1. an aquatic animal body endotoxin of degrading, improve the preparation of resisting stress power, it is characterized in that according to percentage by weight: glycosaminoglycan active component 0.1 ~ 22%, glycosaminoglycan molecular weight is between 2000 ~ 4000000, and deacetylation is up to 50%, and solvent is the acid solution of 0.1 ~ 30% concentration.
2. the preparation of a kind of aquatic animal body endotoxin of degrading according to claim 1, raising resisting stress power, characterized by further comprising vitamin C 100 ~ 2500mg/L.
3. the preparation of a kind of aquatic animal body endotoxin of degrading according to claim 1, raising resisting stress power, characterized by further comprising Cobastab
11 ~ 100mg/L.
4. the preparation of a kind of aquatic animal body endotoxin of degrading according to claim 1, raising resisting stress power, characterized by further comprising Cobastab
21 ~ 100mg/L.
5. the preparation of a kind of aquatic animal body endotoxin of degrading according to claim 1, raising resisting stress power, characterized by further comprising Cobastab
61 ~ 100mg/L.
6. the preparation of a kind of aquatic animal body endotoxin of degrading according to claim 1, raising resisting stress power, is characterized in that this acid solution is organic acid.
7. the preparation of a kind of aquatic animal body endotoxin of degrading according to claim 6, raising resisting stress power, is characterized in that this organic acid is acetic acid.
8. above requirement, the preparation of a kind of aquatic animal body endotoxin of degrading, raising resisting stress power, is mixed in various aquatic feeds, accounts for 0.5 ~ 1% of feed relative percentage.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410348888.8A CN104206805A (en) | 2014-07-22 | 2014-07-22 | Preparation for degrading vivotoxin and enhancing anti-stress capability of aquatic animals |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410348888.8A CN104206805A (en) | 2014-07-22 | 2014-07-22 | Preparation for degrading vivotoxin and enhancing anti-stress capability of aquatic animals |
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| CN104206805A true CN104206805A (en) | 2014-12-17 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110973410A (en) * | 2019-12-29 | 2020-04-10 | 海南卓越生物有限公司 | Formula and preparation method of in-vivo and in-vitro antidote for aquatic animals |
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| CN1199050A (en) * | 1997-05-08 | 1998-11-18 | 张偲 | Method for preparation of mother-of-pearl glycosaminoglycan |
| CN1279028A (en) * | 1999-10-08 | 2001-01-10 | 张偲 | Pearl shell additive of feed and its preparing process |
| CN101530168A (en) * | 2009-04-16 | 2009-09-16 | 浙江大学 | Additive of organic zinc feed and preparation method and use thereof |
| CN102406061A (en) * | 2011-11-17 | 2012-04-11 | 南京农业大学 | Composite premixed feed for reducing heavy metal residue in animal products as well as preparation method and application thereof |
| CN102742727A (en) * | 2012-05-22 | 2012-10-24 | 众合发(北京)生物科技发展有限公司 | Glycosaminoglycan and vitamin mixed ecological growth-promoting and immune-modulating agent used in aquaculture |
-
2014
- 2014-07-22 CN CN201410348888.8A patent/CN104206805A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1199050A (en) * | 1997-05-08 | 1998-11-18 | 张偲 | Method for preparation of mother-of-pearl glycosaminoglycan |
| CN1279028A (en) * | 1999-10-08 | 2001-01-10 | 张偲 | Pearl shell additive of feed and its preparing process |
| CN101530168A (en) * | 2009-04-16 | 2009-09-16 | 浙江大学 | Additive of organic zinc feed and preparation method and use thereof |
| CN102406061A (en) * | 2011-11-17 | 2012-04-11 | 南京农业大学 | Composite premixed feed for reducing heavy metal residue in animal products as well as preparation method and application thereof |
| CN102742727A (en) * | 2012-05-22 | 2012-10-24 | 众合发(北京)生物科技发展有限公司 | Glycosaminoglycan and vitamin mixed ecological growth-promoting and immune-modulating agent used in aquaculture |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110973410A (en) * | 2019-12-29 | 2020-04-10 | 海南卓越生物有限公司 | Formula and preparation method of in-vivo and in-vitro antidote for aquatic animals |
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Application publication date: 20141217 |