CN104204231A - 用于传染性皮下及造血器官坏死病毒的实时病毒检测方法和试剂盒 - Google Patents

用于传染性皮下及造血器官坏死病毒的实时病毒检测方法和试剂盒 Download PDF

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Publication number
CN104204231A
CN104204231A CN201380011125.8A CN201380011125A CN104204231A CN 104204231 A CN104204231 A CN 104204231A CN 201380011125 A CN201380011125 A CN 201380011125A CN 104204231 A CN104204231 A CN 104204231A
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Prior art keywords
ihhnv
real
fluorescent
seq
fluorescent probe
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CN201380011125.8A
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English (en)
Chinese (zh)
Inventor
苏巴·A/P·巴哈苏
诺芬娜·雅士明·宾蒂奥斯曼
华朋·楚
徒雷·昆
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Universiti Malaya
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Universiti Malaya
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201380011125.8A 2012-02-27 2013-01-15 用于传染性皮下及造血器官坏死病毒的实时病毒检测方法和试剂盒 Pending CN104204231A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
MYPI2012700037A MY184600A (en) 2012-02-27 2012-02-27 Real-time viral detection method and kit for infectious hypodermal and haematopoietic necrosis virus
MYPI2012700037 2012-02-27
PCT/MY2013/000004 WO2013129906A1 (en) 2012-02-27 2013-01-15 Real-time viral detection method and kit for infectious hypodermaland haematopoietic necrosis virus

Publications (1)

Publication Number Publication Date
CN104204231A true CN104204231A (zh) 2014-12-10

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CN201380011125.8A Pending CN104204231A (zh) 2012-02-27 2013-01-15 用于传染性皮下及造血器官坏死病毒的实时病毒检测方法和试剂盒

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Country Link
CN (1) CN104204231A (https=)
IN (1) IN2014DN07968A (https=)
MY (1) MY184600A (https=)
WO (1) WO2013129906A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108531657A (zh) * 2018-05-09 2018-09-14 鲁东大学 对虾传染性皮下及造血组织坏死病毒的荧光定量pcr检测引物组及检测试剂盒
CN112176111A (zh) * 2020-11-05 2021-01-05 北京市水产技术推广站 一种检测传染性胰脏坏死病毒的引物和探针及试剂盒与检测方法
CN116200539A (zh) * 2022-12-19 2023-06-02 中国海洋大学三亚海洋研究院 一种基于era技术的传染性皮下及造血组织坏死病毒快速检测方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410549A (zh) * 2001-09-21 2003-04-16 中国科学院南海海洋研究所 快速检测对虾传染性皮下及造血组织坏死病毒试剂盒
CN1786711A (zh) * 2005-10-23 2006-06-14 王学海 对虾ihhnv的fq－pcr诊断试剂盒及检测方法
CN1936550A (zh) * 2006-08-29 2007-03-28 中国科学院南海海洋研究所 检测对虾传染性皮下及造血组织坏死病毒的试剂盒及方法
CN101643793A (zh) * 2009-08-27 2010-02-10 中国科学院南海海洋研究所 一种用于检测对虾传染性皮下及造血组织坏死病毒的试剂盒及其检测方法
CN101792817A (zh) * 2009-11-30 2010-08-04 浙江大学 对虾ihhnv的检测方法及所用核酸等温扩增检测试剂盒

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7732142B2 (en) * 2006-08-24 2010-06-08 E.I. Du Pont De Nemours And Company Sequences diagnostic for shrimp pathogens

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1410549A (zh) * 2001-09-21 2003-04-16 中国科学院南海海洋研究所 快速检测对虾传染性皮下及造血组织坏死病毒试剂盒
CN1786711A (zh) * 2005-10-23 2006-06-14 王学海 对虾ihhnv的fq－pcr诊断试剂盒及检测方法
CN1936550A (zh) * 2006-08-29 2007-03-28 中国科学院南海海洋研究所 检测对虾传染性皮下及造血组织坏死病毒的试剂盒及方法
CN101643793A (zh) * 2009-08-27 2010-02-10 中国科学院南海海洋研究所 一种用于检测对虾传染性皮下及造血组织坏死病毒的试剂盒及其检测方法
CN101792817A (zh) * 2009-11-30 2010-08-04 浙江大学 对虾ihhnv的检测方法及所用核酸等温扩增检测试剂盒

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
YUE ZQ 等: "Development of real-time polymerase chain reaction assay with TaqMan probe for the quantitative detection of infectious hypodermal and hematopoietic necrosis virus from shrimp", 《J AOAC INT. 》 *
杨冰 等: "对虾传染性皮下及造血组织坏死病毒(IHHNV)PCR检测方法的建立", 《海洋水产研究》 *
王忠发 等: "实时荧光定量PCR快速检测对虾IHHNV载量方法的建立和应用", 《中国卫生检验杂志》 *

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MY184600A (en) 2021-04-07
WO2013129906A1 (en) 2013-09-06
IN2014DN07968A (https=) 2015-05-01

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Application publication date: 20141210