CN104189803A - Traditional Chinese medicine composition for treating pancreatic necrosis of salmons and trout - Google Patents

Abstract

The invention provides a traditional Chinese medicine composition for treating pancreatic necrosis of salmons and trout. The composition is prepared from the following components by weight: 100-150g of barbated skullcup herb, 100-150g of radix trichosanthis, 100-150g of peonies, 60-80g of baical skullcap root, 60-80g of radix rehmanniae, 60-80g of gentian, 60-80g of akebia stem, 60-80g of medicinal evodia fruit, 60-80g of fennel, 60-80g of szechwan chinaberry fruit, 60-80g of immature tangerine peel, 60-80g of achyranthes aspera, 60-80g of plantain seed, 40-60g of radix codonopsis, 40-60g of radix astragali, 40-60g of isatis root, 40-60g of figwort root, 40-60g of orange fruit, 40-60g of medicated leaven, 40-60g of radix bupleuri, 40-60g of gingers, 20-30g of liquorice, 5-20mg of PVP-1 and 5-20g of phytohemagglutinin. The composition is simple in proportioning, is convenient to use, has inhibiting effects on the IPNV (infectious pancreatic necrosis virus), is specially used for pancreatic necrosis of salmons and trout, has the functions of nourishing the liver and kidneys, strengthening the spleen and sterilizing, and has good treatment effects.

Description

The pancreatic necrosis disease Chinese medicine composition for the treatment of salmon, Squaliobarbus ourriculus

 

technical field:

The present invention relates to technical field of pharmaceuticals, relate in particular to a kind of pancreatic necrosis disease Chinese medicine composition for the treatment of salmon, Squaliobarbus ourriculus.

 

background technology:

Before 3 monthly ages of salmon, Squaliobarbus ourriculus, morrhua, sole etc., easily infect this disease, it can enter in water with carrier's excrement, urine, gonad secretions, carries out horizontal transmission, can directly propagate through ovum, also can through the cheek and mouthful and infect, hazardness is large, infectiousness is strong.Cause of disease: infectivity pancreatic necrosis virus (IPNV), virion is positive 20 bodies, without cyst membrane, has 92 capsomeres, and diameter 50-75nm is surrounded by the 1 AMPLIGEN gene being comprised of 2 fragments in capsid; It is RNA viruses minimum in known fishes virus.Symptom: the imbalance of sick fish swimming, often do vertical revolution swimming, just sink under water soon, have a rest and repeat again in a moment above swimming, until dead, generally start revolution and swim to death 1-2 hour only.The sick fish that loses the ability of moving about in flowing water pond collects on the block of discharge outlet. sick fish body colour blackout, exophthalmos, abdominal part expand, and abdominal part and fin base portion are congested, and the gill is pale red, and 1 wire Mucous Stool has often been dragged at anus place.Cut fish belly visible ascites sometimes open, pylorospasm is hemorrhage, and liver spleen kidney heart abnormality is pale; In digestive tract, conventionally there is no food, and have milky or faint yellow mucus, these mucus are not solidified conventionally in the formalin of 5-10%.Postlarva in 3 months after the main infringement of IPNV virus salmon fishes starts to ingest.At height

Under density raising condition, to the juvenile fish of salmon, Squaliobarbus ourriculus class, be a kind of virosis of hyperinfection.Morbidity water temperature is generally 10-15 ℃.The larval rainbow in age in 10-20 week, in the time of water temperature 10-12 ℃, infection rate and mortality rate can reach 80-100%.Fingerling after 20 week age is not generally fallen ill, and can become lifelong carrier.By using Operand disinfecting drug or antiviral drugs, to aqueous solution, sterilization prevents and treats the current method of preventing and treating; The Chinese herbal medicine such as rhei,rhizoma pulveratum also have prevention effect to this disease.Chinese medicine or its extract, for antiviral, have certain superiority, as low in drug residue, so Study of Traditional Chinese Medicine antiviral drugs is necessary for the control of Salmons pancreatic necrosis disease.

 

summary of the invention:

The object of the invention is to overcome the shortcoming existing in prior art, provide a kind of proportioning simple, easy to use, IPNV is had to inhibitory action, sick for Salmons pancreatic necrosis specially, the strong diabetes involving middle-JIAO poison of nourishing liver and kidney, the pancreatic necrosis disease Chinese medicine composition of the treatment salmon that therapeutic effect is good, Squaliobarbus ourriculus.

To achieve these goals, the invention provides a kind of pancreatic necrosis disease Chinese medicine composition for the treatment of salmon, Squaliobarbus ourriculus, wherein, according to unit of weight, configure following each component:

Herba Scutellariae Barbatae, Radix Trichosanthis, each 100-150g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 60-80g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 40-60g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 20-30g, PVP-1 is 1-3g, plant blood cell clusterin 5-20mg.

Or, according to unit of weight, configure following each component:

Herba Scutellariae Barbatae, Radix Trichosanthis, each 100-120g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 60-70g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 40-50g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 20-25g, PVP-1 is 1-2g, plant blood cell clusterin 5-10mg.

Or, according to unit of weight, configure following each component:

Herba Scutellariae Barbatae, Radix Trichosanthis, each 120-150g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 70-80g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 50-60g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 25-30g, PVP-1 is 2-3g, plant blood cell clusterin 10-20mg.

The sick preparation method with Chinese medicine composition of pancreatic necrosis for the treatment of salmon, Squaliobarbus ourriculus is as follows:

By each crude drug component (Herba Scutellariae Barbatae in above-mentioned Chinese medicine composition, Radix Trichosanthis, Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, Rhizoma Zingiberis Recens, grind to form fine powder Radix Glycyrrhizae) respectively, mix, obtain mixture, mixture is joined in the water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out concentrating under reduced pressure and obtain extractum, extractum is joined in the alcoholic solution that the determining alcohol with respect to 2 ~ 3 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to concentrating under reduced pressure and remove ethanol, the mastic of acquisition is pulverized, and adds PVP-1 to be, after plant blood cell clusterin, mix gains and starch, hydroxy methocel mixes according to mass ratio 1:2:1, with 14 mesh sieves, granulates, and toasts 30 minutes, obtains granular medicine, big or small 0.5-1mm.

Use: this medicine can mix feeding in graininess fish material, according to the quality of 0.6:1, compares proportioning.

Further, also can be on this granule medicament basis of (being called drug core), i.e. the feedstuff of the parcel in appearance coating of this medicine, is prepared into and take the fish material that this medicine is core, is specially:

By normal feedstuff: (by bean cake 150g, fish flour 40g, wheat flour 400g, Semen Maydis powder 600g, salt 10g, vitamin 2g) add water and stir as water content 65%, add Sodium Hydroxymethyl Stalcs to be thickened to thick, drug core is repeatedly rolled across on viscous solution, after dry, roll again for a moment, normal feedstuff is wrapped on the outer wall of drug core, thickness is 0.3-0.5mm.After dry, be able to fish material.

The complex of the chemical constitution of described PVP-1: PVP and iodine

CTFA title: PVP-IOdide

Character: PVP-I is the complex of PVP and iodine, and antibacterial, virus, fungus, mycete and spore are had to stronger killing action.Stable, non-stimulated, completely water-soluble.

Specification:

Loss on drying % calcination residual value arsenic (ug/g) heavy metal (ug/g) nitrogen % iodide ion % available iodine %

≤8.0?≤0.1?≤1.5?≤20?9.5-11.5?≤6.6?9.0-12.0。

 

Advantage of the present invention is, proportioning is simple, easy to use, and IPNV is had to inhibitory action, sick for Salmons pancreatic necrosis specially, the strong diabetes involving middle-JIAO poison of nourishing liver and kidney, and therapeutic effect is good.

 

the specific embodiment:

Principle:

The present invention take direct deactivation, to suppress the Radix Paeoniae that viral Herba Scutellariae Barbatae, Radix Trichosanthis and Promote immunity reach antivirus action be monarch drug, use in a large number medicine as ministerial drug, adjuvant drug simultaneously and make medicine.As, purging liver-fire to medicine Radix Scutellariae, the Radix Rehmanniae; Liver heat removing dampness removing to medicine Radix Gentianae, Caulis Akebiae; Warming the liver circulation of qi promoting to medicine Fructus Evodiae, Fructus Foeniculi; Smooth the liver and regulate the spleen to medicine Fructus Toosendan, Pericarpium Citri Reticulatae Viride; Warming the kidney to promote diuresis to medicine Radix Achyranthis Bidentatae, Semen Plantaginis, be ministerial drug; Invigorating the spleen and replenishing QI to medicine Radix Codonopsis, the Radix Astragali; Lung-heat clearing and detoxification to medicine Radix Isatidis, Radix Scrophulariae; Circulation of qi promoting intestinal stasis relieving to medicine Fructus Aurantii, Massa Medicata Fermentata; Clearing gallbladder stomach function regulating to medicine Radix Bupleuri, Rhizoma Zingiberis Recens, be adjuvant drug, Radix Glycyrrhizae is for making medicine.Be aided with PVP-1, plant blood cell clusterin is strengthened therapeutic effect again.

 

Treatment contrast, select at random the salmon that infects pancreatic necrosis virus, be divided into 5 groups, every group 24, the traditional Radix Et Rhizoma Rhei Radix Et Rhizoma Rhei powder Chinese medicine powder of feeding is group three for group one, with 2% formalin or sodium hydrate aqueous solution (pH12.2) sterilization for what organize two, use plant blood cell anticoagulin, uses PVP-1 to treat as organizing four, uses the present invention for organizing five, under equal conditions, within 3 days, observe afterwards.

Antiviral experimental data:

Interior resisting virus experiment to salmon

Get fish, by body weight, divide 2 groups at random: viral infection matched group and intact animal's matched group, except Normal group, salmon is slightly anaesthetized, with the pancreatic necrosis virus (IPNV) of 15 LDSD, infect every 0.1ml, from infecting, start administration the previous day, viral infection matched group feeding every day of the present invention 2 times, continuous 5 days, the 6th day, after taking body weight, fix, dissect, get pancreas and calculate pancreas exponential quantity, calculate pancreas index suppression ratio

Heavy (the g)/body weight (g) * 100 of pancreas index=pancreas

Pancreas index suppression ratio=(virus control group pancreas Mean value of index-experimental group pancreas Mean value of index)/virus control group pancreas Mean value of index * 100%

Pancreas index is larger, represents that liver pathological changes degree is serious, (because hemorrhagic disease causes each internal organs hemorrhage, select internal organs as index, also can select lung to carry out lung assessment of indices)

Pancreatic tissue is fixed with 10% formaldehyde, ethanol series dehydration, and dimethylbenzene is transparent, paraffin embedding, slice thickness 4-5 micron, HE dyeing, with ordinary optical microscope, observe pancreatic tissue metamorphosis, select the antiviral group of PVP-1 as a comparison, can find out that successful of the present invention is better than PVP-1 group.

The impact (n=10) of pancreas checking (virus is observed)

Can find, of the present invention to viral suppression ratio apparently higher than directly using PVP-1.

 

The experiment of toxicity to rat:

Acute oral (LD50) > 500mg/kg,

Acute percutaneous (LD 50) > 2000mg/kg,

Belong to low toxicity.

 

Object lesson 1

Burnt Jia Yi plant, area 900 square meters, approximately 7 meters of the depth of waters, put salmon 7000 tails in a suitable place to breed, during 12 degrees Celsius of water temperatures, start morbidity dead fish successively in water, and every day, the quantity of dead fish was multiplied, and a day dead salmon reaches tail more than 500 at most.Detect disease fish, sick fish swimming imbalance, normal work vertically turned round and moved about, soon just sink under water, watching has a rest repeats again above moving about in a moment, until dead, generally from starting revolution, move about to death only 1～2 hour, the most obvious feature is pancreatic necrosis, pancreas bubble, islets of langerhans and all cells nearly all occur extremely, most necrocytosiss, particularly karyopycnosis, karyorrhexis is very remarkable, in the endochylema of some cell, there is inclusion body, be diagnosed as salmon pancreatic necrosis sick, use Herba Scutellariae Barbatae of the present invention, Radix Trichosanthis, each 150g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 80g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 60g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 30g, PVP-1 is 3g, plant blood cell clusterin 20mg.

By each crude drug component (Herba Scutellariae Barbatae in above-mentioned Chinese medicine composition, Radix Trichosanthis, Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, Rhizoma Zingiberis Recens, grind to form fine powder Radix Glycyrrhizae) respectively, mix, obtain mixture, mixture is joined in the water of 6 ~ 8 times of amounts and decoct, decoct 2 ~ 4 hours at every turn, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out concentrating under reduced pressure and obtain extractum, extractum is joined in the alcoholic solution that the determining alcohol with respect to 2 ~ 3 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, 4 ℃ of following standing over night of temperature, subsequent filtration, carries out concentrating under reduced pressure by filtrate and removes ethanol subsequently, the mastic obtaining is pulverized, adding PVP-1 is 3g, plant blood cell clusterin 20mg, gains and starch, hydroxy methocel mixes according to mass ratio 1:2:1, with 14 mesh sieves, granulates, and toasts 30 minutes, obtains granular medicine, big or small 0.5-1mm.

Above-mentioned consumption is the consumption of 100 fishes, is multiplied by multiple goes preparation according to the quantity of fish.

Use: this medicine can mix feeding in graininess fish material, according to the quality of 0.6:1, compares proportioning.

1 meal/day, continuous 6 days, the dead quantity of the 1st day salmon after medication was 464 tails, the 2nd day dead quantity 357 tails of salmon, the 3rd day dead 254 tails, the 4th day dead 152, the 5th day dead 62, salmon pancreatic necrosis do not occur afterwards more sick, and general mortality rate is 1.84%.

 

Example 2

Liu Jia plant 1700 square meters, put 12000 tails in a suitable place to breed, and 10 degrees Celsius of water temperatures are found Squaliobarbus ourriculus mortality, and dead fish body is black, eye is prominent, abdominal part expands or ascites, fin base or abdominal part are hemorrhage, and present situation feces has been dragged at anus place, is full of mucus in digestive tract.Find pancreatic necrosis, in the endochylema of cell, have inclusion body, infiltrate and also have inclusion body in the macrophage endochylema of pancreas, pancreas fatty tissue around also necroses, skeletal muscle generation vitreous degeneration; In the disease later stage, also there is degeneration, necrosis in the hemopoietic tissue of kidney and renal tubules, and pancreas focal necrosis, is peeled off at gastral mucosa generation degeneration, necrosis.Be diagnosed as pancreatic necrosis disease, use the present invention.

Herba Scutellariae Barbatae, Radix Trichosanthis, each 100g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 60g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 40g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 20g, PVP-1 is 1.5g, plant blood cell clusterin 10mg.

 

By each crude drug component (Herba Scutellariae Barbatae in above-mentioned Chinese medicine composition, Radix Trichosanthis, Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, Rhizoma Zingiberis Recens, grind to form fine powder Radix Glycyrrhizae) respectively, mix, obtain mixture, mixture is joined in the water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out concentrating under reduced pressure and obtain extractum, extractum is joined in the alcoholic solution that the determining alcohol with respect to 2 ~ 3 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to concentrating under reduced pressure and remove ethanol, the mastic obtaining is pulverized, adding PVP-1 is 1.5g, plant blood cell clusterin 10mg.Gains and starch, hydroxy methocel mix according to mass ratio 1:2:1, with 14 mesh sieves, granulate, and toast 30 minutes, obtain granular medicine, big or small 0.5-1mm.

On the basis of this granule medicament (being called drug core), i.e. the feedstuff of the parcel in appearance coating of this medicine, is prepared into and take the fish material that this medicine is core, is specially:

By normal feedstuff: (by bean cake 150g, fish flour 40g, wheat flour 400g, Semen Maydis powder 600g, salt 10g, vitamin 2g), adding water stirs as water content 65%, add Sodium Hydroxymethyl Stalcs and be thickened to thickly, drug core is repeatedly rolled across on viscous solution, roll again for a moment after dry, normal feedstuff is wrapped on the outer wall of drug core, and thickness is 0.3-0.5mm.After dry, be able to fish material.

This example is 100 Squaliobarbus ourriculus use, is multiplied by multiple goes preparation according to fish tail number.

1 meal/day, continuous 8 days, the dead quantity of the 1st day Squaliobarbus ourriculus after medication was 486 tails, the 2nd day dead quantity 402 tails of salmon, the 3rd day dead 312 tails, the 4th day dead 201 tails, the 5th day dead 126, the 6th day dead 37, after this there is no fish Mortality, general mortality rate is 1.30%.

 

Example 3,

Yan Jia plant 1100 square meters, put 10000 tails in a suitable place to breed, during 12 degrees Celsius of water temperatures, a large amount of salmons occur dead, and dead fish body is black, eye is prominent, abdominal part ascites, anus place have been dragged present situation feces, is full of mucus in digestive tract.Find pancreatic necrosis, pancreas fatty tissue around also necroses, and gastral mucosa generation degeneration, necrosis, peels off.Be diagnosed as salmon pancreatic necrosis sick, use the present invention.

Herba Scutellariae Barbatae, Radix Trichosanthis, each 120g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 70g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 30g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 25g, PVP-1 is 2g, plant blood cell clusterin 15mg.

By each crude drug component (Herba Scutellariae Barbatae in above-mentioned Chinese medicine composition, Radix Trichosanthis, Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, Rhizoma Zingiberis Recens, grind to form fine powder Radix Glycyrrhizae) respectively, mix, obtain mixture, mixture is joined in the water of 6 ~ 8 times of amounts and decoct, each decoction 2 ~ 4 hours, decoct 3 times, decocted rear filtration at every turn, filtrate is mixed, carry out concentrating under reduced pressure and obtain extractum, extractum is joined in the alcoholic solution that the determining alcohol with respect to 2 ~ 3 times of amounts of mixture quality is 90% ~ 95%, stir completely and dissolve, subsequently 4 ℃ of following standing over night of temperature, subsequent filtration, filtrate is carried out to concentrating under reduced pressure and remove ethanol, the mastic obtaining is pulverized, adding PVP-1 is 1.5g, plant blood cell clusterin 10mg.Gains and starch, hydroxy methocel mix according to mass ratio 1:2:1, with 14 mesh sieves, granulate, and toast 30 minutes, obtain granular medicine, big or small 0.5-1mm.

On the basis of this granule medicament (being called drug core), i.e. the feedstuff of the parcel in appearance coating of this medicine, is prepared into and take the fish material that this medicine is core, is specially:

By normal feedstuff: (by bean cake 150g, fish flour 40g, wheat flour 400g, Semen Maydis powder 600g, salt 10g, vitamin 2g) add water and stir as water content 65%, add Sodium Hydroxymethyl Stalcs to be thickened to thick, drug core is repeatedly rolled across on viscous solution, after dry, roll again for a moment, normal feedstuff is wrapped on the outer wall of drug core, thickness is 0.3-0.5mm.After dry, be able to fish material.

This example is 100 salmon use, is multiplied by multiple goes preparation according to fish tail number.

1 meal/day, continuous 8 days, the dead quantity of the 1st day salmon after medication was 392 tails, the 2nd day dead quantity 314 tails of salmon, the 3rd day dead 275 tails, the 4th day dead 178 tails, the 5th day dead 86, the 6th day dead 41, after this there is no fish Mortality, general mortality rate is 1.28%

To sum up, proportioning of the present invention is simple, easy to use, and sick for pancreatic necrosis specially, therapeutic effect is good, and pancreatic necrosis virus is had to deactivation, inhibitory action, the strong diabetes involving middle-JIAO poison of nourishing liver and kidney, and therapeutic effect is good.

Claims (3)

1. a pancreatic necrosis disease Chinese medicine composition for the treatment of salmon, Squaliobarbus ourriculus, is characterized in that, according to unit of weight, configures following each component:

Herba Scutellariae Barbatae, Radix Trichosanthis, each 100-150g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 60-80g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 40-60g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 20-30g, PVP-1 is 1-3g, plant blood cell clusterin 5-20mg.

2. the pancreatic necrosis disease Chinese medicine composition for the treatment of according to claim 1 salmon, Squaliobarbus ourriculus, is characterized in that, according to unit of weight, configures following each component:

Herba Scutellariae Barbatae, Radix Trichosanthis, each 100-120g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 60-70g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 40-50g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 20-25g, PVP-1 is 1-2g, plant blood cell clusterin 5-10mg.

3. the pancreatic necrosis disease Chinese medicine composition for the treatment of according to claim 1 salmon, Squaliobarbus ourriculus, is characterized in that, according to unit of weight, configures following each component:

Herba Scutellariae Barbatae, Radix Trichosanthis, each 120-150g of Radix Paeoniae, Radix Scutellariae, the Radix Rehmanniae, Radix Gentianae, Caulis Akebiae, Fructus Evodiae, Fructus Foeniculi, Fructus Toosendan, Pericarpium Citri Reticulatae Viride, Radix Achyranthis Bidentatae, each 70-80g of Semen Plantaginis, Radix Codonopsis, the Radix Astragali, Radix Isatidis, Radix Scrophulariae, Fructus Aurantii, Massa Medicata Fermentata, Radix Bupleuri, each 50-60g of Rhizoma Zingiberis Recens, Radix Glycyrrhizae 25-30g, PVP-1 is 2-3g, plant blood cell clusterin 10-20mg.