CN104178582A - 一种丙型肝炎病毒的试剂盒及其检测方法 - Google Patents

一种丙型肝炎病毒的试剂盒及其检测方法 Download PDF

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CN104178582A
CN104178582A CN201410261734.5A CN201410261734A CN104178582A CN 104178582 A CN104178582 A CN 104178582A CN 201410261734 A CN201410261734 A CN 201410261734A CN 104178582 A CN104178582 A CN 104178582A
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hepatitis
virus
primer
pcr reaction
kit
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江崇才
李作臻
仇彩霞
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    • C—CHEMISTRY; METALLURGY
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/706—Specific hybridization probes for hepatitis
    • C12Q1/707—Specific hybridization probes for hepatitis non-A, non-B Hepatitis, excluding hepatitis D
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

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Abstract

本发明公开一种丙型肝炎病毒的试剂盒，由红细胞裂解液、DNA提取液、检测体系PCR反应液和检测IL28Brs12980275多态性的引物组成,所述检测体系PCR反应液包括dNTP，用于PCR反应的Tag酶，缓冲反应液；所述检测IL28Brs12980275多态性的引物分为上下游，上游引物序列为GTAGGGGGAATCATACAGCATT；下游引物序列为GAGAGTTCTGGAGATTGCTTGC；该丙型肝炎病毒的试剂盒拥有灵敏度高，稳定性好，操作简单，成本低的优点。

Description

一种丙型肝炎病毒的试剂盒及其检测方法
技术领域
本发明涉及一种丙型肝炎病毒的试剂盒及其检测方法。
背景技术
丙型肝炎是丙型肝炎病毒(HCV)感染所引起的疾病，主要经血源性传播。临床表现有发热、消化道症状及肝功能异常等。与乙型肝炎类似，但较轻。多数病例呈亚临床型，慢性化程度较为严重，也可导致暴发性肝衰竭。目前临床检测丙型肝炎的方法有很多，主要分为血清胆红素测定，血清酶测定，蛋白代谢功能测验，凝血酶原时间及活动度测定，脂质代谢有关测验等等。目前所有的丙型肝炎病毒的试剂灵敏度不高，稳定性不强，并且部分成本高昂。
发明内容
本发明要解决的技术问题是提供一种灵敏度高，稳定性好，操作简单，成本低的丙型肝炎病毒的试剂盒。
为解决上述技术问题，本发明采用如下技术方案：一种丙型肝炎病毒的试剂盒，所述丙型肝炎病毒的试剂盒由红细胞裂解液、DNA提取液、检测体系PCR反应液和检测IL28Brs12980275多态性的引物组成,所述检测体系PCR反应液包括dNTP，用于PCR反应的Tag酶，缓冲反应液；所述检测IL28Brs12980275多态性的引物分为上下游，上游引物序列为GTAGGGGGAATCATACAGCATT；下游引物序列为GAGAGTTCTGGAGATTGCTTGC。
本发明的一种丙型肝炎病毒的试剂盒及其检测方法的有益效果是：灵敏度高，稳定性好，操作简单，成本低。
具体实施方式
为能进一步了解本发明的特征、技术手段及所达到的具体功能，下面以具体实施方式对本发明做详细描述。
实施例1：
一种丙型肝炎病毒的试剂盒，所述丙型肝炎病毒的试剂盒由红细胞裂解液、DNA提取液、检测体系PCR反应液和检测IL28Brs12980275多态性的引物组成,所述检测体系PCR反应液包括dNTP，用于PCR反应的Tag酶，缓冲反应液；所述检测IL28Brs12980275多态性的引物分为上下游，上游引物序列为GTAGGGGGAATCATACAGCATT；下游引物序列为GAGAGTTCTGGAGATTGCTTGC。
实验例：
实验对象：200名患丙型肝炎程度不同的患者。
实验方法：200名患者全部用本发明的丙型肝炎病毒的试剂盒检测。
实验结果：通过本发明的丙型肝炎病毒的试剂盒检测，所有患者都成功地检测出来丙型肝炎病毒。
由实验结果可知，使用本丙型肝炎病毒的试剂盒检测率很高，灵敏度高，稳定性好，操作简单，成本低。
以上已将本发明做一详细说明，以上所述，仅为本发明之较佳实施例而已，当不能限定本发明实施范围，即凡依本申请范围所作均等变化与修饰，皆应仍属本发明涵盖范围内。

Claims (1)

1.一种丙型肝炎病毒的试剂盒，其特征在于：所述丙型肝炎病毒的试剂盒由红细胞裂解液、DNA提取液、检测体系PCR反应液和检测IL28Brs12980275多态性的引物组成,所述检测体系PCR反应液包括dNTP，用于PCR反应的Tag酶，缓冲反应液；所述检测IL28Brs12980275多态性的引物分为上下游，上游引物序列为GTAGGGGGAATCATACAGCATT；下游引物序列为GAGAGTTCTGGAGATTGCTTGC。
CN201410261734.5A 2014-06-12 2014-06-12 一种丙型肝炎病毒的试剂盒及其检测方法 Pending CN104178582A (zh)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011076814A1 (en) * 2009-12-22 2011-06-30 Tibotec Pharmaceuticals Predictive value of il28b gene polymorphism combined with pretreatment serum ip-10 quantification for response to peginterferon and ribavirin is enhanced in comparison with any of these biomarkers alone
CN102277441A (zh) * 2011-09-07 2011-12-14 李艳 一种快速检测IL28B SNP rs12979860多态性的试剂盒
CN102816838A (zh) * 2012-07-06 2012-12-12 吉林艾迪康医学检验所有限公司 用于检测丙型肝炎患者il28b snp12980275多态性的试剂盒

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011076814A1 (en) * 2009-12-22 2011-06-30 Tibotec Pharmaceuticals Predictive value of il28b gene polymorphism combined with pretreatment serum ip-10 quantification for response to peginterferon and ribavirin is enhanced in comparison with any of these biomarkers alone
CN102277441A (zh) * 2011-09-07 2011-12-14 李艳 一种快速检测IL28B SNP rs12979860多态性的试剂盒
CN102816838A (zh) * 2012-07-06 2012-12-12 吉林艾迪康医学检验所有限公司 用于检测丙型肝炎患者il28b snp12980275多态性的试剂盒

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