CN104148124B - A kind of drop formation device for micro-fluidic chip - Google Patents
A kind of drop formation device for micro-fluidic chip Download PDFInfo
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- CN104148124B CN104148124B CN201410373307.6A CN201410373307A CN104148124B CN 104148124 B CN104148124 B CN 104148124B CN 201410373307 A CN201410373307 A CN 201410373307A CN 104148124 B CN104148124 B CN 104148124B
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Abstract
The present invention discloses a kind of drop formation device for micro-fluidic chip; this device is made up of hardware components and circuit part; it is characterized in that: described hardware components comprises plunger displacement pump, magnetic valve, micro-fluidic chip, hydrops bottle, described circuit part comprises driver, light-coupled isolation protection circuit, serial ports, parallel port, power supply, host computer.The drop size that apparatus of the present invention generate is less and uniformity good, and the condition generating drop is simple.It controls precisely drop size, and it is even less that droplet flow can reach micro updating, cost and low in energy consumption, to drop not damaged, simple to operate.
Description
Technical field
The present invention relates to computer technology and microfluidic control technical field, specifically a kind of drop formation device for micro-fluidic chip.
Background technology
Microflow control technique is one and relates in process unit processing and manufacturing technology and micro-meter scale space and manipulate fluid and the science of Treatment Analysis fluid information and behavior, and liquid drop microfluidic system is a new branch in micro-fluidic field in recent years.Drop is micro-fluidic is the technology generating and handle drop in closed microchannel network, it is a kind of high-throughout drop technique, each drop all can be regarded as independently reactor, compared with traditional biological chemical reaction, drop is micro-fluidic to be had sample and consumes low, response and control accuracy is high, reaction fast, without advantages such as cross pollutions.The system range of application producing and manipulate microlayer model is more and more wider, DNA analysis, crystallization of protein, the demarcation of cell fluorescence albumen etc. from machinery industries such as inkjet printings to life science.Micro-fluidic chip is the carrier of microflow control technique, micro-fluidic chip is at microscale spatial fluid administration, by the basic function micro in the laboratory such as biological, chemical on the chip of several square centimeters, chip lab (Lab-on-a-chip) can be also called.
The basis that microfluid system performance is ensured is microfluidic control technology, namely accurately also handles micro fluid efficiently.Current microfluidic control technology lacks on enough effective basis, to have microminiaturization, low cost, low-power consumption and the plurality of advantages platform technology such as simple and reliable concurrently.
Summary of the invention
For the deficiencies in the prior art, the technical problem to be solved in the present invention is: provide a kind of drop formation device for micro-fluidic chip.The size of this device controllable droplet, and size is less, control precisely, cost and power consumption is lower, simple to operate, it is good to generate the uniformity of droplet size.
The technical solution adopted for the present invention to solve the technical problems is: design a kind of drop formation device for micro-fluidic chip.This device is made up of hardware components and circuit part, it is characterized in that: described hardware components comprises plunger displacement pump, magnetic valve, micro-fluidic chip, hydrops bottle, described circuit part comprises driver, light-coupled isolation protection circuit, serial ports, parallel port, power supply, host computer;
Described plunger displacement pump is two, comprises piston, stepper motor, air inlet, gas outlet and air chamber, and piston one end is connected with stepper motor, and the other end is positioned at plenum interior, and air inlet and gas outlet lay respectively on air chamber two sides; Described magnetic valve is four, is respectively No. 1 magnetic valve, No. 2 magnetic valves, No. 3 magnetic valves, No. 4 magnetic valves; Described micro-fluidic chip comprises aquaporin, oily passage, Water-In-Oil drop forming region, fixture, described aquaporin and oily passage are small-sized reservoir, bottom surface all has a knitting wool pipeline to be communicated with Water-In-Oil drop forming region, and both capillary channels are together with meeting at before being communicated with Water-In-Oil drop forming region; Described fixture is reserved with guide hole, is arranged on above aquaporin, oily passage, Water-In-Oil drop forming region, it by aquaporin, oily passage, the sealing of Water-In-Oil drop forming region, and is connected with other parts with conduit; Described hydrops bottle is two, comprise water-storing bottle and oil storage bottle, bottle is closed structure, two conduits bottle stopper are equipped with, one higher than liquid level, one is immersed in liquid level, and the conduit other end higher than liquid level is connected with the gas outlet of plunger displacement pump respectively, the conduit in liquid level that is immersed in of water-storing bottle is connected with the aquaporin of micro-fluidic chip, and the conduit in liquid level that is immersed in of oil storage bottle is connected with the oily passage of micro-fluidic chip; Described No. 1 magnetic valve and No. 4 magnetic valves are arranged on the admission line of the air inlet of two plunger displacement pumps respectively, and No. 2 magnetic valves are arranged on the conduit between plunger displacement pump and water-storing bottle, and No. 3 magnetic valves are arranged on the conduit between plunger displacement pump and oil storage bottle;
The stepper motor of described plunger displacement pump is all connected with actuator electrical; driver Single port is connected with power supply; another port is connected with host computer by serial ports; light-coupled isolation protection circuit is connected with No. 1-No. 4 magnetic valves, parallel port and power supply respectively by wire, and light-coupled isolation protection circuit is connected with host computer by parallel port.
Compared with prior art, the invention has the beneficial effects as follows: the drop size that apparatus of the present invention generate is less and uniformity good, the condition generating drop is simple.It controls precisely drop size, and it is even less that droplet flow can reach micro updating, cost and low in energy consumption, to drop not damaged, simple to operate.
Accompanying drawing explanation
Fig. 1 is the piping diagram of the present invention for a kind of embodiment of drop formation device of micro-fluidic chip.
Fig. 2 is the circuit connection diagram of the present invention for a kind of embodiment of drop formation device of micro-fluidic chip.
Fig. 3 is the microscope figure of the present invention for the generation drop of a kind of embodiment of drop formation device of micro-fluidic chip.
Detailed description of the invention
The present invention is introduced further below in conjunction with specific embodiment and accompanying drawing.Embodiment is only further illustrate of the present invention and explain below, does not limit the protection domain of the application's claim with this.
The drop formation device (see Fig. 1-2) that the present invention is used for micro-fluidic chip is made up of hardware components and circuit part, described hardware components comprises plunger displacement pump 1, magnetic valve 2, micro-fluidic chip 8, hydrops bottle 9, and described circuit part comprises driver 3, light-coupled isolation protection circuit 4, serial ports 5, parallel port 6, power supply 7, host computer 10;
Described plunger displacement pump 1 is two, comprises piston 11, stepper motor 12, air inlet 13, gas outlet 14 and air chamber 15, and piston 11 one end is connected with stepper motor 12, and the other end is positioned at plenum interior, and air inlet 13 and gas outlet 12 lay respectively on air chamber 15 two sides; Described magnetic valve 2 is four, is respectively No. 1 magnetic valve 21, No. 2 magnetic valves 22, No. 3 magnetic valves 23, No. 4 magnetic valves 24; Described micro-fluidic chip 8 comprises water channel 81, oily passage 82, Water-In-Oil drop forming region 83, fixture 84, described water channel 81 and oily passage 82 are small-sized reservoir, bottom surface all has a knitting wool pipeline to be communicated with Water-In-Oil drop forming region 83, and both capillary channels are together with meeting at before being communicated with Water-In-Oil drop forming region 83; Described fixture 84 is reserved with guide hole, and be arranged on above water channel 81, oily passage 82, Water-In-Oil drop forming region 83, water channel 81, oily passage 82, Water-In-Oil drop forming region 83 seal, and are connected with other parts with conduit by it; Described hydrops bottle 9 is two, comprise water-storing bottle 91 and oil storage bottle 92, bottle is closed structure, two conduits bottle stopper are equipped with, one higher than liquid level, one is immersed in liquid level, and the conduit other end higher than liquid level is connected with the gas outlet 14 of plunger displacement pump 1 respectively, the conduit in liquid level that is immersed in of water-storing bottle 91 is connected with the water channel 81 of micro-fluidic chip 8, and the conduit in liquid level that is immersed in of oil storage bottle 92 is connected with the oily passage 82 of micro-fluidic chip 8; Described No. 1 magnetic valve 21 and No. 4 magnetic valves 24 are arranged on the admission line of air inlet 13 of two plunger displacement pumps 1 respectively, No. 2 magnetic valves 22 are arranged on the conduit between plunger displacement pump 1 and water-storing bottle 91, and No. 3 magnetic valves 23 are arranged on the conduit between plunger displacement pump 1 and oil storage bottle 92;
The stepper motor 12 of described plunger displacement pump 1 is all electrically connected with driver 3; driver 3 Single port is connected with power supply 7; another port is connected with host computer 10 by serial ports 5; light-coupled isolation protection circuit 4 is connected with No. 1-No. 4 magnetic valves 2, parallel port 6 and power supply 7 respectively by wire, and light-coupled isolation protection circuit 4 is connected with host computer 10 by parallel port 6.
Described power supply 7 one termination civil power, the other end can provide the power supply of 5V and 24V.
Described light-coupled isolation protection circuit 4 mainly comprises 3 parts: ground, 5V and 24V; 5V part has 5 to arrange pins, a 5V connecing power supply, and all the other 4 data bit connecing parallel port 6 are 0V or 5V; If the corresponding data position of parallel port 6 exports 1, corresponding row's pin place is 5V, and exporting 0 is 0V; Ground part has 5 to arrange pins, a GND connecing parallel port 6,4 GND connecing No. 1-No. 4 magnetic valves 2 respectively; 24V part has 5 to arrange pins, and a 24V connecing power supply, all the other 4 connect No. 1-No. 4 magnetic valves 2 respectively, are 0V or 24V.
The model of described driver is the UIM241C04P-MS of Beijing Jia Lande Science and Technology Ltd..
The operation principle of apparatus of the present invention and the course of work: after power module 7 is powered, driver 3 receives the control instruction that host computer 10 is sent by serial ports 5, and then the piston 11 controlling plunger displacement pump 1 has moved up and down air inlet and has given vent to anger.Gas sucks from the admission line of two plunger displacement pumps 1 through No. 1 magnetic valve 21, No. 4 magnetic valve 24 sides respectively, and the air inlet 13 entering plunger displacement pump 1 from opposite side enters air chamber 15, and the piston 11 of plunger displacement pump is walked downwards, and when piston 11 moves to lowermost end, air inlet completes.Each plunger displacement pump is by two solenoid control, after air inlet completes, No. 1 magnetic valve 21, No. 4 magnetic valves 24 cut out, No. 2 magnetic valves 22, No. 3 magnetic valves 23 are opened, the piston 11 of plunger displacement pump 1 is upwards walked, and gas enters No. 2 magnetic valves 22, No. 3 magnetic valves 23 and is connected with water-storing bottle 91, oil storage bottle 92 respectively respectively through conduit through the gas outlet 14 of plunger displacement pump 1.Water-storing bottle 91 and oil storage bottle 92 are equipped with material dispersed with continuous phase material respectively, under gas pressure effect, material dispersed water channel 81, the oily passage 82 being squeezed into micro-fluidic chip 8 with continuous phase material respectively, and water in oil drop is formed in Water-In-Oil drop forming region 83.Host computer 10 controls the rate travel of plunger displacement pump 1, and then controls the size and shape forming drop.Host computer 10 carrys out the opening and closing of Controlling solenoid valve 2 by the data bit controlling parallel port 6, for preventing parallel port 6 to be burned, adds light-coupled isolation protection circuit 4.
Circuit control principle is as follows: a data bit of parallel port 6 controls the opening and closing of a magnetic valve, due to the open and-shut mode of 4 magnetic valves will be controlled, so need 4 in the data bit of 8, parallel port, the corresponding data bit in parallel port 6 exports the opening and closing that 0 or 1 determines corresponding magnetic valve: when data bit is that the voltage that 0 corresponding magnetic valve obtains 24V is opened, be that 0V closes when data bit is 1 corresponding solenoid voltage.The output of the data bit of parallel port 6 is controlled by host computer 10.
Embodiment 1
The present embodiment utilizes said apparatus, and water-storing bottle 91 is equipped with 40mt% distilled water and 60mt% glycerine mixed solution (material dispersed), and oil storage bottle 92 is equipped with silicone oil and adds the span80 (continuous phase material) of 0.2mt%.The stroke resolution ratio of institute's use plunger displacement pump walks for 0.00625mm/, and regulate material dispersed and velocity ratio that is continuous phase material to be 1:3 by host computer, the plunger displacement pump speed wherein controlling decentralized photo is 2 steps/s, and the plunger displacement pump speed controlling continuous phase is 6 steps/s.Utilize existing chip to generate symmetrical liquid drop that diameter is about 50 μm, experimental result is see Fig. 3.Reduce the diameter of chip channel if continue, more small drop can be generated.Through many experiments, the droplet size that experimental result generates is even, demonstrates the reliability of this device.
The present invention does not address part and is applicable to prior art.
Claims (4)
1. the drop formation device for micro-fluidic chip, this device is made up of hardware components and circuit part, it is characterized in that: described hardware components comprises plunger displacement pump, magnetic valve, micro-fluidic chip, hydrops bottle, described circuit part comprises driver, light-coupled isolation protection circuit, serial ports, parallel port, power supply, host computer;
Described plunger displacement pump is two, comprises piston, stepper motor, air inlet, gas outlet and air chamber, and piston one end is connected with stepper motor, and the other end is positioned at plenum interior, and air inlet and gas outlet lay respectively on air chamber two sides; Described magnetic valve is four, is respectively No. 1 magnetic valve, No. 2 magnetic valves, No. 3 magnetic valves, No. 4 magnetic valves; Described micro-fluidic chip comprises aquaporin, oily passage, Water-In-Oil drop forming region, fixture, described aquaporin and oily passage are small-sized reservoir, bottom surface all has a knitting wool pipeline to be communicated with Water-In-Oil drop forming region, and both capillary channels are together with meeting at before being communicated with Water-In-Oil drop forming region; Described fixture is reserved with guide hole, is arranged on above aquaporin, oily passage, Water-In-Oil drop forming region, it by aquaporin, oily passage, the sealing of Water-In-Oil drop forming region, and is connected with other parts with conduit; Described hydrops bottle is two, comprise water-storing bottle and oil storage bottle, bottle is closed structure, two conduits bottle stopper are equipped with, one higher than liquid level, one is immersed in liquid level, and the conduit other end higher than liquid level is connected with the gas outlet of plunger displacement pump respectively, the conduit in liquid level that is immersed in of water-storing bottle is connected with the aquaporin of micro-fluidic chip, and the conduit in liquid level that is immersed in of oil storage bottle is connected with the oily passage of micro-fluidic chip; Described No. 1 magnetic valve and No. 4 magnetic valves are arranged on the admission line of the air inlet of two plunger displacement pumps respectively, and No. 2 magnetic valves are arranged on the conduit between plunger displacement pump and water-storing bottle, and No. 3 magnetic valves are arranged on the conduit between plunger displacement pump and oil storage bottle;
The stepper motor of described plunger displacement pump is all connected with actuator electrical; driver Single port is connected with power supply; another port is connected with host computer by serial ports; light-coupled isolation protection circuit is connected with No. 1-No. 4 magnetic valves, parallel port and power supply respectively by wire, and light-coupled isolation protection circuit is connected with host computer by parallel port.
2. the drop formation device for micro-fluidic chip according to claim 1, is characterized in that: the model of described driver is UIM241C04P-MS.
3. the drop formation device for micro-fluidic chip according to claim 1, is characterized in that: described power supply one termination civil power, the other end can provide the power supply of 5V and 24V.
4. the drop formation device for micro-fluidic chip according to claim 1, is characterized in that: described light-coupled isolation protection circuit mainly comprises 3 parts: ground, 5V and 24V; 5V part has 5 to arrange pins, a 5V connecing power supply, all the other 4 data bit connecing parallel port; Ground part has 5 to arrange pins, a GND connecing parallel port, 4 GND connecing No. 1-No. 4 magnetic valves respectively; 24V part has 5 to arrange pins, and a 24V connecing power supply, all the other 4 connect No. 1-No. 4 magnetic valves respectively.
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| CN105013548B (en) * | 2015-07-30 | 2016-08-24 | 河北工业大学 | A kind of microfluidic chip liquid drop generating means |
| CN106215989A (en) * | 2016-08-22 | 2016-12-14 | 东南大学 | Device prepared by a kind of microemulsion rapid, high volume |
| CN106885762A (en) * | 2016-12-29 | 2017-06-23 | 哈尔滨工业大学 | Closed loop liquid drop microfluidic system based on capacitance detecting |
| CN108514895A (en) * | 2018-03-01 | 2018-09-11 | 北京天健惠康生物科技有限公司 | A kind of microlayer model generates and monitoring device |
| CN110523448B (en) * | 2019-09-03 | 2022-03-18 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Droplet preparation system and preparation method |
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| GB1437738A (en) * | 1973-06-28 | 1976-06-03 | American Hospital Supply Corp | Drop dispensing apparatus |
| DE10158645C2 (en) * | 2001-11-22 | 2003-12-04 | Jerini Ag | Tub-shaped incubation vessel |
| CN101816906A (en) * | 2009-02-26 | 2010-09-01 | 希森美康株式会社 | Reagent preparing device, specimen measuring device and reagent preparing method |
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