CN112007705A - Micro-droplet generating device - Google Patents
Micro-droplet generating device Download PDFInfo
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- CN112007705A CN112007705A CN202010931507.4A CN202010931507A CN112007705A CN 112007705 A CN112007705 A CN 112007705A CN 202010931507 A CN202010931507 A CN 202010931507A CN 112007705 A CN112007705 A CN 112007705A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/0241—Drop counters; Drop formers
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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Abstract
The invention provides a micro-droplet generating device, which comprises a substrate, wherein a control component, an air supply component and an air pressure regulating component are fixedly connected to the substrate, the air supply component comprises an air bottle, an air pump, an air release valve and a first pressure sensor are connected to the air bottle, the air pressure regulating component comprises an air storage bottle with a plurality of independent air cavities, the air bottle and the air cavities are selectively communicated through air cavity communication electromagnetic valves, a second pressure sensor, an oil phase electromagnetic valve and a water phase electromagnetic valve which are mutually connected in parallel through pipelines are connected to the wall of each air cavity, and the control component is configured to control the air release valve or the oil phase electromagnetic valve and the water phase electromagnetic valve to generate air release action according to pressure values monitored by the first pressure sensor and the second pressure sensor in real time when the pressure values exceed. The micro-droplet generation device can realize secondary monitoring and pressure-stabilizing regulation and control on the gas pressure in the gas path, prevent overlarge gas pressure fluctuation and further ensure the stability of micro-droplet generation.
Description
Technical Field
The invention belongs to the technical field of microfluidic chips, and particularly relates to a micro-droplet generating device.
Background
Micro-droplet microfluidics (droplet-based microfluidics) is a technology developed in recent years on microfluidic chips to manipulate micro-volumes of liquid. The micro-droplet technology is a micro-nano technology for dividing and separating continuous fluid into droplets with discrete nano-scale and lower volumes by utilizing the interaction between flow shearing force and surface tension in a micro-scale channel.
Up to now, the micro-droplet type has mainly two types of gas-liquid phase droplets and liquid-liquid phase droplets. Gas-liquid phase droplets have limited their use due to their tendency to volatilize and cause cross-contamination in the microchannels. The liquid-liquid phase droplets are further classified into oil-in-water (O/W), water-in-oil (W/O), oil-in-water-in-oil (O/W/O), water-in-oil-in-water (W/O/W), and the like according to the difference between the continuous phase and the dispersed phase, which can overcome the disadvantages of droplet volatilization, cross contamination, and the like, and is therefore the focus of the development of the microfluidic droplet technology. Wherein the water-in-oil (W/O) micro-droplet generation principle is as follows: two mutually insoluble liquids, for example, one is oil phase and the other is water phase, after the oil phase and the water phase enter the micro-channel simultaneously, the water phase is in micro volume (10) under the action of the micro-channel-15～10-9L) the features are distributed in the oil phase in the form of a series of discrete microdroplets. Each droplet acts as a microreactor to accomplish a set of chemical or biological reactions. The micro-droplets used for screening have the following advantages:
1) the sample consumption is very little, and the screening cost is greatly reduced;
2) the generated micro-droplets have wider size range, controllable size and better monodispersity, and have low requirement on the wettability of wall materials, so the micro-droplets are widely concerned and applied;
3) the liquid drops are wrapped by the oil, no matter is exchanged with the outside, the reaction conditions in the liquid drops are stable, and the result is reliable.
Therefore, the method has important and wide application prospects in the aspects of drug screening, micro-nano material synthesis, enzyme reaction analysis and the like.
For a microfluidic chip, the number, size and uniformity of generated micro-droplets are important parameters for measuring the performance index of the microfluidic chip. The existing micro-fluidic chip mostly adopts a control mode of a micro-injection pump, and because the existing micro-injection pump has limited air pressure control precision and relatively low qualification rate of generated micro-droplets, the micro-droplets generated by controlling the micro-injection pump can not meet increasingly high-standard experimental requirements, and the micro-fluidic chip is provided based on the defects in the prior art.
Disclosure of Invention
Therefore, the technical problem to be solved by the present invention is to provide a micro-droplet generating device, wherein the gas supply component and the gas cavity are provided with pressure sensors, so that secondary real-time monitoring and pressure-stabilizing regulation and control of the gas pressure in the gas path can be realized, excessive gas pressure fluctuation is prevented, and the stability of micro-droplet generation is further ensured.
In order to solve the above problems, the present invention provides a micro-droplet generating device, comprising a substrate, wherein a control unit, a gas supply unit and a gas pressure regulating unit are fixedly connected to the substrate, the gas supply unit comprises a gas cylinder fixedly connected to the substrate, the gas cylinder is fixedly connected to a gas pump, a gas release valve and a first pressure sensor capable of monitoring gas pressure in the gas cylinder in real time, the gas pressure regulating unit comprises a gas cylinder having a plurality of independent gas chambers, the gas cylinder and the gas chambers are selectively communicated through gas chamber communicating solenoid valves, a second pressure sensor for detecting gas pressure in the corresponding gas chamber in real time is fixedly connected to a chamber wall of each gas chamber, and an oil phase solenoid valve and a water phase solenoid valve are connected in parallel to each other via pipelines, the control unit is configured to be capable of monitoring the pressure value in real time according to the first pressure sensor and the second pressure sensor, and when the pressure value exceeds the pressure preset threshold range, controlling the air release valve or the oil phase electromagnetic valve and the water phase electromagnetic valve to generate air release action.
Preferably, the control component is further configured to control the air pump to operate when the pressure value is lower than a preset pressure threshold range, or control the oil-phase electromagnetic valve and the water-phase electromagnetic valve to communicate the air cavity with the chip component located downstream of the air cavity; or, the gas cylinder is further provided with an air filter and a one-way valve, wherein the air filter is used for filtering the inlet gas of the gas pump, and the one-way valve is used for preventing the reverse flow of the gas between the gas pump and the gas cylinder.
Preferably, the micro-droplet generating device further comprises a chip component and a chip moving component, wherein the chip moving component comprises a linear displacement driving component and a driven plate, the chip component is placed on the driven plate, and the linear displacement driving component can drive the driven plate to generate linear reciprocating motion.
Preferably, the substrate is further provided with two displacement guide columns, the two displacement guide columns are respectively located on two sides of the chip moving component parallel to the moving direction of the chip moving component, two side faces of the driven plate corresponding to the two displacement guide columns are respectively provided with a sliding groove, the displacement guide columns correspond to the sliding grooves and are provided with a sliding block, and the sliding block is inserted into the corresponding sliding groove in a sliding manner.
Preferably, the slide groove includes a first horizontal guide section, a second horizontal guide section, and a transition section between the first horizontal guide section and the second horizontal guide section, the first horizontal guide section is lower than the second horizontal guide section in height, the chip component is in a micro-droplet non-generation position when the slider is in the first horizontal guide section, and the chip component is in a micro-droplet generation position when the slider is in the second horizontal guide section.
Preferably, the chip component includes a plurality of chip bodies arranged in parallel, and a liquid storage portion, a micro-droplet generation portion and a collection portion are configured on the chip bodies, wherein the liquid storage portion includes an oil phase liquid storage tank and a water phase liquid storage tank, the oil phase liquid storage tank and the water phase liquid storage tank are selectively communicated with the air cavity through the oil phase electromagnetic valve and the water phase electromagnetic valve respectively, and under the action of the pressure gas in the air cavity, the oil phase in the oil phase liquid storage tank and the water phase in the water phase liquid storage tank generate micro-droplets at the micro-droplet generation portion and are conveyed into the collection portion.
Preferably, the chip body is provided with a sealing cover, and the sealing cover is correspondingly arranged around the groove openings of the oil phase liquid storage tank and the water phase liquid storage tank in a surrounding mode, so that each oil phase liquid storage tank and each water phase liquid storage tank are sealed and independent mutually.
Preferably, the micro-droplet generating part includes a cross port including two oil phase ports corresponding to the oil phase reservoir, an aqueous phase port corresponding to the aqueous phase reservoir, and an output port corresponding to the collecting part, the output port communicating with the collecting part through a channel.
Preferably, still be provided with two support post on the base plate, two the support post is in respectively the chip removes the part and is on a parallel with its moving direction's both sides, the air feed part erects in two form the portal structure on the support post, work as when the chip part is in little liquid droplet generating position, the gas outlet of air feed part can with the pressurization mouth intercommunication that oil phase reservoir and aqueous phase reservoir have respectively.
Preferably, the linear displacement driving part comprises a slide rail, a motor fixing adapter, a lead screw fixing plate, a lead screw motor and a photoelectric sensor, wherein the lead screw fixing plate is fixedly connected with the substrate, a lead screw in the lead screw motor is fixedly connected with the lead screw fixing plate, a motor shell in the lead screw motor is fixedly connected with the slide rail through the motor fixing adapter, the slide rail is connected with the driven plate, and the photoelectric sensor is used for detecting and feeding back the real-time position of the lead screw motor. .
The micro-droplet generating device provided by the invention realizes real-time monitoring on the pressure of the pressure gas in the gas cylinder and the gas cavity through the first pressure sensor and the second pressure sensor respectively, when the gas pressure at the corresponding position exceeds the corresponding preset threshold range, the air release valve is controlled to release air in time or the oil phase electromagnetic valve and the water phase electromagnetic valve are controlled to release air, namely, air release action is generated, thereby ensuring that the pressure of the pressure gas in the micro-droplet generating device does not exceed the range of the preset threshold value, preventing the pressure fluctuation of the pressure gas from being overlarge through the real-time monitoring and adjustment of the pressure at the two sides, and then the sample introduction rate of the reagent in the micro-droplet generation part is accurately controlled, the stability of micro-droplet generation is ensured, high-precision automatic operation is realized, each group of generated micro-droplets to be detected are ensured to be in a mutually discrete state, and the use amount of the reagent is saved.
Drawings
Fig. 1 is a schematic system structure diagram of a micro-droplet generating device according to an embodiment of the present invention;
FIG. 2 is a schematic view showing a connection structure of the gas supply unit of FIG. 1;
FIG. 3 is a schematic perspective view of a droplet generator according to an embodiment of the present invention
FIG. 4 is a schematic diagram of the gas path component of FIG. 3;
FIG. 5 is a schematic top view of the chip body shown in FIG. 3;
FIG. 6 is a schematic perspective exploded view of the chip body in FIG. 3;
FIG. 7 is a schematic diagram of a process for generating micro-droplets on a chip body according to an embodiment of the present invention;
fig. 8 is a structural view of the follower plate of fig. 3 having a slide groove, and schematically shows a displacement direction in which the linear displacement driving part drives the follower plate to follow.
Fig. 9 is a schematic view of the linear displacement driving unit of fig. 3.
The reference numerals are represented as:
1. a control component; 11. an instruction output section; 12. a gas path control part; 13. a transmission control part; 2. a gas path component; 21. a gas supply part; 211. an air filter; 212. an air pump; 213. a one-way valve; 214. a gas cylinder; 215. a gas release valve; 216. a first pressure sensor; 22. an air pressure adjusting member; 221. a second pressure sensor; 222. an air cavity; 223. the air cavity is communicated with the electromagnetic valve; 224. an oil phase solenoid valve; 225. a water phase electromagnetic valve; 3. a chip moving part; 31. a linear displacement drive member; 311. a slide rail; 312. the motor is fixed with the adapter; 313. a screw rod fixing plate; 314. a screw motor; 315. a photosensor; 32. a driven plate; 4. a chip component; 41. a chip body; 411. a liquid storage part; 4111. an oil phase reservoir; 4112. a water phase reservoir; 412. a micro-droplet generating section; 4121. a cross port; 413. a collecting section; 414. a sealing cover; 100. a substrate; 101. a displacement guide upright post; 102. a chute; 1021. a first horizontal guide section; 1022. a second horizontal guide section; 1023. a transition section; 103. and supporting the upright post.
Detailed Description
Referring to fig. 1 to 9 in combination, according to an embodiment of the present invention, a micro-droplet generating apparatus is provided, which includes a substrate 100, a control component 1, a gas supply component 21 and a gas pressure regulating component 22 (both constituting a gas path component 2) are fixedly connected to the substrate 100, the gas supply component 21 includes a gas cylinder 214 fixedly connected to the substrate 100, a gas pump 212, a gas release valve 215 and a first pressure sensor 216 capable of monitoring gas pressure in the gas cylinder 214 in real time are fixedly connected to the gas cylinder 214, the gas pressure regulating component 22 includes a gas cylinder having a plurality of independent gas chambers 222, the gas cylinder 214 and the gas chambers 222 are selectively communicated through gas chamber communication solenoid valves 223, a second pressure sensor 221 for detecting gas pressure in the corresponding gas chambers 222 in real time and an oil phase solenoid valve 224, which are connected in parallel to each other, are fixedly connected to the chamber wall of each gas chamber 222, The water phase solenoid valve 225, and the control component 1 is configured to be capable of controlling the air release valve 215 or the oil phase solenoid valve 224 and the water phase solenoid valve 225 to generate air release action according to the pressure values monitored by the first pressure sensor 216 and the second pressure sensor 221 in real time, and when the pressure values exceed a preset pressure threshold range. In the technical scheme, the pressure of the pressure gas in the gas cylinder 214 and the gas cavity 222 is respectively monitored in real time by the first pressure sensor 216 and the second pressure sensor 221, when the gas pressure at the corresponding position exceeds the corresponding preset threshold range, the air release valve 215 is controlled to release air in time or the oil phase electromagnetic valve 224 and the water phase electromagnetic valve 225 are controlled to release air, namely, air release action is generated, thereby ensuring that the pressure of the pressure gas in the micro-droplet generating device does not exceed the range of the preset threshold value, preventing the pressure fluctuation of the pressure gas from being overlarge through the real-time monitoring and adjustment of the pressure at the two sides, and then the sample introduction rate of the reagent in the micro-droplet generation part is accurately controlled, the stability of micro-droplet generation is ensured, high-precision automatic operation is realized, each group of generated micro-droplets to be detected are ensured to be in a mutually discrete state, and the use amount of the reagent is saved. The air chamber communicating solenoid valve 223 is located on the pipeline between the air outlet pipe of the air bottle 214 and the air chamber 222 to realize the communicating control of the control component 1 on the air bottle 214 and the air chamber 222, and the corresponding air chamber communicating solenoid valve 223 is correspondingly arranged in each air pressure regulating component 22 to ensure the independent control on each air pressure regulating component 22.
Preferably, the control component 1 is further configured to control the operation of the air pump 212 when the pressure value is lower than a preset pressure threshold range, or control the oil-phase solenoid valve 224 and the water-phase solenoid valve 225 to communicate the air cavity 222 with the chip component 4 located downstream of the air channel thereof, in this technical solution, the first pressure sensor 216 and the second pressure sensor 221 monitor the pressure values of the pressure gas in the gas cylinder 214 and the air cavity 222 in real time and control the operation of the air pump 212 when the pressure values are lower than the preset pressure threshold range, or control the oil-phase solenoid valve 224 and the water-phase solenoid valve 225 to communicate the air cavity 222 with the chip component 4 located downstream of the air channel thereof to ensure that the pressure values of the corresponding parts can meet the requirements, which can further ensure the pressure stability of the pressure gas, prevent the pressure from fluctuating too much, that is to ensure that the pressure gas is always within the preset pressure, further ensure the stability of the micro-droplets, and further ensure that the generated micro-droplets are more uniform in size and are not easy to break. The gas bottle 214 is further provided with an air filter 211 and a one-way valve 213, wherein the air filter 211 is used for filtering the intake air of the gas pump 212, and the one-way valve 213 is used for preventing the reverse flow of the gas between the gas pump 212 and the gas bottle 214.
Specifically, as shown in fig. 1, the control component 1 includes an instruction output component 11, an air path control component 12, and a transmission control component 13, where the instruction output component 11 outputs a corresponding control instruction, and further, the air path control component 12 and the transmission control component 13 implement on-off control of each electromagnetic valve in the droplet generating device and position control of the chip moving component 3, and the like.
In some embodiments, the micro-droplet generating apparatus further comprises a chip component 4 and a chip moving component 3, the chip moving component 3 comprises a linear displacement driving component 31 and a driven plate 32, the chip component 4 is placed on the driven plate 32, and the linear displacement driving component 31 can drive the driven plate 32 to generate a linear reciprocating motion.
Further, the substrate 100 is further provided with two displacement guide columns 101, the two displacement guide columns 101 are respectively located on two sides of the chip moving component 3 parallel to the moving direction of the chip moving component, two side surfaces of the driven plate 32 corresponding to the two displacement guide columns 101 are respectively provided with sliding grooves 102, the displacement guide columns 101 corresponding to the sliding grooves 102 are provided with sliding blocks (not shown in the figure), and the sliding blocks are slidably inserted into the corresponding sliding grooves 102, so that the stability of linear displacement of the driven plate 32 can be ensured. Preferably, the chute 102 comprises a first horizontal guiding section 1021, a second horizontal guiding section 1022 and a transition section 1023 between the first horizontal guiding section 1021 and the second horizontal guiding section 1022, the first horizontal guiding section 1021 is lower than the second horizontal guiding section 1022, the chip component 4 is in the microdroplet non-generation position when the slider is in the first horizontal guiding section 1021, and the chip component 4 is in the microdroplet generation position when the slider is in the second horizontal guiding section 1022, in this technical solution, by linearly displacing the driven plate 32, a displacement difference can be generated in the height direction, and the presence of the displacement difference facilitates the air outlet of the oil phase air supply component 21 to be communicated with the pressurization ports of the oil phase reservoir 4111 and the water phase reservoir 4112 respectively when the chip component 4 is in the microdroplet generation position, therefore, the working motion requirements of the driven plate 32 in the horizontal direction and the height direction (vertical direction) can be realized only by one set of linear displacement driving part 31, the structure of the device is greatly simplified, and the manufacturing cost is reduced.
In addition, the linear displacement driving component 31 drives the driven plate 32 to generate corresponding motion under the control of the control component 1, for example, the control component 1 controls the motor of the linear displacement driving component 31 to move according to the feedback of the next step of the chip component 4, and meanwhile, the driven plate 32 moves to a corresponding position in a driven manner, so that the liquid storage part 411, the oil phase electromagnetic valve 224 and the water phase electromagnetic valve 225 can be controlled to be closed and opened in the process, the precision is high in the motion process, the adjustment adaptability is strong, a plurality of the linear displacement driving components 31 can be rapidly integrated, and different demand scenes can be met.
In some embodiments, the chip component 4 includes a plurality of chip bodies 41 arranged in parallel, the chip body 41 is configured with a liquid storage portion 411, a droplet generation portion 412 and a collection portion 413, wherein the liquid storage portion 411 includes an oil phase liquid storage tank 4111 and a water phase liquid storage tank 4112, the oil phase liquid storage tank 4111 and the water phase liquid storage tank 4112 are selectively communicated with the air chamber 222 through the oil phase solenoid valve 224 and the water phase solenoid valve 225, respectively, and under the action of the pressure gas in the air chamber 222, the oil phase in the oil phase liquid storage tank 4111 and the water phase in the water phase liquid storage tank 4112 generate droplets at the droplet generation portion 412 and are conveyed to the collection portion 413. The oil phase reservoir 4111 and the water phase reservoir 4112 are arranged on the chip body at the same time and are communicated with the oil phase electromagnetic valve 224 and the water phase electromagnetic valve 225 correspondingly, so that full-automatic operation of a micro-droplet generation process is realized.
In some embodiments, the chip body 41 has a sealing cover 414 thereon, and the sealing cover 414 is disposed around the notches of the oil phase reservoir 4111 and the water phase reservoir 4112, so that each of the oil phase reservoir 4111 and the water phase reservoir 4112 is sealed and independent from each other. Preferably, the micro-droplet generating part 412 includes a cross port 4121, and the cross port 4121 includes two oil phase ports corresponding to the oil phase reservoir 4111, a water phase port corresponding to the water phase reservoir 4112, and an output port corresponding to the collecting part 413, and the output port is communicated with the collecting part 413 through a channel (inside the chip body 41, which may be formed by gluing and thus has sealing property). Therefore, the generation process of the micro-droplets is ensured to be in a relatively closed space, the sample reagent generates the droplets from the liquid storage part 411 to the micro-droplet generation part 412, and then the sample reagent works in a closed environment to the collection part 413, so that the problems of easy volatilization, cross contamination and the like of the droplets are effectively avoided.
Further, still be provided with two support posts 103 on the base plate 100, two support posts 103 are in respectively the chip removes the both sides that the part 3 is on a parallel with its moving direction, air feed part 21 erects in two form the portal structure on the support post 103, work as when chip part 4 is in little liquid droplet and generates the position, air feed part 21 the gas outlet can with the pressurization mouth intercommunication that oil phase reservoir 4111 and water phase reservoir 4112 have respectively.
Preferably, the linear displacement driving component 31 includes a sliding rail 311, a motor fixing adapter 312, a lead screw fixing plate 313, a lead screw motor 314, and a photoelectric sensor 315, where the lead screw fixing plate 313 is fixedly connected to the substrate 100, a lead screw in the lead screw motor 314 is fixedly connected to the lead screw fixing plate 313, a motor housing in the lead screw motor 314 is fixedly connected to the sliding rail 311 through the motor fixing adapter 312, the sliding rail 311 is connected to the driven plate 32, and the photoelectric sensor 315 is configured to detect and feed back (to the control component 1) a real-time position of the lead screw motor 314. When the screw rod motor 314 receives a working signal, the screw rod is not moved at the moment, the motor performs horizontal linear motion, the corresponding motor fixing adapter 312 drives the sliding block on the sliding rail 311 to perform horizontal linear motion, and the sliding rail 311 ensures the linearity and stability of the motion in the motion process.
It is readily understood by a person skilled in the art that the advantageous ways described above can be freely combined, superimposed without conflict.
The present invention is not limited to the above preferred embodiments, and any modifications, equivalent substitutions and improvements made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims (10)
1. The utility model provides a little liquid droplet generates device, a serial communication port, includes base plate (100), fixedly connected with control unit (1), air feed unit (21) and atmospheric pressure regulating part (22) on base plate (100), air feed unit (21) include with base plate (100) fixed connection's gas cylinder (214), fixedly connected with air pump (212), snuffle valve (215) and can real-time supervision on gas cylinder (214) gas pressure's in gas cylinder (214) first pressure sensor (216), atmospheric pressure regulating part (22) are including the gas bomb that has a plurality of independent air cavities (222), gas cylinder (214) with air cavity (222) realize alternative intercommunication through air cavity intercommunication solenoid valve (223), every fixedly connected with second pressure sensor (221) and the parallelly connected oil phase solenoid valve (224) of mutual pipeline that are used for real-time detection corresponding air cavity (222) interior gas pressure on the chamber wall of air cavity (222) oil phase solenoid valve (224), The control component (1) is configured to be capable of controlling the air release valve (215) or the oil phase solenoid valve (224) and the water phase solenoid valve (225) to generate air release action according to the pressure values monitored by the first pressure sensor (216) and the second pressure sensor (221) in real time and when the pressure values exceed a preset pressure threshold range.
2. The microdroplet generation device according to claim 1, wherein the control component (1) is further configured to control the air pump (212) to operate or control the oil phase solenoid valve (224) and the water phase solenoid valve (225) to communicate the air cavity (222) with the chip component (4) downstream of the air channel when the pressure value is lower than a preset threshold range of pressure; or, the gas cylinder (214) is further provided with an air filter (211) and a one-way valve (213), wherein the air filter (211) is used for filtering the inlet gas of the gas pump (212), and the one-way valve (213) is used for preventing the reverse flow of the gas between the gas pump (212) and the gas cylinder (214).
3. The microdroplet generation device according to claim 1, further comprising a chip component (4) and a chip moving component (3), wherein the chip moving component (3) comprises a linear displacement driving component (31) and a driven plate (32), the chip component (4) is placed on the driven plate (32), and the linear displacement driving component (31) can drive the driven plate (32) to generate linear reciprocating motion.
4. The droplet generating apparatus according to claim 3, wherein the substrate (100) further comprises two displacement guide pillars (101), the two displacement guide pillars (101) are respectively disposed on two sides of the chip moving member (3) parallel to the moving direction thereof, the driven plate (32) and the two displacement guide pillars (101) are respectively provided with sliding grooves (102) on two corresponding sides, the displacement guide pillars (101) and the sliding grooves (102) are respectively provided with sliding blocks, and the sliding blocks are slidably inserted into the corresponding sliding grooves (102).
5. A microdroplet generation device as claimed in claim 4 wherein the chute (102) comprises a first horizontal guide section (1021), a second horizontal guide section (1022) and a transition section (1023) between the first horizontal guide section (1021) and the second horizontal guide section (1022) parallel to each other, the first horizontal guide section (1021) having a lower height than the second horizontal guide section (1022), the chip component (4) being in a microdroplet non-generation position when the slider is in the first horizontal guide section (1021) and the chip component (4) being in a microdroplet generation position when the slider is in the second horizontal guide section (1022).
6. A micro-droplet generating device according to claim 5, wherein the chip part (4) comprises a plurality of chip bodies (41) arranged side by side, the chip body (41) is provided with a liquid storage part (411), a micro-droplet generation part (412) and a collection part (413), wherein the liquid storage part (411) comprises an oil phase liquid storage tank (4111) and a water phase liquid storage tank (4112), the oil phase reservoir (4111) and the water phase reservoir (4112) are selectively communicated with the air cavity (222) through the oil phase electromagnetic valve (224) and the water phase electromagnetic valve (225) respectively, under the action of the pressure gas in the air cavity (222), the oil phase in the oil phase reservoir (4111) and the water phase in the water phase reservoir (4112) generate micro-droplets at the micro-droplet generating part (412) and are conveyed into the collecting part (413).
7. A droplet generating device according to claim 6, wherein the chip body (41) has a sealing cover (414), and the sealing cover (414) is correspondingly disposed around the oil phase reservoir (4111) and the water phase reservoir (4112) to seal each of the oil phase reservoir (4111) and the water phase reservoir (4112) independently from each other.
8. The microdroplet generation device according to claim 6, wherein the microdroplet generation part (412) comprises a cross port (4121), the cross port (4121) comprising two oil phase ports corresponding to the oil phase reservoir (4111), an aqueous phase port corresponding to the aqueous phase reservoir (4112), and an output port corresponding to the collection part (413), the output port communicating with the collection part (413) through a channel.
9. A droplet generating device according to claim 6, wherein two support columns (103) are further disposed on the substrate (100), two of the support columns (103) are respectively disposed on two sides of the chip moving part (3) parallel to the moving direction thereof, the gas supply part (21) is erected on the two support columns (103) to form a gantry structure, and when the chip part (4) is in the droplet generating position, the gas outlet of the gas supply part (21) can be communicated with the pressure ports of the oil phase reservoir (4111) and the water phase reservoir (4112).
10. The micro-droplet generation device according to claim 3, wherein the linear displacement driving component (31) comprises a slide rail (311), a motor fixing adapter (312), a lead screw fixing plate (313), a lead screw motor (314), and a photoelectric sensor (315), wherein the lead screw fixing plate (313) is fixedly connected with the substrate (100), a lead screw in the lead screw motor (314) is fixedly connected with the lead screw fixing plate (313), a motor housing in the lead screw motor (314) is fixedly connected with the slide rail (311) through the motor fixing adapter (312), the slide rail (311) is connected with the driven plate (32), and the photoelectric sensor (315) is used for detecting and feeding back a real-time position of the lead screw motor (314).
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| CN202010931507.4A CN112007705A (en) | 2020-09-07 | 2020-09-07 | Micro-droplet generating device |
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| CN202010931507.4A CN112007705A (en) | 2020-09-07 | 2020-09-07 | Micro-droplet generating device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114367320A (en) * | 2021-12-31 | 2022-04-19 | 广东永诺医疗科技有限公司 | Micro-droplet preparation device and preparation method thereof |
| CN114887674A (en) * | 2022-04-26 | 2022-08-12 | 北京百迈客生物科技有限公司 | Micro-droplet generating device |
| CN115254216A (en) * | 2022-07-21 | 2022-11-01 | 苏州索真生物技术有限公司 | Method for processing micro-fluidic chip |
-
2020
- 2020-09-07 CN CN202010931507.4A patent/CN112007705A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114367320A (en) * | 2021-12-31 | 2022-04-19 | 广东永诺医疗科技有限公司 | Micro-droplet preparation device and preparation method thereof |
| CN114887674A (en) * | 2022-04-26 | 2022-08-12 | 北京百迈客生物科技有限公司 | Micro-droplet generating device |
| CN114887674B (en) * | 2022-04-26 | 2024-03-08 | 北京百迈客生物科技有限公司 | Micro-droplet generation device |
| CN115254216A (en) * | 2022-07-21 | 2022-11-01 | 苏州索真生物技术有限公司 | Method for processing micro-fluidic chip |
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