CN114887674A - Micro-droplet generating device - Google Patents
Micro-droplet generating device Download PDFInfo
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- CN114887674A CN114887674A CN202210465986.4A CN202210465986A CN114887674A CN 114887674 A CN114887674 A CN 114887674A CN 202210465986 A CN202210465986 A CN 202210465986A CN 114887674 A CN114887674 A CN 114887674A
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- 238000001514 detection method Methods 0.000 claims abstract description 79
- 239000000463 material Substances 0.000 claims description 49
- 238000007789 sealing Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 12
- 238000004891 communication Methods 0.000 claims description 2
- 238000010586 diagram Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 239000000243 solution Substances 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000012530 fluid Substances 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 210000003437 trachea Anatomy 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- 230000000149 penetrating effect Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
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- 238000000926 separation method Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01L—MEASURING FORCE, STRESS, TORQUE, WORK, MECHANICAL POWER, MECHANICAL EFFICIENCY, OR FLUID PRESSURE
- G01L11/00—Measuring steady or quasi-steady pressure of a fluid or a fluent solid material by means not provided for in group G01L7/00 or G01L9/00
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/14—Means for pressure control
Abstract
The invention relates to the technical field of microfluidics, and provides a micro-droplet generation device, which comprises: the device comprises an air supply unit, an air pressure control unit, an air pressure detection unit and a micro-droplet generation device; the air pressure detection unit comprises an air channel plate, and an air shunting channel and a plurality of air pressure detection channels are arranged in the air channel plate; the air supply unit is connected with the inlet end of the air diversion channel, a plurality of outlet ends of the air diversion channel are connected with the inlet ends of the air pressure detection channels in a one-to-one correspondence mode through the air pressure control units, the air pressure control units are used for detecting the air pressure of the multi-path air output by the air diversion channel, and the outlet ends of the air pressure detection channels are connected with a plurality of air inlet interfaces of the micro-droplet generation device in a one-to-one correspondence mode. The micro-droplet generation equipment realizes gas diversion, pressure control and gas pressure detection through the gas circuit board and the gas pressure control unit, simplifies the structure of the micro-droplet generation equipment, reduces the volume and the weight of the micro-droplet generation equipment, and improves the portability of the micro-droplet generation equipment.
Description
Technical Field
The invention relates to the technical field of microfluidics, in particular to micro-droplet generation equipment.
Background
The micro-droplets are widely applied to the fields of medical analysis, medicine preparation and the like. At present, there are many techniques for generating micro-droplets, such as membrane emulsification, spray emulsification, microfluidic chip method, and liquid-discharging gun-head injection method. The microfluidic chip method is a microfluidic technology for precisely controlling and manipulating micro-scale fluids. The liquid drops generated by the method have high dispersibility and strong controllability, and have obvious superiority compared with other methods.
Among them, in the single cell sequencing technology, the preparation of single cell is a key step in single cell sequencing. Microfluidic technology is widely applied to the preparation of single-cell micro-droplets. The micro-droplet generation device is developed based on the microfluidic technology and can be used for completing the preparation of single-cell micro-droplets. The existing micro-droplet generation equipment for preparing single-cell micro-droplets is complex in operation, large in volume and weight and poor in portability.
Disclosure of Invention
The invention provides a micro-droplet generation device, which is used for solving the problems of complex operation, large volume and heavy weight of a single-cell micro-droplet generation device in the prior art.
The present invention provides a micro-droplet generating device comprising: the device comprises an air supply unit, an air pressure control unit, an air pressure detection unit and a micro-droplet generation device;
the air pressure detection unit comprises an air channel plate, and an air diversion channel and a plurality of air pressure detection channels are arranged in the air channel plate; the air feed unit with the entry end of gaseous reposition of redundant personnel passageway links to each other, a plurality of exit ends of gaseous reposition of redundant personnel passageway are with a plurality of the entry end of atmospheric pressure detection passageway passes through the atmospheric pressure control unit one-to-one links to each other, the atmospheric pressure control unit is used for detecting the gaseous atmospheric pressure of the multichannel of gaseous reposition of redundant personnel passageway output, it is a plurality of the exit end of atmospheric pressure detection passageway with a plurality of interfaces one-to-one that admit air of little liquid droplet generating device link to each other.
The micro-droplet generation equipment provided by the invention further comprises a shell, wherein the air supply unit, the air pressure control unit and the air pressure detection unit are arranged on the inner side of the shell; the micro-droplet generating device comprises a chip clamp, the chip clamp comprises a base and a cover body, the base is arranged on the shell, and the cover body is located on the outer side of the shell and covers the base.
According to the micro-droplet generating device provided by the invention, the micro-droplet generating device comprises a chip clamp and at least one micro-droplet chip, the chip clamp comprises a base and a cover body, the cover body is arranged on the base in a covering mode, the micro-droplet chip is clamped between the base and the cover body, and the air inlet interface is arranged on the cover body and communicated with the micro-droplet chip.
According to the micro-droplet generating apparatus provided by the invention, the chip clamp further comprises a plurality of guiding elements, the cover body is connected to the base through the plurality of guiding elements, and the cover body can move along the guiding elements to approach or move away from the base.
According to the droplet generating device provided by the invention, the base is respectively provided with slots corresponding to the positions of two ends of the droplet chip, two ends of the droplet chip are respectively and correspondingly inserted into the slots, at least one slot is internally provided with the elastic resetting piece, and the elastic resetting piece is tightly pressed between the end part of the droplet chip and the slot.
According to the micro-droplet generation equipment provided by the invention, a plurality of first air passages are arranged in the cover body, and the plurality of first air passages are communicated with the plurality of air inlet interfaces in a one-to-one correspondence manner; each micro-droplet chip is provided with at least one groove group structure, each groove group structure comprises a collecting groove and a plurality of material grooves, and the material grooves are respectively communicated with the collecting grooves through micro-channels;
each material groove is hermetically connected with the cover body to form a first accommodating cavity, and a plurality of first accommodating cavities corresponding to each groove group structure are communicated with a plurality of first air passages in a one-to-one correspondence manner.
According to the invention, the micro-droplet generating device also comprises a sealing member, wherein the sealing member is clamped between the micro-droplet chip and the cover body; the sealing element is provided with a plurality of first through holes which are respectively used for communicating the first air passage with the corresponding material grooves.
According to the droplet generation device provided by the invention, the air pressure control unit comprises a plurality of air pressure controllers, a plurality of outlet ends of the air diversion channels are communicated with the inlet ends of the plurality of air pressure controllers in a one-to-one corresponding pipeline manner, and the outlet ends of the plurality of air pressure controllers are communicated with the inlet ends of the plurality of air pressure detection channels in a one-to-one corresponding pipeline manner.
According to the micro-droplet generating device provided by the invention, the air supply unit comprises an air pump, and the outlet end of the air pump is connected with the inlet end of the air diversion channel.
According to the micro-droplet generating device provided by the invention, the micro-droplet generating device further comprises a display unit, the display unit is arranged on the outer side of the shell, and the air pressure control unit and the air pressure detection unit are respectively and electrically connected with the display unit.
The micro-droplet generation equipment provided by the invention is characterized in that the gas circuit board is arranged, the gas shunt channel and the plurality of air pressure detection channels are arranged in the gas circuit board, the air pressure control unit is arranged between the outlet end of the gas shunt channel and the inlet end of the air pressure detection channels, and the multi-path gas shunted by the gas circuit board flows back into the gas circuit board for air pressure detection after being subjected to air pressure regulation and control by the air pressure control unit, so that the gas circuit board integrates the functions of gas shunting and detection. The micro-droplet generation equipment can complete gas shunting, pressure control and gas pressure detection only through the gas circuit board and the gas pressure control unit, so that the structure of the micro-droplet generation equipment is simplified, the volume and the weight of the micro-droplet generation equipment are reduced, and the portability of the micro-droplet generation equipment is improved.
Drawings
In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings needed for the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and those skilled in the art can also obtain other drawings according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a micro-droplet generation apparatus provided by the present invention;
FIG. 2 is a schematic diagram of the droplet generation apparatus of FIG. 1 from another perspective;
fig. 3 is a schematic structural diagram of an air pressure detection unit in the micro-droplet generating device provided by the invention;
FIG. 4 is a schematic structural diagram of another view angle of a pressure detecting unit in the droplet generating apparatus provided by the present invention;
FIG. 5 is a schematic structural diagram of an air pressure controller in the micro-droplet generating device provided by the present invention;
fig. 6 is a schematic structural diagram of a micro-droplet generating device provided by the present invention when a cover is in a first position;
fig. 7 is a schematic structural view of a droplet generating device provided by the present invention with the cover in a second position;
FIG. 8 is a schematic diagram of a chip holder in a micro-droplet generating device according to the present invention;
FIG. 9 is a schematic view of the chip gripper of FIG. 8 from another perspective;
FIG. 10 is a schematic structural diagram of a droplet generating device of the present invention;
FIG. 11 is a schematic view of the micro-droplet chip of FIG. 10 from another perspective;
reference numerals are as follows:
1. a housing; 11. a mounting seat; 12. a mounting frame; 2. an air supply unit; 31. a pneumatic controller; 311. an air inlet; 312. an air outlet; 4. an air pressure detection unit; 41. a gas circuit board; 411. a gas distribution inlet; 42. an air pressure sensor; 43. a circuit board; 412a, a first branch gas outlet; 412b, a second gas separation outlet; 412c, a third gas distribution outlet; 413a, a first detection gas inlet; 413b, a second detection gas inlet; 413c, a third detection gas inlet; 414a, a first detection gas outlet; 414b, a second detection gas outlet; 414c, a third detection gas outlet; 5. a micro-droplet generating device; 51. a chip clamp; 511. a base; 5111. a trachea wiring hole; 5112. a slot; 5113. positioning the projection; 512. a cover body; 5121. a first air inlet interface; 513. a guide member; 52. a micro droplet chip; 521. collecting tank; 522a, a first material groove; 522b, a second material groove; 522c, a third material groove; 523. a plug; 6. a seal member; 61. a first through hole; 62. a second through hole; 63. positioning holes; 70. a main flow channel; 71. branch flow channels; 72. a second flow passage; 73. a third flow passage; 8. a display unit.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the embodiments of the present invention, it should be noted that the terms "first", "second" and "third" are used for the sake of clarity in describing the numbering of the components of the product and do not represent any substantial difference, unless explicitly stated or limited otherwise. Further, "a plurality" means two or more.
In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The micro-droplet generation device of the present invention is described below in conjunction with fig. 1-11.
As shown in fig. 1 and 2, a microdroplet generating device provided by an embodiment of the present invention includes: the device comprises an air supply unit 2, an air pressure control unit, an air pressure detection unit 4 and a micro-droplet generation device 5. The droplet generator 5 is provided with a plurality of air inlet ports. The air pressure detecting unit 4 includes an air channel plate 41, and an air flow dividing channel and a plurality of air pressure detecting channels are provided in the air channel plate 41. The gas supply unit 2 is connected with the inlet end of the gas distribution channel, and a plurality of outlet ends of the gas distribution channel are connected with the inlet ends of the plurality of gas pressure detection channels in a one-to-one correspondence mode through the gas pressure control units. The air pressure control unit is used for detecting the air pressure of the multi-path gas output by the gas diversion channels, and the outlet ends of the plurality of air pressure detection channels are connected with the plurality of air inlet interfaces in a one-to-one correspondence mode.
Specifically, as shown in fig. 3 and 4, the gas circuit board 41 is provided with a gas distribution inlet 411, a plurality of gas distribution outlets, a plurality of detection gas inlets and a plurality of detection gas outlets. The air outlet of the air supply unit 2 is communicated with the air distribution inlet 411. The plurality of gas distribution outlets are respectively communicated with the gas distribution inlet 411 to form a gas distribution passage penetrating the gas circuit board 41. The plurality of detection air inlets are communicated with the plurality of detection air outlets in a one-to-one correspondence manner to form a plurality of air pressure detection channels.
The air pressure detecting unit 4 further includes an air pressure sensor 42 for detecting the air pressure in the air pressure detecting channels. Each air pressure detecting channel is provided with an air pressure sensor 42. Optionally, the air pressure detecting unit further includes a circuit board 43, the circuit board 43 is disposed on one side of the air passage board 41, and the air pressure sensor 42 is located between the circuit board 43 and the air passage board 41 and electrically connected to the circuit board 43. The pneumatic control unit is provided with a plurality of air inlets and a plurality of air outlets, a pressure controller is arranged in the pneumatic control unit, the air inlets and the air outlets are connected in a one-to-one correspondence mode through the pressure controller, and adjustment and control of multi-path gas pressure are achieved.
The air pressure control unit is provided with a plurality of air inlet ports, a plurality of air outlet ports, a plurality of detection air inlet ports, a plurality of air outlet ports, a plurality of air distribution outlet ports, a plurality of detection air inlet ports, a plurality of detection air outlet ports and a plurality of air inlet port one-to-one corresponding pipelines.
When the micro-droplet generating apparatus is used for preparing micro-droplets, the gas output by the gas supply unit 2 enters the gas distribution plate 41 from the gas distribution inlet 411, and is distributed after passing through the gas distribution channels in the gas distribution plate 41 to form multiple paths of gas which respectively flow out from the multiple gas distribution outlets. The multi-path gas flowing out from the plurality of gas distribution outlets flows back into the gas path plate 41 from the plurality of detection gas inlets after passing through the gas pressure control unit, and then flows out from the plurality of detection gas outlets after passing through the plurality of gas pressure detection channels in the gas path plate 41. The multi-path gas flowing out from the plurality of detection gas outlets is respectively introduced into the micro-droplet generating device 5 from the plurality of gas inlet interfaces to form micro-droplets.
In the droplet generation apparatus provided in the embodiment of the present invention, by providing the gas channel plate 41, the gas diversion channel and the plurality of gas pressure detection channels are provided in the gas channel plate 41, and the gas pressure control unit is provided between the outlet end of the gas diversion channel and the inlet end of the gas pressure detection channels, so that the multiple paths of gas shunted by the gas channel plate 41 flow back into the gas channel plate 41 for gas pressure detection after being subjected to gas pressure adjustment and control by the gas pressure control unit, and the gas channel plate 41 integrates functions of gas diversion and gas detection. The micro-droplet generating equipment can complete gas shunting, pressure control and gas pressure detection only through the gas circuit board 41 and the gas pressure control unit, so that the structure of the micro-droplet generating equipment is simplified, the volume and the weight of the micro-droplet generating equipment are reduced, and the portability of the micro-droplet generating equipment is improved.
As a specific embodiment, the gas circuit board 41 is provided with three gas distribution outlets, three detection gas inlets and three detection gas outlets. A first branch gas outlet 412a, a second branch gas outlet 412b, a third branch gas outlet 412c, a first detection gas inlet 413a, a second detection gas inlet 413b, a third detection gas inlet 413c, a first detection gas outlet 414a, a second detection gas outlet 414b, and a third detection gas outlet 414c, respectively. Thereby forming three paths of gas, and the air pressure of the three paths of gas can be independently adjusted and controlled.
As shown in fig. 8 and 9, the droplet generating apparatus according to some embodiments of the present invention further includes a mounting base 11, and the gas supply unit 2 is mounted on the mounting base 11. The micro-droplet generator 5 includes a chip holder 51, and the chip holder 51 is mounted on the mounting base 11 via a mounting frame 12. An accommodating space is formed by enclosing the mounting frame 12, the chip clamp 51 and the mounting seat 11, and the accommodating space can be used for accommodating the air channel plate 41, the air pressure control unit and the air pressure detection unit 4, so that the whole equipment is compact in structure, and the chip clamp 51 is convenient to mount and dismount.
As shown in fig. 6 and 7, the micro-droplet generating apparatus according to some embodiments of the present invention further includes a housing 1, and the air supply unit 2, the air pressure control unit, and the air pressure detection unit 4 are disposed inside the housing 1. The mounting seat 11 in the above embodiments may be the bottom of the housing 1. The chip holder 51 includes a base 511 and a cover 512. The base 511 is provided on the housing 1, and the lid 512 is provided outside the housing 1 and covers the base 511.
The chip holder 51 is used for holding a droplet chip, and the droplet chip is disposed between the base 511 and the lid 512. The housing 1 is provided with an installation opening, the base 511 is provided at the installation opening, and the cover 512 is provided on the side of the base 511 facing the outside of the housing 1.
The air supply unit 2, the air pressure control unit and the air pressure detection unit 4 are all contained in the shell 1, and the integrity of the micro-droplet generation equipment is improved. The cover 512 has a first position and a second position. As shown in fig. 6, when the cover 512 is in the first position, the cover 512 covers the base 511, so as to clamp the droplet chip. At this moment, the micro-droplet generating device is integrated in the shell 1 and is easy to carry. As shown in fig. 7, when the cover 512 is at the second position, the cover 512 is separated from the base 511, so that the micro droplet chip can be taken.
In some embodiments of the present invention, the droplet generating apparatus 5 includes a chip holder 51 and at least one droplet chip 52, the chip holder 51 includes a base 511 and a cover 512, and the cover 512 covers the base 511. The droplet chip 52 is clamped between the base 511 and the cover 512. The air inlet of the droplet generator 5 is disposed on the cover 512 and communicates with the droplet chip 52.
The cover 512 is movably connected to the base 511 through a connecting member, for example, the cover 512 is rotatably connected to the base 511 through a rotating shaft. Alternatively, the cover 512 and the base 511 are two separable parts, and the cover 512 is detachably connected to the base 511.
One side of lid 512 towards base 511 is equipped with a plurality of interfaces that admit air, is equipped with a plurality of trachea on the base 511 and walks line hole 5111, and a plurality of interfaces that admit air and a plurality of trachea walk line hole 5111 one-to-one setting. The air pipes connecting the plurality of detection air outlets and the plurality of air inlet ports respectively penetrate through the corresponding air pipe wiring holes 5111.
In some embodiments of the present invention, the chip holder 51 further comprises a plurality of guiding members 513, the cover 512 is connected to the base 511 by the plurality of guiding members 513, and the cover 512 can move along the guiding members 513 to approach or separate from the base 511.
Specifically, the base 511 is provided with a plurality of guide holes, and the plurality of guide members 513 are inserted into the plurality of guide holes in a one-to-one correspondence. Optionally, one end of the guide 513 is fixedly connected to the cover 512, and the other end of the guide 513 is movably inserted into the guide hole. When it is desired to open the cover 512, the cover 512 is moved along the guide 513 in a direction away from the base 511. Alternatively, one guide 513 is provided corresponding to both sides of the cover 512, respectively.
Furthermore, a plurality of mounting holes are formed in the cover 512, a plurality of threaded holes are formed in the base 511, and the plurality of threaded holes and the plurality of mounting holes are arranged in a one-to-one correspondence manner. The cover 512 and the base 511 are connected by a screw member penetrating the mounting hole and the threaded hole, so that the cover 512 and the base 511 are clamped.
In some embodiments of the invention, as shown in fig. 8, a positioning groove is formed on the base 511, and the droplet chip 52 is positioned in the positioning groove. Specifically, the base 511 is provided with slots 5112 corresponding to two ends of the droplet chip 52, two ends of the droplet chip 52 are inserted into the slots 5112, and at least one of the slots 5112 is provided with an elastic resetting member, which is pressed between the end of the droplet chip 52 and the slot 5112.
Wherein, two ends of the micro droplet chip 52 are respectively provided with a plug 523. Alternatively, the slot 5112 is configured as a U-shaped slot, and correspondingly, the plug 523 is also configured as a U-shaped structure. The plug 523 is in clearance fit with the slot 5112, so that the micro droplet chip 52 is limited in the length direction and the width direction of the slot 5112. In the depth direction of the slot 5112, the micro droplet chip 52 is limited by the elastic restoring member.
Optionally, an elastic restoring element is disposed in the slot 5112 corresponding to one end of the droplet chip 52, and the droplet chip 52 is compressed between the elastic restoring element and another slot 5112. Optionally, two slots 5112 corresponding to two ends of the droplet chip 52 are respectively provided with an elastic reset member, and the droplet chip 52 is compressed between the two elastic reset members.
In some embodiments of the present invention, a plurality of first air passages are disposed in the cover 512, and the plurality of first air passages are in one-to-one correspondence with the plurality of air inlet interfaces. Each micro droplet chip 52 is provided with at least one groove group structure. The tank group structure comprises a collecting tank 521 and a plurality of material tanks which are respectively communicated with the collecting tank 521 through micro-channels. Each material tank is hermetically connected with the cover body 512 to form a first accommodating cavity, and a plurality of first accommodating cavities corresponding to each tank group structure are communicated with a plurality of first air passages in a one-to-one correspondence manner.
Under the condition that the cover body 512 covers the base 511, the notches of the material grooves are respectively connected with the cover body 512 in a sealing manner to form a first accommodating cavity, and the first accommodating cavity is used for accommodating liquid. The first accommodating cavities formed between the material grooves of each groove group structure and the cover body 512 are respectively used for accommodating different liquids and are communicated with the first air passages in a one-to-one correspondence manner. The cover 512 is provided with a plurality of first air inlet interfaces 5121, the plurality of first air passages are correspondingly connected to the plurality of first air inlet interfaces 5121, and each first air inlet interface 5121 is connected to a detection air outlet of the air pressure detection unit 4.
When the micro-droplet generation equipment is used, gas output by the detection gas outlets is correspondingly introduced into the first gas passages, and then enters the corresponding material grooves from the gas outlets of the first gas passages. Different air pressures can be respectively applied to the liquid in the material grooves through the first air passages. The liquid in the material tanks is converged into the collecting tank 521 through the micro-channels on the micro-droplet chip 52 under the action of pressure to form micro-droplets.
As shown in fig. 8 and 10, in the present embodiment, the number of the micro droplet chips 52 is four, the number of the first air passages is three, each micro droplet chip 52 is provided with two groove sets, each groove set includes three material grooves, which are a first material groove 522a, a second material groove 522b, and a third material groove 522 c. One of the first gas passages is used for simultaneously introducing pressure gas into the first material grooves 522a of the four micro droplet chips 52; another first gas channel is used for simultaneously introducing the pressure gas into the second material channel 522b of the four micro droplet chips 52; yet another first gas channel is used to simultaneously introduce pressurized gas into third material channels 522c of four droplet chips 52.
The first material groove 522a, the second material groove 522b, the third material groove 522c and the collecting groove 521 are arranged along the length direction of the micro droplet chip 52. Alternatively, in the case where the micro droplet chip 52 is provided with a plurality of groove group structures, the plurality of groove group structures are arranged along the width direction of the micro droplet chip 52.
In this embodiment, the micro droplet chips 52 are disposed between the cover 512 and the base 511, and the cover 512 is provided with a plurality of first air channels, so that the material slots of each slot set structure are in one-to-one correspondence with the first air channels, and each first air channel can apply pressure to one material slot of each of the micro droplet chips 52. When the micro droplet chip 52 is clamped, a plurality of micro droplet chips 52 are placed between the base 511 and the cover 512, and the material groove opening of the micro droplet chip 52 is communicated with the air outlet of the corresponding first air passage of the cover 512. The material tank with the structure of the plurality of tank groups of the plurality of micro-droplet chips 52 can be supplied with gas at the same time, the batch preparation of a plurality of groups of micro-droplets is realized, and the operation is simple and rapid.
As shown in fig. 11, the micro flow channel includes a primary flow channel 70, a first flow channel, a second flow channel 72, and a third flow channel 73. One end of the main flow passage 70 is connected to the collecting tank 521, and the other end is connected to one ends of the second flow passage 72 and the third flow passage 73. The other end of the second flow path 72 and the other end of the third flow path 73 are connected to the second material tank 522b and the third material tank 522c, respectively. The first flow channel includes two branch flow channels 71, the two branch flow channels 71 are respectively connected to the first material groove 522a and the main flow channel 70, and the two branch flow channels 71 are located at two sides of the main flow channel 70.
In the process of generating single-cell micro-droplets, the first material tank 522a is used for containing the second phase liquid. The cell sap in the third material tank 522c flows into the third channel 73 under pressure to form a single cell, the second phase fluid in the second material tank 522b flows into the second channel 72 under pressure, and the second phase fluid and the cell sap are mixed in the main channel 70. The first-phase liquid in the first material tank 522a is divided into two branch flow channels 71 under the action of pressure, then enters the main flow channel 70 from two sides of the main flow channel 70, and is mixed and wrapped with the mixed liquid in the main flow channel 70 to form single-cell micro-droplets.
Further, the cover 512 is further provided with a second air duct (not shown) and a second air inlet (not shown). The collecting tank 521 is hermetically connected to the cover 512 to form a second accommodating cavity, and the second accommodating cavity is correspondingly communicated with the second air passage. The second air passage is communicated with a second air inlet interface, and the second air inlet interface is connected with a detection air outlet of the air pressure detection unit 4.
The total number of the first air inlet interfaces and the second air inlet interfaces is the same as the total number of the detection air outlets of the air pressure detection unit 4, and the first air inlet interfaces and the second air inlet interfaces are communicated with one another through corresponding pipelines. Under the condition that the cover body 512 covers the base 511, the notch of the collecting tank 521 is hermetically connected with the cover body 512 to form a second accommodating cavity, and the second accommodating cavity is used for accommodating mixed liquid converged from a plurality of material tanks.
When the micro-droplet generating device is used, gas output from a detection gas outlet is introduced into the second gas channel, and then enters the collecting tank 521 from a gas outlet of the second gas channel, so as to apply pressure to liquid in the collecting tank 521. Through the two-way regulation of the air pressure in the collecting groove 521 and the material groove, the accurate control of the air pressure in the material groove can be realized.
Some embodiments of the present invention provide a micro-droplet generating device further comprising a sealing member 6, wherein the sealing member 6 is clamped between the micro-droplet chip 52 and the cover 512. A plurality of first through holes 61 are formed in the sealing element 6, and the first through holes 61 are respectively used for communicating the first air channel with the corresponding material grooves. Namely, the material grooves of each groove group structure are communicated with the first air passages through the first through holes 61 in a one-to-one correspondence manner.
Further, in the case that the cover 512 is further provided with a second air passage, the sealing member 6 further includes a plurality of second through holes 62, and the plurality of second through holes 62 are respectively used for communicating the second air passage with the plurality of collecting grooves 521. That is, each of the collection grooves 521 communicates with the second gas passages through a separate one of the second through holes 62.
On the basis of the above-described embodiment, 24 first through holes 61 and 8 second through holes 62 are provided in the sealing member 6. The first air channel is correspondingly communicated with 24 material grooves on the four micro-droplet chips 52 through 16 first through holes 61. The second air passages are correspondingly communicated with 8 collecting grooves 521 on the four micro-droplet chips 52 through 8 second through holes 62.
Furthermore, a positioning protrusion 5113 is disposed on a side of the base 511, and the sealing member 6 is provided with a positioning hole 63 adapted to the positioning protrusion 5113. Optionally, the seal 6 is a sealing gasket. When the sealing gasket is installed, the positioning hole 63 is matched with the positioning protrusion 5113 to realize quick installation and positioning, so that the first through hole 61 is communicated with the corresponding material groove, and the second through hole 62 is communicated with the corresponding collecting groove 521.
In some embodiments of the invention, the first airway includes a main airway and a plurality of branch airways connected in parallel to the corresponding main airway. And the plurality of branch air passages of the first air passage are communicated with the corresponding plurality of material grooves in a one-to-one correspondence manner.
Specifically, as shown in fig. 9, a plurality of air holes are formed on a side of the cover 512 facing the droplet chip 52, and each air branch is correspondingly communicated with one air hole. The number of the micro droplet chips 52 is n, the number of the groove group structures on each micro droplet chip 52 is m, and the number of the branch air passages of each first air passage is the product of n and m.
For example, in the above embodiment, four micro droplet chips 52 have eight first material channels 522a, and one of the first air channels includes eight branch air channels. One ends of the eight branch air ducts are respectively communicated with the main air duct, and under the condition that the cover body 512 is covered on the base 511, the other ends of the eight branch air ducts are communicated with the eight first material grooves 522a in a one-to-one correspondence manner.
Optionally, the second air passage has the same structure as the first air passage, and also includes a main air passage and a plurality of branch air passages, and the plurality of branch air passages are connected in parallel to the corresponding main air passage. The plurality of branch air passages of the second air passage are communicated with the corresponding plurality of collecting tanks 521 in a one-to-one correspondence manner. The number of branch airway of the second airway is the same as the number of branch airway of the first airway.
In some embodiments of the present invention, the air pressure control unit comprises a plurality of air pressure controllers 31, as shown in fig. 5, each air pressure controller 31 is provided with an air inlet 311 and an air outlet 312. The outlet ends of the gas diversion channels of the gas circuit board 41 are in one-to-one correspondence pipe communication with the gas inlets 311 of the plurality of gas pressure controllers 31. The gas output from the gas distribution outlet of the gas path plate 41 enters from the gas inlet 311 of the gas pressure controller 31, is output from the gas outlet 312 after the gas pressure is adjusted by the internal pressure controller, and then flows back to the gas pressure detection channel in the gas path plate 41. The air outlets 312 of the air pressure controllers 31 are in one-to-one correspondence with the inlet ends of the air pressure detection channels. By providing a plurality of air pressure controllers 31, the mounting positions of the plurality of air pressure controllers 31 in the housing 1 can be flexibly set, which is advantageous for the miniaturization of the micro-droplet generating apparatus.
In some embodiments of the present invention, the air supply unit 2 comprises an air pump, and an outlet end of the air pump is connected to an inlet end of the air diversion channel. That is, the outlet of the air pump is connected to the air dividing inlet 411 of the air channel plate 41, and the inlet of the air pump is connected to the air supply device or the air storage device.
The micro-droplet generating device provided by some embodiments of the present invention further includes a display unit 8, the display unit 8 is disposed outside the housing 1, and the air pressure control unit and the air pressure detection unit 4 are electrically connected to the display unit 8, respectively. The display unit 8 can be used to display the current system working state, such as the multi-channel gas pressure output by the gas pressure control unit and the multi-channel gas pressure detected by the gas pressure detection unit 4. Optionally, the display unit 8 is provided with an input module, and the air pressure control parameters of the air pressure control unit can be input through the input module.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. A micro-droplet generation apparatus, comprising: the device comprises an air supply unit, an air pressure control unit, an air pressure detection unit and a micro-droplet generation device;
the air pressure detection unit comprises an air channel plate, and an air distribution channel and a plurality of air pressure detection channels are arranged in the air channel plate; the air feed unit with the entry end of gaseous reposition of redundant personnel passageway links to each other, a plurality of exit ends of gaseous reposition of redundant personnel passageway are with a plurality of the entry end of atmospheric pressure detection passageway passes through the atmospheric pressure control unit one-to-one links to each other, the atmospheric pressure control unit is used for detecting the gaseous atmospheric pressure of the multichannel of gaseous reposition of redundant personnel passageway output, it is a plurality of the exit end of atmospheric pressure detection passageway with a plurality of interfaces one-to-one that admit air of little liquid droplet generating device link to each other.
2. The micro-droplet generating apparatus according to claim 1, further comprising a housing, wherein the gas supply unit, the gas pressure control unit, and the gas pressure detection unit are provided inside the housing; the micro-droplet generating device comprises a chip clamp, the chip clamp comprises a base and a cover body, the base is arranged on the shell, and the cover body is located on the outer side of the shell and covers the base.
3. The droplet generating apparatus according to claim 1, wherein the droplet generating device comprises a chip holder and at least one droplet chip, the chip holder comprises a base and a cover, the cover is disposed on the base, the droplet chip is clamped between the base and the cover, and the air inlet is disposed on the cover and is in communication with the droplet chip.
4. The micro-droplet generation apparatus of claim 3, wherein the chip holder further comprises a plurality of guides, the cover being connected to the base by the plurality of guides, the cover being movable along the guides to move closer to or away from the base.
5. The droplet generating apparatus according to claim 3, wherein the base has slots corresponding to two ends of the droplet chip, the two ends of the droplet chip are respectively inserted into the slots, and an elastic restoring member is disposed in at least one of the slots and compressed between the end of the droplet chip and the slot.
6. The droplet generation device according to claim 3, wherein a plurality of first air passages are provided in the cover body, and the plurality of first air passages are communicated with the plurality of air inlet ports in a one-to-one correspondence manner; each micro-droplet chip is provided with at least one groove group structure, each groove group structure comprises a collecting groove and a plurality of material grooves, and the material grooves are respectively communicated with the collecting groove through micro-channels;
each material groove is connected with the cover body in a sealing mode to form a first containing cavity, and a plurality of first containing cavities corresponding to each groove group structure are communicated with a plurality of first air passages in a one-to-one correspondence mode.
7. The microdroplet generation device of claim 6, further comprising a seal clamped between the microdroplet chip and the cover; the sealing element is provided with a plurality of first through holes which are respectively used for communicating the first air passage with the corresponding material grooves.
8. The droplet generating apparatus according to claim 1, wherein the air pressure control unit comprises a plurality of air pressure controllers, a plurality of outlet ends of the air diversion channels are in one-to-one correspondence with inlet ends of the plurality of air pressure controllers, and outlet ends of the plurality of air pressure controllers are in one-to-one correspondence with inlet ends of the plurality of air pressure detection channels.
9. The micro-droplet generating apparatus according to claim 1, wherein the gas supply unit comprises a gas pump, and an outlet end of the gas pump is connected to an inlet end of the gas diversion channel.
10. The micro-droplet generating apparatus according to claim 2, further comprising a display unit disposed outside the housing, the air pressure control unit and the air pressure detection unit being electrically connected to the display unit, respectively.
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