CN109289949A - A kind of full-automatic microlayer model emulsion generating means and its generation method - Google Patents
A kind of full-automatic microlayer model emulsion generating means and its generation method Download PDFInfo
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- CN109289949A CN109289949A CN201811194968.7A CN201811194968A CN109289949A CN 109289949 A CN109289949 A CN 109289949A CN 201811194968 A CN201811194968 A CN 201811194968A CN 109289949 A CN109289949 A CN 109289949A
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- microlayer
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- 239000000839 emulsion Substances 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 24
- 238000007789 sealing Methods 0.000 claims abstract description 17
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000003825 pressing Methods 0.000 claims abstract description 8
- 230000015572 biosynthetic process Effects 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 6
- 230000005484 gravity Effects 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000005755 formation reaction Methods 0.000 abstract description 11
- 238000002360 preparation method Methods 0.000 abstract description 2
- 230000004888 barrier function Effects 0.000 abstract 1
- 239000006210 lotion Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000006071 cream Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000004519 grease Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 108020004414 DNA Proteins 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000004530 micro-emulsion Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
Abstract
The present invention provides a kind of full-automatic microlayer model emulsion generating means and generation methods, including pedestal and supporting plate, pedestal is equipped with sliding rail, microlayer model is provided with by chip fixture on sliding rail and generates chip, the side of chip fixture is connect with driving motor, and driving motor movement drives chip fixture to be moved on the slide rail；The side of supporting plate is equipped with guide rail, and guide rail is equipped with pressure control mould group, is additionally provided with a cylinder above guide rail, and the cylinder axis of cylinder is connect with the connecting plate, cylinder moving, and pressure control mould group is driven to be moved on the guide rail.The present invention is adjusted drop formation reaction condition by automatic configuration and meets different application demand to generate different size of microlayer model, has completely cut off sample pollution by sealing cover, protects microlayer model to generate chip and is not damaged in the process of running by pressing plate.Eight channel barrier systems realize the drop preparation in two channels or the channel of its multiple, more convenient compared to existing equipment, save amount of reagent.
Description
Technical field
The invention belongs to microfluidic art more particularly to a kind of full-automatic microlayer model emulsion generating means and its lifes
At method.
Background technique
The microlayer model lotion that grease mutually generates has been applied to high-throughput analysis system, and application range has continuous expansion
Trend.For example be used to combine with polymerase chain reaction (PCR) by lotion formation, the list of nucleic acid target molecule is carried out in drop
The amplification of copy, to realize to the presence of unimolecule target and the detection of its gene order.U s company 10X Genomics is answered
With the method for emulsion-based PCR and in conjunction with GemCode technology next life growth chain DNA, to realize the sequencing or slender of long-chain DNA sequence dna
Born of the same parents' sequencing is generally recognised as carrying out one of long-chain DNA sequencing and the best technique of unicellular sequencing by industry.It can be grease two
Mutually controllable in the ratio mixing generation size of design, uniform, it is to realize above-mentioned answer that the lotion of stable micro emulsion drop, which generates instrument,
Key equipment.It is single that microlayer model emulsion currently on the market generates instrument system function, or has complicated for operation, easy production
Raw sample pollution.
With the raising of technical requirements, especially biochemistry detection field, these equipment are difficult to meet its demand.Such as Bole
The lotion of Biorad forms system, and drop formation is by loading the negative pressure offer power in the hole that drop is collected, oil phase and sample
Product enter micro-fluidic runner from the hole of charge of oil and dress sample respectively under negative pressure and flow to the hole that drop is collected.Such system is direct
Receive the limitation of micro-fluidic flow passage resistance force of waterproof, the use ratio of oily phase reagent and sample is substantially stationary, generates the size of drop
It is substantially stationary.Meanwhile the lotion of Bole BioRad generates micro-fluidic chip and has 8 channels (can carry out the cream of 8 samples simultaneously
Liquid preparation), but their system must do 8 channels simultaneously, if user in the case where having less than 8 samples, Yong Hubi
Oil or the populated remaining channel of buffer must be caused the waste in the use of channel.If there is adding for a hole in 8 holes
Sample wrong volume, the drop formation that will cause 8 holes are simultaneously stopped, and cause the loss of sample.Furthermore in the instrument use process
Concrete reason can not be known by generating unsuccessfully situation, user for drop, can only be abandoned drop and be generated chip with the reagent used, weigh
New operation.
Summary of the invention
In order to solve the deficiencies in the prior art, the present invention provides a kind of full-automatic microlayer model emulsion generating means and its
Generation method.
The purpose of the present invention is achieved through the following technical solutions:
A kind of full-automatic microlayer model emulsion generating means, including pedestal, are vertically installed in the supporting plate of pedestal, set on the pedestal
It is equipped with a sliding rail, microlayer model is provided on the sliding rail and generates chip, the microlayer model generates chip and is arranged by chip fixture
In on the sliding rail, the side of the chip fixture is connect with driving motor, and the driving motor movement drives the chip gripper
Tool is horizontally moved on the sliding rail；The side of the supporting plate is provided with guide rail, passes through connecting plate on the guide rail
It is provided with pressure control mould group, a cylinder is additionally provided with above the guide rail, the cylinder axis of the cylinder and the connecting plate connect
It connects, the cylinder moving, the pressure control mould group is driven to be moved above and below on the guide rail.
Preferably, it includes 8 groups of microlayer model generation groups that the microlayer model, which generates chip, every group of microlayer model generation group with by the
The oilhole of one pressure source connection, the sample well being connect with second pressure source and the drop collection hole composition with atmosphere, oilhole,
Sample well and drop collect hole by runner connection, and every two groups of microlayer model generation groups are controlled by same control valve.
Preferably, two oilholes in every two groups of microlayer models generation group share same Air Valve Control, and every two groups of microlayer models are raw
Two sample wells in groups share same pressure valve control.
Preferably, the air valve and pressure valve are connect with the air pressure control module telecommunications.
Preferably, sealing cover is additionally provided on the microlayer model chip, the chip fixture passes through pallet and driving motor
Connection.
Preferably, the guide rail is connect by connecting plate with the supporting plate.
Preferably, the driving motor is connect by connecting rod with the chip fixture, one end setting of the driving motor
In on the supporting plate.
Preferably, described device further includes a shell, and touch screen is provided on the shell, offers and adds on the shell
Window is carried, the pallet is back and forth shunk in the load window, and the touch screen and industrial PC are electrically connected, the air pressure
Control module and the industrial PC are electrically connected.
Preferably, a kind of full-automatic microlayer model emulsion generation method described in any of the above, includes the following steps,
S1, sample and the load of drop formation oil phase reagent；
S11, oily phase sample and aqueous sample are loaded respectively in the oilhole and sample well that microlayer model generates chip；
S12, by chip sealing be placed on microlayer model generate chip on be installed in place, guarantee microlayer model generate chip on each hole
Position is all by sealed envelope；
S2, chip load；
S21, ready microlayer model chip is fixed on chip fixture；
S22, driving motor movement, drive chip fixture to move to required position on the slide rail；
The generation of S3, microlayer model:
S31, cylinder drive pressure control mould group mobile to chip fixture direction, up to pressing chip sealing cover；
S32, pressure control mould group start to start work, and the high-precision gas pressure that pressure control mould group generates to microlayer model generates
Chip sample hole and oilhole, aqueous sample and oily phase reagent enter the microlayer model that chip interior generates stable uniform by fluid channel
Emulsion；
S33, generation microlayer model emulsion pass through fluid channel flow into chip drop collect hole；
After the completion of S34, drop generate, air pressure module from service, microlayer model generation chip inlet gas pressure is unloaded to and atmospheric pressure
It is identical；
S4, microlayer model collect transfer:
After S41, microlayer model generate, cylinder driving pressure control mould group is moved upwards, and driving motor drives pallet to overhanging
Out, microlayer model chip and fixture are taken out, pallet is withdrawn and completes entire process by driving motor.
Preferably, negative by being loaded in chip oil phase reagent entrance in the S34 when oily phase reagent specific gravity is higher than drop
Pressure, by the oily phase reagent suction part of drop collection hole bottom to oily phase reagent entrance.
The beneficial effects of the present invention are embodied in: drop formation reaction condition is adjusted by automatic configuration, to generate
Different size of microlayer model meets a variety of different application demands, realizes the drop system in two channels or the channel of its multiple
It is standby, compared with the prior art in once can only simultaneously 8 channel drop formation instruments it is more convenient, while saving using amount of reagent,
Isolation sample pollution is designed by sealing cover, protects microlayer model to generate chip and is not damaged in the process of running by pressing plate.
Detailed description of the invention
Fig. 1: schematic perspective view of the invention.
Fig. 2: Fig. 1 main view, has concealed connecting plate at this time.
Fig. 3: microlayer model generates chip structure schematic diagram.
Fig. 4: the principle assumption diagram of microlayer model chip.
Fig. 5: system control module connection schematic diagram of the invention.
Specific embodiment
It is specifically described technical solution of the present invention with reference to embodiments, present invention discloses a kind of full-automatic microlayer model creams
Turbid generating means and its generation method.In conjunction with shown in Fig. 1-Fig. 4, the present apparatus includes pedestal 2, is vertically installed in the supporting plate of pedestal
3, a sliding rail 6 is provided on the pedestal 2, and microlayer model is provided on the sliding rail 6 and generates chip.Specifically, on the sliding rail 6
It is provided with pallet 8, the microlayer model generates chip 83 and is set on the pallet 8 by chip fixture 81, and the one of the pallet 8
Side is connect with driving motor 82.It is enterprising in the sliding rail 6 that the movement of driving motor 82 drives the microlayer model to generate chip 83
Row horizontally moves.The driving motor 82 is connect by connecting rod with the chip fixture, one end of the driving motor 82
It is set on the supporting plate 3.Certainly, the driving motor 82 can also be fixed on pedestal 2, for realizing its work meaning not
The position of influence is with no restriction.
The side of the supporting plate 3 is provided with guide rail, and connecting plate is provided with pressure control mould group 5, institute on the guide rail
It states and is additionally provided with a cylinder 4 above guide rail, the cylinder axis of the cylinder 4 is connect with the connecting plate, the cylinder moving, is driven
The pressure control mould group 5 is moved above and below on the guide rail.The guide rail is connect by connecting plate 7 with the supporting plate 3.
The pressure control mould group bottom is provided with an air pressure pressing plate, and when work, the air pressure pressing plate will be pressed with chip sealing cover
Close contact.
A shell (not illustrating in figure) is additionally provided on the pedestal of the present apparatus, for the reality of preferably cooperation integral system
It applies, touch screen is provided on shell of the present invention, the touch screen and industrial PC are electrically connected, the air pressure control module
It is electrically connected with the industrial PC.In order to preferably be monitored, described device is additionally provided with the report connecting with industrial PC
Alert component.If operational process Instrumental detects mistake, user software real-time display false alarm stops instrument operation.It can
The loss of sample is reduced or avoided.Load window is offered on the shell, the driving motor 82 drives the pallet in institute
Stating load, window is inside and outside moves back and forth.The pallet reciprocates through the Mobile Slide control module connecting with industrial PC
It is controlled, is provided between the inside 1 and the supporting plate 3 of the shell for cooling fan.Movement of the present invention
Slide unit control module also controls the movement of the pressure control mould group.
It includes 8 groups of microlayer model generation groups that the microlayer model, which generates chip, every group of microlayer model generation group with by first pressure
The oilhole of source connection, the sample well being connect with second pressure source and the drop collection hole composition with atmosphere, oilhole, sample well
Hole is collected by runner connection with drop, and every two groups of microlayer model generation groups are controlled by same control valve.Specifically, knot
It closes the oilhole shown in Fig. 4, in two groups of adjacent microlayer model generation groups to connect by air valve, in two adjacent groups microlayer model generation group
Sample well is connected by same pressure valve, meanwhile, air valve is attached with corresponding pressure source with pressure valve.The air valve and
Pressure valve is connect with the air pressure control module telecommunications.Through the above structure, it is combined using different air pressures, applies different pressures
Power is oily mutually with flow velocity of the sample in the pipeline of miniflow hole to adjust drop formation reaction condition to adjust, and not only can control
Drop collects the volume ratio of oil and drop in hole, and can produce different size of microlayer model, meets a variety of differences and answers
With occasion demand.
8 channels are classified as 4 groups by the present invention, every group by air valve and pressure valve control respectively it is oily mutually and the pressure of sample, this
A little valves can individually control its switch.When user has less than 8 samples, user need to only open the valve of needs to generate
Drop will not generate waste to unused channel.For example, user, there are two sample, user only loads two on micro-fluidic chip
The sample and oil of a channel (channel 1 and 2) open valve A and valve B in the operation for generating drop.The channel 3- of micro-fluidic chip
It can also be used after 8.In specific implementation, control can also be implemented separately to each sample path in device, in this way can be into one
Step improves reagent service efficiency.
It in order to guarantee that microlayer model generates not by outside contamination, while being also the effective leakproofness for increasing pneumatic structure,
It is additionally provided with sealing cover 87 on the microlayer model chip, is offered on sealing cover 87 and generates chip oilhole, sample well with microlayer model
And drop collects the corresponding oilhole sealing cover 84 in hole, sample well sealing cover 85 and drop and collects hole sealing cover 86.
Present invention further teaches it is a kind of by full-automatic microlayer model emulsion process units carry out microlayer model generation method,
Include the following steps,
S1, sample and the load of drop formation oil phase reagent；
S11, oily phase sample and aqueous sample are loaded by pipettor respectively in the oilhole and sample well that microlayer model generates chip；
S12, by chip sealing be placed on microlayer model generate chip on be installed in place, guarantee microlayer model generate chip on each hole
Position is all by sealed envelope；
S2, chip load；
S21, ready microlayer model chip is fixed on chip fixture；
S22, driving motor movement, drive chip fixture to move to required position on the slide rail；
The generation of S3, microlayer model:
S31, cylinder drive pressure control mould group mobile to chip fixture direction, up to pressing chip sealing cover；
S32, pressure control mould group start to start work, and the high-precision gas pressure that pressure control mould group generates to microlayer model generates
Chip sample hole and oilhole, aqueous sample and oily phase reagent enter the microlayer model that chip interior generates stable uniform by fluid channel
Emulsion；
S33, generation microlayer model emulsion pass through fluid channel flow into chip drop collect hole；
After the completion of S34, drop generate, air pressure module from service, microlayer model generation chip inlet gas pressure is unloaded to and atmospheric pressure
It is identical；When oily phase reagent specific gravity is higher than drop, by loading negative pressure in chip oil phase reagent entrance, drop is collected into bottom hole
The oily phase reagent suction part in portion is to oily phase reagent entrance.
S4, microlayer model collect transfer:
After S41, microlayer model generate, cylinder driving pressure control mould group is moved upwards, and driving motor drives pallet to overhanging
Out, microlayer model chip and fixture are taken out, pallet is withdrawn and completes entire process by driving motor.
The beneficial effects of the present invention are embodied in: drop formation reaction condition is adjusted by automatic configuration, to generate
Different size of microlayer model meets a variety of different application demands, realizes the drop system in two channels or the channel of its multiple
It is standby, compared with the prior art in once can only simultaneously 8 channel drop formation instruments it is more convenient, while saving using amount of reagent,
Isolation sample pollution is designed by sealing cover, protects microlayer model to generate chip and is not damaged in the process of running by pressing plate.
Certainly still there are many specific embodiments by the present invention, are just not listed one by one herein.It is all using equivalent replacement or
Equivalent transformation and all technical solutions formed, all fall within the scope of protection of present invention.
Claims (10)
1. a kind of full-automatic microlayer model emulsion generating means, it is characterised in that: including pedestal, be vertically installed in the branch of pedestal
Plate is provided with a sliding rail on the pedestal, and microlayer model is provided on the sliding rail and generates chip, it is logical that the microlayer model generates chip
It crosses chip fixture to be set on the sliding rail, the side of the chip fixture is connect with driving motor, the driving motor movement
The chip fixture is driven horizontally to be moved on the sliding rail；The side of the supporting plate is provided with guide rail, described to lead
Pressure control mould group is provided with by connecting plate on rail, is additionally provided with a cylinder, the cylinder axis of the cylinder above the guide rail
It is connect with the connecting plate, the cylinder moving, the pressure control mould group is driven to be moved above and below on the guide rail.
2. a kind of full-automatic microlayer model emulsion generating means as described in claim 1, it is characterised in that: the microlayer model generates
Chip includes 8 groups of microlayer model generation groups, the oilhole that every group of microlayer model generation group is connect with by first pressure source, with second pressure
The sample well and collect hole composition with the drop of atmosphere that source connects, oilhole, sample well and drop collect hole and pass through runner connection
It is logical, and every two groups of microlayer model generation groups are controlled by same control valve.
3. a kind of full-automatic microlayer model emulsion generating means as claimed in claim 2, it is characterised in that: every two groups of microlayer models are raw
Two oilholes in groups share same Air Valve Control, and two sample wells in every two groups of microlayer models generation group share same pressure
Valve control.
4. a kind of full-automatic microlayer model emulsion generating means as claimed in claim 3, it is characterised in that: the air valve and pressure
Valve and the air pressure control module are electrically connected.
5. a kind of full-automatic microlayer model emulsion generating means as claimed in claim 3, it is characterised in that: the microlayer model core
On piece is additionally provided with sealing cover, and the chip fixture is connect by pallet with driving motor.
6. a kind of full-automatic microlayer model emulsion generating means as described in claim 1, it is characterised in that: the guide rail passes through
Connecting plate is connect with the supporting plate.
7. a kind of full-automatic microlayer model emulsion generating means as described in claim 1, it is characterised in that: the driving motor
It is connect by connecting rod with the chip fixture, one end of the driving motor is set on the supporting plate.
8. a kind of full-automatic microlayer model emulsion generating means as claimed in claim 3, it is characterised in that: described device further includes
One shell is provided with touch screen on the shell, offers load window on the shell, the pallet in the load window into
Row is reciprocal to be shunk, and the touch screen and industrial PC are electrically connected, and the air pressure control module electrically connects with the industrial PC
It connects.
9. a kind of full-automatic microlayer model emulsion production method as described in claim 1-8 is any, it is characterised in that: including such as
Lower step,
S1, sample and the load of drop formation oil phase reagent；
S11, oily phase sample and aqueous sample are loaded respectively in the oilhole and sample well that microlayer model generates chip；
S12, by chip sealing be placed on microlayer model generate chip on be installed in place, guarantee microlayer model generate chip on each hole
Position is all by sealed envelope；
S2, chip load；
S21, ready microlayer model chip is fixed on chip fixture；
S22, driving motor movement, drive chip fixture to move to required position on the slide rail；
The generation of S3, microlayer model:
S31, cylinder drive pressure control mould group mobile to chip fixture direction, up to pressing chip sealing cover；
S32, pressure control mould group start to start work, and the high-precision gas pressure that pressure control mould group generates to microlayer model generates
Chip sample hole and oilhole, aqueous sample and oily phase reagent enter the microlayer model that chip interior generates stable uniform by fluid channel
Emulsion；
S33, generation microlayer model emulsion pass through fluid channel flow into chip drop collect hole；
After the completion of S34, drop generate, air pressure module from service, microlayer model generation chip inlet gas pressure is unloaded to and atmospheric pressure
It is identical；
S4, microlayer model collect transfer:
After S41, microlayer model generate, cylinder driving pressure control mould group is moved upwards, and driving motor drives pallet to overhanging
Out, microlayer model chip and fixture are taken out, pallet is withdrawn and completes entire process by driving motor.
10. a kind of full-automatic microlayer model emulsion production method as claimed in claim 9, it is characterised in that: in the S34 when
When oily phase reagent specific gravity is higher than drop, by loading negative pressure in chip oil phase reagent entrance, drop is collected to the oil of hole bottom
Phase reagent suction part is to oily phase reagent entrance.
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| CN201811194968.7A CN109289949B (en) | 2018-10-15 | 2018-10-15 | Full-automatic micro-droplet emulsion generation device and generation method thereof |
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| CN201811194968.7A CN109289949B (en) | 2018-10-15 | 2018-10-15 | Full-automatic micro-droplet emulsion generation device and generation method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110205242A (en) * | 2019-06-18 | 2019-09-06 | 苏州锐讯生物科技有限公司 | It is a kind of to fast implement the micro-fluidic chip component and its application that digital pcr reacts |
| CN110339881A (en) * | 2019-07-30 | 2019-10-18 | 中国科学院深圳先进技术研究院 | A kind of miniflow drop formation device |
| CN110373310A (en) * | 2019-08-09 | 2019-10-25 | 上海烈冰生物医药科技有限公司 | A kind of micro-fluidic unicellular sorting unit based on Pneumatic Monitoring System |
| CN110523448A (en) * | 2019-09-03 | 2019-12-03 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Droplet preparation system and preparation method |
| CN110624613A (en) * | 2019-08-19 | 2019-12-31 | 昆山汇先医药技术有限公司 | Automatic microfluidic sample processing equipment and control method thereof |
| WO2020207295A1 (en) * | 2019-04-09 | 2020-10-15 | 京东方科技集团股份有限公司 | Reaction device and reaction system for detection chip |
| WO2021016870A1 (en) * | 2019-07-30 | 2021-02-04 | 中国科学院深圳先进技术研究院 | Micro-flow droplet generation device |
| CN114887674A (en) * | 2022-04-26 | 2022-08-12 | 北京百迈客生物科技有限公司 | Micro-droplet generating device |
| US11654435B2 (en) | 2019-03-29 | 2023-05-23 | Boe Technology Group Co., Ltd. | Detection chip, method for operating detection chip, and reaction system |
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