CN216614688U - Open nucleic acid under integration is carried test tube soon and is carried and detection device soon - Google Patents

Open nucleic acid under integration is carried test tube soon and is carried and detection device soon Download PDF

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Publication number
CN216614688U
CN216614688U CN202121181581.5U CN202121181581U CN216614688U CN 216614688 U CN216614688 U CN 216614688U CN 202121181581 U CN202121181581 U CN 202121181581U CN 216614688 U CN216614688 U CN 216614688U
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nucleic acid
magnetic
zone
elution
area
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王德明
周杰锋
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Ningbo Kangcheng Deno Biomedical Co ltd
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Ningbo Kangcheng Deno Biomedical Co ltd
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Abstract

The utility model relates to the field of nucleic acid extraction and detection, in particular to an integrated lower-opening nucleic acid quick-lifting test tube and a quick-lifting and detecting device. The test tube comprises a main tube; the inner cavity of the main pipe is sequentially provided with a cracking area, a washing area and an elution area from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking zone and the washing zone and between the washing zone and the elution zone for separation, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone and the elution zone; the inner cavity of the main pipe is also provided with a magnetic track extending from the cracking area to the elution area, the lower end of the magnetic track is integrally formed with the main pipe and is provided with an opening communicated with the outside for the magnetic rod to extend into, and the upper end of the magnetic track is closed and is positioned in the elution area; the top end of the main pipe is provided with a pipe cover, and the pipe cover comprises a cover body, a storage tank and a sealing cover; the test tube drives a large number of magnetic beads once, so that the nucleic acid extraction efficiency is improved; meanwhile, the magnetic beads are uniformly distributed on the outer wall of the magnetic track with larger area and are not stacked, so that the magnetic beads are more stable in movement.

Description

Open nucleic acid under integration is carried test tube soon and is carried and detection device soon
Technical Field
The utility model relates to the field of nucleic acid extraction, in particular to an integrated lower-opening nucleic acid rapid-extraction test tube.
Background
Along with the popularization of gene detection, personalized drug delivery, prenatal diagnosis and the like, the traditional DNA extraction method is more and more obviously limited today when high flux and automation are pursued in various fields of the biological industry. Because the magnetic bead method for extracting nucleic acid can realize automatic extraction and large-scale operation, and has simple operation and short time, the magnetic bead method for extracting nucleic acid is more and more emphasized.
The Chinese invention patent application (publication No. CN108796038B, published: 20191018) discloses a nucleic acid integrated detection method and a detection reagent tube, wherein a plurality of separating plugs which are arranged up and down are arranged in a main tube, and a liquid-phase or solid-phase hydrophobic layer is arranged on each separating plug, so that lysate, cleaning liquid and reaction liquid in the detection reagent tube are isolated; adding a sample into a lysis solution for uniform mixing and lysis, extracting nucleic acid in the sample by using nano magnetic beads, driving the nano magnetic beads to carry the nucleic acid to sequentially pass through each hydrophobic layer along magnetic bead channels on the inner wall of a detection reagent tube by using an external magnetic body to enter cleaning liquid and reaction solution, and realizing the cleaning and amplification of the nucleic acid, wherein a biological reagent required by the reaction solution is stored in a separation plug above the reaction solution, and finally, an external device realizes the detection of the sample nucleic acid through optical detection, thereby realizing multiple steps of nucleic acid extraction, cleaning and amplification reaction in the same detection reagent tube. The utility model has the characteristics of reducing detection errors and reducing operation difficulty.
The prior art has the following defects: the magnetic bead channel sets up and is being responsible for the inner wall and quantity less, and magnetic bead channel width is narrower, and the magnetic rod is less from being responsible for the magnetic bead quantity that the outer wall once drove to cause nucleic acid extraction efficiency lower. Meanwhile, the width of the magnetic bead channel is narrow, so that the magnetic beads can be easily stacked and cannot be uniformly distributed on the inner wall of the magnetic bead channel, and the magnetic beads are not stable in movement.
Disclosure of Invention
The purpose of the utility model is: aiming at the problems, the magnetic track is arranged in the main pipe, and the magnetic rod is inserted into the inner hole of the magnetic track to drive the magnetic bead to be attached to the outer wall of the magnetic track and move along with the magnetic rod; therefore, the magnetic beads can move downwards without being limited by the number of magnetic bead channels, a large number of magnetic beads are driven at one time, and the nucleic acid extraction efficiency is improved; simultaneously, the magnetic bead evenly distributed does not pile up each other on the great magnetic track outer wall of area for opening nucleic acid under the more steady integration when the magnetic bead moves carries the test tube soon.
In order to achieve the purpose, the utility model adopts the following technical scheme:
an integrated lower opening nucleic acid quick-extracting test tube, which comprises a main tube; the inner cavity of the main pipe is sequentially provided with a cracking area, a washing area and an elution area from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking zone and the washing zone and between the washing zone and the elution zone for separation, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone and the elution zone; the inner cavity of the main pipe is also provided with a magnetic track extending from the cracking zone to the elution zone, the lower end of the magnetic track is integrally formed with the main pipe and is provided with an opening communicated with the outside for the magnetic rod to extend into, and the upper end of the magnetic track is closed and is positioned in the elution zone; the top end of the main pipe is provided with a pipe cover, and the pipe cover comprises a cover body, a storage tank and a sealing cover; the sealing cover is positioned at the top end opening of the storage tank and seals the storage tank; the storing tank is provided with blanking hole in the bottom, and the storing tank is linked together through blanking hole and the schizolysis district of being responsible for to blanking downthehole being provided with the tinfoil and sealing it.
Preferably, the sealing cover is made of elastic material, and the sealing cover is in interference fit with the storage tank to close the top end opening of the storage tank. The sealing cover is connected with the cover body through a bendable material.
Preferably, the main pipe inner cavity is provided with a first separating plug and a second separating plug; annular gaps are formed among the outer wall of the magnetic track, the first separating plug and the second separating plug; the hydrophobic sealing layers are respectively positioned in the first separating plug and the second separating plug and fill and seal the inner cavity of the main pipe at the position.
Preferably, the hydrophobic sealing layer is a paraffin or a liquid-phase hydrophobic layer. The first and second plugs are both of an elastomeric material.
Preferably, a plurality of partition plates are arranged in the elution area, and the partition plates are respectively connected with the outer wall of the magnetic track and the inner wall of the main pipe cavity at the two radial ends so as to divide the elution area into a plurality of branch pipes.
Preferably, the bottom of the elution area is provided with a base, and a liquid outlet at the bottom of the elution area is an opening and is communicated with the outside; the base is provided with a collecting hole and a magnetic track connecting hole, the collecting hole is communicated with a liquid outlet at the bottom of the elution area, and the upper end of the collecting hole is embedded with the outer wall of the liquid outlet at the bottom of the elution area; the opening at the lower end of the magnetic track is communicated with the outside through a magnetic track connecting hole, and the magnetic track connecting hole is matched with the outer wall of the magnetic rod. A plurality of ribs are vertically and fixedly arranged on the inner wall of the cracking zone, and the ribs protrude out of the inner wall of the cracking zone; the outer wall of the base is provided with threads, and the threads on the outer wall of the base are screwed with the threaded cover.
In addition, the utility model also discloses an integrated lower opening nucleic acid rapid-extraction and detection device, which comprises the integrated nucleic acid rapid-extraction test tube and a magnetic rod, wherein the outer wall of the magnetic rod is matched with the inner hole of the magnetic track.
The integrated lower opening nucleic acid quick-extraction test tube adopting the technical scheme has the advantages that:
the hydrophobic sealing layer separates cell lysis solution in the lysis zone from magnetic bead washing solution in the washing zone and separates magnetic bead washing solution in the washing zone from nucleic acid eluent in the elution zone; after the magnetic rod is placed into the inner hole of the magnetic track from the opening at the bottom end of the magnetic track, the magnetic beads in the cracking area are sucked and attached to the outer wall of the magnetic track by using the magnetic force of the magnetic rod; the magnetic bar moves downwards to drive the magnetic beads to move downwards to penetrate through the hydrophobic sealing layer to enter a washing area and an elution area respectively for washing and elution so as to complete the extraction of nucleic acid; in the mode, the magnetic beads are attached to the outer wall of the magnetic track and move along with the magnetic rod, the outer wall of the whole magnetic track can be used for moving the magnetic beads without being limited by the number of magnetic bead channels, a large number of magnetic beads can be driven at one time, and the nucleic acid extraction efficiency is improved; meanwhile, the magnetic beads are uniformly distributed on the outer wall of the magnetic track with larger area and are not stacked, so that the magnetic beads are more stable in movement.
Drawings
FIG. 1 is a schematic structural diagram of the present invention.
Detailed Description
The following describes in detail embodiments of the present invention with reference to the drawings.
Example 1
An integrated lower opening nucleic acid quick-extraction test tube as shown in FIG. 1, which comprises a main tube 1; the inner cavity of the main pipe 1 is sequentially provided with a cracking area 2, a washing area 4 and an elution area 6 from top to bottom; hydrophobic sealing layers are respectively arranged between the cracking zone 2 and the washing zone 4 and between the washing zone 4 and the elution zone 6 for separation, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone 4 and the elution zone 6; the inner cavity of the main pipe 1 is also provided with a magnetic track 7 extending from the cracking zone 2 to the elution zone 6, the lower end of the magnetic track 7 is integrally formed with the main pipe 1 and is provided with an opening communicated with the outside for a magnetic rod to extend into, and the upper end of the magnetic track 7 is closed and is positioned in the elution zone 6; the top end of the main pipe 1 is provided with a pipe cover 180, and the pipe cover 180 comprises a cover body 182, a storage tank 183 and a sealing cover 184; the sealing cover 184 is positioned at the top end of the storage groove 183 and is opened and closed; the bottom of the storage tank 183 is provided with a blanking hole 185, the storage tank 183 is communicated with the cracking zone 2 of the main pipe 1 through the blanking hole 185, and the blanking hole 185 is internally provided with tinfoil to seal the storage tank. In this way, the hydrophobic sealing layer separates the cell lysis solution in the lysis zone 2 from the magnetic bead washing solution in the washing zone 4, and separates the magnetic bead washing solution in the washing zone 4 from the nucleic acid eluent in the elution zone 6; after the magnetic rod is placed into an inner hole of the magnetic track 7 from the opening at the bottom end of the magnetic track 7, the magnetic bead in the cracking area 2 is sucked and attached to the outer wall of the magnetic track 7 by using the magnetic force of the magnetic rod; the magnetic bar moves downwards to drive the magnetic beads to move downwards to pass through the hydrophobic sealing layer to enter the washing area 4 and the elution area 6 respectively for washing and elution so as to complete the extraction of nucleic acid; in this way, the magnetic beads are attached to the outer wall of the magnetic track 7 and move along with the magnetic rod, the outer wall of the whole magnetic track 7 can be used for moving the magnetic beads without being limited by the number of magnetic bead channels, a large number of magnetic beads can be driven at one time, and the nucleic acid extraction efficiency is improved; meanwhile, the magnetic beads are uniformly distributed on the outer wall of the magnetic track with larger area and are not stacked, so that the magnetic beads are more stable in movement. Furthermore, the magnetic beads or proteinase k can be preset in the storage tank 183 and can be added by directly poking the tinfoil, so that an operator does not need to suck and move the magnetic beads or proteinase k from other positions again, and the operation steps are reduced. Meanwhile, the magnetic beads or proteinase k preset in the storage tank 183 can be transported and stored simultaneously with the main pipe 1 without being transported and stored separately, so that the transportation and storage processes are simplified, and the integrated extraction of nucleic acid is facilitated.
The sealing cover 184 is made of elastic material, and the top end of the storage groove 183 is closed by the interference fit of the sealing cover 184 and the storage groove 183. The sealing cover 184 is connected with the cover 182 by a bendable material.
The inner cavity of the main pipe 1 is provided with a first separating plug and a second separating plug, so that magnetic beads can be attached to the outer wall of the magnetic track 7 and move downwards after passing through the annular gap; annular gaps are formed between the outer wall of the magnetic track 7 and the first separating plug and the second separating plug; the hydrophobic sealing layers are respectively positioned in the first separating plug and the second separating plug and fill and seal the cavity in the main pipe 1 at the position so as to be beneficial to positioning the solid hydrophobic sealing layers.
The hydrophobic sealing layer is a paraffin or liquid-phase hydrophobic layer. The first and second plugs are both of an elastomeric material. The first separating plug and the second separating plug can be clamped on the inner wall of the main pipe 1 by utilizing the elasticity of the first separating plug and the second separating plug and the limit of the inner wall of the main pipe 1.
A plurality of clapboards are arranged in the elution area 6, and the radial two ends of the clapboards are respectively connected with the outer wall of the magnetic track 7 and the inner wall of the cavity of the main pipe 1 so as to divide the elution area 6 into a plurality of branch pipes. The elution liquid is divided into a plurality of mutually independent parts by the branch pipes, so that the control analysis can be simultaneously carried out on the plurality of parts of elution liquid, the influence of accidental factors is eliminated, and the accuracy of nucleic acid detection is improved.
A base 60 is arranged at the bottom of the elution area 6, and a liquid outlet at the bottom of the elution area 6 is open and is communicated with the outside; the base 60 is provided with a collecting hole 64 and a magnetic track connecting hole 65, the collecting hole 64 is communicated with a liquid outlet at the bottom of the elution zone 6, and the upper end of the collecting hole is embedded with the outer wall of the liquid outlet at the bottom of the elution zone 6; the track lower end opening communicates with the outside through a track connection hole 65 and the track connection hole 65 is fitted to the outer wall of the magnetic rod. After the nucleic acid eluent in the elution area 6 contacts the washed magnetic beads to elute the nucleic acid combined on the magnetic beads to the nucleic acid eluent in the elution area 6, the external device detects the sample nucleic acid through optical detection or pulls the base 60 from the elution area 6 of the main pipe 1, collects the solution in the base 60 and takes the solution to other devices to perform nucleic acid detection, and the integrated process of quickly extracting and detecting the nucleic acid is completed. A plurality of ribs 21 are vertically and fixedly arranged on the inner wall of the cracking zone 2, and the ribs 21 protrude out of the inner wall of the cracking zone 2, so that the ribs 21 can help uniformly mix when the solution in the cracking zone 2 is vibrated; the outer wall of the base 60 is provided with threads, and the threads on the outer wall of the base 60 are screwed with the threaded cover so as to close the opening of the disassembled elution area 6.
In addition, the utility model also discloses an integrated nucleic acid quick-lifting test tube with a lower opening, which comprises the integrated nucleic acid quick-lifting test tube and a magnetic rod, wherein the outer wall of the magnetic rod is matched with the inner hole of the magnetic track 7.
When in use: 1) presetting lysis solution in the lysis zone 2, presetting magnetic beads or proteinase k or a mixture of the magnetic beads and the proteinase k into a storage tank 183, opening a sealing cover 184, breaking a tinfoil at the position of a blanking hole 185, and adding sample cells to be detected into the lysis zone 2; the magnetic beads or the proteinase k or the mixture of the magnetic beads and the proteinase k also fall into the lysis zone 2 along the blanking hole 185, and the sample cells are lysed by the lysis solution to release complete nucleic acids and are combined with the magnetic beads; or the tinfoil at the 185 position of the blanking hole is directly broken and the solution containing the sample cells and magnetic beads to be detected after the lysis is added into the lysis zone 2 for the lysis; 2) inserting a magnetic rod into the cracking zone 2 from the opening at the lower end of the magnetic track 7, moving the magnetic rod downwards to the washing zone 4, attracting magnetic beads to be attached to the outer wall of the magnetic track 7 and moving the magnetic rod downwards to the washing zone 4 after passing through a hydrophobic sealing layer; when the hydrophobic sealing layer is paraffin, the main pipe 1 is heated to melt the paraffin; 3) after the magnetic bead washing liquid removes some impurities such as protein and inorganic salt in the nucleic acid combined with the magnetic beads, the magnetic rod drives the magnetic beads to continuously move downwards to pass through the hydrophobic sealing layer in the second separating plug to reach the elution area 6; 4) contacting the nucleic acid eluent with the washed magnetic beads to elute the nucleic acids combined on the magnetic beads into the nucleic acid eluent in the elution area 6; 5) carrying out PCR or isothermal amplification reaction on nucleic acid in nucleic acid eluent, introducing a molecular beacon into the nucleic acid eluent, enabling external equipment to observe the PCR or isothermal amplification reaction in real time through fluorescent quantitative detection, and obtaining the quantity of sample nucleic acid by analyzing a fluorescence value; or after the base 60 is pulled out from the elution area 6 of the main pipe 1, the thread on the outer wall of the base 60 is screwed with the thread cover so as to close the opening of the elution area 6 and carry the nucleic acid to other equipment for nucleic acid detection, thereby completing the integrated process of quickly extracting and detecting the nucleic acid.

Claims (11)

1. An integrated lower opening nucleic acid quick-extracting test tube, which comprises a main tube (1); the inner cavity of the main pipe (1) is sequentially provided with a cracking area (2), a washing area (4) and an elution area (6) from top to bottom; hydrophobic sealing layers are respectively arranged between the lysis zone (2) and the washing zone (4) and between the washing zone (4) and the elution zone (6) for separating, and magnetic bead washing liquid and nucleic acid eluent are respectively arranged in the washing zone (4) and the elution zone (6); the device is characterized in that the inner cavity of the main pipe (1) is also provided with a magnetic track (7) extending from the cracking zone (2) to the elution zone (6), the lower end of the magnetic track (7) is integrally formed with the main pipe (1) and is provided with an opening communicated with the outside for the magnetic rod to extend into, and the upper end of the magnetic track (7) is closed and is positioned in the elution zone (6); the top end of the main pipe (1) is provided with a pipe cover (180), and the pipe cover (180) comprises a cover body (182), a storage tank (183) and a sealing cover (184); the sealing cover (184) is positioned at the top end of the storage groove (183) and is closed; the bottom of the storage tank (183) is provided with a blanking hole (185), the storage tank (183) is communicated with the cracking area (2) of the main pipe (1) through the blanking hole (185), and the blanking hole (185) is internally provided with tinfoil to seal the blanking hole.
2. The integrated bottom-opening nucleic acid rapid-extraction test tube according to claim 1, wherein the sealing cover (184) is made of elastic material, and the sealing cover (184) is in interference fit with the storage groove (183) to close the top end opening of the storage groove (183).
3. The integrated lower opening nucleic acid rapid-extraction tube of claim 1, wherein the sealing cover (184) is connected with the cover body (182) by a bendable material.
4. The integrated lower opening nucleic acid rapid-extraction test tube according to claim 1, wherein the inner cavity of the main tube (1) is provided with a first separating plug and a second separating plug; annular gaps are formed between the outer wall of the magnetic track (7) and the first separating plug and the second separating plug; the hydrophobic sealing layer is respectively positioned in the first separating plug and the second separating plug and fills and seals the inner cavity of the main pipe (1) at the position.
5. The integrated bottom-opening nucleic acid rapid-lift tube according to claim 1, wherein the hydrophobic sealing layer is paraffin or a liquid-phase hydrophobic layer.
6. The integrated bottom-opening nucleic acid rapid-extraction tube of claim 3, wherein the first and second separating plugs are made of elastic material.
7. The integrated lower opening nucleic acid rapid-extraction test tube according to claim 1, wherein a plurality of partition plates are arranged in the elution area (6), and the plurality of partition plates are respectively connected with the outer wall of the magnetic track (7) and the inner wall of the cavity of the main tube (1) at two radial ends so as to divide the elution area (6) into a plurality of branch tubes.
8. The integrated lower opening nucleic acid rapid-extraction test tube according to claim 1, wherein a base (60) is arranged at the bottom of the elution area (6), and a liquid outlet at the bottom of the elution area (6) is open and is communicated with the outside; the base (60) is provided with a collecting hole (64) and a magnetic track connecting hole (65), the collecting hole (64) is communicated with a liquid outlet at the bottom of the elution area (6), and the upper end of the collecting hole is embedded with the outer wall of the liquid outlet at the bottom of the elution area (6); the lower end opening of the magnetic track is communicated with the outside through a magnetic track connecting hole (65), and the magnetic track connecting hole (65) is matched with the outer wall of the magnetic rod.
9. The integrated bottom-opening nucleic acid rapid-extraction test tube according to claim 1, wherein a plurality of ribs (21) are vertically and fixedly arranged on the inner wall of the lysis zone (2), and the plurality of ribs (21) protrude out of the inner wall of the lysis zone (2).
10. The integrated bottom-opening nucleic acid rapid-extraction test tube according to claim 1, wherein the outer wall of the base (60) is provided with threads, and the threads on the outer wall of the base (60) are screwed with the threaded cap.
11. An integrated lower opening nucleic acid rapid-extraction and detection device, which is characterized by comprising an integrated nucleic acid rapid-extraction test tube and a magnetic rod according to any one of claims 1 to 10, wherein the outer wall of the magnetic rod is matched with an inner hole of a magnetic track (7).
CN202121181581.5U 2021-05-28 2021-05-28 Open nucleic acid under integration is carried test tube soon and is carried and detection device soon Active CN216614688U (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113174317A (en) * 2021-05-28 2021-07-27 宁波康程德诺生物医药有限公司 Integrated upper-opening nucleic acid quick-extraction test tube, quick-extraction detection device and method
CN113186098A (en) * 2021-05-28 2021-07-30 宁波康程德诺生物医药有限公司 Integrated lower opening nucleic acid quick-extraction test tube, quick-extraction detection device and method
CN113174317B (en) * 2021-05-28 2024-03-22 宁波康程德诺生物医药有限公司 Integrated upper opening nucleic acid quick-lifting test tube, quick-lifting detection device and method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113174317A (en) * 2021-05-28 2021-07-27 宁波康程德诺生物医药有限公司 Integrated upper-opening nucleic acid quick-extraction test tube, quick-extraction detection device and method
CN113186098A (en) * 2021-05-28 2021-07-30 宁波康程德诺生物医药有限公司 Integrated lower opening nucleic acid quick-extraction test tube, quick-extraction detection device and method
CN113174317B (en) * 2021-05-28 2024-03-22 宁波康程德诺生物医药有限公司 Integrated upper opening nucleic acid quick-lifting test tube, quick-lifting detection device and method

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