CN104147309A - Dunaliella salina nutrition composition and preparation method thereof - Google Patents
Dunaliella salina nutrition composition and preparation method thereof Download PDFInfo
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Abstract
The invention provides a Dunaliella salina nutrition composition and a preparation method thereof. The composition consists of, by weight, 50-70 parts of natural carotene, 7-15 parts of cod-liver oil, 5-10 parts of ginseng saponin extract, 4-6 parts of yam extract, 3-5 parts of oyster extract and 1-2 parts of natural indigo extract. The ginseng saponin extract is a methanol extract; the yam extract is an ethanol extract; the oyster extract is an enzymatic extract; the natural indigo extract is an indigo lipophilic extract. The composition is prepared by mixing the extracts; the composition has balanced nutrition, can significantly enhance the body's immune function, delay aging, and obviously improve clinical symptoms of patients with hypertension and diabetes, and is especially suitable for the elderly.
Description
Technical Field
The invention relates to a nutrient solution and a preparation method thereof, in particular to a dunaliella salina nutrient composition and a preparation method thereof.
Background
Dunaliella salina (Dunaliella salina) is mainly distributed in the coast of the Mediterranean sea, and the high-salt water in the inland of China also produces Dunaliella salina, and in addition, the middle and western plateau desert also has large and small salt lakes. The Dunaliella salina is a unicellular organism, and has a cell nucleus, two equally long terminal flagella, a chromoplast cup, a larger protein nucleus at the proximal part, and a large eyespot at the front end of the cell. Because it has no cellulose cell wall, the shape of the sports bag is different, such as pear shape, ellipse shape, long neck shape, etc. The Dunaliella salina performs asexual reproduction, and a swimming cell is longitudinally split into two daughter cells. The invention patent ZL 95115709.4 discloses that Gobi Dunaliella salina has rich nutritive value, and can provide multiple carotenes, vitamins and minerals for human beings after being obtained by a specific process. However, the product obtained by the method is mainly natural carotene, the demand of substances such as linolenic acid, lecithin and the like is still to be improved, and the preparation process has the technical problems of low extraction rate and low reactant utilization rate. In view of this, the search for a nutrient composition of dunaliella salina with more balanced nutrition and a preparation process thereof has important practical significance.
Disclosure of Invention
The invention aims to provide a preparation method of a dunaliella salina nutrient composition.
In order to solve the technical problems, the technical scheme of the invention is as follows:
a dunaliella salina nutrient composition comprises, by weight, 50-70 parts of natural carotene, 7-15 parts of cod liver oil, 5-10 parts of ginsenoside extract, 4-6 parts of Chinese yam extract, 3-5 parts of oyster extract and 1-2 parts of indigo naturalis extract, wherein the ginsenoside extract is a methanol extract, the Chinese yam extract is an ethanol extract, the oyster extract is an enzymolysis extract and the indigo naturalis extract is a fat-soluble extract.
Preferably, the natural carotene of the nutrient composition of dunaliella salina is prepared by the following method:
(1) fully mixing a saline algae solution and a ferric chloride solution with the concentration of 5% in a mixing procedure according to the volume ratio of 1:0.006 until algae iron flocs stably float upwards to obtain a mixed solution;
(2) introducing gas-dissolving water (namely water dissolved with oxygen under pressure) into the mixed solution, wherein the volume ratio of the mixed solution to the gas-dissolving water is 70:30, the algae iron flocs in the mixed solution are supported and floated by water vapor beads released by the gas-dissolving water after air flotation separation, static separation of the mixed solution is realized, algae paste is obtained, and the separated water returns to the culture pond to continuously culture dunaliella salina;
(3) the algae paste enters an extraction process, ethyl acetate is used as an extracting agent, the volume ratio of the algae paste to the ethyl acetate is 1:0.5-1, the extraction temperature is 40-50 ℃, the extraction time is 30-50 minutes/time, clear liquid (the extract is refined salt algae liquid) and slag pulp are separated from the extract through a disc type separator, the slag pulp enters a second extraction and separation process, the extraction conditions are the same as the first extraction, and the clear liquid and the slag pulp obtained by the two extraction and separation processes are respectively combined;
(4) filtering the clear liquid by a 100-200 mesh titanium tube filter to remove floating substances, and then feeding the clear liquid into an evaporation kettle to evaporate the clear liquid in an electric heating automatic closed circulation mode to obtain a concentrated solution (namely the target product natural carotene).
Preferably, in the above nutrient composition of dunaliella salina, the content of the dunaliella salina in the dunaliella salina solution in the step (1) is 10-50 ten thousand cell lines/ml.
Preferably, the dunaliella salina nutritional composition, the residue slurry obtained in the step (3) can be used as fish and shrimp feed.
Preferably, in the above-mentioned nutrient composition of dunaliella salina, the concentration of carotene in the concentrated solution in step (4) is 25% -35%, the linolenic acid content is 6330mg/100g to 6850mg/100g of concentrated solution, and the lecithin content is 2770mg/100g to 3160mg/100g of concentrated solution.
Preferably, the above dunaliella salina nutrient composition, the ginsenoside extract is prepared by the following method:
(1) after the ginseng root coarse powder is sieved by a sieve of 60-100 meshes, heating and refluxing the ginseng root coarse powder by methanol until the reaction of antimony trichloride chloroform saturated solution is negative;
(2) recovering methanol from the extractive solution under reduced pressure, and dissolving the obtained extract with 10 times of distilled water;
(3) defatting the water solution with diethyl ether in separating funnel for 4 times, extracting the water layer with water-saturated n-butanol for 6 times, and mixing n-butanol solutions;
(4) recovering n-butanol under reduced pressure, and evaporating to obtain ginsenoside extract.
Preferably, the above nutrient composition of dunaliella salina extract is prepared by the following steps:
(1) adding 95% ethanol into the yam decoction pieces, performing reflux extraction for 2 times at 100 ℃, wherein each time lasts for 1 hour, and the mass volume ratio of the yam decoction pieces to the 95% ethanol is 1: 10, filtering, repeatedly extracting filter residue for 3 times by using 95% ethanol, wherein each time is 4 hours, and the mass volume ratio of the filter residue to the 95% ethanol is 1: 15, filtering;
(2) mixing all the filtrates obtained in the step (1), placing the filtrates at room temperature for centrifugation, and removing starch precipitate;
(3) concentrating the centrifugate to 1/3, adding anhydrous ethanol until the ethanol concentration is 80%, standing overnight, and centrifuging; and (5) drying the precipitate to obtain the yam extract.
Preferably, in the above nutrient composition of dunaliella salina, the oyster extract is prepared by the following steps:
(1) adding fresh oyster meat into precooled double distilled water, wherein the mass ratio of the oyster meat to the double distilled water is 1:3, and homogenizing at high speed to obtain homogenate;
(2) adding trypsin into the homogenate, wherein the adding amount of the trypsin is 2% of the weight of the homogenate, adjusting the pH to 8.0 by using 0.2mol/L sodium hydroxide, carrying out enzymolysis in a water bath at 45 ℃ for 6h, inactivating in a boiling water bath for 10min, cooling, centrifuging at a low temperature of 7000r/min for 30min, and taking a supernatant;
(3) freeze drying the supernatant to obtain Concha Ostreae extract.
Preferably, the natural indigo extract of the saline algae nutrient composition is prepared by the following method:
(1) extracting indigo naturalis with water by percolation method to obtain water soluble components, drying the residue, placing into round bottom flask, reflux extracting with 10 times of 95% ethanol for 3 times and 4 hr/time, and mixing extractive solutions;
(2) concentrating the extractive solution under reduced pressure to obtain viscous paste, volatilizing ethanol in 70 deg.C water bath, and vacuum drying at 40 deg.C for 24 hr to obtain indigo naturalis extract.
Preferably, the dunaliella salina nutrient composition comprises 65 parts by weight of natural carotene, 12 parts by weight of cod liver oil, 7 parts by weight of ginsenoside extract, 6 parts by weight of Chinese yam extract, 4 parts by weight of oyster extract and 1.5 parts by weight of natural indigo extract.
The preparation method of the dunaliella salina nutrient composition comprises the following specific steps:
(1) weighing natural carotene, cod liver oil, ginsenoside extract, Chinese yam extract, oyster extract and natural indigo extract in formula ratio as raw materials;
(2) fully mixing natural carotene, cod liver oil and the indigo naturalis extract at 50-70 ℃;
(3) cooling to 30-40 ℃, and continuously adding the ginsenoside extract and the yam extract for fully mixing;
(4) cooling to 10-20 ℃, continuously adding the oyster extract, and uniformly mixing to obtain the oyster extract.
The invention has the beneficial effects that:
the dunaliella salina nutrient composition has balanced nutrition, can obviously enhance the immunologic function of a human body and delay senility, has obvious improvement effect on the clinical symptoms of patients with hypertension and diabetes, and is particularly suitable for middle-aged and elderly people to take; the main component natural carotene is prepared by a specific method, has higher carotene, linolenic acid and lecithin content, and effectively improves the health-care effect of the natural carotene by the synergistic effect of other components; the preparation method of the composition is simple and is suitable for the requirement of large-scale industrial production.
Detailed Description
The technical solution of the present invention is further described with reference to the following specific examples.
Example 1
A Dunaliella salina nutritional composition comprises natural carotene, cod liver oil, ginsenoside extract, rhizoma Dioscoreae extract, Concha Ostreae extract and indigo naturalis extract, wherein the natural carotene 65kg, cod liver oil 12kg, ginsenoside extract 7kg, rhizoma Dioscoreae extract 6kg, Concha Ostreae extract 4kg, and indigo naturalis extract 1.5 kg.
The preparation method of the dunaliella salina nutrient composition comprises the following specific steps:
(1) weighing natural carotene, cod liver oil, ginsenoside extract, Chinese yam extract, oyster extract and natural indigo extract in formula ratio as raw materials;
(2) mixing natural carotene, cod liver oil and indigo naturalis extract at 60 deg.C;
(3) cooling to 35 deg.C, adding ginsenoside extract and rhizoma Dioscoreae extract, and mixing;
(4) cooling to 12 deg.C, adding Concha Ostreae extract, and mixing.
Example 2
A Dunaliella salina nutritional composition comprises natural carotene 70kg, cod liver oil 7kg, ginsenoside 10kg, rhizoma Dioscoreae extract 4kg, Concha Ostreae extract 5kg, and indigo naturalis extract 2 kg.
The preparation method of the dunaliella salina nutrient composition comprises the following specific steps:
(1) weighing natural carotene, cod liver oil, ginsenoside extract, Chinese yam extract, oyster extract and natural indigo extract in formula ratio as raw materials;
(2) mixing natural carotene, cod liver oil and indigo naturalis extract at 70 deg.C;
(3) cooling to 30 deg.C, adding ginsenoside extract and rhizoma Dioscoreae extract, and mixing;
(4) cooling to 10 deg.C, adding Concha Ostreae extract, and mixing.
Example 3
A Dunaliella salina nutritional composition comprises natural carotene, cod liver oil, ginsenoside extract, rhizoma Dioscoreae extract, Concha Ostreae extract and indigo naturalis extract, wherein the natural carotene 50kg, cod liver oil 15kg, ginsenoside extract 5kg, rhizoma Dioscoreae extract 6kg, Concha Ostreae extract 3kg, and indigo naturalis extract 1 kg.
The preparation method of the dunaliella salina nutrient composition comprises the following specific steps:
(1) weighing natural carotene, cod liver oil, ginsenoside extract, Chinese yam extract, oyster extract and natural indigo extract in formula ratio as raw materials;
(2) mixing natural carotene, cod liver oil and indigo naturalis extract at 50 deg.C;
(3) cooling to 40 deg.C, adding ginsenoside extract and rhizoma Dioscoreae extract, and mixing;
(4) cooling to 20 deg.C, adding Concha Ostreae extract, and mixing.
Example 4
A Dunaliella salina nutritional composition comprises natural carotene, cod liver oil, ginsenoside extract, rhizoma Dioscoreae extract, Concha Ostreae extract and indigo naturalis extract, wherein natural carotene 60kg, cod liver oil 9kg, ginsenoside extract 13kg, rhizoma Dioscoreae extract 5.5kg, Concha Ostreae extract 3.5kg, and indigo naturalis extract 2 kg.
The preparation method of the dunaliella salina nutrient composition comprises the following specific steps:
(1) weighing natural carotene, cod liver oil, ginsenoside extract, Chinese yam extract, oyster extract and natural indigo extract in formula ratio as raw materials;
(2) mixing natural carotene, cod liver oil and indigo naturalis extract at 70 deg.C;
(3) cooling to 40 deg.C, adding ginsenoside extract and rhizoma Dioscoreae extract, and mixing;
(4) cooling to 15 deg.C, adding Concha Ostreae extract, and mixing.
The amounts of the components described in examples 1-4 are increased or decreased according to the same proportion, and the obtained weight part relationship of the components all belongs to the protection scope of the invention. Wherein,
the natural carotene is prepared by the following method:
(1) taking a dunaliella salina solution (the content of the dunaliella salina is 10-50 ten thousand cell lines per milliliter and is selected from underground salt lake in Gansu region) in a culture pond and a ferric trichloride solution with the concentration of 5 percent according to the volume ratio of 1:0.006, and fully mixing the dunaliella salina solution and the ferric trichloride solution in the mixing process until the floccule of the dunaliella salina stably floats upwards to obtain a mixed solution;
(2) introducing gas-dissolving water (namely water dissolved with oxygen under pressure) into the mixed solution, wherein the volume ratio of the mixed solution to the gas-dissolving water is 70:30, the algae iron flocs in the mixed solution are supported and floated by water vapor beads released by the gas-dissolving water after air flotation separation, static separation of the mixed solution is realized, algae paste is obtained, and the separated water returns to the culture pond to continuously culture dunaliella salina;
(3) the algae paste enters an extraction process, ethyl acetate is used as an extracting agent, the volume ratio of the algae paste to the ethyl acetate is 1:0.7, the extraction temperature is 45 ℃, the extraction time is 40 minutes/time, clear liquid (the extract is refined salt algae liquid) and residue pulp are separated from the extract by a disc type separator, the residue pulp enters a second extraction and separation process, the extraction conditions are the same as the first time, the clear liquid and the residue pulp obtained by the two extraction and separation processes are respectively combined, and the obtained residue pulp can be used as fish and shrimp feed;
(4) filtering the clear liquid by a titanium tube filter of 160 meshes to remove floating substances, then feeding the clear liquid into an evaporation kettle, and evaporating the clear liquid in an electric heating automatic closed circulation mode to obtain a concentrated solution (namely the target product natural carotene), wherein the concentration of the carotene in the concentrated solution is 33.1 percent, the linolenic acid content of the concentrated solution is 6827 mg/100g, and the lecithin content of the concentrated solution is 3002mg/100 g.
The ginsenoside extract is prepared by the following method:
(1) after sieving the ginseng root coarse powder with a sieve of 80 meshes, heating and refluxing the ginseng root coarse powder by using methanol until the reaction of an antimony trichloride chloroform saturated solution is negative;
(2) recovering methanol from the extractive solution under reduced pressure, and dissolving the obtained extract with 10 times of distilled water;
(3) defatting the water solution with diethyl ether in separating funnel for 4 times, extracting the water layer with water-saturated n-butanol for 6 times, and mixing n-butanol solutions;
(4) recovering n-butanol under reduced pressure, and evaporating to obtain ginsenoside extract.
The Chinese yam extract is prepared by the following method:
(1) adding 95% ethanol into the yam decoction pieces, performing reflux extraction for 2 times at 100 ℃, wherein each time lasts for 1 hour, and the mass volume ratio of the yam decoction pieces to the 95% ethanol is 1: 10, filtering, repeatedly extracting filter residue for 3 times by using 95% ethanol, wherein each time is 4 hours, and the mass volume ratio of the filter residue to the 95% ethanol is 1: 15, filtering;
(2) mixing all the filtrates obtained in the step (1), placing the filtrates at room temperature for centrifugation, and removing starch precipitate;
(3) concentrating the centrifugate to 1/3, adding anhydrous ethanol until the ethanol concentration is 80%, standing overnight, and centrifuging; and (5) drying the precipitate to obtain the yam extract.
The oyster extract is prepared by the following method:
(1) adding fresh oyster meat into precooled double distilled water, wherein the mass ratio of the oyster meat to the double distilled water is 1:3, and homogenizing at high speed to obtain homogenate;
(2) adding trypsin into the homogenate, wherein the adding amount of the trypsin is 2% of the weight of the homogenate, adjusting the pH to 8.0 by using 0.2mol/L sodium hydroxide, carrying out enzymolysis in a water bath at 45 ℃ for 6h, inactivating in a boiling water bath for 10min, cooling, centrifuging at a low temperature of 7000r/min for 30min, and taking a supernatant;
(3) freeze drying the supernatant to obtain Concha Ostreae extract.
The indigo naturalis extract is prepared by the following method:
(1) extracting indigo naturalis with water by percolation method to obtain water soluble components, drying the residue, placing into round bottom flask, reflux extracting with 10 times of 95% ethanol for 3 times and 4 hr/time, and mixing extractive solutions;
(2) concentrating the extractive solution under reduced pressure to obtain viscous paste, volatilizing ethanol in 70 deg.C water bath, and vacuum drying at 40 deg.C for 24 hr to obtain indigo naturalis extract.
In the dunaliella salina nutrient composition, the natural carotene has the effects of maintaining the integrity of skin mucous membrane, protecting vision, resisting canceration, regulating immunity, delaying senility and the like; the cod liver oil mainly contains fat-soluble vitamin A, D, wherein vitamin A can promote formation of photochromic pigment in visual cells, and vitamin D can promote absorption of calcium by human body; the ginsenoside extract has the effects of enhancing immunity, resisting fatigue, improving memory and learning ability, promoting DNA and RNA synthesis, delaying aging, and improving myocardial ischemia and anoxia; the rhizoma Dioscoreae extract has effects of invigorating spleen, tonifying lung, invigorating kidney, replenishing vital essence, etc.; the Concha Ostreae extract has effects of suppressing hyperactive liver, subsiding yang, softening hard mass, resolving hard mass, astringing, and inducing astringency; the indigo naturalis extract has effects of clearing heat and detoxicating, cooling blood and arresting convulsion. The components have synergistic effect, effectively promote the in vivo absorption and utilization of natural carotene, and simultaneously have obvious improvement effect on the clinical symptoms of patients with hypertension and diabetes.
Application Experimental example 1
18 women aged 40-50 years old have basically normal physical examination indexes and no diabetes history, and non-tumor patients are randomly divided into two groups.
The first group took the dunaliella salina nutritional composition of the present invention, described in example 1, 500 mg/time, once a day;
the second group took 350 mg/time of the single ingredient natural carotene in the nutrient composition of dunaliella salina described in example 1 once a day.
The administration time is 3 months, then blood of the subject is collected, and the content of beta-carotene in the blood serum is determined by high performance liquid chromatography (method refers to determination of B-carotene in the blood serum by HPLC and relative bioavailability research thereof, and determination results are shown in Table 1.
TABLE 1
| Test group | First group | Second group |
| Average content | 315.6μg/L | 273.9μg/L |
Therefore, the dunaliella salina nutrient composition effectively promotes the absorption and utilization of natural carotene in vivo through the synergistic effect of other components.
Application Experimental example 2
45-55 years old, 30 type II diabetics were randomized into three groups.
The first group took the dunaliella salina nutritional composition of the present invention, described in example 1, 500 mg/time, once a day;
the second group took 350 mg/time of natural carotene as a single ingredient in the nutrient composition of dunaliella salina described in example 1 once a day;
the third group took glipizide tablets (Guangdong Pedi pharmaceutical Co., Ltd.) as hypoglycemic agent, 5 mg/time, three times a day. .
The administration time was 2 months, and the fasting blood glucose and postprandial blood glucose before and after administration were measured to show significant efficiency, and the results are shown in Table 2.
TABLE 2
| Test group | First group | Second group | Third group |
| Mean fasting blood glucose significant efficiency | 23％ | 19％ | 27％ |
| Mean postprandial blood glucose response | 24％ | 19％ | 30％ |
Application Experimental example 3
40 patients with hypertension of 60-70 years old are randomly divided into two groups, and are continuously taken with hypotensor according to normal daily dosage,
the first group took the dunaliella salina nutritional composition of the present invention, described in example 1, 500 mg/time, once a day;
the second group took 500 mg/time of the single ingredient natural carotene in the nutrient composition of dunaliella salina described in example 1 once a day.
The administration time was 1 month, and the average blood pressure decrease of the patients before and after the administration of the nutritional composition containing dunaliella salina and natural carotene was observed, and the results are shown in Table 3.
TABLE 3
| Test group | First group | Second group |
| Low pressure drop value | 21mmhg | 17mmhg |
| High pressure drop value | 25mmhg | 19mmhg |
The above detailed description of the dunaliella salina nutritional composition and the preparation method thereof with reference to the examples is illustrative and not restrictive, and several examples may be cited within the scope of the present invention, so that variations and modifications thereof without departing from the general concept of the present invention are within the scope of the present invention.
Claims (10)
1. A nutrient composition of a dunaliella salina, comprising: the composition comprises, by weight, 50-70 parts of natural carotene, 7-15 parts of cod liver oil, 5-10 parts of ginsenoside extract, 4-6 parts of yam extract, 3-5 parts of oyster extract and 1-2 parts of indigo naturalis extract, wherein the ginsenoside extract is a methanol extract, the yam extract is an ethanol extract, the oyster extract is an enzymolysis extract and the indigo naturalis extract is an indigo naturalis fat-soluble extract.
2. The salinomycin nutritional composition of claim 1, wherein: the natural carotene is prepared by the following method:
(1) fully mixing a saline algae solution and a ferric chloride solution with the concentration of 5% in a mixing procedure according to the volume ratio of 1:0.006 until algae iron flocs stably float upwards to obtain a mixed solution;
(2) introducing gas-dissolving water into the mixed solution, wherein the volume ratio of the mixed solution to the gas-dissolving water is 70:30, the algae iron flocs in the mixed solution are supported and floated by water vapor beads released by the gas-dissolving water after air flotation separation, static separation of the mixed solution is realized, algae paste is obtained, and the separated water returns to the culture pond to continuously culture the dunaliella salina;
(3) the algae paste enters an extraction process, ethyl acetate is used as an extracting agent, the volume ratio of the algae paste to the ethyl acetate is 1:0.5-1, the extraction temperature is 40-50 ℃, the extraction time is 30-50 minutes/time, clear liquid and residue pulp are separated from the extract liquid through a disc type separator, the residue pulp enters a second extraction and separation process, the extraction conditions are the same as the first extraction, and the clear liquid and the residue pulp obtained by the two extraction and separation processes are respectively combined;
(4) and filtering the clear liquid by using a 100-200-mesh titanium tube filter to remove floating substances, and then, evaporating the clear liquid in an evaporation kettle in an electric heating automatic closed circulation mode to obtain a concentrated solution, namely the target product natural carotene.
3. The salinomycin nutritional composition of claim 2, wherein: the content of the dunaliella salina in the dunaliella salina liquid in the step (1) is 10-50 ten thousand cell strains per milliliter.
4. The salinomycin nutritional composition of claim 2, wherein: the concentration of the carotene in the concentrated solution in the step (4) is 25-35%, the linolenic acid content of the concentrated solution is 6330mg/100g to 6850mg/100g of the concentrated solution, and the lecithin content of the concentrated solution is 2770mg/100g to 3160mg/100g of the concentrated solution.
5. The salinomycin nutritional composition of claim 1, wherein: the ginsenoside extract is prepared by the following method:
(1) after the ginseng root coarse powder is sieved by a sieve of 60-100 meshes, heating and refluxing the ginseng root coarse powder by methanol until the reaction of antimony trichloride chloroform saturated solution is negative;
(2) recovering methanol from the extractive solution under reduced pressure, and dissolving the obtained extract with 10 times of distilled water;
(3) defatting the water solution with diethyl ether in separating funnel for 4 times, extracting the water layer with water-saturated n-butanol for 6 times, and mixing n-butanol solutions;
(4) recovering n-butanol under reduced pressure, and evaporating to obtain ginsenoside extract.
6. The salinomycin nutritional composition of claim 1, wherein: the Chinese yam extract is prepared by the following method:
(1) adding 95% ethanol into the yam decoction pieces, performing reflux extraction for 2 times at 100 ℃, wherein each time lasts for 1 hour, and the mass volume ratio of the yam decoction pieces to the 95% ethanol is 1: 10, filtering, repeatedly extracting filter residue for 3 times by using 95% ethanol, wherein each time is 4 hours, and the mass volume ratio of the filter residue to the 95% ethanol is 1: 15, filtering;
(2) mixing all the filtrates obtained in the step (1), placing the filtrates at room temperature for centrifugation, and removing starch precipitate;
(3) concentrating the centrifugate to 1/3, adding anhydrous ethanol until the ethanol concentration is 80%, standing overnight, and centrifuging; and (5) drying the precipitate to obtain the yam extract.
7. The salinomycin nutritional composition of claim 1, wherein: the oyster extract is prepared by the following method:
(1) adding fresh oyster meat into precooled double distilled water, wherein the mass ratio of the oyster meat to the double distilled water is 1:3, and homogenizing at high speed to obtain homogenate;
(2) adding trypsin into the homogenate, wherein the adding amount of the trypsin is 2% of the weight of the homogenate, adjusting the pH to 8.0 by using 0.2mol/L sodium hydroxide, carrying out enzymolysis in a water bath at 45 ℃ for 6h, inactivating in a boiling water bath for 10min, cooling, centrifuging at a low temperature of 7000r/min for 30min, and taking a supernatant;
(3) freeze drying the supernatant to obtain Concha Ostreae extract.
8. The salinomycin nutritional composition of claim 1, wherein: the indigo naturalis extract is prepared by the following method:
(1) extracting indigo naturalis with water by percolation method to obtain water soluble components, drying the residue, placing into round bottom flask, reflux extracting with 10 times of 95% ethanol for 3 times and 4 hr/time, and mixing extractive solutions;
(2) concentrating the extractive solution under reduced pressure to obtain viscous paste, volatilizing ethanol in 70 deg.C water bath, and vacuum drying at 40 deg.C for 24 hr to obtain indigo naturalis extract.
9. The salinomycin nutritional composition of claim 1, wherein: the composition comprises, by weight, 65 parts of natural carotene, 12 parts of cod liver oil, 7 parts of ginsenoside extract, 6 parts of Chinese yam extract, 4 parts of oyster extract and 1.5 parts of natural indigo extract.
10. A process for preparing a nutrient composition of dunaliella salina according to claim 1, wherein: the preparation method comprises the following specific steps:
(1) weighing natural carotene, cod liver oil, ginsenoside extract, Chinese yam extract, oyster extract and natural indigo extract in formula ratio as raw materials;
(2) fully mixing natural carotene, cod liver oil and the indigo naturalis extract at 50-70 ℃;
(3) cooling to 30-40 ℃, and continuously adding the ginsenoside extract and the yam extract for fully mixing;
(4) cooling to 10-20 ℃, continuously adding the oyster extract, and uniformly mixing to obtain the oyster extract.
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| CN107427545A (en) * | 2015-04-09 | 2017-12-01 | 盖尔德玛公司 | Indigo naturalis extract and preparation method thereof |
| US20190160128A1 (en) | 2015-04-09 | 2019-05-30 | Galderma S.A. | Pharmaceutical composition comprising refined indigo naturalis extracts and the use thereof |
| US10668120B2 (en) | 2015-04-09 | 2020-06-02 | Galderma Sa | Antibacterial indigo naturalis or indigo-producing plant extract and use thereof |
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| CN1823849A (en) * | 2005-12-30 | 2006-08-30 | 徐贵义 | Extract of ferric trichloride and algae |
| CN103404757A (en) * | 2013-05-27 | 2013-11-27 | 胡安然 | Special diet for hyperglycemia patients |
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| CN1823849A (en) * | 2005-12-30 | 2006-08-30 | 徐贵义 | Extract of ferric trichloride and algae |
| CN103404757A (en) * | 2013-05-27 | 2013-11-27 | 胡安然 | Special diet for hyperglycemia patients |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107427545A (en) * | 2015-04-09 | 2017-12-01 | 盖尔德玛公司 | Indigo naturalis extract and preparation method thereof |
| EP3209313B1 (en) * | 2015-04-09 | 2018-12-19 | Galderma SA | An extract from indigo naturalis and a process for preparing the same |
| EP3482763A1 (en) * | 2015-04-09 | 2019-05-15 | Galderma SA | An extract from indigo naturalis and a process for preparing the same |
| US20190160128A1 (en) | 2015-04-09 | 2019-05-30 | Galderma S.A. | Pharmaceutical composition comprising refined indigo naturalis extracts and the use thereof |
| US10555985B2 (en) | 2015-04-09 | 2020-02-11 | Galderma S.A. | Pharmaceutical composition comprising refined Indigo naturalis extracts and the use thereof |
| US10668120B2 (en) | 2015-04-09 | 2020-06-02 | Galderma Sa | Antibacterial indigo naturalis or indigo-producing plant extract and use thereof |
| US10695391B2 (en) | 2015-04-09 | 2020-06-30 | Galderma S.A. | Extract from Indigo Naturalis and a process for preparing the same |
| US11116811B2 (en) | 2015-04-09 | 2021-09-14 | Galderma S.A. | Pharmaceutical composition comprising refined indigo naturalis extracts and the use thereof |
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