CN102688255A - Application of polysaccharides extracted from wild chrysanthemum in preparation of medicine for enhancing body immunity - Google Patents
Application of polysaccharides extracted from wild chrysanthemum in preparation of medicine for enhancing body immunity Download PDFInfo
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- CN102688255A CN102688255A CN2012102149647A CN201210214964A CN102688255A CN 102688255 A CN102688255 A CN 102688255A CN 2012102149647 A CN2012102149647 A CN 2012102149647A CN 201210214964 A CN201210214964 A CN 201210214964A CN 102688255 A CN102688255 A CN 102688255A
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Abstract
The invention belongs to the field of extraction of active medicinal ingredients for effectively enhancing body immunity from traditional Chinese medicinal materials. The technical scheme comprises the following steps of: preparing 500g of dried wild chrysanthemum medicinal materials, extracting in a water bath at 90 DEG C for 1 hour by using water as an extractant, continuously extracting for 3 times, filtering, combining the water extracts, concentrating, and then adding 95% ethanol which is 5 times of the concentrated extract, and standing for 24 hours; performing suction-filtration on precipitate obtained after ethanol precipitation, and then sequentially washing the precipitate with absolute ethanol, acetone and ethyl ether in sequence, and drying to obtain wild chrysanthemum crude polysaccharide powder; purifying the wild chrysanthemum crude polysaccharide powder by using LSA-21 macroporous adsorption resin, performing the same ethanol precipitation on the received solution to obtain relatively purified wild chrysanthemum polysaccharide powder, wherein the detection based on the anthrone-sulphuric acid method proves that the content of polysaccharides is 81.29%; preparing the wild chrysanthemum polysaccharide powder into wild chrysanthemum polysaccharide solution with concentration of 40-20 g.L<-1> by normal saline respectively during intragastric administration; and finally, combining the extracted wild chrysanthemum polysaccharides with common auxiliary materials to obtain various dosage forms of medicines for enhancing immunity.
Description
Technical field
The invention belongs to the active constituents of medicine that from the Chinese medicine material, extracts effective human body immunity improving power, especially will be used to prepare the medical usage of enhancing human body immunity power medicine from the polysaccharide component the Chinese crude drug Flos Chrysanthemi Indici.
Background technology
Along with society and expanding economy, increasing working clan is because the influence of factor such as overworked, anxious, hypomotility and diet be unbalance, thereby causes immunity of organisms low.How to seek a kind of not only safety but also cheap enhancing immunity from natural medicine, be the target that this area scientific research personnel strives to find always diligently.
Flos Chrysanthemi Indici is the head inflorescence of feverfew Herba Dendranthematis indici Chrysanthemum indicum L. HERBA CHRYSANTHEMI LAVANDULAEFOLII C.boreale Mak. or lavandulaleaf dendranthema herb C.lavandulaefolium (Fisch.) Mak..Be born in patana, shrubbery, river bank meadow bog, beach salt lick and limit, field, roadside are on the rock.Resource distribution: be distributed in ground such as Jilin, Liaoning, Hebei, Henan, Shanxi, Shaanxi, Gansu, Qinghai, Xinjiang, Shandong, Jiangsu, Zhejiang, Anhui, Fujian, Jiangxi, Hubei, Sichuan, Yunnan, Hunan.Flos Chrysanthemi Indici contains a large amount of polysaccharide components, has wide biological activity, like pharmacological actions such as antitumor, antioxidation, treatment diabetes, antiviral, radioprotective, adjusting sexual function, accent blood fat.The Flos Chrysanthemi Indici polysaccharide (Chrysanthemum indicum polysaccharide, CIP) have anti-aging with remove aspect effect such as reactive oxygen free radical, but the research of its immunoregulation effect is not appeared in the newspapers so far as yet.The present invention furthers investigate the effect of the enhancing immunity of herbal polysaccharide, for seeking novel immunomodulator and antitumor drug wide prospect is provided.
Summary of the invention
Technical problem to be solved by this invention is to utilize cheap conventional Chinese medicine material Flos Chrysanthemi Indici, therefrom extracts the Flos Chrysanthemi Indici polysaccharide, the natural drug that preparation has human body immunity improving power.
Technical scheme of the present invention: getting exsiccant Flos Chrysanthemi Indici medical material 500 g, is extractant with water, presses solid-liquid ratio 1:32, and lixiviate 1 h in 90 ℃ of water-baths extracts 3 times continuously, filters, and merges the water extract, and 95% ethanol that concentrates 5 times of amounts of back adding leaves standstill 24 h.Wash with dehydrated alcohol, acetone, ether successively behind the deposition sucking filtration behind the precipitate with ethanol, get Flos Chrysanthemi Indici crude polysaccharides powder after the drying.Then utilize the LSA-21 macroporous adsorbent resin, it is carried out purification, receive liquid by aforementioned alcohol precipitating method in 40 ℃ of temperature, pH 5, flow velocity 3 BV/h, Flos Chrysanthemi Indici polysaccharide powder that must be purer, it is 81.29% that anthrone-sulfuric acid process detects polyoses content.Using normal saline to be made into concentration respectively when irritating stomach (ig) is 40 ~ 20 gL
-1The Flos Chrysanthemi Indici polysaccharide solution.The Flos Chrysanthemi Indici polysaccharide component that at last extraction is obtained combines with adjuvant commonly used, prepares the medicine of the enhancing immunity of various dosage forms.
The specific embodiment
Embodiment 1
Instrument, reagent and material
1.1.1 animal
The ICR mice, male, body weight 18 ~ 22 g are provided by the Jiangsu Prov. Research Inst. Traditional Chinese Medical Experimental Animal Center, laboratory animal quality certification SCXK (Soviet Union) 2007-0001.
Instrument
Spectrumlab 752S ultraviolet-uisible spectrophotometer Prism Optical Technology Co
Prunus mume (sieb.) sieb.et zucc. Teller ten thousand/electronic balance Switzerland
Prunus mume (sieb.) sieb.et zucc. Teller 100,000/electronic balance Switzerland
HH-6 type digital display thermostat water bath Jintan City Ao Rui electrical apparatus factory
TDL-5-A type centrifuge Anting Scientific Instrument Factory, Shanghai
Microfuge 16 type centrifuges Germany Beckman Coulter
1.1.3 reagent
Cyclophosphamide for injection Hengrui Medicine Co., Ltd., Jiangsu Prov., lot number 11061421
Sodium chloride injection Nanjing Xiaoying Medicine Group Co.,Ltd, lot number 2011081805
Flos Chrysanthemi Indici: prepared slices of Chinese crude drugs factory of Nanjing Pharmaceutical limited company dinitrofluorobenzene (DNFB) solution: take by weighing the tool plug bottle that DNFB 50 mg place dried and clean; With the 5 mL acetone Oleum Sesami solution (1: 1) that prepare in advance; Pour the sealing of bottle blended rubber cloth into; Use fresh behind the mixing.
Complement (GPS): extract 4 guinea pig heart blood, male and female half and half are separated fresh serum, and are subsequent use after mixing, and face time spent normal saline dilution 1: 10.
Chicken red blood cell (5%CRBC): get fresh Sanguis Gallus domesticus, remove and defibrinate, the centrifugal packed red cells that gets faces the time spent with normal saline washing three times, is made into 5% suspension and gets final product.
Receive the reagent thing
The preparation of Flos Chrysanthemi Indici polysaccharide: getting exsiccant Flos Chrysanthemi Indici medical material 500 g, is extractant with water, presses solid-liquid ratio 1:32, and lixiviate 1 h in 90 ℃ of water-baths extracts 3 times continuously, filters, and merges the water extract, and 95% ethanol that adds 5 times of amounts after concentrating leaves standstill 24 h.Wash with dehydrated alcohol, acetone, ether successively behind the deposition sucking filtration behind the precipitate with ethanol, get Flos Chrysanthemi Indici crude polysaccharides powder after the drying.Then utilize the LSA-21 macroporous adsorbent resin, it is carried out purification, receive liquid by aforementioned alcohol precipitating method in 40 ℃ of temperature, pH 5, flow velocity 3 BV/h, Flos Chrysanthemi Indici polysaccharide powder that must be purer, it is 81.29% that anthrone-sulfuric acid process detects polyoses content.Using normal saline to be made into concentration during ig is 40 gL
-1, i.e. Flos Chrysanthemi Indici polysaccharide highly concentrated solution.
Experimental technique
1.2.1 animal divides into groups and administration
The ICR male mice is divided into 3 groups at random: normal control group, model group and Flos Chrysanthemi Indici polysaccharide high dose group, 10 every group.Each organizes mice ig every day 1 time, continuous ig 14 d, and normal control group and model group be ig normal saline 10 mLkg respectively
-1D
-1, isopyknic Flos Chrysanthemi Indici polysaccharide solution 400 mgkg of Flos Chrysanthemi Indici polysaccharide high dose group ig
-1D
-1In the beginning in the 10th day of ig, the normal control group is through intraperitoneal injection of saline 10 mLkg
-1D
-1, continuous 4 d; Model group and Flos Chrysanthemi Indici polysaccharide high dose group are respectively through isopyknic cyclophosphamide 100 mgkg of lumbar injection
-1D
-1, continuous 4 d.
Immunologic hypofunction mice carbon is cleaned up the detection of ability influence
Administration the 14th day, tail vein injection india ink 0.1 ml/10g behind ig 2 h is in 1 (t
1) and 5 (t
2) behind the min respectively through eye socket venous blood collection 20 μ L, add to 2 mL, 0.1% Na
2CO
3In, the mensuration absorbance in ultraviolet-uisible spectrophotometer 680 nm places (
A), after the blood sampling mice is taken off neck and put to death, win liver, spleen and thymus and weigh, be calculated as follows and clean up index (K) and phagocytic index (α), and calculate organ index.
K?=(logA
1-?logA
5)/(t
2-?t
1)=log(A
1/A
5)/4
α=body weight/(liver weight+spleen is heavy) * K
1/3
Organ index=organ weights (mg)/mice body weight (g)
1.2.3 detection to immunologic hypofunction mice delayed allergy influence
Administration was given every mouse web portion depilation, about 3 * 3 cm of area with 5% sodium sulfide solution in the time of the 9th day
2The 10th day, smear DNFB solution sensitization for every mouse web portion, carry out initial immunity (sensitization).4 d after the sensitization evenly smear the inside and outside two sides of mouse right ear exterior feature with DNFB solution and attack.Attack behind 24 h cervical vertebra dislocation and put to death mice, cut left and right sides auricular concha, take off the auricle of diameter 8 mm, weigh with card punch.The degree of the ear swelling degree of mice (the auris dextra sheet heavily deducts the heavy difference of left auricle) expression delayed allergy.
Detection to the influence of immunologic hypofunction mice serum hemolysin
Administration is in the time of the 7th day, and chicken red blood cell normal saline suspension 0.2 mL of every Mus lumbar injection 5% carries out immunity.After the immunity 7 days, pluck eyeball and get blood, centrifugal; Get serum with 100 times of normal saline dilutions; Get dilute serum 1 mL, mix with 5% chicken erythrocyte suspension, 0.5 mL, 10% complement, 0.5 mL, other establishes not that the blank pipe of increase serum compares; After 37 ℃ of thermostat water baths are incubated 30 min, the ice-water bath cessation reaction.Centrifugal, get supernatant and measure absorbance in ultraviolet-uisible spectrophotometer 540 nm places.
Statistical analysis
Data are represented with
; Carry out variance analysis with 19.0 pairs of data of SPSS, employing t check is compared between organizing.
P<0.05 statistical significance is arranged.
Experimental result
1.3.1 the influence that immunologic hypofunction mice carbon is cleaned up ability
Compare with the normal control group, all significantly decline of each index of model group (
P<0.01); Compare with model group, the carbon of Flos Chrysanthemi Indici polysaccharide high dose group mice cleans up index, phagocytic index and spleen index and thymus index all raises, difference appear significance (
P<0.01 or
P<0.05).The result sees table 1.
Table 1 Flos Chrysanthemi Indici polysaccharide high dose group to immunologic hypofunction mice carbon clean up ability influence (
, n=10)
| Group | Dosage/mgkg -1 | K | α | Spleen index | Thymus index |
| The normal control group | ? | 0.0335±0.0065 | 5.7432±0.8706 | 4.95±0.91 | 2.15±0.62 |
| Model group | ? | 0.0134±0.0038 1 | 4.2316±0.3672 1 | 3.22±0.73 1 | 0.87±0.32 1 |
| Flos Chrysanthemi Indici polysaccharide high dose group | 400 | 0.0278±0.0059 3 | 5.3970±0.7356 3 | 4.55±0.82 3 | 1.67±0.72 3 |
Annotate: compare with the normal control group,
1 P<0.01; Compare with model group,
3 P<0.01.
Influence to the delayed allergy of immunologic hypofunction mice
Compare with the normal control group, model group ear swelling degree significantly reduce (
P<0.01); Compare with model group, all significantly increases of the ear swelling degree of Flos Chrysanthemi Indici polysaccharide high dose group mice (
P<0.01).The result sees table 2.
Table 2 Flos Chrysanthemi Indici polysaccharide high dose group to the influence of immunologic hypofunction mice delayed allergy (
, n=10)
| Group | Dosage/mgkg -1 | Ear swelling degree/mg |
| The normal control group | ? | 5.67±1.23 |
| Model group | ? | 3.21±0.91 1 |
| Flos Chrysanthemi Indici polysaccharide high dose group | 400 | 5.23±0.98 3 |
Annotate: compare with the normal control group,
1 P<0.01; Compare with model group,
3 P<0.01.
Influence to immunologic hypofunction mice serum hemolysin
Compare with the normal control group, the model group serum hemolysin significantly descend (
P<0.01); Compare with model group, the serum hemolysin content of Flos Chrysanthemi Indici polysaccharide high dose group mice all increases, difference have significance (
P<0.01 or
P<0.05).The result sees table 3.
Table 3 Flos Chrysanthemi Indici polysaccharide high dose group to the influence of immunologic hypofunction mice serum hemolysin (
, n=10)
| Group | Dosage/mgkg -1 | Serum hemolysin |
| The normal control group | ? | 0.452±0.059 |
| Model group | ? | 0.299±0.032 1 |
| Flos Chrysanthemi Indici polysaccharide high dose group | 400 | 0.410±0.063 3 |
Annotate: compare with the normal control group,
1 P<0.01; Compare with model group,
3 P<0.01.
Discuss
This experiment utilizes the cyclophosphamide lumbar injection to prepare the mouse model of immunologic hypofunction, to estimate the Flos Chrysanthemi Indici immuno-modulating effect of polysaccharides.
This experimental result shows; The Flos Chrysanthemi Indici polysaccharide is in the carbon clearance test; Can improve and clean up index; Improve the cytophagous phagocytic index of mouse monokaryon, explain that the Flos Chrysanthemi Indici polysaccharide has tangible activation, potentiation to the reticuloendothelial system phagocytic function, prompting Flos Chrysanthemi Indici polysaccharide has potentiation to the mice non-specific immunity.The Flos Chrysanthemi Indici polysaccharide also can obviously improve spleen index and the thymus index of immunocompromised mice, shows that the Flos Chrysanthemi Indici polysaccharide can stimulate the rising of the immunity of organism organ index of correlation, increases the immunocompetence of body.In addition; Flos Chrysanthemi Indici polysaccharide high dose group is the delayed allergy intensity of the remarkable low mice of enhance immunity of ability all; And the mice serum hemolysin content that can obviously improve caused by cyclophosphamide reduces phenomenon; Promote the mice hemolytic antibody to generate, immunosuppressant has antagonism to caused by cyclophosphamide, explains that the Flos Chrysanthemi Indici polysaccharide can improve mice specific humoral immunity and cellular immune function.
High concentrationThe Flos Chrysanthemi Indici polysaccharide has immunological enhancement to caused by cyclophosphamide immunologic hypofunction mice.
Embodiment 2
Experimental apparatus, reagent and material except following different, all the other are all with embodiment 1, the difference of embodiment 2 and embodiment 1 is: receiving the reagent thing is that concentration is 20 gL
-1, isopyknic Flos Chrysanthemi Indici polysaccharide solution is 200 mgkg during ig
-1D
-1, promptly be divided into normal control group, model group and Flos Chrysanthemi Indici polysaccharide low dose group totally 3 groups.
The experimental result that embodiment 2 obtains is following:
2.3.1 the influence that immunologic hypofunction mice carbon is cleaned up ability
Compare with the normal control group, all significantly decline of each index of model group (
P<0.01); Compare with model group, the carbon of Flos Chrysanthemi Indici polysaccharide low dose group mice cleans up index, phagocytic index and spleen index and thymus index all raises, difference appear significance (
P<0.01 or
P<0.05).The result sees table 4.
Table 4 Flos Chrysanthemi Indici polysaccharide low dose group to immunologic hypofunction mice carbon clean up ability influence (
, n=10)
| Group | Dosage/mgkg -1 | K | α | Spleen index | Thymus index |
| The normal control group | ? | 0.0335±0.0065 | 5.7432±0.8706 | 4.95±0.91 | 2.15±0.62 |
| Model group | ? | 0.0134±0.0038 1 | 4.2316±0.3672 1 | 3.22±0.73 1 | 0.87±0.32 1 |
| Flos Chrysanthemi Indici polysaccharide low dose group | 200 | 0.0199±0.0047 2 | 4.8278±0.4801 2 | 3.78±0.77 2 | 1.02±0.59 2 |
Annotate: compare with the normal control group,
1 P<0.01; Compare with model group,
2 P<0.05.
Influence to the delayed allergy of immunologic hypofunction mice
Compare with the normal control group, model group ear swelling degree significantly reduce (
P<0.01); Compare with model group, all significantly increases of the ear swelling degree of Flos Chrysanthemi Indici polysaccharide low dose group mice (
P<0.01).The result sees table 5.
Table 5 Flos Chrysanthemi Indici polysaccharide low dose group to the influence of immunologic hypofunction mice delayed allergy (
, n=10)
| Group | Dosage/mgkg -1 | Ear swelling degree/mg |
| The normal control group | ? | 5.67±1.23 |
| Model group | ? | 3.21±0.91 1 |
| Flos Chrysanthemi Indici polysaccharide low dose group | 200 | 4.89±1.10 3 |
Annotate: compare with the normal control group,
1 P<0.01; Compare with model group,
3 P<0.01.
Influence to immunologic hypofunction mice serum hemolysin
Compare with the normal control group, the model group serum hemolysin significantly descend (
P<0.01); Compare with model group, the serum hemolysin content of Flos Chrysanthemi Indici polysaccharide low dose group mice all increases, difference have significance (
P<0.01 or
P<0.05).The result sees table 6.
Table 6 Flos Chrysanthemi Indici polysaccharide low dose group to the influence of immunologic hypofunction mice serum hemolysin (
, n=10)
| Group | Dosage/mgkg -1 | Serum hemolysin |
| The normal control group | ? | 0.452±0.059 |
| Model group | ? | 0.299±0.032 1 |
| Flos Chrysanthemi Indici polysaccharide low dose group | 200 | 0.357±0.058 2 |
Annotate: compare with the normal control group,
1 P<0.01; Compare with model group,
2 P<0.05.
Discuss
The data of embodiment 2 show that low concentration Flos Chrysanthemi Indici polysaccharide has immunological enhancement to caused by cyclophosphamide immunologic hypofunction mice.
Conclusion
The embodiment of the invention 1 utilizes cyclophosphamide to prepare the mouse model of immunologic hypofunction with embodiment 2; Observe of the influence of the Flos Chrysanthemi Indici polysaccharide of high and low concentration respectively to the clearance test of immunologic hypofunction mice carbon; Measure mice body weight and thymus, spleen weight and calculate thymus, spleen index; Adopt dinitrofluorobenzene inducing mouse delayed allergy method (ear swelling method) to measure cellular immune function, detect serum hemolysin content.In the experiment, all significantly reductions of each item index of model group mice (
P<0.01); The carbon of Flos Chrysanthemi Indici polysaccharide high and low dose group is cleaned up index and is respectively 0.0278 ± 0.0059,0.0199 ± 0.0047; Phagocytic index is respectively 5.3970 ± 0.7356,4.8278 ± 0.4801, and the ear swelling degree is respectively 5.23 ± 0.98 mg, 4.89 ± 1.10 mg, and serum hemolysin is respectively 0.410 ± 0.063,0.357 ± 0.058; And each index and model group are relatively, all have significant difference (
P<0.01 or
P<0.05).Experimental result shows that the Flos Chrysanthemi Indici polysaccharide of high and low concentration all can strengthen caused by cyclophosphamide immunologic hypofunction immune function of mice.
The Flos Chrysanthemi Indici polysaccharide that extraction is obtained combines with this area various dosage form adjuvants commonly used, prepares the medicine of the enhancing human body immunity power of various different dosage forms.
Claims (3)
1. the application of the polysaccharide that from Flos Chrysanthemi Indici, extracts in preparation human body immunity improving power medicine.
2. a kind of polysaccharide that from Flos Chrysanthemi Indici, extracts as claimed in claim 1 improves the application in the animal immunizing power medicine in preparation, and it is characterized by the extraction process below adopting: getting exsiccant Flos Chrysanthemi Indici medical material 500 g, is extractant with water; Press solid-liquid ratio 1:32; Lixiviate 1 h in 90 ℃ of water-baths extracts 3 times continuously, filters; Merge the water extract, 95% ethanol that concentrates 5 times of amounts of back adding leaves standstill 24 h; Wash with dehydrated alcohol, acetone, ether successively behind the deposition sucking filtration behind the precipitate with ethanol, get Flos Chrysanthemi Indici crude polysaccharides powder after the drying; Then utilize the LSA-21 macroporous adsorbent resin, it is carried out purification, receive liquid by aforementioned alcohol precipitating method in 40 ℃ of temperature, pH 5, flow velocity 3 BV/h, Flos Chrysanthemi Indici polysaccharide powder that must be purer, it is 81.29% that anthrone-sulfuric acid process detects polyoses content; Use normal saline to be made into concentration during ig respectively and be 40-20 gL
-1The Flos Chrysanthemi Indici polysaccharide solution.
3. a kind of polysaccharide that from Flos Chrysanthemi Indici, extracts as claimed in claim 2 improves the application in the animal immunizing power medicine in preparation; It is characterized by the Flos Chrysanthemi Indici polysaccharide component that extraction is obtained and combine, prepare the medicine of the enhancing immunity of various dosage forms with adjuvant commonly used.
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Cited By (5)
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|---|---|---|---|---|
| CN103329733A (en) * | 2013-06-21 | 2013-10-02 | 夏西超 | Methods for preparing entomogenous fungus conjugate and extracting polysaccharides of entomogenous fungus conjugate |
| CN103919830A (en) * | 2014-04-21 | 2014-07-16 | 中国药科大学 | Application of chrysanthemum aqueous extract to preparation of medicament for treating drunkenness |
| CN104140470A (en) * | 2013-05-08 | 2014-11-12 | 中国科学院上海药物研究所 | Chrysanthemum morifolium cv.Hangju polysaccharide and preparation method and use thereof |
| CN104292350A (en) * | 2014-09-17 | 2015-01-21 | 上海交通大学 | Extraction process and application of chrysanthemum polysaccharide |
| CN112279933A (en) * | 2020-11-30 | 2021-01-29 | 河南大学 | Chrysanthemum polysaccharide, extraction method and application thereof in preparing medicine for treating functional constipation |
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2012
- 2012-06-27 CN CN2012102149647A patent/CN102688255A/en active Pending
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| 李厚兵等: "大孔吸附树脂纯化野菊花多糖工艺", 《中国实验方剂学杂志》 * |
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| CN104140470A (en) * | 2013-05-08 | 2014-11-12 | 中国科学院上海药物研究所 | Chrysanthemum morifolium cv.Hangju polysaccharide and preparation method and use thereof |
| CN104140470B (en) * | 2013-05-08 | 2017-02-08 | 中国科学院上海药物研究所 | Chrysanthemum morifolium cv.Hangju polysaccharide and preparation method and use thereof |
| CN103329733A (en) * | 2013-06-21 | 2013-10-02 | 夏西超 | Methods for preparing entomogenous fungus conjugate and extracting polysaccharides of entomogenous fungus conjugate |
| CN103329733B (en) * | 2013-06-21 | 2015-03-18 | 南阳医学高等专科学校 | Methods for preparing entomogenous fungus conjugate and extracting polysaccharides of entomogenous fungus conjugate |
| CN103919830A (en) * | 2014-04-21 | 2014-07-16 | 中国药科大学 | Application of chrysanthemum aqueous extract to preparation of medicament for treating drunkenness |
| CN103919830B (en) * | 2014-04-21 | 2016-06-22 | 中国药科大学 | Chrysanthemum water extract treats the application in drunk medicine in preparation |
| CN104292350A (en) * | 2014-09-17 | 2015-01-21 | 上海交通大学 | Extraction process and application of chrysanthemum polysaccharide |
| CN104292350B (en) * | 2014-09-17 | 2017-05-24 | 上海交通大学 | Extraction process and application of chrysanthemum polysaccharide |
| CN112279933A (en) * | 2020-11-30 | 2021-01-29 | 河南大学 | Chrysanthemum polysaccharide, extraction method and application thereof in preparing medicine for treating functional constipation |
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Application publication date: 20120926 |