CN106749732A - Artemisia rupestris extraction method of polysaccharides - Google Patents
Artemisia rupestris extraction method of polysaccharides Download PDFInfo
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The present invention relates to a kind of Artemisia rupestris extraction method of polysaccharides, the method utilizes ultrasonic assistant extraction process, and combine the de- albumen of dynamic Flavonoids by Macroporous Adsorption Resin, recovery rate is high, process is simple, low cost, be adapted to industrialized production, the recovery rate of Artemisia rupestris polysaccharide crude is at least 3.11%, scavenging action when concentration is 2.5mg/mL to hydroxy radical and DPPH is above 60%, and the polysaccharide yield of purified polysaccharide crude product is at least 77.9%.
Description
Technical field
The present invention relates to a kind of extraction method of polysaccharides, and in particular to Artemisia rupestris extraction method of polysaccharides, belong to biological doctor
Medicine field.
Background technology
Polysaccharide (Polysaccharide, PS) is the macromolecular being polymerized by glycosidic bond by aldose or ketose, extensively
It is present in animal and plant and microorganism.Polysaccharide as a kind of important living matter, with anti-oxidant, anti-aging, it is antiviral,
The multiple biological activities such as antitumor, antifatigue, hypoglycemic.Nowadays oneself has many results of study to show, carbohydrate is used as information point
Son fertilization, occur, Development And Differentiation, the aspect such as the maintenance of nervous system plays an important role.In addition, inflammation and autoimmunity disease
Sick, aging, cancer cell abnormality proliferation and conversion, also have carbohydrate in the biological process such as cell recognition and pathogenic infection
Mediation.
Artemisia rupestris are a kind of herbaceos perennial of composite family artemisia, and bitter is cold in nature, is Uygur nationality's traditional medicine
Material, with expelling pathogenic wind and promoting blood circulation, clearing heat and detoxicating, eliminating stasis to subdue swelling, stomach strengthening and digestion promoting, antiallergy, antitumor, antiemetic, stop blooding, antidote against snake bite etc. it is many
Weight effect.Clinically it is used to treat cat fever, hepatitis, indigestion, nettle rash, anaphylactia etc..Uighur is referred to as
It is " a diligent Ai Manni ".
The main material such as including flavonoids, sesquiterpenoids, polysaccharide of Artemisia rupestris chemical composition.(one, the Xinjiang such as Xu Xin
The antioxidation in vitro Journal of Sex Research of wormwood artemisia polysaccharide, food science and technology, the 12nd phase of volume 36 in 2011) research show, Artemisia rupestris polysaccharide
Content is high, with anti-oxidant, activity of fighting against senium.Therefore the extraction process of Artemisia rupestris polysaccharide is optimized, for Artemisia rupestris
The comprehensive utilization of plant resources has great importance.
Existing Artemisia rupestris polysaccharide extracting process has water-boiling method, microwave assisting method, there is that recovery rate is low, technique is multiple
Miscellaneous, high cost, be not suitable for the defects such as industrialized production.
The content of the invention
The invention provides a kind of recovery rate is high, process is simple, low cost, the Artemisia rupestris that are adapted to industrialized production
Polysaccharide ultrasonic assistant extraction process.
According to an aspect of the present invention, there is provided a kind of Artemisia rupestris extraction method of polysaccharides, comprise the following steps：
(1) Artemisia rupestris are taken, be placed in concentration for 75-100% (preferably 85-98%, in ethanol most preferably 95%), heat
Flowed back under water-bath, dried.The step can remove lipid, oligosaccharide, monose and pigment.
(2) Artemisia rupestris dried in step (1) are taken to crush, is placed in water, Xinjiang one is extracted using ultrasonic assistant
Branch wormwood artemisia polysaccharide, concentrates after extract solution is filtered.
(3) take the extract solution after concentration in step (2), additions concentration for 75-100% (preferably 85-98%, most preferably
95%) ethanol, carries out alcohol precipitation, taking precipitate, dry Artemisia rupestris polysaccharide.The Artemisia rupestris polysaccharide for now obtaining is
Crude product.
In preferred technical scheme, extracting method of the invention further includes step (4) using dynamic macroporous absorption
Resin method is purified to take off albumen, must slough the Artemisia rupestris polysaccharide of floating preteins.
In preferred embodiments, in step (1), flow back 2-5 times, more preferably 2-3 times, most preferably 2 times；Each 0.5-
5h, more preferably each 1-3h, most preferably each 2h；Solid-liquid ratio is 1:2-1:7(g:ML), more preferably 1:3-1:5(g:ML), most
Preferably 1:4(g:mL).
In preferred embodiments, in step (2), the Artemisia rupestris of crushing cross 40-60 mesh sieves, more preferably cross 40-
50 mesh sieves, most preferably cross 40 mesh sieves；Solid-liquid ratio is 1:10-1:50(g:ML), more preferably 1:20-1:40(g:ML), most preferably
It is 1:30(g:mL)；Extracting temperature is 40-80 DEG C, most preferably more preferably 60-80 DEG C, 72 DEG C；Extraction time is 1-5h, more
Preferably 2-3h, most preferably 2.5h；Ultrasonic power is 200-500W, more preferably 300-400W, most preferably 350W；
Concentrated under water-bath, further preferred bath temperature is 70-90 DEG C, most preferably more preferably 75-85 DEG C, 80 DEG C.
In preferred embodiments, in step (3), the volume ratio of extract solution and ethanol after concentration is 1:3-1:5, more
Preferably 1:4-1:5, most preferably 1:4；The alcohol precipitation time is 4-24h, most preferably more preferably 8-16h, 12h；After alcohol precipitation from
The heart obtains sediment, more preferably 3000-4500r/min centrifugation 5-15min, most preferably 4000r/min centrifugations 10min.
In preferred embodiments, resin is HPD-300 resins, LS-46D resins or HP-600 resins in step (4)；
It is preferred that, by wet method dress post, addition concentration is that (preferably 2.0-3.0%, Artemisia rupestris most preferably 3.0%) are more for 1.5-4.0%
Sugar aqueous solution, with distillation water elution, must slough the Artemisia rupestris polysaccharide of floating preteins after collection.In further preferred implementation
In scheme, in step (4), the flow velocity of Artemisia rupestris polysaccharide solution is 0.5-2.5mL/min, more preferably 0.8-2.0mL/
Min, most preferably 1.0mL/min；The flow velocity of distilled water be 0.2-2.0mL/min, more preferably 0.3-1.5mL/min, it is optimal
Elect 0.5mL/min as.
According to another aspect of the present invention, the invention further relates to extract one, the Xinjiang for obtaining according to said extracted method
Wormwood artemisia polysaccharide.Artemisia rupestris polysaccharide of the invention can be used to prepare the health products and medicine of anti-aging.
Compared with prior art, it is an advantage of the invention that the recovery rate of Artemisia rupestris polysaccharide crude is at least 3.11%,
More traditional water-boiling method extraction process polysaccharide extract rate (1.67%) improves 1.64%.And low cost of the present invention, extraction conditions are accurate
Reliable, technological process is simple, is capable of achieving large-scale production.The Artemisia rupestris polysaccharide crude that the present invention is prepared is in concentration
Scavenging action during 2.5mg/mL to hydroxy radical and DPPH is above 60%.The method of purified polysaccharide crude product of the present invention, polysaccharide
Yield is at least 77.9%, and using the method for the de- protein method purified polysaccharide crude product of static Flavonoids by Macroporous Adsorption Resin, polysaccharide yield
Only 51.7%, using the method for enzyme process-de- protein method purified polysaccharide crude product of Sevage methods joint, polysaccharide yield is only
50.5%.
The present invention is described further below by specification drawings and specific embodiments.
Brief description of the drawings
The activity of Fig. 1 Artemisia rupestris polysaccharide crude scavenging hydroxyls of the invention and DPPH
Specific embodiment
With reference to specific embodiment, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate the present invention
Rather than limitation the scope of the present invention.Furthermore, it is to be understood that after described content of the invention has been read, this area skill
Art personnel can make various changes or modifications to the present invention, and these equivalent form of values equally fall within limited range of the present invention.
Embodiment 1
(1) Artemisia rupestris are taken, is placed in the ethanol that concentration is 95%, solid-liquid ratio is 1:4(g:ML), flowed back under hot bath
2 times, each 2h dries.
(2) Artemisia rupestris that will be dried in step (1) are crushed, and cross 40 mesh sieves, are placed in water, and are carried using ultrasonic assistant
Artemisia rupestris polysaccharide is taken, solid-liquid ratio is 1:30(g:ML), Extracting temperature is 72 DEG C, and extraction time is 2.5h, ultrasonic power
It is 350W, after extract solution is filtered, 80 DEG C of water-bath concentrations.
(3) extract solution after concentration in step (2) is taken, the concentration for adding 4 times of extracting liquid volumes is that 95% ethanol is carried out
Alcohol precipitation, the alcohol precipitation time is 12h, 4000r/min centrifugation 10min, obtains sediment, dry Artemisia rupestris polysaccharide.
(4) using the HPD-300 de- albumen of dynamic Flavonoids by Macroporous Adsorption Resin：Wet method dress post, adds the Xinjiang that concentration is 3.0%
Alpine yallow herb polysaccharide solution (flow velocity is 1.0mL/min), with distillation water elution (flow velocity is 0.5mL/min), must slough trip after collection
From the Artemisia rupestris polysaccharide of albumen.
In the present embodiment, polysaccharide crude recovery rate is 3.11%, and the method polysaccharide yield of purified polysaccharide crude product is 79.6%.
The computational methods of wherein polysaccharide crude recovery rate are：
Xinjiang Herba Achilleae polysaccharide crude recovery rate (%)=polysaccharide crude quality/material quality × 100%
The computational methods of the polysaccharide yield of purified polysaccharide method are：
Polysaccharide quality/polysaccharide crude the quality of Xinjiang Herba Achilleae purified polysaccharide yield (%)=slough floating preteins ×
100%
Embodiment 2
(1) Artemisia rupestris are taken, is placed in the ethanol that concentration is 95%, solid-liquid ratio is 1:4(g:ML), flowed back under hot bath
2 times, each 2h dries.
(2) Artemisia rupestris that will be dried in step (1) are crushed, and cross 40 mesh sieves, are placed in water, and are carried using ultrasonic assistant
Artemisia rupestris polysaccharide is taken, solid-liquid ratio is 1:20(g:ML), Extracting temperature is 70 DEG C, and extraction time is 2.5h, ultrasonic power
It is 350W, after extract solution is filtered, 80 DEG C of water-bath concentrations.
(3) extract solution after concentration in step (2) is taken, the concentration for adding 4 times of extracting liquid volumes is that 95% ethanol is carried out
Alcohol precipitation, the alcohol precipitation time is 12h, 4000r/min centrifugation 10min, obtains sediment, dry Artemisia rupestris polysaccharide.
(4) using the HPD-300 de- albumen of dynamic Flavonoids by Macroporous Adsorption Resin：Wet method dress post, adds the Xinjiang that concentration is 3.0%
Alpine yallow herb polysaccharide solution (flow velocity is 1.0mL/min), with distillation water elution (flow velocity is 0.5mL/min), must slough trip after collection
From the Artemisia rupestris polysaccharide of albumen.
In the present embodiment, polysaccharide crude recovery rate is 3.43%, and the method polysaccharide yield of purified polysaccharide crude product is 78.5%.
Embodiment 3
(1) Artemisia rupestris are taken, is placed in the ethanol that concentration is 95%, solid-liquid ratio is 1:5(g:ML), flowed back under hot bath
2 times, each 2h dries.
(2) Artemisia rupestris that will be dried in step (1) are crushed, and cross 40 mesh sieves, are placed in water, and are carried using ultrasonic assistant
Artemisia rupestris polysaccharide is taken, solid-liquid ratio is 1:10(g:ML), Extracting temperature is 80 DEG C, and extraction time is 2.5h, ultrasonic power
It is 350W, after extract solution is filtered, 80 DEG C of water-bath concentrations.
(3) extract solution after concentration in step (2) is taken, the concentration for adding 4 times of extracting liquid volumes is that 95% ethanol is carried out
Alcohol precipitation, the alcohol precipitation time is 12h, 4000r/min centrifugation 10min, obtains sediment, dry Artemisia rupestris polysaccharide.
(4) using the HPD-300 de- albumen of dynamic Flavonoids by Macroporous Adsorption Resin：Wet method dress post, adds the Xinjiang that concentration is 2.5%
Alpine yallow herb polysaccharide solution (flow velocity is 1.0mL/min), with distillation water elution (flow velocity is 0.5mL/min), must slough trip after collection
From the Artemisia rupestris polysaccharide of albumen.
In the present embodiment, polysaccharide crude recovery rate is 3.41%, and the method polysaccharide yield of purified polysaccharide crude product is 78.0%.
Embodiment 4
(1) Artemisia rupestris are taken, is placed in the ethanol that concentration is 95%, solid-liquid ratio is 1:3(g:ML), flowed back under hot bath
2 times, each 2.5h dries.
(2) Artemisia rupestris that will be dried in step (1) are crushed, and cross 40 mesh sieves, are placed in water, and are carried using ultrasonic assistant
Artemisia rupestris polysaccharide is taken, solid-liquid ratio is 1:30(g:ML), Extracting temperature is 60 DEG C, and extraction time is 3.0h, ultrasonic power
It is 350W, after extract solution is filtered, 80 DEG C of water-bath concentrations.
(3) extract solution after concentration in step (2) is taken, the concentration for adding 4 times of extracting liquid volumes is that 95% ethanol is carried out
Alcohol precipitation, the alcohol precipitation time is 12h, 4000r/min centrifugation 10min, obtains sediment, dry Artemisia rupestris polysaccharide.
(4) using the HPD-300 de- albumen of dynamic Flavonoids by Macroporous Adsorption Resin：Wet method dress post, adds the Xinjiang that concentration is 3.5%
Alpine yallow herb polysaccharide solution (flow velocity is 1.0mL/min), with distillation water elution (flow velocity is 0.5mL/min), must slough trip after collection
From the Artemisia rupestris polysaccharide of albumen.
In the present embodiment, polysaccharide crude recovery rate is 3.52%, and the method polysaccharide yield of purified polysaccharide crude product is 77.9%.
Embodiment 5
(1) Artemisia rupestris are taken, is placed in the ethanol that concentration is 95%, solid-liquid ratio is 1:4(g:ML), flowed back under hot bath
2 times, each 2h dries.
(2) Artemisia rupestris that will be dried in step (1) are crushed, and cross 40 mesh sieves, are placed in water, and are carried using ultrasonic assistant
Artemisia rupestris polysaccharide is taken, solid-liquid ratio is 1:30(g:ML), Extracting temperature is 80 DEG C, and extraction time is 2.0h, ultrasonic power
It is 300W, after extract solution is filtered, 80 DEG C of water-bath concentrations.
(3) extract solution after concentration in step (2) is taken, the concentration for adding 4 times of extracting liquid volumes is that 95% ethanol is carried out
Alcohol precipitation, the alcohol precipitation time is 12h, 4000r/min centrifugation 10min, obtains sediment, dry Artemisia rupestris polysaccharide.
(4) using the HPD-300 de- albumen of dynamic Flavonoids by Macroporous Adsorption Resin：Wet method dress post, adds the Xinjiang that concentration is 3.0%
Alpine yallow herb polysaccharide solution (flow velocity is 1.0mL/min), with distillation water elution (flow velocity is 0.5mL/min), must slough trip after collection
From the Artemisia rupestris polysaccharide of albumen.
In the present embodiment, polysaccharide crude recovery rate is 3.45%, and the method polysaccharide yield of purified polysaccharide crude product is 78.4%.
Embodiment 6
(1) Artemisia rupestris are taken, is placed in the ethanol that concentration is 95%, solid-liquid ratio is 1:4(g:ML), flowed back under hot bath
2 times, each 2h dries.
(2) Artemisia rupestris that will be dried in step (1) are crushed, and cross 40 mesh sieves, are placed in water, and are carried using ultrasonic assistant
Artemisia rupestris polysaccharide is taken, solid-liquid ratio is 1:30(g:ML), Extracting temperature is 70 DEG C, and extraction time is 2.5h, ultrasonic power
It is 400W, after extract solution is filtered, 80 DEG C of water-bath concentrations.
(3) extract solution after concentration in step (2) is taken, the concentration for adding 4 times of extracting liquid volumes is that 95% ethanol is carried out
Alcohol precipitation, the alcohol precipitation time is 12h, 4000r/min centrifugation 10min, obtains sediment, dry Artemisia rupestris polysaccharide.
(4) using the HPD-300 de- albumen of dynamic Flavonoids by Macroporous Adsorption Resin：Wet method dress post, adds the Xinjiang that concentration is 3.0%
Alpine yallow herb polysaccharide solution (flow velocity is 1.0mL/min), with distillation water elution (flow velocity is 0.5mL/min), must slough trip after collection
From the Artemisia rupestris polysaccharide of albumen.
In the present embodiment, polysaccharide crude recovery rate is 3.47%, and the method polysaccharide yield of purified polysaccharide crude product is 78.8%.
Embodiment 7
1. the measure of Artemisia rupestris polysaccharide crude free radical scavenging
Prepare mass concentration be respectively 0.1,0.3,0.5,1.0,1.5,2.0,2.5,3.0, one, the Xinjiang of 3.5mg/mL
Wormwood artemisia polysaccharide crude solution, 50 μ L various concentrations polysaccharide crude solution and the μ of 50 μ L 120 are added to each in 96 hole microtiter plates
The DPPH solution of mol/L, after lucifuge reaction half an hour, it is A that absorbance is surveyed at 517nms, each sample does 3 parallel examinations
Test.Calculate DPPH free radical scavenging activity formula as follows：
Clearance rate (%)=[1- (As-Ar)/Ao]×100
According to the method described above, it is measured as positive control using Vc (ascorbic acid).
In formula：AsIt is DPPH and the absorbance of polysaccharide crude solution reaction；ArFor DPPH blank (50 μ L polysaccharide crudes solution+
The ethanol of 50 μ L 95%) absorbance；AoNot add the absorbance of sugared blank solution (ethanol of 50 μ LDPPH+50 μ L 95%).
Scavenging action of the various concentrations Artemisia rupestris polysaccharide crude product solution to DPPH is determined, with VcFor the positive is right
According to ELIASA detection the results are shown in Table 1.
Clearance rate of the alpine yallow herb polysaccharide crude of table 1 to DPPH
2. Artemisia rupestris polysaccharide crude removes the measure of hydroxyl radical free radical activity
With ultra-pure water respectively compound concentration be 0.1,0.3,0.5,1.0,1.5,2.0, the Artemisia rupestris of 2.5mg/mL it is many
Sugared crude product solution, sequentially adds salicylic acid (9mmol/L), the H of 15 μ L of 15 μ L in 96 orifice plates2O2(8.8mmol/L)、15μL
FeSO4(9mmol/L), and various concentrations the μ L of polysaccharide crude solution 75, reaction 30min (37 DEG C), ultra-pure water is blank
Control group, 3 parallel repetitions.Under the conditions of 510nm, respective absorbance A is determined using ELIASA.According to the method described above,
With Vc(ascorbic acid) is measured as control.Calculate hydroxyl radical free radical clearance rate formula as follows：
Clearance rate (%)=(Ao-Ai)/Ao×100
In formula：AiIt is the absorbance of sample liquid reaction；AoIt is the absorbance of blank control group.
Various concentrations Artemisia rupestris polysaccharide crude solution is added in hydroxyl radical free radical system is removed, with VcFor the positive is right
According to ELIASA detection the results are shown in Table 2.
Clearance rate of the alpine yallow herb polysaccharide crude of table 2 to OH
More than, embodiments of the present invention are illustrated.But, the present invention is not limited to above-mentioned implementation method.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements done etc., should be included in guarantor of the invention
Within the scope of shield.
Claims (10)
1. a kind of Artemisia rupestris extraction method of polysaccharides, it is characterised in that comprise the following steps：
(1) Artemisia rupestris are taken, be placed in concentration for 75-100% (preferably 85-98%, in ethanol most preferably 95%), hot bath
Lower backflow, dries.
(2) Artemisia rupestris that will be dried in step (1) are crushed, and are placed in water, and Artemisia rupestris are extracted using ultrasonic assistant
Polysaccharide, concentrates after extract solution is filtered.
(3) take the extract solution after concentration in step (2), additions concentration for 75-100% (preferably 85-98%, most preferably 95%)
Ethanol, carries out alcohol precipitation, taking precipitate, dry Artemisia rupestris polysaccharide.
2. extracting method as claimed in claim 1, it is characterised in that further comprising the steps：
(4) purified to take off albumen using dynamic Flavonoids by Macroporous Adsorption Resin, the Artemisia rupestris polysaccharide of floating preteins must be sloughed.
3. extracting method as claimed in claim 1 or 2, it is characterised in that
It is preferred that, backflow 2-5 times, most preferably more preferably 2-3 times, 2 times in step (1)；Each 0.5-5h, more preferably each 1-3h,
Most preferably each 2h；
It is preferred that, solid-liquid ratio is 1 in step (1):2-1:7(g:ML), more preferably 1:3-1:5(g:ML), most preferably 1:4(g:
mL)。
4. the extracting method as any one of claim 1-3, it is characterised in that
It is preferred that, the Artemisia rupestris crushed in step (2) cross 40-60 mesh sieves, more preferably cross 40-50 mesh sieves, most preferably cross 40 mesh
Sieve；
It is preferred that, solid-liquid ratio is 1 in step (2):10-1:50(g:ML), more preferably 1:20-1:40(g:ML), most preferably 1:
30(g:mL)；
It is preferred that, Extracting temperature is 40-80 DEG C, most preferably more preferably 60-80 DEG C, 72 DEG C in step (2)；
It is preferred that, extraction time is 1-5h, most preferably more preferably 2-3h, 2.5h in step (2)；
It is preferred that, ultrasonic power is 200-500W, most preferably more preferably 300-400W, 350W in step (2)；
It is preferred that, in step (2) under water-bath concentrate, further preferred bath temperature be 70-90 DEG C, more preferably 75-85 DEG C, most
Preferably 80 DEG C.
5. the extracting method as any one of claim 1-4, it is characterised in that
It is preferred that, the volume ratio of extract solution and ethanol in step (3) after concentration is 1:3-1:5, more preferably 1:4-1:5, it is optimal
Elect 1 as:4；
It is preferred that, the alcohol precipitation time is 4-24h, most preferably more preferably 8-16h, 12h in step (3)；
It is preferred that, it is centrifuged after alcohol precipitation in step (3) and obtains sediment, more preferably 3000-4500r/min centrifugation 5-15min, most preferably
4000r/min is centrifuged 10min.
6. the extracting method as any one of claim 2-5, it is characterised in that resin is HPD-300 trees in step (4)
Fat, LS-46D resins or HP-600 resins.
7. extracting method as claimed in claim 6, it is characterised in that wet method dress post in step (4), addition concentration is 1.5-
4.0% (preferably 2.0-3.0%, Artemisia rupestris polysaccharide solution most preferably 3.0%), with water elution is distilled, must after collection
Slough the Artemisia rupestris polysaccharide of floating preteins.
8. extracting method as claimed in claim 7, it is characterised in that
It is preferred that, the flow velocity of Artemisia rupestris polysaccharide solution is 0.5-2.5mL/min, more preferably 0.8-2.0mL/ in step (4)
Min, most preferably 1.0mL/min；
It is preferred that, in step (4) flow velocity of distilled water be 0.2-2.0mL/min, more preferably 0.3-1.5mL/min, most preferably
0.5mL/min。
9. the extracting method as described in claim any one of 1-8 extracts the Artemisia rupestris polysaccharide for obtaining.
10. application of the Artemisia rupestris polysaccharide as claimed in claim 9 in the medicine or health products for preparing anti-aging.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107936130A (en) * | 2017-12-29 | 2018-04-20 | 云南中医学院 | A kind of short-pedicel aconite root polysaccharide and extracting method and application |
| CN107936130B (en) * | 2017-12-29 | 2020-05-12 | 云南中医药大学 | Achillea alpina polysaccharide, extraction method and application |
| CN113651899A (en) * | 2021-09-18 | 2021-11-16 | 广东粤微生物科技有限公司 | Ganoderma lucidum extract with low chroma and high polysaccharide content and preparation method thereof |
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