CN104127573A - Preparation method for pharmaceutical composition for treating breast cancer - Google Patents
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Abstract
The invention relates to a preparation method for a pharmaceutical composition for treating breast cancer. Scientific compatibility is performed, and hedyotis diffusa, raw atractylodes macrocephala, fructus hippophae fruit, commelina communis, radix glyccrhiza, herba siphonostegiae, herba swertiae mileensis, fructus lycii, jujube, radix codonopsis, radix paeoniae rubra and dried orange peel are performed. The pharmaceutical composition employs traditional has extremely strong efficacy of tonifying qi and securing the exterior, soothing the liver and regulating qi, activating blood and dissipating binds, and diminishing inflammation and relieving pain. The pharmaceutical composition is convenient to use, low in cost, and obvious in inhibiting effect on breast cancer.
Description
Technical field
The present invention relates to technical field of Chinese medicines, be specifically related to a kind of preparation method for the treatment of the Chinese medicine composition of breast carcinoma.
Background technology
Tumor has now become the healthy and disease more widely of serious threat human life, and the whole world has de novo malignancy 9,000,000 people every year.In state-owned 1,300,000,000 populations, annual nearly 1,600,000 de novo malignancy patients, annual nearly 1,300,000 people die from cancer, therefore the control of tumor has become the problem of extensively paying attention in world wide, countries in the world have been dropped into every year a large amount of human and material resources and have been studied, but up to now,, still not fully aware of to its cause of disease, pathology, Therapeutic Method there is no important breakthrough.Breast carcinoma is one of modal malignant tumor of women, and according to statistics, sickness rate accounts for the 7-10% of the various malignant tumor of whole body, is only second to uterus carcinoma in gynaecopathia.Its morbidity is normal relevant with heredity, and between 40-60 year, menopause, women's sickness rate of front and back was higher.Only the mammary gland patient of about 1-2% is male.Usually occur in the malignant tumor of breast glandular epithelium tissue.It is one of even life-threatening modal malignant tumor of a kind of women's of having a strong impact on physical and mental health.Breast carcinoma male is rare.Breast carcinoma be breast glandular epithelium under multiple cancerigenic factor effect, there is gene mutation, cause hyperplasia out of control.Because change has occurred for the biological behavior of cancerous cell, present unordered, unconfined neoplasm.The cancerous cell that its histology takes the form of a large amount of primitivizations is crowded into a group in unlimited proliferation and disorderly state, extruding erosion damage normal structure around, the normal structure structure of destruction breast.Because mammary gland is not human life activity's important organ, original position breast carcinoma is not fatal from theoretical speech, can reach healing by surgical resection.But because mammary glandular cell is just lost after undergoing mutation
Normocellular characteristic, organizational structure is disorderly, cell connects loose, cancerous cell is easy to come off free, with blood or lymph fluid etc., sends out whole body, forms early stage far-end and shifts, and has increased very large difficulty to the clinical cure of breast carcinoma.The transfer of whole body important organ all will directly threaten people's life as lung shifts, vertigo moves, bone transfer etc., so breast carcinoma is that seriously to jeopardize human body raw
The malignant disease of life.
Summary of the invention
The object of the invention is the deficiency for solving the problems of the technologies described above, a kind of preparation method for the treatment of the Chinese medicine composition of breast carcinoma is provided.
The traditional Chinese medical science thinks, breast belongs to stomach on meridians route distributes, and nipple belongs to liver.Lid liver is organ of general, and property happiness bar reaches, as is subject to direct stimulation, and the stagnation of QI is lost and dredged, and perverse and unreasonable manner is violated stomach, the resistance of stagnation of liver-QI stomach, and pathogens agglomerating meridians form " mastopathia ".Due to conception vessel and Liver Channel closely related, two through colleague and lower abdomen, and some acupuncture points on the human body of conception vessel is all met with Liver Channel as Qugu, the middle utmost point, Guan Yuan etc.When through water in the future in, abundance of blood in CHONG meridian, resembling of Liver Channel stasis is more outstanding, on see uncomfortable in chest, mammary swelling pain, nextly see lower abdominal distention pain.After water comes, the sea of blood is empty temporarily, and stagnation of liver-QI is alleviated, therefore mammary swelling abdominal distention is also flat.So periodically outbreak repeatedly, breast caking is not loose, and pain exacerbates, and the growth and decline with susceptible to anger.
The present invention is the deficiency solving the problems of the technologies described above, and the technical scheme adopting is: a kind of preparation method for the treatment of the Chinese medicine composition of breast carcinoma, comprises the following steps:
(1) according to parts by weight, get Herba Hedyotidis Diffusae 20-30 part, Rhizoma Atractylodis Macrocephalae 15-20 part, sea buckthorn fruit 15-20 part, Commeline communis 15-20 part, Radix Glycyrrhizae 10-15 part, siphonostegia chinensis 10-15 part, Herba Swertiae Mileensis 10-15 part, Fructus Lycii 10-15 part, Fructus Jujubae 5-10 part, Radix Codonopsis 5-10 part, Radix Paeoniae Rubra 5-10 part and Pericarpium Citri Reticulatae 5-10 part, standby;
(2) get Herba Hedyotidis Diffusae, add 80% ethanol of 8 times of weight, extract 3 times, each 1h, merge extractive liquid,, concentrated; Aqueous solution with petroleum ether extraction concentrate, remove petroleum ether layer, upper D-101 type macroporous resin column, loading concentration is 2.6-5.2mg/mL, absorption flow velocity according to 6mL/min carries out dynamic adsorption, wherein blade diameter length ratio is 1: 4, and water, 30% alcoholic solution, 45% alcoholic solution and 60% alcoholic solution carry out eluting with the flow velocity of 5mL/min respectively, collect 60% alcoholic solution eluting part evaporate to dryness, temperature is not higher than 60 ℃, after vacuum drying, obtain powder I, standby;
(3) get Rhizoma Atractylodis Macrocephalae clean dry, pulverize, use deionized water soaking at room temperature 2 hours, normal pressure decocts and within 1 hour, does not stop to stir, and filters 60 ℃ of concentrating under reduced pressure of filtrate, and then with 95% ethanol precipitate with ethanol 72 hours, centrifugal, precipitation water dissolution, centrifugal, deionized water dialysis 72 hours, 55 ℃ of concentrating under reduced pressure postlyophilizations, then be dissolved in water, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours again, centrifugal, precipitation water dissolution, then concentrating under reduced pressure, lyophilization, obtains powder II, standby;
(4) get sea buckthorn fruit, adding mass fraction is 95% ethanol, heating and refluxing extraction 2h at 70 ℃, filters alcohol extract, and slag discards, filtrate vacuum concentration is to extractum, by extractum chloroform extraction, insoluble matter adopts the ethanol heating for dissolving that mass fraction is 95%, the standing crystallization of room temperature, obtain powder III, standby;
(5) get Commeline communis, Radix Glycyrrhizae, siphonostegia chinensis, Herba Swertiae Mileensis, Fructus Lycii, Fructus Jujubae, Radix Codonopsis, Radix Paeoniae Rubra and Pericarpium Citri Reticulatae, the water that adds 8 times of above-mentioned raw materials medicine weight, soak after 30min, decoct 45min, add again 8 times of amounts of water, decoct 30min, filter, merge decoction liquor, concentrating under reduced pressure, drying under reduced pressure obtains dry extract, be ground into fine powder and cross 80 orders, then add powder I, powder II and powder III, after mix homogeneously, add again mannitol, lactose, aspartame and Mentholum, after mix homogeneously with 60% ethanol as binding agent granulation.
The bag filter that the bag filter using while dialysing in described step (3) is molecular cut off 1000DALTON.
When concentrated in described step (4), vacuum is-0.06MPa～-0.08MPa that temperature is 45 ℃～60 ℃.
beneficial effect
This Chinese medicine preparation kidney tonifying tonify deficiency, nourishing blood and promoting blood circulation, treating both the principal and secondary aspects of a disease, improves immunity of human body itself, nervous system regulation, activating blood circulation to dissipate blood stasis eliminating stagnation, can effectively suppress tumor growth, and with low cost, reparation technology is simple and easy to do.The present invention adopts the Chinese crude drug of different properties, has very strong invigorating QI to consolidate the body surface resistance, depressed liver-energy dispersing and QI regulating, blood circulation promoting and dispersing pathogen accumulation, anti-inflammatory analgetic effect, and Chinese medicine composition of the present invention is easy to use, and cost is low, and breast carcinoma is had to obvious inhibitory action.To improving pain that tumor body compressing causes and hemorrhagely thering is good clinical efficacy, and obviously improve patient's the general status such as spirit, appetite, sleep, and side effect is little, treatment safety, convenient, can palliate the agonizing sufferings, extending life, especially remarkable to improving the quality of life effect of patients with advanced cancer.Simultaneously this medicine can also with the radiotherapy of various cancers, chemotherapy in be used in conjunction with, there is heightening the effect of a treatment, alleviate the effect of side effect.To not accepting the patient with advanced cancer of radiotherapy, chemotherapy, operative treatment, this medicine is desirable selection.
The specific embodiment
embodiment 1
a preparation method for the treatment of the Chinese medicine composition of breast carcinoma, comprises the following steps:
(1) according to parts by weight, get 5 parts of 20 parts of Herba Hedyotidis Diffusaes, 15 parts of Rhizoma Atractylodis Macrocephalae, 15 parts of sea buckthorn fruits, 15 parts of Commeline communis, 10 parts, Radix Glycyrrhizae, 10 parts of siphonostegia chinensiss, 10 parts of Herba Swertiae Mileensises, 10 parts of Fructus Lycii, 5 parts, Fructus Jujubae, 5 parts of Radix Codonopsis, 5 parts of Radix Paeoniae Rubra and Pericarpium Citri Reticulataes, standby;
(2) get Herba Hedyotidis Diffusae, add 80% ethanol of 8 times of weight, extract 3 times, each 1h, merge extractive liquid,, concentrated; Aqueous solution with petroleum ether extraction concentrate, remove petroleum ether layer, upper D-101 type macroporous resin column, loading concentration is 2.6mg/mL, absorption flow velocity according to 6mL/min carries out dynamic adsorption, wherein blade diameter length ratio is 1: 4, and water, 30% alcoholic solution, 45% alcoholic solution and 60% alcoholic solution carry out eluting with the flow velocity of 5mL/min respectively, collect 60% alcoholic solution eluting part evaporate to dryness, temperature is not higher than 60 ℃, after vacuum drying, obtain powder I, standby;
(3) get Rhizoma Atractylodis Macrocephalae clean dry, pulverize, use deionized water soaking at room temperature 2 hours, normal pressure decocts and within 1 hour, does not stop to stir, and filters 60 ℃ of concentrating under reduced pressure of filtrate, and then with 95% ethanol precipitate with ethanol 72 hours, centrifugal, precipitation water dissolution, centrifugal, deionized water dialysis 72 hours, 55 ℃ of concentrating under reduced pressure postlyophilizations, then be dissolved in water, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours again, centrifugal, precipitation water dissolution, then concentrating under reduced pressure, lyophilization, obtains powder II, standby;
The bag filter using during dialysis is the bag filter of molecular cut off 1000DALTON.
(4) get sea buckthorn fruit, adding mass fraction is 95% ethanol, heating and refluxing extraction 2h at 70 ℃, filters alcohol extract, and slag discards, filtrate vacuum concentration is to extractum, by extractum chloroform extraction, insoluble matter adopts the ethanol heating for dissolving that mass fraction is 95%, the standing crystallization of room temperature, obtain powder III, standby;
When concentrated in described step (4), vacuum is-0.06MPa～-0.08MPa that temperature is 45 ℃～60 ℃.
(5) get Commeline communis, Radix Glycyrrhizae, siphonostegia chinensis, Herba Swertiae Mileensis, Fructus Lycii, Fructus Jujubae, Radix Codonopsis, Radix Paeoniae Rubra and Pericarpium Citri Reticulatae, the water that adds 8 times of above-mentioned raw materials medicine weight, soak after 30min, decoct 45min, add again 8 times of amounts of water, decoct 30min, filter, merge decoction liquor, concentrating under reduced pressure, drying under reduced pressure obtains dry extract, be ground into fine powder and cross 80 orders, then add powder I, powder II and powder III, after mix homogeneously, add again mannitol, lactose, aspartame and Mentholum (mannitol, lactose, the addition of aspartame and Mentholum is consistent with the addition in Chinese medicinal granular formulation preparation process in prior art, for auxiliary material, its addition is not protection main points of the present invention), after mix homogeneously with 60 ethanol as binding agent granulation.
embodiment 2
A preparation method for the treatment of the Chinese medicine composition of breast carcinoma, comprises the following steps:
(1) according to parts by weight, get 10 parts of 30 parts of Herba Hedyotidis Diffusaes, 20 parts of Rhizoma Atractylodis Macrocephalae, 20 parts of sea buckthorn fruits, 20 parts of Commeline communis, 15 parts, Radix Glycyrrhizae, 15 parts of siphonostegia chinensiss, 15 parts of Herba Swertiae Mileensises, 15 parts of Fructus Lycii, 10 parts, Fructus Jujubae, 10 parts of Radix Codonopsis, 10 parts of Radix Paeoniae Rubra and Pericarpium Citri Reticulataes, standby;
(2) get Herba Hedyotidis Diffusae, add 80% ethanol of 8 times of weight, extract 3 times, each 1h, merge extractive liquid,, concentrated; Aqueous solution with petroleum ether extraction concentrate, remove petroleum ether layer, upper D-101 type macroporous resin column, loading concentration is 2.6-5.2mg/mL, absorption flow velocity according to 6mL/min carries out dynamic adsorption, wherein blade diameter length ratio is 1: 4, and water, 30% alcoholic solution, 45% alcoholic solution and 60% alcoholic solution carry out eluting with the flow velocity of 5mL/min respectively, collect 60% alcoholic solution eluting part evaporate to dryness, temperature is not higher than 60 ℃, after vacuum drying, obtain powder I, standby;
(3) get Rhizoma Atractylodis Macrocephalae clean dry, pulverize, use deionized water soaking at room temperature 2 hours, normal pressure decocts and within 1 hour, does not stop to stir, and filters 60 ℃ of concentrating under reduced pressure of filtrate, and then with 95% ethanol precipitate with ethanol 72 hours, centrifugal, precipitation water dissolution, centrifugal, deionized water dialysis 72 hours, 55 ℃ of concentrating under reduced pressure postlyophilizations, then be dissolved in water, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours again, centrifugal, precipitation water dissolution, then concentrating under reduced pressure, lyophilization, obtains powder II, standby;
The bag filter using during dialysis is the bag filter of molecular cut off 1000DALTON.
(5) get sea buckthorn fruit, adding mass fraction is 95% ethanol, heating and refluxing extraction 2h at 70 ℃, filters alcohol extract, and slag discards, filtrate vacuum concentration is to extractum, by extractum chloroform extraction, insoluble matter adopts the ethanol heating for dissolving that mass fraction is 95%, the standing crystallization of room temperature, obtain powder III, standby;
When concentrated in described step (4), vacuum is-0.06MPa～-0.08MPa that temperature is 45 ℃～60 ℃.
(5) get Commeline communis, Radix Glycyrrhizae, siphonostegia chinensis, Herba Swertiae Mileensis, Fructus Lycii, Fructus Jujubae, Radix Codonopsis, Radix Paeoniae Rubra and Pericarpium Citri Reticulatae, the water that adds 8 times of above-mentioned raw materials medicine weight, soak after 30min, decoct 45min, add again 8 times of amounts of water, decoct 30min, filter, merge decoction liquor, concentrating under reduced pressure, drying under reduced pressure obtains dry extract, be ground into fine powder and cross 80 orders, then add powder I, powder II and powder III, after mix homogeneously, add again mannitol, lactose, aspartame and Mentholum, after mix homogeneously with 60 ethanol as binding agent granulation.
embodiment 3
A preparation method for the treatment of the Chinese medicine composition of breast carcinoma, comprises the following steps:
(1) according to parts by weight, get 9 parts of 25 parts of Herba Hedyotidis Diffusaes, 16 parts of Rhizoma Atractylodis Macrocephalae, 18 parts of sea buckthorn fruits, 16 parts of Commeline communis, 12 parts, Radix Glycyrrhizae, 13 parts of siphonostegia chinensiss, 14 parts of Herba Swertiae Mileensises, 12 parts of Fructus Lycii, 6 parts, Fructus Jujubae, 7 parts of Radix Codonopsis, 8 parts of Radix Paeoniae Rubra and Pericarpium Citri Reticulataes, standby;
(2) get Herba Hedyotidis Diffusae, add 80% ethanol of 8 times of weight, extract 3 times, each 1h, merge extractive liquid,, concentrated; Aqueous solution with petroleum ether extraction concentrate, remove petroleum ether layer, upper D-101 type macroporous resin column, loading concentration is 2.6-5.2mg/mL, absorption flow velocity according to 6mL/min carries out dynamic adsorption, wherein blade diameter length ratio is 1: 4, and water, 30% alcoholic solution, 45% alcoholic solution and 60% alcoholic solution carry out eluting with the flow velocity of 5mL/min respectively, collect 60% alcoholic solution eluting part evaporate to dryness, temperature is not higher than 60 ℃, after vacuum drying, obtain powder I, standby;
(3) get Rhizoma Atractylodis Macrocephalae clean dry, pulverize, use deionized water soaking at room temperature 2 hours, normal pressure decocts and within 1 hour, does not stop to stir, and filters 60 ℃ of concentrating under reduced pressure of filtrate, and then with 95% ethanol precipitate with ethanol 72 hours, centrifugal, precipitation water dissolution, centrifugal, deionized water dialysis 72 hours, 55 ℃ of concentrating under reduced pressure postlyophilizations, then be dissolved in water, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours again, centrifugal, precipitation water dissolution, then concentrating under reduced pressure, lyophilization, obtains powder II, standby;
The bag filter using during dialysis is the bag filter of molecular cut off 1000DALTON.
(6) get sea buckthorn fruit, adding mass fraction is 95% ethanol, heating and refluxing extraction 2h at 70 ℃, filters alcohol extract, and slag discards, filtrate vacuum concentration is to extractum, by extractum chloroform extraction, insoluble matter adopts the ethanol heating for dissolving that mass fraction is 95%, the standing crystallization of room temperature, obtain powder III, standby;
When concentrated in described step (4), vacuum is-0.06MPa～-0.08MPa that temperature is 45 ℃～60 ℃.
(5) get Commeline communis, Radix Glycyrrhizae, siphonostegia chinensis, Herba Swertiae Mileensis, Fructus Lycii, Fructus Jujubae, Radix Codonopsis, Radix Paeoniae Rubra and Pericarpium Citri Reticulatae, the water that adds 8 times of above-mentioned raw materials medicine weight, soak after 30min, decoct 45min, add again 8 times of amounts of water, decoct 30min, filter, merge decoction liquor, concentrating under reduced pressure, drying under reduced pressure obtains dry extract, be ground into fine powder and cross 80 orders, then add powder I, powder II and powder III, after mix homogeneously, add again mannitol, lactose, aspartame and Mentholum, after mix homogeneously with 60 ethanol as binding agent granulation.
clinical observation
Clinical data: be selected from the outpatient service in JIUYUE, 2010 to 2013 year May and inpatient totally 66 examples, wherein breast carcinoma of early stage 34 examples.Age is 32-56 year, average 42 years old.
Diagnostic criteria: the diagnostic criteria of the 8th fascicle < < breast carcinoma > > in the < < China common cancer diagnosis and treatment standard > > that China's Ministry of Public Health of take is compiled is foundation.
Therapeutic Method: take medicine of the present invention, the 20g that takes after mixing it with water every day, respectively once, the 10g that at every turn takes after mixing it with water, 15 days is 1 course for the treatment of sooner or later.
Efficacy assessment standard: according to breast carcinoma efficacy assessment standard in Ministry of Public Health < < China common cancer diagnosis and treatment standard > >, with before patient treatment with treatment after the super or CT of B compare, alleviating (CR), partial rcsponse (PR), stable (SD) completely, progress (PD) is carried out efficacy evaluation.
Therapeutic outcome: alleviate 55 examples completely, partial rcsponse 6 examples, stablize 4 examples, progress 1 example.Effective percentage (CR+PR) is 92.4%, and coefficient of stabilization (CR+PR+SD) is 98.48%.In early stage 34 routine breast carcinoma of early stage, there are 24 examples to cure (tumor all disappears, and follows up a case by regular visits to not recurrence in 1 year) simultaneously.
Toxic and side effects is analyzed
In the 66 routine patients that observe, only have 1 routine laxativeness after taking Chinese medicine of the present invention, the probability that occurs side reaction is only 1.5%, through disposing to the ill side reaction, disappears, and all the other are without any side effects.In sum, the present invention is used for the treatment of the medicine of breast carcinoma, through clinical trial, shows that it can effectively suppress breast cancer development, improves patient's symptom, sign, and without obvious toxic-side effects.
model case
Case 1: Song, 45 years old, lump in breast was found in patient's health check-up, and without obvious pain, also aperiodicity changes, and quality is hard as rock, and mobility is poor.Through hospital diagnosis, be right breast carcinoma, patient's emaciated physique, shortness of breath and palpitation, dim complexion, fatigue and weakness, soreness of the waist and knees.Because not wanting to be treated surgically, turn the traditional Chinese medical science and cure.Disease is seen through thing disorderly, premenstrual distending pain of the breast, and lump in breast is hard, pale tongue, white and thin fur, stringy and thready pulse.Take 2 courses for the treatment of of Chinese medicine of the present invention, the premenstrual distending pain of the breast of patient alleviates to some extent, and sleep improves.6 courses for the treatment of of following the service Chinese medicine composition of the present invention, the premenstrual distending pain of the breast of patient disappears, and menstrual cycle is normal, and lump in breast before dwindles.2 courses for the treatment of of following the service Chinese medicine composition of the present invention, lump in breast disappears, and all diseases are all removed, and examination in hospital is without difference.Follow up a case by regular visits to 1 year, health is good.
Case 2: Korea Spro, 56 years old, approximately 3 * 3.5cm of a lump was found in the outer top of left breast, matter firmly, not bitterly, rough, irregular around, hospital diagnosis is breast carcinoma.Disease is seen left arm distending pain, and slightly sense is numb sometimes, shallow complexion, tired mind, thin and greasy fur, stringy pulse.Take 2 courses for the treatment of of Chinese medicine composition of the present invention, patient's appetite increases, and sleep improves, and all diseases all have alleviation.Following the service Chinese medicine composition of the present invention 5 months, the left swelling of the breast piece of patient dwindles, and left arm distending pain, pain and numbness disappear.Following the service Chinese medicine composition of the present invention 4 months, after treatment, abnormal all diseases disappear substantially, and the index of tumor is all normal.Follow up a case by regular visits to 1 year, health is good.
Claims (3)
1. a preparation method for the treatment of the Chinese medicine composition of breast carcinoma, is characterized in that: comprise the following steps:
(1) according to parts by weight, get Herba Hedyotidis Diffusae 20-30 part, Rhizoma Atractylodis Macrocephalae 15-20 part, sea buckthorn fruit 15-20 part, Commeline communis 15-20 part, Radix Glycyrrhizae 10-15 part, siphonostegia chinensis 10-15 part, Herba Swertiae Mileensis 10-15 part, Fructus Lycii 10-15 part, Fructus Jujubae 5-10 part, Radix Codonopsis 5-10 part, Radix Paeoniae Rubra 5-10 part and Pericarpium Citri Reticulatae 5-10 part, standby;
(2) get Herba Hedyotidis Diffusae, add 80% ethanol of 8 times of weight, extract 3 times, each 1h, merge extractive liquid,, concentrated; Aqueous solution with petroleum ether extraction concentrate, remove petroleum ether layer, upper D-101 type macroporous resin column, loading concentration is 2.6-5.2mg/mL, absorption flow velocity according to 6mL/min carries out dynamic adsorption, wherein blade diameter length ratio is 1: 4, and water, 30% alcoholic solution, 45% alcoholic solution and 60% alcoholic solution carry out eluting with the flow velocity of 5mL/min respectively, collect 60% alcoholic solution eluting part evaporate to dryness, temperature is not higher than 60 ℃, after vacuum drying, obtain powder I, standby;
(3) get Rhizoma Atractylodis Macrocephalae clean dry, pulverize, use deionized water soaking at room temperature 2 hours, normal pressure decocts and within 1 hour, does not stop to stir, and filters 60 ℃ of concentrating under reduced pressure of filtrate, and then with 95% ethanol precipitate with ethanol 72 hours, centrifugal, precipitation water dissolution, centrifugal, deionized water dialysis 72 hours, 55 ℃ of concentrating under reduced pressure postlyophilizations, then be dissolved in water, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours, centrifugal, supernatant adds 95% ethanol precipitate with ethanol 24 hours again, centrifugal, precipitation water dissolution, then concentrating under reduced pressure, lyophilization, obtains powder II, standby;
(4) get sea buckthorn fruit, adding mass fraction is 95% ethanol, heating and refluxing extraction 2h at 70 ℃, filters alcohol extract, and slag discards, filtrate vacuum concentration is to extractum, by extractum chloroform extraction, insoluble matter adopts the ethanol heating for dissolving that mass fraction is 95%, the standing crystallization of room temperature, obtain powder III, standby;
(5) get Commeline communis, Radix Glycyrrhizae, siphonostegia chinensis, Herba Swertiae Mileensis, Fructus Lycii, Fructus Jujubae, Radix Codonopsis, Radix Paeoniae Rubra and Pericarpium Citri Reticulatae, the water that adds 8 times of above-mentioned raw materials medicine weight, soak after 30min, decoct 45min, add again 8 times of amounts of water, decoct 30min, filter, merge decoction liquor, concentrating under reduced pressure, drying under reduced pressure obtains dry extract, be ground into fine powder and cross 80 orders, then add powder I, powder II and powder III, after mix homogeneously, add again mannitol, lactose, aspartame and Mentholum, after mix homogeneously with 60% ethanol as binding agent granulation.
2. a kind of preparation method for the treatment of the Chinese medicine composition of breast carcinoma as claimed in claim 1, is characterized in that: the bag filter that the bag filter using while dialysing in described step (3) is molecular cut off 1000DALTON.
3. a kind of preparation method for the treatment of the Chinese medicine composition of breast carcinoma as claimed in claim 1, is characterized in that: when concentrated in described step (4), vacuum is-0.06MPa～-0.08MPa, and temperature is 45 ℃～60 ℃.
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Application publication date: 20141105 |